Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,482,222 A→T 15.9% intergenic (+21/+33) ynbD → / ← azoR putative phosphatase inner membrane protein/NADH‑azoreductase, FMN‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,482,2220AT15.9% 364.2 / 57.4 145intergenic (+21/+33)ynbD/azoRputative phosphatase inner membrane protein/NADH‑azoreductase, FMN‑dependent
Reads supporting (aligned to +/- strand):  ref base A (56/66);  new base T (8/15);  total (64/81)
Fisher's exact test for biased strand distribution p-value = 3.67e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.18e-01

Only 100 of 150 total aligned reads displayed.

CTATATTCGAGCCAGACGCCCGCAGATTGTGCTGACAGACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCTTTTGCGTCAGACTGTGCTTTCGCTGCCATTTCCGG  >  NC_000913/1482123‑1482320
                                                                                                   |                                                                                                  
ntataTTCGAGCCAGACGCCCGCAGATTGTGCTGACAGACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGa                                                                                                    >  1:1505655/2‑100 (MQ=255)
cTATATTCGAGCCAGACGCCCGCAGATTGTGCTGACAGACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGa                                                                                                    <  1:3725151/100‑1 (MQ=255)
  atatTCGAGCCAGACGCCCGCAGATTGTGCTGACAGACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAat                                                                                                  <  2:3189479/100‑1 (MQ=255)
   tatTCGAGCCAGACGCCCGCAGATTGTGCTGACAGACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAata                                                                                                 <  1:3765062/100‑1 (MQ=255)
    atTCGAGCCAGACGCCCGCAGATTGTGCTGACAGACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAatat                                                                                                >  2:3188002/1‑100 (MQ=255)
    atTCGAGCCAGACGCCCGCAGATTGTGCTGACAGACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAatat                                                                                                >  2:3524232/1‑100 (MQ=255)
    atTCGAGCCAGACGCCCGCAGATTGTGCTGACAGACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAatat                                                                                                >  1:1833450/1‑100 (MQ=255)
     ttCGAGCCAGACGCCCGCAGATTGTGCTGACAGACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATc                                                                                               <  1:1114535/100‑1 (MQ=255)
        gAGCCAGACGCCCGCAGATTGTGCTGACAGACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATc                                                                                               <  2:1548882/97‑1 (MQ=255)
        gAGCCAGACGCCCGCAGATTGTGCTGACAGACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTAc                                                                                            <  1:2310551/100‑1 (MQ=255)
        gAGCCAGACGCCCGCAGATTGTGCTGACAGACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTAc                                                                                            <  1:1274593/100‑1 (MQ=255)
        gAGCCAGACGCCCGCAGATTGTGCTGACAGAAGAGAACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTa                                                                                             <  1:619329/99‑1 (MQ=255)
          gCCAGACGCCCGCAGATTGTGCTGACAGACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAg                                                                                          <  2:2403450/100‑1 (MQ=255)
            cAGACGCCCGCAGATTGTGCTGACAGACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGt                                                                                         <  2:3348843/99‑1 (MQ=255)
             aGACGCCCGCAGATTGTGCTGACAGACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAGGTGGATTGAGATGTGGACTGAATATCTACAGTcc                                                                                       >  1:1471892/1‑100 (MQ=255)
             aGACGCCCGCAGATTGTGCTGACAGACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTcc                                                                                       <  1:3771947/100‑1 (MQ=255)
              gactccctgagagTGTGCTGACAGACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGt                                                                                         <  1:770661/88‑1 (MQ=255)
                cGCCCGCAGATTGTGCTGACAGACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGTAGATATTCAGTCcaca                                                                                    <  2:1090947/100‑1 (MQ=38)
                cGCCCGCAGATTGTGCTGACAGACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGTAGATATTCAGTCcaca                                                                                    <  1:2328120/100‑1 (MQ=38)
                cGCCCGCAGATTGTGCTGACAGACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGTAGATATTCAGTCcaca                                                                                    <  1:2542348/100‑1 (MQ=38)
                cGCCCGCAGATTGTGCTGACAGACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGTAGATATTCAGTCcaca                                                                                    <  1:1444818/100‑1 (MQ=38)
                cGCCCGCAGATTGTGCTGACAGACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCcaca                                                                                    >  1:1802222/1‑100 (MQ=255)
                cGCCCGCAGATTGTGCTGACAGACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCcaca                                                                                    <  1:814249/100‑1 (MQ=255)
                 gctcGCAGATTGTGCTGACAGACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGTAGATATTCAGTCCACAt                                                                                   <  2:1607258/97‑1 (MQ=38)
                 gCCCGCAGATTGTGCTGACAGACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGTAGATATTCAGTCCACAt                                                                                   <  2:2261328/100‑1 (MQ=38)
                 gCCCGCAGATTGTGCTGACAGACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGTAGATATTCAGTCCACAt                                                                                   <  1:3962403/100‑1 (MQ=38)
                 gCCCGCAGATTGTGCTGACAGACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGTAGATATTCAGTCCACAt                                                                                   <  2:3453180/100‑1 (MQ=38)
                  cccGCAGATTGTGCTGACAGACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGTAGATATTCAGTCCACATc                                                                                  <  1:2694434/100‑1 (MQ=38)
                  cccGCAGATTGTGCTGACAGACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGTAGATATTCAGTCCACATc                                                                                  <  1:3878235/100‑1 (MQ=38)
                  cccGCAGATTGTGCTGACAGACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGTAGATATTCAGTCCACATc                                                                                  <  2:4075455/100‑1 (MQ=38)
                  cccGCAGATTGTGCTGACAGACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATc                                                                                  >  2:8246/1‑100 (MQ=255)
                  cccGCAGATTGTGCTGACAGACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATc                                                                                  >  1:3309555/1‑100 (MQ=255)
                    cGCAGATTGTGCTGACAGACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGTAGATATTCAGTCCACATct                                                                                 <  2:2651062/99‑2 (MQ=37)
                      cAGATTGTGCTGACAGACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGTAGATATTCAGTCCACATctcaa                                                                              <  1:3081232/100‑5 (MQ=37)
                       aGATTGTGCTGACAGACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGAc                                                                             <  1:3860782/100‑1 (MQ=255)
                        gATTGTGCTGACAGACGAGCACAAAGCGATGCTGAGATTATGGGCAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACAACAAGAcc                                                                            >  2:4179850/1‑100 (MQ=255)
                        gATTGTGCTGACAGACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGAcn                                                                            <  2:1505652/100‑2 (MQ=255)
                        gATTGTGCTGACAGACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGAcc                                                                            >  1:2539523/1‑100 (MQ=255)
                         aTTGTGCTGACAGACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGAAAGTGGATTGAGATGAGGACTGAATATTTACAGTCCACATAAAGAc                                                                             >  2:1563277/1‑98 (MQ=255)
                          ttGTGCTGACAGACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCgt                                                                          >  1:3047386/1‑100 (MQ=255)
                               cTGACAGACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCg                                                                     >  1:1521451/1‑100 (MQ=255)
                                tGACAGACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCgg                                                                    >  1:3547706/1‑100 (MQ=255)
                                tGACAGACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCgg                                                                    <  1:2078224/100‑1 (MQ=255)
                                tGACAGACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCgg                                                                    >  2:1277215/1‑100 (MQ=255)
                                tGACAGACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAAGATCTACAGTCCACATCAAGACCGTGTCCgg                                                                    <  1:2653451/100‑1 (MQ=255)
                                 gACAGACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCggn                                                                   <  2:3164875/100‑2 (MQ=255)
                                 gACAGACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGt                                                                   <  1:1303175/100‑1 (MQ=255)
                                   cAGACGAGGACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTa                                                                 <  2:3891812/100‑1 (MQ=255)
                                   cAGACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTa                                                                 <  1:2593875/100‑1 (MQ=255)
                                   cAGACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTa                                                                 >  1:2652778/1‑100 (MQ=255)
                                        gAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAg                                                            <  1:1800323/100‑1 (MQ=255)
                                          gCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGaa                                                          >  1:672387/1‑100 (MQ=255)
                                            acaAAGCGATGCTGAGATTATGGGAAAACAGGTGAGTGGATTGAGATGTGGACTGAATATCTACAGTCCAGATCAAGACCGTGTCCGGTTATGCAGAAAc                                                        >  2:2524201/1‑100 (MQ=255)
                                               aaGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAAt                                                     <  2:2159338/100‑1 (MQ=255)
                                                 gCGATGCTGAGATTATGGGAAAACAGGTAGGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGc                                                   <  2:1471889/100‑1 (MQ=255)
                                                 gCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGGCTGAACATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGc                                                   <  1:4179879/100‑1 (MQ=255)
                                                 gCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGc                                                   <  2:1706093/100‑1 (MQ=255)
                                                  cGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCt                                                  <  1:3470463/100‑1 (MQ=255)
                                                      gCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCg                                              <  2:3792552/100‑1 (MQ=255)
                                                      gCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCg                                              <  2:3004309/100‑1 (MQ=255)
                                                       cTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGa                                             >  1:3745153/1‑100 (MQ=255)
                                                       cTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGa                                             >  2:1780581/1‑100 (MQ=255)
                                                          agaTTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATgg                                          <  2:648462/100‑1 (MQ=255)
                                                               aTGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGgctgct                                     >  2:1709431/1‑100 (MQ=255)
                                                                tGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCtt                                    >  2:1037654/1‑100 (MQ=255)
                                                                 gggAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCttt                                   <  2:3122021/100‑1 (MQ=255)
                                                                 gggAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCttt                                   >  2:982145/1‑100 (MQ=255)
                                                                 gggAAAACAGGTAAGTGGATTGAGATGTGGACTGAAGATCTACAGTCCACATCAAGACAGTGTCAGGTTATGCAGAAACAATGCTGTCGATGGCTGCttt                                   >  2:1967551/1‑100 (MQ=255)
                                                                  ggAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGCCCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCtttt                                  <  2:2382453/100‑1 (MQ=255)
                                                                     aaaCAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCTTTTGCg                               <  1:2234046/100‑1 (MQ=255)
                                                                     aaaCAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCTTTTGCg                               <  2:2531465/100‑1 (MQ=255)
                                                                       aCAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCTTTTGCGTc                             <  1:759592/100‑1 (MQ=255)
                                                                       aCAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCTTTTGCGTc                             <  1:2082087/100‑1 (MQ=255)
                                                                         aGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCTTTTGCGTCAg                           <  1:2171185/100‑1 (MQ=255)
                                                                          ggTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCTTTTGCGTCAGa                          >  1:2646838/1‑100 (MQ=255)
                                                                          ggTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCTTTTGCGTCAGa                          >  2:1981005/1‑100 (MQ=255)
                                                                           gTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCTTTTGCGTCAGAc                         >  2:3515823/1‑100 (MQ=255)
                                                                             aaGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCTTTTGCGTCAGACtg                       >  2:1840292/1‑100 (MQ=255)
                                                                              aGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCTTTTGCGTCAGACtgt                      >  1:818912/1‑100 (MQ=255)
                                                                              aGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCTTTTGCGTCAGACtgt                      >  1:2685857/1‑100 (MQ=255)
                                                                              aGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCTTTTGCGTCAGACtgt                      >  2:2142257/1‑100 (MQ=255)
                                                                               gTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCTTTTGCGTCAGACtgtg                     >  1:184764/1‑100 (MQ=255)
                                                                               gTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCTTTTGCGTCAGACtgtg                     >  2:1454216/1‑100 (MQ=255)
                                                                               gTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCTTTTGCGTCAGACtgtg                     <  2:228071/100‑1 (MQ=255)
                                                                               gTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCTTTTGCGTCAGACtgtg                     <  1:125709/100‑1 (MQ=255)
                                                                                   gtcttgaTGTGGACTGTAGATATTCAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCTTTTGCGTCAGACTGTGCttt                 >  1:3492772/6‑100 (MQ=25)
                                                                                    tcttgaTGTGGACTGTAGATATTCAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCTTTTGCGTCAGACTGTGCTTTc                >  2:1143560/5‑100 (MQ=37)
                                                                                      gagaTGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCTTTTGCGTCAGACTGTGCTTTCGc              <  1:3239213/100‑1 (MQ=255)
                                                                                       agaTGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCTTTTGCGTCAGACTGTGCTTTCGCt             <  2:205359/100‑1 (MQ=255)
                                                                                          tgtgGACTGTAGATATTCAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCTTTTGCGTCAGACTGTGCTTTCGCTGcc          >  2:1347204/1‑100 (MQ=38)
                                                                                          tgtgGACTGTAGATATTCAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCTTTTGCGTCAGACTGTGCTTTCGCTGcc          >  2:1208537/1‑100 (MQ=38)
                                                                                          tgtgGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCTTTTGCGTCAGACTTTGCTTTCGCTgtc          <  1:2845225/100‑3 (MQ=255)
                                                                                          tgtgGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCTTTTGCGTCAGACTGTGCTTTCGCTGcc          <  2:544434/100‑1 (MQ=255)
                                                                                            tgGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCTTTTGCGTCAGACTGTGCTTTCGCTGCCAt        <  1:4171812/100‑1 (MQ=255)
                                                                                              gACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCTTTTGCGTCAGACTGTGCTTTCGCTGCCAttt      >  1:3387456/1‑100 (MQ=255)
                                                                                                cTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCTTTTGCGTCAGACTGTGCTTTCGCTGCCATTTcc    <  1:319946/100‑1 (MQ=255)
                                                                                                cTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGACGGATGCTTTTGCGTCCGACTGTGCtttag               >  2:2750910/1‑87 (MQ=255)
                                                                                                 tGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCTTTTGCGTCAGACTGTGCTTTCGCTGCCATTTCCg   <  2:2646831/100‑1 (MQ=255)
                                                                                                 tGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCTTTTGCGTCAGACTGTGCTTTCGCTGCCATTTCCg   <  2:2187101/100‑1 (MQ=255)
                                                                                                  gAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCTTTTGCGTCAGACTGTGCTTTCGCTGCCATTTCCgg  >  1:2902933/1‑100 (MQ=255)
                                                                                                   |                                                                                                  
CTATATTCGAGCCAGACGCCCGCAGATTGTGCTGACAGACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCTTTTGCGTCAGACTGTGCTTTCGCTGCCATTTCCGG  >  NC_000913/1482123‑1482320

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: