Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,756,553	A→T	K286* (AAA→TAA)	pykF →	pyruvate kinase I

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,756,553	0	A	T	100.0%	311.4 / NA	88	K286* (AAA→TAA)	pykF	pyruvate kinase I
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (47/41);  total (47/41)

AGAAGAAGTTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTGAATCCGCAAAA  >  NC_000913/1756456‑1756648                                                                                                  |                                                                                                agaagaAGTTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCtaa                                                                                               <  1:2466549/100‑1 (MQ=255)  gaagaaGTTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCtaaa                                                                                              <  2:1498012/100‑1 (MQ=255)   cttccgatctCTTCGCCCAGAAGATGATGATCCAAAAATATATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCtaaaa                                                                                             <  2:1980453/91‑1 (MQ=255)    agaagTTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCTAAAAc                                                                                            <  2:1076846/100‑1 (MQ=255)    agaagTTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCTAAAAc                                                                                            <  2:1454432/100‑1 (MQ=255)     gaagTTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCTAAAAcc                                                                                           >  1:2709143/1‑100 (MQ=255)      aagTTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCTAAAAccc                                                                                          >  2:2201943/1‑100 (MQ=255)      aagTTATCTTCGCCCAGAAGATGATGATCGAAAAATCTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCTAAAAccc                                                                                          >  1:1989766/1‑100 (MQ=255)       agTTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCTAAAACCCa                                                                                         >  2:614150/1‑100 (MQ=255)        gTTATCTTCGCCCAGAAGATGATGATAGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCTTAAACCCa                                                                                         >  2:126849/1‑99 (MQ=255)          tATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCTAAAACCCACGc                                                                                      >  2:2325714/1‑100 (MQ=255)            tCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCTAAAACCCACGcc                                                                                     <  2:1013511/99‑1 (MQ=255)             cTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCtaaaaagatcggaag                                                                                   >  1:1980462/1‑90 (MQ=255)             cTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCTAAAACCCACGCCCg                                                                                   <  1:2645154/100‑1 (MQ=255)              ttCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCTAAAACCCACGCCCGa                                                                                  >  2:2753102/1‑100 (MQ=255)                cGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCTAAAACCCACGCCCGACt                                                                                <  1:231541/100‑1 (MQ=255)                 gCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCGTGATCTAAAACCCACGCCCGACTc                                                                               <  2:95481/100‑1 (MQ=255)                 gCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCTAAAACCCACGCCCGACTc                                                                               >  2:1721064/1‑100 (MQ=255)                  cccAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCTAAAACCCACGCCCGACTcg                                                                              <  2:2016920/100‑1 (MQ=255)                   ccAGAAGATGATGATCGAAAAATTTATCCGTGCACGTAAAGTCGTTATCGCTGCGACCCAGATGCTGGATTCCATGATCTAAAACCCACGCCCGACTcg                                                                              <  2:720992/99‑1 (MQ=255)                    cAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCTAAAACCCACGCCCGACTcgcg                                                                            <  2:1019582/100‑1 (MQ=255)                     agaagaTGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCTAAAACCCACGCCCGACTCgcgc                                                                           >  1:660820/1‑100 (MQ=255)                     agaagaTGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCTAAAACCCACGCCCGACTCgcgc                                                                           >  2:750907/1‑100 (MQ=255)                     agaagaTGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCTAAAACCCACGCCCGACTCgcgc                                                                           <  1:2484491/100‑1 (MQ=255)                      gaagaTGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGACGCTGGATTCCATGATCTAAAACCCACGCCCGACTCGCGCa                                                                          <  1:1033868/100‑1 (MQ=255)                       aagaTGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCTAAAACCCACGCCCGACTCGCGCAg                                                                         <  1:511428/100‑1 (MQ=255)                       aagaTGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCTAAAACCCACGCCCGACTCGCGCAg                                                                         >  2:951294/1‑100 (MQ=255)                              tgatCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCTAAAACCCACGCCCGACTCGCGCAGAAGCCgg                                                                  >  1:2611749/1‑100 (MQ=255)                              tgatCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCTAAAACCCACGCCCGACTCGCGCAGAAGCCgg                                                                  >  2:2031252/1‑100 (MQ=255)                              tgatCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCTAAAACCCACGCCCGACTCGCGCAGAAGCCgg                                                                  <  2:2284842/100‑1 (MQ=255)                              tgatCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCTAAAACCCACGCCCGACTCGCGCAGAAGCCgg                                                                  <  2:2789524/100‑1 (MQ=255)                              ngatCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCTAAAACCCACGCCCGACTCGCGCAGAAGCCgg                                                                  >  2:77519/2‑100 (MQ=255)                               gatCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCTAAAACCCACGCCCGACTCGCGCAGAAGCCgg                                                                  >  2:2200993/1‑99 (MQ=255)                               gatCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCTAAAACCCACGCCCGACTCGCGCAGAAGCCGGt                                                                 >  2:2291595/1‑100 (MQ=255)                               gatCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCTAAAACCCACGCCCGACTCGCGCAGAAGCCGGt                                                                 >  2:2323887/1‑100 (MQ=255)                               gatCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCTAAAACCCACGCCCGACTCGCGCAGAAGCCGGt                                                                 <  2:2569006/100‑1 (MQ=255)                               gatCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCTAAAACCCACGCCCGACTCGCGCAGAAGCCGGt                                                                 >  2:546275/1‑100 (MQ=255)                               gatCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCTAAAACCCACGCCCGACTCGCGCAGAAGCCGGt                                                                 >  2:711797/1‑100 (MQ=255)                               gatCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCTAAAACCCACGCCCGACTCGCGCAGAAGCCGGt                                                                 <  1:1128199/100‑1 (MQ=255)                               gatCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCTAAAACCCACGCCCGACTCGCGCAGAAGCCGGt                                                                 <  1:2201954/100‑1 (MQ=255)                                atCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCTAAAACCCACGCCCGACTCGCGCAGAAGCCGGTg                                                                >  2:1190104/1‑100 (MQ=255)                                atCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCTAAAACCCACGCCCGACTCGCGCAGAAGCCGGTg                                                                >  2:1393124/1‑100 (MQ=255)                                atCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCTAAAACCCACGCCCGACTCGCGCAGAAGCCGGTg                                                                >  2:320644/1‑100 (MQ=255)                                atCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCTAAAACCCACGCCCGACTCGCGCAGAAGCCGGTg                                                                <  1:2474877/100‑1 (MQ=255)                                atCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCTAAAACCCACGCCCGACTCGCGCAGAAGCCGGTg                                                                <  2:1987954/100‑1 (MQ=255)                                  cGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCTAAAACCCAAGCCCGACTCTCGCAGAAGCCGGTGAc                                                              >  2:1649946/1‑100 (MQ=255)                                   gAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCTAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACg                                                             >  2:1232925/1‑100 (MQ=255)                                   gAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCTAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACg                                                             >  2:2490915/1‑100 (MQ=255)                                    aaaaaTGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCTAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGt                                                            >  2:1190239/1‑100 (MQ=255)                                    aaaaaTGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCTAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGt                                                            <  2:741219/100‑1 (MQ=255)                                      aaaTGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCTAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTg                                                          <  1:1190109/100‑1 (MQ=255)                                       aaTGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCTAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGc                                                         <  2:2583925/100‑1 (MQ=255)                                       aaTGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCTAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGc                                                         <  2:220130/100‑1 (MQ=255)                                                   cACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCTAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCt                                             >  1:241478/1‑100 (MQ=255)                                                     cGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCTAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCg                                           >  1:2282747/1‑100 (MQ=255)                                                     cGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCTAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCg                                           >  1:1337731/1‑100 (MQ=255)                                                       tAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCTAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGAc                                         <  2:1148868/100‑1 (MQ=255)                                                       tAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCTAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGAc                                         <  1:750910/100‑1 (MQ=255)                                                       tAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCTAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGAc                                         <  1:951297/100‑1 (MQ=255)                                                         aaGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCTAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACgg                                       <  2:2709131/100‑1 (MQ=255)                                                            tCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCTAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTAc                                    <  2:2500093/100‑1 (MQ=255)                                                             cGTTATCACTGCGACCCAGATGCTGGATTCCATGATCTAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACt                                   >  1:1566823/1‑100 (MQ=255)                                                             cGTTATCACTGCCACCCATATTCGGGATTTCATGATCTAAAACCCACTCTCGACTCGTCCATAATCCGTTGACGTTGCAAACGCCATCCTCg                                           <  2:1473862/92‑1 (MQ=255)                                                             cGTCATCACTGCGACCCCGATGCTGGATTCCAAGATCTAAAACTCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGCCGGTa                                     <  2:2535378/98‑1 (MQ=255)                                                              gTTATCACTGCGACCCAGATGCTGGATTCCATGATCTAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTg                                  >  1:2022891/1‑100 (MQ=255)                                                                  tCACTGCGACCCAGATGCTGGATTCCATGATCTAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGACGc                              >  2:1655922/1‑100 (MQ=255)                                                                    aCTGCGACCCAGATGCTGGATTCCATGATCTAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGACGCAg                            >  1:1299101/1‑100 (MQ=255)                                                                     cTGCGACCCAGATGCTGGATTCCATGATCTAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGACGCAGt                           >  2:1885845/1‑100 (MQ=255)                                                                      tGCGACCCAGATGCTGGATTCCATGATCTAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGACGCAGTg                          <  1:2031262/100‑1 (MQ=255)                                                                         gACCCAGATGCTGGATTCCATGATCTAAAGCCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATg                       <  2:660817/100‑1 (MQ=255)                                                                         gACCCAGATGCTGGAGTCCATGATGTAAAGCACACGGCCGTCTCGCCCAGAAGACGGTGACGTTGCAAACGCCATCCTGGACGGTACTGACGCAGTGATg                       <  2:2618008/100‑1 (MQ=255)                                                                          aCCCAGATGCTGGATTCCATGATCTAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGc                      <  1:2366673/100‑1 (MQ=255)                                                                           cccAGATGCTGGATTCCATGATCTAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCt                     >  2:1413918/1‑100 (MQ=255)                                                                           cccAGATGCTGGATTCCATGATCTAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCt                     >  1:1841996/1‑100 (MQ=255)                                                                             cAGATGCTGGATTCCATGATCTAAAACCCACGCCAGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGATGGTACTGACGCAGTGATGc                      >  2:941481/1‑97 (MQ=39)                                                                               gctGCTGGATTCCATGATCTAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCt                 <  2:1713476/98‑1 (MQ=255)                                                                                 tGCTGGATTCCATGATCTAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTgg               >  1:1920754/1‑100 (MQ=255)                                                                                 tGCTGGATTCCATGATCTAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTgg               >  1:2459858/1‑100 (MQ=255)                                                                                  gCTGGATTCCATGATCTAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGt              >  1:1977510/1‑100 (MQ=255)                                                                                   ctgcactCCATGATCTAAAACCCACGCCCGACTCGCGCAGAAGACGGTGACGTTGCAAACTTCATCTTCGTCGGTACTGACGCAGTGATGTTTTCTtgtg             <  2:935978/94‑1 (MQ=255)                                                                                   cTGGATTCCATGATCTAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTg             >  1:2670816/1‑100 (MQ=255)                                                                                   cTGGATTCCATGATCTAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTg             <  1:46353/100‑1 (MQ=255)                                                                                   cTGGATTCCATGATCTAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTg             <  1:1393130/100‑1 (MQ=255)                                                                                    tGGATTCCATGATCTAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTGa            >  2:1890356/1‑100 (MQ=255)                                                                                     ggATTCCATGATCTAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTGaa           >  2:2326106/1‑100 (MQ=255)                                                                                        ttCCATGATCTAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTGAATcn        <  1:1620688/100‑2 (MQ=255)                                                                                        ttCCATGATCTAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTGAATcc        >  2:1520215/1‑100 (MQ=255)                                                                                          ccATGATCTAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTGAATCCGc      >  2:1948834/1‑100 (MQ=255)                                                                                           cATGATCTAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTGAATCCGCa     <  2:2676632/100‑1 (MQ=255)                                                                                             tGATCTAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTGAATCCGCaaa   >  1:490060/1‑100 (MQ=255)                                                                                              gATCTAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTGAATCCGCaaaa  >  2:471239/1‑100 (MQ=255)                                                                                              gATCTAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTGAATCCGCaaaa  <  1:2490927/100‑1 (MQ=255)                                                                                                  |                                                                                                AGAAGAAGTTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTGAATCCGCAAAA  >  NC_000913/1756456‑1756648

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑