BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,379,266	(C)_7→8	coding (732/1128 nt)	zapE ←	divisome ATPase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,379,259	1	.	C	96.2%	368.0 / 6.9	104	coding (739/1128 nt)	zapE	divisome ATPase
Reads supporting (aligned to +/- strand): ref base . (2/2); new base C (52/48); total (54/50)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 124 total aligned reads displayed.

CGCACAGCGTAGTAAAAGAGACCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCT‑CGTT‑T‑‑CCC‑CCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCTGAGTGAGTGTACGC  >  NC_000913/3379160‑3379353

cGCACAGCGTAGTAAAAGAGACCGCCAGCGTCTGGTTCTCGACGCACATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCT‑CGtt‑t                                                                                                   >  1:2736950/1‑100 (MQ=255)

   acaGCGTAGTAAAAGAGACCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCT‑CGTT‑T‑‑ccc                                                                                              <  1:2611793/100‑1 (MQ=255)

      gCGTAGTAAAAGAGACCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCT‑CGTT‑T‑‑ccc‑ccc                                                                                          >  1:456435/1‑100 (MQ=255)

      gCGTAGTAAAAGAGACCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCT‑CGTT‑T‑‑ccc‑ccc                                                                                          <  2:143822/100‑1 (MQ=255)

      gCGTAGTAAAAGAGACAGCCAGCGTCTAGTCCTCGACGCCCATTATTGCTAATGGCCGATGGTTGATTTCTAACGTCGCTGAATTTTCT‑CGTT‑T‑‑cac‑ccc                                                                                          >  1:124638/1‑100 (MQ=255)

        ttagtaAAAGAGACCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCT‑CGTT‑T‑‑ccc‑ccccc                                                                                        <  2:1632641/99‑2 (MQ=255)

         tagtaAAAGAGACCGCCAGCGTCTGGTTCTCGACGCCGATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCT‑CGTT‑T‑‑ccc‑ccccca                                                                                       >  1:1634723/1‑98 (MQ=255)

         tagtaAAAGAGACCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCT‑CGTT‑T‑‑ccc‑cccccg                                                                                       >  2:1447623/1‑98 (MQ=255)

         tagtaAAAGAGACCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCT‑CGTT‑T‑‑ccc‑cccccg                                                                                       >  2:2534401/1‑98 (MQ=255)

         tagtaAAAGAGACCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCT‑CGTT‑T‑‑ccc‑ccccca                                                                                       >  1:1632634/1‑98 (MQ=255)

           gtaAAAGAGACCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCT‑CGTT‑T‑‑ccc‑cccccgcc                                                                                     <  2:742918/100‑5 (MQ=255)

           gtaAAAGAGACCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCT‑CGTT‑T‑‑ccc‑cccccgcc                                                                                     <  2:2285209/100‑5 (MQ=255)

           gtaAAAGAGACCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCT‑CGTT‑T‑‑ccc‑cccccgcc                                                                                     >  2:2120232/1‑96 (MQ=255)

           gtaAAAGAGACCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCT‑CGTT‑T‑‑ccc‑cccccgcc                                                                                     >  2:1714589/1‑96 (MQ=255)

            taAAAGAGACCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCT‑CGTT‑T‑‑ccc‑cccccgcca                                                                                    >  2:141707/1‑95 (MQ=255)

            taAAAGAGACCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCT‑CGTT‑T‑‑ccc‑cccccgcca                                                                                    <  1:1941925/100‑6 (MQ=255)

             aaaaGAGACCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCT‑CGTT‑TC‑CCC‑CCCCGCCAg                                                                                    >  2:97218/1‑100 (MQ=255)

             aaaaGAGACCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCT‑CGTT‑TC‑CCC‑CCCCGCCAg                                                                                    >  2:2920054/1‑100 (MQ=255)

                agagACCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCT‑CGTT‑TC‑CCC‑CCCCGCCAgcgc                                                                                 >  2:619345/1‑100 (MQ=255)

                acagcccGCCAGCGGCTGGTTCTCGACTCCCACTCTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCT‑CGTT‑TC‑CCC‑CCCCGCCAgcgc                                                                                 <  1:494179/95‑1 (MQ=255)

                   gaCCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCT‑CGTT‑TC‑CCC‑CCCCGCCAGCGCCaa                                                                              >  2:1903290/1‑100 (MQ=255)

                     ccGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTACTGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCT‑CGTT‑TC‑CCC‑CCCCGCCAGCCCCAAcc                                                                            >  1:671290/1‑100 (MQ=255)

                     ccGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCT‑CGTT‑TC‑CCC‑CCCCGCCAGCGCCAAcc                                                                            <  1:2778425/100‑1 (MQ=255)

                     ccGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCT‑CGTT‑TC‑CCC‑CCCCGCCAGCGCCAAcc                                                                            >  2:1700512/1‑100 (MQ=255)

                     ccGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCT‑CGTT‑TC‑CCC‑CCCCGCCAGCGCCAAcc                                                                            <  1:2072550/100‑1 (MQ=255)

                      cGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCT‑CGTT‑TC‑CCC‑CCCCGCCAGCGCCAACCa                                                                           >  2:2519178/1‑100 (MQ=255)

                      cGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCT‑CGTT‑TC‑CCC‑CCCCGCCAGCGCCAACCa                                                                           >  2:1347115/1‑100 (MQ=255)

                       gCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCT‑CGTT‑TC‑CCC‑CCCCGCCAGCGCCAACCAt                                                                          <  1:1382498/100‑1 (MQ=255)

                         cAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCT‑CGTT‑TC‑CCC‑CCCCGCCAGCGCCAACCATa                                                                         <  2:2560500/99‑1 (MQ=255)

                         cAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCT‑CGTT‑TC‑CCC‑CCCCGCCAGCGCCAACCATAg                                                                        >  1:2179385/1‑100 (MQ=255)

                         cAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCT‑CGTT‑TC‑CCC‑CCCCGCCAGCGCCAACCATAg                                                                        <  2:771979/100‑1 (MQ=255)

                          aGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCT‑CGTT‑TC‑CCC‑CCCCGCCAGCGCCAACCATAGt                                                                       >  1:2184489/1‑100 (MQ=255)

                           gCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCT‑CGTT‑TC‑CCC‑CCCCGCCAGCGCCAACCATAGtt                                                                      <  1:1191976/100‑1 (MQ=255)

                             gTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGACTTTTCT‑CGTTAC‑‑CCC‑CCCCGCCAGCGCCAACAATAGTTTa                                                                    >  1:1513620/1‑100 (MQ=255)

                             gTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCT‑CGTT‑TC‑CCC‑CCCCGCCAGCGCCAACCATAGTTTa                                                                    <  2:2044820/100‑1 (MQ=255)

                             gTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGAGTTCTAACGTCGGTGAATTTTCT‑CGTT‑T‑‑CCC‑CCCCGCCAGCGCCAACCATAGTTTAt                                                                   <  1:1956920/100‑1 (MQ=255)

                               cTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCT‑CGTT‑TC‑CCC‑CCCCGCCAGCGCCAACCATAGTTTATc                                                                  <  2:456437/100‑1 (MQ=255)

                                 ggTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCT‑CGTT‑TC‑CCC‑CCCCACCAACCCCCACCATAGTTTATCCa                                                                >  1:1755677/1‑100 (MQ=255)

                                  gTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCT‑CGTT‑TC‑CCC‑CCCCGCCAGCGCCAACCATAGTTTATCCAt                                                               >  2:1020789/1‑100 (MQ=255)

                                  gTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCT‑CGTT‑TC‑CCC‑CCCCGCCAGCGCCAACCATAGTTTATCCAt                                                               >  1:2346199/1‑100 (MQ=255)

                                  gTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCT‑CGTT‑TC‑CCC‑CCCCGCCAGCGCCAACCATAGTTTATCCAt                                                               >  2:1020505/1‑100 (MQ=255)

                                   ttccgatctcccATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCT‑CGTT‑TC‑CCC‑CCCCGCCAGCGCCAACCATAGTTTATCCAtt                                                              <  2:1604262/91‑1 (MQ=255)

                                   ttCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCT‑CGTT‑TC‑CCC‑CCCCGCCAGCGCCAACCATAGTTTATCCAtt                                                              <  1:141707/100‑1 (MQ=255)

                                     ctcGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCT‑CGTT‑TC‑CCC‑CCCCGCCAGCGCCAACCATAGTTTATCCATTTg                                                            <  1:123403/100‑1 (MQ=255)

                                     ctcGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCT‑CGTT‑TC‑CCC‑CCCCGCCAGCGCCAACCATAGTTTATCCATTTg                                                            <  2:2025760/100‑1 (MQ=255)

                                     ctcGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCT‑CGTT‑TC‑CCC‑CCCCGCCAGCGCCAACCATAGTTTATCCATTTg                                                            >  2:641381/1‑100 (MQ=255)

                                     ctcGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCT‑CGTT‑TC‑CCC‑CCCCGCCAGCGCCAACCATAGTTTATCCATTTg                                                            >  2:914432/1‑100 (MQ=255)

                                       cGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCT‑CGTT‑TC‑CCC‑CCCCGCCAGCGCCAACCATAGTTTATCCATTTgcg                                                          >  1:1409279/1‑100 (MQ=255)

                                       cGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCCAACGTCGGTGAATTTTCT‑CGTT‑TC‑CCC‑CCCCGCCAGCGCCAACCATAGTTTATCCATTTgcg                                                          >  1:2098259/1‑100 (MQ=255)

                                       cGACACCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCT‑CGTT‑TC‑CCC‑CCCCGCCAGCGCCAACCATAGTTTATCCATTTgcg                                                          >  2:980274/1‑100 (MQ=255)

                                        gACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCT‑CGTT‑TC‑CCC‑CCCCGCCAGCGCCAACCATAGTTTATCCATTTgcgc                                                         >  2:2137468/1‑100 (MQ=255)

                                         aCGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCT‑CGTT‑TC‑CCC‑CCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGcc                                                        <  2:1415126/100‑1 (MQ=255)

                                            cccATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCT‑CGTT‑TC‑CCC‑CCCCGCCAGCGCCAACCCTAGTTTATCCATTTGCGCCCgg                                                     >  2:3006958/1‑100 (MQ=255)

                                            cccATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCT‑CGTT‑TC‑CCC‑CCCCGCCAGCGCCAACCATAGTTTATCCAttagatcggaa                                                     >  1:1604255/1‑91 (MQ=255)

                                            cccATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCT‑CGTT‑TC‑CCC‑CCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCgg                                                     <  1:3006939/100‑1 (MQ=255)

                                             ccATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCT‑CGTT‑TC‑CCC‑CCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCggg                                                    >  2:1285513/1‑100 (MQ=255)

                                             ccATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCT‑CGTT‑TC‑CCC‑CCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCggg                                                    >  2:1166727/1‑100 (MQ=255)

                                                     gCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCT‑CGTT‑TC‑CCC‑CCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCg                                            <  1:739112/100‑1 (MQ=255)

                                                     gCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCT‑CGTT‑TC‑CCC‑CCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCg                                            <  2:157888/100‑1 (MQ=255)

                                                     gCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCT‑CGTT‑TC‑CCC‑CCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCg                                            <  1:914429/100‑1 (MQ=255)

                                                       tAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCT‑CGTT‑TC‑CCC‑CCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTg                                          >  1:1782860/1‑100 (MQ=255)

                                                       tAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCT‑CGTT‑T‑‑CCCGCCCCGCAAGCGCCCACCATAGTTTGCCCATTTGCGCCCGGGttcaa                                               >  1:308865/1‑92 (MQ=255)

                                                         aTGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCT‑CGTT‑TC‑CCC‑CCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGaa                                        <  2:2001741/100‑1 (MQ=255)

                                                         aTGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCT‑CGTT‑TC‑CCC‑CCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGaa                                        >  1:1291512/1‑100 (MQ=255)

                                                           ggCCGATGGTTGATTTCTAACGTCGGTGAATTTTCT‑CGTT‑TC‑CCC‑CCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGt                                      >  2:221158/1‑100 (MQ=255)

                                                           ggCCGATGGTTGATTTCTAACGTCGGTGAATTTTCT‑CGTT‑TC‑CCC‑CCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGt                                      >  2:3017223/1‑100 (MQ=255)

                                                            gCCGATGGTTGATTTCTAACGTCGGTGAATTTTCT‑CGTT‑TCCCCC‑CCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGt                                      >  1:2536769/1‑100 (MQ=255)

                                                               gATGGTTGATTTCTAACGTCGGTGAATTTTCT‑CGTT‑TC‑CCC‑CCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCg                                  <  1:101904/100‑1 (MQ=255)

                                                                 tGGTTGATTTCTAACGTCGGTGAATTTTCT‑CGTT‑TCCCCC‑CCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGa                                 >  1:107258/1‑100 (MQ=255)

                                                                   gTTGATTTCCAACGTCCGTGAATTTTTTCCGTT‑CC‑CCC‑CCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGaaa                               <  1:320807/100‑1 (MQ=255)

                                                                    ttGATTTCTAACGTCGGTGAATTTTCT‑CGTT‑TC‑CCC‑CCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGc                             >  2:326380/1‑100 (MQ=255)

                                                                     tGATTTCTAACGTCGGTGAATTTTCT‑CGTT‑TC‑CCC‑CCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGc                             >  1:92268/1‑99 (MQ=255)

                                                                       aTTTCTAACGTCGGTGAATTTTCT‑CGTT‑TC‑CCC‑CCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCcac                          >  1:2971886/1‑100 (MQ=255)

                                                                       aTTTCTAACGTCGGTGAATTTTCT‑CGTT‑TC‑CCC‑CCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCcac                          >  2:2152141/1‑100 (MQ=255)

                                                                        tttCTAACGTCGGTGAATTTTCT‑CGTT‑TC‑CCC‑CCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCcaca                         <  2:727684/100‑1 (MQ=255)

                                                                        tttCTAACGTCGGTGAATTTTCT‑CGTT‑TC‑CCC‑CCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCcaca                         <  1:619344/100‑1 (MQ=255)

                                                                             aaCGTCGGTGAATTTTCT‑CGTT‑TC‑CCC‑CCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATgc                    >  1:459321/1‑100 (MQ=255)

                                                                             aaCGTCGGTGAATTTTCT‑CGTT‑TC‑CCC‑CCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATgc                    >  2:2861109/1‑100 (MQ=255)

                                                                              aCGTCGGTGAATTTTCT‑CGTT‑TC‑CCC‑CCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATgcg                   >  2:914074/1‑100 (MQ=255)

                                                                                cgatctGAATTTTCT‑CGTT‑TC‑CCC‑CCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATgcgcn                 <  1:420458/95‑2 (MQ=255)

                                                                                  cGGTGAATTTTCT‑CGTT‑TC‑CCC‑CCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCtg               <  1:688820/100‑1 (MQ=255)

                                                                                  cGGTGAATTTTCT‑CGTT‑TC‑CCC‑CCCCGCCAGCGCCAACCATAGTGTATCCATTTGCGCCCGGGTTTCATCGTGAAATGGCGAAAGCCACAAATTCGcc                 >  1:2247465/1‑98 (MQ=255)

                                                                                    gTGAATTTTCT‑CGTT‑TC‑CCC‑CCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCtgag             <  1:1903283/100‑1 (MQ=255)

                                                                                     tGAATTTTCT‑CGTT‑TC‑CCC‑CCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCtgagt            <  1:2519164/100‑1 (MQ=255)

                                                                                      gAATTTTCT‑CGTT‑TC‑CCC‑CCCCGCCAGCGCCAACCATAGTTTACCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCtgagtg           <  1:1954187/100‑1 (MQ=255)

                                                                                        aTTTTCT‑CGTT‑TC‑CCC‑CCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCtgagtgag         <  1:1941144/100‑1 (MQ=255)

                                                                                        aTTTTCT‑CGTT‑TC‑CCC‑CCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCtgagtgag         >  1:3047726/1‑100 (MQ=255)

                                                                                        aTTTTCT‑CGTT‑TC‑CCC‑CCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCtgagtgag         <  1:1714582/100‑1 (MQ=255)

                                                                                         ttttCT‑CGTTCC‑‑CCC‑CCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCTgagtgagt        <  2:1755684/100‑1 (MQ=255)

                                                                                         ttttCT‑CGTT‑TCCCCC‑CCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCtgagtgag         <  2:107258/100‑1 (MQ=255)

                                                                                          tttCT‑CGTT‑TC‑CCC‑CCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCTGagtgagtg       <  2:2743756/100‑1 (MQ=255)

                                                                                          tttCT‑CGTT‑TC‑CCC‑CCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCTGagtgagtg       >  2:794290/1‑100 (MQ=255)

                                                                                          tttCT‑CGTT‑TC‑CCC‑CCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCTGagtgagtg       <  2:198171/100‑1 (MQ=255)

                                                                                           ttCT‑CGTT‑TC‑CCC‑CCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCTGAGTGAgtgt      >  2:1533614/1‑100 (MQ=255)

                                                                                           ttCT‑CGTT‑TC‑CCC‑CCCCGCCAGCGCCAACCATAGTTTATACATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCTGAGTGAgtgt      >  1:2825954/1‑100 (MQ=255)

                                                                                             ct‑cGTT‑TC‑CCC‑CCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCTGAGTGAGTGTAc    <  2:2736966/100‑1 (MQ=255)

                                                                                             ct‑cGTT‑TC‑CCC‑CCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCTGAGTGAGTGTAc    >  2:2144308/1‑100 (MQ=255)

                                                                                              t‑cGTT‑TC‑CCC‑CCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCTGAGTGAGTGTACg   <  2:2998913/100‑1 (MQ=255)

                                                                                              t‑cGTT‑TC‑CCC‑CCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCTGAGTGAGTGTACg   <  2:1261201/100‑1 (MQ=255)

                                                                                                cGTT‑TC‑CCC‑CCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCTGAGTGAGTGTACGc  >  2:470479/1‑100 (MQ=255)

CGCACAGCGTAGTAAAAGAGACCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCT‑CGTT‑T‑‑CCC‑CCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCTGAGTGAGTGTACGC  >  NC_000913/3379160‑3379353

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`