Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,810,760	T→C	intergenic (+81/‑10)	ygaH → / → mprA	putative L‑valine exporter, norvaline resistance protein/transcriptional repressor of microcin B17 synthesis and multidrug efflux

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,810,760	0	T	C	100.0%	242.0 / NA	64	intergenic (+81/‑10)	ygaH/mprA	putative L‑valine exporter, norvaline resistance protein/transcriptional repressor of microcin B17 synthesis and multidrug efflux
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (38/26);  total (38/26)

GATGGCGATTATATAACCCACAAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCTTCTG  >  NC_000913/2810664‑2810856                                                                                                 |                                                                                                 gATGGCGATTATATAACCCACAAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGGCCAt                                                                                               >  1:541471/1‑100 (MQ=255) gATGGCGATTATATAACCCACAAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGGCCAt                                                                                               >  2:541471/1‑100 (MQ=255)   tGGCGATTATATAACCCACAAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGGCCATAc                                                                                             >  2:513387/1‑100 (MQ=255)   tGGCGATTATATAACCCACAAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGGCCATAc                                                                                             >  1:513387/1‑100 (MQ=255)      cGATTATATAACCCACAAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGGCCATACCCa                                                                                          <  2:1805108/100‑1 (MQ=255)          tatataACCCACAAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGGCCATACCCAAATg                                                                                      <  2:885011/100‑1 (MQ=255)             ataACCCACAAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGGCCATACCCAAATGGAt                                                                                   <  2:1573239/100‑1 (MQ=255)               aaCCCACAAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGGCCATACCCAAATGGATAg                                                                                 <  2:1034774/100‑1 (MQ=255)                aCCCACAAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGGCCATACCCAAATGGATAGt                                                                                >  2:194989/1‑100 (MQ=255)                aCCCACAAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGGCCATACCCAAATGGATAGt                                                                                >  1:194989/1‑100 (MQ=255)                   cacaAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGGCCATACCCAAATGGATAGTTct                                                                             >  2:1682677/1‑99 (MQ=255)                   cacaAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGGCCATACCCAAATGGATAGTTCg                                                                             >  1:124402/1‑100 (MQ=255)                   cacaAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGGCCATACCCAAATGGATAGTTCg                                                                             >  2:1284675/1‑100 (MQ=255)                   cacaAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGGCCATACCCAAATGGATAGTTCg                                                                             >  2:124402/1‑100 (MQ=255)                       aGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGGCCATACCCAAATGGATAGTTCGTTTa                                                                         >  2:1322159/1‑100 (MQ=255)                            cATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGGCCATACCCAAATGGATAGTTCGTTTACGccc                                                                    >  2:893731/1‑100 (MQ=255)                                tttCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGGCCATACCCAAATGGATAGTTCGTTTACGCCCATTg                                                                >  1:239020/1‑100 (MQ=255)                                tttCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGGCCATACCCAAATGGATAGTTCGTTTACGCCCATTg                                                                >  2:239020/1‑100 (MQ=255)                                tttCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGGCCATACCCAAATGGATAGTTCGTTTACGCCCATTg                                                                <  1:228095/100‑1 (MQ=255)                                tttCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGGCCATACCCAAATGGATAGTTCGTTTACGCCCATTg                                                                <  2:228095/100‑1 (MQ=255)                                tttCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGGCCATACCCAAATGGATAGTTCGTTTACGCCCATTg                                                                >  2:1579475/1‑100 (MQ=255)                                   cTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGGCCATACCCAAATGGATAGTTCGTTTACGCCCATTGAAc                                                             <  2:261438/100‑1 (MQ=255)                                   cTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGGCCATACCCAAATGGATAGTTCGTTTACGCCCATTGAAc                                                             <  1:261438/100‑1 (MQ=255)                                    tAAAACAATACATTTACTTTATTTGTCACTGTCGTTGCTATATCGGCTGAAATTAATGAGGCCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACa                                                            >  2:1140283/1‑100 (MQ=255)                                     aaaaCAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGGCCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACaa                                                           >  2:1292796/1‑100 (MQ=255)                                        aCAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGGCCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATg                                                        <  2:1622551/100‑1 (MQ=255)                                            tACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGGCCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTaa                                                    >  2:1929009/1‑100 (MQ=255)                                                tttACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGGCCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAAtt                                                <  2:1864680/100‑1 (MQ=255)                                                 ttACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGGCCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAAttt                                               <  2:1745443/100‑1 (MQ=255)                                                   aCTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGGCCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTcg                                             >  2:1539882/1‑100 (MQ=255)                                                   aCTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGGCCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTcg                                             <  2:1595923/100‑1 (MQ=255)                                                    cTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGGCCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTcgc                                            <  2:1868704/100‑1 (MQ=255)                                                       tATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGGCCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGcc                                         <  2:228727/100‑1 (MQ=255)                                                       tATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGGCCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGcc                                         <  1:228727/100‑1 (MQ=255)                                                              cACTGTCGTTACTATATCGGCTGAAATTAATGAGGCCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAgccgcc                                  >  2:1539039/1‑100 (MQ=255)                                                              cACTGTCGTTACTATATCGGCTGAAATTAATGAGGCCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAgccgcc                                  >  2:1632580/1‑100 (MQ=255)                                                                cTGTCGTTACTATATCGGCTGAAATTAATGAGGCCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCAc                                <  2:954372/100‑1 (MQ=255)                                                                 tGTCGTTACTATATCGGCTGAAATTAATGAGGCCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACg                               >  2:2003063/1‑100 (MQ=255)                                                                    cGTTACTATATCGGCTGAAATTAATGAGGCCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAg                            <  1:454338/100‑1 (MQ=255)                                                                    cGTTACTATATCGGCTGAAATTAATGAGGCCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAg                            <  2:454338/100‑1 (MQ=255)                                                                     gTTACTAGATCGGCTGAAATTAATGAGGCCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGa                           <  2:1110793/100‑1 (MQ=255)                                                                        aCTATATCGGCTGAAATTAATGAGGCCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGAttt                        >  2:1748756/1‑100 (MQ=255)                                                                           atatCGGCTGAAATTAATGAGGCCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTcn                     <  2:2370122/100‑2 (MQ=255)                                                                           atatCGGCTGAAATTAATGAGGCCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTcc                     <  2:1845035/100‑1 (MQ=255)                                                                            tatCGGCTGAAATTAATGAGGCCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCt                    <  2:1010927/100‑1 (MQ=255)                                                                             atCGGCTGAAATTAATGAGGCCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCtt                   >  2:2481296/1‑100 (MQ=255)                                                                               cGGCTGAAATTAATGAGGCCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTAt                 <  2:1241031/100‑1 (MQ=255)                                                                                ggCTGAAATTAATGAGGCCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATc                >  2:651963/1‑100 (MQ=255)                                                                                ggCTGAAATTAATGAGGCCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATc                >  1:651963/1‑100 (MQ=255)                                                                                ggCTGAAATTAATGAGGCCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATc                >  2:2121226/1‑100 (MQ=255)                                                                                  cTGAAATTAATGAGGCCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAg              <  2:1051126/100‑1 (MQ=255)                                                                                  cTGAAATTAATGAGGCCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAg              >  2:358368/1‑100 (MQ=255)                                                                                  cTGAAATTAATGAGGCCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAg              >  1:358368/1‑100 (MQ=255)                                                                                   tGAAATTAATGAGGCCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCa               >  2:2270665/1‑98 (MQ=255)                                                                                   tGAAATTAATGAGGCCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAgg             >  2:2536794/1‑100 (MQ=255)                                                                                   tGAAATTAATGAGGCCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAgg             >  2:1101208/1‑100 (MQ=255)                                                                                    gAAATTAATGAGGCCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGga            >  2:1260451/1‑100 (MQ=255)                                                                                       aTTAATGAGGCCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGAt         >  2:2048632/1‑100 (MQ=255)                                                                                       aTTAATGAGGCCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGAt         >  2:1213386/1‑100 (MQ=255)                                                                                            tGAGGCCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCTTc    >  2:1357303/1‑100 (MQ=255)                                                                                             gAGGCCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCTTCt   >  1:544766/1‑100 (MQ=255)                                                                                             gAGGCCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCTTCt   >  2:544766/1‑100 (MQ=255)                                                                                             gAGGCCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCTTCt   <  2:1515776/100‑1 (MQ=255)                                                                                              aGGCCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCTTCTg  <  2:2410549/100‑1 (MQ=255)                                                                                                 |                                                                                                 GATGGCGATTATATAACCCACAAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCTTCTG  >  NC_000913/2810664‑2810856

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑