Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,338,928 C→A 100% L107F (TTG→TTT)  mlaE ← ABC transporter maintaining OM lipid asymmetry, inner membrane permease protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,338,9280CA98.4% 408.6 / ‑2.4 121L107F (TTG→TTT) mlaEABC transporter maintaining OM lipid asymmetry, inner membrane permease protein
Reads supporting (aligned to +/- strand):  ref base C (0/2);  new base A (46/73);  total (46/75)
Fisher's exact test for biased strand distribution p-value = 5.25e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.67e-01

Only 100 of 132 total aligned reads displayed.

GCGGATCCACCGCCATCATCTCCATACTGGAGAGTTGCTCTGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAACAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATACCCAGACTGGTTTCCGCACTATAAGTGGTCAGAACCA  >  NC_000913/3338831‑3339022
                                                                                                 |                                                                                              
gCGGATCCACCGCCATCATCTCCATACTGGAGAGTTGCTCTGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCaaaaaa                                                                                              <  1:2617038/100‑1 (MQ=255)
 cGGATCCACCGCCATCATCTCCATACTGGAGAGTTGCTCTGTATCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAaaaaac                                                                                             <  2:1437227/100‑1 (MQ=255)
 cGGATCCACCGCCATCATCTCCATACTGGAGAGTTGCTCTGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAaaaaac                                                                                             <  1:2874970/100‑1 (MQ=255)
  ggATCCACCGCCATCATCTCCATAGTGGAGAGTTGCTCTGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAaaaaaca                                                                                            >  1:2670512/1‑100 (MQ=255)
  ggATCCACCGCCATCATCTCCATACTGGAGAGTTGCTCTGTAGCGCGCATCACGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAaaaaac                                                                                             <  1:1371208/99‑1 (MQ=255)
   gATCCACCGCCATCATCTCCATACTGGAGAGTTGCTCTGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAaaaaacaa                                                                                           >  2:2994341/1‑100 (MQ=255)
    aTCCACCGCCATCATCTCCATACTGGAGAGTTGCTCTGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAaaaaacaac                                                                                          <  1:466804/100‑1 (MQ=255)
    aTCCACCGCCATCATCTCCATACTGGAGAGTTGCTCTGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAaaaaacaac                                                                                          <  2:1101858/100‑1 (MQ=255)
    aTCCACCGCCATCATCTCCATACTGGAGAGTTGCTCTGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAaaaaacaac                                                                                          <  2:1527046/100‑1 (MQ=255)
     tCCACCGCCATCATCTCCATACTGGAGAGTTGCTCTGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAAcg                                                                                         >  1:1028216/1‑100 (MQ=255)
      ccACCGCCATCATCTCCATACTGGAGAGTTGCTCTGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAAcgc                                                                                        <  2:2535904/100‑1 (MQ=255)
       cACCGCCATCATCTCCATACTGGAGAGTTGCTCTGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAAcgcg                                                                                       >  1:151244/1‑100 (MQ=255)
       cACCGCCATCATCTCCATACTGGAGAGTTGCTCTGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAAcgcg                                                                                       >  2:2009179/1‑100 (MQ=255)
       cACCGCCATCATCTCCATACTGGAGAGTTGCTCTGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAAcgcg                                                                                       >  1:2991840/1‑100 (MQ=255)
        aCCGCCATCATCTCCATACTGGAGAGTTGCTCTGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCgg                                                                                      <  2:2212453/100‑1 (MQ=255)
         ccGCCATCATCTCCATACTGGAGAGTTGCTCTGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGc                                                                                     <  2:3235298/100‑1 (MQ=255)
           gCCATCATCTCCATACTGGAGAGTTGCTCTGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCaa                                                                                   >  2:3285400/1‑100 (MQ=255)
           gCCATCATCTCCATACTGGAGAGTTGCTCTGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCaa                                                                                   >  1:2069380/1‑100 (MQ=255)
            ccATCATCTCCATACTGGAGAGTTGCTCTGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAac                                                                                  <  1:46176/100‑1 (MQ=255)
             catcatCTCCATACTGGAGAGTTGCTGTGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAacc                                                                                 <  1:2344899/100‑1 (MQ=255)
                catcTCCATACTGGAGAGTTGCTCTGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAaccacc                                                                              <  2:894140/100‑1 (MQ=255)
                catcTCCATACTGGAGAGTTGCTCTGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAaccacc                                                                              >  1:599858/1‑100 (MQ=255)
                 atcTCCATACTGGAGAGTTGCTCTGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCg                                                                             >  1:3207625/1‑100 (MQ=255)
                  tctcCATACTGGAGAGTTGCTCTGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCgg                                                                            >  2:1974964/1‑100 (MQ=255)
                  tctcCATACTGGAGAGTTGCTCTGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCgg                                                                            <  2:3235154/100‑1 (MQ=255)
                   ctcCATACCGGAGAGTTGCTCCGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGc                                                                           <  1:738704/100‑1 (MQ=255)
                    tcCATACTGGAGAGTTGCTCTGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGcc                                                                          >  2:2223545/1‑100 (MQ=255)
                     ccATACTGGAGAGTTGCTCTGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAACAACAACGCGGCAACCACCGGcc                                                                          <  2:596760/99‑1 (MQ=255)
                     ccATACTGGAGAGTTGCTCTGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGccc                                                                         >  2:742193/1‑100 (MQ=255)
                        tACTGGAGAGTTGCTCTGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAg                                                                      >  1:2954008/1‑100 (MQ=255)
                        tACTCGAGAGTTGCTCTGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAg                                                                      <  1:2922612/100‑1 (MQ=255)
                         aCTGGAGAGTTGCTCTGTAGCGCGCATCAGGCTGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGt                                                                     <  1:1033443/100‑1 (MQ=255)
                         aCTGGAGAGTTGCTCTGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGt                                                                     <  2:1539583/100‑1 (MQ=255)
                         aCTGGAGAGTTGCTCTGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGt                                                                     <  1:377269/100‑1 (MQ=255)
                         aCTGGAGAGTTGCTCGGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAg                                                                      <  1:3133032/99‑1 (MQ=255)
                          cTGGAGAGTTGCTCTGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGtt                                                                    <  1:2332266/100‑1 (MQ=255)
                          cTGGAGAGTTGCTCTGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGtt                                                                    >  2:407416/1‑100 (MQ=255)
                          cTGGAGAGTTGCTCTGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGtt                                                                    >  2:26080/1‑100 (MQ=255)
                            ggtgagTTGCTCTTTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGTCAAAAAACAACGCGGCAACCACCGGCCCCAGtt                                                                    <  1:2332394/95‑1 (MQ=255)
                            ggAGAGTTGCTCTGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCa                                                                  >  1:1142770/1‑100 (MQ=255)
                             gagaGTTGCTCTGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCCGTTCAc                                                                 >  1:2177200/1‑100 (MQ=255)
                               gagTTGCTCTGTAACGAGAATCAGGCCGATTTCTGAGGTTAGCGCAGAACCAGCACGCCCGGCAAAAAACAACGCGGCACCCTCCGGCCACAGTTCACGc                                                               <  1:2048872/100‑1 (MQ=255)
                                agTTGCTCTGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCa                                                              >  1:510775/1‑100 (MQ=255)
                                  ttGCTCTGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGt                                                            <  2:83747/100‑1 (MQ=255)
                                      tctGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCg                                                        <  1:2434859/100‑1 (MQ=255)
                                      tctGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCg                                                        <  1:651713/100‑1 (MQ=255)
                                        tGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGAt                                                      <  2:295907/100‑1 (MQ=255)
                                         gTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATa                                                     <  1:894364/100‑1 (MQ=255)
                                         gTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATa                                                     <  1:1651524/100‑1 (MQ=255)
                                         gTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCAAGGAAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATa                                                     <  1:388170/100‑1 (MQ=255)
                                         gTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGAACACAAAACAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATa                                                     <  1:2809292/100‑1 (MQ=255)
                                           aGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGTACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGc                                                         <  1:774799/94‑1 (MQ=255)
                                           aGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAAc                                                   >  1:1172857/1‑100 (MQ=255)
                                           aGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAAc                                                   >  2:758535/1‑100 (MQ=255)
                                           aGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAAc                                                   >  1:3263722/1‑100 (MQ=255)
                                           aGCGCGCATCAGGCCGATTTCTGCGGTGAGCGCCGAACCAGCACTCCCGGCAAAAAACAACGCTGCAACCACCGGCCCCAGTTCACGCAGTAGCGATa                                                     >  1:1953145/1‑98 (MQ=255)
                                            gcgcgcATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGACCGACAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACg                                                  <  1:2223528/100‑1 (MQ=255)
                                            gcgcgcATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACACCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACg                                                  >  1:886077/1‑100 (MQ=255)
                                              gcgcATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGcc                                                >  2:1230457/1‑100 (MQ=255)
                                                 cATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGccacc                                             >  1:1622636/1‑100 (MQ=255)
                                                 cATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGccac                                              >  1:1252457/1‑99 (MQ=255)
                                                  aTCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGccacca                                            <  2:1207769/100‑1 (MQ=255)
                                                  aTCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGccacca                                            <  2:2612228/100‑1 (MQ=255)
                                                   tCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAg                                           <  2:403250/100‑1 (MQ=255)
                                                    cAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGc                                          >  1:1433038/1‑100 (MQ=255)
                                                    cAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGc                                          <  1:1688259/100‑1 (MQ=255)
                                                         cGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATAcc                                     <  2:3321927/100‑1 (MQ=255)
                                                           aTTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATACCCa                                   <  1:2422756/100‑1 (MQ=255)
                                                           aTTTCGGCGGTTAGGGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATAc                                      <  1:965420/97‑1 (MQ=255)
                                                               cTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATACCCAGACt                               >  1:2708223/1‑100 (MQ=255)
                                                                 gCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATACCCAGACTgg                             <  2:832982/100‑1 (MQ=255)
                                                                 gCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATACCCAGACTgg                             <  1:832160/100‑1 (MQ=255)
                                                                   ggTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATACCCAGACTGGtt                           >  2:1787296/1‑100 (MQ=255)
                                                                    gTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATACCCAGACTGGttt                          <  2:57750/100‑1 (MQ=255)
                                                                     ttAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATACCCAGACTGGTTTc                         <  2:510775/100‑1 (MQ=255)
                                                                       aGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATACCCAGACTGGTTTCCg                       <  2:2362423/100‑1 (MQ=255)
                                                                        gcgcCGATCCAGCCCGACCGACAAAAAACAACTCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATACCCAGACTGGTTTCCGc                      <  1:1531812/100‑1 (MQ=255)
                                                                        gcgcCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATACCCAGACTGGTTTCCGc                      <  2:2366297/100‑1 (MQ=255)
                                                                        gcgcCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATACCCAGACTGGTTTCCGc                      <  2:2883295/100‑1 (MQ=255)
                                                                          gcCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATACCCAGACTGGTTTCCGCAc                    <  2:2305179/100‑1 (MQ=255)
                                                                          gcCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATACCCAGACTGGTTTCCGCAc                    <  2:1501241/100‑1 (MQ=255)
                                                                          gcCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATACCCAGACTGGTTTCCGCAc                    <  1:2166727/100‑1 (MQ=255)
                                                                              aaCCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATACCCAGACTGGTTTCCGCACtata                <  1:2419255/100‑1 (MQ=255)
                                                                                ccAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATACCCAGACTGGTTTCCGCACTATAAg              >  2:1515081/1‑100 (MQ=255)
                                                                                ccAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATACCCAGACTGGTTTCCGCACTATAAg              >  1:807783/1‑100 (MQ=255)
                                                                                 cAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATACCCAGACTGGTTTCCGCACTATAAGt             <  1:3317251/100‑1 (MQ=255)
                                                                                 cAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATACCCAGACTGGTTTCCGCACTATAAGt             <  2:2491223/100‑1 (MQ=255)
                                                                                 cAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATACCCAGACTGGTTTCCGCACTATAAGt             <  1:595543/100‑1 (MQ=255)
                                                                                  aGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATACCCAGACTGGTTTCCGCACTATAAGTg            >  2:3121469/1‑100 (MQ=255)
                                                                                   acACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATACCCAGACTGGTTTCCGCACTATAAGTgg           <  1:875663/99‑1 (MQ=255)
                                                                                    cACGCCGGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATACCCAGACTGGTTTCCGCACTATAAGTgg           <  1:1342779/99‑1 (MQ=255)
                                                                                    cACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAATGCCACCAGCATACCCAGACTGGTTTCCGCACTATAAGTGGt          <  2:2357100/100‑1 (MQ=255)
                                                                                    cACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATACCCAGACTGGTTTCCGCACTATAAGTgg           >  1:3281470/1‑99 (MQ=255)
                                                                                      cGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATACCCAGACTGGTTTCCGCACTATAAGTGGTCa        >  2:1507066/1‑100 (MQ=255)
                                                                                       gCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATACCCAGACTGGTTTCCGCACTATAAGTGGTCAg       >  2:148903/1‑100 (MQ=255)
                                                                                         ccGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATACCCAGACTGGTTTCCGCACTATAAGTGGTCAGaa     >  2:295621/1‑100 (MQ=255)
                                                                                           ggCAAACAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATACCCAGACTGGTTTCCGCACTATAAGTGGTCAGAAcc   <  2:676747/100‑1 (MQ=255)
                                                                                           ggCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATACCCAGACTGGTTTCCGCACTATAAGTGGTCAGAAcc   <  2:3084658/100‑1 (MQ=255)
                                                                                           ggCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATACCCAGACTGGTTTCCGCACTATAAGTGGTCAGAAcc   <  1:26079/100‑1 (MQ=255)
                                                                                            gCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATACCCAGACTGGTTTCCGCACTATAAGTGGTCAGAACCa  <  2:1142509/100‑1 (MQ=255)
                                                                                                 |                                                                                              
GCGGATCCACCGCCATCATCTCCATACTGGAGAGTTGCTCTGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAACAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATACCCAGACTGGTTTCCGCACTATAAGTGGTCAGAACCA  >  NC_000913/3338831‑3339022

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑