Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 922,316:1 +C 18.8% intergenic (+23/+50) macB → / ← cspD macrolide ABC transporter peremase/ATPase/inhibitor of DNA replication, cold shock protein homolog

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913922,3141.C18.8% 302.9 / 79.7 96intergenic (+21/+52)macB/cspDmacrolide ABC transporter peremase/ATPase/inhibitor of DNA replication, cold shock protein homolog
Reads supporting (aligned to +/- strand):  ref base . (40/38);  new base C (6/12);  total (46/50)
Fisher's exact test for biased strand distribution p-value = 1.99e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.07e-01

Only 100 of 102 total aligned reads displayed.

CACCGGGATTTTATTTGGCTGGTTACCCGCACGAAATGCGGCACGACTGGATCCAGTAGATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAGCCGATCGGGCTGGCATTTTGCCTTTAGGATGTACACAATGAGACAGAAGAGCTATGCGACTGCCGCTTCTACTTCGACGGGCACAATAACACTGGCG  >  NC_000913/922215‑922411
                                                                                                    |                                                                                                 
  ccGGGATTTTATTTGGCTGGTTACCCGCACGAAATGCGGCACGACTGGATCCAGTAGATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAGcc                                                                                                 >  1:2744790/1‑100 (MQ=255)
  ccGGGATTTTATTTGGCTGGTTACCCGCACGAAATGCGGCACGACTGGATCCAGTAGATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAGc                                                                                                  >  2:1020918/1‑99 (MQ=255)
   cGGGATTTTATTTGGCTGGTTACCCGCACGAAATGCGGCACGACTGGATCCAGTAGATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAGcc                                                                                                 <  2:151031/99‑1 (MQ=255)
    gtgATTTTATTTGGCTGATTACCCGCACGAAATGCGGCACGACTGGATCCAGTAGATGCTCTGGCACGAGAGTAATGTTTGAGATAAACATGCCAGcc                                                                                                 <  2:830498/96‑1 (MQ=255)
    gggATTTTATTTGGCTGGTTACCCGCAAGAAATGCGGCACGACTGGATCCAGTAGATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAGc                                                                                                  <  2:308345/97‑1 (MQ=255)
      gATTTTATTTGGCTGGTTACCCGCACGAAATGCGGCACGACTGGATCCAGTAGATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAGCCGATc                                                                                             >  1:3063422/1‑100 (MQ=255)
      gATTTTATTTGGCTGGTTACCCGCACGAAATGCGGCACGACTGGATCCAGTAGATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAGCCGATc                                                                                             <  2:3024045/100‑1 (MQ=255)
        ttttATTTGGCTGGTTACCCGCACGAAATGCGGCACGACTGGATCCAGTAGATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAGCCCGATCg                                                                                            <  2:496160/100‑1 (MQ=255)
        ttttATTTGGCTGGTTACCCGCACGAAATGCGGCACGACTGGATCCAGTAGATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAGCCGATCgg                                                                                           >  2:2467989/1‑100 (MQ=255)
          ttATTTGGCTGGTTACCCGCACGAAATGCGGCACGACTGGATCCAGTAGATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAGCCGATCGGGc                                                                                         >  1:1298732/1‑100 (MQ=255)
          ttATTTGGCTGGTTACCCGCACGAAATGCGGCACGACTGGATCCAGTAGATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAGCCGATCGGGc                                                                                         >  2:2659351/1‑100 (MQ=255)
            aTTTGGCTTGTTACCCGCAAGAAATGCGGCACGACTGTATCCAGTAGATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAGCCGATCGGGCtc                                                                                       <  2:218969/100‑2 (MQ=255)
            aTTTGGCTGGTTACCCGCACGAAATGCGGCACCACTGGAGCCCGTAGATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAGCCGATCGGGCTg                                                                                       <  2:2181267/100‑1 (MQ=255)
             tttGGCTGGTTACCCGCACGAAATGCGGCACGACTGGATCCAGTAGATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAGCCGATCGGGCTgg                                                                                      <  1:2788514/100‑1 (MQ=255)
             tttGGCTGGTTACCCGCACGAAATGCGGCACGACTGGATCCAGTAGATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAGCCGATCGGGCTgg                                                                                      <  2:1151033/100‑1 (MQ=255)
               tGGCTGGTTACCCGCACGAAATGCGGCACGACTGGATCCAGTAGATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAGCCGATCGGGCTGGCa                                                                                    <  2:1254320/100‑1 (MQ=255)
                ggCTGGTTACCCGCACGAAATGCGGCACGACTGGATCCAGTAGATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAGCCGATCGGGCTGGCAt                                                                                   <  2:1280561/100‑1 (MQ=255)
                 gCTGGTTACCCGCACGAAATGCGGCACGACTGGATCCAGTAGATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAGCCCGATCGGCTGGCAtt                                                                                  <  2:2538489/100‑1 (MQ=39)
                 gCTGGTTACCCGCACGAAATGCGGCACGACTGGATCCAGTAGATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAGCCGATCGGGCTGGCAtt                                                                                  >  1:2846447/1‑100 (MQ=255)
                  cTGGTTACCCGCACGAAATGCGGCACGACTGGATCCAGTAGATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAGCCGATCGGGCTGGCAttt                                                                                 >  2:2894774/1‑100 (MQ=255)
                  cTGGTTACCCGCACGAAATGCGGCACGACTGGATCCAGTAGATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAGCCGATCGGGCTGGCAttt                                                                                 <  2:2222012/100‑1 (MQ=255)
                  cTGGTTACCCGCACGAAATGCGGCACGACTGGATCCAGTAGATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAGCCGATCGGGCTGGCAttt                                                                                 >  1:118659/1‑100 (MQ=255)
                   cgatctcccGCACGAAATGCGGCACGACTGGATCCAGTAGATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAGCCGATCGGGCTGGCAtttt                                                                                <  2:13699/94‑1 (MQ=255)
                    ggTTACCCGCACGAAATGCGGCACGACTGGATCCAGTAGATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAGCCCGATCGGCTGGCAttttt                                                                               <  1:165551/100‑2 (MQ=38)
                    ggTTACCCGCACGAAATGCGGCACGACTGGATCCAGTAGATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAGCCGATCGGGCTGGCATTTTg                                                                               <  2:2744762/100‑1 (MQ=255)
                    ggTTACCCGCACGAAATGCGGCACGACTGGATCCAGTAGATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAGCCGATCGGGCTGGCATTTTg                                                                               >  2:2988020/1‑100 (MQ=255)
                     gTTACCCGCACGAAATGCGGCACGACTGGATCCAGTAGATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAGCCGATCGGGCTGGCATTTTGc                                                                              >  1:1987622/1‑100 (MQ=255)
                     gTTACCCGCACGAAATGCGGCACGACTGGATCCAGTAGATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAGCCGATCGGGCTGGCATTTTGc                                                                              >  1:1985467/1‑100 (MQ=255)
                     gTTACCCGCACGAAATGCGGCACGACTGGATCCAGTAGATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAGCCGATCGGGCTGGCATTTTGc                                                                              <  2:1626333/100‑1 (MQ=255)
                      ttACCCGCACGAAATGCGGCACGACTGGATCCAGTAGATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAGCCCGATCGGCTGGCAtttttat                                                                             <  1:2415432/100‑4 (MQ=38)
                      ttACCCGCACGAAATGCGGCACGACTGGATCCAGTAGATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAGCCCGATCGGCTGGCAtttttat                                                                             <  1:2415339/100‑4 (MQ=38)
                      ttACCCGCACGAAATGCGACACTACTGGATCCAGTAGATGCTCTGGCACGAGAGTAATTTTTGATTTAAAAATGCCATCCGATCGGGCTGGCATTTTg                                                                               <  2:1341564/98‑1 (MQ=255)
                       tACCCGCACGAAATGCGGCACGACTGGATCCAGTAGATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAGCCCGATCGGCTGGCAtttttatc                                                                            <  1:2446837/100‑5 (MQ=38)
                       tACCCGCACGAAATGCGGCACGACTGGATCCAGTAGATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAGCCCGATCGGCTGGCAtttttatc                                                                            <  1:555214/100‑5 (MQ=38)
                        aCCCGCACGAAATGCGGCACGACTGGATCCAGTAGATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAGCCCGATCGGCTGGCAtttttatct                                                                           <  1:1761834/100‑6 (MQ=37)
                         cccGCACGAAATGCGGCACGACTGGATCCAGTAGATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAGCCCGATCGGCTGGCAtttttatctc                                                                          <  1:687397/100‑7 (MQ=37)
                         cccGCACGAAATGCGGCACGACTGGATCCAGTAGATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAGCCGATCGGGCTGGCAttttagatcg                                                                          >  1:13699/1‑94 (MQ=255)
                         cccGCACGAAATGCGGCACGACTGGATCCAGTAGATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAGCCGATCGGGCTGGCATTTTGCCttt                                                                          >  1:1355631/1‑100 (MQ=255)
                         cccGCACGAAATGCGGCACGACTGGATCCAGTAGATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAGCCGATCGGGCTGGCATTTTGCCttt                                                                          >  1:1784999/1‑100 (MQ=255)
                         cccGCACGAAATGCGGCACGACTGGATCCAGTAGATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAGCCGATCGGGCTGGCATTTTGCCtt                                                                           <  2:1691601/99‑1 (MQ=255)
                          ccGCACGAAATGCGGCACGACTGGATCCAGTAGATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAGCCCGATCGGCTGGCAtttttatctca                                                                         <  1:938458/100‑8 (MQ=37)
                          ccGCACGAAACGCGGCACGACTGGATCCAGTAGATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAGCCCGATCGGCTGGCAtttgtatctc                                                                          <  2:328798/99‑8 (MQ=37)
                            gCACGAAATGCGGCACGACTGGATCCAGTAGATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAGCCGATCGGGCTGGCATTTTGCCTTTAgg                                                                       <  1:201614/100‑1 (MQ=255)
                             cACGAAATGCGGCACGACTGGATCCAGTAGATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAGCCGATCGGGCTGGCATTTTGCCTTTAGGa                                                                      <  2:2988611/100‑1 (MQ=255)
                                gcaaTGCGGCACGACTGGATCCAGTAGATGCTTTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAGCCGATCGGGCTGGCATTTTGCCTTTAGGATGt                                                                   <  2:800371/98‑1 (MQ=255)
                                   aTGCGGCACGACTGGATCCAGTAGATGCTCTGGCACCAGAGTAATTTTTTTTTTTTTTTTTCCAGCCGATCGGGCTGGCATTTTTCCTTTAGTAt                                                                     >  2:32840/1‑95 (MQ=255)
                                    tGCGGCACGACTGGATCCAGTAGATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAGCCGATCGGGCTGGCATTTTGCCTTTAGGATGTacac                                                               >  1:675957/1‑100 (MQ=255)
                                      cGGCACGACTGGATCCAGTAGATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAGCCGATCGGGCTGGCATTTTGCCTTTAGGATGTACACaa                                                             >  2:567136/1‑100 (MQ=255)
                                        gCACGACTGGATCCAGTAGATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAGCCGATCGGGCTGGCATTTTGCCTTTAGGATGTACACAATg                                                           <  1:2567376/100‑1 (MQ=255)
                                           cGACTGGATCCAGTAGATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAGCCGATCGGGCTGGCATTTTGCCTTTAGGATGTACACAATgaga                                                        <  2:438354/100‑1 (MQ=255)
                                           cGACTGGATCCAGTAGATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAGCCGATCGGGCTGGCATTTTGCCTTTAGGATGTACACAATgaga                                                        <  1:2894803/100‑1 (MQ=255)
                                           cGACTGGATCCAGTAGATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAGCCGATCGGGCTGGCATTTTGCCTTTAGGATGTACACAATgaga                                                        >  1:3086756/1‑100 (MQ=255)
                                            ggcgataTCCAGTAGATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAGCCGATCGGGCTGGCATTTTGCCTTTAGGATGTACACAATGAGAc                                                       <  2:2325750/94‑1 (MQ=255)
                                            gACTGGATCCAGTAGATGCTCTGGCACGAGAGTAATTTTTGAGAGAAAAATGCCAGCCGATCGGGCTGGCATTTTGCCTTTAGGATGTACACAATGAGAc                                                       <  1:805576/100‑1 (MQ=255)
                                              cTGGATACAGTAGATGCTCTGGCACGAGAGTAATTTTTGAGATACAAATGCCAGCCGATCGGCCTGGCGTTGTCCCGTCAGGATGAACACACGGAGTCag                                                     >  2:2604028/1‑100 (MQ=255)
                                               tGGATCCAGTAGATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAGCCGATCGGGCTGGCATTTTGCCTTTAGGATGTACACAATGAGACaga                                                    <  2:30094/100‑1 (MQ=255)
                                               gatctCCAGTAGATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAGCCGATCGGGCTGGCATTTTGCCTTTAGGATGTACACAATGAGACaga                                                    <  2:1518443/96‑1 (MQ=255)
                                                ggATCCAGTAGATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAGCCGATCGGGCTGGCATTTTGCCTTTAGGATGTACACAATGAGACagaa                                                   >  1:1646732/1‑100 (MQ=255)
                                                 gATCCAGTAGATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAGCCGATCGGGCTGGCATTTTGCCTTTAGGATGTACACAATGAGACagaag                                                  >  2:120365/1‑100 (MQ=255)
                                                  aTCCAGTAGATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAGCCGATCGGGCTGGCATTTTGCCTTTAGGATGTACACAATGAGACagaaga                                                 >  1:214991/1‑100 (MQ=255)
                                                  aTCCAGTAGATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAGCCGATCGGGCTGGCATTTTGCCTTTAGGATGTACACAATGAGACagaaga                                                 >  1:1967170/1‑100 (MQ=255)
                                                    ccAGTAGATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAGCCGATCGGGCTGGCATTTTGCCTTTAGGATGTACACAATGAGACagaagatc                                               >  1:1518454/1‑98 (MQ=255)
                                                     cAGTAGATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAGCCGATCGGGCTGGCATTTTGCCTTTAGGATGTACACAATGAGACAGAAGAGCt                                              >  1:443431/1‑100 (MQ=255)
                                                     cAGTAGATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAGCCGATCGGGCTGGCATTTTGCCTTTAGGATGTACACAATGAGACAGAAGAGCt                                              >  2:220713/1‑100 (MQ=255)
                                                      aGTAGATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAGCCGATCGGGCTGGCATTTTGCCTTTAGGATGTACACAATGAGACAGAAGAGCTa                                             >  1:1734110/1‑100 (MQ=255)
                                                          gATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAGCCGATCGGGCTGGCATTTTGCCTTTAGGATGTACACAATGAGACAGAAGAGCTATGCg                                         <  1:2486039/100‑1 (MQ=255)
                                                            tGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAGCCGATCGGGCTGGCATTTTGCCTGTAGGATGTACACAATGAGACAGAAGAGCTATGCGAc                                       >  2:919489/1‑100 (MQ=255)
                                                              ctctGGCACGAGAGTAATTTTTGAGATAAAAATGCCAGCCGATCGGGCTGGCATTTTGCCTTTAGGATGTACACAATGAGACAGAAGAGCTATGCGACTg                                     <  2:1552828/100‑1 (MQ=255)
                                                                ctGGCACGAGTGTAATTTTTGAGATAAAAATGCCAGACGATCGGGCTGGCATTTTGCCTTTAGGATGTACACAATGAGACAGAAGATCTATGCGACTg                                     <  2:2387255/98‑1 (MQ=255)
                                                                ctGGCACGAGAGTAATTTTTGAGATAAAAATGCCAGCCGATCGGGCTGGCATTTTGCCTTTAGGATGTACACAATGAGACAGAAGAGCTATGCGACTGcc                                   <  1:2871123/100‑1 (MQ=255)
                                                                ctGGCACGAGAGTAATTTTTGAGATAAAAATGCCAGCCGATCGGGCTGGCATTTTGCCTTTAGGATGTACACAATGAGACAGAAGAGCTATGCGACTGcc                                   >  1:1510595/1‑100 (MQ=255)
                                                                    cACGAGAGTAATTTTTGAGATAAAAATGCCAGCCGATCGGGCTGGCATTTTGCCTTTAGGATGTACACAATGAGACAGAAGAGCTATGCGACTGCCGCtt                               >  1:1755748/1‑100 (MQ=255)
                                                                    cACGAGAGTAATTTTTGAGATAAAAATGCCAGCCGATCGGGCTGGCATTTTGCCTTTAGGATGTACACAATGAGACAGAAGAGCTATGCGACTGCCGCtt                               >  1:1686019/1‑100 (MQ=255)
                                                                      cGAGAGTAATTTTTGAGATAAAAATGCCAGCCGATCGGGCTGGCATTTTGCCTTTAGGATGTACACAATGAGACAGAAGAGCTATGCGACTGCCGCTTCt                             <  2:2638944/100‑1 (MQ=255)
                                                                       gagaGTAATTTTTGAGATAAAAATGCCAGCCGATCGGGCTGGCATTTTGCCTTTAGGATGTACACAATGAGACAGAAGAGCTATGCGACTGCCGCTTCt                             <  2:2964237/99‑1 (MQ=255)
                                                                       gagaGTAATTTTTGAGATAAAAATGCCAGCCGATCGGGCTGGCATTTTGCCTTTAGGATGTACACAATGAGACAGAAGAGCTATGCGACTGCCGCTTCTa                            <  1:2988049/100‑1 (MQ=255)
                                                                        agagTAATTTTTGAGATAAAAATGCCAGCCGATCGGGCTGGCATTTTGCCTTTAGGATGTACACAATGAGACAGAAGAGCTATGCGACTGCCGCTTCTAc                           <  1:1103808/100‑1 (MQ=255)
                                                                          agTAATTTTTGAGATAAAAATGCTAGGAGATCGGGCTGGCATTTTGCCAATAGGACGTACAGAAGGAGGCAGAAGAGGTATGCGACTGCAGCTTCTAGtt                         >  2:2944647/1‑100 (MQ=255)
                                                                          agTAATTTTTGAGATAAAAATGCCAGCCGATCGGGCTGGCATTTTGCCTTTAGGATGTACACAATGAGACAGAAGAGCTATGCGACTGCCGCTTCTACtt                         >  1:957347/1‑100 (MQ=255)
                                                                              aTTTTTGAGATAAAAATGCCAGCCGATCGGGCTGGCATTTTGCCTTTAGGATGTACACAATGAGATAGAAGAGCTATCCGGCTGCCGCTTCTACTTCg                       >  2:2542949/1‑98 (MQ=255)
                                                                              aTTTTTGAGATAAAAATGCCAGCCGATCGGGCTGGCATTTTGCCTTTAGGATGTACACAATGAGACAGAAGAGCTATGCGACTGCCGCTTCTACTTCGAc                     >  1:2515917/1‑100 (MQ=255)
                                                                               tttttGAGATAAAAATGCCAGCCGATCGGTCTGGCATTTTGCCTTTAGGATGTACACAATGATACAGAATATCTATGCGACTGCCGCTTCTACTTCGAcn                    <  2:1116230/100‑2 (MQ=255)
                                                                                ttttGAGATAAAAATGCCAGCCGATCGGGCTGGCATTTTGCCTTTAGGATGTACACAATGAGACAGAAGAGCTATGCGACTGCCGCTTCTACTTCGACgg                   >  2:1452708/1‑100 (MQ=255)
                                                                                      gaTAAAAATGCCAGCCGATCGGGCTGGCATTTTGCCTTTAGGATGTACACAATGAGACAGAAGAGCTATGCGACTGCCGCTTCTACTTCGACGGGCACaa             >  1:3077966/1‑100 (MQ=255)
                                                                                       ggcaaaaTGCCAGCCCGATCGGCTGGCATTTTGCCTTTAGGATGTACAAAATGAGACAGAAGAGCTATGCGACTGCCGCTTCTACTTCGACGGGCACAAt            >  2:1889911/4‑100 (MQ=37)
                                                                                       cgatctaTGCCAGCCCGATCGGCTGGCATTTTGCCTTTAGGATGTACACAATGAGACAGAAGAGCTATGCGACTGCCGCTTCTACTTCGACGGGCACAAt            <  2:1254726/94‑1 (MQ=38)
                                                                                       aTAAAAATGCCAGCCGATCGGGCTGGCATTTTGCCTTTAGGATGTACACAATGAGACAGAAGAGCTATGCGACTGCCGCTTCTACTTCGACGGGCACAAt            <  1:1004499/100‑1 (MQ=255)
                                                                                        tAAAAATGCCAGCCGATCGGGCTGGCATTTTGCCTTTAGGATGTACACAATGAGACAGAAGAGCTATGCGACTGCCGCTTCTACTTCGACGGGCACAATa           <  1:2468014/100‑1 (MQ=255)
                                                                                         aaaaaTGCCAGCCGATCGGGCTGGCATTTTGCCTTTAGGATGTACACAATGAGACAGAAGAGCTATGCGACTGCCGCTTCTACTTCGACGGGCACAATaa          >  2:2892609/1‑100 (MQ=255)
                                                                                            aaTGCCAGCCCGATCGGCTGGCATTTTGCCTTTAGGATGTACACAATGAGACAGAAGAGCTATGCGACTGCCGCTTCTACTTCGACGGGCACAATAacac       >  1:2839953/1‑100 (MQ=39)
                                                                                             aTGCCAGCCCGATCGGCTGGCATTTTGCCTTTAGGATGTACACAATGAGACAGAAGAGCTATGCGACTGCCGCTTCTACTTCGACGGGCACAATagatcg      >  1:1254735/1‑95 (MQ=38)
                                                                                             aTGCCAGCCCGATCGGCTGGCATTTTGCCTTTAGGATGTACACAATGAGACAGAAGAGCTATGCGACTGCCGCTTCTACTTCGACGGGCACAATAACACt      >  1:2881107/1‑100 (MQ=39)
                                                                                             aTGCCAGCCCGATCGGCTGGCATTTTGCCTTTAGGATGTACACAATGAGACAGAAGAGCTATGCGACTGCCGCTTCTACTTCGACGGGCACAATAACACt      >  1:1240706/1‑100 (MQ=39)
                                                                                             aTGCCAGCCGATCGGGCTGGCATTTTGCCTTTAGGATGTCCACAATGAGACAGAAGAGCTATGCGACTGCCGCTTCTACTTCGACGGGCACAATAACACt      >  2:2970714/1‑100 (MQ=255)
                                                                                             aTGCCAGCCGATCGGGCTGGCATTTTGCCTTTAGGATGTACACAATGAGACAGAAGAGCTATGCGACTGCCGCTTCTACTTCGACGGGCACAATAACACt      <  1:405657/100‑1 (MQ=255)
                                                                                             aTGCCAGCCGATCGGGCTGGCATTTTGCCTTTAGGATGTACACAATGAGACAGAAGAGCTATGCGACTGCCGCTTCTACTTCGACGGGCACAATAACACt      <  1:1433685/100‑1 (MQ=255)
                                                                                              tGCCAGCCCGATCGGCTGGCATTTTGCCTTTAGGATGTACACAATGAGACAGAAGAGCTATGCGACTGCCGCTTCTACTTCGACGGGCACAATAACACTg     >  1:78889/1‑100 (MQ=255)
                                                                                               gCCAGCCGATCGGGCTGGCATTTTGCCTTTAGGATGTACACAATGAGACAGAAGAGCTATGCGACTGCCGCTTCTACTTCGACGGGCACAATAACACTgg    <  2:1748808/100‑1 (MQ=255)
                                                                                               gCCAGCCGATCGGGCTGGCATTTTGCCTTTAGGATGTACACAATGAGACAGAAGAGCTATGCGACTGCCGCTTCTACTTCGACGGGCACAATAACACTgg    >  2:2126627/1‑100 (MQ=255)
                                                                                                  aGCCGATCGGGCTGGCATTTTGCCTTTAGGATGTACACAATGAGACAGAAGAGCTATGCGACTGCCGCTTCTACTTCGACGGGCACAATAACACGGGCg  >  2:1788517/1‑99 (MQ=255)
                                                                                                    |                                                                                                 
CACCGGGATTTTATTTGGCTGGTTACCCGCACGAAATGCGGCACGACTGGATCCAGTAGATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAGCCGATCGGGCTGGCATTTTGCCTTTAGGATGTACACAATGAGACAGAAGAGCTATGCGACTGCCGCTTCTACTTCGACGGGCACAATAACACTGGCG  >  NC_000913/922215‑922411

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: