Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,296,0600CT30.6% 276.2 / 136.5 170intergenic (+266/+376)gltP/yjcOglutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein
Reads supporting (aligned to +/- strand):  ref base C (62/56);  new base T (31/21);  total (93/77)
Fisher's exact test for biased strand distribution p-value = 4.09e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.29e-01

Only 100 of 170 total aligned reads displayed.

TTAACGCGTCTTATCAGGCCTACGCCAGACAGCGC‑AATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCC  >  NC_000913/4295962‑4296158
                                                                                                   |                                                                                                  
ttAACGCGTCTTATCAGGCCTACGCCAGACAGCGC‑AATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACg                                                                                                   <  1:904707/100‑1 (MQ=33)
 tAACGCGTCTTATCAGGCCTACGCCAGACAGCGC‑AATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGc                                                                                                  <  1:698377/100‑1 (MQ=18)
 tAACGCGTCTTATCAGGCCTACGCCAGACAGCGC‑AATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGc                                                                                                  <  2:181579/100‑1 (MQ=33)
 tAACGCGTCTTATCAGGCCTACGCCAGACAGCGC‑AATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGc                                                                                                  >  1:921384/1‑100 (MQ=33)
 tAACGCGTCTTATCAGGCCTACGCCAGACAGCGC‑AATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGc                                                                                                  <  2:179743/100‑1 (MQ=33)
  aaCGCGTCTTATCAGGCCTACGCCAGACAGCGC‑AATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGcc                                                                                                 >  1:1177261/1‑100 (MQ=32)
  aaCGCGTCTTATCAGGCCTACGCCAGACAGCGC‑AATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGcc                                                                                                 <  1:1774061/100‑1 (MQ=33)
    cgcgTCTTATCAGGCCTACGCCAGACAGCGC‑AATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTg                                                                                               <  2:771911/100‑1 (MQ=18)
    cgcgTCTTATCAGGCCTACGCCAGACAGCGC‑AATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTg                                                                                               <  2:589989/100‑1 (MQ=32)
      cgTCTTATCAGGCCTACGCCAGACAGCGC‑AATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGAt                                                                                             >  2:244538/1‑100 (MQ=18)
       gTCTTATCAGGCCTACGCCAGACAGCGC‑AATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATg                                                                                            <  2:1393645/100‑1 (MQ=33)
        tCTTATCAGGCCTACGCCAGACAGCGC‑AATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGc                                                                                           >  1:1592094/1‑100 (MQ=17)
        tCTTATCAGGCCTACGCCAGACAGCGC‑AATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGc                                                                                           <  1:2071608/100‑1 (MQ=18)
        tCTTATCAGGCCTACGCCAGACAGCGC‑AATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGc                                                                                           >  1:689921/1‑100 (MQ=32)
        tCTTATCAGGCCTACGCCAGACAGCGC‑AATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGc                                                                                           <  2:2743052/100‑1 (MQ=33)
         cTTATCAGGCCTACGCCAGACAGCGC‑AATAGCCTGATCTGGAGTGCTTTTGGAAGTGAGAGAGGGCGATTGCGCGTAATCCTAGATGAACGCCGGAt                                                                                             >  2:1510274/1‑97 (MQ=2)
           tATCAGGCCTACGCCAGACAGCGC‑AATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGAc                                                                                        >  1:1031577/1‑100 (MQ=18)
           tATCAGGCCTACGCCAGACAGCGC‑AATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGAc                                                                                        >  2:272940/1‑100 (MQ=18)
            aTCAGGCCTACGCCAGACAGCGC‑AATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACg                                                                                       >  1:973769/1‑100 (MQ=18)
             tCAGGCCTACGCCAGACAGCGC‑AATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGc                                                                                      >  1:950693/1‑100 (MQ=33)
              cAGGCCTACGCCAGACAGCGC‑AATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCt                                                                                     >  2:1615958/1‑100 (MQ=32)
              cAGGCCTACGCCAGACAGCGC‑AATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCt                                                                                     >  2:1616543/1‑100 (MQ=33)
              cAGGCCTACGCCAGACAGCGC‑AATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCt                                                                                     >  1:1924698/1‑100 (MQ=33)
                ggCCTACGCCAGACAGCGC‑AATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGGCGCATCCGACATCAACGCCTGATGCGACGCTTg                                                                                   <  1:1380506/100‑1 (MQ=18)
                ggCCTACGCCAGACAGCGC‑AATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCtt                                                                                    >  2:975496/1‑99 (MQ=17)
                ggCCTACGCCAGACAGCGC‑AATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTg                                                                                   >  1:1356315/1‑100 (MQ=25)
                ggCCTACGCCAGACAGCGC‑AATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTg                                                                                   <  1:2645656/100‑1 (MQ=25)
                ggCCTACGCCAGACAGCGC‑AATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGTGACGCTTg                                                                                   >  1:2134163/1‑100 (MQ=25)
                ggCCTACGCCAGACAGCGC‑AATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTGACGCCGCATCCGACCTCAACGCCTGATGCGa                                                                                         >  2:1900459/1‑94 (MQ=12)
                 gattACGCCAGCCAGACC‑CATAGCCTGATTTATAGTGATGTTATAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGc                                                                                  <  2:1977119/97‑1 (MQ=11)
                 gCCTACGCCCGACAACCCAAATACCCTAATTTA‑CGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGc                                                                                           <  2:1840388/91‑1 (MQ=11)
                  ccTACGCCAGACAGCGC‑AATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTAcc                                                                                 >  2:1466720/1‑100 (MQ=14)
                   cTACGCCAGACAGCGC‑AATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCcg                                                                                >  2:1948277/1‑100 (MQ=25)
                      cGCCAGACAGCGC‑AATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGt                                                                             >  1:27971/1‑100 (MQ=25)
                      cGCCAGACAGCGC‑AATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGt                                                                             >  2:2647042/1‑100 (MQ=25)
                      cGCCAGACAGCGC‑AATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGt                                                                             >  1:899498/1‑100 (MQ=38)
                       gCCAGACAGCGC‑AATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTACCGCGTc                                                                            >  2:269374/1‑100 (MQ=17)
                        ccAGACAGCGC‑AATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCt                                                                           >  2:573265/1‑100 (MQ=25)
                        ccAGACAGCGC‑AATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCt                                                                           >  2:279305/1‑100 (MQ=25)
                        ccAGACAGCGC‑AATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCt                                                                           <  1:2423864/100‑1 (MQ=255)
                         cAGACAGCGT‑TTTAGCCTGATTTAGCGTGATTTTGTTGGTCGGTTAAGGAGTTTATGCCGCATCGGAGATCAATGCCTGATGCGACGCTTGCCGCGTCt                                                                           <  2:1277296/99‑1 (MQ=11)
                          aGACAGCGC‑AATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTa                                                                         >  2:1385619/1‑100 (MQ=17)
                          aGACAGCGC‑AATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTa                                                                         >  1:1113059/1‑100 (MQ=25)
                          aGACAGCGC‑AATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTa                                                                         >  2:947075/1‑100 (MQ=40)
                           gACAGCGC‑AATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTAt                                                                        <  2:1552938/100‑1 (MQ=25)
                            aCAGCGC‑AATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATc                                                                       <  1:1394135/100‑1 (MQ=255)
                              aGCGG‑AATCGCTTCATTTCGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCt                                                                           <  2:1296526/94‑1 (MQ=11)
                                cgc‑AATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGc                                                                   >  1:686698/1‑100 (MQ=255)
                                 gc‑AATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGcc                                                                  <  1:152424/100‑1 (MQ=25)
                                 gc‑AATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGcc                                                                  <  2:689918/100‑1 (MQ=255)
                                 gc‑AATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGcc                                                                  <  1:1616549/100‑1 (MQ=255)
                                 gc‑AATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGcc                                                                  <  1:1615964/100‑1 (MQ=40)
                                  c‑AATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCt                                                                 >  2:741214/1‑100 (MQ=25)
                                  c‑AATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCt                                                                 >  1:566588/1‑100 (MQ=255)
                                    aaTAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTa                                                                <  1:974907/100‑1 (MQ=25)
                                     aTAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTa                                                                <  2:657761/99‑1 (MQ=25)
                                     aTAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGTGACGCTTGCCGCGTCTTATCAGGCCTAc                                                               <  1:785764/100‑1 (MQ=255)
                                      tAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACg                                                              <  2:1829549/100‑1 (MQ=255)
                                        gCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTatctt                                                            >  1:1047228/1‑96 (MQ=9)
                                        gCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTatctt                                                            >  2:2151805/1‑96 (MQ=9)
                                        gCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGcc                                                            >  1:2471304/1‑100 (MQ=255)
                                        gCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGcc                                                            >  1:1320853/1‑100 (MQ=255)
                                        gCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGcc                                                            >  1:2821040/1‑100 (MQ=255)
                                        gCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGAATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTatctt                                                            >  2:1568489/1‑96 (MQ=11)
                                          cTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAg                                                          >  1:2799685/1‑100 (MQ=255)
                                            gATCTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGAc                                                        <  2:2715051/100‑1 (MQ=255)
                                               ttAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAgc                                                     >  2:2392594/1‑100 (MQ=14)
                                                  gCGTGATTTTGTTGGTCGGCTATGGCGTTTACGGCGCCTCCGCCATCATCGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGAc                                                        <  2:278128/94‑1 (MQ=14)
                                                  gCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCa                                                  >  1:902684/1‑100 (MQ=255)
                                                   cGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCaa                                                 >  1:652449/1‑100 (MQ=255)
                                                    gTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAAt                                                >  2:617499/1‑100 (MQ=40)
                                                    gTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAAt                                                <  1:2392605/100‑1 (MQ=255)
                                                        ttttGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGcc                                            <  1:2051903/100‑1 (MQ=255)
                                                        ttttGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGcc                                            <  1:1628002/100‑1 (MQ=255)
                                                        ttttGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGcc                                            >  1:1727318/1‑100 (MQ=255)
                                                          ttGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTg                                          <  2:2133069/100‑1 (MQ=40)
                                                          ttGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTg                                          <  2:844556/100‑1 (MQ=40)
                                                           tGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGa                                         >  2:2364554/1‑100 (MQ=255)
                                                             tagctctcaTAAGGTGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCt                                           <  2:2332726/88‑1 (MQ=14)
                                                              aGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGAttt                                      >  1:372543/1‑100 (MQ=255)
                                                                  cGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTACCGCGTCGGATCAGGCCTACGCCAGACAGCGCAAGAGCCTGATTTAGCg                                  >  1:1977128/1‑100 (MQ=11)
                                                                      tAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTATCGTGa                               >  2:1102158/1‑99 (MQ=255)
                                                                      tAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGAt                              >  2:462014/1‑100 (MQ=255)
                                                                        aGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGAttt                            <  1:2690354/100‑1 (MQ=255)
                                                                         ggCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGAtttt                           >  1:486807/1‑100 (MQ=255)
                                                                           cGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGt                         >  1:1299389/1‑100 (MQ=255)
                                                                              ttACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAgg                      >  1:2598569/1‑100 (MQ=255)
                                                                                aCGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTc                    >  1:147905/1‑100 (MQ=40)
                                                                                 cgccgcATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCg                   >  2:1608547/1‑100 (MQ=38)
                                                                                 cgccgcATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGACTACGCGAGACAGGGCTATAGCATGATTTAGCGTGCTTTTGTATGTCg                   >  2:1676123/1‑100 (MQ=14)
                                                                                   ncgcATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGCTTTTGTAGGTCg                   >  2:2482708/2‑98 (MQ=255)
                                                                                    cgcATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGAt                <  1:740566/100‑1 (MQ=35)
                                                                                       aTCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAg             <  1:1172708/100‑1 (MQ=38)
                                                                                             cATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGttt       >  1:776431/1‑100 (MQ=38)
                                                                                               tCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTAt     >  1:1880439/1‑100 (MQ=35)
                                                                                                cAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATg    <  1:1162514/100‑1 (MQ=33)
                                                                                                cAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATg    >  1:2757380/1‑100 (MQ=33)
                                                                                                cAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTAATg    >  2:341534/1‑100 (MQ=18)
                                                                                                 aaCGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGc   <  1:1608553/100‑1 (MQ=32)
                                                                                                 aaCGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGGGATTTTGTAGGTCGGATAAAGCGTTTAtac   >  2:2645644/1‑98 (MQ=255)
                                                                                                   |                                                                                                  
TTAACGCGTCTTATCAGGCCTACGCCAGACAGCGC‑AATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCC  >  NC_000913/4295962‑4296158

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑