Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,215,256 T→C I74V (ATT→GTT)  bluF ← anti‑repressor for YcgE, blue light‑responsive; FAD‑binding; inactive c‑di‑GMP phosphodiesterase‑like EAL domain protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,215,2560TC97.2% 375.9 / ‑4.2 106I74V (ATT→GTT) bluFanti‑repressor for YcgE, blue light‑responsive; FAD‑binding; inactive c‑di‑GMP phosphodiesterase‑like EAL domain protein
Reads supporting (aligned to +/- strand):  ref base T (2/1);  new base C (61/42);  total (63/43)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 113 total aligned reads displayed.

AAAACGTCATCTCGCTCGTGCAGGCGCAAATCAAATAATTCCATTCCCGCTTTGCCAAAACGGCGAGCAGGCGCGTAATCGCACAGCAGCTCAACAATATTATAGTGCCGTGGATCCTGGCATATAGCCCGATATATCATTTTAACCTGTTCTTCCGGACCTTCCAGAAGCTGGAAAAAATGAGAACCATTAAA  >  NC_000913/1215159‑1215352
                                                                                                 |                                                                                                
aaaaCGTCATCTCGCTCGTGCAGGCGCAAATCAAATAATTCCATTCCCGCTTTGCCAAAACGGCGAGCAGGCGCGTAATCGCACAGCAGCTCAACAacat                                                                                                <  2:815500/100‑1 (MQ=255)
     gTCATCTCGCTGGTGCAGGCGCAAATCAAATAATTCCATTCCCGCTTGGCAAAACCGGCGAGCAGGCGCGGAATCGCACAGCAGCTCAACAACATTATAg                                                                                           <  2:1145573/100‑1 (MQ=255)
      tCATCTCGCTCGTGCAGGCGCAAATCAAATAATTCCATTCCCGCTTTGCCAAAACGGCGAGCAGGCGCGTAATCGCACAGCAGCTCAACAACATTATAGt                                                                                          >  1:2162648/1‑100 (MQ=255)
        aTCTCGCTCGTGCAGGCGCAAATCAAATAATTCCATTCCCGCTTTGCCAACACGGCGAGCAGGCGCGTAATCGAACAGCAGCACAACAACATTCTAGt                                                                                          >  1:2145030/1‑98 (MQ=255)
         tctcGCTCGTGCAGGCGCAAATCAAATAATTCCATTCCCGCTTTGCCAAAACGGCGAGCAGGCGCGTAATCGCACAGCAGCTCAACAACATTATAGTGcc                                                                                       >  2:1327871/1‑100 (MQ=255)
         tctcGCTCGTGCAGGCGCAAATCAAATAATTCCATTCCCGCTTTGCCAAAACGGCGAGCAGGCGCGTAATCGCACAGCAGCTCAACAACATTATAGTGcc                                                                                       >  2:2037967/1‑100 (MQ=255)
            cgctcgTGCAGGCGCAAATCAAATAATTCCATTCCCGCTTTGCCAAAACGGCGAGCAGGCGCGTAATCGCACAGCAGCTCAACAACATTATAGTGCCGTg                                                                                    <  1:136499/100‑1 (MQ=255)
              ctcgTGCAGGCGCAAATCAAATAATTCCATTCCCGCTTTGCCAAAACGGCGAGCAGGCGCGTAATCGCACAGCAGCTCAACAACATTATAGTGCCGTgg                                                                                   >  2:1774118/1‑99 (MQ=255)
              ctcgTGCAGGCGCAAATCAAATAATTCCATTCCCGCTTTGCCAAAACGGCGAGCAGGCGCGTAATCGCACAGCAGCTCAACAACATTATAGTGCCGTGGa                                                                                  >  2:1774311/1‑100 (MQ=255)
                cgTGCAGGCGCAAATCAAATAATTCCATTCCCGCTTTGCCAAAACGGCGAGCAGGCGCGTAATCGCACAGCAGCTCAACAACATTATAGTGCCGTGGATc                                                                                >  2:2778028/1‑100 (MQ=255)
                 gTGCAGGCGCAAATCAAATAATTCCATTCCCGCTTTGCCAAAACGGCGAGCAGGCGCGTAATCGCACAGCAGCTCAACAACATTATAGTGCCGTGGATcc                                                                               <  2:294911/100‑1 (MQ=255)
                  tGCAGGCGCAAATCAAATAATTCCATTCCCGCTTTGCCAAAACGGCGAGCAGGCGCGTAATCGCACAGCAGCTCAACAACATTATAGTGCCGTGGATCCt                                                                              >  2:2608537/1‑100 (MQ=255)
                     aGGCGCAAATCAAATAATTCCATTCCCGCTTTGCCAAAACGGCGAGCAGGCGCGTAATCGCACAGCAGCTCAACAACATTATAGTGCCGTGGATCCTGGc                                                                           >  1:2462859/1‑100 (MQ=255)
                      ggCGCAAATCAAATAATTCCATTCCCGCTTTGCCAAAACGGCGAGCAGGCGCGTAATCGCACAGCAGCTCAACAACATTATAGTGCCGTGGATCCTGGCa                                                                          <  2:1333676/100‑1 (MQ=255)
                      ggCGCAAATCAAATAATTCCATTCCCGCTTTGCCAAAACGGCGAGCAGGCGCGTAATCGCACAGCAGCTCAACAACATTATAGTGCCGTGGATCCTGGCa                                                                          <  2:59829/100‑1 (MQ=255)
                      ggCGCAAATCAAATAATTCCATTCCCGCTTTGCCAAAACGGCGAGCAGGCGCGTAATCGCACAGCAGCTCAACAACATTATAGTGCCGTGGATCCTGGCa                                                                          >  2:2485923/1‑100 (MQ=255)
                        cgcAAATCAAATAATTCCATTCCCGCTTTGCCAAAACGGCGAGCAGGCGCGTAATCGCACAGCAGCTCAACAACATTATAGTGCCGTGGATCCTGGCata                                                                        <  1:995994/100‑1 (MQ=255)
                        cgcAAATCAAATAATTCCATTCCCGCTTTGCCAAAACGGCGAGCAGGCGCGTAATCGCACAGCAGCTCAACAACATTATAGTGCCGTGGATCCTGGCata                                                                        >  2:2624557/1‑100 (MQ=255)
                         gcAAATCAAATAATTCCATTCCCGCTTTGCCAAAACGGCGAGCAGGCGCGTAATCGCACAGCAGCTCAACAACATTATAGTGCCGTGGATCCTGGCatat                                                                       <  2:2504752/100‑1 (MQ=255)
                          cgatctaaatAATTCCATTCCCGCTTTGCCAAAACGGCGAGCAGGCGCGTAATCGCACAGCAGCTCAACAACATTATAGTGCCGTGGATCCTGGCAtata                                                                      <  1:1284151/94‑1 (MQ=255)
                             atcaaatAATTCCATTCCCGCTTTGCCAAAACGGCGAGCAGGCGCGTAATCGCACAGCAGCTCAACAACATTATAGTGCCGTGGATCCTGGCATATAGcc                                                                   >  2:2077855/1‑100 (MQ=255)
                              tcaaatAATTCCATTCCCGCTTTGCCAAAACGGCGAGCAGGCGCGTAATCGCACAGCAGCTCAACAACATTATAGTGCCGTGGATCCTGGCATATAGccc                                                                  <  1:1800686/100‑1 (MQ=255)
                               caaatAATTCCATTCCCGCTTTGCCAAAACGGCGAGCAGGCGCGTAATCGCACAGCAGCTCAACAACATTATAGTGCCGTGGATCCTGGCATATAGCCCg                                                                 <  1:2186092/100‑1 (MQ=255)
                               caaatAATTCCATTCCCGCTTTGCCAAAACGGCGAGCAGGCGCGTAATCGCACAGCAGCTCAACAACATTATAGTGCCGTGGATCCTGGCATATAGCCCg                                                                 <  2:2162658/100‑1 (MQ=255)
                                aaatAATTCCATTCCCGCTTTGCCAAAACGGCGAGCAGGCGCGTAATCGCACAGCAGCTCAACAACATTATAGTGCCGTGGATCCTGGCAtataagatcg                                                                >  2:1284155/1‑94 (MQ=255)
                                aaatAATTCCATTCCCGCTTTGCCAAAACGGCGAGCAGGCGCGTAATCGCACAGCAGCTCAACAACATTATAGTGCCGTGGATCCTGGCATATAGCCCGa                                                                >  2:2702380/1‑100 (MQ=255)
                                aaatAATTCCATTCCCGCTTTGCCAAAACGGCGAGCAGGCGCGTAATCGCACAGCAGCTCAACAACATTATAGTGCCGTGGATCCTGGCATATAGCCCGa                                                                >  1:635547/1‑100 (MQ=255)
                                 aataatTCCATTCCCGCTTTGCCAAAACGGCGAGCAGGCGCGTAATCGCACACCAGCTCACCAACATTATAGTGCCGTGGATCCTGGCATATAGCCCGa                                                                >  2:2152020/1‑99 (MQ=255)
                                  ataatTCCATTCCCGCTTTGCCAAAACGGCGAGCAGGCGCGTAATCGCACAGCAGCTCAACAACATTATAGTGCCGTGGATCCTGGCATATAGCCCGata                                                              <  2:1659890/100‑1 (MQ=255)
                                     attccattccCGCTTTGCCAAAACGGCGAGCAGGCGCGTAATCGCACAGCAGCTCAACAACATTATAGTGCCGTGGATCCTGGCATATAGCCCGatatat                                                           >  2:1928040/1‑100 (MQ=255)
                                      ttccattctCGCTTTGCTAGAGCGGCTAGCAGGCGCGTAATAGCACACTAGATCAACAACTTTATAGTGCCGTGGATCCTGGCATATAGCCCGATATATc                                                          <  1:2625186/100‑1 (MQ=255)
                                      ttccattccCGCTTTGCCAAAACGGCGAGCAGGCGCGTAATCGCACAGCAGCTCAACAACATTATAGTGCCGTGGATCCTGGCATATAGCCCGATATATc                                                          <  2:1138806/100‑1 (MQ=255)
                                        ccattccCGCTTTGCCAAAACGGCGAGCAGGCGCGTAATCGCACAGCAGCTCAACAACATTATAGTGCCGTGGATCCTGGCATATAGCCCGATATATCAt                                                        >  2:749919/1‑100 (MQ=255)
                                         cattccCGCTTTGCCAAACGGCGAGCAGGCGCGTAATCGCACAGCAGCTCAACAACATTATAGTGCCGTGGATCCTGGCATATAGCCCGATATATCAttt                                                      >  2:117693/1‑100 (MQ=255)
                                          attccCGCTTTGCCAAAACGGCGAGCAGGCGCGTAATCGCACAGCAGCTCAACAACATTATAGTGCCGTGGATCCTGGCATATAGCCCGATATATCAttt                                                      <  1:355117/100‑1 (MQ=255)
                                           ttccCGCTTTGCCAAAACGGCGAGCAGGCGCGTAATCGCACAGCAGCTCAACAATATTATAGTGCCGTGGATCCTGGCATATAGCCCGATATATCAtttt                                                     >  2:1006685/1‑100 (MQ=255)
                                           ttccCGCTTTGCCAAAACGGCGAGCAGGCGCGTAATCGCACAGCAGCTCAACAACATTATAGTGCCGTGGATCCTGGCATATAGCCCGATATATCAtttt                                                     >  1:329132/1‑100 (MQ=255)
                                             cccGCTTTGCCAAAACGGCGAGCAGGCGCGTAATCGCACAGCAGCTCAACAACATTATAGTGCCGTGGATCCTGGCATATAGCCCGATATATCATTTTaa                                                   >  2:116567/1‑100 (MQ=255)
                                             cccGCTTTGCCAAAACGGCGAGCAGGCGCGTAATCGCACAGCAGCTCAACAACATTATAGTGCCGTGGATCCTGGCATATAGCCCGATATATCATTTTaa                                                   >  2:2542704/1‑100 (MQ=255)
                                             cccGCTTTGCCAAAACGGCGAGCAGGCGCGTAATCGCACAGCAGCTCAACAACATTATAGTGCCGTGGATCCTGGCATATAGCCCGATATATCATTTTaa                                                   >  2:2390224/1‑100 (MQ=255)
                                               gatcttGCCAAAACGGCGAGCAGGCGCGTAATCGCACAGCAGCTCAACAACATTATAGTGCCGTGGATCCTGGCATATAGCCCGATATATCATTTTAAcc                                                 <  1:1470800/96‑1 (MQ=255)
                                               cGCTTTGCCAAAACGGCGAGCAGGCGCGTAATCGCACAGCAGCTCAACAACATTATAGTGCCGTGGATCCTGGCATATAGCCCGATATATCATTTTAAcc                                                 <  2:713242/100‑1 (MQ=255)
                                               cGCTTTGCCAAAACGGCGAGCAGGCGCGTAATCGCACAGCAGCTCAACAACATTATAGTGCCGTGGATCCTGGCATATAGCCCGATATATCATTTTAAcc                                                 <  1:1682182/100‑1 (MQ=255)
                                                gCTTTGCCAAAACGGCGAGCAGGCGCGTAATCGCACAGCAGCTCAACAACATTATAGTGCCGTGGATCCTGGCATATAGCCCGATATATCATTTTAACCt                                                >  2:1503990/1‑100 (MQ=255)
                                                gCTTTGCCAAAACGGCGAGCAGGCGCGTAATCGCACAGCAGCTCAACAACATTATAGTGCCGTGGATCCTGGCATATAGCCCGATATATCATTTTAACCt                                                >  2:1504184/1‑100 (MQ=255)
                                                gCTTTGCCAAAACGGCGAGCAGGCGCGTAATCGCACAGCAGCTCAACAACATTATAGTGCCGTGGATCCTGGCATATAGCCCGATATATCATTTTAACCt                                                >  2:935532/1‑100 (MQ=255)
                                                    tGCCAAAACGGCGAGCAGGCGCGTAATCGCACAGCAGCTCAACAACATTATAGTGCCGTGGATCCTGGCATATAGCCCGATATATCATTTTAACCTGttc                                            >  1:807644/1‑100 (MQ=255)
                                                      ccAAAACGGCGAGCAGGCGCGTAATCGCACAGCAGCTCAACAACATTATAGTGCCGTGGATCCTGGCATATAGCCCGATATATCATTTTAACCTGttctt                                          >  2:1437738/1‑100 (MQ=255)
                                                       cAAAACGGCGAGCAGGCGCGTAATCGCACAGCCGCTCAAGACTATTATAGTGCCGTGGATCCTGGCATATAGCCCGATATATCATTTTAACCTGttcttc                                         <  1:2152010/100‑1 (MQ=255)
                                                         aaaCGGCGAGCAGGCGCGTAATCGCACAGCAGCTCAACAACATTATAGTGCCGTGGATCCTGGCATATAGCCCGATATATCATTTTAACCTGTTCTTCCg                                       <  2:1835071/100‑1 (MQ=255)
                                                         aaaCGGCGAGCAGGCGCGTAATCGCACAGCAGCTCAACAACATTATAGTGCCGTGGATCCTGGCATATAGCCCGATATATCATTTTAACCTGTTCTTCCg                                       >  1:2374136/1‑100 (MQ=255)
                                                           aCGGCGAGCAGGCGCGTAATCGCACAGCAGCTCAACAACATTATAGTGCCGTGGATCCTGGCATATAGCCCGATATATCATTTTAACCTGTTCTTCCGGa                                     <  2:1476795/100‑1 (MQ=255)
                                                            cGGCGAGCAGGCGCGTAATCGCACAGCAGCTCAACAACATTATAGTGCCGTGGATCCTGGCATATAGCCCGATATATCATTTTAACCTGTTCTTCCGGAc                                    >  2:2823049/1‑100 (MQ=255)
                                                            cGGCGAGCAGGCGCGTAATCGCACAGCAGCTCAACAACATTATAGTGCCGTGGATCCTGGCATATAGCCCGATATATCATTTTAACCTGTTCTTCCGGAc                                    >  1:2192592/1‑100 (MQ=255)
                                                                gAGCAGGCGCGCAATCGCACAGCAGCTCAACAACATTATAGTGCCGTGGCTCCTGGCATATAGCCCGATATATCATTTTAACCTGTTCTTCCGGACCTTc                                >  1:1979692/1‑100 (MQ=255)
                                                                 aGCAGGCGCGTAATCGCACAGCAGCTCAACAACATTATAGTGCCGTGGATCCTGGCATATAGCCCGATATATCATTTTAACCTGTTCTTCCGGACCTTcc                               <  2:78138/100‑1 (MQ=255)
                                                                 aGCAGGCGCGTAATCGCACAGCAGCTCAACAACATTATAGTGCCGTGGATCCTGGCATATAGCCCGATATATCATTTTAACCTGTTCTTCCGGACCTTcc                               <  2:2230667/100‑1 (MQ=255)
                                                                 aGCAGGCGCGTAATCGCACAGCAGCTCAACAACATTATAGTGCCGTGGATCCTGGCATATAGCCCGATATATCATTTTAACCTGTTCTTCCGGACCTTcc                               <  2:1951167/100‑1 (MQ=255)
                                                                 aGCAGGCGCGTAATCGCACAGCAGCTCAACAACATTATAGTGCCGTGGATCCTGGCATATAGCCCGATATATCATTTTAACCTGTTCTTCCGGACCTTcc                               <  2:1377478/100‑1 (MQ=255)
                                                                   cAGGCGCGTAATCGCACAGCAGCTCAACAACATTATAGTGCCGTGGATCCTGGCATATAGCCCGATATATCATTTTAACCTGTTCTTCCGGACCTTCCAg                             <  1:2427027/100‑1 (MQ=255)
                                                                   cAGGCGCGTAATCGCACAGCAGCTCAACAACATTATAGTGCCGTGGATCCTGGCATATAGCCCGATATATCATTTTAACCTGTTCTTCCGGACCTTCCAg                             >  2:817486/1‑100 (MQ=255)
                                                                   cAGGCGCGTAATCGCACAGCAGCTCAACAACATTATAGTGCCGTGGATCCTGGCATATAGCCCGATATATCATTTTAACCTGTTCTTCCGGACCTTCCAg                             <  1:983468/100‑1 (MQ=255)
                                                                    aGGCGCGTAATCGCACAGCAGCTCAACAACATTATAGTGCCGTGGATCCTGGCATATAGCCCGATATATCATTTTAACCTGTTCTTCCGGACCTTCCAGa                            >  1:274526/1‑100 (MQ=255)
                                                                     ggCGCGTAATCGCACAGCAGCTCAACAACATTATAGTGCCGTGGATCCTGGCATATAGCCCGATATATCATTTTAACCTGTTCTTCCGGACCTTCCAGaa                           >  2:2404567/1‑100 (MQ=255)
                                                                       cgcgTAATCGCACAGCAGCTCAACAACATTATAGTGCCGTGGATCCTGGCATATAGCCCGATATATCATTTTAACCTGTTCTTCCGGACCTTCCAGAAGc                         <  2:129795/100‑1 (MQ=255)
                                                                          gTAATCGCACAGCAGCTCAACAACATTATAGTGCCGTGGATCCTGGCATATAGCCCGATATATCATTTTAACCTGTTCTTCCGGACCTTCCAGAAGCTgg                      <  1:127639/100‑1 (MQ=255)
                                                                           tAATCGCACAGCAGCTCAACAACATTATAGTGCCGTGGATCCTGGCATATAGCCCGATATATCATTTTAACCTGTTCTTCCGGACCTTCCAGCCGCTg                       >  1:2872835/1‑98 (MQ=255)
                                                                           tAATCGCACAGCAGCTCAACAACATTATAGTGCCGTGGATCCTGGCATATAGCCCGATATATCATTTTAACCTGTTCTTCCGGACCTTCCAGAAGCTGGa                     >  1:91691/1‑100 (MQ=255)
                                                                             aTCGCACAGCAGCTCAACAACATTATAGTGCCGTGGATCCTGGCATATAGCCCGATATATCATTTTAACCTGTTCTTCCGGACCTTCCAGAAGCTGGaaa                   <  1:2077847/100‑1 (MQ=255)
                                                                              tCGCACAGCAGCTCAACAACATTATAGTGCCGTGGATCCTGGCATATAGCCCGATATATCATTTTAACCTGTTCTTCCGGACCTTCCAGAAGCTGGaaaa                  >  2:1450679/1‑100 (MQ=255)
                                                                               cGCACAGCAGCTCAACAACATTATAGTGCCGTGGATCCTGGCATATAGCCCGATATATCATTTTAACCTGTTCTTCCGGACCTTCCAGAAGCTGGaaaaa                 >  2:2580352/1‑100 (MQ=255)
                                                                               cGCACAGCAGCTCAACAACATTATAGTGCCGTGGATCCTGGCATATAGCCCGATATATCATTTTAACCTGTTCTTCCGGACCTTCCAGAAGCTGGaaaaa                 >  1:2104534/1‑100 (MQ=255)
                                                                               cGCACAGCAGCTCAACAACATTATAGTGCCGTGGATCCTGGCATATAGCCCGATATATCATTTTAACCTGTTCTTCCGGACCTTCCAGAAGCTGGaaaaa                 >  1:2105058/1‑100 (MQ=255)
                                                                               cGCACAGCAGCTCAACAACATTATAGTGCCGTGGATCCTGGCATATAGCCCGATATATCATTTTAACCTGTTCTTCCGGACCTTCCAGAAGCTGGaaaaa                 >  1:1709681/1‑100 (MQ=255)
                                                                                 cacaGCAGCTCAACAACATTATAGTGCCGTGGATCCTGGCATATAGCCCGATATATCATTTTAACCTGTTCTTCCGGACCTTCCAGAAGCTGGAAAAAAt               <  1:1414071/100‑1 (MQ=255)
                                                                                   cagcagCTCAACAACATTATAGTGCCGTGGATCCTGGCATATAGCCCGATATATCATTTTAACCTGTTCTTCCGGACCTTCCAGAAGCTGGAAAAAATga             <  1:1945883/100‑1 (MQ=255)
                                                                                   cagcagCTCAACAACATTATAGTGCCGTGGATCCTGGCATATAGCCCGATATATCATTTTAACCTGTTCTTCCGGACCTTCCAGAAGCTGGAAAAAATga             <  2:246332/100‑1 (MQ=255)
                                                                                    agcagcTCAACAACATTATAGTGCCGTGGATCCTGGCATCTAGCCCGATATATCATTTTAACCTGTTCTTCCGGACCTTCCACAAGCTGGaaacaa                >  1:1966110/1‑96 (MQ=255)
                                                                                    agcagcTCAACAACATTATAGTGCCGTGGATCCTGGCATATAGCCCGATATATCATTTTAACCTGTTCTTCCGGACCTTCCAGAAGCTGGAAAAAATgag            <  2:25684/100‑1 (MQ=255)
                                                                                    agcagcTCAACAACATTATAGTGCCGTGGATCCTGGCATATAGCCCGATATATCATTTTAACCTGTTCTTCCGGACCTTCCAGAAGCTGGAAAAAATgag            <  1:2702364/100‑1 (MQ=255)
                                                                                      cagcTCAACAACATTATAGTGCCGTGGATCCTGGCATATAGCCCGATATATCATTTTAACCTGTTCTTCCGGACCTTCCAGAAGCTGGAAAAAATGAGaa          >  1:2459172/1‑100 (MQ=255)
                                                                                       agcTCAACAACATTATAGTGCCGTGGATCCTGGCATATAGCCCGATATATCATTTTAACCTGTTCTTCCGGACCTTCCAGAAGCTGGAAAAAATGAGAAc         >  1:277066/1‑100 (MQ=255)
                                                                                        gcTCAACAACATTATAGTGCCGTGGATCCTGGCATATAGCCCGATATATCATTTTAACCTGTTCTTCCGGACCTTCCAGAAGCTGGAAAAAATGAGAAcc        >  2:1123740/1‑100 (MQ=255)
                                                                                        gcTCAACAACATTATAGTGCCGTGGATCCTGGCATATAGCCCGATATATCATTTTAACCTGTTCTTCCGGACCTTCCAGAAGCTGGAAAAAATGAGAAcc        >  1:1597979/1‑100 (MQ=255)
                                                                                        gcTCAACAACATTATAGTGCCGTGGATCCTGGCATATAGCCCGATATATCATTTTAACCTGTTCTTCCGGACCTTCCAGAAGCTGGAAAAAATGAGAAcc        <  1:117693/100‑1 (MQ=255)
                                                                                          tCAACAACATTATAGTGCCGTGGATCCTGGCATATAGCCCGATATATCATTTTAACCTGTTCTTCCGGACCTTCCAGAAGCTGGAAAAAATGAGAACCAt      >  1:789279/1‑100 (MQ=255)
                                                                                          tCAACAACATTATAGTGCCGTGGATCCTGGCATATAGCCCGATATATCATTTTAACCTGTTCTTCCGGACCTTCCAGAAGCTGGAAAAAATGAGAACCAt      >  1:301043/1‑100 (MQ=255)
                                                                                           caacaaTATTATAGTGCCGTGGATCCTGGCATATAGCCCGATATATCATTTTAACCTGTTCTTCCGGACCTTCCAGAAGCTGGAAAAAATGAGAACCAtt     >  1:2581419/1‑100 (MQ=255)
                                                                                           caacaaCATTATAGTGCCGTGGATCCTGGCATATAGCCCGATATATCATTTTAACCTGTTCTTCCGGACCTTCCAGAAGCTGGAAAAAATGAGAACCAtt     >  2:2049054/1‑100 (MQ=255)
                                                                                           caacaaCATTATAGTGCCGTGGATCCTGGCATATAGCCCGATATATCATTTTAACCTGTTCTTCCGGACCTTCCAGAAGCTGGAAAAAATGAGAACCAtt     >  1:1756563/1‑100 (MQ=255)
                                                                                           caacaaCATTATAGTGCCGTGGATCCTGGCATATAGCCCGATATATCATTTTAACCTGTTCTTCCGGACCTTCCAGAAGCTGGAAAAAATGAGAACCAtt     >  1:2543041/1‑100 (MQ=255)
                                                                                           caacaaCATTATAGTGCCGTGGATCCTGGCATATAGCCCGATATATCATTTTAACCTGTTCTTCCGGACCTTCCAGAAGCTGGAAAAAATGAGAACCAtt     >  1:512855/1‑100 (MQ=255)
                                                                                           caacaaCATTATAGTGCCGTGGATCCTGGCATATAGCCCGATATATCATTTTAACCTGTTCTTCCGGACCTTCCAGAAGCTGGAAAAAATGAGAACCAtt     <  1:817484/100‑1 (MQ=255)
                                                                                            aacaacATTATAGTGCCGTGGATCCTGGCATATAGCCCGATATATCATTTTAACCTGTTCTTCCGGACCTTCCAGAAGCTGGAAAAAATGAGAACCATTa    <  1:749918/100‑1 (MQ=255)
                                                                                            aacaacATTATAGTGCCGTGGATCCTGGCATATAGCCCGATATATCATTTTAACCTGTTCTTCCGGACCTTCCAGAAGCTGGAAAAAATGAGAACCATTa    >  1:987294/1‑100 (MQ=255)
                                                                                            aacaacATTATAGTGCCGTGGATCCTGGCATATAGCCCGATATATCATTTTAACCTGTTCTTCCGGACCTTCCAGAAGCTGGAAAAAATGAGAACCATTa    <  2:2374146/100‑1 (MQ=255)
                                                                                             acaacaTTATAGTGCCGTGGATCCTGGCATATAGCCCGATATATCATTTTAACCTGTTCTTCCGGACCTTCCAGAAGCTGGAAAAAATGAGAACCATTaa   >  2:799250/1‑100 (MQ=255)
                                                                                             acaacaTTATAGTGCCGTGGATCCTGGCATATAGCCCGATATATCATTTTAACCTGTTCTTCCGGACCTTCCAGAAGCTGGAAAAAATGAGAACCATTaa   >  1:1225867/1‑100 (MQ=255)
                                                                                              caaCATTATAGTGCCGTGGATCCTGGCATATAGCCCGATATATCATTTTAACCTGTTCTTCCGGACCTTCCAGAAGCTGGAAAAAATGAGAACCATTaaa  >  1:2369174/1‑100 (MQ=255)
                                                                                              caaCATTATAGTGCCGTGGATCCTGGCATATAGCCCGATATATCATTTTAACCTGTTCTTCCGGACCTTCCAGAAGCTGGAAAAAATGAGAACCATTaaa  >  1:1250141/1‑100 (MQ=255)
                                                                                                 |                                                                                                
AAAACGTCATCTCGCTCGTGCAGGCGCAAATCAAATAATTCCATTCCCGCTTTGCCAAAACGGCGAGCAGGCGCGTAATCGCACAGCAGCTCAACAATATTATAGTGCCGTGGATCCTGGCATATAGCCCGATATATCATTTTAACCTGTTCTTCCGGACCTTCCAGAAGCTGGAAAAAATGAGAACCATTAAA  >  NC_000913/1215159‑1215352

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: