BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	1,856,334	C→A	15.4%	E199* (GAG→TAG) ‡	ydjH ←	putative kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,856,334	0	C	A	15.4%	377.8 / 46.3	136	E199* (GAG→TAG)	ydjH	putative kinase
Reads supporting (aligned to +/- strand): ref base C (68/46); new base A (9/12); total (77/59)
Fisher's exact test for biased strand distribution p-value = 2.30e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 140 total aligned reads displayed.

CGTCTTTACCCGTTTTAATCACCACCGTTTTTACGCCGCACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGTTTCATTCAACCGCGGTTTGATCATATCGGCACAGATAAT  >  NC_000913/1856235‑1856433

cGTCTTTACCCGTTTTAATCACCACCGTTTTTACGCCGCACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTc                                                                                                     >  2:3198630/1‑100 (MQ=255)

 gTCTTTACCCGTTTTAATCACCACCGTTTTTACGCCGCACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCg                                                                                                    >  1:2046455/1‑100 (MQ=255)

  tCTTTACCCGTTTTAATCACCACCGTTTTTACGCCGCACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCgg                                                                                                   <  2:2792672/100‑1 (MQ=255)

  tCTTTACCCGTTTTAATCACCACCGTTTTTACGCCGCACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCgg                                                                                                   <  1:1414857/100‑1 (MQ=255)

   cTTTACCCGTTTTAATCACCACCGTTTTTACGCCGCACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGc                                                                                                  <  1:1211253/100‑1 (MQ=255)

      tACCCGTTTTAATCACCACCGTTTTTACGCCGCACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCaaa                                                                                               >  2:3831545/1‑100 (MQ=255)

        cccGTTTTAATCACCACCGTTTTTACGCCGCACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAAt                                                                                             <  2:2243769/100‑1 (MQ=255)

        cccGTTTTAATCACCACCGTTTTTACGCCGCACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAAt                                                                                             >  2:1012256/1‑100 (MQ=255)

        cccGTTTTAATCACCACCGTTTTTACGCCGCACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCATTCCCGGTGGGTAATTTTGCCTCGGCaaaa                                                                                              >  2:2827017/1‑99 (MQ=255)

          cGTTTTAATCACCACCGTTTTTACGCCGCACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGTAAAATTa                                                                                           <  1:2193538/100‑1 (MQ=255)

          cGTTTTAATCACCACCGTTTTTACGCCGCACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTa                                                                                           <  1:1544836/100‑1 (MQ=255)

          cGTTTTAATCACCACCGTTTTTACGCCGCACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCGAGGCAAAAtt                                                                                            <  2:1945218/99‑1 (MQ=255)

           gTTTTAATCACCACCGTTTTTACGCCGCACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAg                                                                                          >  1:1655321/1‑100 (MQ=255)

           gTTTTAATCACCACCGTTTTTACGCCGCACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCGAGGCAAAATTac                                                                                          <  1:1844478/100‑2 (MQ=255)

           gTTTTAATCACCACCGTTTTTACGCCGCACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCGAGGCAAAATTac                                                                                          <  2:1956931/100‑2 (MQ=255)

           gTTTTAATCACCACCGTTTTTACGCCGCACGCAAGAAAGCAGTCAGCAATGTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCGAGGCAAAATTac                                                                                          <  2:1426584/100‑2 (MQ=255)

             tttAATCACCACCGTTTTTACGCCGCACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGa                                                                                        <  1:1849911/100‑1 (MQ=255)

             tttAATCACCACCGTTTTTACGCCGCACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGa                                                                                        <  1:1260818/100‑1 (MQ=255)

               tAATCACCACCGTTTTTACGCCGCACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGaaa                                                                                      >  1:400401/1‑100 (MQ=255)

               tAATCACCACCGTTTTTACGCCGCACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCGAGGCAAAATTactcac                                                                                      <  2:1186665/100‑6 (MQ=39)

               tAATCACCACCGTTTTTACGCCGCACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCGAGGCAAAATTactcac                                                                                      <  1:2856677/100‑6 (MQ=39)

                aaTCACCACCGTTTTTACGCCGCACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAAc                                                                                     <  1:3014698/100‑1 (MQ=255)

                aaTCACCACCGTTTTTACGCCGCACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAAc                                                                                     >  1:3285102/1‑100 (MQ=255)

                 aTCACCACCGTTTTTACGCCGCACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACa                                                                                    <  1:1031867/100‑1 (MQ=255)

                  ncaccacCGTTTTTACGCCGGACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAg                                                                                   >  2:2557519/2‑100 (MQ=255)

                   caccacCGTTTTTACGCCGCACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGa                                                                                  >  2:2671651/1‑100 (MQ=255)

                   caccacCGTTTTTACGCCGCACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGa                                                                                  >  2:43614/1‑100 (MQ=255)

                   caccacCGTTTTTACGCCGCACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGa                                                                                  <  2:2885600/100‑1 (MQ=255)

                   caccacCGATTTTACGCCGCACGCAAGAAAGCAGTCAACAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAACATTAGGAAACAGa                                                                                  >  2:2248821/1‑100 (MQ=255)

                    accaccGTTTTTACGCCGCACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGAt                                                                                 >  1:4186038/1‑100 (MQ=255)

                    accaccGATTTCACGCCGCACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGAt                                                                                 >  2:2206178/1‑100 (MQ=255)

                     ccaccGTTTTTACGCCGCACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATa                                                                                >  1:987206/1‑100 (MQ=255)

                     ccaccGTTTTTACGCCGCACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATa                                                                                >  1:671927/1‑100 (MQ=255)

                     ccaccGTTTTTACGCCGCACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATa                                                                                >  2:1123681/1‑100 (MQ=255)

                      caccGTTTTTACGCCGCACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATaa                                                                               >  2:2268640/1‑100 (MQ=255)

                      caccGTTTTTACGCCGCACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATaa                                                                               >  1:2321126/1‑100 (MQ=255)

                      caccGTTTTTACGCCGCACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATaa                                                                               >  1:3654441/1‑100 (MQ=255)

                         cGTTTTTACGCTGCATGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATc                                                                             >  2:2288603/1‑99 (MQ=255)

                         cGTTTTTACGCCGCACGCAAGAAAGCAGTCAGCAATTTCCTCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCg                                                                            >  1:3543182/1‑100 (MQ=255)

                         cGTTTTTACGCCGCACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCg                                                                            >  1:2355697/1‑100 (MQ=255)

                          gTTTTTACGCCGCACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGa                                                                           <  1:4132027/100‑1 (MQ=255)

                                aCGCCGCACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAAc                                                                     <  1:2206183/100‑1 (MQ=255)

                                 cgccgcACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACt                                                                    >  1:3528298/1‑100 (MQ=255)

                                  gccgcACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTc                                                                   >  2:58771/1‑100 (MQ=255)

                                  gccgcACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTc                                                                   >  1:114257/1‑100 (MQ=255)

                                   ccgcACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCa                                                                  >  2:293442/1‑100 (MQ=255)

                                     gcacgcaAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAAt                                                                <  1:1915875/100‑1 (MQ=255)

                                     gcacgcaAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAAt                                                                >  1:1485853/1‑100 (MQ=255)

                                      catgcaAGAAAGCAGTCAGCAATTTCATCCAGTGTCACTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATg                                                               >  1:2964697/4‑100 (MQ=255)

                                       atgctttaaaGCTTTCAGCTATTTAATCCAGTGTCCCTTTCCCGGTGGGTAATTTTGCCGCGGAAAGTTTAGGTAACAGATAATCGACATAACTCTAtt                                                               <  2:3921240/92‑2 (MQ=255)

                                        cgcaAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCt                                                             >  1:2725425/1‑100 (MQ=255)

                                          caAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTc                                                           >  1:1412396/1‑100 (MQ=255)

                                            aGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGc                                                         <  2:3708119/100‑1 (MQ=255)

                                             gAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCTCa                                                        <  2:2151194/100‑1 (MQ=255)

                                             gAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCa                                                        <  1:746290/100‑1 (MQ=255)

                                             gAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCa                                                        <  2:1418169/100‑1 (MQ=255)

                                               aaGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCGAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCaaa                                                      >  2:2688407/1‑100 (MQ=255)

                                                 gCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAata                                                    >  1:4146424/1‑100 (MQ=255)

                                                  cAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAatat                                                   <  1:3731406/100‑1 (MQ=255)

                                                    gTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATatca                                                 >  1:39650/1‑100 (MQ=255)

                                                    gTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCACAAATatca                                                 >  2:2903223/1‑100 (MQ=255)

                                                      cAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATatcatc                                               >  1:3100947/1‑100 (MQ=255)

                                                      cAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTACCTCGGCAAAATTAGGAAAGAGATACTCGACATAACTCAATGCTTCGCAAATatcat                                                >  2:2289893/1‑99 (MQ=255)

                                                       aGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATcc                                              >  2:1107610/1‑100 (MQ=255)

                                                        gCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCa                                             >  2:964650/1‑100 (MQ=255)

                                                           aTTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCAGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCg                                          >  1:3653147/1‑100 (MQ=255)

                                                              tCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGttt                                       >  1:2167519/1‑100 (MQ=255)

                                                               cATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGTttc                                      <  2:39650/100‑1 (MQ=255)

                                                                aTCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGTttca                                     >  1:626344/1‑100 (MQ=255)

                                                                 tCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGTttcat                                    <  1:1640521/100‑1 (MQ=255)

                                                                 tCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGTttcat                                    <  1:1720417/100‑1 (MQ=255)

                                                                   cAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGTttcattc                                  >  2:1814/1‑100 (MQ=255)

                                                                      tgactctTTCCCTGTCAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGTTTCATTCAAc                               <  2:2661782/97‑1 (MQ=255)

                                                                         ctctTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGTTTCATTCAACcgc                            >  2:2702775/1‑100 (MQ=255)

                                                                          tctTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGTTTCATTCAACcgcg                           >  1:2495500/1‑100 (MQ=255)

                                                                          tctTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGTTTCATTCAACcgcg                           >  2:909319/1‑100 (MQ=255)

                                                                            tttCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGTTTCATTCAACCGCGGt                         <  1:2900257/100‑1 (MQ=255)

                                                                             ttCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGTTTCATTCAACCGCGGtt                        <  2:3100934/100‑1 (MQ=255)

                                                                              tCCCGGTGAGTAATTTTGCCTCGTCAAAATTAGGAAACAGATAATCGTCATAACTCAATGCTTCGCAAATATCATCCAGCGTTTCATTCAACCGCGGttt                       <  2:697238/100‑1 (MQ=255)

                                                                                ccGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGTTTCATTCAACCGCGGTTTGa                     >  2:1696198/1‑100 (MQ=255)

                                                                                  ggTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGTTTCATTCAACCGCGGTTTGATc                   <  2:3385269/100‑1 (MQ=255)

                                                                                  ggTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGTTTCATTCAACCGCGGTTTGATc                   >  1:2747115/1‑100 (MQ=255)

                                                                                  ggTGAGTAATCTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATTTCATCCAGCGTTTCATTCAACCGCGGTTTGATc                   <  2:2746832/100‑1 (MQ=255)

                                                                                     tcctAATTTTGCCGAGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGTTTCATTCAACCGCGGTTTGATCata                >  1:2525725/4‑100 (MQ=255)

                                                                                     gAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGTTTCATTCAACCGCGGTTTGATCata                <  2:2811361/100‑1 (MQ=255)

                                                                                     gAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGTTTCATTCAACCGCGGTTTGATCata                >  1:2691671/1‑100 (MQ=255)

                                                                                      cctAATTTTGCCGAGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGTTTCATTCAACCGCGGTTTGATCatat               >  1:1587223/3‑100 (MQ=255)

                                                                                      cctAATTTTGCCGAGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGTTTCATTCAACCGCGGTTTGATCata                >  1:671318/3‑99 (MQ=255)

                                                                                      cctAATTTTGCCGAGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGTTTCATTCAACCGCGGTTTGATCagat               >  2:1891544/3‑97 (MQ=255)

                                                                                       ctAATTTTGCCGAGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGTTTCATTCAACCGCGGTTTGATCATATc              >  1:1067188/2‑100 (MQ=255)

                                                                                       ctAATTTTGCCGAGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATACAGCGTTTCATTCAACCGCGGTTTAATCATATc              >  2:620392/2‑100 (MQ=255)

                                                                                         aaTTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGTTTCATTCAACCGCGGTTTGATCATATCgg            <  1:2376472/100‑1 (MQ=255)

                                                                                         aaTTTTGCCGAGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGTTTCATTCAACCGCGGTTTGATCATATCgg            >  1:2688380/1‑100 (MQ=255)

                                                                                              tGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGTTTCATTCAACCGCGGTTTGATCATATCGGCACAg       >  1:3407739/1‑100 (MQ=255)

                                                                                                ccTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGTTTCATTCAACCGCGGTTTGATCATATCGGCACAGAt     >  2:2141258/1‑100 (MQ=255)

                                                                                                ccTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGTTTCATTCAACCGCGGTTTGATCATATCGGCACAGAt     <  1:4136194/100‑1 (MQ=255)

                                                                                                ccTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGTTTCATTCAACCGCGGTTTGATCATATCGGCACAGAt     >  1:3485661/1‑100 (MQ=255)

                                                                                                ccTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGTTTCATTCAACCGCGGTTTGATCATATCGGCACAGAt     >  1:320593/1‑100 (MQ=255)

                                                                                                  tCGGCAAAATTAGAAAACAGATAATCGACATAACTCAAGGCTTCGCAAATATCATCCAGCGTTTCATTCAACCGCGGTTTGATCATATCGGCACAGATaa   >  2:2590819/1‑100 (MQ=255)

                                                                                                   cGGTAAAATTAGTAAACAGATAATCGACATGACTCAATGCGTCGCAAATATCTTCTAGCGTTGCATTCATCCGCGGTTTGATCATATCGGCTCAGa      <  2:2879637/96‑1 (MQ=255)

CGTCTTTACCCGTTTTAATCACCACCGTTTTTACGCCGCACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGTTTCATTCAACCGCGGTTTGATCATATCGGCACAGATAAT  >  NC_000913/1856235‑1856433

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`