Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,866,592 +C coding (960/993 nt) rpoS ← RNA polymerase, sigma S (sigma 38) factor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,866,5881.C99.1% 430.9 / ‑2.7 116coding (964/993 nt)rpoSRNA polymerase, sigma S (sigma 38) factor
Reads supporting (aligned to +/- strand):  ref base . (1/0);  new base C (62/53);  total (63/53)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.42e-01

Only 100 of 127 total aligned reads displayed.

ATCGGACCTTTTATTGTGCACAGAAAAGGCCAGCCTCGCTTGAGACTGGCCTTTCTGACAGATGCTTACTTACTCGCGGAACAGCGCTTCGATATTCAGCCCCTGCGTTTGCAGGATTTCGCGCAAACGGCGCAGGCCTTCAACCTGAATCTGGCGAACACGTTCACGGGTGAGGCCAATTTCACGACCTACA  >  NC_000913/2866490‑2866682
                                                                                                   |                                                                                              
aTCGGACCTTTTATTGTGCACAGAAAAGGCCAGCCTCGCTTGAGACTGGCCTTTCTGACAGATGCTTACTTACTCGCGGAACAGCGCTTCGATATTCAGc                                                                                               >  1:1894656/1‑100 (MQ=255)
 tCGGACCTTTTATTGTGCACAGAAAAGGCCAGCCTCGCTTGAGACTGGCCTTTCTGACAGATGCTTACTTACTCGCGGAACAGCGCTTCGATATTCAGcc                                                                                              >  2:272582/1‑100 (MQ=255)
  cGGACCTTTTATTGTGCACAGAAAAGGCCAGCCTCGCTTGAGACTGGCCTTTCTGACAGATGCTTACTTACTCGCGGAACAGCGCTTCGATATTCAGccc                                                                                             <  1:2985350/100‑1 (MQ=255)
  cGGACCTTTTATTGTGCACAGAAAAGGCCAGCCTCGCTTGAGACTGGCCTTTCTGACAGATGCTTACTTACTCGCGGAACAGCGCTTCGATATTCAGccc                                                                                             >  2:1669343/1‑100 (MQ=255)
   ggACCTTTTATTGTGCACAGAAAAGGCCAGCCTCGCTTGAGACTGGCCTTTCTGACAGATGCTTACTTACTCGCGGAACAGCGCTTCGATATTCAGcccc                                                                                            >  2:1469240/1‑100 (MQ=255)
     aCCTTTTATTGTGCACAGAAAAGGCCAGCCTCGCTTGAGACTGGCCTTTCTGACAGATGCTTACTTACTCGCGGAACAGCGCTTCGATATTCAGccccc                                                                                           <  2:934173/99‑2 (MQ=255)
      ccTTTTATTGTGCACAGAAAAGGCCAGCCTCGCTTGAGACTGGCCTTTCTGACAGATGCTTACTTACTCGCGGAACAGCGCTTCGATATTCAGccccctg                                                                                         <  1:1275139/100‑4 (MQ=255)
      ccTTTTATTGTGCACAGAAAAGGCCAGCCTCGCTTGAGACTGGCCTTTCTGACAGATGCTTACTTACTCGCGGAACAGCGCTTCGATATTCAGccccc                                                                                           >  1:720/1‑97 (MQ=255)
        ttttGTTGTGCACAGATTATGCCTTCTCCGCTTGTTATTGGCCTTTCTGTCAGTTGCTTACTTACTCGCGGAACAGCGCTTCGATATTCAGccccctt                                                                                         <  1:487831/98‑4 (MQ=255)
          ttATTGTGCACAGAAAAGGCCAGCCTCGCTTGAGACTGGCCTTTCTGACAGATGCTTACTTACTCGCGGAACAGCGCTTCGATATTCAGCCCCCTGCGtt                                                                                      <  1:2537022/100‑1 (MQ=255)
           tATTGTGCACAGAAAAGGCCAGCCTCGCTTGAGACTGGCCTTTCTGACAGATGCTTACTTACTCGCGGAACAGCGCTTCGATATTCAGCCCCCTGCGttt                                                                                     >  2:2643764/1‑100 (MQ=255)
             ttGTGCACAGAAAAGGCCAGCCTCGCTTGAGACTGGCCTTTCTGACAGATGCTTACTTACTCGCGGAACAGCGCTTCGATATTCAGCCCCCTGCGTTTGc                                                                                   <  2:2637483/100‑1 (MQ=255)
              tgtgCACAGAAAAGGCCAGCCTCGCTTGAGACTGGCCTTTCTGACAGATGCTTACTTACTCGCGGAACAGCGCTTCGATATTCAGCCCCCTGCGTTTGCa                                                                                  >  2:1393290/1‑100 (MQ=255)
               gtgCACAGAAAAGGCCAGCCTCGCTTGAGACTGGCCTTTCTGACAGATGCTTACTTACTCGCGGAACAGCGCTTCGATATTCAGCCCCCTGCGTTTGCAg                                                                                 >  1:573290/1‑100 (MQ=255)
               gtgCACAGAAAAGGCCAGCCTCGCTTGAGACTGGCCTTTCTGACAGATGCTTACTTACTCGCGGAACAGCGCTTCGATATTCAGCCCCCTGCGTTTGCAg                                                                                 <  1:278753/100‑1 (MQ=255)
               gtgCACAGAAAAGGCCAGCCTCGCTTGAGACTGGCCTGTCTGACAGATGCTTACTTACGCGCGGAACAGCGCTTCGATATTCAGCCCCCTGCGTTTGCAg                                                                                 >  2:2619589/1‑100 (MQ=255)
                tgCACAGAAAAGGCCAGCCTCGCTTGAGACTGGCCTTTCTGACAGATGCTTACTTACTCGCGGAACAGCGCTTCGATATTCAGCCCCCTGCGTTTGCAgg                                                                                <  2:1894662/100‑1 (MQ=255)
                 gCACAGAAAAGGCCAGCCTCGCTTGAGACTGGCCTTTCTGACAGATGCTTACTTACTCGCGGAACAGCGCTTCGATATTCAGCCCCCTGCGTTTGCAGGa                                                                               >  1:2054276/1‑100 (MQ=255)
                  cacaGAAAAGGCCAGCCTCGCTTGAGACTGGCCTTTCTGACAGATGCTTACTTACTCGCGGAACAGCGCTTCGATATTCAGCCCCCTGCGTTTGCAGGAt                                                                              <  2:453081/100‑1 (MQ=255)
                  cacaGAAAAGGCCAGCCTCGCTTGAGACTGGCCTTTCTGACAGATGCTTACTTACTCGCGGAACAGCGCTTCGATATTCAGCCCCCTGCGTTTGCAGGAt                                                                              >  2:1049458/1‑100 (MQ=255)
                    caGAAAAGGCCAGCCTCGCTTGAGACTGGCCTTTCTGACAGATGCTTACTTACTCGCGGAACAGCGCTTCGATATTCAGCCCCCTGCGTTTGCAGGAttt                                                                            >  2:2959244/1‑100 (MQ=255)
                    caGAAAAGGCCAGCCTCGCTTGAGACTGGCCTTTCTGACAGATGCGTACTTACTCGCGGAACAGCGCTTCGATATTCAGCCCCCTGCGTTTGCAGGAttt                                                                            >  1:3053141/1‑100 (MQ=255)
                     aGAAAAGGCCAGCCTCGCTTGAGACTGGCCTTTCTGACAGATGCTTACTTACTCGCGGAACAGCGCTTCGATATTCAGCCCCCTGCGTTTGCAGGATTTc                                                                           >  1:2900083/1‑100 (MQ=255)
                     aGAAAAGGCCAGCCTCGCTTGAGACTGGCCTTTCTGACAGATGCTTACTTACTCGCGGAACAGCGCTTCGATATTCAGCCCCCTGCGTTTGCAGGATTTc                                                                           >  1:2160563/1‑100 (MQ=255)
                           ggCCAGCCTCGCTTGAGACTGGCCTTTCTGACAGATGCTTACTTACTCGCGGAACAGCGCTTCGATATTCAGCCCCCTGCGTTTGCAGGATTTCGCGCaa                                                                     >  1:728899/1‑100 (MQ=255)
                           ggCCAGCCTCGCTTGAGACTGGCCTTTCTGACAGATGCTTACTTACTCGCGGAACAGCGCTTCGATATTCAGCCCCCTGCGTTTGCAGGATTTCGCGCaa                                                                     >  2:2996857/1‑100 (MQ=255)
                            gCCAGCCTCGCTTGAGACTGGCCTTTCTGACAGATGCTTACTTACTCGCGGAACAGCGCTTCGATATTCAGCCCCCTGCGTTTGCAGGATTTCGCGCaaa                                                                    >  2:2629540/1‑100 (MQ=255)
                             ccAGCCTCGCTCGAGACTGGCCTTTCTGACAGATGCTTACTTACTCGCGGAACAGCGCTTCGATATTCAGCCCCCTGCGTTTGCAGGATTTCGCGCAAAc                                                                   >  1:1608824/1‑100 (MQ=255)
                                gCCTCGCTTGAGACTGGCCTTTCTGACAGATGCTTACTTACTCGCGGAACAGCGCTTCGATATTCAGCCCCCTGCGTTTGCAGGATTTCGCGCAAACGgc                                                                >  1:278837/1‑100 (MQ=255)
                                   tCGCTTGAGACTGGCCTTTCTGACAGATGCTTACTTACTCGCGGAACAGCGCTTCGATATTCAGCCCCCTGCGTTTGCAGGATTTCGCGCAAACGGCGCa                                                             <  1:1324112/100‑1 (MQ=255)
                                    cGCTTGAGACTGGCCTTTCTGACAGATGCTTACTTACTCGCGGAACAGCGCTTCGATATTCAGCCCCCTGCGTTTGCAGGATTTCGCGCAAACGGCGCAg                                                            >  2:490851/1‑100 (MQ=255)
                                    cGCTTGAGACTGGCCTTTCTGACAGATGCTTACTTACTCGCGGAACAGCGCTTCGATATTCAGCCCCCTGCGTTTGCAGGATTTCGCGCAAACGGCGCAg                                                            <  2:2733503/100‑1 (MQ=255)
                                       ttGAGACTGGCCTTTCTGACAGATGCTTACTTACTCGCGGAACAGCGCTTCGATATTCAGCCCCCTGCGTTTGCAGGATTTCGCGCAAACGGCGCAGGcc                                                         <  2:847605/100‑1 (MQ=255)
                                        tGAGACTGGCCTTTCTGACAGATGCTTACTTACTCGCGGAACAGCGCTTCGATATTCAGCCCCCTGCGCTTGCAGGCTTTCGCGCAAACGGCGCAGCCCt                                                        >  1:2855734/1‑100 (MQ=255)
                                        ngagaCTGGCCTTTCTGACAGATGCTTACTTACTCGCGGAACAGCGCTTCGATATTCAGCCCCCTGCGTTTGCAGGATTTCGCGCAAACGGCGCAGGCCt                                                        >  2:771493/2‑100 (MQ=255)
                                         gagaCTGGCCTTTCTGACAGATGCTTACTTACTCGCGGAACAGCGCTTCGATATTCAGCCCCCTGCGTTTGCAGGATTTCGCGCAAACGGCGCAGGCCtt                                                       >  1:1250847/1‑100 (MQ=255)
                                         gagaCTGGCCTTTCTGACAGATGCTTACTTACTCGCGGAACAGCGCTTCGATATTCAGCCCCCTGCGTTTGCAGGATTTCGCGCAAACGGCGCAGGCCtt                                                       <  2:1116486/100‑1 (MQ=255)
                                          agaCTGGCCTTTCTGACAGATGCTTACTTACTCGCGGAACAGCGCTTCGATATTCAGCCCCCTGCGTTTGCAGGATTTCGCGCAAACGGCGCAGGCCTTc                                                      >  2:726971/1‑100 (MQ=255)
                                           gaCTGGCCTTTCTGACAGATGCTTACTTACTCGCGGAACAGCGCTTCGATATTCAGCCCCCTGCGTTTGCAGGATTTCGCGCAAACGGCGCATGCCTTCa                                                     <  2:1608831/100‑1 (MQ=255)
                                           gaCTGGCCTTTCTGACAGATGCTTACTTACTCGCGGAACAGCGCTTCGATATTCAGCCCCCTGCGTTTGCAGGATTTCGCGCAAACGGCGCAGGCCTTCa                                                     >  1:1035523/1‑100 (MQ=255)
                                           gaCTGGCCTTTCTAACAGATGCTTACTTACTGGCGGAACAGCACTTCGATATTCAGCCCCCTGCGTTTGCAGGATTTCGCGCAAACGGCGCAGGCCTTCa                                                     >  2:2065285/1‑100 (MQ=255)
                                            aCTGGCCTTTCTGACAGATGCTTACTTACTCGCGGAACAGCGCTTCGATATTCAGCCCCCTGCGTTTGCAGGATTTCGCGCAAACGGCGCAGGCCTTCaa                                                    <  1:771491/100‑1 (MQ=255)
                                             cTGGCCTTTCTGACAGATGCTTACTTACTCGCGGAACAGCGCTTCGATATTCAGCCCCCTGCGTTTGCAGGATTTCGCGCAAACGGCGCAGGCCTTCAAc                                                   <  1:2522075/100‑1 (MQ=255)
                                             cTGGCCTTTCTGACAGATGCTTACTTACTCGCGGAACAGCGCTTCGATATTCAGCCCCCTGCGTTTGCAGGATTTCGCGCAAACGGCGCAGGCCTTCAAc                                                   >  2:312697/1‑100 (MQ=255)
                                              tGGCCTTTCTGACAGATGCTTACTTACTCGCGGAACAGCGCTTCGATATTCAGCCCCCTGCGTTTGCAGGATTTCGCGCAAACGGCGCAGGCCTTCAAcc                                                  <  2:27629/100‑1 (MQ=255)
                                                 ccTTTCTGACAGATGCTTACTTACTCGCGGAACAGCGCTTCGATATTCAGCCCCCTGCGTTTGCAGGATTTCGCGCAAACGGCGCAGGCCTTCAACCTGa                                               >  1:1444903/1‑100 (MQ=255)
                                                  cTTTCTGACAGATGCTTACTTACTCGCGGAACAGCGCTTCGATATTCAGCCCCCTGCGTTTGCAGGATTTCGCGCAAACGGCGCAGGCCTTCAACCTGaa                                              >  2:1548347/1‑100 (MQ=255)
                                                      cTGACAGATGCTTACTTACTCGCGGAACAGCGCTTCGATATTCAGCCCCCTGCGTTTGCAGGATTTCGCGCAAACGGCGCAGGCCTTCAACCTGAATCTg                                          >  2:2654373/1‑100 (MQ=255)
                                                        gACAGATGCTTACTTACTCGCGGAACAGCGCTTCGATATTCAGCCCCCTGCGTTTGCAGGATTTCGCGCAAACGGCGCAGGCCTTCAACCTGAATCTGGc                                        <  2:1956270/100‑1 (MQ=255)
                                                        gACAGATGCTTACTTACTCGCGGAACAGCGCTTCGATATTCAGCCCCCTGCGTTTGCAGGATTTCGCGCAAACGGCGCAGGCCTTCAACCTGAATCTGGc                                        >  2:873606/1‑100 (MQ=255)
                                                        gACAGATGCTTACTTACTCGCGGAACAGCGCTTCGATATTCAGCCCCCTGCGTTTGCAGGATTTCGCGCAAACGGCGCAGGCCTTCAACCTGAATCTGGc                                        <  1:1152097/100‑1 (MQ=255)
                                                         aCAGATGCTTACTTACTCGCGGAACAGCGCTTCGATATTCAGCCCCCTGCGTTTGCAGGATTTCGCGCAAACGGCGCAGGCCTTCAACCTGAATCTGGCg                                       <  2:2575662/100‑1 (MQ=255)
                                                         aCAGATGCTTACTTACTCGCGGAACAGCGCTTCGATATTCAGCCCCCTGCGTTTGCAGGATTTCGCGCAAACGGCGCAGGCCTTCAACCTGAATCTGGCg                                       <  1:239659/100‑1 (MQ=255)
                                                          cAGATGCTTACTTACTCGCGGAACAGCGCTTCGATATTCAGCCCCCTGCGTTTGCAGGATTTCGCGCAAACGGCGCAGGCCTTCAACCTGAATCTGGCGa                                      <  1:2179242/100‑1 (MQ=255)
                                                          cAGATGCTTACTTACTCGCGGAACAGCGCTTCGATATTCAGCCCCCTGCGTTTGCAGGATTTCGCGCAAACGGCGCAGGCCTTCAACCTGAATCTGGCGa                                      <  2:1276577/100‑1 (MQ=255)
                                                          cAGATGCTTACTTACTCGCGGAACAGCGCTTCGATATTCAGCCCCCTGCGTTTGCAGGATTTCGCGCAAACGGCGCAGGCCTTCAACCTGAATCTGGCGa                                      >  2:564839/1‑100 (MQ=255)
                                                          cAGATGCTTACTTACTCGCGGAACAGCGCTTCGATATTCAGCCCCCTGCGTTTGCAGGATTTCGCGCAAACGGCGCAGGCCTTCAACCTGAATCTGGCGa                                      <  1:384679/100‑1 (MQ=255)
                                                          cAGATGCTTACTTACTCGCGGAACAGCGCTTCGATATTCAGCCCCCTGCGTTTGCAGGATTTCGCGCAAACGGCGCAGGCCTTCAACCCGAATCTGGCGa                                      <  2:728900/100‑1 (MQ=255)
                                                            gATGCTTACTTACTCGCGGAACAGCGCTTCGATATTCAGCCCCCTGCGTTTGCAGGATTTCGCGCAAACGGCGCAGGCCTTCAACCTGAATCTGGCGAac                                    >  1:2498853/1‑100 (MQ=255)
                                                            gATGCTTACTTACTCGCGGAACAGCGCTTCGATATTCAGCCCCCTGCGTTTGCAGGATTTCGCGCAAACGGCGCAGGCCTTCAACCTGAATCTGGCGAac                                    <  1:1469235/100‑1 (MQ=255)
                                                               gCTTACTTACTCGCGGAACAGCGCTTCGATATTCAGCCCCCTGCGTTTGCAGGATTTCGCGCAAACGGCGCAGGCCTTCAACCTGAATCTGGCGAACACg                                 >  2:3005551/1‑100 (MQ=255)
                                                               gCTTACTTACTCGCGGAACAGCGCTTCGATATTCAGCCCCCTGCGTTTGCAGGATTTCGCGCAAACGGCGCAGGCCTTCAACCTGAATCTGGCGAACACg                                 <  1:894822/100‑1 (MQ=255)
                                                                 ttacttacTCGCGGAACAGCGCTTCGATATTCAGCCCCCTGCGTTTGCAGGATTTCGCGCAAACGGCGCAGGCCTTCAACCTGAATCTGGCGAACACGtt                               <  1:2416046/100‑1 (MQ=255)
                                                                  tacttactCGCGGAACAGCGCTTCGATATTCAGCCCCCTGCGTTTGCAGGATTTCGCGCAAACGGCGCAGGCCTTCAACCTGAATCTGGCGAACACGTTc                              <  1:2619575/100‑1 (MQ=255)
                                                                    cttactCGCGGAACAGCGCTTCGATATTCAGCCCCCTGCGTTTGCAGGATTTCGCGCAAACGGCGCAGGCCTTCAACCTGAATCTGGCGAACACGTTCAc                            >  2:2502236/1‑100 (MQ=255)
                                                                    cttactCGCGGAACAGCGCTTCGATATTCAGCCCCCTGCGTTTGCAGGATTTCGCGCAAACGGCGCAGGCCTTCAACCTGAATCTGGCGAACACGTTCAc                            >  1:681907/1‑100 (MQ=255)
                                                                     ttactCGCGGAACAGCGCTTCGATATTCAGCCCCCTGCGTTTGCAGGATTTCGCGCAAACGGCGCAGGCCTTCAACCTGAATCTGGCGAACACGTTCACg                           <  1:1492821/100‑1 (MQ=255)
                                                                     ttactCGCGGAACAGCGCTTCGATATTCAGCCCCCTGCGTTTGCAGGATTTCGCGCAAACGGCGCAGGCCTTCAACCTGAATCTGGCGAACACGTTCACg                           <  2:2498867/100‑1 (MQ=255)
                                                                      tactCGCGGAACAGCGCTTCGATATTCAGCCCCCTGCGTTTGCAGGATTTCGCGCAAACGGCGCAGGCCTTCAACCTGAATCTGGCGAACACGTTCACgg                          <  2:1250851/100‑1 (MQ=255)
                                                                      tactCGCGGAACAGCGCTTCGATATTCAGCCCCCTGCGTTTGCAGGATTTCGCGCAAACGGCGCAGGCCTTCAACCTGAATCTGGCGAACACGTTCACgg                          >  2:1397334/1‑100 (MQ=255)
                                                                       actCGCGGAACAGCGCTTCGATATTCAGCCCCCTGCGTTTGCAGGATTTCGCGCAAACGGCGCAGGCCTTCAACCTGAATCTGGCGAACACGTTCACggg                         <  1:1307078/100‑1 (MQ=255)
                                                                        ctCGCGGAACAGCGCTTCGATATTCAGCCCCCTGCGTTTGCAGGATTTCGCGCAAACGGCGCAGGCCTTCAACCTGAATCTGGCGAACACGTTCACggg                         >  1:1657526/1‑99 (MQ=255)
                                                                        ctCGCGGAACAGCGCTTCGATATTCAGCCCCCTGCGTTTGCAGGATTTCGCGCAAACGGCGCAGGCCTTCAACCTGAATCTGGCGAACACGTTCACggg                         >  2:662030/1‑99 (MQ=255)
                                                                        ctCGCGGAACAGCGCTTCGATATTCAGCCCCCTGCGTTTGCAGGATTTCGCGCAAACGGCGCAGGCCTTCAACCTGAATCTGGCGAACACGTTCACGGGt                        >  1:2040659/1‑100 (MQ=255)
                                                                        ctCGCGGAACAGCGCTTCGATATTCAGCCCCCTGCGTTTGCAGGATTTCGCGCAAACGGCGCAGGCCTTCAACCTGAATCTGGCGAACACGTTCACGGGt                        >  1:1302403/1‑100 (MQ=255)
                                                                        ctCGCGGAACAGCGCTTCGATATTCAGCCCCCTGCGTTTGCAGGATTTCGCGCAAACGGCGCAGGCCTTCAACCTGAATCTGGCGAACACGTTCACGGGt                        >  1:2530040/1‑100 (MQ=255)
                                                                         tCGCGGAACAGCGCTTCGATATTCAGCCCCCTGCGTTTGCAGGATTTCGCGCAAACGGCGCAGGCCTTCAACCTGAATCTGGCGAACACGTTCACGGGTg                       >  1:502383/1‑100 (MQ=255)
                                                                          cgcgGAACAGCGCTTCGATATTCAGCCCCCTGCGTTTGCAGGATTTCGCGCAAACGGCGCAGGCCTTCAACCTGAATCTGGCGAACACGTTCACGGGTGa                      >  2:2036247/1‑100 (MQ=255)
                                                                          cgcgGAACAGCGCTTCGATATTCAGCCCCCTGCGTTTGCAGGATTTCGCGCAAACGGCGCAGGCCTTCAACCTGAATCTGGCGAACACGTTCACGGGTGa                      >  1:471509/1‑100 (MQ=255)
                                                                          cgcgGAACAGCGCTTCGATATTCAGCCCCTGCGTTTGCAGGATTTCGCGCAAACGGCGCAGGCCTTCAACCTGAATCTGGCGAACACGTTCACGGGTGAg                     >  1:2471390/1‑100 (MQ=255)
                                                                            cgGAACAGCGCTTCGATATTCAGCCCCCTGCGTTTGCAGGATTTCGCGCAAACGGCGCAGGCCTTCAACCTGAATCTGGCGAACACGTTCACGGGTGAgg                    >  1:766217/1‑100 (MQ=255)
                                                                             ggAACAGCGCTTCGATATTCAGCCCCCTGCGTTTGCAGGATTTCGCGCAAACGGCGCAGGCCTTCAACCTGAATCTGGCGAACACGTTCACGGGTGAGGc                   >  1:597805/1‑100 (MQ=255)
                                                                             ggAACAGCGCTTCGATATTCAGCCCCCTGCGTTTGCAGGATTTCGCGCAAACGGCGCAGGCCTTCAACCTGAATCTGGCGAACACGTTCACGGGTGAGGc                   >  1:1514812/1‑100 (MQ=255)
                                                                             ggAACAGCGCTTCGATATTCAGCCCCCTGCGTTTGCAGGATTTCGCGCAAACGGCGCAGGCCTTCAACCTGAATCTGGCGAACACGTTCACGGGTGAGGc                   <  2:134898/100‑1 (MQ=255)
                                                                              gAACAGCGCTTCGATATTCAGCCCCCTGCGTTTGCAGGATTTCGCGCAAACGGCGCAGGCCTTCAACCTGAATCTGGCGAACACGTTCACGGGTGAGGcc                  <  2:1191121/100‑1 (MQ=255)
                                                                              gAACAGCGCTTCGATATTCAGCCCCCTGCGTTTGCAGGATTTCGCGCAAACGGCGCAGGCCTTCAACCTGAATCTGGCGAACACGTTCACGGGTGAGGcc                  >  1:40856/1‑100 (MQ=255)
                                                                                aCAGCGCTTCGATATTCAGCCCCCTGCGTTTGCAGGATTTCGCGCAAACGGCGCAGGCCTTCAACCTGAATCTGGCGAACACGTTCACGGGTGAGGCCaa                >  1:1301133/1‑100 (MQ=255)
                                                                                aCAGCGCTTCGATATTCAGCCCCCTGCGTTTGCAGGATTTCGCGCAAACGGCGCAGGCCTTCAACCTGAATCTGGCGAACACGTTCACGGGTGAGGCCaa                >  1:1814068/1‑100 (MQ=255)
                                                                                  aGCGCTTCGATATTCAGCCCCCTGCGTTTGCAGGATTTCGCGCAAACGGCGCAGGCCTTCAACCTGAATCTGGTGAACACGTTCACGGGTGAGGCCAAtt              <  2:1868941/100‑1 (MQ=255)
                                                                                  aGCGCTTCGATATTCAGCCCCCTGCGTTTGCAGGATTTCGCGCAAACGGCGCAGGCCTTCAACCTGAATCTGGCGAACACGTTCACGGGTGAGGCCAAtt              >  1:2600398/1‑100 (MQ=255)
                                                                                     gcTTCGATATTCAGCCCCCTGCGTTTGCAGGATTTCGCGCAAACGGCGCAGGCCTTCAACCTGAATCTGGCGAACACGTTCACGGGTGAGGCCAATTTCa           <  2:1814075/100‑1 (MQ=255)
                                                                                     gcTTCGATATTCAGCCCCCTGCGTTTGCAGGATTTCGCGCAAACGGCGCAGGCCTTCAACCTGAATCTGGCGAACACGTTCACGGGTGAGGCCAATTTCa           <  2:2060475/100‑1 (MQ=255)
                                                                                      cTTCGATATTCAGCCCCCTGCGTTTGCAGGATTTCGCGCAAACGGCGCAGGCCTTCAACCTGAATCTGGCGAACACGTTCACGGGTGAGGCCAATTTCAc          <  2:1187252/100‑1 (MQ=255)
                                                                                      cTTCGATATTCAGCCCCCTGCGTTTGCAGGATTTCGCGCAAACGGCGCAGGCCTTCAACCTGAATCTGGCGAACACGTTCACGGGTGAGGCCAATTTCAc          <  2:2054284/100‑1 (MQ=255)
                                                                                      cTTCGATATTCAGCCCCCTGCGTTTGCAGGATTTCGCGCAAACGGCGCAGGCCTTCAACCTGAATCTGGCGAACACGTTCACGGGTGAGGCCAATTTCAc          >  2:1729275/1‑100 (MQ=255)
                                                                                      cTTCGATATTCAGCCCCCTGCGTTTGCAGGATTTCGCGCAAACGGCGCAGGCCTTCAACCTGAATCTGGCGAACACGTTCACGGGTGAGGCCAATTTCAc          >  2:1323426/1‑100 (MQ=255)
                                                                                       ttCGATATTCAGCCCCCTGCGTTTGCAGGATTTCGCGCAAACGGCGCAGGCCTCCAACCTGAATCTGGCGAACACGTTCACGGGTGAGGCCAATTTCACg         <  2:1497682/100‑1 (MQ=255)
                                                                                        tCGATATTCAGCCCCCTGCGTTTGCAGGATTTCGCGCAAACGGCGCAGGCCTTCAACCTGAATCTGGCGAACACGTTCACGGGTGAGGCCAATTTCACGa        <  2:1932011/100‑1 (MQ=255)
                                                                                         cGATATTCAGCCCCCTGCGTTTACAGGATTTTGCGCAGACGGCGCAGGCCTTCAACCTGAATCTGGCGAACACGTTCACGGGTGAGGCCAATTTCACGAc       <  1:2065277/100‑1 (MQ=255)
                                                                                              ttCAGCCCCCTGCGTTTGCAGGATTTCGCGCAAACGGCGCAGGCCTTCAACCTGAATCTGGCGAACACGTTCACGGGTGAGGCCAATTTCACGACCTACa  <  1:662029/100‑1 (MQ=255)
                                                                                                   |                                                                                              
ATCGGACCTTTTATTGTGCACAGAAAAGGCCAGCCTCGCTTGAGACTGGCCTTTCTGACAGATGCTTACTTACTCGCGGAACAGCGCTTCGATATTCAGCCCCTGCGTTTGCAGGATTTCGCGCAAACGGCGCAGGCCTTCAACCTGAATCTGGCGAACACGTTCACGGGTGAGGCCAATTTCACGACCTACA  >  NC_000913/2866490‑2866682

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: