| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 756,202 | T→G | L99R (CTC→CGC) | sdhA → | succinate dehydrogenase, flavoprotein subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 756,202 | 0 | T | G | 98.2% | 207.3 / ‑4.4 | 56 | L99R (CTC→CGC) | sdhA | succinate dehydrogenase, flavoprotein subunit |
| Reads supporting (aligned to +/- strand): ref base T (0/1); new base G (22/33); total (22/34) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.94e-01 | |||||||||||
TGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGAAAAACTTCGGCGGCGAGC > NC_000913/756105‑756294 | gatcttatGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAAcgcg < 2:1495943/95‑1 (MQ=255) gCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGCCCAGGACGCGATTGAATATATGTGTCAAACCGGGCCGGAAGCGATTCTGGAACGCGa > 2:1000727/1‑99 (MQ=255) cATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACGCGAac < 2:418086/100‑1 (MQ=255) cATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACGCGAac < 1:418086/100‑1 (MQ=255) gTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACGCGAACACATg < 2:2184633/100‑1 (MQ=255) gTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACGCGAACACATg < 1:316115/100‑1 (MQ=255) gTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACGCGAACACATg < 2:316115/100‑1 (MQ=255) gTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACGCGAACACATg < 2:891356/100‑1 (MQ=255) aCGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATggg < 2:2210815/100‑1 (MQ=255) gACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACGCGAACACATGGGcc > 2:402670/1‑100 (MQ=255) gACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACGCGAACACATGGGcc > 1:402670/1‑100 (MQ=255) cacCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACGCGAACACATGGGCCTg > 2:2267104/1‑100 (MQ=255) acCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACGCGAACACATGGGCCTGc < 1:573258/100‑1 (MQ=255) acCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACGCGAACACATGGGCCTGc < 2:573258/100‑1 (MQ=255) aaaGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACGCGAACACATGGGCCTGCCGTtct > 2:282188/1‑100 (MQ=255) aaaGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACGCGAACACATGGGCCTGCCGTtct > 1:282188/1‑100 (MQ=255) ggTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACGCGAACACATGGGCCTGCCGTTCTcgcg < 2:1449600/100‑1 (MQ=255) gACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACGCGAACACATGGGCCTGCCGTTCTCGCGtctc < 2:851182/99‑1 (MQ=255) gACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACGCGAACACATGGGCCTGCCGTTCTCGCGTCTCg > 1:589949/1‑100 (MQ=255) gACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACGCGAACACATGGGCCTGCCGTTCTCGCGTCTCg > 2:589949/1‑100 (MQ=255) aCTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACGCGAACACATGGGCCTGCCGTTCTCGCGTCTCga < 2:618562/100‑1 (MQ=255) aCTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACGCGAACACATGGGCCTGCCGTTCTCGCGTCTCga < 1:618562/100‑1 (MQ=255) tCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACGCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATgg > 2:1876405/1‑100 (MQ=255) gTGACCAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACGCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCg < 2:716617/100‑1 (MQ=255) gTGACCAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACGCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCg < 1:716617/100‑1 (MQ=255) gTGACCAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACGCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCg < 2:1759590/100‑1 (MQ=255) ccAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACGCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGGTGGTCGtatc > 2:672949/1‑100 (MQ=255) ccAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACGCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGGTGGTCGtatc > 1:672949/1‑100 (MQ=255) cAGGACGCGATTGAATATATGTGTAAATCCGGGCCGGAAGCGATTCTGGAACGCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGtatct < 1:626981/100‑1 (MQ=255) cAGGACGCGATTGAATATATGTGTAAATCCGGGCCGGAAGCGATTCTGGAACGCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGtatct < 2:626981/100‑1 (MQ=255) gACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACGCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGtatctatc > 2:1711737/1‑100 (MQ=255) gACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACGCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGtatctatc < 2:371182/100‑1 (MQ=255) gACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACGCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGtatctatc < 1:371182/100‑1 (MQ=255) gAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACGCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGt > 2:1936368/1‑100 (MQ=255) aaTATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACGCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGtt > 2:700271/1‑100 (MQ=255) aaTATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACGCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGtt > 1:700271/1‑100 (MQ=255) atatatGTGTAAAACCGGGCCGGAAGCGATTCTGGAACGCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGttt < 2:164335/100‑1 (MQ=255) atatatGTGTAAAACCGGGCCGGAAGCGATTCTGGAACGCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGttt < 1:164335/100‑1 (MQ=255) tatatGTGTAAAACCGGGCCGGAAGCGATTCTGGAACGCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTg > 2:722936/1‑100 (MQ=255) tatatGTGTAAAACCGGGCCGGAAGCGATTCTGGAACGCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTg > 1:722936/1‑100 (MQ=255) tgtgTAAAACCGGGCCGGAAGCGATTCTGGAACGCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCgg > 2:2096337/1‑100 (MQ=255) aaaCCGGGCCGGAAGCGATTCTGGAACGCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTc < 2:344570/100‑1 (MQ=255) aaaCCGGGCCGGAAGCGATTCTGGAACGCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTc < 2:15667/100‑1 (MQ=255) aaaCCGGGCCGGAAGCGATTCTGGAACGCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTc < 1:344570/100‑1 (MQ=255) aaaCCGGGCCGGAAGCGATTCTGGAACGCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTc < 1:15667/100‑1 (MQ=255) ccGGGCCGGAAGCGATTCTGGAACGCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGaa < 2:1864476/100‑1 (MQ=255) ccGGAAGCGATTCTGGAACGCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGAAAAACt < 2:931741/100‑1 (MQ=255) gAAGCGATTCTGGAACGCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGAAAAACTTcg > 2:316234/1‑100 (MQ=255) gAAGCGATTCTGGAACGCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGAAAAACTTcg > 2:1748832/1‑100 (MQ=255) gAAGCGATTCTGGAACGCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGAAAAACTTcg < 2:1640721/100‑1 (MQ=255) gAAGCGATTCTGGAACGCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGAAAAACTTcg > 1:316234/1‑100 (MQ=255) aaGCGATTCTGGAACGCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGAAAAACTTcgg < 2:2043160/100‑1 (MQ=255) aaGCGATTCTGGAACGCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGGTTGGCGGTCAGTCGAAAAACTTcg < 2:284139/99‑1 (MQ=255) aaGCGATTCTGGAACGCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGGTTGGCGGTCAGTCGAAAAACTTcg < 1:284139/99‑1 (MQ=255) aGCGATTCTGGAACGCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGAAAAACTTcggc < 2:1200161/100‑1 (MQ=255) gATTCTGGAACGCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGAAAAACTTCggcggc > 2:1429179/1‑100 (MQ=255) ttGGAACGCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGAAAAACTTCGGCGGCGAGc < 2:2485525/99‑1 (MQ=255) | TGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGAAAAACTTCGGCGGCGAGC > NC_000913/756105‑756294 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |