Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,705,985 T→C 25.6% intergenic (‑195/+113) dppB ← / ← dppA dipeptide/heme ABC transporter permease/dipeptide/heme ABC transporter periplasmic binding protein; dipeptide chemotaxis receptor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,705,9850TC25.6% 324.3 / 116.8 168intergenic (‑195/+113)dppB/dppAdipeptide/heme ABC transporter permease/dipeptide/heme ABC transporter periplasmic binding protein; dipeptide chemotaxis receptor
Reads supporting (aligned to +/- strand):  ref base T (62/63);  new base C (41/2);  total (103/65)
Fisher's exact test for biased strand distribution p-value = 1.35e-08
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.59e-01

Only 100 of 169 total aligned reads displayed.

GCTGCCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTCGTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCTTTGCCATCAGTCTT  >  NC_000913/3705887‑3706084
                                                                                                   |                                                                                                       
gctgcCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTa                                                                                                        >  2:2002788/1‑100 (MQ=255)
gctgcCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTa                                                                                                        <  1:1498460/100‑1 (MQ=255)
  tgcCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATc                                                                                                      >  2:2136119/1‑100 (MQ=255)
  tgcCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATc                                                                                                      >  1:696283/1‑100 (MQ=255)
  tgcCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATc                                                                                                      <  2:566474/100‑1 (MQ=255)
    ccGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCg                                                                                                    <  1:2852104/100‑1 (MQ=255)
     cGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCgg                                                                                                   <  1:766363/100‑1 (MQ=255)
      ggATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGc                                                                                                  <  1:2622439/100‑1 (MQ=255)
       gATGGCGCTGCACGCAGAGGCACAGCGTCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGcc                                                                                                 <  2:2517275/100‑1 (MQ=255)
       gATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGcc                                                                                                 <  2:180362/100‑1 (MQ=255)
       gATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATAAGGCGTTAACGCCGCATCCGGca                                                                                                 <  1:999751/100‑2 (MQ=37)
        aTGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCt                                                                                                <  2:2245566/100‑1 (MQ=255)
           gcgcTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACa                                                                                            <  1:496771/100‑1 (MQ=255)
            cgcTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACaa                                                                                           >  1:273572/1‑100 (MQ=255)
             gcTTCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGAATGCGGCGTTAACGCCTTATCCGGCCTACaaa                                                                                          <  2:724490/100‑1 (MQ=255)
             gcTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACaaa                                                                                          >  2:1431338/1‑100 (MQ=255)
              cTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACaaaa                                                                                         >  1:2132347/1‑100 (MQ=255)
                gCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATc                                                                                       >  1:1004754/1‑100 (MQ=255)
                   cGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTg                                                                                    >  1:550044/1‑100 (MQ=255)
                   cGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTg                                                                                    >  2:1334618/1‑100 (MQ=255)
                    gCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGTCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGc                                                                                   <  2:1370391/100‑1 (MQ=255)
                       gagGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCaaa                                                                                >  1:40829/1‑100 (MQ=255)
                       gagGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCGTTGTTTATGCCGGATACGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCaaa                                                                                >  2:2998643/1‑100 (MQ=255)
                         ggCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAAtt                                                                              >  1:1995156/1‑100 (MQ=255)
                          gCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTc                                                                             <  1:2828116/100‑1 (MQ=255)
                           cacaGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCa                                                                            >  2:269383/1‑100 (MQ=255)
                           cacaGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCa                                                                            >  2:2913057/1‑100 (MQ=255)
                            gcaGCGCCATCCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCa                                                                            <  2:2222331/99‑1 (MQ=255)
                              aGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATa                                                                         <  2:93137/100‑1 (MQ=255)
                               gcgcCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATAc                                                                        <  1:2661750/100‑1 (MQ=255)
                               gcgcCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATAc                                                                        >  1:2709870/1‑100 (MQ=255)
                               gcgcCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATAc                                                                        >  1:2862786/1‑100 (MQ=255)
                                 gcCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCGTATCCGGCCTACAAAATCGTGCAAATTCAATACAt                                                                      >  2:2413418/1‑100 (MQ=255)
                                  ccACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACAtt                                                                     <  1:147234/100‑1 (MQ=255)
                                   cACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTg                                                                    <  1:672864/100‑1 (MQ=255)
                                       cGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAg                                                                >  1:2685563/1‑100 (MQ=255)
                                        gggTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGa                                                               <  2:3058533/100‑1 (MQ=255)
                                          gTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAAt                                                            <  2:2373464/100‑1 (MQ=255)
                                           tataCAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAAtt                                                          >  1:1843147/1‑100 (MQ=255)
                                                aGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTCGt                                                     >  2:829813/1‑100 (MQ=255)
                                                 gATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTCGTa                                                    <  1:2061772/100‑1 (MQ=255)
                                                   ttgttgACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGCATTTTTGTAgg                                                  >  1:1179582/1‑100 (MQ=255)
                                                     gttgACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAAttt                                                         >  2:2555142/1‑91 (MQ=255)
                                                       tgACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCGTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTCGTAGGCCt                                               >  2:767657/1‑99 (MQ=255)
                                                       tgACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTCGTAGGCCTg                                              >  2:1876444/1‑100 (MQ=255)
                                                       tgACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTCGTAGGCCTg                                              >  1:2236491/1‑100 (MQ=255)
                                                       tgACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCGTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGTATTTTCGTAGGCCt                                               >  2:1258312/1‑99 (MQ=255)
                                                        gccAAAGTGCGCTTCGTTTATGCCGGATGCGGAGTTAACGCCTTATCGGGCCCGCAAAATCGTGCCAGTTCCTTCCATTGCAGCCTTTTCATCGGcc                                                >  2:2754636/3‑97 (MQ=9)
                                                         aCAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTCGTAGGCCTGAt                                            >  2:1932048/1‑100 (MQ=255)
                                                                 cgcTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTCGTAGGCCTGATAAGCGGAg                                    <  1:2396036/100‑1 (MQ=255)
                                                                  gcTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTCGTAGGCCTGATAAGCGGAgc                                   <  2:791689/100‑1 (MQ=255)
                                                                   cTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTCGTAGGCCTGATAAGCGGAgcg                                  <  2:116488/100‑1 (MQ=255)
                                                                    tttGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTCGTAGGCCTGATAAGCGGAgcgc                                 >  2:566416/1‑100 (MQ=255)
                                                                     ttGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTCGTAGGCCTGATAAGCGGAGCGCa                                <  1:2076635/100‑1 (MQ=255)
                                                                      tttgtaggCCGGATAAGGCGTTTACGCCGCATCCGGCCTACAAAAGCATGCAAATTCAATATATTGCAGAGTTCACGTAGGCCTGATAAGCGTAGCGCAt                               >  1:114102/8‑100 (MQ=17)
                                                                      cttgtaggCCGGATAAGGCGTTCACGCCGCATCCGGCATACAAGTTCGTGCAAATTCAATAAATTGCAATATGACGTAGGCCTGATAAGCGTAGCGCATc                              >  1:471439/8‑100 (MQ=18)
                                                                       ttgtaggCCGGATAAGGCGTTTACGCCGCATCCGGCCTACAAATTCTGCAAATTCAACAAATTGCGAATCCCTTGTAGGCCTGATAAGCGTAGCGCATCa                             >  1:703553/7‑100 (MQ=18)
                                                                       gTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTCGTAGGCCTGATAAGCGGAGCGCATc                              <  2:2253477/100‑1 (MQ=255)
                                                                         ttATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTCGTAGGCCTGATAAGCGGAGCGCATCAg                            <  2:2419844/100‑1 (MQ=255)
                                                                         ttATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTCGTAGGCCTGATAAGCGGAGCGCATCAg                            <  1:895449/100‑1 (MQ=255)
                                                                         gtaggCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAAGTCGTATAAATTCAATGTATTGCAGTTTATTGGTAGGCCTGATAAGCGCAGCGCATCAg                            >  1:538435/5‑100 (MQ=21)
                                                                         gtaggCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAAATCGCTTAAATTCAATATATTGCACCATCCTCGTAGGCCTGATAAGCGTAGCGCATCAg                            >  2:2800360/5‑100 (MQ=21)
                                                                         gtaggCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAAAGCATGAAAATTCAATATATTGCAGGAGCTGCGTAGGCCTGATAAGCGTAGCGCATCAg                            >  1:1050866/5‑100 (MQ=17)
                                                                         gtaggCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAAAACCTGCAAATTCAATATATTGCAGGATACGTGTAGGCCTGATAAGCGTAGCGCATCAg                            >  1:1975596/5‑100 (MQ=18)
                                                                         gtaggCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAAAACATGCAAATTCAATAGATTGCAGAGATTATGTAGGCCTGATAAGCGTAGCGCATCAg                            >  2:1903096/5‑100 (MQ=17)
                                                                         gtaggCCGGATAAGGCGTTAACGCCGCATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTCGTAGGCCTGATAAGCGGAGCGCATCAg                            >  2:705336/5‑100 (MQ=18)
                                                                          tATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTCGTAGGCCTGATAAGCGGAGCGCATCAgg                           >  2:322928/1‑100 (MQ=255)
                                                                           aggCCGGATAAGGCGTTAACGCCGCATCCGGCATACAAAATCGTGCAAATTCAATACATTGCAGAATTTTCGTAGGCCTGATAAGCAGAACGCATCa                             >  1:1765018/3‑97 (MQ=17)
                                                                           aTGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTCGTAGGCCTGATAAGCGGAGCGCATCAGGc                          >  1:702360/1‑100 (MQ=255)
                                                                            ggCCGGATAAGGCGTTTACGCCGCATCCGGCCTACAAAAGCATGCAAATTCAATATATTGCAGAGTTCACGTAGGCCTGATAAGCGTAGCGCATCAGGCa                         >  2:50535/2‑100 (MQ=17)
                                                                            ggCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAAATCATGAAAATTCAATACATTGCAAGATTTTCGTAGGCCTGATAAGCGTAGCGCATCAGGCa                         >  1:833803/2‑100 (MQ=17)
                                                                            ggCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAAACTTTGCAAATTCAATATATTGCAATCTCCGTGTAGGCCTGATAAGCGTAGCGCATCAGGCa                         >  1:2478660/2‑100 (MQ=18)
                                                                            ggCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAAACCTTGCTAATTCAATATATTGCAGGGACTATGTAGGCCTGATAAGCATAGCGCATCAGGCa                         >  1:2474904/2‑100 (MQ=18)
                                                                            ggCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAAAACATGCAAATTCAATAGATTGCAGAGATTATGTAGGCCTGATAAGCGTAGCGCATCAGGCa                         >  1:1845318/2‑100 (MQ=17)
                                                                            ggCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAATCATGCAAATCCAAAATATTGCAGGAGTTATGTAGGCCTGATAAGCGTAGCGCATCAGGCAt                        >  1:2289011/2‑100 (MQ=21)
                                                                             gCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTCGTAGGCCTGATAAGCGGAGCGCATCAGGCAt                        >  2:62791/1‑100 (MQ=255)
                                                                             gCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTCGTAGGCCTGATAAGCGGAGCGCATCAGGCAt                        >  2:1271822/1‑100 (MQ=255)
                                                                             gCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTCGTAGGCCTGATAAGCGGAGCGCATCAGGCAt                        >  1:1588256/1‑100 (MQ=255)
                                                                             gCCGGATAAGGCGTTTACGCCGCATCCGGCCTACAAAAGCATGCAAATTCAATATATTGCAGAGTTCACGTAGGCCTGATAAGCGTAGCGCATCAGGCag                        >  2:536358/1‑99 (MQ=17)
                                                                             gCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAAGTCGTATAAATTCAATGTATTGCAGTTTATTGGTAGGCCTGATAAGCGCAGCGCATCAGGCaa                        >  1:1248746/1‑99 (MQ=25)
                                                                              ccggataaggcgTTCACGCCGCATCCGGCCTACAAAATCGCTTAAATTCAATATATTGCACCATCCTCGTAGGCCTGATAAGCGTAGCGCATCAGGCaat                       >  2:1002304/9‑98 (MQ=21)
                                                                              ccggataaggcgTTCACGCCGCATCCGGCCTACAAAAGCACGCAAACTCAATATATTGCAGAGATCATGTAGGCCTGATAAGCGTAGCGCATCAGGCaat                       >  2:2864190/9‑98 (MQ=17)
                                                                              ccGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTCGTAGGCCTGATAAGCGGAGCGCATCAGGccta                       <  2:503303/100‑4 (MQ=255)
                                                                                ggataaggcgTTTACGCCGCATCCGGCCTACACATTCTGCAAATTCAACAAATTGCGAATCCCTTGTAGGCCTGATAAGCGTAGCGCATCAGGCAtttgc                    >  1:2944336/7‑98 (MQ=18)
                                                                                ggataaggcgTTTACGCCGCATCCGGCCTACAAAACAGTGCAAATTCAATATATTGCAATCTTCTGGTAGGCCTGATAAGCATCGCGCATCAGGCaagct                     >  2:2666410/7‑96 (MQ=21)
                                                                                  aTGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTCGTAGGCCTGATAAGCGGAGCGCATCAGGCATTtttg                   <  1:1431348/100‑1 (MQ=255)
                                                                                   taaggcgTTCACGCCGCATCCGGCCTACAAAAATCTTGCCAATTCAATATATTGCAGGACCATGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTtttgc                  >  1:1566176/4‑100 (MQ=18)
                                                                                   taaggcgTTCACGCCGCATCCGGCCTACAAAGTCGTATAAATTCAATGTATTGCAGTTTATTGGTAGGCCTGATAAGCGCAGCGCATCAGGCaatcttac                  >  2:1281967/4‑93 (MQ=25)
                                                                                   tGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTCGTAGGCCTGATAAGCGGAGCGCATCAGGCATTtttgc                  >  1:571194/1‑100 (MQ=255)
                                                                                    gcggcgTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTCGTAGGCCTGATAAGCGGAGCGCATCAGGCATTtttgcc                 >  1:818485/1‑100 (MQ=255)
                                                                                     cggcgTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTCGTAGGCCTGATAAGCGGAGCGCATCAGGCATTtttgcct                >  2:2467307/1‑100 (MQ=255)
                                                                                     aggcgTTCACGCCGCATCCGGCCTACAAAACCTTGCTAATTCAATATATTGCAGGGACTATGTAGGCCTGATAAGCATAGCGCATCAGGCagctttacgt                >  1:1349939/2‑91 (MQ=21)
                                                                                        cgTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTCGTAGGCCTGATAAGCGGAGCGCATCAGGCATTtttgcctttn             <  2:1755328/100‑2 (MQ=255)
                                                                                        cgTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTCGTAGGCCTGATAAGCGGAGCGCATCAGGCATTtttgcctttg             <  1:570961/100‑1 (MQ=255)
                                                                                         gTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTCGTAGGCCTGATAAGCGGAGCGCATCAGGCATTtttgcctttgc            <  1:322930/100‑1 (MQ=255)
                                                                                              cGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTCGTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGTCTTTGCCATc        >  2:2061358/1‑99 (MQ=255)
                                                                                               gCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTCGTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCTTTGCCATCAg      >  2:89427/1‑100 (MQ=255)
                                                                                                ccTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTCGTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCTTTGCCAtagat     >  1:3072196/1‑96 (MQ=255)
                                                                                                 cTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTCGTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCTTTGCCATCAGTc    <  2:1476792/100‑1 (MQ=255)
                                                                                                   tATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTCGTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCTTTGCCATCAGTCtt  <  1:2946207/100‑1 (MQ=255)
                                                                                                   |                                                                                                       
GCTGCCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTCGTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCTTTGCCATCAGTCTT  >  NC_000913/3705887‑3706084

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: