Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,248,432:1 (G)5→6 100% coding (660/663 nt) yqjA → general envelope maintenance protein; DedA family inner membrane protein; putative multidrug efflux transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,248,4271.G71.3% 236.0 / 107.9 94coding (655/663 nt)yqjAgeneral envelope maintenance protein; DedA family inner membrane protein; putative multidrug efflux transporter
Reads supporting (aligned to +/- strand):  ref base . (16/11);  new base G (44/23);  total (60/34)
Fisher's exact test for biased strand distribution p-value = 6.37e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.44e-01

Only 100 of 106 total aligned reads displayed.

CCAGCTGATGTCATGCCTGATGCTGCTCCCGGTGGTGCTGCTGGTGTTTGGCCTGGCAGGTTCTCTGGTCGTGTTATGGAAAAAGAAATATGGAAATCGGGGGTAAGGGATGCAAATACCTCGCATGTCGCTTCGCCAGCTAGCCTGGTCCGGCGCTGTTTTACTTCTGGTCGGCACGCTGTTGCTGGCCTGG  >  NC_000913/3248330‑3248522
                                                                                                  |                                                                                                 
ccAGCTGATGTCATGCCTGATGCTGCTCCCGGTGGTGCTGCTGGTGTTTGGCCTGGCAGGTTCTCTGGTCGTGTTATGGAAAAAGAAATATGGAAATCgg                                                                                                 >  1:618733/1‑100 (MQ=255)
ccAGCTGATGTCATGCCTGATGCTGCTCCCGGTGGTGCTGCTGGTGTTTGGCCTGGCAGGTTCTCTGGTCGTGTTATGGAAAAAGAAATATGGAAATCgg                                                                                                 >  2:2131734/1‑100 (MQ=255)
 cAGCTGATGTCATGCCTGATGCTGCTCCCGGTGGTGCTGCTGGTGTTTGGCCTGGCAGGTTCTCTGGTCGTGTTATGGAAAAAGAAATATGGAAATCggg                                                                                                >  1:1092559/1‑100 (MQ=255)
  ctcTGATGTCATGCCTGATGCTGCTCCCGGTGGTGCTGCTGGTGTTTGGCCTGGCAGGTTCTCTGGTCGTGTTATGGAAAAAGAAATATGGAAATCgggg                                                                                               <  1:153776/98‑1 (MQ=255)
  atcTGATGTCATGCCTGATGCTGCTCCCGGTGGTGCTGCTGGTGTTTGGCCTGGCAGGTTCTCTGGTCGTGTTATGGAAAAAGAAATATGGAAATCgggg                                                                                               <  1:631772/98‑1 (MQ=255)
   gcggATGTCATGCCTGATGTTGCTCCCGGTGGTGCTGCTGGTGTTTGGCCTGGCAGGGTCTCTGGTCGTTTTATGGAAAAAGAAATATGGAAATCggcg                                                                                               <  2:1112015/96‑3 (MQ=255)
   gCTGGTGTCATGCCTGATGCTGCTCCCGGTGGTGCTGCTGTAGTTTGGCCTGGCAGGTTCTCTGGTCGTGTTATGGAAAAAGAAATATGGAAATCgggg                                                                                               <  2:2921713/99‑1 (MQ=255)
   gCTGATGTCATGTCTGATGCTGCTCCCGGTGGTGCTGCTGGTGTTTGGCCTGGCAGGTTCTCTGGTCGTGTTATGGAAAAAGAAATATGGAAATCggggg                                                                                             <  2:2541820/100‑1 (MQ=255)
   gCTGATGTCATGCCTGATGCTGCTCCCGGTGGTGCTGCTGGTGTTTGGCCTGGCAGGTTCTCTGGTCGTGTTATGGAAAAAGAAATATGGAAATCggggg                                                                                             >  1:790103/1‑100 (MQ=255)
   gCTGATGTCATGCCTGATGCTGCTCCCGGTGGTGCTGCTGGTGTTTGGCCTGGCAGGTTCTCTGGTCGTGTTATGGAAAAAGAAATATGGAAATCggggg                                                                                             <  1:2939586/100‑1 (MQ=255)
    cTGATGTCATGCCTGATGCTGCTCCCGGTGGTGCTGCTGGTGTTTGGCCTGGCAGGTTCTCTGGTCGTGTTATGGAAAAAGAAATATGGAAATCggggag                                                                                             >  2:153774/1‑98 (MQ=255)
     tGATGTCATGCCTGATGCTGCTCCCGGTGGTGCTGCTGGTGTTTGGCCTGGCAGGTTCTCTGGTCGTGTTATGGAAAAAGAAATATGGAAATCGGGGGTa                                                                                          <  1:53479/100‑1 (MQ=255)
         gTCATGCCTGATGCTGCTCCCGGTGGTGCTGCTGGTGTTTGGCCTGGCAGGTTCTCTGGTCGTGTTATGGAAAAAGAAATATGGAAATCGGGGGGTAAgg                                                                                       <  1:2766014/100‑1 (MQ=255)
         gTCATGCCTGATGCTGCTCCCGGTGGTGCTGCTGGTGTTTGGCCTGGCAGGTTCTCTGGTCGTGTTATGGAAAAAGAAATATGGAAATCGGGGGGTAAgg                                                                                       >  1:992103/1‑100 (MQ=255)
          tCATGCCTGATGCTGCTCCCGGTGGTGCTGCTGGTGTTTGGCCTGGCAGGTTCTCTGGTCGTGTTATGGAAAAAGAAATATGGAAATCGGGGGGTAAggg                                                                                      >  2:2506804/1‑100 (MQ=255)
          tCATGCCTGATGCTGCTCCCGGTGGTGCTGCTGGTGTTTGGCCTGGCAGGTTCTCTGGTCGTGTTATGGAAAAAGAAATATGGAAATCGGGGGGTAAggg                                                                                      <  1:1902755/100‑1 (MQ=255)
           cATGCCTGATGCTGCTCCCGGTGGTGCTGCTGGTGTTTGGCCTGGCAGGTTCTCTGGTCGTGTTATGGAAAAAGAAATATGGAAATCGGGGGGTAAGGGa                                                                                     >  2:1217712/1‑100 (MQ=255)
           cATGCCTGATGCTGCTCCCGGTGGTGCTGCTGGTGTTTGGCCTGGCAGGTTCTCTGGTCGTGTTATGGAAAAAGAAATATGGAAATCGGGGGTAAGGGAt                                                                                    >  2:524272/1‑100 (MQ=255)
           cATGCCTGATGCTGCTCCCGGTGGTGCTGCTGGTGTTTGGCCTGGCAGGTTCTCTGGTCGTGTTATGGAAAAAGAAATATGGAAATCGGGGGTAAGGGAt                                                                                    >  1:104254/1‑100 (MQ=255)
            aggcgtGATGCGGCTCTCGGTGGTGCTGCTGGTGTGTGGCCTGGCAGGTTCTCTGGTCGTGTTATGGAAAAAGAAATATGGAAATCGGGGGGTAAggg                                                                                      <  2:2528647/93‑1 (MQ=255)
             tGCCTGGTGCTGCTCCTGGTGGTGCTGATGGTGTTTGGCCTGGCAGGTTCTCTGGTCGTGATATGGAAAAAGAAATATGGAAATCGGGGGGTAAgg                                                                                       <  2:1133183/96‑1 (MQ=255)
               ccTGATGCTGCTCCCGGTGGTGCTGCTGGTGTTTGGCCTGGCAGGTTCTCTGGTCGTGTTATGGAAAAAGAAATATGGAAATCGGGGTGTAAGGGATGCa                                                                                 <  1:2048360/100‑1 (MQ=255)
                 tGATGCTGCTCCCGGTGGTGCTGCTGGTGTTTGGCCTGGCAGGGTCTCTGGTCGTGTTATGGAAAAAGAAATATGGAAATCGGGGGTAAGGGATGCa                                                                                 <  2:594811/97‑1 (MQ=255)
                    tgctgcTCCCGGTGGTGCTGCTGGTGTTTGGCCTGGCAGGTTCTCTGGTCGTGTTATGGAAAAAGAAATATGGAAATCGGGGGGTAAGGGATGCAAATAc                                                                            >  2:2439680/1‑100 (MQ=255)
                    tgctgcTCCCGGTGGTGCTGCTGGTGTTTGGCCTGGCAGGTTCTCTGGTCGTGTTATGGAAAAAGAAATATGGAAATCGGGGGGTAAGGGATGCAAATAc                                                                            <  1:858391/100‑1 (MQ=255)
                    tgctgcTCCCGGTGGTGCTGCTGGTGTTTGGCCTGGCAGGTTCTCTGGTCGTGTTATGGAAAAAGAAATATGGAAATCGGGGGGTAAGGGATGCAAATAc                                                                            >  1:39223/1‑100 (MQ=255)
                    tgctgcTCCCGGTGGTGCTGCTGGTGTTTGGCCTGGCAGGTTCTCTGGTCGTGTTATGGAAAAAGAAATATGGAAATCGGGGGTAAGGGATGCAAATAcc                                                                           >  2:1386241/1‑100 (MQ=255)
                       tgctCCCGGTGGTGCTGCTGGTGTTTGGCCTGGCAGGTTCTCTGGTCGTGTTATGGAAAAAGAAATATGGAAATCGGGGGGTAAGGGATGCAAATACCt                                                                          >  2:565438/1‑99 (MQ=255)
                       tgctCCCGGTGGTGCTGCTGGTGTTTGGCCTGGCAGGTTCTCTGGTCGTGTTATGGAAAAAGAAATATGGAAATCGGGGGGTAAGGGATGCAAATACCTc                                                                         >  1:880333/1‑100 (MQ=255)
                        gctCCCGGTGGTGCTGCTGGTGTTTGGCCTGGCAGGTTCTCTGGTCGTGTTATGGAAAAAGAAATATGGAAATCGGGGGGTAAGGGATGCAAATACCTCg                                                                        <  1:747803/100‑1 (MQ=255)
                         ctCCCGGTGGTGCTGCTGGTGTTTGGCCTGGCAGGTTCTCTGGTCGTGTTATGGAAAAAGAAATATGGAAATCGGGGGTAAGGGATGCAAATACCTCGCa                                                                      >  2:1024763/1‑100 (MQ=255)
                              ggtggtGCTGCTGGTGTTTGGCCTGGCAGGTTCTCGGGTCGTGTTATGGAAAAAGAAATATGGAAATCGGGGGGTAAGGGATGCAAATACCTCGCATGTc                                                                  <  1:1035522/100‑1 (MQ=255)
                                tggtgCTGCTGGTGTTTGGCCTGGCAGGTTCTCTGGTCGTGTTATGGAAAAAGAAATATGGAAATCGGGGGGTAAGGGATGCAAATACCTCGCATGTCGc                                                                >  1:2741243/1‑100 (MQ=255)
                                   tgctgcTGGTGTTTGGCCTGGCAGGTTCTCTGGTCGTGTTATGGAAAAAGAAATATGGAAATCGGGGGTAAGGGATGCAAATACCTCGCATGTCGCTTCg                                                            >  1:1117478/1‑100 (MQ=255)
                                    gctgctGGTGTTTGGCCTGGCAGGTTCTCTGGTCGTGTTATGGAAAAAGAAATATGGAAATCGGGGGGTAAGGGATGCAAATACCTCGCATGTCGCTTCg                                                            <  1:2585844/100‑1 (MQ=255)
                                    gctgctGGTGTTTGGCCTGGCAGGTTCTCTGGTCGTGTTATGGAAAAAGAAATATGGAAATCGGGGGGTAAGGGATGCAAATACCTCGCATGTCGCTTCg                                                            >  1:618873/1‑100 (MQ=255)
                                       gctgGTGTTTGGCCTGGCAGGTTCTCTGGTCGTGTTATGGAAAAAGAAATATGGAAATCGGGGGGTAAGGGATGCAAATACCTCGCATGTCGCTTAGCCa                                                         >  2:1112952/1‑100 (MQ=255)
                                        ctgGTGTTTGGCCTGGCAGGTTCTCTGGTCGTGTTATGGAAAAAGAAATATGGAAATCGGGGGGTAAGGGATGCAAATACCTCGCATGTCGCTTCGCCa                                                         >  2:1996048/1‑99 (MQ=255)
                                        ctgGTGTTTGGCCTGGCAGGTTCTCTGGTCGTATTATGGTAAGAGAAATATGGAAATCGGGGGGTAAGGGATGCAAATACCTCGCATGTCGCTTCGCCAg                                                        <  2:2182522/100‑1 (MQ=255)
                                        cgggTGTTTGGCCTGGCAGGTTCTCTGGTCGTGTTATGGAAAAAGAAATATGGAAATCGGGGGGTAAGGGATGCAAATACCTCGCATGTCGCTTCGCCAg                                                        >  1:2114564/3‑100 (MQ=255)
                                          ggTGTTTTGCCTGGCAGGTTCTCTGGTGGCGTTATGGAAAAAGAAATATGGAAATCGGGGGGTAAGGGATGCGAATACCTCGCATGTCGCTTCGCCAGc                                                       <  2:2893215/99‑1 (MQ=255)
                                          ggTGTTTGGCCCGGCAGGTTCTCTGGTCGTGTTATGGAAAAAGAAATATGGAAATCGGGGGGTAAGGGATGCAAATACCTCGCATGTCGCTTCGCCAGCt                                                      >  2:1434975/1‑100 (MQ=255)
                                           gtgtTTGGCCTGGCAGGTTCTCTGGTCGTGTTATGGAAAAAGAAATATGGAAATCGGGGGGTAAGGGATGCAAATACCTCGCATGTCGCTTCGCCAGCTa                                                     <  1:2864801/100‑1 (MQ=255)
                                           gtgtTTGGCCTGGCAGGTTCTCTGGTCGTGTTATGGAAAAAGAAATATGGAAATCGGGGGTAAGGGATGCAGATACCTCGCATGTCGCTTCGCCAGCTAg                                                    >  2:362314/1‑100 (MQ=255)
                                           gtgtTTGGCCTGGCAGGGTCTCTGGTCGTGTTATGGAAAAAGAAATATGGAAATCGGGGGGTAAGGGATGCAAATACCTCGCATGTCGCTTCGCCAGCTa                                                     <  2:2741215/100‑1 (MQ=255)
                                            tgtTTGGCCTGGCAGGTTCTCTGGTCGTGTTATGGAAAAAGAAATATGGAAATCGGGGGGTAAGGGATGCAAATACCTCGCATGTCGCTTCGCCAGCTAg                                                    >  1:474096/1‑100 (MQ=255)
                                             gtTTGGCCTGGCAGGTTCTCTGGTCGTGTTATGGAAAAAGAAATATGGAAATCGGGGGGTAAGGGATGCAAATACCTCGCATGTCGCTTCGCCAGCTAGc                                                   >  1:521388/1‑100 (MQ=255)
                                             gtTGGGGCTGGCAGGTTCTCTGGTCGTGTTATGGAAAAAGAAATATGGAAATCGGGGGTAAGGGATGCAAATACCTCGCATGTCGCTTCGCCAGCTAGcc                                                  <  2:784809/100‑1 (MQ=255)
                                              tgtGGCCTGGCAGGTTCTCTGGTCGTGTTATGGAAAAAGAAATATGGAAATCGGGGGGTAAGGGATGCAAATACCTCGCATGTCGCTTCGCCAGCTAGcc                                                  <  1:1609412/98‑1 (MQ=255)
                                                 ggCCTGGCAGGTTCTCTGGTCGTGTTATGGAAAAAGAAATATGGAAATCGGGGGGTAAGGGATGCAAATACCTCGCATGTCGCTTCGCCAGCTAGCCTgg                                               <  1:2262727/100‑1 (MQ=255)
                                                 ggCCTGGCAGGTTCTCTGGTCGTGTTATGGAAAAAGAAATATGGAAATCGGGGGGTAAGGGATGCAAATACCTCGCATGTCGCTTCGCCAGCTAGCCTgg                                               >  1:2651533/1‑100 (MQ=255)
                                                  gCCTGGCAGGTTCTCTGGTCGTGTTATGGAAAAAGAAATATGGAAATCGGGGGGTAAGGGATGCAAATACCTCGCATGTCGCTTCGCCAGCTAGCCTGGt                                              >  1:700993/1‑100 (MQ=255)
                                                  gCCTGGCAGGTTCTCTGGTCGTGTTATGGAAAAAGAAATATGGAAATCGGGGGGGAAGGGATGCAAATACCTCGCATGTCGCTTCGCGAGATAGcat                                                 >  2:2438504/1‑95 (MQ=255)
                                                   ccTGGCAGGTTCTCTGGTCGTGTTATGGAAAAAGAAATATGGAAATCGGGGGGTAAGGGATGCAAATACCTCGCATGTCGCTTCGCCAGCTAGCCTGGTc                                             >  1:1522324/1‑100 (MQ=255)
                                                    cTGGCAGGTTCTCTGGTCGTGTTATGGAAAAAGAAATATGGAAATCGGGGGGTAAGGGATGCAAATACCTCGCATGTCGCTTCGCCAGCTAGCCTGGTcc                                            >  1:2223515/1‑100 (MQ=255)
                                                     tGGCAGGTTGTCGGGTCGTGTTATGGAAACAGTAAGATGTATATCGGGGGGTACGGGTTGCAAATAAATCGCATGGCGCTTCGCCAGCTAGCCTgg                                               <  2:1429199/96‑1 (MQ=255)
                                                     tGGCAGGTTCTCTGGTCGTGTTATGGAAAAAGAAATATGGAAATCGGGGGTAAGGGATGCAAATACCTCGCATGTCGCTTCGCCAGCTAGCCTGGTCCgg                                          <  1:524274/100‑1 (MQ=255)
                                                      gggaGGTTCTCTGGTCGTGTTATGGAAAAAGAAATATGGAAATCGGGGGTAAGGGATGCAAATACCTCGCATGTCGCTTCGCCAGCTAGCCTGGTcc                                            <  2:239130/94‑1 (MQ=255)
                                                      ggCAGGTTCTCTGGTCGTGTTATGGAAAAAGAAATATGGAAATCGGGGGTAAGGGATGCAAATACCTCGCATGTCGCTTCGCCAGCTAGCCTGGTCCGgc                                         >  1:1009593/1‑100 (MQ=255)
                                                       gCAGGTTCTCTGGTCGTGTTATGGAAAAAGAAATATGGAAATCGGGGGGTAAGGGATGCAAATACCTCGCATGTCGCTTCGCCAGCTAGCCTGGTCCggn                                         <  2:1006150/100‑2 (MQ=255)
                                                           gTTCTCTGGTCGTGTTATGGAAAAAGAAATATGGAAATCGGGGGTAAGGGATGCAAATACCTCGCATGTCGCTTCGCCAGCTAGCCTGGTCCGGCGCTGt                                    >  1:3063532/1‑100 (MQ=255)
                                                              ctctGGTCGTGTTATGGAAAAAGAAATATGGAAATCGGGGGGTAGGGGATGCAAATACCTAGCATGTCGCTTCGCAAGCTAGCCTGGTCCGGCGCTGttt                                  >  2:746873/1‑100 (MQ=255)
                                                              ctctGGTCGTGTTATGGAAAAAGAAATATGGAAATCGGGGGGTAAGGGATGCAAATACCTCGCATGTCGCTTCGCCAGCTAGCCTGGTCCGGCGCTGttt                                  >  1:1574131/1‑100 (MQ=255)
                                                               tctGGTCGTGTTATGGAAAAAGAAATATGGAAATCGGGGGGTAAGGGATGCAAATACCTCGCATGTCGCTTCGCCAGCTAGCCTGGTCCGGCGCTGtttt                                 >  1:817390/1‑100 (MQ=255)
                                                                 tGGTCGTGTTATGGAAAAAGAAATATGGAAATCGGGGGGTAAGGGATGCAAATACCTCGCATGTCGCTTCGCCAGCTAGCCTGGTCCGGCGCTGTTTTAc                               >  1:2555426/1‑100 (MQ=255)
                                                                  ggtggtGTGATGGAAAAAGAAATATGGAAATCGGGGGTAAGGGATGCAAATACCTCGCATGTCGCTTCGCCAGCTAGCCTGGTCCGGCGCTGTTTTAc                               <  2:2542426/98‑1 (MQ=255)
                                                                  ggTCGTGTTATGGAAAAAGAAATATGGAAATCGGGGGGTAAGGGATGCAAATACCTCGCATGTCGCTTCGCCAGCTAGCCTGGTCCGGCGCTGTTTTACt                              >  1:2818210/1‑100 (MQ=255)
                                                                  ggTCGTGTTATGGAAAAAGAAATATGGAAATCGGGGGGTAAGGGATGCAAATACCTCGCATGTCGCTTCGCCAGCTAGCCTGGTCCGGCGCTGTTTTACt                              >  2:1238480/1‑100 (MQ=255)
                                                                  ggTCGTGTTATGGAAAAAGAAATATGGAAATCGGGGGTAAGGGATGCAAATACCTCGCATGTCGCTTCGCCAGCTAGCCTGGTCCGGCGCTGTTTTACtt                             >  1:2070919/1‑100 (MQ=255)
                                                                    tCGTGTTATGGAAAAAGAAATATGGAAATCGGGGGGTAAGGGATGCAAATACCTCGCATGTCGCTTCGCCAGCTAGCCTGGTCCGGCGCTGTTTTACTTc                            >  2:1820819/1‑100 (MQ=255)
                                                                     cGTGTTATGGAAAAAGAAATATGGAAATCGGGGGGTAAGGGATGCAAATACCTCGCATGTCGCTTCGCCAGCTAGCCTGGTCCGGCGCTGTTTTACTTCt                           <  1:1460067/100‑1 (MQ=255)
                                                                       tgtTATGGAAAAAGAAATATGGAAATCGGGGGGTAAGGGATGCAAATACCTCGCATGTCGCTTCGCCAGCTAGCCTGGTCCGGCGCTGTTTTACTTCTgg                         <  1:2447302/100‑1 (MQ=255)
                                                                        gtTAAGGAAAAAGAAATATGGAAATCGGGGGGTAAGGGATGCAAATACCTCGCATGTCGCTTCGCCAGCTAGCCTGGTCCGGCGCTGTTTTACTTCTgg                         >  2:2668473/1‑99 (MQ=255)
                                                                         ttATGGAAAAAGAAATATGGAAATCGGGGGTAAGGGATGCAAATACCTCGCATGTCGCTTCGCCAGGTAGCCTGGTCCGGCGCTGTTTTACTTCTGGTCg                      >  2:409556/1‑100 (MQ=255)
                                                                          tATGGAAAAAGAAATATGGAAATCGGGGGTAAGGGATGCAAATACCTCGCATGTCGCTTCGCCAGCTAGCCTGGTCCGGCGCTGTTTTACTTCTGGTCgg                     <  1:1386250/100‑1 (MQ=255)
                                                                            tGGAAAAAGAAATATGGAAATCGGGGGATAAGGGATGCAAATACCTCGCATGTCGCTTCGCCAGCTAGCCTGGTCCGGCGCTGTTTTACTTCTGGTCGGc                    >  1:682998/1‑100 (MQ=255)
                                                                             ggAAAAAGAAATATGGAAATCGGGGGGTAAGGGATGCAAATACCTCGCATGTCGCTTCGCCAGCTAGCCTGGTCCGGCGCTGTTTTACTTCTGGTCGGCa                   >  1:1531390/1‑100 (MQ=255)
                                                                              gAAAAAGAAATATGGAAATCGGGGGGTAAGGGATGCAAATACCTCGCATGTCGCTTCGCCAGCTAGCCTGGTCCGGCGCTGTTTTACTTCTGGTCGGCAc                  >  1:276037/1‑100 (MQ=255)
                                                                                aaaaGAAATATGGAAATCGGGGGGTAAGGGATGCAAATACCTCGCATGTCGCTTCGCCAGCTAGCCTGGTCCGGCGCTGTTTTACTTCTGGTCGGCACGc                >  2:1187470/1‑100 (MQ=255)
                                                                                aaaaGAAATATGGAAATCGGGGGGTAAGGGATGCAAATAACTCGCATGTCGCTTCGCCAGCTAGCCTGGTCAGGCGCTGTTTTACTTCTGGTCGGc                    >  2:692616/1‑96 (MQ=255)
                                                                                aaaaGAAATATGGAAATCGGGGGTAAGGGATGCAAATACCTCGCATGTCGCTTCGCCAGCTAGCCTGGTCCGGCGCTGTTTTACTTCTGGTCGGCACGCt               >  1:1569124/1‑100 (MQ=255)
                                                                                  aaGAAATATGGAAATCGGGGGTAAGGGATGCAAATACCTCGCATGTCGCTTCGCCAGCTAGCCTGGTCCGGCGCTGTTTTACTTCTGGTCGGCACGCTGt             <  2:2415290/100‑1 (MQ=255)
                                                                                    gAAATATGGAAATCGGGGGGTAAGGGATGCAAATACCTCGCATGTCGCTTCGCCAGCTAGCCTGGTCCGGCGCTGTTTTACTTCTGGTCGGCACGCTGtt            >  2:317063/1‑100 (MQ=255)
                                                                                      aaTATGGAAATCGGGGGGTAAGGGATGCAAATACCTCGCATGTCGCTTCGCCAGCTAGCCTGGTCCGGCGCTGTTTTACTTCTGGTCGGCACGCTGTTGc          >  1:2550932/1‑100 (MQ=255)
                                                                                      aaTATGGAAATCGGGGGGTAAGGGATGCAAATACCTCGCATGTCGCTTCGCCAGCTAGCCTGGTCCGGCGCTGTTTTACTTCTGGTCGGCACGCTGTTGc          <  1:1092824/100‑1 (MQ=255)
                                                                                       atatGGAAATCGGGGGGTAAGGGATGCAAATACCTCGCATGTCGCTTCGCCAGCTAGCCTGGTCCGGCGCTGTTTTACTTCTGGTCGGCACGCTGTTGCt         >  1:1079209/1‑100 (MQ=255)
                                                                                       atatGGAAATCGGGGGGTAAGGGATGCAAATACCTCGCATGTCGCTTCGCCAGCTAGCCTGGTCCGGCGCTGTTTTACTTCTGGTCGGCACGCTGTTGCt         >  1:2340561/1‑100 (MQ=255)
                                                                                       atatGGAAATCGGGGGTAAGGGATGCAAATACCTCGCATGTCGCTTCGCCAGCTAGCCTGGTCCGGCGCTGTTTTACTTCTGGTCGGCACGCTGTTGCTg        >  1:1188785/1‑100 (MQ=255)
                                                                                        tatGGAAATCGGGGGGTAAGGGATGCAAATACCTCGCATGTCGCTTCGCCAGCTAGCCTGGTCCGGCGCTGTTTTACTTCTGGTCGGCACGCTGTTGCTg        <  1:937499/100‑1 (MQ=255)
                                                                                         atGGAAATCGGGGGGTAAGGGATGCAAATACCTCGCATGTCGCTTCGCCAGCTAGCCTGGTCCGGCGCTGTTTTACTTCTGGTCGGCCCGCTGTTGCTgg       >  2:1788916/1‑100 (MQ=255)
                                                                                          tGGAAATCGGGGGGTAAGGGATGCAAATATCTCGCCTGTCGCTTCGCCAGCTAGCCTGGTGCGGCGGTGGTTTATTTCTGGTCGGCACGCTGTTGCt         >  2:529707/1‑97 (MQ=255)
                                                                                          tGGAAATCGGGGGGTAAGGGATGCAAATACCTCGCATGTCGCTTCGCCAGCTAGCCTGGTCCGGCGCTGTTTTACTTCTGGTCGGCACGCTGTTGCTGGc      >  1:1940677/1‑100 (MQ=255)
                                                                                          tGGAAATCGGGGGGTAAGGGATGCAAATACCTCGCATGTCGCTTCGCCAGCTAGCCTGGTCCGGCGCTGTTTTACTTCTGGTCGGCACGCTGTTGCTGGc      <  2:521386/100‑1 (MQ=255)
                                                                                          tGGAAATCGGGGGTAAGGGATGCAAATACCTCGCATGTCGCTTCGCCAGCTAGCCTGGTCCGGCGCTGTTTTACTTCTGGTCGGCACGCTGTTGCTGGcc     >  1:321248/1‑100 (MQ=255)
                                                                                           ggAAATCGGGGGGTAAGGGATGCAAATACCTCGCATGTCGCTTCGCCAGCTAGCCTGGTCCGGCGCTGTTTTACTTCTGGTCGGCACGCTGTTGCTGGcc     <  1:317065/100‑1 (MQ=255)
                                                                                           ggAAATCGGGGGGTAAGGGATGCAAATACCTCGCATGTCGCGTCGCCAGCTAGCCTGGTCCGGCGCTGTTTTACTTCTGGTCGGCACGCTGTTGCTGGcc     <  1:609420/100‑1 (MQ=255)
                                                                                            gAAATCGGGGGGTAAGGGATGCAAATACCTCGCATGTCGCTTCGCCAGCTAGCCTGGTCCGGCGCTGTTTTACTTCTGGTCGGCACGCTGTTGCTGGCCt    >  1:1075046/1‑100 (MQ=255)
                                                                                             aaaTCGGGGGGTAAGGGATGCAAATACCTCGCATGTCGCTTCGCCAGCTAGCCTGGTCCGGCGCTGTTTTACTTCTGGTCGGCACGCGGTTGCTGGc      >  2:39408/1‑97 (MQ=255)
                                                                                             aaaTCGGGGGTAAGGGATGCACATACCTCGCATGTCGCTTCGCCTGCTAGCCTGGTCCGGCGCTGTTTTACTTCTGGTCGGCACGCTGTTGCTGGCCTgg  <  1:2481782/100‑1 (MQ=255)
                                                                                             aaaTCGGGGGTAAGGGATGCAAATACCTCGCATGTCGCTTCGCCAGCTAGCCTGGTCCGGCGCTGTTTTACTTCTGGTCGGCACGCTGTTGCTGGCCTg   >  1:30659/1‑99 (MQ=255)
                                                                                                  |                                                                                                 
CCAGCTGATGTCATGCCTGATGCTGCTCCCGGTGGTGCTGCTGGTGTTTGGCCTGGCAGGTTCTCTGGTCGTGTTATGGAAAAAGAAATATGGAAATCGGGGGTAAGGGATGCAAATACCTCGCATGTCGCTTCGCCAGCTAGCCTGGTCCGGCGCTGTTTTACTTCTGGTCGGCACGCTGTTGCTGGCCTGG  >  NC_000913/3248330‑3248522

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: