Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC JC	NC_000913	3,410,998	IS2 (+) +5 bp	coding (719‑723/966 nt)	dusB →	tRNA‑dihydrouridine synthase B

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	3186096 =	NA (NA)	83 (1.220)	48/198	0.1	100%	noncoding (1/1331 nt)	IS2	repeat region
	?	NC_000913	= 3411002	0 (0.000)					coding (723/966 nt)	dusB	tRNA‑dihydrouridine synthase B
*	?	NC_000913	= 3187426	NA (NA)	71 (1.050)	39/198	0.4	100%	noncoding (1331/1331 nt)	IS2	repeat region
	?	NC_000913	3410998 =	0 (0.000)					coding (719/966 nt)	dusB	tRNA‑dihydrouridine synthase B

TAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCA‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913/3187328‑3187426 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑ATCTGGACACTGGGGAGTTGCTGCCCCCGCTGCCTTTGGCAGAGGTTAAGCGCTTGCTTTGCGCGCACGTTCGGGAACTGCATGACTTTTATGGTCCG  >  NC_000913/3410998‑3411095                                                                                                                                                                                                       TAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAA                                                                                                   >  2:759868/1‑100 TAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAA                                                                                                   >  1:759868/1‑100  AGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAAT                                                                                                  <  2:743074/100‑1  AGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAAT                                                                                                  <  2:533018/100‑1  AGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAAT                                                                                                  <  1:533018/100‑1  AGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAAT                                                                                                  <  1:743074/100‑1    TGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAATCT                                                                                                >  2:1701792/1‑100        CTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAATCTGGAC                                                                                            >  1:482758/1‑100        CTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAATCTGGAC                                                                                            >  2:482758/1‑100               ATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAATCTGGACACTGGGG                                                                                     <  1:454821/100‑1               ATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAATCTGGACACTGGGG                                                                                     <  2:454821/100‑1                  GCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAATCTGGACACTGGGGAGT                                                                                  <  2:924268/100‑1                  GCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAATCTGGACACTGGGGAGT                                                                                  >  2:1481138/1‑100                      GCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAATCTGGACACTGGGGAGTTGCT                                                                              <  2:2557108/100‑1                       CCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAATCTGGACACTGGGGAGTTGCTG                                                                             <  2:2359189/100‑1                       CCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAATCTGGACACTGGGGAGTTGCTG                                                                             <  2:227422/100‑1                       CCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAATCTGGACACTGGGGAGTTGCTG                                                                             <  1:227422/100‑1                         ACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAATCTGGACACTGGGGAGTTGCTGCC                                                                           >  2:2514995/1‑100                         ACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAATCTGGACACTGGGGAGTTGCTGCC                                                                           <  2:1042422/100‑1                           GGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAATCTGGACACTGGGGAGTTGCTGCCCC                                                                         <  1:144381/100‑1                           GGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAATCTGGACACTGGGGAGTTGCTGCCCC                                                                         <  2:144381/100‑1                            GGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAATCTGGACACTGGGGAGTTGCTGCCCCC                                                                        >  2:2329069/1‑100                               ATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAATCTGGACACTGGGGAGTTGCTGCCCCCGCT                                                                     <  1:746605/100‑1                               ATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAATCTGGACACTGGGGAGTTGCTGCCCCCGCT                                                                     <  2:746605/100‑1                                 ATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAATCTGGACACTGGGGAGTTGCTGCCCCCGCTGC                                                                   >  2:118329/1‑100                                 ATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAATCTGGACACTGGGGAGTTGCTGCCCCCGCTGC                                                                   >  1:118329/1‑100                                  TCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAATCTGGACACTGGGGAGTTGCTGCCCCCGCTGCC                                                                  >  2:2427035/1‑100                                      CGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAATCTGGACACTGGGGAGTTGCTGCCCCCGCTGCCTTTG                                                              <  2:2113716/100‑1                                        GCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAATCTGGACACTGGGGAGTTGCTGCCCCCGCTGCCTTTGGC                                                            <  2:1185008/100‑1                                        GCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAATCTGGACACTGGGGAGTTGCTGCCCCCGCTGCCTTTGGC                                                            <  1:193552/100‑1                                        GCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAATCTGGACACTGGGGAGTTGCTGCCCCCGCTGCCTTTGGC                                                            <  2:193552/100‑1                                         CAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAACCCAATCTGGACACTGGGGAGTTGCTGCCCCCGCTGCCTTTGGCA                                                           <  2:1947283/100‑1                                           GCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAATCTGGACACTGGGGAGTTGCTGCCCCCGCTGCCTTTGGCAGA                                                         <  2:1776953/100‑1                                              GGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAATCTGGACACTGGGGAGTTGCTGCCCCCGCTGCCTTTGGCAGAGGT                                                      <  2:690311/100‑1                                              GGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAATCTGGACACTGGGGAGTTGCTGCCCCCGCTGCCTTTGGCAGAGGT                                                      <  1:690311/100‑1                                              GGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAATCTGGACACTGGGGAGTTGCTGCCCCCGCTGCCTTTGGCAGAGGT                                                      <  2:1440287/100‑1                                                CTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAATCTGGACACTGGGGAGTTGCTGCCCCCGCTGCCTTTGGCAGAGGTTA                                                    >  2:1014004/1‑100                                                         GGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAATCTGGACACTGGGGAGTTGCTGCCCCCGCTGCCTTTGGCAGAGGTTAAGCGCTTGC                                           <  1:144766/100‑1                                                         GGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAATCTGGACACTGGGGAGTTGCTGCCCCCGCTGCCTTTGGCAGAGGTTAAGCGCTTGC                                           <  2:144766/100‑1                                                         GGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAATCTGGACACTGGGGAGTTGCTGCCCCCGCTGCCTTTGGCAGAGGTTAAGCGCTTGC                                           >  2:1949125/1‑100                                                             AAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAATCTGGACACTGGGGAGTTGCTGCCCCCGCTGCCTTTGGCAGAGGTTAAGCGCTTGCTTTG                                       <  2:1899437/100‑1                                                             AAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAATCTGGACACTGGGGAGTTGCTGCCCCCGCTGCCTTTGGCAGAGGTTAAGCGCTTGCTTTG                                       >  2:2199555/1‑100                                                              AGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAATCTGGACACTGGGGAGTTGCTGCCCCCGCTGCCTTTGGCAGAGGTTAAGCGCTTGCTTTGC                                      >  1:706570/1‑100                                                              AGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAATCTGGACACTGGGGAGTTGCTGCCCCCGCTGCCTTTGGCAGAGGTTAAGCGCTTGCTTTGC                                      >  2:706570/1‑100                                                               GTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAATCTGGACACTGGGGAGTTGCTGCCCCCGCTGCCTTTGGCAGAGGTTAAGCGCTTGCTTTGCG                                     <  2:1661694/100‑1                                                                   TAACAGATGTCTGGAAATATAGGGGCAAATCCAATCTGGACACTGGGGAGTTGCTGCCCCCGCTGCCTTTGGCAGAGGTTAAGCGCTTGCTTTGCGCGCA                                 <  1:756929/100‑1                                                                   TAACAGATGTCTGGAAATATAGGGGCAAATCCAATCTGGACACTGGGGAGTTGCTGCCCCCGCTGCCTTTGGCAGAGGTTAAGCGCTTGCTTTGCGCGCA                                 <  2:756929/100‑1                                                                        GATGTCTGGAAATATAGGGGCAAATCCAATCTGGACACTGGGGAGTTGCTGCCCCCGCTGCCTTTGGCAGAGGTTAAGCGCTTGCTTTGCGCGCACGTTC                            >  1:795430/1‑100                                                                        GATGTCTGGAAATATAGGGGCAAATCCAATCTGGACACTGGGGAGTTGCTGCCCCCGCTGCCTTTGGCAGAGGTTAAGCGCTTGCTTTGCGCGCACGTTC                            >  2:795430/1‑100                                                                          TGTCTGGAAATATAGGGGCAAATCCAATCTGGACACTGGGGAGTTGCTGCCCCCGCTGCCTTTGGCAGAGGTTAAGCGCTTGCTTTGCGCGCACGTTCGG                          <  1:4335/100‑1                                                                          TGTCTGGAAATATAGGGGCAAATCCAATCTGGACACTGGGGAGTTGCTGCCCCCGCTGCCTTTGGCAGAGGTTAAGCGCTTGCTTTGCGCGCACGTTCGG                          <  2:4335/100‑1                                                                           GTCTGGAAATATAGGGGCAAATCCAATCTGGACACTGGGGAGTTGCTGCCCCCGCTGCCTTTGGCAGAGGTTAAGCGCTTGCTTTGCGCGCACGTTCGGG                         <  2:5157/100‑1                                                                           GTCTGGAAATATAGGGGCAAATCCAATCTGGACACTGGGGAGTTGCTGCCCCCGCTGCCTTTGGCAGAGGTTAAGCGCTTGCTTTGCGCGCACGTTCGGG                         <  1:5157/100‑1                                                                             CTGGAAATATAGGGGCAAATCCAATCTGGACACTGGGGAGTTGCTGCCCCCGCTGCCTTTGGCAGAGGTTAAGCGCTTGCTTTGCGCGCACGTTCGGGAA                       >  2:1817317/1‑100                                                                                GAAATATAGGGGCAAATCCAATCTGGACACTGGGGAGTTGCTGCCCCCGCTGCCTTTGGCAGAGGTTAAGCGCTTGCTTTGCGCGCACGTTCGGGAACTG                    <  2:192036/100‑1                                                                                GAAATATAGGGGCAAATCCAATCTGGACACTGGGGAGTTGCTGCCCCCGCTGCCTTTGGCAGAGGTTAAGCGCTTGCTTTGCGCGCACGTTCGGGAACTG                    <  1:192036/100‑1                                                                                 AAATATAGGGGCAAATCCAATCTGGACACTGGGGAGTTGCTGCCCCCGCTGCCTTTGGCAGAGGTTAAGCGCTTGCTTTGCGCGCACGTTCGGGAACTGC                   >  2:1507142/1‑100                                                                                  AATATAGGGGCAAATCCAATCTGGACACTGGGGAGTTGCTGCCCCCGCTGCCTTTGGCAGAGGTTAAGCGCTTGCTTTGCGCGCACGTTCGGGAACTGCn                  <  2:1126811/100‑2                                                                                  AATATAGGGGCAAATCCAATCTGGACACTGGGGAGTTGCTGCCCCCGCTGCCTTTGGCAGAGGTTAAGCGCTTGCTTTGCGCGCACGTTCGGGAACTGCA                  <  2:1837893/100‑1                                                                                    TATAGGGGCAAATCCAATCTGGACACTGGGGAGTTGCTGCCCCCGCTGCCTTTGGCAGAGGTTAAGCGCTTGCTTTGCGCGCACGTTCGGGAACTGCATG                <  2:1690110/100‑1                                                                                          GGCAAATCCAATCTGGACACTGGGGAGTTGCTGCCCCCGCTGCCTTTGGCAGAGGTTAAGCGCTTGCTTTGCGCGCACGTTCGGGAACTGCATGACTTTT          >  2:600190/1‑100                                                                                          GGCAAATCCAATCTGGACACTGGGGAGTTGCTGCCCCCGCTGCCTTTGGCAGAGGTTAAGCGCTTGCTTTGCGCGCACGTTCGGGAACTGCATGACTTTT          >  1:600190/1‑100                                                                                          GGCAAATCCAATCTGGACACTGGGGAGTTGCTGCCCCCGCTGCCTTTGGCAGAGGTTAAGCGCTTGCTTTGCGCGCACGTTCGGGAACTGCATGACTTTT          >  1:718481/1‑100                                                                                          GGCAAATCCAATCTGGACACTGGGGAGTTGCTGCCCCCGCTGCCTTTGGCAGAGGTTAAGCGCTTGCTTTGCGCGCACGTTCGGGAACTGCATGACTTTT          >  2:718481/1‑100                                                                                          GGCAAATCCAATCTGGACACTGGGGAGTTGCTGCCCCCGCTGCCTTTGGCAGAGGTTAAGCGCTTGCTTTGCGCGCACGTTCGGGAACTGCATGACTTTT          >  2:1229691/1‑100                                                                                          GGCAAATCCAATCTGGACACTGGGGAGTTGCTGCCCCCGCTGCCTTTGGCAGAGGTTAAGCGCTTGCTTTGCGCGCACGTTCGGGAACTGCATGACTTTT          >  2:1014080/1‑100                                                                                              AATCCAATCTGGACACTGGGGAGTTGCTGCCCCCGCTGCCTTTGGCAGAGGTTAAGCGCTTGCTTTGCGCGCACGTTCGGGAACTGCATGACTTTTATGG      <  1:568863/100‑1                                                                                              AATCCAATCTGGACACTGGGGAGTTGCTGCCCCCGCTGCCTTTGGCAGAGGTTAAGCGCTTGCTTTGCGCGCACGTTCGGGAACTGCATGACTTTTATGG      <  2:568863/100‑1                                                                                                 CCAATCTGGACACTGGGGAGTTGCTGCCCCCGCTGCCTTTGGCAGAGGTTAAGCGCTTGCTTTGCGCGCACGTTCGGGAACTGCATGACTTTTATGGTCC   >  2:507620/1‑100                                                                                                 CCAATCTGGACACTGGGGAGTTGCTGCCCCCGCTGCCTTTGGCAGAGGTTAAGCGCTTGCTTTGCGCGCACGTTCGGGAACTGCATGACTTTTATGGTCC   >  1:507620/1‑100                                                                                                  CAATCTGGACACTGGGGAGTTGCTGCCCCCGCTGCCTTTGGCAGAGGTTAAGCGCTTGCTTTGCGCGCACGTTCGGGAACTGCATGACTTTTATGGTCCG  >  2:2209148/1‑100                                                                                                                                                                                                       TAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCA‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913/3187328‑3187426 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑ATCTGGACACTGGGGAGTTGCTGCCCCCGCTGCCTTTGGCAGAGGTTAAGCGCTTGCTTTGCGCGCACGTTCGGGAACTGCATGACTTTTATGGTCCG  >  NC_000913/3410998‑3411095

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.