Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,705,971 C→A 25.8% intergenic (‑181/+127) dppB ← / ← dppA dipeptide/heme ABC transporter permease/dipeptide/heme ABC transporter periplasmic binding protein; dipeptide chemotaxis receptor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,705,9710CA25.8% 483.0 / 160.5 207intergenic (‑181/+127)dppB/dppAdipeptide/heme ABC transporter permease/dipeptide/heme ABC transporter periplasmic binding protein; dipeptide chemotaxis receptor
Reads supporting (aligned to +/- strand):  ref base C (65/87);  new base A (48/5);  total (113/94)
Fisher's exact test for biased strand distribution p-value = 2.90e-10
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.34e-01

Only 100 of 219 total aligned reads displayed.

GGGAATGAAGTATTGCTGCCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATT‑‑‑TTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCT  >  NC_000913/3705873‑3706070
                                                                                                  |                                                                                                           
gggAATGAAGTATTGCTGCCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATgcg                                                                                                            >  2:2951933/1‑100 (MQ=255)
gggAATGAAGTATTGCTGCCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATgcg                                                                                                            >  2:2676500/1‑100 (MQ=255)
gggAATGAAGTATTGCTGCCGGATGGCGCTGCACGCAGAGGCAAAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATgcg                                                                                                            >  2:3563220/1‑100 (MQ=255)
 ggAATGAAGTATTGCTGCCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATgcgg                                                                                                           >  2:655458/1‑100 (MQ=255)
 ggAATGAAGTATAGCTGCCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATgcgg                                                                                                           >  2:2461609/1‑100 (MQ=255)
  gAATGTAGTATTGCTGCCGGATGGCGCTGCACGCAGAGGCACTGCGCCGCCGGGTTTACAGATTGTTGACAAATTGCGCTTTGTTGATTCCGGATgcg                                                                                                            >  1:2226813/1‑98 (MQ=38)
  gAATGAAGTATTGCTGCCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATgcggc                                                                                                          >  2:2154484/1‑100 (MQ=255)
   aaTGAAGTATTGCTGCCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATgcggcg                                                                                                         <  2:3091417/100‑1 (MQ=255)
     tGAAGTATTGCTGCCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGtt                                                                                                       >  2:427861/1‑100 (MQ=255)
     tGAAGTATTGCTGCCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGtt                                                                                                       <  2:2383351/100‑1 (MQ=255)
        aGTATTGCTGCCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATgcggcataaac                                                                                                    <  2:3417892/100‑7 (MQ=255)
         gTATTGCTGCCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACg                                                                                                   <  1:280471/100‑1 (MQ=255)
         gTATTGCTGCCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACg                                                                                                   <  1:1562972/100‑1 (MQ=255)
          tATTGCTGCCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTAACAAAGTGCGATTTGTTTATGCCGGATGCGGCGTTAACGc                                                                                                  >  1:836643/1‑100 (MQ=255)
           aTTGCTGCCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGcc                                                                                                 <  1:3643778/100‑1 (MQ=255)
           aTTGCTGCCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGcc                                                                                                 <  1:2523573/100‑1 (MQ=255)
            ttGCTGCCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCt                                                                                                <  2:2869453/100‑1 (MQ=255)
            ttGCTGCCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCt                                                                                                <  2:3154222/100‑1 (MQ=255)
            ttGCTGCCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCt                                                                                                <  1:982211/100‑1 (MQ=255)
             tgctgcCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCtt                                                                                               >  1:1022670/1‑100 (MQ=255)
              gctgcCGGATGGCGCTGCACGCAGAGGCACAGCGCCGCCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTa                                                                                              <  1:1093105/100‑1 (MQ=255)
                tgcCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATc                                                                                            >  1:3016339/1‑100 (MQ=255)
                 gcCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATcc                                                                                           >  2:2066277/1‑100 (MQ=255)
                    ggATGGCGCTACACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTCTACGCCGGATGCGGCGTTAACGCCTTATCCGGc                                                                                        <  1:2461596/100‑1 (MQ=255)
                     gATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATAAGGCGTTAACGCCGCATCCGGca                                                                                       <  1:727678/100‑2 (MQ=37)
                      aTGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCt                                                                                      <  2:382467/100‑1 (MQ=255)
                      aTGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCt                                                                                      <  1:775701/100‑1 (MQ=255)
                      aTGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATAAGGCGTTAACGCCGCATCCGGcat                                                                                      <  1:3000303/100‑3 (MQ=25)
                         gcgcTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATAAGGCGTTAACGCCGCATCCGGcat                                                                                      <  2:1302352/97‑3 (MQ=25)
                          cgcTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGGCAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACaa                                                                                <  2:1804473/100‑1 (MQ=255)
                              gCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGTGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATc                                                                            >  2:1201301/1‑100 (MQ=255)
                               cACGCAGAGGCACAGCGCCACCGGGTATACATATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCg                                                                           <  2:505950/100‑1 (MQ=255)
                               cACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGACCGCTGCGACTTTAACGCCTTCTCCGCCATACAAAAACg                                                                           >  1:121753/1‑100 (MQ=21)
                                 cGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGt                                                                          <  1:2344518/99‑1 (MQ=255)
                                 cGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTg                                                                         <  2:1893263/100‑1 (MQ=255)
                                 cGCAGAGGCACAGCACCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATACCGGATGCGGCGTTAACGCCTTATCCGACCTACAAAATCGTg                                                                         >  1:1348269/1‑100 (MQ=255)
                                  gCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGc                                                                        <  1:2943993/100‑1 (MQ=255)
                                   cAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGTCCTACAAAATCGTGCa                                                                       >  1:3459258/1‑100 (MQ=255)
                                   cAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCa                                                                       <  2:2011895/100‑1 (MQ=255)
                                          acaGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCaa                                                                >  2:3575216/1‑100 (MQ=255)
                                           caGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAAt                                                               <  1:2910280/100‑1 (MQ=255)
                                             gcgcCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATAc                                                             <  1:1746054/100‑1 (MQ=255)
                                               gcCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACAt                                                           <  2:2264695/100‑1 (MQ=255)
                                               gcCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACAt                                                           >  1:1187350/1‑100 (MQ=255)
                                                ccACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACAtt                                                          >  1:112978/1‑100 (MQ=255)
                                                 cACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCAGGATGTGGCGTTAACTCTTTATCCGGCCTACAAAATCGTGCAAATTCAATACAtt                                                          <  1:883397/99‑1 (MQ=18)
                                                  cgatcttataCAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGc                                                        <  2:1431508/94‑1 (MQ=255)
                                                     gggTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGa                                                     >  1:3203892/1‑100 (MQ=255)
                                                        tataCAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAAtt                                                 >  1:1888014/1‑100 (MQ=255)
                                                         ataCAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAAtt‑‑‑t                                             <  1:3570604/100‑1 (MQ=255)
                                                            cAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATT‑‑‑TTC‑‑g                                        <  1:2832051/100‑1 (MQ=255)
                                                            cAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATT‑‑‑TTC‑‑g                                        <  2:1187356/100‑1 (MQ=255)
                                                             aGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATT‑‑‑TTC‑‑Gt                                       >  2:3621596/1‑100 (MQ=255)
                                                              gATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATT‑‑‑TTC‑‑GTa                                      >  2:414958/1‑100 (MQ=255)
                                                              gATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATT‑‑‑TTC‑‑GTa                                      <  1:3621568/100‑1 (MQ=255)
                                                                gcataaaCAAAGCGCACTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATT‑‑‑TTC‑‑GTAgg                                    >  1:2204182/7‑100 (MQ=18)
                                                                    tgACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATT‑‑‑TTC‑‑GTAGGCCTg                                >  2:282792/1‑100 (MQ=255)
                                                                     gACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATT‑‑‑TTC‑‑GTAGGCCTGa                               >  1:2021679/1‑100 (MQ=255)
                                                                      aCAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATT‑‑‑TTC‑‑GTAGGCCTGAt                              <  1:2096882/100‑1 (MQ=255)
                                                                      aCAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATT‑‑‑TTC‑‑GTAGGCCTGAt                              <  1:272825/100‑1 (MQ=255)
                                                                         aaGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATT‑‑‑TTC‑‑GTAGGCCTGATAAg                           >  2:3732283/1‑100 (MQ=255)
                                                                          aGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATT‑‑‑TTC‑‑GTAGGCCTGATAAGc                          >  2:2984976/1‑100 (MQ=255)
                                                                          aGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATT‑‑‑TTC‑‑GTAGGCCTGATAAGc                          <  1:1249325/100‑1 (MQ=255)
                                                                          aGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATT‑‑‑TTC‑‑GTAGGCCTGATAAGc                          <  1:1661067/100‑1 (MQ=255)
                                                                            tGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATT‑‑‑TTC‑‑GTAGGCCTGATAAGCgg                        >  2:2369793/1‑100 (MQ=255)
                                                                             gcgcTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATT‑‑‑TTC‑‑GTAGCCCTGATAAGCGGa                       <  2:2538387/100‑1 (MQ=255)
                                                                              cgcTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTCCACAATCGTGCAAATTCAATACATTGCAGAATT‑‑‑TTC‑‑GTAGGCCTGATAAGCGGAg                      <  1:308987/100‑1 (MQ=255)
                                                                              cgcTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATT‑‑‑TTC‑‑GTAGGCCTGATAAGCGGAg                      >  2:3616599/1‑100 (MQ=255)
                                                                              cgcTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATT‑‑‑TTC‑‑GTAGGCCTGATAAGCGGAg                      <  2:2601910/100‑1 (MQ=255)
                                                                               gcTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATT‑‑‑TTC‑‑GTAGGCCTGATAAGCGGAgc                     <  1:1773930/100‑1 (MQ=255)
                                                                                ttccgatctggCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAGATCGTGCAAATTCAATATATTGCATGAGC‑‑‑CAC‑‑GTAGGCCTGATAAGCGTAgcg                    <  1:2576287/90‑1 (MQ=17)
                                                                                cTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATT‑‑‑TTC‑‑GTAGGCCTGATAAGCGGAgcg                    >  1:372536/1‑100 (MQ=255)
                                                                                cTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATT‑‑‑TTC‑‑GTAGGCCTGATAAGCGGAgcg                    >  1:1975033/1‑100 (MQ=255)
                                                                                 tttGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATT‑‑‑TTC‑‑GTAGGCCTGATAAGCGGAgcgc                   >  1:2856861/1‑100 (MQ=255)
                                                                                  ttGTGTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAAGAGTGCTAATTAAATACATTGCTGTATT‑‑‑TTC‑‑GTAGGCCTGATAAGCgg                        <  1:440247/94‑1 (MQ=17)
                                                                                    ttgtaggCCGGATAAGGCGTTCACGCCGCATCCGGCATACAAAAATCTTGCCAATTCAATATATTGCAGGACC‑‑‑AT‑‑GTAGGCCTGATAAGCGTAGCGCATc                >  2:251765/7‑100 (MQ=18)
                                                                                    gTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATT‑‑‑TTC‑‑GTAGGCCTGATAAGCGGAGCGCATc                >  2:1916605/1‑100 (MQ=255)
                                                                                     tttATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATT‑‑‑TTC‑‑GTAGGCCTGATAAGCGGAGCGCATCa               <  1:1201295/100‑1 (MQ=255)
                                                                                     tttATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATT‑‑‑TTC‑‑GTAGGCCTGATAAGCGGAGCGCATCa               <  2:3181627/100‑1 (MQ=255)
                                                                                      ttATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATT‑‑‑TTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAg              <  1:1171033/100‑1 (MQ=255)
                                                                                      gtaggCCGGATAAGGCGTTCACGCCGCATCCGGCATGACAAAAACCTGCAAATTCAATATATTGCAGGATA‑‑‑CGT‑‑GTAGGCCTGATAAGCGTAGCGCATCa               >  2:1528971/5‑100 (MQ=17)
                                                                                      gtaggCCGGATAAGGCGTTCACGCCGCATCCGGCATACAAAAACCTGCAAATTCAATATATTGCAGGATA‑‑‑CGT‑‑GTAGGCCTGATAAGCGTAGCGCATCAg              >  1:494504/5‑100 (MQ=18)
                                                                                       taggCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAAAACCTGCAAATTCAATATATTGCAGGATA‑‑‑CGT‑‑GTAGGCCTGATAAGCGTAGCGCATCAgg             >  2:1036689/4‑100 (MQ=18)
                                                                                        aggCCGGATAAGGCGTTTACGCCGCATCCGGCCTACAAAATCGTGCTAATTCAATATATTGCATAAAC‑‑‑CTT‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGc            >  2:3550985/3‑100 (MQ=17)
                                                                                        aggCCGGATAAGGCGTTTACGCCGCATCCGGCCTACAAAAGCGTGCAAATTCAATACATTGCATGGGC‑‑‑CAT‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGc            >  1:2568609/3‑100 (MQ=17)
                                                                                        aggCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAAGTCGTATAAATTCAATGTATTGCAGTTTA‑‑‑TTG‑‑GTAGGCCTGATAAGCGCAGCGCATCAGGc            >  2:761071/3‑100 (MQ=21)
                                                                                        aggCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAAACTTTGCAAATTCAATATATTGC‑‑AATC‑‑‑TCCGTGTAGGCCTGATAAGCGTAGCGCATCAGGc            >  2:3500583/3‑100 (MQ=18)
                                                                                         ggCCGGATAAGGCGTTTACGCCGCATCCGGCCTACAAAAGCATGCAAATTCAATATATTGCAGAGTT‑‑‑CAC‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCa           >  1:3335966/2‑100 (MQ=17)
                                                                                         ggCCGGATAAGGCGTTTACGCCGCATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGC‑‑AATTCAACTT‑‑GTAGGCCTGATAAGCGCAGCGCATCAGGc            >  2:1584065/2‑100 (MQ=18)
                                                                                         ggCCGGATAAGGCGTTCACTCCGCATCCGGCCTACAAAAGCACGCAAATTCAATATATTGCAGAGAT‑‑‑TGC‑‑GTAGGCCTGATAAGCGTAGCGCATCAgg             >  2:2966949/2‑98 (MQ=14)
                                                                                         ggCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAAAACATGCAAATTCAATAGATTGCAGAGATT‑‑‑AT‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCa           >  2:1510802/2‑100 (MQ=17)
                                                                                         ggCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAAAACATGCAAATTCAATAGATTGCAGAGAT‑‑‑TAT‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCa           >  2:2698/2‑100 (MQ=17)
                                                                                         ggCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAATCATGCAAATCCAAAATATTGCAGGAGT‑‑‑TAT‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCAt          >  2:1687542/2‑100 (MQ=21)
                                                                                          gCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATT‑‑‑TTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCAt          <  2:3267846/100‑1 (MQ=255)
                                                                                          gCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATT‑‑‑TTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCAt          >  2:17402/1‑100 (MQ=255)
                                                                                          gCCGGATAAGGCGTTCACTCCGCATCCGGCCTACAAAAGCACGCAAATTCAATATATTGCAGAGAT‑‑‑TGC‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCaa          >  2:2427896/1‑99 (MQ=14)
                                                                                          gCCGGATAAGGCGTTCACTCCGCATCCGGCCTACAAAAGCACGCAAATTCAATATATTGCAGAAAT‑‑‑TGC‑‑GTAGGCCTGATAAGCGTAGCGCATCAGACaa          >  2:177405/1‑99 (MQ=11)
                                                                                          gCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAAATCGCTTAAATTCAATATATTGCACCATC‑‑‑CTC‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCaa          >  2:1096657/1‑99 (MQ=21)
                                                                                           ccGGATAAGGCGTTTACGCCGCATCTGGCCTACAAATTCTTCCAAATTCAATATATTGCAGAAAT‑‑‑CAT‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCaat         >  1:1059579/1‑98 (MQ=17)
                                                                                             ggATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATT‑‑‑TTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCAtttt       <  1:1389415/100‑1 (MQ=255)
                                                                                                  |                                                                                                           
GGGAATGAAGTATTGCTGCCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATT‑‑‑TTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCT  >  NC_000913/3705873‑3706070

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: