BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	1,756,529	A→T	100%	T278S (ACC→TCC)	pykF →	pyruvate kinase I

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,756,529	0	A	T	97.2%	354.7 / ‑0.5	105	T278S (ACC→TCC)	pykF	pyruvate kinase I
Reads supporting (aligned to +/- strand): ref base A (1/2); new base T (46/56); total (47/58)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.73e-01

Only 100 of 105 total aligned reads displayed.

GACCTGGGTGTAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGACGCAGT  >  NC_000913/1756433‑1756623

gACCTGGGTGTAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGTccc                                                                                             <  2:2229257/100‑1 (MQ=255)

gACCTGGGTGTAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGTccc                                                                                             <  2:1400215/100‑1 (MQ=255)

 aCCTGGGTGTAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGTCCCa                                                                                            <  1:1723671/100‑1 (MQ=255)

 aCCTGGGTGTAGAAAACCCGATAGAAGAAGTTATCTTCTCCCAGAAGAAAAAAAACAAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGTcc                                                                                              <  2:2638846/98‑1 (MQ=255)

  ccTGGGTGTAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGTCCCAg                                                                                           >  1:1325315/1‑100 (MQ=255)

  ccTGGGTGTAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGTCCCAg                                                                                           >  1:1636201/1‑100 (MQ=255)

  ccTGGGTGTAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGTCCCAg                                                                                           >  1:3883631/1‑100 (MQ=255)

    tGGGTGTAGAAATCCCGGTAGAAGAAGTTATCTACGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCGCGTAAAGTCGTTATCACTGCGTCCCAGAt                                                                                         >  2:2518326/1‑100 (MQ=255)

      ggTGTAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGTCCCAGAt                                                                                         <  2:1012215/98‑1 (MQ=255)

       gtgtAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGTCCCAGATGCt                                                                                      <  2:1785987/100‑1 (MQ=255)

       gtgtAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGTCCCAGATGCt                                                                                      >  1:536716/1‑100 (MQ=255)

        tgtAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGTCCCAGATGCTg                                                                                     <  1:387125/100‑1 (MQ=255)

         gtAGAAATCCCGGTAGAAGAAGTTATTTTCGCCCAGAAGAGGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGTCCCAGATGCTgg                                                                                    <  2:3668248/100‑1 (MQ=255)

         gtAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGTCCCAGATGCTgg                                                                                    <  1:1181796/100‑1 (MQ=255)

         gtAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGTCCCAGATGCTgg                                                                                    >  2:2887407/1‑100 (MQ=255)

           aGAAATCCCGGTATAATAAGTTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGTCCCAGATGCTGGAt                                                                                  <  1:2441093/100‑1 (MQ=255)

           aGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGTCCCAGATGCTGGAt                                                                                  >  2:3083689/1‑100 (MQ=255)

           aGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGTCCCAGATGCTGGAt                                                                                  >  1:1475252/1‑100 (MQ=255)

           aGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATACGTGCACGTAAAGTCGTTATCACTGCGTCCCAGATACTGGa                                                                                   >  2:1045645/1‑99 (MQ=255)

            gAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGTCCCAGATGCTGGAtt                                                                                 >  1:681033/1‑100 (MQ=255)

                 cccGGTAGAAGAAGTTATCTTCGCCCCGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGTCCCAGATTCTGGATTCCATg                                                                            >  1:2555265/1‑100 (MQ=255)

                  ccGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGTCCCAGATGCTGGATTCCATGa                                                                           >  1:3308843/1‑100 (MQ=255)

                   cGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGTCCCAGATGCTGGATTCCATGa                                                                           <  2:3874834/99‑1 (MQ=255)

                    ggTAGAAGAAGTTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGTCCCAGATGCTGGATTCCATGATc                                                                         >  2:3836768/1‑100 (MQ=255)

                    ggTAGAAGAAGTTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGTCCCAGATGCTGGATTCCATGATc                                                                         <  1:2287560/100‑1 (MQ=255)

                    ggTAGAAGAAGTTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGTCCCAGATGCTGGATTCCATGATc                                                                         <  1:523558/100‑1 (MQ=255)

                     gTAGAAGAAGTTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGTCCCAGATGCTGGATTCCATGATCa                                                                        <  1:2552121/100‑1 (MQ=255)

                     gTAGAAGAAGTTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGTCCCAGATGCTGGATTCCATGATCa                                                                        <  2:1429904/100‑1 (MQ=255)

                     gTAGAAGAAGTTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGTCCCAGATGCTGCATTCCATGATCa                                                                        <  1:2763315/100‑1 (MQ=255)

                      tAGAAGAAGTTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGTCCCAGATGCTGGATTCCATGATCaa                                                                       <  1:2669828/100‑1 (MQ=255)

                       agaagaAGTTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGTCCCAGATGCTGGATTCCATGATCaaa                                                                      <  2:3668447/100‑1 (MQ=255)

                       agaagaAGTTATCTTCGCACAGAAAAAAAAAAAAAAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGTCCCAGATGCTGGATTCCATGAt                                                                          <  2:2384267/96‑1 (MQ=255)

                          agaagTTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGTCCCAGATGCTGGATTCCATGATCAAAAAc                                                                   <  1:4133129/100‑1 (MQ=255)

                          agaagGTATCTTCGCCCAGAAGATGATGATTGAAAAATGTATCCGTGCACGTAAAGTCGTTATCTCTGCGTCCCAGATGCTGGATTCCATGATCAAAAAc                                                                   <  1:3749214/100‑1 (MQ=255)

                             agTTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGTCCCAGATGCTGGATTCCATGATCAAAAACCCa                                                                <  1:3725331/100‑1 (MQ=255)

                              gTTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGTCCCAGATGCTGGATTCCATGATCAAAAACCCAc                                                               >  2:2263656/1‑100 (MQ=255)

                              gTTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGTCCCAGATGCTGGATTCCATGATCAAAAACCCAc                                                               >  2:2903089/1‑100 (MQ=255)

                                 aTCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGTCCCAGATGCTGGATTCCATGATCAAAAACCCACGcc                                                            <  2:4034584/100‑1 (MQ=255)

                                 aTCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCAAAAACCCACGcc                                                            >  1:1888984/1‑100 (MQ=255)

                                  tCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGTCCCAGATGCTGGATTCCATGATCAAAAACCCACGccc                                                           >  1:2753238/1‑100 (MQ=255)

                                   cTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGTCCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCg                                                          <  1:2509088/100‑1 (MQ=255)

                                   cTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGTCCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCg                                                          >  1:991533/1‑100 (MQ=255)

                                   cTTCGCCCAAAAGATGATGAGCAAAAAATGTATCCGTGCACGTAAAGTCATTATCACTACGTCCCAGATGCTGGATTCCATGATCAAAAACCCACGcc                                                            <  2:366363/98‑1 (MQ=255)

                                       gCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGTCCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTc                                                      >  1:675582/1‑100 (MQ=255)

                                          cAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGTCCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTcgcg                                                   >  1:3544008/1‑100 (MQ=255)

                                           agaagaTGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGTCCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCgcgc                                                  <  1:2356769/100‑1 (MQ=255)

                                           agaagaTGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGTCCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCgcgc                                                  <  1:1215098/100‑1 (MQ=255)

                                            gaagaTGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGTCCCAGATGCTGGATTCCATGCTCAAAAACCCACGCCCGACTCGCGCa                                                 >  2:295271/1‑100 (MQ=255)

                                             aagaTGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGTCCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAg                                                <  2:3883606/100‑1 (MQ=255)

                                             aagaTGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGTCCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAg                                                >  1:1200742/1‑100 (MQ=255)

                                                atgatgatCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGTCCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAg                                             <  2:1263643/100‑1 (MQ=255)

                                                  gatgatCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGTCCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGcc                                           <  2:2849333/100‑1 (MQ=255)

                                                   atgatCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGTCCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCg                                          >  1:2319044/1‑100 (MQ=255)

                                                     gatCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGTCCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGt                                        <  2:130290/100‑1 (MQ=255)

                                                      atCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGTCCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTg                                       <  1:2413806/100‑1 (MQ=255)

                                                       tCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGTCCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGa                                      >  2:56553/1‑100 (MQ=255)

                                                        cGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGTCCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTg                                       >  2:1620882/1‑98 (MQ=255)

                                                         gAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGTCCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACg                                    >  1:3195791/1‑100 (MQ=255)

                                                         gAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGTCCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACg                                    >  1:1960651/1‑100 (MQ=255)

                                                          aaaaaTGTATCCGTGCACGTAAAGTCGTTATCACTGCGTCCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGt                                   >  2:2024023/1‑100 (MQ=255)

                                                            aaaTGTATCCGTGCACGTAAAGTCGTTATCACTGCGTCCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTg                                 <  1:828443/100‑1 (MQ=255)

                                                            aaaTGTATCCGTGCACGTAAAGTCGTTATCACTGCGTCCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTg                                 <  1:65915/100‑1 (MQ=255)

                                                             aaTGTATCCGTGCACGTAAAGTCGTTATCACTGCGTCCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGc                                <  1:1471467/100‑1 (MQ=255)

                                                             aaTGTATCCGTGCACGTAAAGTCGTTATCACTGCGTCCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGc                                <  1:2887416/100‑1 (MQ=255)

                                                             aaTGTATCCGTGCACGTAAAGTCGTTATCACTGCGTCCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGc                                >  1:751114/1‑100 (MQ=255)

                                                             aaTGTATCCGTGCACGTAAAGTCGTTATCACTGCGTCCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGc                                >  1:3387297/1‑100 (MQ=255)

                                                               tGTATCCGTGCACGTAAAGTCGTTATCACTGCGTCCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCaa                              >  1:274910/1‑100 (MQ=255)

                                                                 tATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAAc                            <  2:1888981/100‑1 (MQ=255)

                                                                  aTCCGTGCACGTAAAGTCGTTATCACTGCGTCCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACg                           <  2:3263164/100‑1 (MQ=255)

                                                                  aTCCGTGCACGTAAAGTCGTTATCACTGCGTCCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACg                           <  2:2460212/100‑1 (MQ=255)

                                                                   tCCGTGCACGTAAAGTCGTTATCACTGCGTCCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGc                          <  1:1344925/100‑1 (MQ=255)

                                                                   tCCGTGCACGTAAAGTCGTTATCACTGCGTCCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGc                          <  1:1348189/100‑1 (MQ=255)

                                                                    ccGTGCACGTAAAGTCGTTATCACTGCGTCCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGcc                         <  1:3096881/100‑1 (MQ=255)

                                                                        gCACGTAAAGTCGTTATCACTGCGTCCCAGATGCTGGATTCCATGATCAAAAACCCGCGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATcc                     >  2:2680934/1‑100 (MQ=255)

                                                                        gCACGTAAAGTCGTTATCACTGCGTCCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATcc                     >  1:1833957/1‑100 (MQ=255)

                                                                           cctAAAGTCGTTATCACTGCGTCCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCg                  <  2:2534621/98‑1 (MQ=255)

                                                                           catAAAGTCGTTATCACTGCGTCCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCg                  >  1:2846938/3‑100 (MQ=255)

                                                                            gTAAAGTCGTTATCACTGCGTCCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCg                  >  1:1159852/1‑99 (MQ=255)

                                                                             tAAAGTCGTTATCACTGCGTCCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGAc                <  1:864556/100‑1 (MQ=255)

                                                                             tAAAGTCGTTATCACTGCGTCCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGAc                >  1:4132074/1‑100 (MQ=255)

                                                                               aaGTCGTTATCACTGCGTCCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACgg              <  1:176303/100‑1 (MQ=255)

                                                                                aGTCGTTATCACTGCGTCCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGt             <  2:3419303/100‑1 (MQ=255)

                                                                                aGTCGTTATCACTGCGTCCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGt             >  1:3121416/1‑100 (MQ=255)

                                                                                 gTCGTTATCACTGCGTCCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGGCTCGCGCAGACGCCGGTGACGTTGCAAACGCCATCCTCGACGGTa            >  2:3297127/1‑100 (MQ=255)

                                                                                 gTCGTTATCACTGCGTCCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGt             >  2:1190511/1‑99 (MQ=255)

                                                                                  tCGTTATCACTGCGTCCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTAc           <  2:1454365/100‑1 (MQ=255)

                                                                                  tCGTTATCACTGCGTCCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTAc           <  1:3083702/100‑1 (MQ=255)

                                                                                  tCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTAc           <  1:3165390/100‑1 (MQ=255)

                                                                                  tCGATATCACTGCGTCCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTAc           <  2:4157769/100‑1 (MQ=255)

                                                                                   cGTTATCACTGCGTCCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACt          >  1:3786524/1‑100 (MQ=255)

                                                                                    gTTATCACTGCGTCCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGAGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTg         >  2:2686713/1‑100 (MQ=255)

                                                                                    gTTATCACTGCGTCCCAGATGCTGGAAAAAAAAAAAAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGAc                <  2:3035561/93‑1 (MQ=255)

                                                                                       aTCACTGCGTCCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGACg      <  2:3640108/100‑1 (MQ=255)

                                                                                        tCACTGGGTCCCAGATGCTGGATTGCATGATCAAAACCGCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGACg      <  2:183068/99‑1 (MQ=255)

                                                                                        tCACTGCGTCCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGACGc     >  1:3894855/1‑100 (MQ=255)

                                                                                        tCACTGCGTCCCAGATGCTGGATTCCATGAACAAAAACCCACGCCCGACTCGCGCAGAAGCTGGTGACGTTCCAAACGCCATCCTCGACGGTACTGACg      >  2:2763492/1‑99 (MQ=255)

                                                                                         cACTGCGTCCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGACGCa    <  1:1989057/100‑1 (MQ=255)

                                                                                          aCTGCGTCCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGACGCAg   >  1:1774922/1‑100 (MQ=255)

                                                                                          aCTGCGTCCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGACGCAg   >  2:3036507/1‑100 (MQ=255)

                                                                                           cTGCGTCCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGACGCAGt  >  1:1837445/1‑100 (MQ=255)

GACCTGGGTGTAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGACGCAGT  >  NC_000913/1756433‑1756623

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`