BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	2,754,639	C→A	100%	Q42H (CAG→CAT)	ratA ←	toxic UPF0083 family protein inhibitor of 70S ribosome formation

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,754,639	0	C	A	100.0%	399.7 / NA	114	Q42H (CAG→CAT)	ratA	toxic UPF0083 family protein inhibitor of 70S ribosome formation
Reads supporting (aligned to +/- strand): ref base C (0/0); new base A (58/56); total (58/56)

Only 100 of 114 total aligned reads displayed.

ACGTTTTGCTGATCCCAGCCTTAGAGACATCTACCGCCGCAGTCATCTGCCCAGGAGTGGACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAACTGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCTCCGCGCTGTAGGGTACCAGTGCGGTCCGGCTAATCTGAGGCATAACAAT  >  NC_000913/2754542‑2754731

aCGTTTTGCTGATCCCAGCCTTAGAGACATCTACCGCCGCAGTCATCTGCCCAGGAGTGGACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATg                                                                                            <  1:1934113/100‑1 (MQ=255)

  gTTTTGCTGATCCCAGCCTTAGCGACATCTACCGCCGCAGTCATCTGCCCAGGAGTGGACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAg                                                                                          <  1:2477971/100‑1 (MQ=255)

  gTTTTGCTGATCCCAGCCTTAGAGACATCTACCGCCGCAGTCATCTGCCCAGGAGTGGACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAg                                                                                          <  2:1043893/100‑1 (MQ=255)

  gTTTTGCTGATCCCAGCCTTAGAGACATCTACCGCCGCAGTCATCTGCCCAGGAGTGGACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAg                                                                                          >  1:2261595/1‑100 (MQ=255)

      tGCTGATCCCAGCCTTAGAGACATCTACCGCCGCAGTCATCTGCCCAGGAGTGGACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATa                                                                                      >  2:478246/1‑100 (MQ=255)

       gCTGATCCCAGCCTTAGAGACATCTACCGCCGCAGTCATCTGCCCAGGAGTGGACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATaa                                                                                     >  2:2790908/1‑100 (MQ=255)

       gCTGATCCCAGCCTTAGAGACATCTACCGCCGCAGTCATCTGCCCAGGAGTGGACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATaa                                                                                     >  2:3063486/1‑100 (MQ=255)

       gCTGATCCCAGCCTTAGAGACATCTACCGCCGCAGTCATCTGCCCAGGAGTGGACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATaa                                                                                     <  1:1822255/100‑1 (MQ=255)

        cTGATCCCAGCCTTAGAGACATCTACCGCCGCAGTCATCTGCCCAGGAGTGGACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAg                                                                                    <  1:520007/100‑1 (MQ=255)

         tGATCCCAGCCTTAGAGACATCTACCGCCGCAGTCATCTGCCCAGGAGTGGACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGa                                                                                   >  2:80781/1‑100 (MQ=255)

         tGATCCCAGCCTTAGAGACATCTACCGCCGCAGTCATCTGCCCAGGAGTGGACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGa                                                                                   >  2:483620/1‑100 (MQ=255)

         tGATCCCAGCCTTAGAGACATCTACCGCCGCAGTCATCTGCCCAGGAGTGGACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGa                                                                                   >  1:870084/1‑100 (MQ=255)

          gATCCCAGCCTTAGAGACATCTACCGCCGCAGTCATCTGCCCAGGAGTGGACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGAc                                                                                  <  1:1266947/100‑1 (MQ=255)

          gATCCCAGCCTTAGAGACATCTACCGCCGCAGTCATCTGCCCAGGAGTGGACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGAc                                                                                  >  2:675948/1‑100 (MQ=255)

          gATCCCAGCCTTAGAGACATCTACCGCCGCAGTCATCTGCCCAGGAGTGGACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGAc                                                                                  <  2:2940537/100‑1 (MQ=255)

             cccAGCCTTAGAGACATCTACCGCCGCAGTCATCTGCCCAGGAGTGGACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAACATGAGGATAAGACTGa                                                                               >  2:1744097/1‑100 (MQ=255)

             cccAGCCTTAGAGACATCTACCGCCGCAGTCATCTGCCCAGGAGTGGACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGa                                                                               >  2:741131/1‑100 (MQ=255)

              ccAGCCTTAGAGACATCTACCGCCGCAGTCATCTGCCCAGGAGTGGACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGaa                                                                              <  1:2517217/100‑1 (MQ=255)

                   cTTAGAGACATCTACCGCCGCAGTCATCTGCCCAGGAGTGGACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTc                                                                          >  1:1248614/1‑99 (MQ=255)

                    ttAGAGACATCTACCGCCGCAGTCATCTGCCCAGGAGTGGACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCAt                                                                        <  1:2928572/100‑1 (MQ=255)

                     tAGAGACATCTACCGCCGCAGTCATCTGCCCAGGAGTGGACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTc                                                                          <  2:272615/97‑1 (MQ=255)

                     tAGAGACATCTACCGCCGCAGTCATCTGCCCAGGAGTGGACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCAtt                                                                       >  2:1777857/1‑100 (MQ=255)

                     tAGAGACATCTACCGCCGCAGTCATCTGCCCAGGAGTGGACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCAtt                                                                       <  1:998954/100‑1 (MQ=255)

                      agagACATCTACCGCCGCAGTCATCTGCACAGGAGAGGACTCCCGAATCCGACTTCCGGTACAACCAGGCAAAAAATGAGCATAAGACTGAACGTc                                                                          >  2:509043/1‑96 (MQ=255)

                       gagaCATCTACCGCCGCAGTCATCTGCCCAGGAGTGGACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCa                                                                     >  1:847859/1‑100 (MQ=255)

                         gaCATCTACCGCCGCAGTCATCTGCCCAGGAGTGGACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACt                                                                   <  2:2363788/100‑1 (MQ=255)

                          aCATCTACCGCCGCAGTCATCTGCCCAGGAGTGGACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCAc                                                                    <  1:3019414/98‑1 (MQ=255)

                           cATCTACCGCCGCAGTCATCTGCCCAGGAGTGGACTCGAGAATCCGACCTCCGGTACAACCCGGCCAAAAATGAGGATAAGACTGAACGTCATTCACTaa                                                                 >  2:101561/1‑100 (MQ=255)

                           cATCTACCGCCGCAGTCATCTGCCCAGGAGTGGACTCCAGAATCCGACTTCCGGTACAACCAGGCAAAAAATGAGAATAAGACTGAACGTCCTTCAcca                                                                  >  2:2575209/1‑97 (MQ=255)

                            aacatccgcagAAGTCATCTGCCAAGGAGTGGACTCCAGAATCCGACTTCCGGTAAAAAACAACAAAAAATGAGGATAAGACTGAACGTCATTCACTAAc                                                                <  2:1521812/95‑1 (MQ=255)

                            aTCTACCGCCGCAGTCATCTGCCCAGGAGTGGACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAAc                                                                <  1:1572972/100‑1 (MQ=255)

                            aTCTACCGCCGCAGTCATCTGCCCAGGAGTGGACTCCAGAATCCGACTTCCAAAAAAAAAAAAAAAAAAATGAGGATAAGACTGAACGTCATTCACTAAc                                                                <  2:2155501/100‑1 (MQ=255)

                             tCTACCGCCGCAGTCATCTGCCCAGGAGTGGACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACt                                                               <  1:2708320/100‑1 (MQ=255)

                              cTACCGCCGCAGTCATCTGCCCAGGAGTGGACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTg                                                              <  1:1441436/100‑1 (MQ=255)

                              cTACCGCCGCAGTCATCTGCCCAGGAGTGGACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTg                                                              <  1:3045641/100‑1 (MQ=255)

                               tACCGCCGCAGTCATCTGCCCAGGAGTGGACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTg                                                              >  1:155338/1‑99 (MQ=255)

                               tACCGCCGCAGTCATCTGCCCAGGAGTGGACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGa                                                             >  1:1171360/1‑100 (MQ=255)

                               tACCGCCGCAGTCATCTGCCCAGGAGTGGACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGa                                                             >  2:1036597/1‑100 (MQ=255)

                                aCCGCCGCAGTCATCTGCCCAGGAGTGGACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGAt                                                            <  1:2979106/100‑1 (MQ=255)

                                 ccgccgCAGTCATCTGCCCAGGAGTGGACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATTAGACTGAACGTCATTCACTAACTGATa                                                           >  2:2104929/1‑100 (MQ=255)

                                   gccgcAGTCATCTGCCCAGGAGTGGACTCCAGAATCCGACTTCCGGTACAACCCGACAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACa                                                         >  1:1135140/1‑100 (MQ=255)

                                     cgcAGTCATCTGCCCAGGAGTGGACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACAtt                                                       >  1:2773597/1‑100 (MQ=255)

                                      gcAGTCATCTGCCCAGGAGTGGACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACAttt                                                      <  1:2697133/100‑1 (MQ=255)

                                       cAGTCATCTGCCCAGGAGTGGACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTg                                                     <  1:1257239/100‑1 (MQ=255)

                                       cAGTCATCTGCCCAGGAGTGGACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTg                                                     <  2:317648/100‑1 (MQ=255)

                                        aGTCATCTGCCCAGGCGTGGACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGc                                                    <  1:3063515/100‑1 (MQ=255)

                                         gTCATCTGCCCAGGAGTGGACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCt                                                   <  2:856122/100‑1 (MQ=255)

                                           cATCTGCCCAGGAGTGGACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCTcc                                                 <  2:1010855/100‑1 (MQ=255)

                                             tCTGCCCAGGAGTGGACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACCGTTGCTCcgc                                               >  1:617701/1‑100 (MQ=255)

                                               tGCCCAGGAGTGGACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCTCCgcgc                                             <  1:2363376/100‑1 (MQ=255)

                                               tGCCCAGGAGTGGACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCTCCgcgc                                             <  2:229861/100‑1 (MQ=255)

                                               tGCCCAGGAGTGGACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCTCCgcgc                                             <  2:1950842/100‑1 (MQ=255)

                                                gCCCAGGAGTGGACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCTCCGCGCt                                            >  2:440029/1‑100 (MQ=255)

                                                gCCCAGGAGTGGACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCTCCGCGCt                                            >  2:814867/1‑100 (MQ=255)

                                                gCCCAGGAGTGGACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCTCCGCGCt                                            >  2:2646878/1‑100 (MQ=255)

                                                 cccAGGAGTGGACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCTCCGCGCTg                                           <  1:1320771/100‑1 (MQ=255)

                                                  ccAGGAGTGGACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCTCCGCGCTGt                                          >  1:1898764/1‑100 (MQ=255)

                                                  ccAGGAGTGGACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCTCCGCCCTGt                                          >  2:2394775/1‑100 (MQ=255)

                                                    aGGAGTGGACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCTCCGCGCTGTAg                                        <  1:307335/100‑1 (MQ=255)

                                                     ggAGTGGACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCTCCGCGCTGTAgg                                       >  1:2147046/1‑100 (MQ=255)

                                                     ggAGTGGACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCTCCGCGCTGTAgg                                       <  1:2891196/100‑1 (MQ=255)

                                                     ggAGTGGACTCCAGAATCCGACTCCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCTCCGCGCTGTAgg                                       >  1:1088202/1‑100 (MQ=255)

                                                      gAGTGGACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCTCCGCGCTGTAggg                                      <  1:2790936/100‑1 (MQ=255)

                                                        gTGGACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCTCCGCGCTGTAGGGTa                                    >  1:19345/1‑100 (MQ=255)

                                                          ggACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCTCCGCGCTGTAGGGTAcc                                  >  1:411101/1‑100 (MQ=255)

                                                          ggACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCTCCGCGCTGTAGGGTAcc                                  >  1:520225/1‑100 (MQ=255)

                                                          ggACTCCAGAATCCGACGTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCTCCGCGCTGTAGGGTAcc                                  <  2:2707011/100‑1 (MQ=255)

                                                             cTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCTCCGCGCTGTAGGGTACCAGt                               >  1:1613754/1‑100 (MQ=255)

                                                             cTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCTCCGCGCTGTAGGGTACCAGt                               >  1:2930190/1‑100 (MQ=255)

                                                             cTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCTCCGCGCTGTAGGGTACCAGt                               >  1:2397612/1‑100 (MQ=255)

                                                             cTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCTCCGCGCTGTAGGGTACCAGt                               >  1:468772/1‑100 (MQ=255)

                                                             cTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCTCCGCGCTGTAGGGTACCAGt                               >  2:465256/1‑100 (MQ=255)

                                                               ccAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCTCCGCGCTGTAGGGTACCAGTGc                             >  1:1466532/1‑100 (MQ=255)

                                                               ccAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCTCCGCGCTGTAGGGTACCAGTGc                             <  1:2650324/100‑1 (MQ=255)

                                                                cAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCTCCGCGCTGTAGGGTACCAGTGCg                            >  2:1961278/1‑100 (MQ=255)

                                                                 agagTCCGACTTCCGGTACAACCCAGAAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCTCCGCGCTGTAGGGTACCAGTGCgg                           <  2:1009459/100‑1 (MQ=255)

                                                                   aaTCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCTCCGCGCTGTAGGGTACCAGTGCGGTc                         <  2:1621181/100‑1 (MQ=255)

                                                                     tCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCTCCGCGCTGTAGGGTACCAGTGCGGTCCg                       >  1:1711677/1‑100 (MQ=255)

                                                                       cGACTTCCGGTACAACCCGGCAAAAAAGGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCTCCGCGCTGTAGGGTACCAGTGCGGTCCGGc                     <  1:1714613/100‑1 (MQ=255)

                                                                         aCTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCTCCGCGCTGTAGGGTACCAGTGCGGTCCGGCTa                   >  1:506329/1‑100 (MQ=255)

                                                                         aCTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCTCCGCGCTGTAGGGTACCAGTGCGGTCCGGCTa                   <  2:1415768/100‑1 (MQ=255)

                                                                          cTTCCGGTACAACCCGGCCAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCTCCGCGCTGTAGGGTACCAGTGCGGTCCGGCTa                   >  1:1004518/1‑99 (MQ=255)

                                                                            tCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCTCCGCGCTGTAGGGTACCAGTGCGGTCCGGCTAATc                >  1:680605/1‑100 (MQ=255)

                                                                               ggTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCTCCGCGCTGTAGGGTACCAGTGCGGTCCGGCTAATCTGa             >  1:1949596/1‑100 (MQ=255)

                                                                               ggTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCTCCGCGCTGTAGGGTACCAGTGCGGTCCGGCTAATCTGa             <  2:2614609/100‑1 (MQ=255)

                                                                               ggTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCTCCGCGCTGTAGGGTACCAGTGCGGTCCGGCTAATCTGa             >  2:1546055/1‑100 (MQ=255)

                                                                               ggTACAACCCGGCAAAAAATGAGGATAAAACTGAACGTCATTCACTAACTGATACATTTGCTCCGCGCTGTAGGGTACCAGTGCGGTCCGGCTAATCTGa             >  2:3038656/1‑100 (MQ=255)

                                                                                gTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCTCCGCGCTGTAGGGTACCAGTGCGGTCCGGCTAATCTGAg            <  2:520223/100‑1 (MQ=255)

                                                                                 tACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCTCCGCGCTGTAGGGTACCAGTGCGGTCCGGCTAATCTGAgg           <  1:501437/100‑1 (MQ=255)

                                                                                 tACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCTCCGCGCTGTAGGGTACCAGTGCGGTCCGGCTAATCTGAgg           <  2:1922075/100‑1 (MQ=255)

                                                                                 tACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCTCCGCGCTGTAGGGTACCAGTGCGGTCCGGCTAATCTGAgg           <  2:2148486/100‑1 (MQ=255)

                                                                                 tACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCTCCGCGCTGTAGGGTACCAGTGCGGTCCGGCTAATCTGAgg           >  2:3047166/1‑100 (MQ=255)

                                                                                  ataaCCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCTCCGCGCTGTAGGGTACCAGTGCGGTCCGGCTAATCTGAGGc          <  2:1881679/98‑1 (MQ=255)

                                                                                  acaaacaaacAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCTCCGCGCTGTAGGGTACCAGTGCGGTCCGGCTAATCTGAgg           <  2:742557/90‑1 (MQ=255)

                                                                                  aCAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCTCCGCGCTGTAGGGTACCAGTGCGGTCCGGCTAATCTGAGGc          <  2:368901/100‑1 (MQ=255)

                                                                                   cAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCTCCGCGCTGTAGGGTACCAGTGCGGTCCGGCTAATCTGAGGCa         >  1:2233203/1‑100 (MQ=255)

                                                                                   cAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCTCCGCGCTGTAGGGTACCAGTGCGGTCCGGCTAATCTGAGGCa         <  1:1766075/100‑1 (MQ=255)

                                                                                      cccGGCAAAACATGAGGATACGCCTCAACGTCATTCACTAACTGATACATTTGCTCCGCGCTGCAGGGTACCAGTGCGGTCCGGCTAATCTGACGCACaa      >  2:242589/1‑100 (MQ=255)

                                                                                      cccGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCTCCGCGCTGTAGGGTACCAGTGCGGTCCGGCTAATCTGAGGCATaa      >  1:380326/1‑100 (MQ=255)

                                                                                          gCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCTCCGCGCTGTAGGGTACCAGTGCGGTCCGGCTAATCTGAGGCATAACAAt  >  2:1568723/1‑100 (MQ=255)

ACGTTTTGCTGATCCCAGCCTTAGAGACATCTACCGCCGCAGTCATCTGCCCAGGAGTGGACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAACTGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCTCCGCGCTGTAGGGTACCAGTGCGGTCCGGCTAATCTGAGGCATAACAAT  >  NC_000913/2754542‑2754731

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`