BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	953,802	A→G	100%	V222A (GTA→GCA)	focA ←	formate channel

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	953,802	0	A	G	99.2%	448.5 / ‑4.6	126	V222A (GTA→GCA)	focA	formate channel
Reads supporting (aligned to +/- strand): ref base A (0/1); new base G (60/65); total (60/66)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.85e-01

Only 100 of 126 total aligned reads displayed.

TTATCAGTGATGAAATTCATCACGGTCAGGTGAGAAAAATTTTCCGGTGCAGAACCGACTGCGGTCCAAAATTCCGGGGATGCGAAGTCGCGGATTACAATACCCATCGGGATCATAAACATGTTTGCGATACTGTGCTCAAAACCGCTGGCAACAAACATCGCGACCGGCAGCACCATAATGAACGCTTTG  >  NC_000913/953706‑953897

ttATCAGTGATGAAATTCATCACGGTCAGGTGAGAAAAATTTTCCGGTGCAGAACCGACTGCGGTCCAAAATTCCGGGGATGCGAAGTCGCGGATTGCaa                                                                                              <  1:3083908/100‑1 (MQ=255)

  gaccgTGATGAATTTCATCACGGTCAGGTGAGAAAAATTTTCCGGTGCAGAACCGACTGCGGTCCAAAATTCCGGGGATGCGAAGTCGCGGATTGCAATa                                                                                            >  1:3070027/5‑100 (MQ=255)

  aTCAGTGATGAAATTCATCACGGTCAGGTGAGAAAAATTTTCCGGTGCAGAACCGACTGCGGTCCAAAATTCCGGGGATGCGAAGTCGCGGATTGCAATa                                                                                            <  2:2324517/100‑1 (MQ=255)

   tCAGTGATGAAATTCATCACGGTCAGGTGAGAAAAATTTTCCGGTGCAGAACCGACTGCGGTCCAAAATTCCGGGGATGCGAAGTCGCGGATTGCAATAc                                                                                           <  1:1017973/100‑1 (MQ=255)

   tCAGTGATGAAATTCATCACGGTCAGGTGAGAAAAATTTTCCGGTGCAGAACCGACTGCGGTCCAAAATTCCGGGGATGCGAAGTCGCGGATTGCAATAc                                                                                           <  1:1153974/100‑1 (MQ=255)

    cAGTGATGAAATTCATCACGGTCAGGTGAGAAAAATTTTCCGGTGCAGAACCGATTGCGGTCCAAAATTCCGGGGATGCGAAGTCGCGGATTGCAATAcc                                                                                          <  1:46879/100‑1 (MQ=255)

    cAGTGATGAAATTCATCACGGTCAGGTGAGAAAAATTTTCCGGTGCAGAACCGACTGCGGTCCAAAATTCCGGGGATGCGAAGTCGCGGATTGCAATAcc                                                                                          >  1:294853/1‑100 (MQ=255)

      gTGATGAAATTCATCACGGTCAGGTGAGAAAAATTTTCCGGTGCAGAACCGACTGCGGTCCAAAATTCCGGGGATGCGAAGTCGCGGATTGCAATACCCa                                                                                        >  2:2398718/1‑100 (MQ=255)

       tgatgaAATTCATCACGGTCAGGTGAGAAAAATTTTCCGGTGCAGAACCGACTGCGGTCCAAAATTCCGGGGATGCGAAGTCGCGGATTGCAATACCCAt                                                                                       <  1:2957961/100‑1 (MQ=255)

       tgatgaAATTCATCACGGTCAGGTGAGAAAAATTTTCCGGTGCAGAACCGACTGCGGTCCAAAATTCCGGGGATGCGAAGTCGCGGATTGCAATACCCAt                                                                                       >  2:1515465/1‑100 (MQ=255)

        gatgaAATTCATCACGGTCAGGTGAGAAAAATTTTCCGGTGCAGAACCGACTGCGGTCCAAAATTCCGGGGATGCGAAGTCGCGGATTGCAATACCCATc                                                                                      >  2:196370/1‑100 (MQ=255)

         atgaAATTCATCACGGTCAGGTGAGAAAAATTTTCCGGTGCAGAACCGACTGCGGTCCAAAATTCCGGGGATGCGAAGTCGCGGATTGCAATACCCATCg                                                                                     <  1:1386243/100‑1 (MQ=255)

          tgaAATTCATCACGGTCAGGTGAGAAAAATTTTCCGGTGCAGAACCGACTGCGGTCCAAAATTCCGGGGATGCGAAGTCGCGGATTGCAATACCCATCgg                                                                                    <  1:778323/100‑1 (MQ=255)

            aaaTTCATCACGGTCAGGTGAGAAAAATTTTCCGGTGCAGAACCGACTGCGGTCCAAAATTCCGGGGATGCGAAGTCGCGGATTGCAATACCCATCGGGa                                                                                  >  1:1782832/1‑100 (MQ=255)

             aaTTCATCACGGTCAGGTGAGAAAAATTTTCCGGTGCAGAACCGACTGCGGTCCAAAATTCCGGGGATGCGAAGTCGCGGATTGCAATACCCATCGGGAt                                                                                 >  1:2740088/1‑100 (MQ=255)

              aTTCATCACGGTCAGGTGAGAAAAATTTTCCGGTGCAGAACCGACTGCGGTCCAAAATTCCGGGGATGCGAAGTCGCGGATTGCAATACCCATCGGGATc                                                                                <  1:1510341/100‑1 (MQ=255)

                  atcaCGGTCAGGTGAGAAAAATTTTCCGGTGCAGAACCGACTGCGGTCCAAAATTCCGGGGATGCGAAGTCGCGGATTGCAATACCCATCGGGATCATaa                                                                            >  1:1558086/1‑100 (MQ=255)

                  atcaCGGTCAGGTGAGAAAAATTTTCCGGTGCAGAACCGACTGCGGTCCAAAATTCCGGGGATGCGAAGTCGCGGATTGCAATACCCATCGGGATCATaa                                                                            >  1:1756523/1‑100 (MQ=255)

                  atcaCGGTCAGGTGAGAAAAATTTTCCGGTGCAGAACCGACTGCGGTCCAAAATTCCGGGGATGCGAAGTCGCGGATTGCAATACCCATCGGGATCATaa                                                                            >  2:1385971/1‑100 (MQ=255)

                    catggTCAGGTGAGAAAAATTTTCCGGTGCAGAACCGACTGCGGTCCAAAATTCCGGGGATGCGAAGTCGCGGATTGCAATACCCATCGGGATCATAAAc                                                                          <  1:1631796/97‑1 (MQ=255)

                    caCGGTCAGGTGAGAAAAATTTTCCGGTGCAGAACCGACTGCGGTCCAAAATTCCGGGGATGCGAAGTCGCGGATTGCAATACCCATCGGGATCATAAAc                                                                          >  2:649908/1‑100 (MQ=255)

                    caCGGTCAGGCGAGAAAAATTTTCCGGTGCAGAACCGACTGCGGTCCAAAATTCCGGGGATGCGAATTCTCGGATTGCAATACCCATCGGGATCATAAAc                                                                          <  2:2146646/100‑1 (MQ=255)

                       ggTCAGGTGAGAAAAATTTTCCGGTGCAGAACCGACTGCGGTCCAAAATTCCGGGGATGCGAAGTCGCGGATTGCAATACCCATCGGGATCATAAACATg                                                                       <  1:2049047/100‑1 (MQ=255)

                       ggTCAGGTGAGAAAAATTTTCCGGTGCAGAACCGACTGCGGTCCAAAATTCCGGGGATGCGAAGTCGCGGATTGCAATACCCATCGGGATCATAAACATg                                                                       >  2:1322108/1‑100 (MQ=255)

                        gTCAGGTGAGAAAAATTTTCCGGTGCAGAACCGACTGCGGTCCAAAATTCCGGGGATGCGAAGTCGCGGATTGCAATACCCATCGGGATCATAAACATGt                                                                      >  1:1930818/1‑100 (MQ=255)

                          cAGGTGAGAACAATTTTCCGGTGCAGAACCGACTGCGGTCCAAAATTCCGGGGATGCGAAGTCGCGGATTGCAATACCCATCGGGATCATAAACATGttt                                                                    >  2:2305077/1‑100 (MQ=255)

                          cAGGTGAGAAAAATTTTCCGGTGCAGAACCGACTGCGGTCCAAAATTCCGGGGATGCGAAGTCGCGGATTGCAATACCCATCGGGATCATAAACATGttt                                                                    >  2:2126254/1‑100 (MQ=255)

                           aGGTGAGAAAAATTTTCCGGTGCAGAACCGACTGCGGTCCAAAATTCCGGGGATGCGAAGTCGCGGATTGCAATACCCATCGGGATCATAAACATGTTTg                                                                   <  1:710946/100‑1 (MQ=255)

                            ggTGAGAAAAATTTTCCGGTGCAGAACCGACTGCGGTCCAAAATTCCGGGGATGCGAAGTCGCGGATTGCAATACCCATCGGGATCATAAACATGTTTGc                                                                  >  2:2108121/1‑100 (MQ=255)

                            ggTGAGAAAAATTTTCCGGTGCAGAACCGACTGCGGTCCAAAATTCCGGGGATGCGAAGTCGCGGATTGCAATACCCATCGGGATCATAAACATGTTTGc                                                                  <  1:2268615/100‑1 (MQ=255)

                            ggTGAGAAAAATTTTCCGGTGCAGAACCGACTGCGGTCCAAAATTCCGGGGATGCGAAGTCGCGGATTGCAATACCCATCGGGATCATAAACATGTTTGc                                                                  <  2:780356/100‑1 (MQ=255)

                                 gaAAAATTTTCCGGTGCAGAACCGACTGCGGTCCAAAATTCCGGGGATGCGAAGTCGCGGATTGCAATACCCCTCGGGATCATAAACATGTTTGCGATAc                                                             >  1:887937/1‑100 (MQ=255)

                                 gaAAAATTTTCCGGTGCAGAACCGACTGCGGTCCAAAATTCCGGGGATGCGAAGTCGCGGATTGCAATACCCATCGGGATCATAAACATGTTTGCGATAc                                                             >  1:2141169/1‑100 (MQ=255)

                                 gaAAAATTTTCCGGTGCAGAACCGACTGCGGTCCAAAATTCCGGGGATGCGAAGTCGCGGATTGCAATACCCATCGGGATCATAAACATGTTTGCGATAc                                                             >  1:3154031/1‑100 (MQ=255)

                                 gaAAAATTTTCCGGTGCAGAACCGACTGCGGTCCAAAATTCCGGGGATGCGAAGTCGCGGAATGCAATACCCATCGGGATCATAAACATGTTTGCGATa                                                              >  2:1307172/1‑99 (MQ=255)

                                   aaaaTTTTCCGGTGCAGAACCGACTGCGGTCCAAAATTCCGGGGATGCGAAGTCGCGGATTGCAATACCCATCGGGATCATAAACATGTTTGCGATACtg                                                           <  1:2777634/100‑1 (MQ=255)

                                   aaaaTTTTCCGGTGCAGAACCGACTGCGGTCCAAAATTCCGGGGATGCGAAGTCGCGGATTGCAATACCCATCGGGATCATAAACATGTTTGCGATACtg                                                           <  1:1409384/100‑1 (MQ=255)

                                   aaaaTTTTCCGGTGCAGAACCGACTGCGGTCCAAAATTCCGGGGATGCGAAGTCGCGGATTGCAATACCCATCGGGATCATAAACATGTTTGCGATACtg                                                           >  2:3119911/1‑100 (MQ=255)

                                      aTTTTCCGGTGCAGAACCGACTGCGGTCCAAAATTCCGGGGATGCGAAGTCGCGGATTGCAATACCCATCGGGATCATAAACATGTTTGCGATACTGTGc                                                        <  1:334157/100‑1 (MQ=255)

                                      aTTTTCCGGTGCAGAACCGACTGCGGTCCAAAATTCCGGGGATGCGAAGTCGCGGATTGCAATACCCATCGGGATCATAAACATGTTTGCGATACTGTGc                                                        <  1:668140/100‑1 (MQ=255)

                                      aTTTTCCGGTGCAGAACCGACTGCGGTCCAAAATTCCGGGGATGCGAAGTCGCGGATTGCAATACCCATCGGGATCATAAACATGTTTGCGATACTGTGc                                                        <  1:1239000/100‑1 (MQ=255)

                                      aTTTTCCGGTGCAGAACCGACTGCGGTCCAAAATTCCGGGGATGCGAAGTCGCGGATTGCAATACCCATCGGGATCATAAACATGTTTGCGATACTGTGc                                                        <  2:1259368/100‑1 (MQ=255)

                                      aTTTTCCGGTGCAGAACCGACTGCGGTCCAAAATTCCGGGGATGCGAAGTCGCGGATTGCAATACCCATCGGGATCATAAACATGTTTGCGATACTGTGc                                                        <  1:1874357/100‑1 (MQ=255)

                                         ttCCGGTGCAGAACCGACTGCGGTCCAAAATTCCGGGGATGCGAAGTCGCGGATTGCAATACCCATCGGGATCATAAACATGTTTGCGATACTGTGCTCa                                                     <  2:733583/100‑1 (MQ=255)

                                           ccGGTGCAGATCCGACTGCGGTCCAAAATTCCGGGGATGCGAAGTCGCGGATTGCAATACCCATCGTGATCATAAACATGTTTGCGATACTGTGCTCaaa                                                   <  2:2145477/100‑1 (MQ=255)

                                           ccGGTGCAGAACCGACTGCGGTCCAAAATTCCGGGGATGCGAAGTCGCGGATTGCAATACCCATCGGGATCATAAACATGTTTGCGATACTGTGCTCa                                                     <  1:1008515/98‑1 (MQ=255)

                                            cGGTGCAGAACCGACTGCGGTCCAAAATTCCGGGGATGCGAAGTCGCGGATAGCAATACCCATCGGGATCATAAACATGTTTGCGATACTGTGCTCaaaa                                                  >  1:848516/1‑100 (MQ=255)

                                             ggTGCAGAACCGACTGCGGTCCAAAATTCCGGGGATGCGAAGTCGCGGATTGCAATACCCATCGGGATCATAAACATGTTTGCGATACTGTGCTCAAAAc                                                 <  1:2108128/100‑1 (MQ=255)

                                              gTGCAGAACCGACTGCGGTCCAAAATTCCGGGGATGCGAAGTCGCGGATTGCAATACCCATCGGGATCATAAACATGTTTGCGATACTGTGCTCAAAAcc                                                >  2:17515/1‑100 (MQ=255)

                                              gTGCAGAACCGACTGCGGTCCAAAATTCCGGGGATGCGAAGTCGCGGATTGCAATACCCATCGAGATCATAAACATGTTTGCGATACTGTGCTCAAAAcc                                                <  2:609783/100‑1 (MQ=255)

                                               tGCAGAACCGACTGCGGTCCAAAATTCCGGGGATGCGAAGTCGCGGATTGCAATACCCATCGGGATCATAAACATGTTTGCGATACTGTGCTCAAAACCg                                               >  1:1336378/1‑100 (MQ=255)

                                                  aGAACCGACTGCGGTCCAAAATTCCGGGGATGCGAAGTCGCGGATTGCAATACCCATCGGGATCATAAACATGTTTGCGATACTGTGCTCAAAACCGCTg                                            >  2:2671849/1‑100 (MQ=255)

                                                  aGAACCGACTGCGGTCCAAAATTCCGGGGATGCGAAGTCGCGGATTGCAATACCCATCGGGATCATAAACATGTTTGCGATACTGTGCTCAAAACCGCTg                                            >  2:1840918/1‑100 (MQ=255)

                                                   gAACCGACTGCGGTCCAAAATTCCGGGGATGCGAAGTCGCGGATTGCAATACCCATCGGGATCATAAACATGTTTGCGATACTGTGCTCAAAACCGCTgg                                           <  2:1593051/100‑1 (MQ=255)

                                                   gAACCGACTGCGGTCCAAAATTCCGGGGATGCGAAGTCGCGGATTGCAATACCCATCGGGATCATAAACATGTTTGCGATACTGTGCTCAAAACCGCTgg                                           >  1:917446/1‑100 (MQ=255)

                                                   gAACCGACTGCGGTCCAAAATTCCGGGGATGCGAAGTCGCGGATTGCAATACCCATCGGGATCATAAACATGTTTGCGATACTGTGCTCAAAACCGCTgg                                           <  1:1701617/100‑1 (MQ=255)

                                                   gAACCGACTGCGGTCCAAAATTCCGGGGATGCGAAGTCGCGGATTGCAATACCCATCGGGATCATAAACATGTTTGCGATACTGTGCTCAAAACCGCTgg                                           >  1:1258468/1‑100 (MQ=255)

                                                    aaCCGACTGCGGTCCAAAATTCCGGGGATGCGAAGTCGCGGATTGCAATACCCATCGGGATCATAAACATGTTTGCGATACTGTGCTCAAAACCGCTGGc                                          <  2:3070009/100‑1 (MQ=255)

                                                    aaCCGACTGCGGTCCAAAATTCCGGGGATGCGAAGTCGCGGATTACAATACCCATCGGGATCATAAACATGTTTGCGATACTGTGCTCAAAACCGCTGGc                                          <  1:176159/100‑1 (MQ=255)

                                                     aCCGACTGCGGTCCAAAATTCCGGGGATGCGAAGTCGCGGATTGCGATACCCATCGGGATCATAAACATGTTTGCGATACTGTGCTCAAAACCGCTGGca                                         >  2:3153245/1‑100 (MQ=255)

                                                     aCCGACTGCGGTCCAAAATTCCGGGGATGCGAAGTCGCGGATTGCAATACCCATCGGGATCATAAACATGTTTGCGATACTGTGCTCAAAACCGCTGGca                                         <  1:2186568/100‑1 (MQ=255)

                                                       cGACTGCGGTCCAAAATTCCGGGGATGCGAAGTCGCGGATTGCAATACCCATCGGGATCATAAACATGTTTGCGATACTGTGCTCAAAACCGCTGGcaac                                       >  2:1153736/1‑100 (MQ=255)

                                                       cGACTGCGGTCCAAAATTCCGGGGATGCGAAGTCGCGGATTGCAATACCCATCGGGATCATAAACATGTTTGCGATACTGTGCTCAAAACCGCTGGcaac                                       <  2:84796/100‑1 (MQ=255)

                                                        gACTGCGGTCCAAAATTCCGGGGATGCGAAGTCGCGGATTGCAATACCCATCGGGATCATAAACATGTTTGCGATACTGTGCTCAAAACCGCTGGcaaca                                      >  1:603731/1‑100 (MQ=255)

                                                        gACTGCGGTCCAAAATTCCGGGGATGCGAAGTCGCGGATTGCAATACCCATCGGGATCATAAACATGTTTGCGATACTGTGCTCAAAACCGCTGGcaaca                                      <  1:1052362/100‑1 (MQ=255)

                                                         aCTGCGGTCCAAAATTCCGGGGATGCGAAGTCGCTGATTGCAATACCCATCGGGATCATAAACATGTTTGCGATACTGTGCTCAAAACCGCTGGcaacaa                                     <  2:1728551/100‑1 (MQ=255)

                                                         aCTGCGGTCCAAAATTCCGGGGATGCGAAGTCGCGGATTGCAATACCCATCGGGATCATAAACATGTTTGCGATACTGTGCTCAAAACCGCTGGcaacaa                                     <  2:2857556/100‑1 (MQ=255)

                                                          cTGCGGTCCAAAATTCCGGGGATGCGAAGTCGCGGGTTGCAATACCCATCGGGGTCATAAAAATGTTTGCGATACTGTGCTCAAAACCGCTGGCaacaaa                                    >  2:2942732/1‑100 (MQ=255)

                                                           tGCGGTCCAAAATTCCGGGGATGCGAAGTCGCGGATTGCAATACCCATCGGGATCATAAACATGTTTGCGATACTGTGCTCAAAACCGCTGGCaacaaac                                   <  1:3047200/100‑1 (MQ=255)

                                                              ggTCCAAAATTCCGGGGATGCGAAGTCGCGGATTGCAATACCCATCGGGATCATAAACATGTTTGCGATACTGTGCTCAAAACCGCTGGCAACAAACATc                                >  1:2089735/1‑100 (MQ=255)

                                                               gTCCAAAATTCCGGGGATGCGAAGTCGCGGATTGCAATACCCATCGGGATCATAAACATGTTTGCGATACTGTGCTCAAAACCGCTGGCAACAAACATcg                               >  2:487100/1‑100 (MQ=255)

                                                               gTCCAAAATTCCGGGGATGCGAAGTCGCGGATTGCAATACCCATCGGGATCATAAACATGTTTGCGATACTGTGCTCAAAACCGCTGGCAACAAACATcg                               >  1:2708629/1‑100 (MQ=255)

                                                                 ccAAAATTCCGGGGATGCGAAGTCGCGGATTGCAATACCCATCGGGATCATAAACATGTTTGCGATACTGTGCTCAAAACCGCTGGCAACAAACATcgcg                             >  1:999172/1‑100 (MQ=255)

                                                                 ccAAAATTCCGGGGATGCGAAGTCGCGGATTGCAATACCCATCGGGATCATAAACATGTTTGCGATACTGTGCTCAAAACCGCTGGCAACAAACATcgcg                             <  1:928199/100‑1 (MQ=255)

                                                                    aaaTTCCGGGGATGCGAAGTCGCGGATTGCAATACCCATCGGGATCATAAACATGTTTGCGATACTGTGCTCAAAACCGCTGGCAACAAACATCGCGAcc                          <  2:3154387/100‑1 (MQ=255)

                                                                       ttCCGGGGATGCGAAGTCGCGGATTGCAATACCCATCGGGATCATAAACATGTTTGCGATACTGTGCTCAAAACCGCTGGCAACAAACATCGCGACCGgc                       <  2:795199/100‑1 (MQ=255)

                                                                       ttCCGGGGATGCGAAGTCGCGGATTGCAATACCCATCGGGATCATAAACATGTTTGCGATACTGTGCTCAAAACCGCTGGCAACAAACATCGCGACCGgc                       <  1:1412894/100‑1 (MQ=255)

                                                                        tCCGGGGATGCGAAGTCGCGGATTGCAATACCCATCGGGATCATAAACATGTTTGCGATACTGTGCTCAAAACCGCTGGCAACAAACATCGCGACCGgca                      <  2:508288/100‑1 (MQ=255)

                                                                        tCCGGGGATGCGAAGTCGCGGATTGCAATACCCATCGGGATCATAAACATGTTTGCGATACTGTGCTCAAAACCGCTGGCAACAAACATCGCGACCGgca                      <  2:3253173/100‑1 (MQ=255)

                                                                          cGGGGATGCGAAGTCGCGGATTGCAATACCCATCGGGATCATAAACATGTTTGCGATACTGTGCTCAAAACCGCTGGCAACAAACATCGCGACCGgcagc                    >  1:1460847/1‑100 (MQ=255)

                                                                          cGGGGATGCGAAGTCGCGGATTGCAATACCCATCGGGATCATAAACATGTTTGCGATACTGTGCTCAAAACCGCTGGCAACAAACATCGCGACCGgcagc                    >  1:2653808/1‑100 (MQ=255)

                                                                          cGGGGATGCGAAGTCGCGGATTGCAATACCCATCGGGATCATAAACATGTTTGCGATACTGTGCTCAAAACCGCTGGCAACAAACATCGCGACCGgcagc                    >  1:1328349/1‑100 (MQ=255)

                                                                           ggggATGCGAAGTCGCGGATTGCAATACCCATCGGGATCATAAACATGTTTGCGATACTGTGCTCAAAACCGCTGGCAACAAACATCGCGACCGgcagca                   <  2:393856/100‑1 (MQ=255)

                                                                           ggggATGCGAAGTCGCGGATTGCAATACCCATCGGGATCATAAACATGTTTGCGATACTGTGCTCAAAACCGCTGGCAACAAACATCGCGACCGgcagca                   <  1:2915118/100‑1 (MQ=255)

                                                                            gggATGCGAAGTCGCGGATTGCAATACCCATCGGGATCATAAACATGTTTGCGATACTGTGCTCAAAACCGCTGGCAACAAACATCGCGACCGGCAGCAc                  <  2:887935/100‑1 (MQ=255)

                                                                             ggATGCGAAGTCGCGGATTGCAATACCCCTCGGGATCATAACCATGTTTGCGATACTGTGCTCAAACCCGCTGGCAACAAACATCGCGACCGGCAGCAcc                 <  2:1968790/100‑1 (MQ=255)

                                                                             ggATGCGAAGTCGCGGATTGCAATACCCATCGGGATCATAAACATGTTTGCGATACTGTGCTCAAAACCGCTGGCAACAAACATCGCGACCGGCAGCAcc                 <  2:2515367/100‑1 (MQ=255)

                                                                             ggATGCGAAGTCGCGGATTGCAATACCCATCGGGATCATAAACATGTTTGCGATACTGTGCTCAAAACCGCTGGCAACAAACATCGCGACCGGCAGCAcc                 <  1:424876/100‑1 (MQ=255)

                                                                                tGCGAAGTCGCGGATTGCAATACCCATCGGGATCATAACCATGTTTGCGATACTGTGCACAAAACCGCTGGCAACAAACATCGCGACCGGCAGCACCATa              <  2:1782827/100‑1 (MQ=255)

                                                                                tGCGAAGTCGCGGATTGCAATACCCATCGGGATCATAAACATGTTTGCGATACTGTGCTCAAAACCGCTGGCAACAAACATCGCGACCGGCAGCACCATa              >  1:2559385/1‑100 (MQ=255)

                                                                                 gCGAAGTCGCGGATTGCAATACCCATCGGGATCATAAACATGTTTGCGATACTGTGCTCAAAACCGCTGGCAACAAACATCGCGACCGGCAGCACCATaa             >  2:1071030/1‑100 (MQ=255)

                                                                                   gAAGTCGCGGATTGCAATACCCATCGGGATCATAAACATGTTTGCGATACTGTGCTCAAAACCGCTGGCAACAAACATCGCGACCGGCAGCACCATAATg           >  1:2085590/1‑100 (MQ=255)

                                                                                    aaGTCGCGGATTGCAATACCCATCGGGATCATAAACATGTTTGCGATACTGTGCTCAAAACCGCTGGCAACAAACATCGCGACCGGCAGCACCATAATGa          <  2:3007793/100‑1 (MQ=255)

                                                                                      gTCGCGGATTGCAATACCCATCGGGATCATAAACATGTTTGCGATACTGTGCTCAAAACCGCTGGCAACAAACATCGCGACCGGCAGCACCATAATGAAc        >  2:2859658/1‑100 (MQ=255)

                                                                                      gTCGCGGATTGCAATACCCATCGGGATCATAAACATGTTTGCGATACTGTGCTCAAAACCGCTGGCAACAAACATCGCGACCGGCAGCACCATAATGAAc        >  2:2398571/1‑100 (MQ=255)

                                                                                      gTCGCGGATTGCAATACCCATCGGGATCATAAACATGTTTGCGATACTGTGCTCAAAACCGCTGGCAACAAACATCGCGACCGGCAGCACCATAATGAAc        >  2:1024406/1‑100 (MQ=255)

                                                                                        cgcgGATTGCAATACCCATCGGGATCATAAACATGTTTGCGATACTGTGCTCAAAACCGCTGGCAACAAACATCGCGACCGGCAGCACCATAATGAACGc      >  1:1394280/1‑100 (MQ=255)

                                                                                        cgcgGATTGCAATACCCATCGGGATCATAAACATGTTTGCGATACTGTGCTCAAAACCGCTGGCAACAAACATCGCGACCGGCAGCACCATAATGAACGc      >  2:2273989/1‑100 (MQ=255)

                                                                                        cgcgGATTGCAATACCCATCGGGATCATAAACATGTTTGCGATACTGTGCTCAAAACCGCTGGCAACAAACATCGCGACCGGCAGCACCATAATGAACGc      >  1:1869665/1‑100 (MQ=255)

                                                                                        cgcgGATTGCAATACCCATCGGGATCATAAACATGTTTGCGATACTGTGCTCAAAACCGCTGGCAACAAACATCGCGACCGGCAGCACCATAATGAACGc      >  1:2202396/1‑100 (MQ=255)

TTATCAGTGATGAAATTCATCACGGTCAGGTGAGAAAAATTTTCCGGTGCAGAACCGACTGCGGTCCAAAATTCCGGGGATGCGAAGTCGCGGATTACAATACCCATCGGGATCATAAACATGTTTGCGATACTGTGCTCAAAACCGCTGGCAACAAACATCGCGACCGGCAGCACCATAATGAACGCTTTG  >  NC_000913/953706‑953897

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`