BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,397,557	G→T	G49V (GGT→GTT)	mutL →	methyl‑directed mismatch repair protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,397,557	0	G	T	100.0%	377.2 / NA	109	G49V (GGT→GTT)	mutL	methyl‑directed mismatch repair protein
Reads supporting (aligned to +/- strand): ref base G (0/0); new base T (50/59); total (50/59)

Only 100 of 109 total aligned reads displayed.

GGTGAGGTGGTCGAGCGACCTGCGTCGGTAGTCAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGA‑ACGCGGTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATGAGCTGGCGCTGGCGCTGGCTCGTCATGCCACCAGTAAAATCG  >  NC_000913/4397460‑4397652

ggtgaggtgGTCGAGCGACCTGCGTCGGTAGTCAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGA‑ACGCGTTg                                                                                               <  1:243188/100‑1 (MQ=255)

ggtgaggtgGTCGAGCGACCTGCGTCGGTAGTCAAAGAACTAGGGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGA‑ACGCGTTg                                                                                               <  1:1958238/100‑1 (MQ=255)

 gtgaggtgGTCGGGCGACCTGCGTCGGTAGTCAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGA‑ACGCGTTgg                                                                                              <  1:1467719/100‑1 (MQ=255)

 gtgaggtgGTCGAGCGACCTGCGTCGGTAGTCAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGA‑ACGCGTTgg                                                                                              >  2:2259639/1‑100 (MQ=255)

   gaggtgGTCGAGCGACCTGCGTCGGTAGTCAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGA‑ACGCGTTgggg                                                                                            >  2:1867022/1‑100 (MQ=255)

   gaggtgGTCGAGCGACCTGCGTCGGTAGTCAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGA‑ACGCGTTgggg                                                                                            >  1:1370840/1‑100 (MQ=255)

   gaggtgGTCGAGCGACCTGCGTCGGTAGTCAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGA‑ACGCGTTgg                                                                                              <  2:2432241/98‑1 (MQ=255)

    aggtgGTCGAGCGACCTGCGTCGGTAGTCAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGA‑ACGCGTTGGGGc                                                                                           <  1:1146112/100‑1 (MQ=255)

     ggtggtCGAGCGACCTGCGTCGGTAGTCAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGA‑ACGCGTTGGGGCg                                                                                          >  2:656083/1‑100 (MQ=255)

     ggtggtCGAGCGACCTGCGTCGGTAGTCAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGA‑ACGCGTTGGGGCg                                                                                          >  1:1066181/1‑100 (MQ=255)

     ggtggtCGAGCGACCTGCGTCGGTAGTCAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGA‑ACGCGTTGGGGCg                                                                                          >  2:2639620/1‑100 (MQ=255)

         gtCGAGCGTCCTGCTTCGGTAGTCACAGAACTAGTTAAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGA‑ACGCGTTGGGTCGAAAc                                                                                      <  1:2756472/100‑1 (MQ=255)

         gtCGAGCGACCTGCGTCGGTAGTCAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGA‑ACGCGTTGGGGCGAAAc                                                                                      <  2:1393319/100‑1 (MQ=255)

          tCGAGCGACCTGCGTCGGTAGTCAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGA‑ACGCGTTGGGGCGAAACt                                                                                     >  2:1610259/1‑100 (MQ=255)

            gAGCGACCTGCGTCGGTAGTCAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGA‑ACGCGTTGGGGCGAAACTTa                                                                                   <  1:2992712/100‑1 (MQ=255)

            gAGCGACCTGCGTCGGTAGTCAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGA‑ACGCGTTGGGGCGAAACTTa                                                                                   >  2:2685976/1‑100 (MQ=255)

               cGACCTGCGTCGGTAGTCAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGA‑ACGCGTTGGGGCGAAACTTATcc                                                                                <  1:1031080/100‑1 (MQ=255)

               cGACCTGCGTCGGTAGTCAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGA‑ACGCGTTGGGGCGAAACTTATcc                                                                                <  2:2493343/100‑1 (MQ=255)

                 aCCTGCGTCGGTAGTCAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGc                                                                              >  2:2267373/1‑100 (MQ=255)

                 aCCTGCGTCGGTAGTCAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGc                                                                              >  2:339354/1‑100 (MQ=255)

                   cTGCGTCGGTAGTCAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCAt                                                                            <  2:2189282/100‑1 (MQ=255)

                    tGCGTCGGTAGTCAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCAtt                                                                           >  2:1183913/1‑100 (MQ=255)

                    tGCGTCGGTAGTCAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCAtt                                                                           <  1:729453/100‑1 (MQ=255)

                        tCGGTAGTCAAAGAACTAGTGGAAAACAGCCTCGATGCAGGGGCGACGCGTATCGATATTGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTg                                                                       <  1:1101320/100‑1 (MQ=255)

                         cGGTAGTCAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGa                                                                      <  1:2196066/100‑1 (MQ=255)

                          ggTAGTCAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGAt                                                                     >  2:1141220/1‑100 (MQ=255)

                          ggTAGTCAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGAt                                                                     <  2:2412578/100‑1 (MQ=255)

                               tCAAAGAACTAGCGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACgg                                                                >  2:2007509/1‑100 (MQ=255)

                                cAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGc                                                               <  2:2387864/100‑1 (MQ=255)

                                cAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGc                                                               >  2:1229736/1‑100 (MQ=255)

                                cAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGc                                                               <  2:1217943/100‑1 (MQ=255)

                                cAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGc                                                               >  1:2897058/1‑100 (MQ=255)

                                cAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGc                                                               >  1:152892/1‑100 (MQ=255)

                                  aaGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTCATAACGGCt                                                              >  1:615960/1‑99 (MQ=255)

                                   aGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGc                                                            <  1:1351716/100‑1 (MQ=255)

                                    gagcgaGTGGAAAACAGTCGGGGTTCATGCGCGACTGGTATCGATATTGATATCTA‑ACGCGTTGTGGCGAAACTGAGCCGCATTCGTGATAACGGc                                                               <  1:2830937/91‑1 (MQ=255)

                                     aaCTAGTCGAAAACAGACTCGATGCCGGTGCGACGCGTATCGATATTGATATCGA‑CCGCGTTGGGGCGAAACTTATCCGCATTTGTGATAACGGCTGctg                                                          <  1:1017439/100‑3 (MQ=255)

                                        tAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTAt                                                       <  2:2951250/100‑1 (MQ=255)

                                         aGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATc                                                      <  1:1169175/100‑1 (MQ=255)

                                          gTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCa                                                     >  2:2485816/1‑100 (MQ=255)

                                           tGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCaa                                                    >  2:2606281/1‑100 (MQ=255)

                                            ggAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCaaa                                                   >  1:2857956/1‑100 (MQ=255)

                                            ggAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCaaa                                                   <  1:1918031/100‑1 (MQ=255)

                                            ggAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCaaa                                                   <  2:422/100‑1 (MQ=255)

                                            ggAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCaaa                                                   >  1:987845/1‑100 (MQ=255)

                                              aaaaCAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCaaaaa                                                 >  2:2577010/1‑100 (MQ=255)

                                              aaaaCAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCaaaaa                                                 <  1:765985/100‑1 (MQ=255)

                                               aaaCAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCaaaaaa                                                <  1:592885/100‑1 (MQ=255)

                                                aaCAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAg                                               <  2:1403510/100‑1 (MQ=255)

                                                 aCAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGAGGAAAAAAAAAAg                                               <  1:2439241/99‑1 (MQ=255)

                                                   cttccgatctcAGGTGCGACGCGTATCGATATTGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATg                                            <  2:2317108/90‑1 (MQ=255)

                                                   aGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGacg                                            <  2:2835309/100‑3 (MQ=255)

                                                   aGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGAt                                             <  2:677653/99‑1 (MQ=255)

                                                     ccTCGATGCAGGTGCGACGCGTATCGATATTGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATGAg                                          >  1:1855917/1‑100 (MQ=255)

                                                      cTCGATGCAGGTGCGACGCGTATCGATATCGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCAGTATCAAAAAAGATGAgc                                         <  1:2340290/100‑1 (MQ=255)

                                                        cGATCCAGGTGCGACGCGAATCGAGATTGATAGCGA‑ACGCGTGGGGGCGAAACTTAGCCGCATGCGTTAGAACGTCTGCGGTATCAAAAAAGATTAgctg                                       >  1:2557539/1‑100 (MQ=255)

                                                         gATGCAGGTGCGACGCGTATCGATATTGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATGAgctgg                                      >  2:1431609/1‑100 (MQ=255)

                                                          aTGCAGGTGCGAGGCGGATAGGTATTGATAGCGA‑ACGCGTGGGGTCGAAGCTTATCCGCGTGCGTAATAAAAGAAGCGGTATCAAAAAAGATGAgctggc                                     <  2:8165/100‑1 (MQ=255)

                                                          aTGCAGGTGCGACGCGTATCGATATTGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATGAgctggc                                     <  2:1066185/100‑1 (MQ=255)

                                                           tGCAGGGTCGACGCGCATCGATATTGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATGAgctg                                       <  2:2557553/97‑1 (MQ=255)

                                                            gCAGGTGCGACGCGTATCGATATTGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATGAgctggcgc                                   <  2:520186/100‑1 (MQ=255)

                                                             cAGGTGCGACGCGTATCGATATTGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATGAgctggcgct                                  >  1:1476582/1‑100 (MQ=255)

                                                             cAGGTGCGACGCGTATCGATATTGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATGAgctggcgct                                  <  2:512239/100‑1 (MQ=255)

                                                             cAGGTGCGACGCGTATCGATATTGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATGAgatcggaag                                  >  1:2317099/1‑92 (MQ=255)

                                                              aGGTGCGACGCGTATCGATATTGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATGAgctggcgctg                                 <  2:1454401/100‑1 (MQ=255)

                                                              aGGTGCGACGCGTATCGATATTGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATGAgctggcgctg                                 >  1:2143736/1‑100 (MQ=255)

                                                               ggTGCGACGCGTATCGATATTGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATGAgctggcgctgg                                <  1:581333/100‑1 (MQ=255)

                                                               ggTGCGACGCGTATCGATATTGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATGAgctggcgctgg                                <  1:1229732/100‑1 (MQ=255)

                                                               ggTGCGACGCGTATCGATATTGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATGAgctggcgctgg                                >  2:422741/1‑100 (MQ=255)

                                                                  gCGACGCGTATCGATATTGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATGAgctggcgctggc                               <  1:2810013/98‑1 (MQ=255)

                                                                    gACGCGTATCGATATTGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTAACAAAAAAGATGAgctggcgctggcgctg                           <  1:1899225/100‑1 (MQ=255)

                                                                      cgcgTATCGATATTGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATGAgttggcgctggcgctgg                          >  1:853785/1‑99 (MQ=255)

                                                                      cgcgTATCGATATTGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATGAgctggcgctggcgctggc                         <  2:1783399/100‑1 (MQ=255)

                                                                      cgcgTATCGATATTGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATGAgctggcgctggcgctggc                         >  2:1444492/1‑100 (MQ=255)

                                                                      cgcgTATCGATATTGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATGAgctggcgctggcgctggc                         >  1:715102/1‑100 (MQ=255)

                                                                      cgcgTATCGATATTGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATGAgctggcgctggcgctggc                         >  1:623044/1‑100 (MQ=255)

                                                                      cgcgTATCGATATTGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATGAgctggcgctggcgctg                           <  1:1341490/98‑1 (MQ=255)

                                                                       gcgTATCGATATTGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATGAGCTGGCGCTGGCGCTGGCt                        <  2:702269/100‑1 (MQ=255)

                                                                       cgcgttCGATATCAATATCGATACGCGTCGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATGAgctggcgctggcgctggc                         >  2:81665/6‑100 (MQ=25)

                                                                        cgTATCGATATTGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATGAGATGGCGCTGGCgctcgctc                       >  1:2334186/1‑100 (MQ=255)

                                                                          tATCGATATTGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATGAGCTGGCGCTGGCGCTGGCTCGt                     <  1:2685960/100‑1 (MQ=255)

                                                                           aTCGATATTGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATGAGCTGGCGCTGGCGCTGGCTCGTc                    <  1:630898/100‑1 (MQ=255)

                                                                            tCGATATTGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATGAGCTGGCGCTGGCGCTGGCTCGTCa                   <  2:2131507/100‑1 (MQ=255)

                                                                              gATATTGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATGAGCTGGCGCTGGCGCTGGCTCGTCATg                 >  2:1678800/1‑100 (MQ=255)

                                                                              gATATTGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATGAGCTGGCGCTGGCGCTGGCTCGTCATg                 >  1:1540994/1‑100 (MQ=255)

                                                                                tatTGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATGAGCTGGCGCTGGCGCTGGCTCGTCATGcc               <  2:2883968/100‑1 (MQ=255)

                                                                                 atTGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATGAGCTGGCGCTGGCGCTGGCTCGTCATGcca              >  2:1727254/1‑100 (MQ=255)

                                                                                   tGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATGAGCTGGCGCTGGCGCTGGCTCGTCATGccacc            <  2:2546542/100‑1 (MQ=255)

                                                                                   tGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATGAGCTGGCGCTGGCGCTGGCTCGTCATGccacc            >  1:1041591/1‑100 (MQ=255)

                                                                                   tGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATGAGCTGGCGCTGGCGCTGGCTCGTCATGccacc            >  1:1041627/1‑100 (MQ=255)

                                                                                   tGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATGAGCTGGCGCTGGCGCTGGCTCGTCATGccacc            >  1:1757698/1‑100 (MQ=255)

                                                                                   tGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATGAGCTGGCGCTGGCGCTGGCTCGTCATGccacc            <  1:2164715/100‑1 (MQ=255)

                                                                                   tGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATGAGCTGGCGCTGGCGCTGGCTCGTCATGccacc            <  2:1545409/100‑1 (MQ=255)

                                                                                   tGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATGAGCTGGCGCTGGCGCTGGCTCGTCATGcca              >  1:1939435/1‑98 (MQ=255)

                                                                                    gATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATGAGATGGCGCTGGCGCTGGCTCGTCATGccacca           >  1:494451/1‑100 (MQ=255)

                                                                                     atatCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATGAGCTGGCGCTGGCGCTGGCTCGTCATGCCACCAg          >  2:1000334/1‑100 (MQ=255)

                                                                                     atatCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATGAGCTGGCGCTGGCGCTGGCTCGTCATGCCACCAg          <  2:742533/100‑1 (MQ=255)

                                                                                     atatCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATGAGCTGGCGCTGGCGCTGGCTCGTCATGCCACCAg          <  2:2506512/100‑1 (MQ=255)

                                                                                          gA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATGAGCTGGCGCTGGCGCTGGCTCGTCATGCCACCAGTaaaa     >  1:1585404/1‑100 (MQ=255)

                                                                                              cgcgTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATGAGCTGGCGCTGGCGCTGGCTCGTCATGCCACCAGTAAAATCg  <  2:1314081/100‑1 (MQ=255)

GGTGAGGTGGTCGAGCGACCTGCGTCGGTAGTCAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGA‑ACGCGGTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATGAGCTGGCGCTGGCGCTGGCTCGTCATGCCACCAGTAAAATCG  >  NC_000913/4397460‑4397652

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`