BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	2,018,260	(A)_8→7	100%	coding (315/444 nt)	fliJ →	flagellar protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,018,253	0	A	.	95.0%	430.8 / 14.1	101	coding (308/444 nt)	fliJ	flagellar protein
Reads supporting (aligned to +/- strand): ref base A (0/5); new base . (39/57); total (39/62)
Fisher's exact test for biased strand distribution p-value = 1.53e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 114 total aligned reads displayed.

AGCAGTTTATCCAGACGCTGGAAAAAGCCATTACTCAGCATCGCCAGCAACTTAATCAGTGGACGCAGAAAGTTGACATTGCCCTGAACAGTTGGCGAGAAAAAAAACAACGTTTGCAGGCCTGGCAGACACTGCAGGAACGGCAATCCACGGCGGCACTGCTTGCAGAAAACCGCCTCGATCAGAAAAAGATGG  >  NC_000913/2018154‑2018348

agcagTTTATCCAGACGCTGGAAAAAGCCATTACTCAGCATCGCCAGCAACTTAATCAGTGGACGCAGAAAGTTGACATTGCCCTGAACAGTTGGCgaga                                                                                                 >  2:2726671/1‑100 (MQ=255)

accagTTTATCCAGACGCTGGAAAAAGCCATTACTCAGCATCGCCAGCAACTTAATCAGTGGACGCAGAAAGTTGACATTGCCCTGAACAGTTGGCgaga                                                                                                 <  2:3154474/98‑1 (MQ=255)

 ncagTTTATCCAGACGCTGGAAAAAGCCATTACTCAGCATCGCCAGCAACTTAATCAGTGGACGCAGAAAGTTGACATTGCCCTGAACAGTTGGCGAGaa                                                                                                >  2:1176155/2‑100 (MQ=255)

  cagTTTATCCAGACGCTGGAAAAAGCCATTACTCAGCATCGCCAGCAACTTAATCAGTGGACGCAGAAAGTTGACATTGCCCTGAACAGTTGGCGAGaaa                                                                                               >  2:2185107/1‑100 (MQ=255)

      ttATCCAGACGCTGGAAAAAGCCATTACTCAGCATCGCCAGCAACTTAATCAGTGGACGCAGAAAGTTGACATTGCCCTGAACAGTTGGCGAGaaaaaaa                                                                                           >  1:2108030/1‑100 (MQ=255)

       tATCCAGACGCTGGAAAAAGCCATTACTCAGCATCGCCAGCAACTTAATCAGTGGACGCAGAAAGTTGACATTGCCCTGAACAGTTGGCGAGaaaaaaac                                                                                          <  1:848809/100‑2 (MQ=255)

         tCCAGACGCTGGAAAAAGCCATTACTCAGCATCGCCAGCAACTTAATCAGTGGACGCAGAAAGTTGACATTGCCCTGAACAGTTGGCGAGaaaaaaacaa                                                                                        >  1:591213/1‑97 (MQ=255)

          ccAGACGCTGGAAAAAGCCATTACTCAGCATCGCCAGCAACTTAATCAGTGGACGCAGAAAGTTGACATTGCCCTGAACAGTTGGCGAGaaaaaaacaac                                                                                       <  1:1552163/100‑5 (MQ=255)

          ccAGACGCTGGAAAAAGCCATTACTCAGCATCGCCAGCAACTTAATCAGTGGACGCAGAAAGTTGACATTGCCCTGAACAGTTGGCGAGaaaaaaacaac                                                                                       <  1:1682446/100‑5 (MQ=255)

           cAGACGCTGGAAAAAGCCATTACTCAGCATCGCCAGCAACTTAATCAGTGGACGCAGAAAGTTGACATTGCCCTGAACAGTTGGCGAG‑AAAAAAACAACg                                                                                     >  2:2567669/1‑100 (MQ=255)

               cGCTGGAAAAAGCCATTACTCAGCATCGCCAGCAACTTAATCAGTGGACGCAGAAAGTTGACATTGCCCTGAACAGTTGGCGAG‑AAAAAAACAACGTTTg                                                                                 >  1:668332/1‑100 (MQ=255)

                 cTGGAAAAAGCCATTACTCAGCACCGCCAGCAACTTAATCAGTGGACGCAGAAAGTTGACATTGCCCTGAACAAAAAAAAA‑AAAAAAAACAACGTTTGCa                                                                               <  2:2456159/100‑1 (MQ=255)

                  tGGAAAAAGCCATTACTCAGCATCGCCAGCAACTTAATCAGTGGACGCAGAAAGTTGACATTGCCCTGAACAGTTGGCGAG‑AAAAAAACAACGTTTGCAg                                                                              >  1:2081846/1‑100 (MQ=255)

                  tGGAAAAAGCCATTACTCAGCATCGCCAGCAACTTAATCAGTGGACGCAGAAAGTTGACATTGCCCTGAACAGTTGGCGAG‑AAAAAAACAACGTTTGCAg                                                                              >  2:2251062/1‑100 (MQ=255)

                  tGGAAAAAGCCATTACTCAGCATCGCCAGCAACTTAATCAGTGGACGCAGAAAGTTGACATTGCCCTGAACAGTTGGCGAG‑AAAAAAACAACGTTTGCAg                                                                              >  2:2105793/1‑100 (MQ=255)

                   ggAAAAAGCCATTACTCAGCATCGCCAGCAACTTAATCAGTGGACGCAGAAAGTTGACATTGCCCTGAACAGTTGGCGAG‑AAAAAAACAACGTTTGCAgg                                                                             >  2:864841/1‑100 (MQ=255)

                    gAAAAAGCCATTACTCAGCATCGCCAGCAACTTAATCAGTGGACGCAGAAAGTTGACATTGCCCTGAACAGTTGGCGAG‑AAAAAAACAACGTTTGCAGGc                                                                            >  1:1537228/1‑100 (MQ=255)

                      aaaaGCCATTACTCAGCATCGCCAGCAACTTAATCAGTGGACGCAGAAAGTTGACATTGCCCTGAACAGTTGGCGAG‑AAAAAAACAACGTTTGCAGGCCt                                                                          <  1:1246140/100‑1 (MQ=255)

                       aaaGCCATTACTCAGCATCGCCAGCAACTTAATCAGTGGACGCAGAAAGTTGACATTGCCCTAAACAGTTGGAGA‑AAAAAAAACAACGTTTGCAGGCCTg                                                                         <  1:1206510/100‑1 (MQ=255)

                        aaGCCATTACTCAGCATCGCCAGCAACTTAATCAGTGGACGCAGAAAGTTGACATTGCCCTGAACAGTTGGCGAG‑AAAAAAACAACGTTTGCAGGCCTgg                                                                        <  2:1802376/100‑1 (MQ=255)

                         aGCCATTACTCAGCATCGCCAGCAACTTAATCAGTGGACGCAGAAAGTTGACATTGCCCTGAACAGTTGGCGAG‑AAAAAAACAACGTTTGCAGGCCTGGc                                                                       >  1:1862032/1‑100 (MQ=255)

                          gCCATTACTCAGCATCGCCAGCAACTTAATCAGTGGACGCAGAAAGTTGACATTGCCCTGAACAGTTGGCGAG‑AAAAAAACAACGTTTGCAGGCCTGGCa                                                                      <  1:1104353/100‑1 (MQ=255)

                          gCCATTACTCAGCATCGCCAGCAACTTAATCAGTGGACGCAGAAAGTTGACATTGCCCTGAACAGTTGGCGAG‑AAAAAAACAACGTTTGCAGGCCTGGCa                                                                      <  2:1529136/100‑1 (MQ=255)

                                aCTCAGCATCGCCAGCAACTTAATCAGTGGACGCAGAAAGTTGACATTGCCCTGAACAGTTGGCGAG‑AAAAAAACAACGTTTGCAGGCCTGGCAGACACt                                                                <  1:133782/100‑1 (MQ=255)

                                 cTCAGCATCGCCAGCAACTTAATCAGTGGACGCAGAAAGTTGACATTGCCCTGAACAGTTGGCGAG‑AAAAAAACAACGTTTGCAGGCCTGGCAGACACt                                                                >  2:2375208/1‑99 (MQ=255)

                                 cTCAGCATCGCCAGCAACTTAATCAGTGGACGCAGAAAGTTGACATTGCCCTGAACAGTTGGCGAG‑AAAAAAACAACGTTTGCAGGCCTGGCAGACACTg                                                               >  2:2328725/1‑100 (MQ=255)

                                 cTCAGCATCGCCAGCAACTTAATCAGTGGACGCAGAAAGTTGACATTGCCCTGAACAGTTGGCGAG‑AAAAAAACAACGTTTGCAGGCCTGGCAGACACTg                                                               >  2:1706699/1‑100 (MQ=255)

                                  tCAGCATCGCCAGCAACTTAATCAGTGGACGCAGAAAGTTGACATTGCCCTGAACAGTAAGCGAG‑AAAAAAACAACGTTTGCAGGCCTGGCAGACACTGc                                                              <  2:969976/100‑1 (MQ=255)

                                   cAGCATCGCCAGCAACTTAATCAGTGGACGCAGAAAGTTGACATTGCCCTGAACAGTTGGCGAG‑AAAAAAACAACGTTTGCAGGCCTGGCAGACACTGCa                                                             <  1:1481652/100‑1 (MQ=255)

                                   cAGCATCGCCAGCAACTTAATCAGTGGACGCAGAAAGTTGACATTGCCCTGAACAGTTGGCGAG‑AAAAAAACAACGTTTGCAGGCCTGGCAGACACTGCa                                                             >  2:2311160/1‑100 (MQ=255)

                                    aGCATCGCCAGCAACTTAATCAGTGGACGCAGAAAGTTGACATTGCCCTGAACAGTTGGCGAG‑AAAAAAACAACGTTTGCAGGCCTGGCAGACACTGCAg                                                            <  2:2914957/100‑1 (MQ=255)

                                    aGCATCGCCAGCAACTTAATCAGTGGACGCAGAAAGTTGACATTGCCCTGAACAGAAAG‑AAAAAAAAAAACAACGTTTGCAGGCCTGGCAGacac                                                                 <  2:340485/95‑1 (MQ=255)

                                    aGCATCGCCAGCAACTTAATCAATGGACGCAGAAAGTTGACATTGCCCTGAACAGTTGGCGAG‑AAAAAAACAACGTTTGCATGCCTGGCAGACACTGCAg                                                            <  2:2708598/100‑1 (MQ=255)

                                     gCATCGCCAGCAACTTAATCAGTGGACGCAGAAAGTTGACATTGCCCTGAACAGTTGGCGAG‑AAAAAAACAACGTTTGCAGGCCTGGCAGACACTGCAgg                                                           <  1:1122032/100‑1 (MQ=255)

                                      cATCGCCAGCAACTTAATCAGTGGACGCAGAAAGTTGACATTGCCCTGAACAGTTGGCGAG‑AAAAAAACAACGTTTGCAGGCCTGGCAGACACTGCAGGa                                                          >  2:1264607/1‑100 (MQ=255)

                                        tCGCCAGCAACTTAATCAGTGGACGCAGAAAGTTGACATTGCCCTGAACAGTTGGCGAG‑AAAAAAACAACGTTTGCAGGCCTGGCAGACACTGCAGGAAc                                                        >  2:2218370/1‑100 (MQ=255)

                                         cGCCAGCAACTTAATCAGTGGACGCAGAAAGTTGACATTGTCCTGAACAGTTAGAAAG‑AAAAAAACAACGTTTGCAGGCCTGGCAGACACTGCAGGAACg                                                       <  2:35763/100‑1 (MQ=255)

                                         cGCCAGCAACTTAATCAGTGGACGCAGAAAGTTGACATTGCCCTGAACAGTTGGCGAG‑AAAATAACAACGTTTGCAGGCCTGGCAGACACTGCAGGAACg                                                       <  2:1743642/100‑1 (MQ=255)

                                         cGCCAGCAACTTAATCAGTGGACGCAGAAAGTTGACATTGCCCTGAACAGTTGGCGAG‑AAAAAAACAACGTTTGCAGGCCTGGCAGACACTGCAGGAACg                                                       <  1:1407447/100‑1 (MQ=255)

                                         cGCCAGCAACTTAATCAGTGGACGCAGAAAGTTGACATTGCCCTGAACAGTTGGCGAG‑AAAAAAACAACGTTTGCAGGCCTGGCAGACACTGCAGGAACg                                                       >  2:2950901/1‑100 (MQ=255)

                                           ccAGCAACTTAATCAGTGGACGCAGAAAGTTGACATTGCCCTGAACAGTTGGCGAG‑AAAAAAACAACGTTTGCAGGCCTGGCAGACACTGCAGGAACGGc                                                     <  2:683050/100‑1 (MQ=255)

                                           ccAGCAACTTAATCAGTGGACGCAGAAAGTTGACATTGCCCTGAACAGTTGGCGAG‑AAAAAAACAACGTTTGCAGGCCTGGCAGACACTGCAGGAACGGc                                                     >  2:804372/1‑100 (MQ=255)

                                           ccAGCAACTTAATCAGTGGACGCAGAAAGTTGACATTGCCCTGAACAGTTGGCGAG‑AAAAAAACAACGTTTGCAGGCCTGGCAGACACTGCAGGAACGGc                                                     <  1:1684139/100‑1 (MQ=255)

                                           ccAGCAACTTAATCAGTGGACGCAGAAAGTTGACATTGCCCTGAACAGTTGGCGAG‑AAAAAAACAACGTTTGCAGGCCTGGCAGACACTGCAGGAACGGc                                                     >  1:1887899/1‑100 (MQ=255)

                                           ccAGCAACTTAATCAGTGGACGCAGAAAGTTGACATTGCCCTGAACAGTTAACGAG‑AAAAAAACAACGTTTGCAGGCCTGGCAGACACTGCAGGAACGGc                                                     <  2:2583198/100‑1 (MQ=255)

                                            cAGCAACTTAATCAGTGGACGCAGAAAGTTGACATTGCCCTGAACAGCTGGCGAGAAAAGAAACAACGTTTGCAGGCCTGGCAGACACTGCAGGAACGGc                                                     <  2:1968553/100‑1 (MQ=255)

                                             aGCAACTTAATCAGTGGACGCAGAAAGTTGACATTGCCCTGAACAGTTGGCGAG‑AAAAAAACAACGTTTGCAGGCCTGGCAGACACTGCAGGAACGGCaa                                                   >  2:1312951/1‑100 (MQ=255)

                                               cAACTTAATCAGTGGACGCAGAAAGTTGACATTGCCCTGAACAGTTGGCGAG‑AAAAAAACAACGTTTGCAGGCCTGGCAGACACTGCAGGAACGGCAATc                                                 <  1:1858406/100‑1 (MQ=255)

                                               cAACTTAATCAGTGGACGCAGAAAGTTGACATTGCCCTGAACAGTTGGCGAG‑AAAAAAACAACGTTTGCAGGCCTGGCAGACACTGCAGGAACGGCAATc                                                 <  1:1195391/100‑1 (MQ=255)

                                               cAACTTAATCAGTGGACGCAGAAAGTTGACATTGCCCTGAACAGTTGGCGAG‑AAAAAAACAACGTTTGCAGGCCTGGCAGACACTGCAGGAACGGCAATc                                                 <  1:1195142/100‑1 (MQ=255)

                                                accTTAATCAGCGTACGCAGAAAATTGACATTGTCCTGAACAAATAAAAAG‑AAAAAAACAACGTTTGCAGGCCTGGCAGACACTGCAGGAACg                                                       <  2:2378243/91‑1 (MQ=255)

                                                     aaTCAGTGGACGCAGAAAGTTGACATTGCCCTGAACAGTTGGCGAG‑AAAAAAACAACGTTTGCAGGCCTGGCAGACACTGCAGGAACGGCAATCCAcggc                                           <  1:646239/100‑1 (MQ=255)

                                                       tCAGTGGACGCAGAAAGTTGACATTGCCCTGAACAGTTGGCGAG‑AAAAAAACAACGTTTGCAGGCCTGGCAGACACTGCAGGAACGGCAATCCAcggcgg                                         <  2:669566/100‑1 (MQ=255)

                                                        cAGTGGACGCAGAAAGTTGACATTGCCCTGAACAGTTGGCGAG‑AAAAAAACAACGTTTGCAGGCCTGGCAGACACTGCAGGAACGGCAATCCAcggcgg                                         <  1:44519/99‑1 (MQ=255)

                                                        cAGTGGACGCAGAAAGTTGACATTGCCCTGAACAGTTGGCGAG‑AAAAAAACAACGTTTGCAGGCCTGGCAGACACTGCAGGAACGGCAATCCACggcggc                                        <  2:574502/100‑1 (MQ=255)

                                                        cAGTGGACGCAAAAACTTGACATGGCTCTGAACAGTAGGCGAG‑AAAAAAACACCGTTTGCAGGCCTGGCAGACACTGCAGGAACGGCAATCCACggcggc                                        <  2:1012993/100‑1 (MQ=255)

                                                         aGTGGACGCAGAAAGTTGACATTGCCCTGAACAGTTGGCGAG‑AAAAAAACAACGTTTGCAGGCCTGGCAGACACTGCAGGAACGGCAATCCACGGCGGCa                                       >  1:2483495/1‑100 (MQ=255)

                                                            ggACGCAGAAATTTGACATTGCCCTGAACAGTTGGCGAG‑AAAAAAACAACGTTTGCAGGCCTGGCAGACACTGCAGGAACGGCAATCCACGGCGGCACTg                                    <  2:1607892/100‑1 (MQ=255)

                                                            ggACGCAGAAAGTTGACATTGCGCTGAACAGTTGGCGAG‑AAAAAAACAACGTTTGCAGGCCTGGCAGACACTGCAGGAACGGCAATCCACGGCGGCACTg                                    >  2:1898125/1‑100 (MQ=255)

                                                             gACGCAGAAAGTTGACATTGCCCTGAACAGTTGGCGAG‑AAAAAAACAACGTTTGCAGGCCTGGCAGACACTGCAGGAACGGCAATCCACGGCGGCACTGc                                   <  1:2480771/100‑1 (MQ=255)

                                                               cGCAGAAAGTTGACATTGCCCTGAACAGTTGGCGAG‑AAAAAAACAACGTTTGCAGGCCTGGCAGACACTGCAGGAACGGCAATCCACGGCGGCACTGCtt                                 >  1:3148469/1‑100 (MQ=255)

                                                                 cAGAAAGTTGACATTGCCCTGAACAGTTGGCGAG‑AAAAAAACAACGTTTGCAGGCCTGGCAGACACTGCAGGAACGGCAATCCACGGCGGCACTGCTTg                                <  1:2928014/99‑1 (MQ=255)

                                                                 cAGAAAGTTGACATTGCCCTGAACAGTTGGCGAG‑AAAAAAACAACGTTTGCAGGCCTGGCAGACACTGCAGGAACGGCAATCCACGGCGGCACTGCTTGc                               <  2:151745/100‑1 (MQ=255)

                                                                  aGAAAGTTGACATTGCCCTGAACAGTTGGCGAG‑AAAAAAACAACGTTTGCAGGCCTGGCAGACACTGCAGGAACGGCAATCCACGGCGGCACTGCTTGCa                              <  1:2251071/100‑1 (MQ=255)

                                                                  aGAAAGTTGACATTGCCCTGAACAGTTGGCGAG‑AAAAAAACAACGTTTGCAGGCCTGGCAGACACTGCAGGAACGGCAATCCACGGCGGCACTGCTTGCa                              >  1:1004670/1‑100 (MQ=255)

                                                                   gAAAGTTGACATTGCCCTGAACAGTTGGCGAG‑AAAAAAACAACGTTTGCAGGCCTGGCAGACACTGCAGGAACGGCAATCCACGGCGGCACTGCTTGCAg                             <  2:1537224/100‑1 (MQ=255)

                                                                   gAAAGTTGACATTGCCCTGAACAGTTGGCGAG‑AAAAAAACAACGTTTGCAGGCCTGGCAGACACTGCAGGAACGGCAATCCACGGCGGCACTGCTTGCAg                             <  2:1818539/100‑1 (MQ=255)

                                                                    aaaGTTGACATTGCCCTGAACAGTTGGCGAG‑AAAAAAACAACGTTTGCAGGCCTGGCAGACACTGCAGGAACGGCAATCCACGGCGGCACTGCTTGCAGa                            <  1:1006789/100‑1 (MQ=255)

                                                                    aaaGTTGACATTGACCTGAACAGTTGGCGAG‑AAAAAAACAACGTTTGCAGGCCTGGCAGACACTGCAGGAACGGCAATCCACGGCGGCACTGCTTGCAGa                            <  2:462307/100‑1 (MQ=255)

                                                                    aaaGTTGACACTGCCCTGAACAGTTGGCGAG‑AAAAAAACAACGTTTGCAGGCCTGGCAGACACTGCAGGAACGGCAATCCACGGCGGCACTGCTTGCa                              <  2:641193/98‑1 (MQ=255)

                                                                      aGTTGACATTGCCCTGAACAGTTGGCGAG‑AAAAAAACAACGTTTGCAGGCCTGGCAGACACTGCAGGAACGGCAATCCACGGCGGCACTGCTTGCAGaaa                          <  2:257403/100‑1 (MQ=255)

                                                                       gTTGACATTGCCCTGAACAGTTGGCGAG‑AAAAAAACAACGTTTGCAGGCCTGGCAGACACTGCAGGAACGGCAATCCACGGCGGCACTGCTTGCAGaaaa                         >  1:2533503/1‑100 (MQ=255)

                                                                         tGACATTGCCCTGAACAGTTGGCGAG‑AAAAAAACAACGTTTGCAGGCCTGGCAGACACTGCAGGAACGGCAATCCACGGCGGCACTGCTTGCAGAAAAcc                       <  1:2162950/100‑1 (MQ=255)

                                                                          gACATTGCCCTGAACAGTTGGCGAG‑AAAAAAACAACGTTTGCAGGCCTGGCAGACACTGCAGGAACGGCAATCCACGGCGGCACTGCTTGCAGAAAACCg                      <  2:678003/100‑1 (MQ=255)

                                                                          gACATTGCCCTGAACAGTTGGCGAG‑AAAAAAACAACGTTTGCAGGCCTGGCAGACACTGCAGGAACGGCAATCCACGGCGGCACTGCTTGCAGAAAACCg                      <  2:2461892/100‑1 (MQ=255)

                                                                              ttGCCCTGAACAGTTGGCGAG‑AAAAAAACAACGTTTGCAGGCCTGGCAGACACTGCAGGAACGGCAATCCACGGCGGCACTGCTTGCAGAAAACCGCCTc                  <  1:36403/100‑1 (MQ=255)

                                                                               tGCCCTGAACAGTTGGCGAG‑AAAAAAACAACGTTTGCAGGCCTGGCAGACACTGCAGGAACGGCAATCCACGGCGGCACTGCTTGCCGAAAACCGCCttg                 <  1:2218380/100‑3 (MQ=255)

                                                                               tGCCCTGAACAGTTGGCGAG‑AAAAAAACAACGTTTGCAGGCCTGGCAGACACTGCAGGAACGGCAATCCACGGCGGCACTGCTTGCAGAAAACCGCCTCg                 >  1:578019/1‑100 (MQ=255)

                                                                                gCCCTGAACAGTTGGCGAG‑AAAAAAACAACGTTTGCCGGCCTGGCAGACACTGCAGGAACGGCAATCCACGGCGGCACTGCTTGCAGAAAACCGCCTCGa                >  1:2314878/1‑100 (MQ=255)

                                                                                gCCCTGAACAGTTGGCGAG‑AAAAAAACAACGTTTGCAGGCCTGGCAGACACTGCAGGAACGGCAATCCACGGCGGCACTGCTTGCAGAAAACCGCCTCGa                >  1:3154961/1‑100 (MQ=255)

                                                                                 cccTGAACAGTTGGCGAG‑AAAAAAACAACGTTTGCAGGCCTGGCAGACACTGCAGGAACGGCAATCCACGGCGGCACTGCTTGCAGAAAACCGCCTCGAt               >  2:738000/1‑100 (MQ=255)

                                                                                 cccTGAACAGTTGGCGAG‑AAAAAAACAACGTTTGCAGGCCTGGCAGACACTGCAGGAACGGCAATCCACGGCGGCACTGCTTGCAGAAAACCGCCTCGAt               >  1:1529048/1‑100 (MQ=255)

                                                                                 cccTGAACAGTTGGCGAG‑AAAAAAACAACGTTTGCAGGCCTGGCAGACACTGCAGGAACGGCAATCCACGGCGGCACTGCTTGCAGAAAACCGCCTCGAt               <  1:1898131/100‑1 (MQ=255)

                                                                                  ccTGAACAGTTGGCGAG‑AAAAAAACAACGTTTGCAGGCCTGGCAGACACTGCAGGAACGGCAATCCACGGCGGCACTGCTTGCAGAAAACCGCCTCGATc              <  2:2963585/100‑1 (MQ=255)

                                                                                  ccTGAACAGTTGGCGAG‑AAAAAAACAACGTTTGCAGGCCTGGCAGACACTGCAGGAACGGCAATCCACGGCGGCACTGCTTGCAGAAAACCGCCTCGATc              >  2:3257421/1‑100 (MQ=255)

                                                                                   cTGAACAGTTGGCGAG‑AAAAAAACAACGTTTGCAGGCCTGGCAGACACTGCAGGAACGGCAATCCACGGCGGCACTGCTTGCAGAAAACCGCCTCGATCa             >  1:1435650/1‑100 (MQ=255)

                                                                                     gAACAGTTGGCGAG‑AAAAAAACAACGTTTGCAGGCCTGGCAGACACTGCAGGAACGGCAATCCACGGCGGCACTGCTTGCAGAAAACCGCCTCGATCAGa           >  2:2866728/1‑100 (MQ=255)

                                                                                        cAGTTGGCGAG‑AAAAAAACAACGTTTGCAGGCCTGGCAGACACTGCAGGAACGGCAATCCACGGCGGCACTGCTTGCAGAAAACCGCCTCGATCAGaaaa        >  1:2443638/1‑100 (MQ=255)

                                                                                        cAGTTGGCGAG‑AAAAAAACAACGTTTGCAGGCCTGGCAGACACTGCAGGAACGGCAATCCACGGCGGCACTGCTTGCAGAAAACCGCCTCGATCAGaaaa        <  2:558167/100‑1 (MQ=255)

                                                                                          gTTGGCGAGAAAAAAAACAACGTTTGCAGGCCTGGCAGACACTGCAGGAACGGCAATCCACGGCGGCACTGCTTGCAGAAAACCGCCTCGATCAGaaaaa       <  1:2488279/100‑1 (MQ=255)

                                                                                          gTTGGCGAG‑AAAAAAACAACGTTTGCAGGCCTGGCAGACACTGCAGGAACGGCAATCCACGGCGGCACTGCTTGCAGAAAACCGCCTCGATCAGAAAAAg      <  2:1473026/100‑1 (MQ=255)

                                                                                           ttGGCGAG‑AAAAAAACAACGTTTGCAGGCCTGGCAGACACTGCAGGAACGGCAATCCACGGCGGCACTGCTTGCAGAAAACCGCCTCGATCAGAAAAAga     <  1:804374/100‑1 (MQ=255)

                                                                                            tGGCGAG‑AAAAAAACAACGTTTGCAGGCCTGGCAGACACTGCAGGAACGGCAATCCACGGCGGCACTGCTTGCAGAAAACCGCCTCGATCAGAAAAAgat    >  1:2247979/1‑100 (MQ=255)

                                                                                            tGGCGAG‑AAAAAAACAACGTTTGCAGGCCTGGCAGACACTGCAGGAACGGCAATCCACGGCGGCACTGCTTGCAGAAAACCGCCTCGATCAGAAAAAgat    >  1:2347291/1‑100 (MQ=255)

                                                                                            tGGCGAG‑AAAAAAACAACGTTTGCAGGCCTGGCAGACACTGCAGGAACGGCAATCCACGGCGGCACTGCTTGCAGAAAACCGCCTCGATCAGAAAAAgat    <  1:627784/100‑1 (MQ=255)

                                                                                             ggCGAG‑AAAAAAACAACGTTTGCAGGCCTGGCAGACACTGCAGGAACGGCAATCCACGGCGGCACTGCTTGCAGAAAACCGCCTCGATCAGAAAAAgatg   <  1:2105800/100‑1 (MQ=255)

                                                                                             ggCGAG‑AAAAAAACAACGTTTGCAGGCCTGGCAGACACTGCAGGAACGGCAATCCACGGCGGCACTGCTTGCAGAAAACCGCCTCGATCAGAAAAAgatg   >  1:3128403/1‑100 (MQ=255)

                                                                                             ggCGAG‑AAAAAAACAACGTTTGCAGGCCTGGCAGACACTGCAGGAACGGCAATCCACGGCGGCACTGCTTGCAGAAAACCGCCTCGATCAGAAAAAgatg   >  1:385576/1‑100 (MQ=255)

                                                                                              gCGAG‑AAAAAAACAACGTTTGCAGGCCTGGCAGACACTGCAGGAACGGCAATCCACGGCGGCGCTGCTTGCAGAAAACCGCCTCGATCAGAAAAAgatgg  <  2:1754254/100‑1 (MQ=255)

                                                                                              gCGAG‑AAAAAAACAACGTTTGCAGGCCTGGCAGACACTGCAGGAACGGCAATCCACGGCGGCACTGCTTGCAGAAAACCGCCTCGATCAGAAAAAgatgg  <  1:1810851/100‑1 (MQ=255)

AGCAGTTTATCCAGACGCTGGAAAAAGCCATTACTCAGCATCGCCAGCAACTTAATCAGTGGACGCAGAAAGTTGACATTGCCCTGAACAGTTGGCGAGAAAAAAAACAACGTTTGCAGGCCTGGCAGACACTGCAGGAACGGCAATCCACGGCGGCACTGCTTGCAGAAAACCGCCTCGATCAGAAAAAGATGG  >  NC_000913/2018154‑2018348

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`