Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,057,603 G→A 15.9% intergenic (+29/+424) yeeN → / ← asnW UPF0082 family protein/tRNA‑Asn

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,057,6030GA15.9% 349.0 / 46.2 149intergenic (+29/+424)yeeN/asnWUPF0082 family protein/tRNA‑Asn
Reads supporting (aligned to +/- strand):  ref base G (77/47);  new base A (6/18);  total (84/65)
Fisher's exact test for biased strand distribution p-value = 1.29e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.69e-01

Only 100 of 153 total aligned reads displayed.

AAGGGCTTGTTGATGCCCTTGAAGATGACGACGATGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATACCCCCCCGTTCCCAGAGAAATAATATTTATTAA  >  NC_000913/2057504‑2057702
                                                                                                   |                                                                                                   
  gggCTTGTTGATGCCCTTGAAGATGACGACGATGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCa                                                                                                   >  1:1924176/1‑100 (MQ=255)
    gCTTGTTGATGCCCTTGAAGATGACGACGATGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGa                                                                                                 >  1:2802224/1‑100 (MQ=255)
     cTTGTTGATGCCCTTGAAGATGACGACAATGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGAt                                                                                                <  2:238855/100‑1 (MQ=255)
       tgttgATGCCCTTGAAGATGACGACGATGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGAttt                                                                                              >  2:2937275/1‑100 (MQ=255)
           gATGCCCTTGAAGATGACGACGATGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCttt                                                                                          >  2:35211/1‑100 (MQ=255)
           gATGCCCTTGAAGATGACGACGATGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCttt                                                                                          >  1:3008701/1‑100 (MQ=255)
           gATGCCCTTGAAGATGACGACGATGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGCCGTAAAGACAGATTTCttt                                                                                          <  2:959445/100‑1 (MQ=37)
           gATGCCCTTGAAGATGACGACGATGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGCCGTAAAGACAGATTTCttt                                                                                          <  2:2475398/100‑1 (MQ=37)
           gATGCCCTTGAAGATGACGACGATGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGCCGTAAAGACAGATTTCttt                                                                                          <  1:1839294/100‑1 (MQ=37)
           gATGCCCCTGAAGATGAAGACGATGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGCCGTAAAGACAGATTTCttt                                                                                          <  2:189582/100‑1 (MQ=37)
            aTGCCCTTGAAGATGACGACGATGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTa                                                                                         >  1:3074766/1‑100 (MQ=255)
            aTGCCCTTGAAGATGACGACGATGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGCCGTAAAGACAGATTTCTTTa                                                                                         <  1:2075805/100‑1 (MQ=37)
             tGCCCTTGAAGATGACGACGATGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGAtcggaagag                                                                                        >  2:775217/1‑92 (MQ=255)
              gCCCTTGAAGATGACGACGATGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGCCGTAAAGACAGATTTCTTTaaa                                                                                       <  1:112508/100‑2 (MQ=37)
               cccTTGAAGATGACGACGATGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAAtc                                                                                      >  2:2063617/1‑100 (MQ=255)
               cccTTGAAGATGACGACGATGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGCCGTAAAGACAGATTTCTTTaaaa                                                                                      <  1:757535/100‑3 (MQ=25)
               cccTTGAAGATGACGACGATGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGCCGTAAAGACAGATTTCTTTaaaa                                                                                      <  1:1426531/100‑3 (MQ=25)
                 cTTGAAGATGGCGACGATGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAAtctc                                                                                    <  2:1420686/100‑1 (MQ=255)
                  ttGAAGATGACGACGATGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCa                                                                                   <  2:587615/100‑1 (MQ=255)
                  ttGAAGATGACGACGATGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCa                                                                                   <  1:2224950/100‑1 (MQ=255)
                    gAAGATGACGACGATGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAGGAAATGTGTATTTACGGCAGATTTCTTTAATCTCata                                                                                 >  1:2770275/1‑100 (MQ=255)
                     aaGATGACGACGATGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCatat                                                                                >  1:1056287/1‑100 (MQ=255)
                     aaGATGACGACGATGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCatat                                                                                >  2:2509712/1‑100 (MQ=255)
                          gacgacGATGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTc                                                                           <  2:1429246/100‑1 (MQ=255)
                          gacgacGATGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTc                                                                           <  2:1512295/100‑1 (MQ=255)
                          gacgacGATGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTc                                                                           >  1:2706285/1‑100 (MQ=255)
                            cgacgaTGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCtt                                                                         <  1:264933/100‑1 (MQ=255)
                            cgacgaTGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCtt                                                                         >  1:683745/1‑100 (MQ=255)
                            cgacgaTGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCtt                                                                         >  1:1761121/1‑100 (MQ=255)
                             gacgaTGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTta                                                                        <  1:1216278/100‑1 (MQ=255)
                              acgaTGTACAAAAATTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTta                                                                        <  2:1479812/99‑1 (MQ=255)
                               gatcttACAAAAAGTTTATCTTAACGTAGCAAATCTCTAATTATCTTTTAAAGAAATATGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTtata                                                                      <  2:256778/95‑1 (MQ=255)
                               cgaTGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTtata                                                                      >  1:2616808/1‑100 (MQ=255)
                                 aTGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATaaa                                                                    >  1:1042176/1‑100 (MQ=255)
                                  tGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATaaaa                                                                   <  1:1440569/100‑1 (MQ=255)
                                    tACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATaagatc                                                                 >  1:256780/1‑96 (MQ=255)
                                    tACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATaaaaaa                                                                 <  1:1870184/100‑1 (MQ=255)
                                      cAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTTTTATaaaaaa                                                                 >  1:2530775/1‑98 (MQ=255)
                                      cAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAaata                                                               >  1:787622/1‑100 (MQ=255)
                                      cAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAaat                                                                >  2:1775147/1‑99 (MQ=255)
                                       aaaaaGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAaatat                                                              <  2:1160154/100‑1 (MQ=255)
                                        aaaaGTTTATCATAACGTCGCAAATCGCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAaatata                                                             <  2:1572970/100‑1 (MQ=255)
                                        aaaaGTTTACCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCCGATTTCTTTAATCTCATATAATTCTTATAAAAaatata                                                             >  2:827911/1‑100 (MQ=255)
                                         aaaGTTTATCATCACGTGGCAAATCTCAAAATATCTTTTAAAGAAATCTGTCTTTACAACAGATTTCTTTAATCTCATATAATTCTTATAAAAaatataa                                                            <  2:1780927/100‑1 (MQ=255)
                                         aaaGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAaatataa                                                            <  1:2631596/100‑1 (MQ=255)
                                         aaaGTTTAACATAACGTCTCTAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACAGCAGATTTCTTTAATCTCATATAATTCTTATAAAAaatata                                                             <  2:346843/99‑1 (MQ=255)
                                           aGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAaatataata                                                          >  2:1583140/1‑100 (MQ=255)
                                           aGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAaatataat                                                           <  2:2856329/99‑1 (MQ=255)
                                            gTTTATCATAACGTCGCAACTCTCTAATTATCTTTTAAAGAAATCTATCTCTACGGCAGATTTATTTAATCTCATATAATTCTTATAAAAaatatactc                                                          >  2:1142940/1‑96 (MQ=255)
                                            gTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAaatataatat                                                         >  2:1206360/1‑100 (MQ=255)
                                            gTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAaatataatat                                                         <  1:2522030/100‑1 (MQ=255)
                                              ttATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTc                                                       <  1:668380/100‑1 (MQ=255)
                                                aTCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCaa                                                     <  2:2610987/100‑1 (MQ=255)
                                                  cATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACt                                                   >  1:570586/1‑100 (MQ=255)
                                                   aTAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTc                                                  >  1:3002946/1‑100 (MQ=255)
                                                    tAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATTTCATTTTATTCTTATATCAAATAAGATATTCAACTc                                                  >  2:1967962/1‑99 (MQ=255)
                                                    tAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCg                                                 >  1:1298198/1‑100 (MQ=255)
                                                    tAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCg                                                 >  1:943033/1‑100 (MQ=255)
                                                    tAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCg                                                 >  2:841975/1‑100 (MQ=255)
                                                      aCGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTc                                               <  1:2221498/100‑1 (MQ=255)
                                                        gTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCat                                             <  2:104219/100‑1 (MQ=255)
                                                        gTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCat                                             <  1:514915/100‑1 (MQ=255)
                                                         tctcAAATCTTTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCat                                             <  2:702511/96‑1 (MQ=255)
                                                          cGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCatat                                           >  1:1567957/1‑100 (MQ=255)
                                                          cGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCatat                                           >  2:2832364/1‑100 (MQ=255)
                                                          cGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCatat                                           >  2:922310/1‑100 (MQ=255)
                                                          cGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCatat                                           >  1:1324893/1‑100 (MQ=255)
                                                           gCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATAtt                                          >  2:2496862/1‑100 (MQ=255)
                                                             taaTCTCTAATTATCTTTTAAAGAAATCTGTCTTTACTGGAGATTTCTTTAATCTCACATAATTCTTATAAAAAATATAATATTCAACTCGTCATAtt                                          <  2:454334/97‑1 (MQ=255)
                                                               aTCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATAATAATAAAAAATATAATATTCAACTCGTCATATTGAtt                                      <  2:1736724/100‑1 (MQ=255)
                                                                tctcTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATta                                     <  1:622693/100‑1 (MQ=255)
                                                                  tctAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATATAATATAATTCTTATAAAACATATATTATTCAACTTGTCATATTGATtata                                   >  2:715000/1‑100 (MQ=255)
                                                                   ctAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATAc                                  >  1:1194388/1‑100 (MQ=255)
                                                                   ctAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATAc                                  >  1:1880720/1‑100 (MQ=255)
                                                                       tatgagattAAAGAAATCTGCCGTAAAGACAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCCTCATATTGATTATaccacc                              >  2:2173817/8‑100 (MQ=18)
                                                                        tATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATAcccccc                             >  1:2097532/1‑100 (MQ=255)
                                                                        atgagattAAAGAAATCTGCCGTAAAGACAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACCCGTCATATTAATGATAtcccc                              >  2:1358134/7‑99 (MQ=18)
                                                                         tgagattAAAGAAATCTGCCGTAAAGACAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATAccccccc                            >  1:244020/6‑100 (MQ=21)
                                                                          tCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATACCCCCCCg                           <  2:1195186/100‑1 (MQ=255)
                                                                            ttttAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATACCCCCCCGtt                         >  1:3094997/1‑100 (MQ=255)
                                                                            ttttAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATACCCCCCCGtt                         >  1:947936/1‑100 (MQ=255)
                                                                              ttAAAGAAATCTGTCTTTACGACAAATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATACCCCCCCGTTca                       <  2:2770247/100‑2 (MQ=255)
                                                                               tAAAGAAATCTGCCGTAAAGACAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATACCCCCCCGTTccc                      >  1:390305/1‑100 (MQ=37)
                                                                                aaagcagtgtGTCTTTACGGCAGATGTCTTTAATCTCAGATCATTCCTATAAAAAATATAATATTCAACTCGTCATATTGATTATACCCCCCCGTTCCCa                     <  2:872439/91‑1 (MQ=255)
                                                                                 aaGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATACCCCCCCGTTCCCag                    <  2:1414357/100‑1 (MQ=255)
                                                                                 aaGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATACCCCCCCGTTCCCa                     >  2:2450579/1‑99 (MQ=255)
                                                                                 aaGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATACACCCCCGTTCCCag                    >  2:391397/1‑100 (MQ=255)
                                                                                  ataaaTCTGTCTTTACTGCAGATTTCTTTAATTTCATATAATTCTAATAAAAAATATAATATTCAACTCGTCATATTGATTATACCCCCCCGTTCCCag                    <  2:2001734/97‑1 (MQ=255)
                                                                                      aTCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATACCCCCCCGTTCCCAGAGaaa               >  1:2588517/1‑100 (MQ=255)
                                                                                             tttACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAACTATAATATTCAACTCGTCATATTGATTATACCCCCCCGTTCCCAGAGAAATAAtatt        <  2:3047478/100‑1 (MQ=255)
                                                                                             tttACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATACCCCCCCGTTCCCAGAGAAATAAtatt        >  1:172895/1‑100 (MQ=255)
                                                                                                aCGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATACCCCCCCGTTCCCAGAGAAATAAtatttat     <  1:1250281/100‑1 (MQ=255)
                                                                                                aCGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATACCCCCCCGTTCCCAGAGAAATAAtatttat     >  2:2338358/1‑100 (MQ=255)
                                                                                                aCGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATACCCCCCCGTTCCCAGAGAAATAAtatttat     >  1:1545206/1‑100 (MQ=255)
                                                                                                aCGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATACCCCCCCGTTCCCAGAGAAATAAtatttat     >  1:2614339/1‑100 (MQ=255)
                                                                                                 cGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATACCTCCCCGTTCCCAGAGAAATAAtatttatt    >  2:2882114/1‑100 (MQ=255)
                                                                                                 cGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATACCCCCCCGTTCCCAGAGAAATAAtatttatt    >  1:1554795/1‑100 (MQ=255)
                                                                                                 cGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATACCCCCCCGTTCCCAGAGAAATAAtatttatt    >  1:2501808/1‑100 (MQ=255)
                                                                                                  ggCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATACCCCCCCGTTCCCAGAGAAATAATATttatta   >  2:2711116/1‑100 (MQ=255)
                                                                                                   gCAGATTTCTTTAATCTCACATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATACCCCCCCGTTCCCAGAGAAATAATATTTATTaa  >  2:1740980/1‑100 (MQ=255)
                                                                                                   |                                                                                                   
AAGGGCTTGTTGATGCCCTTGAAGATGACGACGATGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATACCCCCCCGTTCCCAGAGAAATAATATTTATTAA  >  NC_000913/2057504‑2057702

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: