Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,338,928 C→A 100% L107F (TTG→TTT)  mlaE ← ABC transporter maintaining OM lipid asymmetry, inner membrane permease protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,338,9280CA100.0% 577.3 / NA 166L107F (TTG→TTT) mlaEABC transporter maintaining OM lipid asymmetry, inner membrane permease protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (74/92);  total (74/92)

Only 100 of 177 total aligned reads displayed.

CAGCGGATCCACCGCCATCATCTCCATACTGGAGAGTTGCTCTGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAACAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATACCCAGACTGGTTTCCGCACTATAAGTGGTCAGAACCAGAT  >  NC_000913/3338829‑3339025
                                                                                                   |                                                                                                 
cAGCGGATCCACCGCCATCATCTCCATACTGGAGAGTTGCTCTGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAaac                                                                                                   >  1:2329504/1‑100 (MQ=255)
  gCGGATCCACCGCCATCATCTCCATACTGGAGAGTTGCTCTGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCaaaaaa                                                                                                 <  1:685098/100‑1 (MQ=255)
   cGGATCCACCGCCATCATCTCCATACTGGAGAGTTGCTCTGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGTGCCGAACCAGCACGCCCGGCAaaaaac                                                                                                <  2:3167246/100‑1 (MQ=255)
      aTCCACCGCCATCATCTCCATACTGGAGAGTTGCTCTGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAaaaaacaac                                                                                             <  1:3100205/100‑1 (MQ=255)
       tCCACCGCCATCATCTCCATACTGGAGAGTTGCTCTGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAAcg                                                                                            <  2:1276847/100‑1 (MQ=255)
        ccACCGCCATCATCTCCATACTGGAGAGTTGCTCTGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAAcgc                                                                                           <  2:790039/100‑1 (MQ=255)
        ccACCGCCATCATCTCCATACTGGAGAGTTGCTCTGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAAcgc                                                                                           <  2:3337343/100‑1 (MQ=255)
           ccGCCATCATCTCCATACTGGAGAGTTGCTCTGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGc                                                                                        <  1:2493613/100‑1 (MQ=255)
             gCCATCATCTCCATACTGGAGAGTTGCTCTGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCaa                                                                                      >  1:1263973/1‑100 (MQ=255)
                  catcTCCATACTGGAGAGTTGCTCTGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAaccacc                                                                                 >  2:1342776/1‑100 (MQ=255)
                  catcTCCATACTGGAGAGTTGCTCTGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAaccacc                                                                                 <  2:1250204/100‑1 (MQ=255)
                    tctcCATACTGGAGAGTTGCTCTGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCgg                                                                               <  2:1005658/100‑1 (MQ=255)
                    tctcCATACTGGAGAGTTGCTCTGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCgg                                                                               >  1:1858804/1‑100 (MQ=255)
                     ctcCATACTGGAGAGTTGCTCTGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGc                                                                              <  2:444538/100‑1 (MQ=255)
                      tcCATACTGGAGAGTTGCTCTGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAACCAACGCGGCAACCACCGGcc                                                                             >  2:3233736/1‑100 (MQ=255)
                       ccATACTGGAGAGTTGCTCTATAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGccc                                                                            >  1:2283318/1‑100 (MQ=255)
                          tACTGGAGAGTTGCTCTGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAg                                                                         <  1:3442698/100‑1 (MQ=255)
                           aCTGGAGAGTTGCTCTGTAGCGCGCATCACGCCGAGTTCCGCGGTTAGCTCCTCACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCTCCAGt                                                                        <  2:2611597/100‑1 (MQ=255)
                            cTGGAGAGTTGCTCTGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGtt                                                                       >  1:3338240/1‑100 (MQ=255)
                             tGGAGGGTTGGTCTGTAGCGAGCATCAGGCCGATGTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGcccc                                                                           <  1:1160694/95‑1 (MQ=255)
                              ggAGAGTTGCTCTGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCa                                                                     >  1:802652/1‑100 (MQ=255)
                               gagaGTTGCTCTGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCAc                                                                    <  1:3316836/100‑1 (MQ=255)
                                agagTTGCGCTGTCGCCCCCATCAGCCCGATTTCTGCGGTTAGCGCCGAACCAGAACGCCCGGCAAAAAACAACGCGGCAACCAACGGACCCAGTTCa                                                                     >  1:2940434/1‑98 (MQ=255)
                                 gagTTGCTCTGTAGCGCGCCTCAGGCCGATGTCTGCCGTTCGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGc                                                                  <  1:297597/100‑1 (MQ=255)
                                 gagTTGCTCTGTAGCGCGCATCAGGCCGATTTCTGCGGTTATCGCCGAATCAGAACGACCGGCAAAACACAACGCGGCAACCACCCGCCCCAGTTGACGc                                                                  >  1:1776655/1‑100 (MQ=255)
                                 gagTTGCTCTGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGc                                                                  <  1:1839015/100‑1 (MQ=255)
                                 cgatctctctGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGc                                                                  <  2:1745226/94‑1 (MQ=255)
                                   gTTGCTCTGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAg                                                                >  2:1153675/1‑100 (MQ=255)
                                   gTTGCTCTGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAg                                                                >  2:2384800/1‑100 (MQ=255)
                                   gTTGCTCTGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAg                                                                >  2:612284/1‑100 (MQ=255)
                                    ttGCTCTGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGt                                                               <  2:2749862/100‑1 (MQ=255)
                                      gCTCTGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAg                                                             >  1:2742530/1‑100 (MQ=255)
                                       ctctGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAgatcg                                                            >  1:1745219/1‑96 (MQ=255)
                                       ctctGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAg                                                             >  1:3669257/1‑99 (MQ=255)
                                       ctctGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGc                                                            >  2:2392665/1‑100 (MQ=255)
                                       ctctGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGc                                                            >  2:1509801/1‑100 (MQ=255)
                                         ctGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGa                                                          <  1:587408/100‑1 (MQ=255)
                                          tGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGAt                                                         <  2:58380/100‑1 (MQ=255)
                                           gTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATa                                                        >  1:1698054/1‑100 (MQ=255)
                                           gTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATa                                                        <  1:40690/100‑1 (MQ=255)
                                              gcgcgcATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACg                                                     <  1:595309/100‑1 (MQ=255)
                                               cgcgcATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGc                                                    >  2:2810568/1‑100 (MQ=255)
                                                gcgcATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGcc                                                   <  1:1454972/100‑1 (MQ=255)
                                                   cATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAGCCACCGGCCCCAGTTCACGCAGTAGCGATAACGccacc                                                >  1:2699085/1‑100 (MQ=255)
                                                   cATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGccacc                                                >  1:3238623/1‑100 (MQ=255)
                                                    aTCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGccacca                                               <  1:1640418/100‑1 (MQ=255)
                                                     tCATGCCGATTTCTGCGGTTAGCGCCTAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAg                                              <  2:499285/100‑1 (MQ=255)
                                                     tCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAg                                              <  2:82287/100‑1 (MQ=255)
                                                     tCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACACCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAg                                              <  2:3554607/100‑1 (MQ=255)
                                                     tCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGAACAACAAAAAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAg                                              <  1:2432812/100‑1 (MQ=255)
                                                     tCAGGCCGATTTCTGCGGTTAGCGCCCAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAg                                              <  1:2189539/100‑1 (MQ=255)
                                                      cAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGc                                             >  1:1964213/1‑100 (MQ=255)
                                                      cAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGc                                             >  2:1228246/1‑100 (MQ=255)
                                                       aGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCa                                            <  1:1436986/100‑1 (MQ=255)
                                                        ggCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAACACACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCAt                                           >  1:3717561/1‑100 (MQ=255)
                                                        ggCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCAt                                           >  2:1209172/1‑100 (MQ=255)
                                                        ggCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCAt                                           >  1:3156284/1‑100 (MQ=255)
                                                        ggCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCAt                                           <  1:1000044/100‑1 (MQ=255)
                                                            gATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATAccc                                       <  2:127791/100‑1 (MQ=255)
                                                             aTTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATACCCa                                      >  1:790675/1‑100 (MQ=255)
                                                             aTTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATACCCa                                      >  2:851923/1‑100 (MQ=255)
                                                               ttCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATACCCAGa                                    >  2:988366/1‑100 (MQ=255)
                                                               ttCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGCCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATACCCAGa                                    >  1:2383565/1‑100 (MQ=255)
                                                                 cggCGGTTAGCGCCGANCCANCACGCCCGGCAAAAAACAACGCGGNAACNACCGGCCCCAGTTNACGCAGTNGCGATANCGCCNCCAGCATACCCAGAc                                   <  1:2278769/97‑1 (MQ=255)
                                                                  tGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATACCCAGACTg                                 <  2:1964223/100‑1 (MQ=255)
                                                                    cGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATACCCAGACTGGt                               >  1:84579/1‑100 (MQ=255)
                                                                     ggTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATACCCAGACTGGtt                              <  2:2042249/100‑1 (MQ=255)
                                                                     ggTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATACCCAGACTGGtt                              <  1:2529793/100‑1 (MQ=255)
                                                                        tAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATACCCAGACTGGTTTcc                           >  1:3289029/1‑100 (MQ=255)
                                                                        tAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATACCCAGACTGGTTTcc                           <  2:1117130/100‑1 (MQ=255)
                                                                         aGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATACCCAGACTGGTTTCCg                          >  2:1732175/1‑100 (MQ=255)
                                                                          gcgcCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATACCCAGACTGGTTTCCGc                         <  1:2392651/100‑1 (MQ=255)
                                                                          gcgcCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCACCATACCCAGACTGGTTTCCGc                         >  1:2891212/1‑100 (MQ=255)
                                                                           cgcCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATACCCAGACTGGTTTCCGc                         >  1:565350/1‑99 (MQ=255)
                                                                           cgcCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATACCCAGACTGGTTTCCGCa                        <  2:654834/100‑1 (MQ=255)
                                                                            nccGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATACCCAGACTGGTTTCCGCAc                       >  2:1512179/2‑100 (MQ=255)
                                                                            gcCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATACCCAGACTGGTTTCCGCAc                       >  1:2804188/1‑100 (MQ=255)
                                                                              cGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATACCCAGACTGGTTTCCGCACta                     >  2:3349085/1‑100 (MQ=255)
                                                                               gAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATACCCAGACTGGTTTCCGCACtat                    <  1:164546/100‑1 (MQ=255)
                                                                               gAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATACCCAGACTGGTTTCCGCACt                      <  1:2267316/98‑1 (MQ=255)
                                                                                aaCCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATACCCAGACTGGTTTCCGCACtata                   <  1:70213/100‑1 (MQ=255)
                                                                                aaCCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGACCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATACCCAGACTGGTTTccagatcgga                   >  2:3160117/1‑92 (MQ=255)
                                                                                 aCCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATACCCAGACTGGTTTCCGCACTATaa                  <  1:1342771/100‑1 (MQ=255)
                                                                                  ccAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATACCCAGACTGGTTTCCGCACTATAAg                 <  2:2674192/100‑1 (MQ=255)
                                                                                  ccAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCAAGTTCACGCAATAGCGATAACGCCACCAGCATACCCAGACTGGTTTCCGCACTATAAg                 >  1:767132/1‑100 (MQ=255)
                                                                                    aGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAATGCCACCAGCATACCCAGACTGGTTTCCGCACTATAAGTg               >  1:129530/1‑100 (MQ=255)
                                                                                     gCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATACCCAGACTGGTTTCCGCACTATAAGTgg              >  2:3697619/1‑100 (MQ=255)
                                                                                     gCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATACCCAGACTGGTTTCCGCACTATAAGTgg              >  1:1103771/1‑100 (MQ=255)
                                                                                      cACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATACCGCCACCAGCATACCCAGACTGGTTTCCCCACTATAAGTgct             <  2:2529095/100‑3 (MQ=255)
                                                                                       aCGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATACCCAGACTGGTTTCCGCACTATAAGTGGTc            <  2:790679/100‑1 (MQ=255)
                                                                                       aCGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATACCCAGACTGGTTTCCGCACTATAAGTGGTc            <  2:2026481/100‑1 (MQ=255)
                                                                                        cGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATACCCAGACTGGTTTCCGCACTATAAGTGGTc            <  1:880842/99‑1 (MQ=255)
                                                                                        cGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATACCCAGACTGGTTTCCGCACTATAAGTGGTCa           >  2:2853822/1‑100 (MQ=255)
                                                                                         gCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATACCCAGACTGGTTTCCGCACTATAAGTGGTCAg          >  2:1006783/1‑100 (MQ=255)
                                                                                          cccGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATACCCAGACTGGTTTCCGCACTATAAGTGGTCAGa         >  2:303420/1‑100 (MQ=255)
                                                                                            cGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATACCCAGACTGGTTTCCGCACTATAAGTGGTCAGAAc       <  2:3010779/100‑1 (MQ=255)
                                                                                            cGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATACCCAGACTGGTTTCCGCACTATAAGTGGTCAGAAc       <  2:1858814/100‑1 (MQ=255)
                                                                                             ggCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATACCCAGACTGGTTTCCGCACTATAAGTGGTCAGAAcc      <  1:988361/100‑1 (MQ=255)
                                                                                               cAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATACCCAGACTGGTTTCCGCACTATAAGTGGTCAGAACCAg    <  2:1700040/100‑1 (MQ=255)
                                                                                                 aacaacaacGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATACCCAGACTGGTTTCCGCACTATAAGTGGTCAGAACCAGAt  <  2:2329517/100‑1 (MQ=255)
                                                                                                   |                                                                                                 
CAGCGGATCCACCGCCATCATCTCCATACTGGAGAGTTGCTCTGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAACAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATACCCAGACTGGTTTCCGCACTATAAGTGGTCAGAACCAGAT  >  NC_000913/3338829‑3339025

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑