BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	1,756,553	A→T	100%	K286* (AAA→TAA)	pykF →	pyruvate kinase I

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,756,553	0	A	T	99.4%	366.0 / ‑6.1	107	K286* (AAA→TAA)	pykF	pyruvate kinase I
Reads supporting (aligned to +/- strand): ref base A (1/0); new base T (58/48); total (59/48)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01

Only 100 of 107 total aligned reads displayed.

AGAAGTTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTGAATCCGCAAAAG  >  NC_000913/1756459‑1756649

agaagTTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCTAAAAc                                                                                             >  1:2820843/1‑100 (MQ=255)

   agTTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCTAAAACCCa                                                                                          <  2:309139/100‑1 (MQ=255)

     ttATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCTAAAACCCACg                                                                                        <  1:1625650/100‑1 (MQ=255)

      tATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCTAAAACCCACGc                                                                                       >  1:2842179/1‑100 (MQ=255)

      tATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCTAAAACCCACGc                                                                                       >  1:2461301/1‑100 (MQ=255)

      tATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCTAAAACCCACGc                                                                                       <  1:1458848/100‑1 (MQ=255)

       aTCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCTAAAACCCACGcc                                                                                      <  2:1791553/100‑1 (MQ=255)

       aTCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCTAAAACCCACGcc                                                                                      <  1:2708375/100‑1 (MQ=255)

        tCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCTAAAACCCACGccc                                                                                     >  1:960817/1‑100 (MQ=255)

        tCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCTAAAACCCACGccc                                                                                     >  2:1721128/1‑100 (MQ=255)

         cTTCGCCCAGAAGATGATTATCGAAAAATGCAGCCGGGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCTATAACCCACGccc                                                                                     <  2:1398890/99‑1 (MQ=255)

         cTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCTAAAACCCACGCCCg                                                                                    >  1:2550922/1‑100 (MQ=255)

           tctcccAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCTAAAACCCACGCCCGAc                                                                                  <  1:2638424/97‑1 (MQ=255)

            cGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCTAAAACCCACGCCCGAc                                                                                  <  2:833888/99‑1 (MQ=255)

             gCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCTAAAACCCACGCCCGACTc                                                                                >  1:1327401/1‑100 (MQ=255)

              cccAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCTAAAACCCACGCCCGAcaga                                                                               >  2:2638396/1‑97 (MQ=255)

               ccAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCTAAAACCCACGCCCGACTcgc                                                                              <  1:2488763/100‑1 (MQ=255)

                cAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCTAAAACCCACGCCCGACTcgcg                                                                             <  2:1277051/100‑1 (MQ=255)

                cAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCTAAAACCCACGCCCGACTcgcg                                                                             <  2:400364/100‑1 (MQ=255)

                 agaagaTGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCTAAAACCCACGCCCGACTCgcgc                                                                            <  1:1920987/100‑1 (MQ=255)

                   aagaTGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCTAAAACCCACGCCCGACTCGCGCAg                                                                          <  1:1740852/100‑1 (MQ=255)

                   aagaTGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCTAAAACCCACGCCCGACTCGCGCAg                                                                          <  1:1325165/100‑1 (MQ=255)

                    agaTGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCTAAAACCCACGCCCGACTCGCGCAGa                                                                         <  2:2149704/100‑1 (MQ=255)

                    agaTGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCTAAAACCCACGCCCGACTCGCGCAGa                                                                         <  2:2705704/100‑1 (MQ=255)

                     gatgatgatCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCTAAAACCCACGCCCGACTCGCGCAGaa                                                                        <  2:2334779/100‑1 (MQ=255)

                       tgatgatCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCTCAAACCCACGCCCGACTCGCGCAGaa                                                                        >  2:616876/1‑98 (MQ=255)

                       tgatgatCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCTAAAACCCACGCCCGACTCGCGCAGAAGc                                                                      >  2:1857638/1‑100 (MQ=255)

                       tgatgatCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCTAAAACCCACGCCCGACTCGCGCAGAAGc                                                                      >  1:989584/1‑100 (MQ=255)

                         atgatCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCTAAAACCCACGCCCGACTCGCGCAGAAGCCg                                                                    >  1:2078631/1‑100 (MQ=255)

                          tgatCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCTAAAACCCACGCCCGACTCGCGCAGAAGCCgg                                                                   >  1:1480063/1‑100 (MQ=255)

                          tgatCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCTAAAACCCACGCCCGACTCGCGCAGAAGCCgg                                                                   <  1:915233/100‑1 (MQ=255)

                          tgatCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCTAAAACCCACGCCCGACTCGCGCAGAAGCCgg                                                                   >  1:1242475/1‑100 (MQ=255)

                           gatCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCTAAAACCCACGCCCGACTCGCGCAGAAGCCGGt                                                                  >  1:1310857/1‑100 (MQ=255)

                            atCTAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCTAAAACCCACGCCCGACTCGCGCAGAAGCCGGTg                                                                 <  1:939224/100‑1 (MQ=255)

                            atCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCTAAAACCCACGCCCGACTCGCGCAGAAGCCGGTg                                                                 >  2:2033470/1‑100 (MQ=255)

                            atCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCTAAAACCCACGCCCGACTCGCGCAGAAGCCGGTg                                                                 >  1:3075540/1‑100 (MQ=255)

                            atCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCTAAAACCCACGCCCGACTCGCGCAGAAGCCGGTg                                                                 <  2:356527/100‑1 (MQ=255)

                              cGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCTAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGAc                                                               <  2:1300517/100‑1 (MQ=255)

                               gAAAAATGTATCCGTGCACGTACAGTCGTTATCACTGCGACCCACATACTGGATTCCATGATCTAAAACCCACCCCCGACTCGCGCAGAAGCCGGTg                                                                 >  2:2037911/1‑97 (MQ=255)

                               gAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCTAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACg                                                              >  2:1337855/1‑100 (MQ=255)

                                 naaaTGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCTAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGtt                                                            >  2:807077/2‑100 (MQ=255)

                                  aaaTGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCTAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTg                                                           <  1:1353893/100‑1 (MQ=255)

                                  aaaTGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCTAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTg                                                           <  2:2819677/100‑1 (MQ=255)

                                  aaaTGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCTAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTg                                                           <  1:2432619/100‑1 (MQ=255)

                                  aaaTGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCTAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTg                                                           <  1:1723800/100‑1 (MQ=255)

                                    aTGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCTAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCa                                                         >  1:591396/1‑100 (MQ=255)

                                        aTCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCTAAAACCCACGCCCGACTCGGGCAGAAGCCGGTGACGTTGCAAACg                                                     >  2:1585991/1‑100 (MQ=255)

                                         tCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCTAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGc                                                    >  1:3102789/1‑100 (MQ=255)

                                          ccGTGCACGTAAAGTCGTTATCACTGCGACCCACATGCTGGATTCCATGATCTAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACg                                                     >  2:2833488/1‑98 (MQ=255)

                                            gTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCTAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCAt                                                 >  2:1026427/1‑100 (MQ=255)

                                              gCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCTAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCAt                                                 >  1:1855075/1‑98 (MQ=255)

                                              gCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCTAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATcc                                               <  2:2147476/100‑1 (MQ=255)

                                               cACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCTAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCt                                              >  2:2175668/1‑100 (MQ=255)

                                                aCGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCTAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTc                                             <  1:1563858/100‑1 (MQ=255)

                                                aCGTAAAGTCGTTATCACTGCGACCCAGATGCGGGATTCAATTATCTAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTc                                             <  2:2184295/100‑1 (MQ=255)

                                                 cGTACAGTCGTTATAACTGCGACCCAGATGCTGGATTCCATGATCTAAAACCCGCGCCCGACTCGCGGAGAAGCCTGTGACGTTGCAAACGCCATACTCg                                            <  2:2836804/100‑1 (MQ=255)

                                                  gTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCTAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGa                                           >  2:747291/1‑100 (MQ=255)

                                                   tAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCTAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGAc                                          <  2:450788/100‑1 (MQ=255)

                                                    aaaGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCTAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACg                                         >  2:391384/1‑100 (MQ=255)

                                                      aGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCTAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGt                                       >  1:1112404/1‑100 (MQ=255)

                                                        tCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCTAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTAc                                     <  1:2562843/100‑1 (MQ=255)

                                                          gTTATCACTGCGACCCAGATGCTGGATTCCATGATCTAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTg                                   >  2:2426935/1‑100 (MQ=255)

                                                          gTTATCACTGCGACCCAGATGCTGGATTCCATGATCTAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTg                                   >  2:2574811/1‑100 (MQ=255)

                                                          gTTATCACTGCGACCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGGCGTTGCAAACGCCATCCTCGACGGTAc                                     >  2:1874097/1‑98 (MQ=255)

                                                            tATCACTGCGACCCAGATGCTGGATTCCATGATCTAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGAc                                 >  2:1741179/1‑100 (MQ=255)

                                                            tATCACTGCGACCCAGATGCTGGATTCCATGATCTAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGAc                                 >  2:2286765/1‑100 (MQ=255)

                                                              tCACTGCGACCCAGATGCTGGATTCCATGATCTAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGACg                                >  2:1884241/1‑99 (MQ=255)

                                                              tCACTGCGACCCAGATGCTGGATTCCATGATCTAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGACGc                               <  1:2603103/100‑1 (MQ=255)

                                                               cACTGCGACCCAGATGCTGGATTCCATGATCTAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGACGCa                              >  2:2884610/1‑100 (MQ=255)

                                                                aCTGCGACCCAGATGCTGGATTCCATGATCTAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGACGCAg                             <  2:2306854/100‑1 (MQ=255)

                                                                aCTGCGACCCAGATGCTGGATTCCATGATCTAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGACGCAg                             >  1:1198116/1‑100 (MQ=255)

                                                                aCTGCGACCCAGATGCTGGATTCCATGATCTAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGACGCAg                             <  1:239015/100‑1 (MQ=255)

                                                                 cTGCGACCCAGATGCTGGATTCCATGATCTAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGACGCAGt                            <  2:1980040/100‑1 (MQ=255)

                                                                  tGCGACCCAGATGCTGGATTCCATGATCTAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGACGCAGTg                           <  2:2426543/100‑1 (MQ=255)

                                                                     gACCCAGATGCTGGATTCCATGATCTAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATg                        >  1:798112/1‑100 (MQ=255)

                                                                      aCCCAGATGCTGGATTCCATGATCTAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAATGCCATCCTCGACGGTACTGACGCAGTGAttc                       <  1:2094778/100‑3 (MQ=255)

                                                                       cccAGATGCTGGATTCCATGATCTAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCt                      >  1:2509289/1‑100 (MQ=255)

                                                                        ccAGATGCTGGATTCCATGATCTAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCt                      >  2:944615/1‑99 (MQ=255)

                                                                        ccAGATGCTGGATTCCATGATCTAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTg                     <  2:2944948/100‑1 (MQ=255)

                                                                        ccAGATGCTGGATTCCATGATCTAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTg                     <  1:905930/100‑1 (MQ=255)

                                                                          aGATGCTGGATTCCATGATCTAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTc                   >  1:847743/1‑100 (MQ=255)

                                                                             tGCTGGATTCCATGATCTAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTgg                <  1:573624/100‑1 (MQ=255)

                                                                             tGCTGGATTCCATGATCTAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTgg                >  2:823352/1‑100 (MQ=255)

                                                                               cTGGATTCCATGATCTAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTg              >  2:2834200/1‑100 (MQ=255)

                                                                               cTGGATTCCATGATCTAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTg              <  2:2382272/100‑1 (MQ=255)

                                                                               cTGGATTCCATGATCTAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTg              >  1:1601994/1‑100 (MQ=255)

                                                                               cTGGATGCCATGCTCTAAAACCCACGCCCGACTCGCACAGAAGCCTGTGACATTCCAAACGCCATCCTCGACGGGACTGACGCAGAGATGCTGTCTgg                >  2:2834712/1‑98 (MQ=255)

                                                                                 ggATTCCATGATCTAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTGaa            >  1:1479010/1‑100 (MQ=255)

                                                                                   aTTCCATGATCTAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTGAATc          >  2:2125431/1‑100 (MQ=255)

                                                                                   aTTCCATGATCTAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGAAGTTGCAAACGCCATCCTCGACGGTACTGACGCAATGATGCTGACTGGTGAATc          >  2:2564655/1‑100 (MQ=255)

                                                                                    ttCCATGATCTAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTGAATcc         <  1:2803707/100‑1 (MQ=255)

                                                                                      ccATGATCTAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTGAATCCGc       >  1:97657/1‑100 (MQ=255)

                                                                                      ccATGATCTAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTGAATCCGc       >  1:41526/1‑100 (MQ=255)

                                                                                      ccATGATCTAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTGAATCCGc       >  1:223262/1‑100 (MQ=255)

                                                                                       cATGATCTAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTGAATCCGCa      <  2:212050/100‑1 (MQ=255)

                                                                                       cATGATCTAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTGAATCCGCa      <  2:1622123/100‑1 (MQ=255)

                                                                                       cATGATCTAAAACCCACGCCCGACTCGCGCAGAAGCCGATGACTTCGCAAACGCCATCCCCGACTGTACTGACGCAGTGTTGCTGGCTGGTGATTCCGc       >  2:1365337/1‑99 (MQ=255)

                                                                                         tGATCTAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTGAATCCGCaaa    >  2:7950/1‑100 (MQ=255)

                                                                                           aTCTAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTGAATCCGCAAAAg  >  1:2149815/1‑100 (MQ=255)

                                                                                           aTCTAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTGAATCCGCAAAAg  >  2:313298/1‑100 (MQ=255)

AGAAGTTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTGAATCCGCAAAAG  >  NC_000913/1756459‑1756649

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`