Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,482,224 T→G 17.6% intergenic (+23/+31) ynbD → / ← azoR putative phosphatase inner membrane protein/NADH‑azoreductase, FMN‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,482,2240TG17.6% 323.7 / 65.0 140intergenic (+23/+31)ynbD/azoRputative phosphatase inner membrane protein/NADH‑azoreductase, FMN‑dependent
Reads supporting (aligned to +/- strand):  ref base T (52/62);  new base G (9/16);  total (61/79)
Fisher's exact test for biased strand distribution p-value = 5.05e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.37e-01

Only 100 of 152 total aligned reads displayed.

ATATTCGAGCCAGACGCCCGCAGATTGTGCTGACAGACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCTTTTGCGTCAGACTGTGCTTTCGCTGCCATTTCCGGAC  >  NC_000913/1482125‑1482322
                                                                                                   |                                                                                                  
atatTCGAGCCAGACGCCCGCAGATTGTGCTGACAGACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAat                                                                                                    <  2:3189479/100‑1 (MQ=255)
 tatTCGAGCCAGACGCCCGCAGATTGTGCTGACAGACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAata                                                                                                   <  1:3765062/100‑1 (MQ=255)
  atTCGAGCCAGACGCCCGCAGATTGTGCTGACAGACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAatat                                                                                                  >  2:3524232/1‑100 (MQ=255)
   ttCGAGCCAGACGCCCGCAGATTGTGCTGACAGACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATc                                                                                                 <  1:1114535/100‑1 (MQ=255)
   ttCGAGCCAGACGCCCGCAGATTGTGCTGACAGACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATc                                                                                                 <  2:2463713/100‑1 (MQ=255)
     cGAGCCAGACGCCCGCAGATTGTGCTGACAGACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTa                                                                                               <  2:3507345/100‑1 (MQ=255)
      gAGCCAGACGCCCGCAGATTGTGCTGACAGACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATc                                                                                                 <  2:1548882/97‑1 (MQ=255)
      gAGCCAGACGCCCGCAGATTGTGCTGACAGACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTAc                                                                                              <  1:1274593/100‑1 (MQ=255)
      gAGCCAGACGCCCGCAGATTGTGCTGACAGAAGAGAACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTa                                                                                               <  1:619329/99‑1 (MQ=255)
        gCCAGACGCCCGCAGATTGTGCTGACAGACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAg                                                                                            <  2:2403450/100‑1 (MQ=255)
           aGACGCCCGCAGATTGTGCTGACAGACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAGGTGGATTGAGATGTGGACTGAATATCTACAGTcc                                                                                         >  1:1471892/1‑100 (MQ=255)
           aGACGCCCGCAGATTGTGCTGACAGACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTcc                                                                                         <  1:3771947/100‑1 (MQ=255)
            gactccctgagagTGTGCTGACAGACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGt                                                                                           <  1:770661/88‑1 (MQ=255)
              cGCCCGCAGATTGTGCTGACAGACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGTAGATATTCAGTCcaca                                                                                      <  1:1444818/100‑1 (MQ=38)
              cGCCCGCAGATTGTGCTGACAGACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGTAGATATTCAGTCcaca                                                                                      <  1:2542348/100‑1 (MQ=38)
              cGCCCGCAGATTGTGCTGACAGACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGTAGATATTCAGTCcaca                                                                                      <  1:2328120/100‑1 (MQ=38)
              cGCCCGCAGATTGTGCTGACAGACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCcaca                                                                                      >  1:1802222/1‑100 (MQ=255)
              cGCCCGCAGATTGTGCTGACAGACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCcaca                                                                                      <  1:814249/100‑1 (MQ=255)
               gctcGCAGATTGTGCTGACAGACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGTAGATATTCAGTCCACAt                                                                                     <  2:1607258/97‑1 (MQ=38)
               gCCCGCAGATTGTGCTGACAGACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGTAGATATTCAGTCCACAt                                                                                     <  2:3453180/100‑1 (MQ=38)
               gCCCGCAGATTGTGCTGACAGACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGTAGATATTCAGTCCACAt                                                                                     <  1:3962403/100‑1 (MQ=38)
                cccGCAGATTGTGCTGACAGACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGTAGATATTCAGTCCACATc                                                                                    <  1:2694434/100‑1 (MQ=38)
                cccGCAGATTGTGCTGACAGACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGTAGATATTCAGTCCACATc                                                                                    <  1:3878235/100‑1 (MQ=38)
                cccGCAGATTGTGCTGACAGACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATc                                                                                    >  1:3309555/1‑100 (MQ=255)
                  cGCAGATTGTGCTGACAGACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGTAGATATTCAGTCCACATct                                                                                   <  2:2651062/99‑2 (MQ=37)
                     aGATTGTGCTGACAGACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGAc                                                                               <  1:3860782/100‑1 (MQ=255)
                      gATTGTGCTGACAGACGAGCACAAAGCGATGCTGAGATTATGGGCAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACAACAAGAcc                                                                              >  2:4179850/1‑100 (MQ=255)
                      gATTGTGCTGACAGACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGAcn                                                                              <  2:1505652/100‑2 (MQ=255)
                       aTTGTGCTGACAGACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGAAAGTGGATTGAGATGAGGACTGAATATTTACAGTCCACATAAAGAc                                                                               >  2:1563277/1‑98 (MQ=255)
                        ttGTGCTGACAGACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCgt                                                                            >  1:3047386/1‑100 (MQ=255)
                          gtgCTGACAGACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCgtgt                                                                          >  1:2566794/1‑100 (MQ=255)
                           tgCTGACAGACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTc                                                                         >  2:2028342/1‑100 (MQ=255)
                             cTGACAGACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCg                                                                       >  1:1521451/1‑100 (MQ=255)
                              tGACAGACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCgg                                                                      >  1:3547706/1‑100 (MQ=255)
                              tGACAGACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCgg                                                                      >  2:1277215/1‑100 (MQ=255)
                              tGACAGACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCgg                                                                      <  1:2651706/100‑1 (MQ=255)
                              tGACAGACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAAGATCTACAGTCCACATCAAGACCGTGTCCgg                                                                      <  1:2653451/100‑1 (MQ=255)
                               gACAGACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCggn                                                                     <  2:3164875/100‑2 (MQ=255)
                               gACAGACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGt                                                                     <  1:1303175/100‑1 (MQ=255)
                                 cAGACGAGGACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTa                                                                   <  2:3891812/100‑1 (MQ=255)
                                    aCGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGc                                                                <  2:747625/100‑1 (MQ=255)
                                        gCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGaa                                                            >  1:672387/1‑100 (MQ=255)
                                         cacaAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGaaa                                                           >  2:4069190/1‑100 (MQ=255)
                                           caAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACa                                                         >  2:1264946/1‑100 (MQ=255)
                                             aaGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAAt                                                       <  2:2159338/100‑1 (MQ=255)
                                               gCGATGCTGAGATTATGGGAAAACAGGTAGGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGc                                                     <  2:1471889/100‑1 (MQ=255)
                                               gCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGGCTGAACATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGc                                                     <  1:4179879/100‑1 (MQ=255)
                                               gCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGc                                                     >  2:3583996/1‑100 (MQ=255)
                                               gCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGc                                                     >  1:2288841/1‑100 (MQ=255)
                                               gCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGc                                                     <  2:1706093/100‑1 (MQ=255)
                                                cGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCt                                                    <  1:3470463/100‑1 (MQ=255)
                                                    gCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCg                                                <  2:3004309/100‑1 (MQ=255)
                                                     cTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGa                                               >  1:3745153/1‑100 (MQ=255)
                                                     cTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGa                                               >  2:1780581/1‑100 (MQ=255)
                                                     cTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGa                                               >  1:2028958/1‑100 (MQ=255)
                                                        agaTTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATgg                                            <  2:648462/100‑1 (MQ=255)
                                                             aTGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGgctgct                                       >  2:1709431/1‑100 (MQ=255)
                                                              tGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCtt                                      >  2:1037654/1‑100 (MQ=255)
                                                               tgggaaaCAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATAAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCttt                                     >  1:1820609/5‑100 (MQ=255)
                                                               gggAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCttt                                     <  1:3428586/100‑1 (MQ=255)
                                                               gggAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCttt                                     >  2:982145/1‑100 (MQ=255)
                                                               gggAAAACAGGTAAGTGGATTGAGATGTGGACTGAAGATCTACAGTCCACATCAAGACAGTGTCAGGTTATGCAGAAACAATGCTGTCGATGGCTGCttt                                     >  2:1967551/1‑100 (MQ=255)
                                                                ggAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGCCCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCtttt                                    <  2:2382453/100‑1 (MQ=255)
                                                                   aaaCAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCTTTTGCg                                 <  1:2234046/100‑1 (MQ=255)
                                                                   aaaCAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCTTTTGCg                                 <  2:2531465/100‑1 (MQ=255)
                                                                     aCAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCTTTTGCGTc                               <  1:759592/100‑1 (MQ=255)
                                                                     aCAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCTTTTGCGTc                               <  1:2082087/100‑1 (MQ=255)
                                                                     aCAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCTTTTGCGTc                               <  2:439993/100‑1 (MQ=255)
                                                                        ggTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCTTTTGCGTCAGa                            >  1:2646838/1‑100 (MQ=255)
                                                                         gTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCTTTTGCGTCAGAc                           <  1:3193080/100‑1 (MQ=255)
                                                                          tAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCTTTTGCGTCAGACt                          >  1:3019524/1‑100 (MQ=255)
                                                                           aaGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCTTTTGCGTCAGACtg                         >  2:1840292/1‑100 (MQ=255)
                                                                             gTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCTTTTGCGTCAGACtgtg                       <  1:125709/100‑1 (MQ=255)
                                                                             gTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCTTTTGCGTCAGACtgtg                       <  2:228071/100‑1 (MQ=255)
                                                                             gTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCTTTTGCGTCAGACtgtg                       >  1:184764/1‑100 (MQ=255)
                                                                             gTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCTTTTGCGTCAGACtgtg                       >  2:1454216/1‑100 (MQ=255)
                                                                               ggATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCTTTTGCGTCAGACTGTGCt                     >  1:849723/1‑100 (MQ=255)
                                                                               ggATTGAGATGTGGACTGAATATCTACAGACCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTCCTTTTGCGTCAGACTGTGCt                     <  2:2555110/100‑1 (MQ=255)
                                                                                  tcttgaTGTGGACTGTAGATATTCAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCTTTTGCGTCAGACTGTGCTTTc                  >  2:1143560/5‑100 (MQ=37)
                                                                                    gagaTGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCTTTTGCGTCAGACTGTGCTTTCGc                <  1:3239213/100‑1 (MQ=255)
                                                                                     agaTGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCTTTTGCGTCAGACTGTGCTTTCGCt               <  2:205359/100‑1 (MQ=255)
                                                                                      gaTGTGGACTGTAGATATTCAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCTTTTGCGTCAGACTGTGCTTTCGCTg              >  1:277857/1‑100 (MQ=38)
                                                                                       aTGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCTTTTGCGTCAGACTGTGCTTTCGCTGc             >  2:2152415/1‑100 (MQ=255)
                                                                                       aTGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCTTTTGCGTCAGACTGTGCTTTCGCTGc             <  1:3510813/100‑1 (MQ=255)
                                                                                        tgtgGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCTTTTGCGTCAGACTTTGCTTTCGCTgtc            <  1:2845225/100‑3 (MQ=255)
                                                                                        tgtgGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCTTTTGCGTCAGACTGTGCTTTCGCTGcc            <  1:1674961/100‑1 (MQ=255)
                                                                                         gtgGACTGTAGATATTCAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCTTTTGCGTCAGACTGTGCTTTCGCTGCCa           >  2:3180080/1‑100 (MQ=38)
                                                                                          tgGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCTTTTGCGTCAGACTGTGCTTTCGCTGCCAt          <  1:4171812/100‑1 (MQ=255)
                                                                                            gACTGAATATCTACAGGCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCTTTTGCGTCAGACTGTGCTTTCGCTGCCAttt        >  2:3018275/1‑100 (MQ=255)
                                                                                             aCTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCTTTTGCGTCAGACTGTGCTTTCGCTGCCATTTc       <  2:1133674/100‑1 (MQ=255)
                                                                                             aCTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCTTTTGCGTCAGACTGTGCTTTCGCTGCCATTTc       >  2:3273880/1‑100 (MQ=255)
                                                                                              cTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCTTTTGCGTCAGACTGTGCTTTCGCTGCCATTTcc      <  1:319946/100‑1 (MQ=255)
                                                                                              cTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGACGGATGCTTTTGCGTCCGACTGTGCtttag                 >  2:2750910/1‑87 (MQ=255)
                                                                                               tGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCTTTTGCGTCAGACTGTGCTTTCGCTGCCATTTCCg     <  1:2152418/100‑1 (MQ=255)
                                                                                               tGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCTTTTGCGTCAGACTGTGCTTTCGCTGCCATTTCCg     <  2:2187101/100‑1 (MQ=255)
                                                                                               tGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCTTTTGCGTCAGACTGTGCTTTCGCTGCCATTTCCg     <  2:2187200/100‑1 (MQ=255)
                                                                                                gAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCTTTTGCGTCAGACTGTGCTTTCGCTGCCATTTCCgg    >  1:2677060/1‑100 (MQ=255)
                                                                                                  atatCTACAGTCCGCATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCGTTTGCGTCAGACTGTGCTTTCGCTGCCATTTCCGGAc  >  2:188267/1‑100 (MQ=255)
                                                                                                  atatCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCTTTTGCGTCAGACTGTGCTTTCGCTGCCATTTCCGGAc  <  2:2485383/100‑1 (MQ=255)
                                                                                                  atatCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCTTTTGCGTCAGACTGTGCTTTCGCTGCCATTTCCGGAc  <  1:2323437/100‑1 (MQ=255)
                                                                                                   |                                                                                                  
ATATTCGAGCCAGACGCCCGCAGATTGTGCTGACAGACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCTTTTGCGTCAGACTGTGCTTTCGCTGCCATTTCCGGAC  >  NC_000913/1482125‑1482322

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: