Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,246,073	C→T	G435D (GGC→GAC)	treA ←	periplasmic trehalase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,246,073	0	C	T	100.0%	313.8 / NA	88	G435D (GGC→GAC)	treA	periplasmic trehalase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (44/44);  total (44/44)

GTTTTGTAATCCTTCTGTCGCGACCCACTGTAACGGTGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGCCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCATTTTGTTGGCGCGATCTTTCGCTGCCGCATTGACGTACAGCGGGAACAGGGCGGCC  >  NC_000913/1245979‑1246168                                                                                               |                                                                                                gTTTTGTAATCCTTCTGTCGCGACCCACTGTAACGGTGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGtcgccg                                                                                            >  2:36015/1‑100 (MQ=255) gTTTTGTAATCCTTCTGTCGCGACCCACTGTAACGGTGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGATGTTCAGGtcgccg                                                                                            >  1:2087153/1‑100 (MQ=255)   tttGTAATCCTTCTGTCGCGACCCACTGTAACGGTGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGTCGCCggg                                                                                          <  1:2589478/100‑1 (MQ=255)   tttGTAATCCTTCTGTCGCGACCCACTGTAACGGTGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGTCGCCggg                                                                                          <  1:1944241/100‑1 (MQ=255)   tttGTAATCCTTCTGTCGCGACCCACTGTAACGGTGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGTCGCCggg                                                                                          >  2:546835/1‑100 (MQ=255)   tcttccgatcTTCTGTCGCGACCCACTGTAACGGTGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGTCGCCggg                                                                                          <  2:2679191/91‑1 (MQ=255)    ttGTAATCCTTCTGTCGCGACCCACTGTAACGGTGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCCCCGACGTGGTGTTCAGGTCGCCggg                                                                                          >  2:1500367/1‑99 (MQ=255)    ttGTAATCCTTCTGTCGCGACCCACTGTAACGGTGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGTCGCCGGGt                                                                                         <  1:1314598/100‑1 (MQ=255)    ttGTAATCCTTCTGTCGCGACCCACTGTAACGGTGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGTCGCCGGGt                                                                                         <  2:2583413/100‑1 (MQ=255)        aaTCCTTCTGTCGCGACCCACTGTAACGGTGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGTCGCCGGGTTgca                                                                                     <  2:1273257/100‑1 (MQ=255)         aTCCTTCTGTCGCGACCCACTGTAACGGTGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGTCGCCGGGTTgcag                                                                                    <  2:685161/100‑1 (MQ=255)          tCCTTCTGTCGCGACCCACTGTAACGGTGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGTCGCCGGGTTgcagc                                                                                   <  2:1098093/100‑1 (MQ=255)          tCCTTCTGTCGCGACCCACTGTAACGGTGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGTCGCCGGGTTgcagc                                                                                   <  2:984454/100‑1 (MQ=255)           ccTTCTGTCGCGACCCACTGTAACGGTGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGTCGCCGGGTTgcagca                                                                                  <  1:3053216/100‑1 (MQ=255)            cTTCTGTCGCGACCCACTGTAACGGTGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGTCGCCGGGTTGcagcag                                                                                 <  1:740583/100‑1 (MQ=255)               cTGTCGCGACCCACTGTAACGGTGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGTCGCCGGGTTGCAGCAGAtg                                                                              <  2:1592771/100‑1 (MQ=255)                 gTCGCGACCCACTGTAACGGTGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGTCGCCGGGTTGCAGCAGAtgtg                                                                            >  2:1067170/1‑100 (MQ=255)                 gTCGCGACCCACTGTAACGGTGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGTCGCCGGGTTGCAGCAGAtgtg                                                                            <  1:3031543/100‑1 (MQ=255)                 gTCGCGACCCACTGTAACGGTGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGTCGCCGGGTTGCAGCAGAtgtg                                                                            <  1:198781/100‑1 (MQ=255)                 gTCGCGACCCACTGTAACGGTGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGTCGCCGGGTTGCAGCAGAtgtg                                                                            <  2:813799/100‑1 (MQ=255)                  tCGCGACCCACTGTAACGGTGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGTCGCCGGGTTGCAGCAGATgtgt                                                                           >  2:185319/1‑100 (MQ=255)                   cgcgACCCACTGTAACGGTGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGTCGCCGGGTTGCAGCAGATGTGtt                                                                          >  2:1131937/1‑100 (MQ=255)                    gcgACCCACTGTAACGGTGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGTCGCCGGGTTGCAGCAGATGTGttt                                                                         >  2:618317/1‑100 (MQ=255)                         ccACTGTAACGGTGCCCAGCCATTTGGCGCATGCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGTCGCCGGGTTGCAGCAGATGTGTTTtcgtc                                                                    <  2:890169/100‑1 (MQ=255)                         ccACTGTAACGGTGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGTCGCCGGGTTGCAGCAGATGTGTTTtcgtc                                                                    >  2:303021/1‑100 (MQ=255)                          cACTGTAACGGTGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGTCGCCGGGTTGCAGCAGATGTGTTTtcgtcg                                                                   >  1:1041960/1‑100 (MQ=255)                           aCTGTAACGGTGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGTCGCCGGGTTGCAGCAGATGTGTTTTcgtcgc                                                                  <  1:1917562/100‑1 (MQ=255)                             tGTAACGGTGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGTCGCCGGGTTGCAGCAGATGTGTTTTcgtcgccg                                                                <  2:712571/100‑1 (MQ=255)                             tGTAACGGTGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGTCGCCGGGTTGCAGCAGATGTGTTTTcgtcgccg                                                                >  2:1097845/1‑100 (MQ=255)                              gTAACGGTGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGTCGCCGGGTTGCAGCAGATGTGTTTTcgtcgccgt                                                               >  2:744492/1‑100 (MQ=255)                              gTAACGGTGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGTCGCCGGGTTGCAGCAGATGTGTTTTcgtcgccga                                                               >  1:712570/1‑99 (MQ=255)                              gTAACGGTGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGTCGCCGGGTTGCAGCAGATGTGTTTTcgtcgccg                                                                >  1:755105/1‑99 (MQ=255)                                aaCGGTGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGTCGCCGGGTTGCAGCAGATGTGTTTTcgtcgccgtcg                                                             >  1:2261355/1‑100 (MQ=255)                                     tGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGTCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCAtt                                                        >  2:2279790/1‑100 (MQ=255)                                      gCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTGCACCGACGTGGTGTTCAGGTCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTAGCCAttt                                                       >  1:1648661/1‑100 (MQ=255)                                       cccAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGTCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCAtttt                                                      >  2:1290516/1‑100 (MQ=255)                                        ccAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGTCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCATTttg                                                     <  2:1386675/100‑1 (MQ=255)                                        ccAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGTCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCATTttg                                                     >  2:214958/1‑100 (MQ=255)                                         cAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGTCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCATTttgt                                                    >  2:3065326/1‑100 (MQ=255)                                         cAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGTCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCATTttgt                                                    >  1:276220/1‑100 (MQ=255)                                          aGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGTCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCATTttgtt                                                   <  2:755107/100‑1 (MQ=255)                                            ccATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGTCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCATTTTGTTgg                                                 <  2:1930019/100‑1 (MQ=255)                                            ccATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGTCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCATTTTGTTgg                                                 <  1:2617064/100‑1 (MQ=255)                                              aTTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGTCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCATTTTGTTGgcg                                               <  2:549767/100‑1 (MQ=255)                                              aTTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGTCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCATTTTGTTGgcg                                               >  2:1633467/1‑100 (MQ=255)                                               tttGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGTCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCATTTTGTTGgcgc                                              <  1:1290512/100‑1 (MQ=255)                                                 tGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGTCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCATTTTGTTGGCGCGa                                            >  2:935367/1‑100 (MQ=255)                                                  ggCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGTCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCATTTTGTTGGCGCGAt                                           >  1:1104391/1‑100 (MQ=255)                                                    cgcATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGTCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCATTTTGTTGGCGCGATCt                                         <  1:280855/100‑1 (MQ=255)                                                      cATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGTCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCATTTTGTTGGCGCGATCttt                                       <  1:1097841/100‑1 (MQ=255)                                                       aTCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGTCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCATTTTGTTGGCGCGATCTTTc                                      <  1:185319/100‑1 (MQ=255)                                                        tCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGTCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCATTTTGTTGGCGCGATCTTTCg                                     >  1:1498377/1‑100 (MQ=255)                                                         cccATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGTCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCATTTTGTTGGCGCGATCTTTCGc                                    >  2:2662762/1‑100 (MQ=255)                                                         cccATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGTCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCATTTTGTTGGCGCGATCTTTCGc                                    >  1:753101/1‑100 (MQ=255)                                                         cccATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGTCGCCGGGATGCAGCAGATGTGTTTTCGTCGCCGTCGCCATTTTGTTGGCGCGATCTTTCGc                                    >  1:1697824/1‑100 (MQ=255)                                                          ccATTGTTGCCCACTTTTCACCGTCGTGTTGTTCAGGTCGCCGGGTTGCAGCACATGTGTTTTCGTCGCCGTCGCCACTTTGTTGGCGCGATCTTTCGc                                    <  1:732536/99‑1 (MQ=255)                                                           cATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGTCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCATTTTGTTGGCGCGATCTTTCGCTg                                  >  1:385544/1‑100 (MQ=255)                                                           cATTGTTGCCCACTTTTCACCGACGTGGTGATCAGGTCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCATTTTGTTGGCGCGATCTTTCGc                                    >  1:939051/1‑98 (MQ=255)                                                            aTTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGTCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCATTTTGTTGGCGCGATCTTTCGCTGc                                 <  1:214958/100‑1 (MQ=255)                                                             ttgttgCCCACTTTTCACCGACGTGGTGTTCAGGTCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCATTTTGTTGGCGCGATCTTTCGCTGcc                                <  1:1551283/100‑1 (MQ=255)                                                              tgttgCCCACTTTTCACCGACGTGGTGGTCAGGTCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCATTTTGTTGGCGCGATCTTTCGCTGCCg                               <  2:879140/100‑1 (MQ=255)                                                               gttgCCCACTTTTCACCGACGTGGTGTTCAGGTCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCATTTTGTTGGCGCGATCTTTCGCTGCCGc                              >  2:1575247/1‑100 (MQ=255)                                                                ttgCCCACTTTTCACCGACGTGGTGTTCAGGTCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCATTTTGTTGGCGCGATCTTTCGCTGCCGCa                             <  1:1067166/100‑1 (MQ=255)                                                                  gCCCACTTTTCACCGACGTGGTGTTCAGGTCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCATTTTGTTGGCGCGATCTTTCGCTGCCGCAtt                           >  2:2502278/1‑100 (MQ=255)                                                                   cccACTTTTCACCGACGTGGTGTTCAGGTCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCATTTTGTTGGCGCGATCTTTCGCTGCCGCATTg                          >  1:2466313/1‑100 (MQ=255)                                                                   cccACTTTTCACCGACGTGGTGTTCAGGTCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCATTTTGTTGGCGCGATCTTTCGCTGCCGCATTg                          <  2:206936/100‑1 (MQ=255)                                                                    ccACTTTTCACCGACGTGGTGTTCAGGTCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCATTTTGTTGGCGCGATCTTTCGCTGCCGCATTGa                         >  2:1487470/1‑100 (MQ=255)                                                                      aCTTTTCACCGACGTGGTGTTCAGGTCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCATTTTGTTGGCGCGATCTTTCGCTGCCGCATTGACg                       <  2:2034730/100‑1 (MQ=255)                                                                      aCTTTTCACCGACGTGGTGTTCAGGTCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCATTTTGTTGGCGCGATCTTTCGCTGCCGCATTGACg                       <  2:2034679/100‑1 (MQ=255)                                                                        ttttCACCGACGTGGTGTTCAGGTCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCATTTTGTTGGCGCGATCTTTCGCTGCCGCATTGACGTa                     <  1:730884/100‑1 (MQ=255)                                                                         tttCACCGACGTGGTGTTCAGGTCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCATTTTGTTGGCGCGATCTTTCGCTGCCGCATTGACGTAc                    <  2:882495/100‑1 (MQ=255)                                                                            cACCGACGTGGTGTTCAGGTCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCATTTTGTTGGCGCGATCTTTCGCTGCCGCATTGACGTACAGc                 <  1:1462401/100‑1 (MQ=255)                                                                            cACCGACGTGGTGTTCAGGTCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCATTTTGTTGGCGCGATCTTTCGCTGCCGCATTGACGTACAGc                 >  2:454987/1‑100 (MQ=255)                                                                                gACGTGGTGTTCAGGTCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCATTTTGTTGGCGCGATCTTTCGCTGCCGCATTGACGTACAGCGGGa             >  2:132553/1‑100 (MQ=255)                                                                                  cGTGGTGTTCAGGTCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCATTTTGTTGGCGCGATCTTTCGCTGCCGCATTGACGTACAGCGGGAAc           <  1:303020/100‑1 (MQ=255)                                                                                   gtggtgTTCAGGTCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCATTTTGTTGGCGCGATCTTTCGCTGCCGCATTGACGTACAGCGGGAACa          >  1:1637881/1‑100 (MQ=255)                                                                                   gtggtgTTCAGGTCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCATTTTGTTGGCGCGATCTTTCGCTGCCGCATTGACGTACAGCGGGAACa          <  1:1575675/100‑1 (MQ=255)                                                                                    tggtgTTCAGGTCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCATTTTGTTGGCGCGATCTTTCGCTGCCGCATTGACGTACAGCGGGAACAg         >  2:2410039/1‑100 (MQ=255)                                                                                    tggtgTTCAGGTCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCATTTTGTTGGCGCGATCTTTCGCTGCCGCATTGACGTACAGCGGGAACAg         >  2:2835197/1‑100 (MQ=255)                                                                                    tggtgTTCAGGTCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCATTTTGTTGGCGCGATCTTTCGCTGCCGCATTGACGTACAGCGGGAACAg         <  1:1521094/100‑1 (MQ=255)                                                                                     ggtgTTCAGGTCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCATTTTGTTGGCGCGATCTTTCGCTGCCGCATTGACGTACAGCGGGAACAgg        >  1:273861/1‑100 (MQ=255)                                                                                      gtgtTCAGGTCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCATTTTGTTGGCGCGATCTTTCGCTGCCGCATTGACGTACAGCGGGAACAggg       <  2:1648668/100‑1 (MQ=255)                                                                                       tgtTCAGGTCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCATTTTGTTGGCGCGATCTTTCGCTGCCGCATTGACGTACAGCGGGAACAGggc      <  2:1065150/100‑1 (MQ=255)                                                                                       tgtTCAGGTCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCATTTTGTTGGCGCGATCTTTCGCTGCCGCATTGACGTACAGCGGGAACAGggc      >  2:1653871/1‑100 (MQ=255)                                                                                       tgtTCAGGTCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCATTTTGTTGGCGCGATCTTTCGCTGCCGCATTGACGTACAGCGGGAACAGggc      <  2:1742484/100‑1 (MQ=255)                                                                                        gtTCAGGTCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCATTTTGTTGGCGCGATCTTTCGCTGCCGCATTGACGTACAGCGGGAACAGggcg     >  2:1855866/1‑100 (MQ=255)                                                                                         ttCAGGTCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCATTTTGTTGGCGCGATCTTTCGCTGCCGCATTGACGTACAGCGGGAACAGggcgg    >  1:1152135/1‑100 (MQ=255)                                                                                          tCAGGTCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCATTTTGTTGGCGCGATCTTTCGCTGCCGCATTGACGTACAGCGGGAACAGggcggc   >  1:819380/1‑100 (MQ=255)                                                                                          tCAGGTCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCATTTTGTTGGCGCGATCTTTCGCTGCCGCATTGACGTACAGCGGGAACAGggcggc   >  2:2024422/1‑100 (MQ=255)                                                                                           cAGGTCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCATTTTGTTGGCGCGATCTTTCGCTGCCGCATTGACGTACAGCGGGAACAGGGCGGcc  >  1:1191682/1‑100 (MQ=255)                                                                                               |                                                                                                GTTTTGTAATCCTTCTGTCGCGACCCACTGTAACGGTGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGCCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCATTTTGTTGGCGCGATCTTTCGCTGCCGCATTGACGTACAGCGGGAACAGGGCGGCC  >  NC_000913/1245979‑1246168

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑