BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,059,919	C→T	Q505* (CAG→TAG)	typA →	GTP‑binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,059,919	0	C	T	97.6%	419.0 / 0.1	123	Q505* (CAG→TAG)	typA	GTP‑binding protein
Reads supporting (aligned to +/- strand): ref base C (1/2); new base T (54/66); total (55/68)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.94e-01

Only 100 of 123 total aligned reads displayed.

CGACGACGTACGTCCGGGTGAAGTGGGTCAGCGTCAGAACGGCGTACTGATCTCTAACGGTCAGGGTAAAGCGGTCGCGTTCGCGCTGTTCGGTCTGCAGGATCGCGGTAAGCTGTTCCTCGGTCACGGTGCAGAAGTTTACGAAGGTCAGATTATCGGTATTCATAGCCGCTCTAACGACCTGACTGTAAAC  >  NC_000913/4059822‑4060014

cgacgacgTACGTCCGGGTGAAGTGGGTCAGCGTCAGAACGGCGTACTGATCTCTAACGGTCAGGGTAAAGCGGTCGCGTTCGCGCTGTTCGGTCTGTAg                                                                                               <  2:860634/100‑1 (MQ=255)

 gacgacgTACGTCCGGGTGAAGTGGGTCAGCGTCAGAACGGCGTACTGATCTCTAACGGTCAGGGTAAAGCGGTCGCGTTCGCGCTGTTCGGTCTGTAgg                                                                                              <  1:468024/100‑1 (MQ=255)

  acgacgTACGTCCGGGTGAAGTGGGTCAGCGTCAGAACGGCGTACTGATCTCTAACGGTCAGGGTAAAGCGGTCGCGTTCGCGCTGTTCGGTCTGTAGGa                                                                                             <  2:1825748/100‑1 (MQ=255)

   cgacgTACGTCCGGGTGAAGTGGGTCAGCGTCAGAACGGCGTACTGATCTCTAACGGTCAGGGTAAAGCGGTCGCGTTCGCGCTGTTCGGTCTGTAGGAt                                                                                            <  2:763259/100‑1 (MQ=255)

      cgtacgtCCGGGTGAAGTGGGTCAGCGTCAGAACGGCGTACTGATCTCTAACGGTCAGGGTAAAGCGGTCGCGTTCGCGCTGTTCGGTCTGTAGGATcgc                                                                                         <  2:1439901/100‑1 (MQ=255)

        tacgtCCGGGTGAAGTGGGTCAGCGTCAGAACGGCGTACTGATCTCTAACGGTCAGGGTAAAGCGGTCGCGTTCGCGCTGGTCGGTCTGTAGGATcgcg                                                                                        >  2:1213290/1‑99 (MQ=255)

            tCCGGGTGAAGTGGGTCAGCGTCAGAACGGCGTACTGATCTCTAACGGTCAGGGTAAAGCGGTCGCGTTCGCGCTGTTCGGTCTGTAGGATCGCGGTAAg                                                                                   <  2:598328/100‑1 (MQ=255)

            tCCGGGTGAAGTGGGTCAGCGTCAGAACGGCGTACTGATCTCTAACGGTCAGGGTAAAGCGGTCGCGTTCGCGCTGTTCGGTCTGTAGGATCGCGGTAAg                                                                                   >  1:1485273/1‑100 (MQ=255)

             ccGGGTGAAGTGGGTCAGCGTCAGAACGGCGTACTGATCTCTAACGGTCAGGGTAAAGCGGTCGCGTTCGCGCTGTTCGGTCTGTAGGATCGCGGTAAGc                                                                                  <  1:2686840/100‑1 (MQ=255)

             ccGGGTGAAGTGGGTCAGCGTCAGAACGGCGTACTGATCTCTAACGGTCAGGGTAAAGCGGTCGCGTTCGCGCTGTTCGGTCTGTAGGATCGCGGTAAGc                                                                                  <  2:1111679/100‑1 (MQ=255)

             ccGGGTGAAGTGGGTCAGCGTCAGAACGGCGTACTGATCTCTAACGGTCAGGGTAAAGCGGTCGCGTTCGCGCTGTTCGGTCTGTAGGATCGCGGTAAGc                                                                                  <  2:1652670/100‑1 (MQ=255)

              cGGGTGAAGTGGGTCAGCGTCAGAACGGCGTACTGATCTCTAACGGTCAGGGTAAAGCGGTCGCGTTCGCGCTGTTCGGTCTGTAGGATCGCGGTAAGCt                                                                                 <  1:2791400/100‑1 (MQ=255)

               gggTGAAGTGGGTCAGCGTCAGAACGGCGTACTGATCTCTAACGGTCAGGGTAAAGCGGTCGCGTTCGCGCTGTTCGGTCTGTAGGATCGCGGTAAGCTg                                                                                >  1:161567/1‑100 (MQ=255)

                  tGAAGTGGGTCAGCGTCAGAACGGCGTACTGATCTCTAACGGTCAGGGTAAAGCGGTCGCGTTCGCGCTGTTCGGTCTGTAGGATCGCGGTAAGCTGTTc                                                                             <  2:154466/100‑1 (MQ=255)

                  tGAAGTGGGTCAGCGTCAGAACGGCGTACTGATCTCTAACGGTCAGGGTAAAGCGGTCGCGTTCGCGCTGTTCGGTCTGTAGGATCGCGGTAAGCTGTTc                                                                             >  1:1154892/1‑100 (MQ=255)

                   gAAGTGGGTCAGCGTCAGAACGGCGTACTGATCTCTAACGGTCAGGGTAAAGCGGTCGCGTTCGCGCTGTTCGGTCTGTAGGATCGCGGTAAGCTGTTcc                                                                            <  1:761007/100‑1 (MQ=255)

                   gAAGTGGGTCAGCGTCAGAACGGCGTACTGATCTCTAACGGTCAGGGTAAAGCGGTCGCGTTCGCGCTGTTCGGTCTGTAGGATCGCGGTAAGCTGTTcc                                                                            <  2:604149/100‑1 (MQ=255)

                   gAAGTGGGTCAGCGTCAGAACGGCGTACTGATCTCTAACGGTCAGGGTAAAGCGGTCGCGTTCGCGCTGTTCGGTCTGCAGGATCGCGGTAAGCTGTTcc                                                                            <  1:959498/100‑1 (MQ=255)

                    aaGTGGGTCAGCGTCAGAACGGCGTACTGATCTCTAACGGTCAGGGTAAAGCGGTCGCGTTCGCGCTGTTCGGTCTGTAGGATCGCGGTAAGCTGTTCCt                                                                           <  1:1023587/100‑1 (MQ=255)

                      gTGGGTCAGCGTCAGAACGGCGTACTGATCTCTAACGGTCAGGGTAAAGCGGTCGCGTTCGCGCTGTTCGGTCTGTAGGATCGCGGTAAGCTGTTCCTCg                                                                         <  1:1105653/100‑1 (MQ=255)

                      gTGGGTCAGCGTCAGAACGGCGTACTGATCTCTAACGGTCAGGGTAAAGCGGTCGCGTTCGCGCTGTTCGGTCTGTAGGATCGCGGTAAGCTGTTCCTCg                                                                         >  1:877154/1‑100 (MQ=255)

                       tGGGTCAGCGTCAGAACGGCGTACTGATCTCTAACGGTCAGGGTAAAGCGGTCGCGTTCGCGCTGTTCGGTCTGTAGGATCGCGGTAAGCTGTTCCTCgg                                                                        <  2:688602/100‑1 (MQ=255)

                       tGGGTCAGCGTCAGAACGGCGTACTGATCTCTAACGGTCAGGGTAAAGCGGTCGCGTTCGCGCTGTTCGGTCTGTAGGATCGCGGTAAGCTGTTCCTCgg                                                                        <  2:916011/100‑1 (MQ=255)

                       tGGGTCAGCGTCAGAACGGCGTACTGATCTCTAACGGTCAGGGTAAAGCGGTCGCGTTCGCGCTGTTCGGTCTGTAGGATCGCGGTAAGCTGTTCCTCgg                                                                        <  2:2242372/100‑1 (MQ=255)

                       tGGGTCAGCGTCAGAACGGCGTACTGATCTCTAACGGTCAGGGTAAAGCGGTCGCGTTCGCGCTGTTCGGTCTGTAGGATCGCGGTAAGCTGTTCCTCgg                                                                        >  2:1781790/1‑100 (MQ=255)

                       tGGGTCAGCGTCAGAACGGCGTACTGATCTCTAACGGTCAGGGTAAAGCGGTCGCGTTCGCGCTGTTCGGTCTGTAGGATCGCGGTAAGCTGTTCCTCgg                                                                        <  2:1490185/100‑1 (MQ=255)

                        gggTCAGCGTCAGAACGGCGTACTGATCTCTAACGGTCAGGGTAAAGCGGTCGCGTTCGCGCTGTTCGGTCTGTAGGATCGCGGTAAGCTGTTCCTCGGt                                                                       <  2:1213745/100‑1 (MQ=255)

                        atctCAGCGTCAGAACGGCGTACTGATCTCTAACGGTCAGGGTAAAGCGGTCGCGTTCGCGCTGTTCGGTCTGTAGGATCGCGGTAAGCTGTTCCTCGGt                                                                       <  1:2414793/97‑1 (MQ=255)

                         ggTCAGCGTCAGAACGGCGTACTGATCTCTAACGGTCAGGGTAAAGCGGTCGCGTTCGCGCTGTTCGGTCTGTAGGATCGCGGTAAGCTGTTCCTCGGTc                                                                      >  2:428446/1‑100 (MQ=255)

                         ggTCAGCGTCAGAACGGCGTACTGATCTCTAACGGTCAGGGTAAAGCGGTCGCGTTCGCGCTGTTCGGACTGTAGGATCGCGGTAAGCTGTTCCTCGGTc                                                                      >  1:192174/1‑100 (MQ=255)

                          gTCAGCGTCAGAACGGCGTACTGATCTCTAACGGTCAGGGTAAAGCGGTCGCGTTCGCGCTGTTCGGTCTGTAGGATCGCGGTAAGCTGTTCCTCGGTCa                                                                     >  1:173407/1‑100 (MQ=255)

                          gTCAGCGTCAGAACGGCGTACTGATCTCTAACGGTCAGGGTAAAGCGGTCGCGTTCGCGCTGTTCGGTCTGTAGGATCGCGGTAAGCTGTTCCTCGGTCa                                                                     >  1:267419/1‑100 (MQ=255)

                           tCAGCGTCAGAACGGCGTACTGATCTCTAACGGTCAGGGTAAAGCGGTCGCGTTCGCGCTGTTCGGTCTGTAGGATCGCGGTAAGCTGTTCCTCGGTCAc                                                                    <  1:1457491/100‑1 (MQ=255)

                            cAGCGTCAGAACGGCGTACTGATCTCTAACGGTCAGGGTAAAGCGGTCGCGTTCGCGCTGTTCGGTCTGTAGGATCGCGGTAAGCTGTTCCTCGGtagat                                                                   >  2:2414804/1‑96 (MQ=255)

                               cGTCAGAACGGCGTACTGATCTCTAACGGTCAGGGTAAAGCGGTCGCGTTCGCGCTGTTCGGTCTGTAGGATCGCGGTAAGCTGTTCCTCGGTCACGGTg                                                                <  1:2248329/100‑1 (MQ=255)

                                  cAGAACGGCGTACTGATCTCTAACGGTCAGGGTAAAGCGGTCGCGTTCGCGCTGTTCGGTCTGTAGGATCGCGGTAAGCTGTTCCTCGGTCACGGTGCAg                                                             <  1:133285/100‑1 (MQ=255)

                                   aGAACGGCGTACTGATCTCTAACGGTCAGGGTAAAGCGGTCGCGTTCGCGCTGTTCGGTCTGTAGGATCGCGGTAAGCTGTTCCTCGGTCACGGTGCAGa                                                            >  1:254899/1‑100 (MQ=255)

                                   aGAACGGCGTACTGATCTCT‑ACGGTCAGGGTAAAGCGGTCGCGTTCGCGCTGTTCGGTCTGTAGGATCGCGGTAAGCTGTTCCTCGGTCACGGTGCAGaa                                                           >  1:254977/1‑100 (MQ=255)

                                    gAACGGCGTACTGATCTCTAACGGTCAGGGTAAAGCGGTCGCGTTCGCGCTGTTCGGTCTGTAGGATCGCGGTAAGCTGTTCCTCGGTCACGGTGCAGaa                                                           >  1:1103369/1‑100 (MQ=255)

                                     aaCGGCGTACTGATCTCTAACGGTCAGGGTAAAGCGGTCGCGTTCGCGCTGTTCGGTCTGTAGGATCGCGGTAAGCTGTTCCTCGGTCACGGTGCAGAAg                                                          <  2:351867/100‑1 (MQ=255)

                                     aaCGGCGTACTGATCTCTAACGGTCAGGGTAAAGCGGTCGCGTTCGCGCTGTTCGGTCTGTAGGATCGCGGTAAGCTGTTCCTCGGTCACGGTGCAGAAg                                                          <  1:613635/100‑1 (MQ=255)

                                      aCGGCGTACTGATCTCTAACGGTCAGGGTAAAGCGGTCGCGTTCGCGCTGTTCGGTCTGTAGGATCGCGGTAAGCTGTTCCTCGGTCACGGTGCAGAAGt                                                         <  1:1311959/100‑1 (MQ=255)

                                       cGGCGTACTGATCTCTAACGGTCAGGGTAAAGCGGTCGCGTTCGCGCTGTTCGGTCTGTAGGATCGCGGTAAGCTGTTCCTCGGTCACGGTGCAGAAGtt                                                        >  1:2229636/1‑100 (MQ=255)

                                         gCGTACTGATCTCTAACGGTCAGGGTAAAGCGGTCGCGTTCGCGCTGTTCGGTCTGTAGGATCGCGGTAAGCTGTTCCTCGGTCACGGTGCAGAAGTTTa                                                      <  2:2972832/100‑1 (MQ=255)

                                          cGTACTGATCTCTAACGGTCAGGGTAAAGCGGTCGCGTTCGCGCTGTTCGGTCTGTAGGATCGCGGTAAGCTGTTCCTCGGTCACGGTGCAGAAGTTTAc                                                     <  1:767287/100‑1 (MQ=255)

                                           gTACTGATCTCTAACGGTCAGGGTAAAGCGGTCGCGTTCGCGCTGTTCGGTCTGTAGGATCGCGGTAAGCTGTTCCTCGGTCACGGTGCAGAAGTTTACg                                                    >  2:2592093/1‑100 (MQ=255)

                                            tACTGATCTCTAACGGTCAGGGTAAAGCGGTCGCGTTCGCGCTGTTCGGTCTGTAGGATCGCGGTAAGCTGTTCCTCGGTCACGGTGCAGAAGTTTACGa                                                   >  1:2483398/1‑100 (MQ=255)

                                             aCTGATCTCTAACGGTCAGGGTAAAGCGGTCGCGTTCGCGCTGTTCGGTCTGTAGGATCGCGGTAAGCTGTTCCTCGGTCACGGTGCAGAAGTTTACGaa                                                  <  2:173407/100‑1 (MQ=255)

                                              cTGATCTCTAACGGTCAGGGTAAAGCGGTCGCGTTCGCGCTGTTCGGTCTGTAGGATCGCGGTAAGCTGTTCCTCGGTCACGGTGCAGAAGTTTACGAAg                                                 >  1:998351/1‑100 (MQ=255)

                                              cTGATCTCTAACGGTCAGGGTAAAGCGGTCGCGTTCGCGCTGTTCGGTCTGTAGGATCGCGGTAAGCTGTTCCTCGGTCACGGTGCAGAAGTTTACGAAg                                                 >  1:1148944/1‑100 (MQ=255)

                                              cTGATCTCTAACGGTCAGGGTAAAGCGGTCGCGTTCGCGCTGTTCGGTCTGTAGGATCGCGGTAAGCTGTTCCTCGGTCACGGTGCAGAAGTTTACGAAg                                                 >  2:26250/1‑100 (MQ=255)

                                              cTGATCTCTAACGGTCAGGGTAAAGCGGTCGCGTTCGCGCTGTTCGATCTGTAGGATCGCGGTAAGCTGTTCCTCGGTCACGGTGCAGAAGTTTACGAAg                                                 >  1:1700016/1‑100 (MQ=255)

                                               tGATCTCTAACGGTCAGGGTAAAGCGGTCGCGTTCGCGCTGTTCGGTCTGTAGGATCGCGGTAAGCTGTTCCTCGGTCACGGTGCAGAAGTTTACGAAgg                                                >  2:605936/1‑100 (MQ=255)

                                                gATCTCTAACGGTCAGGGTAAAGCGGTCGCGTTCGCGCTGTTCGGTCTGTAGGATCGCGGTAAGCTGTTCCTCGGTCACGGTGCAGAAGTTTACGAAGGt                                               >  2:240091/1‑100 (MQ=255)

                                                gATCTCTAACGGTCAGGGTAAAGCGGTCGCGTTCGCGCTGTTCGGTCTGTAGGATCGCGGTAAGCTGTTCCTCGGTCACGGTGCAGAAGTTTACGAAGGt                                               >  2:1613674/1‑100 (MQ=255)

                                                   ctctAACGGTCAGGGTAAAGCGGTCGCGTTCGCGCTGTTCGGTCTGTAGGATCGCGGTAAGCTGTTCCTCGGTCACGGTGCAGAAGTTTACGAAGGTCAg                                            <  1:198082/100‑1 (MQ=255)

                                                   ctctAACGGTCAGGGTAAAGCGGTCGCGTTCGCGCTGTTCGGTCTGTAGGATCGCGGTAAGCTGTTCCTCGGTCACGGTGCAGAAGTTTACGAAGGTCAg                                            >  1:1902115/1‑100 (MQ=255)

                                                      ccgctctCAGGGTAAAGCGGTCGCGTTCGCGCTGTTCGGTCTGTAGGATCGCGGTAAGCTGTTCCTCGGTCACGGTGCAGAAGTTTACGAAGGTCAGAtt                                         <  1:563450/94‑1 (MQ=255)

                                                       aaCGGTCAGGGTAAAGCGGTCGCGTTCGCGCTGTTCGGTCTGTAGGATCGCGGTAAGCTGTTCCTCGGTCACGGTGCAGAAGTTTACGAAGGTCAGATTa                                        >  2:2675501/1‑100 (MQ=255)

                                                         cGGTCAGGGTAAAGCGGTCGCGTTCGCGCTGTTCGGTCTGTAGGATCGCGGTAAGCTGTTCCTCGGTCACGGTGCAGAAGTTTACGAAGGTCAGATTATc                                      <  1:2988860/100‑1 (MQ=255)

                                                         cGGTCAGGGTAAAGCGGTCGCGTTCGCGCTGTTCGGTCTGTAGGATCGCGGTAAGCTGTTCCTCGGTCACGGTGCAGAAGTTTACGAAGGTCAGATTATc                                      <  1:2088661/100‑1 (MQ=255)

                                                          ggTCAGGGTAAAGCGGTCGCGTTCGCGCTGTTCGGTCTGTAGGATCGCGGTAAGCTGTTCCTCGGTCACGGTGCAGAAGTTTACGAAGGTCAGATTATCg                                     <  1:2432995/100‑1 (MQ=255)

                                                            tCAGGGTAAAGCGGTCGCGTTCGCGCTGTTCGGTCTGTAGGATCGCGGTAAGCTGTTCCTCGGTCACGGTGCAGAAGTTTACGAAGGTCAGATTATCGGt                                   <  2:1501845/100‑1 (MQ=255)

                                                            tCAGGGTAAAGCGGTCGCGTTCGCGCTGTTCGGTCTGCAGGATCGCGGTAAGCTGTTCCTCGGTCACGGTGCAGAAGTTTACGAAGGTCAGATTATCGGt                                   <  2:1656347/100‑1 (MQ=255)

                                                             cAGGGTAAAGCGGTCGCGTTCGCGCTGTTCGGTCTGTAGGATCGCGGTAATCTGTTCCTCGGTCACGGTGCAGAAGTTTACGAAGGTCAGATTATCGGTa                                  <  1:2595777/100‑1 (MQ=255)

                                                             cAGGGTAAAGCGGTCGCGTTCGCGCTGTTCGGTCTGTAGGATCGCGGTAAGCTGTTCCTCGGTCACGGTGCAGAAGTTTACGAAGGTCAGATTagatcgg                                  >  2:563452/1‑94 (MQ=255)

                                                             cAGGGTAAAGCGGTCGCGTTCGCGCTGTTCGGTCTGTAGGATCGCGGTAAGCTGTTCCTCGGTCACGGTGCAGAAGTTTACGAAGGTCAGATTATCGGTa                                  >  1:1347267/1‑100 (MQ=255)

                                                              aGGGTAAAGCGGTCGCGTTCGCGCTGTTCGGTCTGTAGGATCGCGGTAAGCTGTTCCTCGGTCACGGTGCAGAAGTTTACGAAGGTCAGATTATCGGTAt                                 >  2:452195/1‑100 (MQ=255)

                                                               gggTAAAGCGGTCGCGTTCGCGCTGTTCGGTCTGTAGGATCGCGGTAAGCTGTTCCTCGGTCACGGTGCAGAAGTTTACGAAGGTCAGATTATCGGTAtt                                >  1:2676399/1‑100 (MQ=255)

                                                                ggTAAAGCGGTCGCGTTCGCGCTGTTCGGTCTGTAGGATCGCGGTAAGCTGTTCCTCGGTCACGGTGCAGAAGTTTACGAAGGTCAGATTATCGGTATTc                               >  2:2497584/1‑100 (MQ=255)

                                                                 gTAAAGCGGTCGCGTTCGCGCTGTTCGGTCTGTAGGATCGCGGTAAGCTGTTCCTCGGTCACGGTGCAGAAGTTTACGAAGGTCAGATTATCGGTATTCa                              <  1:1331980/100‑1 (MQ=255)

                                                                 gTAAAGCGGTCGCGTTCGCGCTGTTCGGTCTGTACGATCGCGGTAAGCTGTTCCTCGGTCACGGTGCAGAAGTTTACGAAGGTCAGATTATCGGTATTCa                              <  1:1613667/100‑1 (MQ=255)

                                                                   aaaGCGGTCGCGTTCGCGCTGTTCGGTCTGTAGGATCGCGGTAAGCTGTTCCTCGGTCACGGTGCAGAAGTTTACGAAGGTCAGATTATCGGTATTCATa                            <  2:931470/100‑1 (MQ=255)

                                                                     aGCGGTCGCGTTCGCGCTGTTCGGTCTGTAGGATCGCGGTAAGCTGTTCCTCGGTCACGGTGCAGAAGTTTACGAAGGTCAGATTATCGGTATTCATAGc                          <  1:2254861/100‑1 (MQ=255)

                                                                     aGCGGTCGCGTTCGCGCTGTTCGGTCTGTAGGATCGCGGTAAGCTGTTCCTCGGTCACGGTGCAGAAGTTTACGAAGGTCAGATTATCGGTATTCATAGc                          <  1:2810539/100‑1 (MQ=255)

                                                                      gCGGTCGCGTTCGCGCTGTTCGGTCTGTAGGATCGCGGTAAGCTGTTCCTCGGTCACGGTGCAGAAGTTTACGAAGGTCAGATTATCGGTATTCATAGcc                         <  2:1675018/100‑1 (MQ=255)

                                                                      gCGGTCGCGTTCGCGCTGTTCGGTCTGTAGGATCGCGGTAAGCTGTTCCTCGGTCACGGTGCAGAAGTTTACGAAGGTCAGATTATCGGTATTCATAGcc                         <  2:267419/100‑1 (MQ=255)

                                                                        ggTCGCGTTCGCGCTGTTCGGTCTGTAGGATCGCGGTAAGCTGTTCCTCGGTCACGGTGCAGAAGTTTACGAAGGTCAGATTATCGGTATTCATAGCCGc                       >  1:829237/1‑100 (MQ=255)

                                                                        ggTCGCGTTCGCGCTGTTCGGTCTGTAGGATCGCGGTAAGCTGTTCCTCGGTCAAGGTGCAGAAGTTTACGAAGGCCCGATTCTCGGTATCCATAGcaga                       >  2:563412/1‑97 (MQ=255)

                                                                         gTCGCGTTCGCGCTGTTCGGTCTGTAGGATCGCGGTAAGCTGTTCCTCGGTCACGGTGCAGAAGTTTACGAAGGTCAGATTATCGGTATTCATAGCCGct                      >  2:1482238/1‑100 (MQ=255)

                                                                         gTCGCGTTCGCGCTGTTCGGTCTGCAGGATCGCGGTAAGCTGTTCCTCGGTCACGGTGCAGAAGTTTACGAAGGTCAGATTATCGGTATTCATAGCCGct                      >  2:1649748/1‑100 (MQ=255)

                                                                            gcgTTCGCGCTGTTCGGTCTGTAGGATCGCGGTAAGCTGTTCCTCGGTCACGGTGCAGAAGTTTACGAAGGTCAGATTATCGGTATTCATAGCCGCTCTa                   <  1:1329678/100‑1 (MQ=255)

                                                                              gTTCGCGCTGTTCGGTCTGTAGGATCGCGGTAAGCTGTTCCTCGGTCACGGTGCAGAAGTTTACGAAGGTCAGATTATCGGTATTCATAGCCGCTCTAAc                 >  2:667901/1‑100 (MQ=255)

                                                                              gTTCGCGCTGTTCGGTCTGTAGGATCGCGGTAAGCTGTTACTCGGTCACGGTGCAGAAGTTTACGAAGGTCAGATTATCGGTATTCATAGCCGCTCTAAc                 <  1:1159495/100‑1 (MQ=255)

                                                                              cctggcgcTGTTCGGTCTGTAGGATCGCGGTAAGCTGTTCCTCGGTCACGGTGCAGAAGTTTACGAAGGTCAGATTATCGGTATTCATAGCCGCTCTAAc                 >  2:1560369/5‑100 (MQ=255)

                                                                               ttCGCGCTGTTCGGTCTGTAGGATCGCGGTAAGCTGTTCCTCGGTCACGGTGCAGAAGTTTACGAAGGTCAGATTATCGGTATTCATAGCCGCTCTAACg                <  1:240091/100‑1 (MQ=255)

                                                                               ttCGCGCTGTTCGGTCTGTAGGATCGCGGTAAGCTGTTCCTCGGTCACGGTGCAGAAGTTTACGAAGGTCAGATTATCGGTATTCATAGCCGCTCTAACg                >  2:2285332/1‑100 (MQ=255)

                                                                                  tccgatctCGGTCTGTAGGATCGCGGTAAGCTGTTCCTCGGTCACGGTGCAGAAGTTTACGAAGGTCAGATTATCGGTATTCATAGCCGCTCTAACGAcc             <  2:1332150/93‑1 (MQ=255)

                                                                                   cgcTGTTCGGTCTGTAGGATCGCGGTAAGCTGTTCCTCGGTCACGGTGCAGAAGTTTACGAAGGTCAGATAATCGGTATTCATAGCCGCTCTAACGACCt            >  2:2863545/1‑100 (MQ=255)

                                                                                    gcTGTTCGGTCTGTAGGATCGCGGTAAGCTGTTCCTCGGTCACGGTGCAGAAGTTTACGAAGGTCAGATTATCGGTATTCATAGCCGCTCTAACGACCTg           <  1:452193/100‑1 (MQ=255)

                                                                                    gcTGTTCGGTCTGTAGGATCGCGGTAAGCTGTTCCTCGGTCACGGTGCAGAAGTTTACGAAGGTCAGATTATCGGTATTCATAGCCGCTCTAACGACCTg           <  1:1900755/100‑1 (MQ=255)

                                                                                    gcTGTTCGGTCTGTAGGATCGCGGTAAGCTGTTCCTCGGTCACGGTGCAGAAGTTTACGAAGGTCAGATTATCGGTATTCATAGCCGCTCTAACGACCTg           <  1:142635/100‑1 (MQ=255)

                                                                                     cTGTTCGGTCTGTAGGATCGCGGTAAGCTGTTCCTCGGTCACGGTGCAGAAGTTTACGAAGGTCAGATTATCGGTATTCATAGCCGCTCTAACGACCTGa          >  2:1295942/1‑100 (MQ=255)

                                                                                       gTTCGGTCTGTAGGATCGCGGTAAGCTGTTCCTCGGTCACGGTGCAGAAGTTTACGAAGGTCAGATTATCGGTATTCATAGCCGCTCTAACGACCTGACt        >  1:389479/1‑100 (MQ=255)

                                                                                       gTTCCGTCTGTAGGATCGCGGTAAGCTGTTCCTCGGTCACGGTGCAGAAGTTTACGAAGGTCAGCTTATCGGTATTCATAGCCGCTCTAACGACCTGACt        >  1:389359/1‑100 (MQ=255)

                                                                                           ggTCTGTAGGATCGCGGTAAGCTGTTCCTCGGTCACGGTGCAGAAGTTTACGAAGGTCAGATTATCGGTATTCATAGCCGCTCTAACGACCTGACTGTaa    >  1:464159/1‑100 (MQ=255)

                                                                                             tCTGTAGGATCGCGGTAAGCTGTTCCTCGGTCACGGTGCAGAAGTTTACGAAGGTCAGATTATCGGTATTCATAGCCGCTCTAACGACCTGACTGTAAAc  <  2:318496/100‑1 (MQ=255)

                                                                                             tCTGTAGGATCGCGGTAAGCTGTTCCTCGGTCACGGTGCAGAAGTTTACGAAGGTCAGATTATCGGTATTCATAGCCGCTCTAACGACCTGACTGTAAAc  <  1:2465003/100‑1 (MQ=255)

                                                                                             tCTGTAGGATCGCGGTAAGCTGTTCCTCGGTCACGGTGCAGAAGTTTACGAAGGTCAGATTATCGGTATTCATAGCCGCTCTAACGACCTGACTGTAAAc  <  1:2106161/100‑1 (MQ=255)

                                                                                             tCTGTAGGATCGCGGTAAGCTGTTCCTCGGTCACGGTGCAGAAGTTTACGAAGGTCAGATTATCGGTATTCATAGCCGCTCTAACGACCTGACTGTAAAc  <  2:1625862/100‑1 (MQ=255)

CGACGACGTACGTCCGGGTGAAGTGGGTCAGCGTCAGAACGGCGTACTGATCTCTAACGGTCAGGGTAAAGCGGTCGCGTTCGCGCTGTTCGGTCTGCAGGATCGCGGTAAGCTGTTCCTCGGTCACGGTGCAGAAGTTTACGAAGGTCAGATTATCGGTATTCATAGCCGCTCTAACGACCTGACTGTAAAC  >  NC_000913/4059822‑4060014

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`