Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,689,821 T→G 19.2% intergenic (+70/‑31) manA → / → ydgA mannose‑6‑phosphate isomerase/DUF945 family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,689,8210TG19.2% 298.4 / 63.4 129intergenic (+70/‑31)manA/ydgAmannose‑6‑phosphate isomerase/DUF945 family protein
Reads supporting (aligned to +/- strand):  ref base T (56/48);  new base G (15/10);  total (71/58)
Fisher's exact test for biased strand distribution p-value = 6.58e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 131 total aligned reads displayed.

GTTTAGCGCGTGTTTACAACAAGCTGTAAGAGCTTACTGAAAAAATTAACATCTCTTGCTAAGCTTATTAAAGGCTTATAACACCTTCAGGCGGCCAGTCCGCCTGATTTCATTTTATGGATAATCATTATGAATAAATCGCTGGTAGCGGTAGGCGTCATTGTTGCGCTAGGCGTAGTCTGGACAGGCGGCGCATGG  >  NC_000913/1689723‑1689920
                                                                                                  |                                                                                                   
gTTTAGCGCGTGTTTACAACAAGCTGTAAGAGCTTACTGAAAAAATTAACATCTCTTGCTAAGCTTATTAAAGGCTTATAACACCTTCAGGCGGCCAGTc                                                                                                    <  1:3597708/100‑1 (MQ=255)
 tttAGCGCGTGTTTACAACAAGCTGTAAGAGCTTACTGAAAAAATTAACATCTCTTGCTAAGCTTATTAAAGGCTTATAACACCTTCAGGCGGCCAGTcc                                                                                                   <  1:3784034/100‑1 (MQ=255)
   tAGCGCGTGTTTACAACAAGCTGTAAGAGCTTACTGAAAAAATTAACATCTCTTGCTAAGCTTATTAAAGGCTTATAACACCTTCAGGCGGCCAGTCCGc                                                                                                 <  2:3669835/100‑1 (MQ=255)
   tAGCGCGTGTTTACAACAAGCTGTAAGAGCTTACTGAAAAAATTAACATCTCTTGCTAAGCTTATTAAAGGCTTATAACACCTTCAGACGGCCAGTCCGc                                                                                                 >  1:1141321/1‑100 (MQ=255)
      cgcgTGTTTACAACAAGCTGTAAGAGCTTACTGAAAAAATTAACATCTCTTGCTAAGCTTATTAAAGGCTTATAACACCTTCAGGCGGCCAGTCCGCCTg                                                                                              >  1:801402/1‑100 (MQ=255)
      cgcgTGTTTACAACAAGCTGTAAGAGCTTACTGAAAAAATTAACATCTCTTGCTAAGCTTATTAAAGGCTTATAACACCTTCAGGCGGCCAGTCCGCCTg                                                                                              <  2:177218/100‑1 (MQ=255)
       gcgTGTTTACAACAAGCTGTAAGAGCTTACTGAAAAAATTAACATCTCTTGCTAAGCTTATTAAAGGCTTATAACACCATCAGGCGGACTGGCCGCCTGa                                                                                             <  1:2642846/100‑1 (MQ=255)
       gcatgtTTACAACAAGCTGTAAGAGCTTACTGAAAAAATTAACATCTCTTGCTAAGCTTATTAAAGGCTTATAACACCTTCAGGCGGACTGGCCGCCTGa                                                                                             <  2:298311/97‑1 (MQ=255)
        cgTGTTTACAACAAGCTGTAAGAGCTTACTGAAAAAATTAACATCTCTTGCTAAGCTTATTAAAGGCTTATAACACCTTCAGGCGGCCAGTCCGCCTGAt                                                                                            <  2:3877102/100‑1 (MQ=255)
        cgTGTTTACAACAAGCTGTAAGAGCTTACTGAAAAAATTAACATCTCTTGCTAAGCTTATTAAAGGCTTATAACACCTTCAGGCGGACTGGCCGCCTGaa                                                                                            <  1:2894903/100‑2 (MQ=255)
         gtgtTTACAACAAGCTGTAAGAGCTTACTGAAAAAATTAACATCTCTTGCTAAGCTTATTAAAGGCTTATAACACCTTCAGGCGGCCAGTCCGCCTGAtt                                                                                           <  2:2661049/100‑1 (MQ=255)
         gtgtTTACAACAAGCTGTAAGAGCTTACTGAAAAAATTAACATCTCTTGCTAAGCTTATTAAAGGCTTATAACACCTTCAGGCGGACTGGCCGCCTGaag                                                                                           <  1:3392628/100‑3 (MQ=255)
          tgtTTACAACCAGCTGTAAGAGCTTACTGACAAAAGTAACATCTCTTGCTAAGCTTATTAAAGGCTTATAACACCTTCAGGCGGACTGGCCGCCTGaagg                                                                                          <  2:335118/100‑4 (MQ=255)
          tgtTTACAACAAGCTGTAAGAGCTTACTGAAAAAATTAACATCTCTTGCTAAGCTTATTAAAGGCTTATAACACCTTCAGGCGGCCAGTCCGCCTGAttt                                                                                          >  1:822765/1‑100 (MQ=255)
          tgtTTACAACAAGCTGTAAGAGCTTACTGAAAAAATTAACATCTCTTGCTAAGCTTATTAAAGGCTTATAACACCTTCAGGCGGACTGGCCGCCTGaagg                                                                                          <  2:196513/100‑4 (MQ=255)
           gtTTACAACAAGCTGTAAGAGCTTACTGAAAAAATTAACATCTCTTGCTAAGCTTATTAAAGGCTTATAACACCTTCAGGCGGCCAGTCCGCCTGATTTc                                                                                         >  2:855553/1‑100 (MQ=255)
           gtTTACAACAAGCTGTAAGAGCTTACTGAAAAAATTAACATCTCTTGCTAAGCTTATTAAAGGCTTATAACACCTTCAGGCGGCCAGTCCGCCTGATTTc                                                                                         <  2:3068652/100‑1 (MQ=255)
           gtTTACAACAAGCTGTAAGAGCTTACTGAAAAAATTAACATCTCTTGCTAAGCTTATTAAAGGCTTATAACACCTTCAGGCGGACTGGCCGCCTGaagg                                                                                          <  2:3536951/99‑4 (MQ=255)
               acaacaAGCTGTAAGAGCTTACTGAAAAAATTAACATCTCTTGCTAAGCTTATTAAAGGCTTATAACACCTTCAGGCGGCCAGTCCGCCTGATTTCAttt                                                                                     <  2:3754527/100‑1 (MQ=255)
               acaacaAGCTGTAAGAGCTTACTGAAAAAATTAACATCTCTTGCTAAGCTTATTAAAGGCTTATAACACCTTCAGGCGGCCAGTCCGCCTGATTTCAttt                                                                                     <  1:482649/100‑1 (MQ=255)
                caacaaGCTGTAAGAGCTTACTGAAAAAATTAACATCTCTTGCTAAGCTTATTAAAGGCTTATAACACCTTCAGGCGGCCAGTCCGCCTGATTTCAtttt                                                                                    >  1:235651/1‑100 (MQ=255)
                 aacaaGCTGTAAGAGCTTACTGAAAAAATTAACATCTCTTGCTAAGCTTATTAAAGGCTTATAACACCTTCAGGCGGCCAGTCCGCCTGATTTCATTTTa                                                                                   <  1:2678540/100‑1 (MQ=255)
                 aacaaGCTGTAAGAGCTTACTGAAAAAATTAACATCTCTTGCTAAGCTTATTAAAGGCTTATAACACCTTCAGGCGGCCAGTCCGCCTGATTTCATTTTa                                                                                   >  1:3981143/1‑100 (MQ=255)
                  acaaGCTGTAAGAGCTTACTGAAAAAATTCACATATCTTGCTAAGCTTATTAAAGGCTTATAACACCTTCAGGCGGCCAGTCCGCCTGCTATCCtttc                                                                                    >  1:1393569/1‑97 (MQ=255)
                  acaaGCTGTAAGAGCTTACTGAAAAAATTAACATCTCTTGCTAAGCTTATTAAAGGCTTATAACACCTTCAGGCGGCCAGTCCGCCTGATTTCATTTTAt                                                                                  >  2:184015/1‑100 (MQ=255)
                    aaGCTGTAAGAGCTTACTGAAAAAATTAACATCTCTTGCTAAGCTTATTAAAGGCTTATAACACCTTCAGGCGGCCAGTCCGCCTGATTTCATTTTATgg                                                                                <  2:1595630/100‑1 (MQ=255)
                     aGCTGTAAGAGCTTACTGAAAAAATTAACATCTCTTGCTAAGCTTATTAAAGGCTTATAACACCTTCAGGCGGCCAGTCCGCCTGATTTCATTTTATGGa                                                                               >  1:330500/1‑100 (MQ=255)
                      gCTGAAAGAGCTTACCGAAAAAATTAACATCTCTTTCTAAGCTTATTAAAGGCTTATAACACCTTCAGGCGGCCAGTCCGCCTGATTTCATTATATgg                                                                                >  2:631398/1‑98 (MQ=255)
                         gTAAGAGCTTACTGAAAAAATTAACATCTCTTGCTAAGCTTATTAAAGGCTTATAACACCTTCAGGCGGCCAGTCCGCCTGATTTCATTTTATGGATAAt                                                                           <  2:1277658/100‑1 (MQ=255)
                              agCTTACTGAAAAAATTAACATCTCTTGCTAAGCTTATTAAAGGCTTATAACACCTTCAGGCGGCCAGTCCGCCTGATTTCATTTTATGGATAATCATTa                                                                      <  2:330500/100‑1 (MQ=255)
                                cTTACTGAAAAAATTAACATCTCTTGCTAAGCTTATTAAAGGCTTATAACACCTTCAGGCGGCCAGTCCGCCTGATTTCATTTTATGGATAATCATTATg                                                                    <  2:35810/100‑1 (MQ=255)
                                   aCTGAAAAAATTAACATCTCTTGCTAAGCTTATTAAAGGCTTATAACACCTTCAGGCGGCCAGTCCGCCTGATTTCATTTTATGGATAATCATTATGAAt                                                                 >  1:3423844/1‑100 (MQ=255)
                                     tGAAAAAATTAACATCTCTTGCTAAGCTTATTAAAGGCTTATAACACCTTCAGGCGGCCAGTCCGCCTGATTTCATTTTATGGATAATCATTATGAATaa                                                               >  1:3017254/1‑100 (MQ=255)
                                      gAAAAAATTAACATCTCTTGCTAAGCTTATTAAAGGCTTATAACACCTTCAGGCGGCCAGTCCGCCTGATTTCATTTTATGGATAATCATTATGAATaaa                                                              >  2:2344729/1‑100 (MQ=255)
                                      gAAAAAATTAACATCTCTTGCTAAGCTTATTAAAGGCTTATAACACCTTCAGGCGGCCAGTCCGCCTGATTTCATTTTATGGATAATCATTATGAATaaa                                                              >  1:3025830/1‑100 (MQ=255)
                                      gAAAAAATTAACATCTCTTGCTAAGCTTATTAAAGGCTTATAACACCTTCAGGCGGCCAGTCCGCCTGATTTCATTTTATGGATAATCATTATGAATaaa                                                              >  2:57851/1‑100 (MQ=255)
                                        aaaaaTTAACATCTCTTGCTAAGCTTATTAAAGGCTTATAACACCTTCAGGCGGCCAGTCCGCCTGATTTCATTTTATGGATAATCATTATGAATAAATc                                                            <  1:3422493/100‑1 (MQ=255)
                                        aaaaaTTAACATCTCTTGCTAAGCTTATTAAAGGCTTATAACACCTTCAGGCGGCCAGTCCGCCTGATTTCATTTTATGGATAATCATTATGAATAAATc                                                            <  1:835327/100‑1 (MQ=255)
                                           aaTTAACATCTCTTGCTAAGCTTATTAAAGGCTTATAACACCTTCAGGCGGCCAGTCCGCCTGATTTCATTTTATGGATAATCATTATGAATAAATCGCt                                                         >  1:1174214/1‑100 (MQ=255)
                                             ttAACATCTCTTGCTAAGCTTATTAAAGGCTTATAACACCTTCAGGCGGCCAGTCCGCCTGATTTCATTTTATGGATAATCATTATGAATAAATCGCTgg                                                       <  1:1914653/100‑1 (MQ=255)
                                                 cATCTCTTGCTAAGCTTATTAAAGGCTTATAACACCTTCAGGCGGCCAGTCCGCCTGATTTCATTTTATGGATAATCATTATGAATAAATCGCTGGTAGc                                                   <  2:2036061/100‑1 (MQ=255)
                                                 cATCTCTTGCTAAGCTTATTAAAGGCTTATAACACCTTCAGGCGGCCAGTCCGCCTGATTTCATTTTATGGATAATCATTATGAATAAATCGCTGGTAGc                                                   <  2:1119502/100‑1 (MQ=255)
                                                  aTCTCTTGCTAAGCTTATTAAAGGCTTATAACACCTTCAGGCGGCCAGTCCGCCTGATTTCATTTTATGGATAATCATTATGAATAAATCGCTGGTAGCg                                                  >  2:643067/1‑100 (MQ=255)
                                                  aTCTCTTGCTAAGCTTATTAAAGGCTTATAACACCTTCAGGCGGCCAGTCCGCCTGATTTCATTTTATGGATAATCATTATGAATAAATCGCTGGTAGCg                                                  >  2:2462631/1‑100 (MQ=255)
                                                   tctcTTGCTAAGCTTATTAAAGGCTTATAACACCTTCAGGCGGCCAGTCCGCCTGATTTCATTTTATGGATAATCATTATGAATAAATCGCTGGTAGCgg                                                 <  1:4021589/100‑1 (MQ=255)
                                                    ctctTGCTAAGCTTATTAAAGGCTTATAACACCTTCAGGCGGCCAGTCCGCCTGATTTCATTTTATGGATAATCATTATGAATAAATCGCTGGTAg                                                    >  2:2797101/1‑96 (MQ=255)
                                                      ctTGCTAAGCTTATTAAAGGCTTATAACACCTTCAGGCGGCCAGTCCGCCTGATTTCATTTTATGGATAATCATTATGAATAAATCGCTGGTAGCGGTAg                                              <  1:1912068/100‑1 (MQ=255)
                                                          cTAAGCTTATTAAAGGCTTATAACACCTTCAGGCGGCCAGTCCGCCTGATTTCATTTTATGGATAATCATTATGAATAAATCGCTGGTAGCGGTAGGCGt                                          >  2:91946/1‑100 (MQ=255)
                                                          cTAAGCTTATTAAAGGCTTATAACACCTTCAGGCGGCCAGTCCGCCTGATTTCATTTTATGGATAATCATTATGAATAAATCGCTGGTAGCGGTAGGCGt                                          <  1:4079408/100‑1 (MQ=255)
                                                           tAAGCTTATTAAAGGCTTATAACACCTTCAGGCGGCCAGTCCGCCTGATTTCATTTTATGGATAATCATTATGAATAAATCGCTGGTAGCGGTAGGCGTc                                         >  1:4053170/1‑100 (MQ=255)
                                                             aGCTTATTAAAGGCTTATAACACCTTCAGGCGGCCAGTCCGCCTGATTTCATTTTATGGATAATCATTATGAATAAATCGCTGGTAGCGGTAggagatcg                                       >  2:3998776/1‑94 (MQ=255)
                                                             aGCTTATTAAAGGCTTATAACACCTTCAGGCGGCCAGTCCGCCTGATTTCATTTTATGGATAATCATTATGAATAAATCGCTGGTAGCGGTAGGCGTCAt                                       >  1:1086773/1‑100 (MQ=255)
                                                              gCTTATTAAAGGCTTATAACACCTTCAGGCGGCCAGTCCGCCTGATTTCATTTTATGGATAATCATTATGAATAAATCGCTGGTAGCGGTAGGCGTCAtt                                      >  2:2351687/1‑100 (MQ=255)
                                                              gCTTATTAAAGGCTTATAACACCTTCAGGCGGCCAGTCCGCCTGATTTCATTTTATGGATAATCATTATGAATAAATCGCTGGTAGCGGTAGGCGTCAtt                                      >  1:3533913/1‑100 (MQ=255)
                                                               cTTATTAAAGGCTTATAACACCTTCAGGCGGCCAGTCCGCCTGATTTCATTTTATGGATAATCATTATGAATAAATCGCTGGTAGCGGTAGGCGTCAttg                                     >  1:2481602/1‑100 (MQ=255)
                                                               cTTATTAAAGGCTTATAACACCTTCAGGCGGCCAGTCCGCCTGATTTCATTTTATGGATAATCATTATGAATAAATCGCTGGTAGCGGTAGGCGTCAttg                                     >  2:445084/1‑100 (MQ=255)
                                                                  attaAAGGCTTATAACACCTTCAGGCGGCCAGTCCGCCTGATTTCATTTTATGGATAATCATTATGAATAAATCGCTGGTAGCGGTAGGCGTCAttgttg                                  <  2:1229427/100‑1 (MQ=255)
                                                                   ttaAAGGCTTATAACACCTTCAGGCGGCCAGTCCGCCTGATTTCATTTTATGGATAATCATTATGAATAAATCGCTGGTAGCGGTAGGCGTCATTGTTgc                                 >  1:2017882/1‑100 (MQ=255)
                                                                   ttaAAGGCTTATAACACCTTCAGGCGGCCAGTCCGCCTGATTTCATTTTATGGATAATCATTATGAATAAATCGCTGGTAGCGGTAGGCGTCATTGTTgc                                 <  2:409306/100‑1 (MQ=255)
                                                                    taAAGGCTTATAACACCTTCAGGCGGCCAGTCCGCCTGATTTCATTTTATGGATAATCATTATGAATAAATCGCTGGTAGCGGTAGGCGTCATTGTTgcg                                <  1:1627165/100‑1 (MQ=255)
                                                                    taAAGGCTTATAACACCTTCAGGCGGCCAGTCCGCCTGATTTCATTTTATGGATAATCATTATGAATAAATCGCTGGTAGCGGTAGGCGTCATTGTTgcg                                <  1:1395988/100‑1 (MQ=255)
                                                                      aaGGCTTATAACACCTTCAGGCGGCCAGTCCGCCTGATTTCATTTTATGGATAATCATTATGAATAAATCGCTGGTAGCGGTAGGCGTCATTGTTGCGCt                              <  1:489319/100‑1 (MQ=255)
                                                                        ggCTTATAACACCTTCAGGCGGCCAGTCCGCCTGATTTCATTTTATGGATAATCATTATGAATAAATCGCTGGTAGCGGTAGGCGTCATTGTTGCGCTa                             >  1:1035023/1‑99 (MQ=255)
                                                                         gCTTATAACACCTTCAGGCGGCCAGTCCGCCTGATTTCATTTTATGGATAATCATTATGAATAAATCGCTGGTAGCGGTAGGCGTCATTGTTGCGCTAgg                           <  2:1529537/100‑1 (MQ=255)
                                                                          cTTATAACACCTTCAGGCGGCCAGTCCGCCTGATTTCATTTTATGGATAATCATTATGAATAAATCGCTGGTAGCGGTAGGCGTCATTGTTGCGCTAGGc                          >  2:754427/1‑100 (MQ=255)
                                                                           ttATAACACCTTCAGGCGGCCAGTCCGCCTGATTTCATTTTATGGATAATCATTATGAATAAATCGCTGGTAGCGGTAGGCGTCATTGTTGCGCTAGGCg                         <  2:3806613/100‑1 (MQ=255)
                                                                            tataACACCTTCAGGCGGCCAGTCCGCCTGATTTCATTTTATGGATAATCATTATGAATAAATCGCTGGTAGCGGTAGGCGTCATTGTTGCGCTAGGCGt                        >  2:1438751/1‑100 (MQ=255)
                                                                            tataACACCTTCAGGCGGCCAGTCCGCCTGATTTCATTTTATGGATAATCATTATGAATAAATCGCTGGTAGCGGTAGGCGTCATTGTTGCGCTAGGCGt                        <  2:206132/100‑1 (MQ=255)
                                                                            taaaatgaaatCAGGCGGACTGGCCGCCTGATTTCATTTTATGGATAATCATTATGAATAAATCGCTGGTAGCGGTAGGCGTCATTGTTGCGCTAGGCGt                        >  1:706409/11‑100 (MQ=37)
                                                                              taACACCTTCAGGCGGCCAGTCCGCCTGATTTCATTTTATGGATAATCATTATGGAAAAAACGCTGGTAGCAGTAGACGTCATTGTTGCGCTAGGCGTa                       >  2:2496050/1‑99 (MQ=255)
                                                                               aaCACCTTCAGGCGGCCAGTCCGCCTGATTTCATTTTATGGATAATCATTATGAATAAATCGCTGGTAGCGGTAGGCGTCATTGTTGCGCTAGGCGTAGt                     <  1:3002055/100‑1 (MQ=255)
                                                                                acacCTTCAGGCGGCCAGTCCGCCTGATTTCATTTTATGGATAATCATTATGAATAAATCGCTGGTAGCGGTAGGCGTCATTGTTGCGCTAGGCGTAGTc                    >  2:3225693/1‑100 (MQ=255)
                                                                                acacCTTCAGGCGGCCAGTCCGCCTGATTTCATTTTATGGATAATCATTATGAATAAATCGCTGGTAGCGGTAGGCGTCATTGTTGCGCTAGGCGTAGTc                    >  2:2296156/1‑100 (MQ=255)
                                                                                acacCTTCAGGCGGCCAGTCCGCCTGATTTCATTTTATGGATAATCATTATGAATAAATCGCTGGTAGCGGTAGGCGTCATTGTTGCGCTAGGCGTAGTc                    >  2:2707387/1‑100 (MQ=255)
                                                                                  gaaatCAGGCGGACTGGCCGCCTGATTTCATTTTATGGATAATCATTATGAATAAATCGCTGGTAGCGGTAGGCGTCATTGTTGCGCTAGGCGTAGTCTg                  >  2:602303/5‑100 (MQ=255)
                                                                                  gaaatCAGGCGGACTGGCCGCCTGATTTCATTTTATGGATAATCATTATGAATAAATCGCTGGTAGCGGTAGGCGTCATTGTTGCGCTAGGCGTAGTCTg                  >  1:356407/5‑100 (MQ=255)
                                                                                  acCTTCAGGCGGCCAGTCCGCCTGATTTCATTTTATGGATAATCATTATGAATAAATCGCTGGTAGCGGTAGGCGTCATTGTTGCGCTAGGCGTAGTCTg                  >  1:2394190/1‑100 (MQ=255)
                                                                                   ccTTCAGGCGGCCAGTCCGCCTGATTTCATTTTATGGATAATCATTATGAATAAATCGCTGGTAGCGGTAGGCGTCATTGTTGCGCTAGGCGTAGTCtcg                 >  2:2178564/1‑98 (MQ=255)
                                                                                   ccTTCAGGCGGCCAGTCCGCCTGATTTCATTTTATGGATAATCATTATGAATAAATCGCTGGTAGCGGTAGGCGTCATTGTTGCGCTAGGCGTAGTCTgg                 <  2:1590956/100‑1 (MQ=255)
                                                                                   ccTTCAGGCGGCCAGTCCGCCTGATTTCATTTTATGGATAATCATTATGAATAAATCGCTGGTAGCGGTAGGCGTCATTGTTGCGCTAGGCGTAGTCTgg                 >  1:4105581/1‑100 (MQ=255)
                                                                                   aaatCAGGCGGACTGGCCGCCTGATTTCATTTTATGGATAATCATTATGAATAAATCGCTGGTAGCGGTAGGCGTCATTGTTGCGCTAGGCGTAGTCTgg                 >  2:4081456/4‑100 (MQ=255)
                                                                                   aaatCAGGCGGACTGGCCGCCTGATTTCATTTTATGGATAATCATTATGAATAAATCGCTGGTAGCGGTAGGCGTCATTGTTGCGCTAGGCGTAGTCTgg                 >  1:3253555/4‑100 (MQ=255)
                                                                                    cTTCAGGCGGCCAGTCCGCCTGATTTCATTTTATGGATAATCATTATGAATAAATCGCTGGTAGCGGTAGGCGTCATTGTTGCGCTAGGCGTAGTCTGGa                >  1:2060769/1‑100 (MQ=255)
                                                                                    aatCAGGCGGACTGGCCGCCTGATTTCATTTTATGGATAATCATTATGAATAAATCGCTGGTAGCGGTAGGCGTCATTGTTGCGCTAGGCGTAGTCTGGa                >  2:576283/3‑100 (MQ=255)
                                                                                    aatCAGGCGGACTGGCCGCCTGATTTCATTTTATGGATAATCATTATGAATAAATCGCTGGTAGCGGTAGGCGTCATTGTTGCGCTAGGCGTAGTCTGGa                >  1:525588/3‑100 (MQ=255)
                                                                                    aatCAGGCGGACTGGCCGCCTGATTTCATTTTATGGATAATCATTATGAATAAATCGCTGGTAGCGGTAGGCGTCATTGTTGCGCTAGGCGTAGTCTGGa                >  1:1472652/3‑100 (MQ=255)
                                                                                    aatCAGGCGGACTGGCCGCCTGATTTCATTTTATGGATAATCATTATGAATAAATCGCTGGTAGCGGTAGGCGTCATTGTTGCGCTAGGCGTAGTCTGGa                >  1:1522191/3‑100 (MQ=255)
                                                                                    aatCAGGCGGACTGGCCGCCTGATTTCATTTTATGGATAATCATTATGAATAAATCGCTGGTAGCGGTAGGCGTCATTGTTGCGCTAGGCGTAGTCTGGa                >  1:857989/3‑100 (MQ=255)
                                                                                     ttCAGGCGGCCAGTCCGCCTGATTTCATTTTATGGATAATCATTATGAATAAATCGCTGGTAGCGGTAGGCGTCATTGTTGCGCTAGGCGTAGTCTGGAc               <  2:3017241/100‑1 (MQ=255)
                                                                                     ttCAGGCGGCCAGTCCGCCTGATTTCATTTTATGGATAATCATTATGAATAAATCGCTGGTAGCGGTAGGCGTCATTGTTGCGCTAGGCGTAGTCTGGAc               <  1:1083609/100‑1 (MQ=255)
                                                                                     ttCAGGCGGCCAGTCCGCCTGATTTCATTTTATGGATAATCATTATGAATAAATCGCTGGTAGCGGTAGGCGTCATTGTTGCGCTAGGCGTAGTCTGGAc               <  1:1221904/100‑1 (MQ=255)
                                                                                     atCAGGCGGACTGGCCGCCTGATTTCATTTTATGGATAATCATTATGACTAAATCGCTGGTATCGGTAGGCGTCATTGTTGCGCTAGGCGTAGTCTg                  >  2:378771/2‑97 (MQ=255)
                                                                                     atCAGGCGGACTGGCCGCCTGATTTCATTTTATGGATAATCATTATGAATAACTCGCTGGTAGCGGTAGGCGTCATTGTTGCGCTAGGCGTAGTCTGGAc               >  2:2673432/2‑100 (MQ=255)
                                                                                          gcggcCAGTCCGCCTGATTTCATTTTATGGATAATCATTATGAATAAATCGCTGGTAGCGGTAGGCGTCATTGTTGCGCTAGGCGTAGTCTGGACAggcg          <  1:2935313/100‑1 (MQ=255)
                                                                                            ggcCAGTCCGCCTGATTTCATTTTATGGATAATCATTATGAATAAATCGCTGGTAGCGGTAGGCGTCATTGTTGCGCTAGGCGTAGTCTGGACAggcggc        <  1:1002399/100‑1 (MQ=255)
                                                                                            ggcCAGTCCGCCTGATTTCATTTTATGGATAATCATTATGAATAAATCGCTGGTAGCGGTAGGCGTCATTGTTGCGCTAGGCGTAGTCTGGACAggcggc        >  1:1702381/1‑100 (MQ=255)
                                                                                            ggcCAGTCCGCCTGATTTCATTTTATGGATAATCATTATGAATAAATCGCTGGTAGCGGTAGGCGTCATTGTTGCGCTAGGCGTAGTCTGGACAggcggc        >  1:482181/1‑100 (MQ=255)
                                                                                               cAGTCCGCCTGATTTCATTTTATGGATAATCATTATGAATAAATCGCTGGTAGCGGTAGGCGTCATTGTTGCGCTAGGCGTAGTCTGGACAGGCGGCGCa     <  1:3876961/100‑1 (MQ=255)
                                                                                                  tCCGCCTGATTTCATTTTATGGATAATCATTATGAATAAATCGCTGGTAGCGGTAGGCGTCATTGTTGCGCTAGGCGTAGTCTGGACAGGCGGCGCATgg  <  2:2805684/100‑1 (MQ=255)
                                                                                                  tCCGCCTGATTTCATTTTATGGATAATCATTATGAATAAATCGCTGGTAGCGGTAGGCGTCATTGTTGCGCTAGGCGTAGTCTGGACAGGCGGCGCATgg  <  1:2575489/100‑1 (MQ=255)
                                                                                                  |                                                                                                   
GTTTAGCGCGTGTTTACAACAAGCTGTAAGAGCTTACTGAAAAAATTAACATCTCTTGCTAAGCTTATTAAAGGCTTATAACACCTTCAGGCGGCCAGTCCGCCTGATTTCATTTTATGGATAATCATTATGAATAAATCGCTGGTAGCGGTAGGCGTCATTGTTGCGCTAGGCGTAGTCTGGACAGGCGGCGCATGG  >  NC_000913/1689723‑1689920

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: