Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,865,682 G→C 24.2% intergenic (‑46/+44) yeaE ← / ← mipA aldo‑keto reductase, methylglyoxal to acetol, NADPH‑dependent/scaffolding protein for murein synthesizing machinery

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,865,6820GC24.2% 291.2 / 102.5 146intergenic (‑46/+44)yeaE/mipAaldo‑keto reductase, methylglyoxal to acetol, NADPH‑dependent/scaffolding protein for murein synthesizing machinery
Reads supporting (aligned to +/- strand):  ref base G (53/57);  new base C (17/18);  total (71/75)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.77e-01

Only 100 of 148 total aligned reads displayed.

CCCTACGGCTGGCAGTGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGAAATCAGGCCAGTCCAGGATTTATCCACCA  >  NC_000913/1865583‑1865781
                                                                                                   |                                                                                                   
cccTACGGCTGGCAGTGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTg                                                                                                     <  2:589528/100‑1 (MQ=255)
 ccTACGGCTGGCAGTGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTTCACCACCGTAATGCAAAACAGGGCGTGa                                                                                                    >  2:1044464/1‑100 (MQ=255)
 ccTACGGCTGGCAGTGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGa                                                                                                    >  1:2446983/1‑100 (MQ=255)
  cTACGGCTGGCAGTGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGAc                                                                                                   <  1:2133053/100‑1 (MQ=255)
  cTACGGCTGGCAGTGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGAc                                                                                                   <  2:3136348/100‑1 (MQ=255)
     cGGCTGGCAGTGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGcc                                                                                                <  1:3527095/100‑1 (MQ=255)
       gCTGGCAGTGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCt                                                                                              >  1:869490/1‑100 (MQ=255)
        cTGGCAGTGAGACATCGCCACTAAAGTGAAAAATTTTTTGTTGAATTGTTTCCTCCCAGGTATTGCACCACCATAATGCCAAACAGGGCGTGACGccc                                                                                               >  2:1990754/1‑98 (MQ=255)
         tGGCAGTGAGACATCGCCACTAAATTGAATCATTTTTCGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGt                                                                                            >  2:2233993/1‑100 (MQ=255)
           gCAGTGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGAAATGCAAAACAGGGCGTCACGCCCTGttt                                                                                          <  2:1710407/100‑1 (MQ=255)
            cAGTGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGtttt                                                                                         >  2:1901038/1‑100 (MQ=255)
            cAGTGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTCACGCCCTGtttt                                                                                         <  1:2709676/100‑1 (MQ=255)
             aGTGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTCACGCCCTGttttg                                                                                        <  2:1273991/100‑2 (MQ=255)
             aGTGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTCACGCCCTGttttg                                                                                        <  2:1626081/100‑2 (MQ=255)
             aGTGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTCACGCCCTGttttg                                                                                        <  2:3119372/100‑2 (MQ=255)
             aGTGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTCACGCCCTGttttg                                                                                        <  1:3510207/100‑2 (MQ=255)
              gTGAGCCATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTGTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTCACGCCCTGttttgc                                                                                       <  1:859384/100‑3 (MQ=39)
               tGAGACATCGCCACTGAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTCACGCCCTGttttgca                                                                                      <  1:1822463/100‑4 (MQ=255)
               tGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTAt                                                                                      >  1:3876037/1‑100 (MQ=255)
               tGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTCACGCCCTGttttgca                                                                                      <  2:1474044/100‑4 (MQ=255)
               tGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCGCCCATTTATTGCACCACCGTTATGAAAAACAGGTCGTCACGCCCTGttttgc                                                                                       <  2:1255192/99‑3 (MQ=37)
                gagaCATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATg                                                                                     <  1:2384980/100‑1 (MQ=255)
                gagaCATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATg                                                                                     <  1:1576312/100‑1 (MQ=255)
                 agaCATCGCCACTAAATTGGATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCCCCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGc                                                                                    <  1:1700210/100‑1 (MQ=255)
                  gaCATCGCCACTAATTTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTACGc                                                                                    <  2:2124357/99‑1 (MQ=255)
                     aTCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACaa                                                                                >  1:733892/1‑100 (MQ=255)
                        gCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAAt                                                                             <  1:2329926/100‑1 (MQ=255)
                        gCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAAt                                                                             >  1:2208775/1‑100 (MQ=255)
                         ccACTAAATTGAATCATTTTTTTTTTTTTTTTTTACTCCCAGGTATTGCACCACAGTAATTCATAACAGGTCTTCACGCCCTGTTTTTATGca                                                                                   >  2:1360826/1‑93 (MQ=255)
                           aCTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGcc                                                                          <  2:2116126/100‑1 (MQ=255)
                           aCTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGcc                                                                          >  1:2961237/1‑100 (MQ=255)
                           aCTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTAACGCCATGTCTTTATGCACAACATGcc                                                                          >  1:323517/1‑100 (MQ=255)
                              aaaTTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTg                                                                       >  1:2412442/1‑100 (MQ=255)
                               aaTTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTATGCACAAAATGCCCTGGa                                                                     <  2:369254/100‑1 (MQ=255)
                                 ttGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTgg                                                                      <  2:3616807/98‑1 (MQ=255)
                                   gAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTTTTTTTATGCACAAAATGCCCTGGaaa                                                                   <  2:877654/99‑1 (MQ=255)
                                      tCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATg                                                               <  1:914563/100‑1 (MQ=255)
                                       cATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGc                                                              <  2:2020951/100‑1 (MQ=255)
                                        aTTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCa                                                             <  1:1078557/100‑1 (MQ=255)
                                        aTTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCa                                                             >  1:1888654/1‑100 (MQ=255)
                                        aTTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCa                                                             <  2:3080318/100‑1 (MQ=255)
                                        aTTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCa                                                             >  1:2057445/1‑100 (MQ=255)
                                           ttttGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTa                                                          <  1:1001153/100‑1 (MQ=255)
                                              tgttgCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCa                                                       <  2:2793091/100‑1 (MQ=255)
                                                  gCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAAt                                                   >  1:1323418/1‑100 (MQ=255)
                                                  gCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAAt                                                   >  2:1134887/1‑100 (MQ=255)
                                                   cATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAAtt                                                  <  2:1844261/100‑1 (MQ=255)
                                                   cATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAAtt                                                  <  1:1044464/100‑1 (MQ=255)
                                                       gTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTa                                              >  2:244051/1‑100 (MQ=255)
                                                       gTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTa                                              >  1:2674076/1‑100 (MQ=255)
                                                        tttCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAg                                             <  2:929184/100‑1 (MQ=255)
                                                         ttCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAgg                                            <  1:102079/100‑1 (MQ=255)
                                                         ttCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAgg                                            <  2:3215447/100‑1 (MQ=255)
                                                          tcctccCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGt                                           <  2:2446978/100‑1 (MQ=255)
                                                           cctccCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTg                                          >  1:2595533/1‑100 (MQ=255)
                                                            ctccCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGa                                         >  1:1269747/1‑100 (MQ=255)
                                                            ctccCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGa                                         >  1:3469940/1‑100 (MQ=255)
                                                                cAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATccc                                     <  1:1377061/100‑1 (MQ=255)
                                                                cAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATccc                                     <  1:3243116/100‑1 (MQ=255)
                                                                  ggTATTGCACCACCGTAATGCAAAACAGGGCGTGTCGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCgg                                   <  2:2208770/100‑1 (MQ=255)
                                                                  ggTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCgg                                   <  1:1397335/100‑1 (MQ=255)
                                                                     aTTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACTAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAg                                <  1:886494/100‑1 (MQ=255)
                                                                     aTTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAg                                <  2:1323417/100‑1 (MQ=255)
                                                                       tGCACCCCCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAGAGATGCATTATCAGAATCTGTAGGTGAGCCCGGTAGaa                              <  2:2564569/100‑1 (MQ=255)
                                                                        gCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGaaa                             <  1:3883012/100‑1 (MQ=255)
                                                                        gCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGaaa                             >  2:425692/1‑100 (MQ=255)
                                                                          accaccGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGAAATc                           <  1:2423956/100‑1 (MQ=255)
                                                                          accaccGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGAAATc                           >  2:1714461/1‑100 (MQ=255)
                                                                           ccaccGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGAAATCa                          >  1:2199781/1‑100 (MQ=255)
                                                                            caccGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAACTGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGAAATCAg                         <  1:1618204/100‑1 (MQ=255)
                                                                            caccGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGAAATCAg                         <  1:2777335/100‑1 (MQ=255)
                                                                             accGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGAAATCAgg                        <  1:1250777/100‑1 (MQ=255)
                                                                             accGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGAAATCAgg                        >  1:1568371/1‑100 (MQ=255)
                                                                              ccGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGAAATCAgg                        <  2:2781779/99‑1 (MQ=255)
                                                                              ccGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGAAATCAGGc                       <  2:2378153/100‑1 (MQ=255)
                                                                                 tAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGAAATCAGGCCAg                    >  2:3049564/1‑100 (MQ=255)
                                                                                  gcataaaaaCAGGGCGTCACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGAAATCAGGCCAGt                   >  1:2025591/6‑100 (MQ=255)
                                                                                  gcataaaaaCAGGGCGTCACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGAAATCAGGCCAGt                   >  1:922289/6‑100 (MQ=255)
                                                                                  gcataaaaaCAGGGCGTCACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGACGCATTATCAGAATTTGTAGGTGATCCCTGAAGAAATCAGGCCAGt                   >  2:1605697/6‑100 (MQ=38)
                                                                                   cataaaaaCAGGGCGTCACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGAAATCAGGCCAg                    >  1:3288552/5‑98 (MQ=255)
                                                                                   cataaaaaCAGGGCGTCACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGAAATCAGGCCAGTc                  >  1:1830012/5‑100 (MQ=255)
                                                                                   cataaaaaCAGGGCGTCACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGAAATCAGGCCAGTc                  >  1:2314774/5‑100 (MQ=255)
                                                                                    tGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGAAATCAGGCCAGTcc                 >  2:349165/1‑100 (MQ=255)
                                                                                    tGCAAAACAGGGCGGGACGAGCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGAAATCATGCCAGTcc                 <  2:56625/100‑1 (MQ=255)
                                                                                    ataaaaaCAGGGCGTCACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGAAATCAGGCCAGTcc                 >  1:3326696/4‑100 (MQ=255)
                                                                                     taaaaaCAGGGCGTCACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGAAATCAGGCCAGTCCa                >  1:2995198/3‑100 (MQ=255)
                                                                                     gCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGAAATCAGGCCAGTCCa                >  2:158320/1‑100 (MQ=255)
                                                                                     gCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGAAATCAGGCCAGTCCa                >  1:2906062/1‑100 (MQ=255)
                                                                                      cAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGAAATCAGGCCAGTCCAg               <  1:166106/100‑1 (MQ=255)
                                                                                       aaaaCAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGAAATCAGGCCAGTCCAgg              <  2:3764164/100‑1 (MQ=255)
                                                                                       aaaaCAGGGCGTCACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTCTCAGAATTTGTAGGTGATCCCGGTAGAAATCAGGCCAGTCCAgg              >  2:3285431/1‑100 (MQ=255)
                                                                                       aaaaCAGGGCGTCACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGAAATCAGGCCAGTCCAgg              >  2:683769/1‑100 (MQ=255)
                                                                                        aaaCAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGAAATCAGGCCAGTCCAGGa             >  2:703669/1‑100 (MQ=255)
                                                                                         aaCAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGAAATCAGGCCAGTCCAGGAt            <  1:1464254/100‑1 (MQ=255)
                                                                                            aGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGAAATCAGGCCAGTCCAGGATTTa         <  1:702778/100‑1 (MQ=255)
                                                                                              ggCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGAAATCAGGCCAGTCCAGGATTTATc       >  1:4077236/1‑100 (MQ=255)
                                                                                                cGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGAAATCAGGCCAGTCCAGGATTTATcca     >  1:4031113/1‑100 (MQ=255)
                                                                                                cGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGAAATCAGGCCAGTCCAGGATTTATcca     <  1:2763163/100‑1 (MQ=255)
                                                                                                 gTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGAAATCAGGCCAGTCCAGGATTTATccac    <  2:3257082/100‑1 (MQ=255)
                                                                                                   gACGCCCTGTTTTTATGCACAAAATGCCCTGGCCAGATGCATTATCAGAACTTGTAGGTGATCCCGGTAGACCTCAGGCCAGTCCAGGATTTATccacca  >  2:262159/1‑100 (MQ=255)
                                                                                                   |                                                                                                   
CCCTACGGCTGGCAGTGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGAAATCAGGCCAGTCCAGGATTTATCCACCA  >  NC_000913/1865583‑1865781

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: