BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,966,951	A→G	V495A (GTC→GCC)	ptsP ←	PEP‑protein phosphotransferase enzyme I; GAF domain containing protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,966,951	0	A	G	100.0%	401.6 / NA	107	V495A (GTC→GCC)	ptsP	PEP‑protein phosphotransferase enzyme I; GAF domain containing protein
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (61/46); total (61/46)

Only 100 of 107 total aligned reads displayed.

ATGCGAATCCCACGCCAACCCAGGCATGGATTCTCTTCGCTGATCGGCATGTAAGGCAGCTGCTTATCTGCTCCGACATCCAGCGTACGCAAGGTGACGGGTTTATCATTGAACATTTGCAGCATCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGGAAACCACTTTGCAGCATGAATGGGAT  >  NC_000913/2966855‑2967042

aTGCGAATCCCACGCCAACCCAGGCATGGATTCTCTCCGCTGATCGGCATGTAAGGCAGCTGCTTATCTGCTCCGACATCCAGCGTACGCAAGGTGGCgg                                                                                          <  2:2474522/100‑1 (MQ=255)

 tGCGAATCCCACGCCAACCCAGGCATGGATTCTCTTCGCTGATCGGCATGTAAGGCAGCTGCTTATCTGCTCCGACATCCAGCGTACGCAAGGTGGCggg                                                                                         >  1:2689326/1‑100 (MQ=255)

 tGCGAATCCCACGCCAACCCAGGCATGGATTCTCTTCGCTGATCGGCATGTAAGGCAGCTGCTTATCTGCTCCGACATCCAGCGTACGCAAGGTGGCgg                                                                                          >  1:2728155/1‑99 (MQ=255)

   ttccgatctcGCCAACCCAGGCATGGATTCTCTTCGCTGATCGGCATGTAAGGCAGCTGCTTATCTGCTCCGACATCCAGCGTACGCAAGGTGGCGGGtn                                                                                       <  2:1106843/91‑2 (MQ=255)

   cGAATCCCACGCCAACCCAGGCATGGATTCTCTTCGCTGATCGGCATGTAAGGCAGCTGCTTATCTGCTCCGACATCCAGCGTACGCAAGGTGGCGGGtt                                                                                       <  2:1261380/100‑1 (MQ=255)

   cGAATCCCACGCCAACCCAGGCATGGATTCTCTTCGCTGATCGGCATGTAAGGCAGCTGCTTATCTGCTCCGACATCCAGCGTACGCAAGGTGGCGGGtt                                                                                       <  1:1675760/100‑1 (MQ=255)

    gAATCCCACGCCAACCCAGGCATGGATTCTCTTCGCTGATCGGCATGTAAGGCAGCTGCTTATCTGCTCCGACATCCAGCGTACGCAAGGTGGCGGGttt                                                                                      >  1:2324635/1‑100 (MQ=255)

    gAATCCCACGCCAACCCAGGCATGGATTCTCTTCGCTGATCGGCATGTAAGGCAGCTGCTTATCTGCTCCGACATCCAGCGTACGCAAGGTGGCGGGtt                                                                                       <  1:1675555/99‑1 (MQ=255)

      aTCCCCCGCCAACCCAGGCATGGATTCTCTTCGCTGATCGGCATGTAAGGCAGCTGCTTATCTGATCCGACATCCAGCGTACGCAAGGTGGCGGGtt                                                                                       <  1:789898/97‑1 (MQ=255)

      aTCCCACGCCAACCCAGGCATGGATTCTCTTCGCTGATCGGCATGTAAGGCAGCTGCTTATCTGCTCCGACATCCAGCGTACGCAAGGTGGCGGGTTTAt                                                                                    >  1:922240/1‑100 (MQ=255)

      aTCCCACGCCAACCCAGGCATGGATTCTCTTCGCTGATCGGCATGTAAGGCAGCTGCTTATCTGCTCCGACATCCAGCGTACGCAAGGTGGCGGGTTTAt                                                                                    >  2:1531500/1‑100 (MQ=255)

       tCCCACGCCAACCCAGGCATGGATTCTCTTCGCTGATCGGCATGTAAGGCAGCTGCTTATCTGCTCCGACATCCAGCGTACGCAAGGTGGCGGGTTTATc                                                                                   <  2:1293165/100‑1 (MQ=255)

        cccACGCCAACCCAGGCATGGATTCTCTTCGCTGATCGGCATGTAAGGCAGCTGCTTATCTGCTCCGACATCCAGCGTACGCAAGGTGGCGGGTTTATCa                                                                                  >  2:292420/1‑100 (MQ=255)

         ccACGCCAACCCAGGCATGGATTCTCTTCGCTGATCGGCATGTAAGGCAGCTGCTTATCTGCTCCGACATCCAGCGTACGCAAGGTGGCGGGTTTATCAt                                                                                 >  1:1029212/1‑100 (MQ=255)

         ccACGCCAACCCAGGCATGGATTCTCTTCGCTGATCGGCATGTAAGGCAGCTGCTTATCTGCTCCGACATCCAGCGTACGCAAGGTGGCGGGTTTATCAt                                                                                 >  1:1539356/1‑100 (MQ=255)

          cACGCCAACCCAGGCATGGATTCTCTTCGCTGATCGGCATGTAAGGCAGCTGCTTATCTGCTCCGACATCCAGCGTACGCAAGGTGGCGGGTTTATCAtt                                                                                >  2:1375403/1‑100 (MQ=255)

          cACGCCAACCCAGGCATGGATTCTCTTCGCTGATCGGCATGTAAGGCAGCTGCTTATCTGCTCCGACATCCAGCGTACGCAAGGTGGCGGGTTTATCAtt                                                                                >  1:2091739/1‑100 (MQ=255)

            ngCCAACCCAGGCATGGATTCTCTTCGCTGATCGGCATGTAAGGCAGCTGCTTATCTGCTCCGACATCCAGCGTACGCAAGGTGGCGGGttagatcggaa                                                                              >  1:1106839/2‑91 (MQ=255)

              ccAACCCAGGCATGGATTCTCTTCGCTGATCGGCATGTAAGGCAGCTGCTTATCTGCTCCGACATCCAGCGTACGCAAGGTGGCGGGTTTATCATTGAAc                                                                            >  1:485482/1‑100 (MQ=255)

              ccAACCCAGGCATGGATTCTCTTCGCTGATCGGCATGTAAGGCAGCTGCTTATCTGCTCCGACATCCAGCGTACGCAAGGTGGCGGGTTTATCATTGAAc                                                                            <  1:8586/100‑1 (MQ=255)

                 aCCCAGGCATGGATTCTCTTCGCTGATCGGCATGTAAGGCAGCTGCTTATCTGCTCCGACATCCAGCGTACGCAAGGTGGCGGGTTTATCATTGAACAtt                                                                         <  1:771350/100‑1 (MQ=255)

                  cccAGGCATGGATTCTCTTCGCTGATCGGCATGTAAGGCAGCTGCTTATCTGCTCCGACATCCAGCGTACGCAAGGTGGCGGGTTTATCATTGAACAttt                                                                        >  2:462885/1‑100 (MQ=255)

                   ccAGGCATGGATTCTCTTCGCTGATCGGCATGTAAGGCAGCTGCTTATCTGCTCCGACATCCAGCGTACGCAAGGTGGCGGGTTTATCATTGAACATTTg                                                                       <  1:635186/100‑1 (MQ=255)

                    cAGGCATGGATTCTCTTCGCTGATCGGCATGTAAGGCAGCTGCTTATCTGCTCCGACATCCAGCGTACGCAAGGTGGCGGGTTTATCATTGAACATTTgc                                                                      >  1:2737472/1‑100 (MQ=255)

                    cAGGCATGGATTCTCTTCGCTGATCGGCATGTAAGGCAGCTGCTTATCTGCTCCGACATCCAGCGTACGCAAGGTGGCGGGTTTATCATTGAACATTTgc                                                                      >  1:761870/1‑100 (MQ=255)

                      ggCATGGATTCTCTTCGCTGATCGGCATGTAAGGCAGCTGCTTATCTGCTCCGACATCCAGCGTACGCAAGGTGGCGGGTTTATCATTGAACATTTgcag                                                                    <  2:1724117/100‑1 (MQ=255)

                      ggCATGGATTCTCTTCGCTGATCGGCATGTAAGGCAGCTGCTTATCTGCTCCGACATCCAGCGTACGCAAGGTGGCGGGTTTATCATTGAACATTTgcag                                                                    <  1:1138385/100‑1 (MQ=255)

                      ggCATGGATTCTCTTCGCTGATCGGCATGTAAGGCAGCTGCTTATCTGCTCCGACATCCAGCGTACGCAAGGTGGCGGGTTTATCATTGAACATTTgcag                                                                    <  2:1634805/100‑1 (MQ=255)

                      ggCATGGATTCTCTTCGCTGATCGGCATGTAAGGCAGCTGCTTATCTGCTCCGACATCCAGCGTACGCAAGGTGGCGGGTTTATCATTGAACATTTgcag                                                                    >  1:2902656/1‑100 (MQ=255)

                        cATGGATTCTCTTCGCTGATCGGCATGTAAGGCAGCTGCTTATCTGCTCCGACATCCAGCGTACGCAAGGTGGCGGGTTTATCATTGAACATTTgcagca                                                                  <  1:1363983/100‑1 (MQ=255)

                        cATGGATTCTCTTCGCTGATCGGCATGTAAGGCAGCTGCTTATCTGCTCCGACATCCAGCGTACGCAAGGTGGCGGGTTTATCATTGAACATTTgcagca                                                                  >  1:1504996/1‑100 (MQ=255)

                          tGGATTCTCTTCGCTGATCGGCATGTAAGGCAGCTGCTTATCTGCTCCGACATCCAGCGTACGCAAGGTGGCGGGTTTATCATTGAACATTTGCAGCATc                                                                <  1:2460678/100‑1 (MQ=255)

                          tGGATTCTCTTCGCTGATCGGCATGTAAGGCAGCTGCTTATCTGCTCCGACATCCAGCGTACGCAAGGTGGCGGGTTTATCATTGAACATTTGCAGCATc                                                                >  1:983520/1‑100 (MQ=255)

                           ggATTCTCTTCGCTGATCGGCATGTAAGGCAGCTGCTTATCTGCTCCGACATCCAGCGTACGCAAGGTGGCGGGTTTATCATTGAACATTTGCAGCATcc                                                               >  2:812336/1‑100 (MQ=255)

                           ggATTCTCTTCGCTGATCGGCATGTAAGGCAGCTGCTTATCTGCTCCGACATCCAGCGTACGCAAGGTGGCGGGTTTATCATTGAACATTTGCAGCATcc                                                               >  1:656357/1‑100 (MQ=255)

                           ggATTCTCTTCGCTGATCGGCATGTAAGGCAGCTGCTTATCTGCTCCGACATCCAGCGTACGCAAGGTGGCGGGTTTATCATTGAACATTTGCAGCATca                                                               >  2:2460694/1‑99 (MQ=255)

                            gATTCTCTTCGCTGATCGGCATGTAAGGCAGCTGCTTATCTGCTCCGACATCCAGCGTACGCAAGGTGGCGGGTTTATCATTGAACATTTGCAGCATccc                                                              >  1:1289463/1‑100 (MQ=255)

                             aTTCTCTTCGCTGATCGGCATGTAAGGCAGCTGCTTATCTGCTCCGACATCCAGCGTACGCAAGGTGGCGGGTTTATCATTGAACATTCGCAGAATcccc                                                             >  1:1034246/1‑100 (MQ=255)

                              ttCTCTTCGCTGATCGGCATGTAAGGCAGCTGCTTATCTGCTCCGACATCCAGCGTACGCAAGGTGGCGGGTTTATCATTGAACATTTGCAGCATCCCCt                                                            >  1:1263730/1‑100 (MQ=255)

                              ttCTCTTCGCTGATCGGCATGTAAGGCAGCTGCTTATCTGCTCCGACATCCAGCGTACGCAAGGTGGCGGGTTTATCATTGAACATTTGCAGCATCCCCt                                                            >  2:2639888/1‑100 (MQ=255)

                              ttCTCTTCGCTGATCGGCATGTAAGGCAGCTGCTTATCTGCTCCGACATCCAGCGTACGCAAGGTGGCGGGTTTATCATTGAACATTTGCAGCATCCCCt                                                            <  2:1013407/100‑1 (MQ=255)

                                 tctTCGCTGATCGGCATGTAAGGCAGCTGCTTATCTGCTCCGACATCCAGCGTACGCAAGGTGGCGGGTTTATCATTGAACATTTGCAGCATCCCCTGa                                                          <  1:1197137/99‑1 (MQ=255)

                                 tctTCGCTGATCGGCATGTAAGGCAGCTGCTTATCTGCTCCGACATCCAGCGTACGCAAGGTGGCGGGTTTATCATTGAACATTTGCAGCATCCCCTGAt                                                         <  2:1645911/100‑1 (MQ=255)

                                 tctTCGCTGATCGGCATGTAAGGCAGCTGCTTATCTGCTCCGACATCCAGCGTACGCAAGGTGGCGGGTTTATCATTGAACATTTGCAGCATCCCCTGAt                                                         <  2:2739782/100‑1 (MQ=255)

                                 tctTCGCCGATCGGCATGTAAGGCAGCTGCTTATCTGCTCCGACATCCAGCGTACGCAAGGTGGCGGGTTTATCATTGAACATTTGCAGCATCCCCTGAt                                                         >  1:254532/1‑100 (MQ=255)

                                  ctTCGCTGATCGGCATGTAAGGCAGCTGCTTATCTGCTCCGACATCCAGCGTACGCAAGGTGGCGGGTTTATCATTGAACATTTGCAGCATCCCCTGATa                                                        >  1:1754666/1‑100 (MQ=255)

                                  ctTCGCTGATCGGCATGTAAGGCAGCTGCTTATCTGCTCCGACATCCAGCGTACGCAAGGTGGCGGGTTTATCATTGAACATTTGCAGCATCCCCTGATa                                                        <  2:2607795/100‑1 (MQ=255)

                                    tCGCTGATCGGCATGTAAGGCAGCTGCTTATCTGCTCCGACATCCAGCGTACGCAAGGTGGCGGGTTTATCATTGAACATTTGCAGCATCCCCTGATACt                                                      <  2:2107194/100‑1 (MQ=255)

                                     cGCTGATCGGCATGTAAGGCAGCTGCTTATCTGCTCCGACATCCAGCGTACGCAAGGTGGCGGGTTTATCATTGAACATTTGCAGCATCCCCTGATACTg                                                     >  2:3052877/1‑100 (MQ=255)

                                       cTGATCGGCATGTAAGGCAGCTGCTTATCTGCTCCGACATCCAGCGTACGCAAGGTGGCGGGTTTATCATTGAACATTTGCAGCATCCCCTGATACTgcg                                                   >  2:2818534/1‑100 (MQ=255)

                                       cTGATCGGCATGTAAGGCAGCTGCTTATCTGCTCCGACATCCAGCGTACGCAAGGTGGCGGGTTTATCATTGAACATTTGCAGCATCCCCTGATACTgcg                                                   >  2:2260433/1‑100 (MQ=255)

                                       cTGATCGGCATGTAAGGCAGCTGCTTATCTGCTCCGACATCCAGCGTACGCAAGGTGGCGGGTTTATCATTGAACATTTGCAGCATCCCCTGATACTgcg                                                   >  2:2121145/1‑100 (MQ=255)

                                         gATCGGCATGTAAGGCAGCTGCTTATCTGCTCCGACATCCAGCGTACGCAAGGTGGCGGGTTTATCATTGAACATTTGCAGCATCCCCTGATACTTCGcc                                                 >  2:134604/1‑100 (MQ=255)

                                         gATCGGCATGTAAGGCAGCTGCTTATCTGCTCCGACATCCAGCGTACGCAAGGTGGCGGGTTTATCATTGAACATTTGCAGCATCCCCTGATACTGCGcc                                                 <  2:584677/100‑1 (MQ=255)

                                         gATCGGCATGTAAGGCAGCTGCTTATCTGCTCCGACATCCAGCGTACGCAAGGTGGCGGGTTTATCATTGAACATTTGCAGCATCCCCTGATACTGCGcc                                                 <  2:2346055/100‑1 (MQ=255)

                                         gATCGGCATGTAAGGCAGCTGCTTATCTGCTCCGACATCCAGCGTACGCAAGGTGGCGGGTTTATCATTGAACATTTGCAGCATCCCCTGATACTGCGcc                                                 <  2:2576734/100‑1 (MQ=255)

                                             ggCATGTAAGGCGGCTGCTTATCTGCTCCGACATCCAGCGTACGCAAGGTGGCGGGTTTATCATTGAACATTTGCAGCATCCCCTGATACTGCGCCACCt                                             >  2:2309957/1‑100 (MQ=255)

                                             ggCATGTAAGGCAGCTGCTTATCTGCTCCGACATCCAGCGTACGCAAGGTGGCGGGTTTATCATTGAACATTTGCAGCATCCCCTGATACTGCGCCACCt                                             >  2:349149/1‑100 (MQ=255)

                                               cATGTAAGGCAGCTGCTTATCTGCTCCGACATCCAGCGTACGCAAGGTGGCGGGTTTATCATTGAACATTTGCAGCATCCCCTGATACTGCGCCACCTGt                                           >  2:3233914/1‑100 (MQ=255)

                                               cATGTAAGGCAGCTGCTTATCTGCTCCGACATCCAGCGTACGCAAGGTGGCGGGTTTATCATTGAACATTTGCAGCATCCCCTGATACTGCGCCACCTGt                                           <  2:1289467/100‑1 (MQ=255)

                                                aTGTAAGGCAGCTGCTTATCTGCTCCGACATCCAGCGTACGCAAGGTGGCGGGTTTATCATTGAACATTTGCAGCATCCCCTGATACTGCGCCACCTGtt                                          >  1:1360019/1‑100 (MQ=255)

                                                 tGTAAGGCAGCTGCTTATCTGCTCCGACATCCAGCGTACGCAAGGTGGCGGGTTTATCATTGAACATTTGCAGCATCCCCTGATACTGCGCCACCTGttc                                         >  2:2716135/1‑100 (MQ=255)

                                                 tGTAAGGCAGCTGCTTATCTGCTCCGACATCCAGCGTACGCAAGGTGGCGGGTTTATCATTGAACATTTGCAGCATCCCCTGATACTGCGCCACCTGttc                                         >  1:2101442/1‑100 (MQ=255)

                                                  gTAAGGCAGCTGCTTATCTGCTCCGACATCCAGCGTACGCAAGGTGGCGGGTTTATCATTGAACATTTGCAGCATCCCCTGATACTGCGCCACCTGttct                                        <  2:3229300/100‑1 (MQ=255)

                                                     aGGCAGCTGCTTATCTGCTCCGACATCCAGCGTACGCAAGGTGGCGGGTTTATCATTGAACATTTGCAGCATCCCCTGATACTGCGCCACCTGttcttct                                     >  1:174971/1‑100 (MQ=255)

                                                     aGGCAGCTGCTTATCTGCTCCGACATCCAGCGTACGCAAGGTGGCGGGTTTATCATTGAACATTTGCAGCATCCCCTGATACTGCGCCACCTGttcttct                                     <  1:396611/100‑1 (MQ=255)

                                                     aGGCAGCTGCTTATCTGCTCCGACATCCAGCGTACGCAAGGTGGCGGGTTTATCATTGAACATTTGCAGCATCCCCTGATACTGCGCCACCTGttcttct                                     <  2:2221755/100‑1 (MQ=255)

                                                       gCAGCTGCTTATCTGCTCCGACATCCAGCGTACGCAAGGTGGCGGGTTTATCATTGAACATTTGCAGCATCCCCTGATACTGCGCCACCTGttcttcttc                                   <  1:2795040/100‑1 (MQ=255)

                                                        cAGCTGCTTATCTGCTCCGACATCCAGCGTACGCAAGGTGGCGGGTTTATCATTGAACATTTGCAGCATCCCCTGATACTGCGCCACCTGTTCTTCTTcc                                  <  2:276932/100‑1 (MQ=255)

                                                        cAGCTGCTTATCTGCTCCGACATCCAGCGTACGCAAGGTGGCGGGTTTATCATTGAACATTTGCAGCATCCCCTGATACTGCGCCACCTGTTCTTCTTcc                                  >  2:1182004/1‑100 (MQ=255)

                                                         aGCTGCTTATCTGCTCCGACATCCAGCGTACGCAAGGTGGCGGGTTTATCATTGAACATTTGCAGCATCCCCTGATACTGCGCCACCTGTTCTTCTTCCg                                 >  2:1141695/1‑100 (MQ=255)

                                                         aGCTGCTTATCTGCTCCGACATCCAGCGTACGCAAGGTGGCGGGTTTATCATTGAACATTTGCAGCATCCCCTGATACTGCGCCACCTGTTCTTCTTCCg                                 >  1:316830/1‑100 (MQ=255)

                                                          gctgctTATCTGCTCCGACATCCAGCGTACGCAAGGTGGCGGGTTTATCATTGAACATTTGCAGCATCCCCTGATACTGCGCCACCTGTTCTTCTTCCGa                                >  1:81752/1‑100 (MQ=255)

                                                           ctgctTATCTGCTCCGACATCCAGCGTACGCAAGGTGGCGGGTTTATCATTGAACATTTGCAGCATCCCCTGATACTGCGCCACCTGTTCTTCTTCCGAc                               <  2:1712726/100‑1 (MQ=255)

                                                             gctTATCTGCTCCGACATCCAGCGTACGCAAGGTGGCGGGTTTATCATTGAACATTTGCAGCATCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACgg                             >  1:30348/1‑100 (MQ=255)

                                                              ctTATCTGCTCCGACATCCAGCGTACGCAAGGTGGCGGGTTTATCATTGAACATTTGCAGCATCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACggg                            <  2:677569/100‑1 (MQ=255)

                                                               ttATCTGCTCCGACATCCAGCGTACGCAAGGTGGCGGGTTTATCATTGAACATTTGCAGCATCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGGa                           <  1:58654/100‑1 (MQ=255)

                                                                 aTCTGCTCCGACATCCAGCGTCCGCAAGGTGGCGGGTGTATCATTGAACATTTGCAGCATCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGGa                           <  1:3083646/98‑1 (MQ=255)

                                                                   cTGCTCCGACACCCAGCGTACGCAAGGTGGCGGGTTTATCATTGAACATTTGCAGCATCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGGAAAcc                       >  1:3105217/1‑100 (MQ=255)

                                                                    tGCTCCGACATCCAGCGTACGCAAGGTGGCGGGTTTATCATTGAACATTTGCAGCATCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGGAAACCa                      >  1:3263290/1‑100 (MQ=255)

                                                                     gCTCCGACATCCAGCGTACGCAAGGTGGCGGGTTTATCATTGAACATTTGCAGCATCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGGAAACCAc                     >  2:109708/1‑100 (MQ=255)

                                                                     gCTCCGACATCCAGCGTACGCAAGGTGGCGGGTTTATCATTGAACATTTGCAGCATCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGGAAACCAc                     >  1:600743/1‑100 (MQ=255)

                                                                       tCCGACATCCAGCGTACGCAAGGTGGCGGGTTTATCATTGAACATTTGCAGCATCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGGAAACCACtt                   >  2:1233884/1‑100 (MQ=255)

                                                                        ccGACATGCAGCGTACGCAAGGTGGCGGGTTTATCATTGAACATTTGCAGCATCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGGAAACCACttt                  >  2:1853794/1‑100 (MQ=255)

                                                                        ccGACATCCAGCGTACGCAAGGTGGCGGGTTTATCATTGAACATTTGCAGCATCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGGAAACCACttt                  >  2:1412591/1‑100 (MQ=255)

                                                                          gACATCCAGCGTACGCAAGGTGGCGGGTTTATCATTGAACATTTGCAGCATCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGGAAACCACTTTgc                <  2:1446124/100‑1 (MQ=255)

                                                                           aCATCCAGCGTACGCAAGGTGGCGGGTTTATCATTGAACATTTGCAGCATCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGGAAACCACTTTgca               <  2:2737490/100‑1 (MQ=255)

                                                                            cATCCAGCGTACGCAAGGTGGCGGGTTTATCATTGAACATTTGCAGCATCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGGAAACCACTTTgcag              <  2:2101458/100‑1 (MQ=255)

                                                                            cATCCAGCGTACGCAAGGTGGCGGGTTTATCATTGAACATTTGCAGCATCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGGAAACCACTTTgcag              >  1:180680/1‑100 (MQ=255)

                                                                            cATCCAGCGTACGCAAGGTGGCGGGTTTATCATTGAACATTTGCAGCATCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGGAAACCACTTTgcag              <  1:709137/100‑1 (MQ=255)

                                                                            cATCCAGCGTACGCAAGGTGGCGGGTTTATCATTGAACATTTGCAGCATCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGGAAACCACTTTgcag              <  1:2716117/100‑1 (MQ=255)

                                                                              tCCAGCGTACGCAAGGTGGCGGGTTTATCATTGAACATTTGCAGCATCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGGAAACCACTTTgcagca            >  1:3095672/1‑100 (MQ=255)

                                                                              tCCAGCGTACGCAAGGTGGCGGGTTTATCATTGAACATTTGCAGCATCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGGAAACCACTTTgcagca            >  1:1694511/1‑100 (MQ=255)

                                                                               ccAGCGTACGCAAGGTGGCGGGTTTATCATTGAACATTTGCAGCATCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGGAAACCACTTTGCAGCAt           <  1:1471251/100‑1 (MQ=255)

                                                                                    gTACGCAAGGTGGCGGGTTTATCATTGAACATTTGCAGCATCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGGAAACCACTTTGCAGCATGAATg      <  2:2209828/100‑1 (MQ=255)

                                                                                    gTACGCAAGGTGGCGGGTTTATCATTGAACATTTGCAGCATCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGGAAACCACTTTGCAGCATGAATg      <  2:2902676/100‑1 (MQ=255)

                                                                                    gTACGCAAGGTGGCGGGTTTATCATTGAACATTTGCAGCATCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGGAAACCACTTTGCAGCATGAATg      <  1:292419/100‑1 (MQ=255)

                                                                                      aCGCAAGGTGGCGGGTTTATCATTGAACATTTGCAGCATCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGGAAACCACTTTGCAGCATGAATggg    >  1:2544179/1‑100 (MQ=255)

                                                                                       cGCAAGGTGGCGGGTTTATCATTGAACATTTGCAGCATCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACAGGAAACCACTTTGCAGCATGAATGGGa   <  2:1704051/100‑1 (MQ=255)

                                                                                        gCAAGGTGGCGGGTTTATCATTGAACATTTGCAGCATCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGGAAACCACTTTGCAGCATGAATGGGAt  <  1:848632/100‑1 (MQ=255)

ATGCGAATCCCACGCCAACCCAGGCATGGATTCTCTTCGCTGATCGGCATGTAAGGCAGCTGCTTATCTGCTCCGACATCCAGCGTACGCAAGGTGACGGGTTTATCATTGAACATTTGCAGCATCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGGAAACCACTTTGCAGCATGAATGGGAT  >  NC_000913/2966855‑2967042

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`