BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,433,040	G→C	P296R (CCG→CGG)	accD ←	acetyl‑CoA carboxylase, beta (carboxyltransferase) subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,433,040	0	G	C	100.0%	572.4 / NA	152	P296R (CCG→CGG)	accD	acetyl‑CoA carboxylase, beta (carboxyltransferase) subunit
Reads supporting (aligned to +/- strand): ref base G (0/0); new base C (75/77); total (75/77)

Only 100 of 157 total aligned reads displayed.

GGTATCCGCTGATTCGTTACGGTGAGAATAGCAAAAGGGCAGAGCCAGTGGCCCTGCCCTTATCAGTTATCAGGCCTCAGGTTCCTGATCCGGTACCGGGGGTACCACTACGCCTTCACGCGGCGCTTCAGGATTCGGCGCTGGCAGATTCATCAACTTCGCCAGAATGCTCGCCAGTTTCAGGCGCATTTCCG  >  NC_000913/2432943‑2433136

ggTATCCGCTGATTCGTTACGGTGAGAATAGCAAAAGGGCAGAGCCAGTGGCCCTGCCCTTATCAGTTATCAGGCCTCAGGTTCCTGATCCGGTACCcgg                                                                                                <  1:762131/100‑1 (MQ=255)

 gTATCCGCTGATTCGTTACGGTGAGAATAGCAAAAGGGCAGAGCCAGTGGCCCTGCCCTTATCAGTTATCAGGCCTCAGGTTCCTGATCCGGTACCcggg                                                                                               <  1:3453964/100‑1 (MQ=255)

 gTATCCGCTGATTCGTTACGGTGAGAATAGCAAAAGGGCAGAGCCAGTGGCCCTGCCCTTATCAGTTATCAGGCCTCAGGTTCCTGATCCGGTACCcggg                                                                                               >  1:2792597/1‑100 (MQ=255)

  tATCCGCTGATTCGTTACGGTGAGAATAGCAAAAGGGCAGAGCCAGTGGCCCTGCCCTTATCAGTTATCAGGCCTCAGGTTCCTGATCCGGTACCcgggg                                                                                              <  2:2403318/100‑1 (MQ=255)

     ccGCTGATTCGTTACGGTGAGAATAGCAAAAGGGCAGAGCCAGTGGCCCTGCCCTTATCAGTTATCAGGCCTCAGGTTCCTGATCCGGTACCCGGGGTAc                                                                                           >  2:3646692/1‑100 (MQ=255)

      cGCTGATTCGTTACGGTGAGAATAGCAAAAGGGCAGAGCCAGTGGCCCTGCCCTTATCAGTTATCAGGCCTCAGGTTCCTGATCCGGTACCCGGGGTAcc                                                                                          <  1:3264178/100‑1 (MQ=255)

      cGCTGATTCGTTACGGTGAGAATAGCAAAAGGGCAGAGCCAGTGGCCCTGCCCTTATCAGTTATCAGGCCTCAGGTTCCTGATCCGGTACCCGGGGTAcc                                                                                          <  1:1831163/100‑1 (MQ=255)

      cGCTGATTCGTTACGGTGAGAATAGCAAAAGGGCAGAGCCAGTGGCCCTGCCCTTATCAGTTATCAGGCCTCAGGTTCCTGATCCGGTACCCGGGGTAcc                                                                                          >  2:1013743/1‑100 (MQ=255)

      cGCTGATTCGTTACGGTGAGAATAGCAAAAGGGCAGAGCCAGTGGCCCTGCCCTTATCAGTTATCAGGCCTCAGGTTCCTGATCCGGTACCCGGGGTAcc                                                                                          >  2:1069268/1‑100 (MQ=255)

        cTGATTCGTTACGGTGAGAATAGCAAAAGGGCAGAGCCAGTGGCCCTGCCCTTATCAGTTATCAGGCCTCAGGTTCCTGATCCGGTACCCGGGGTACCAc                                                                                        <  1:3562059/100‑1 (MQ=255)

        cTGATTCGTTACGGTGAGAATAGCAAAAGGGCAGAGCCAGTGGCCCTGCCCTTATCAGTTATCAGGCCTCAGGTTCCTGATCCGGTACACGGGGTACCAc                                                                                        >  1:1863601/1‑100 (MQ=255)

          gATTCGTTACGGTGAGAATAGCAAAAGGGCAGAGCCAGTGGCCCTGCCCTTATCAGTTATCAGGCCTCAGGTTCCTGATCCGGTACCCGGGGTACCACTa                                                                                      >  1:643208/1‑100 (MQ=255)

          gATTCGTTACGGTGAGAATAGCAAAAGGGCAGAGCCAGTGGCCCTGCCCTTATCAGTTATCAGGCCTCAGGTTCCTGATCCGGTACCCGGGGTACCACTa                                                                                      >  1:187036/1‑100 (MQ=255)

           aTTCGTTACGGTGAGAATAGCAAAAGGGCAGAGCCAGTGGCCCTGCCCTTATCAGTTATCAGGCCTCAGGTTCCTGATCCGGTACCCGGGGTACCACTAc                                                                                     <  2:3415478/100‑1 (MQ=255)

            ttCGTTACGGTGAGAATAGCAAAAGGGCAGAGCCAGTGGCCCTGCCCTTATCAGTTATCAGGCCTCAGGTTCCTGATCCGGTACCCGGGGTACCACTACg                                                                                    >  1:377350/1‑100 (MQ=255)

              cGTTACGGTGAGAATAGCAAAAGGGCAGAGCCAGTGGCCCTGCCCTTATCAGTTATCAGGCCTCAGGTTCCTGATCCGGTACCCGGGGTACCACTACGcc                                                                                  <  2:963487/100‑1 (MQ=255)

               gTTACGGTGAGAATAGCAAAAGGGCAGAGCCAGTGGCCCTGCCCTTATCAGTTATCAGGCCTCAGGTTCCTGATCCGGTACCCGGGGTACCACTACGCCt                                                                                 >  1:837872/1‑100 (MQ=255)

               gTTACGGTGAGAATAGCAAAAGGGCAGAGCCAGTGGCCCTGCCCTTATCAGTTATCAGGCCTCAGGTTCCTGATCCGGTACCCGGGGTACCACTACGCCt                                                                                 >  1:2854585/1‑100 (MQ=255)

                  aCGGTGAGAATAGCAAAAGGGCAGAGCCAGTGGCCCTGCCCTTATCAGTTATCAGGCCTCAGGTTCCTGATCCGGTACCCGGGGTACCACTACGCCTTCa                                                                              >  2:3658658/1‑100 (MQ=255)

                  aCGGTGAGAATAGCAAAAGGGCAGAGCCAGTGGCCCTGCCCTTATCAGTTATCAGGCCTCAGGTTCCTGATCCGGTACCCGGGGTACCACTACGACTTCa                                                                              <  2:527510/100‑1 (MQ=255)

                   cGGTGAGAATAGCAAAAGGGCAGAGCCAGTGGCCCTGCCCTTATCAGTTATCAGGCCTCAGGTTCCTGATCCGGTACCCGGGGTACCACTACGCCTTCAc                                                                             <  2:1374400/100‑1 (MQ=255)

                    ggTGAGAATAGCAAAAGGGCAGAGCCAGTGGCCCTGCCCTTATCAGTTATCAGGCCTCAGGTTCCTGATCCGGTACCCGGGGTACCACTACGCCTTCAcg                                                                            >  2:1650173/1‑100 (MQ=255)

                     gTGAGAATAGCAAAAGGGCAGAGCCAGTGGCCCTGCCCTTATCAGTTATCAGGCCTCAGGTTCCTGATCCGGTACCCGGGGTACCACTACGCCTTCAcgc                                                                           >  1:2640583/1‑100 (MQ=255)

                     gTGAGAATAGCAAAAGGGCAGAGCCAGTGGCCCTGCCCTTATCAGTTATCAGGCCTCAGGTTCCTGATCCGGTACCCGGGGTACCACTACGCCTTCAcgc                                                                           >  1:1282100/1‑100 (MQ=255)

                      tGAGAATAGCAAAAGGGCAGAGCCAGTGGCCCTGCCCTTATCAGTTATCAGGCCTCAGGTTCCTGATCCGGTACCCGGGGTACCACTACGCCTTCAcgcg                                                                          <  1:2658499/100‑1 (MQ=255)

                        agaATAGCAAAAGGGCAGAGCCAGTGGCCCTGCCCTTATCAGTTATCAGGCCTCAGGTTCCTGATCCGGTACCCGGGGTACCACTACGCCTTCAcgcgac                                                                        <  1:1860479/100‑3 (MQ=255)

                            tAGCAAAAGGGCAGAGCCAGTGGCCCTGCCCTTATCAGTTATCAGGCCTCAGGTTCCTGATCCGGTACCCGGGGTACCACTACGCCTTCACGCGGCGCtt                                                                    >  2:2900652/1‑100 (MQ=255)

                            tAGCAAAAGGGCAGAGCCAGTGGCCCTGCCCTTATCAGTTATCAGGCCTCAGGTTCCTGATCCGGTACCCGGGGTACCACTACGCCTTCACGCGGCGCtt                                                                    >  2:8504/1‑100 (MQ=255)

                             ctgataaGGGCAGGGCCACTGGCTCTGCCCTTATCAGTTATCAGGCCTCAGGTTCCTGATCCGGTACCCGGGGTACCACTACGCCTTCACGCGGCGCTTc                                                                   >  1:2772827/6‑100 (MQ=37)

                             aGCAAAAGGGCAGAGCCAGTGGCCCTGCCCTTATCAGTTATCAGGCCTCAGGTTCCTGATCCGGTACCCGGGGTACCACTACGCCTTCACGCGGCGCTTc                                                                   >  1:902910/1‑100 (MQ=255)

                              gCAAAAGGGCAGAGCCAGTGGCCCTGCCCTTATCAGTTATCAGGCCTCAGGTTCCTGATCCGGTACCCGGGGTACCACTACGCCTTCACGCGGCGCTTCa                                                                  >  2:235986/1‑100 (MQ=255)

                               cAAAAGGGCAGAGCCAGTGGCCCTGCCCTTATCAGTTATCAGGCCTCAGGTTCCTGATCCGGTACCCGGGGTACCACTACGCCTTCACGCGGCGCTTCAg                                                                 <  1:3314540/100‑1 (MQ=255)

                               cAAAAGGGCAGAGCCAGTGGCCCTGCCCTTATCAGTTATCAGGCCTCAGGTTCCTGATCCGGTACCCGGGGTACCACTACGCCTTCACGCGGCGCTTCAg                                                                 <  1:1979199/100‑1 (MQ=255)

                               cAAAAGGGCAGAGCCAGTGGCCCTGCCCTTATCAGTTATCAGGCCTCAGGTTCCTGATCCGGTACCCGGGGTACCACTACGCCTTCACGCGGCGCTTCAg                                                                 >  1:3390210/1‑100 (MQ=255)

                               cAAAAGGGCAGAGCCAGTGGCCCTGCCCTTATCAGTTATCAGGCCTCAGGTTCCTGATCCGGTACCCGGGGTACCACTACGCCTTCACGCGGCGCTTCAg                                                                 <  1:2966406/100‑1 (MQ=255)

                                aaaaGGGCAGAGCCAGTGGCCCTGCCCTTATCTGTTATCAGGACTCAGGTTCCTGATCCGGTACCCGGGGTACCACTACGCCTTCACGCGGCGCTTCAgg                                                                <  2:2718957/100‑1 (MQ=255)

                                aaaaGGGCAGAGCCAGTGGCCCTGCCCTTATCAGTTATCAGGCCTCAGGTTCCTGATCCGGTACCCGGGGTACCACTACGCCTTCACGCGGCGCTTCAgg                                                                <  1:1184041/100‑1 (MQ=255)

                                  aaGGGCAGAGCCAGTGGCCCTGCCCTTATCAGTTATCAGGCCTCAGGTTCCTGATCCGGTACCCGGGGTACCACTACGCCTTCACGCGGCGCTTCAGGAt                                                              <  1:3646709/100‑1 (MQ=255)

                                    gggCAGGGCCACTGGCTCTGCCCTTTTCAGTTATCAGGCCTCAGGTTCCTGATCCGGTACCCGGGGTACCACTACGCCTTCACGCGGCGCTTCAGGATTc                                                            >  1:1084952/1‑100 (MQ=38)

                                    gggCAGAGCCAGTGGCCCTGCCCTTATCAGTTATCAGGCCTCAGGTTCCTGATCCGGTACCCGGGGTACCACTACGCCTTCACGCGGCGCTTCAGGATTc                                                            <  1:302127/100‑1 (MQ=255)

                                    gggCAGAGCCAGTGGCCCTGCCCTTATCAGTTATCAGGCCTCAGGTTCCTGATCCGGTACCCGGGGTACCACTACGCCTTCACGCGGCGCTACAGGATTc                                                            >  1:1615821/1‑100 (MQ=255)

                                       cAGAGCCAGTGGCCCTGCCCTTATCAGTTATCAGGCCTCAGGTTCCTGATCCGGTACCCGGGGTACCACTACGCCTTCACGCGGCGCTTCAGGATTCGgc                                                         >  2:3117114/1‑100 (MQ=255)

                                        agagCCAGTGGCCCTGCCCTTATCAGTTATCAGGCCTCAGGTTCCTGATCCGGTACGCGGGGTACCACTACGCCTTCACGCGGCGCTTCAGGATTCGgcg                                                        >  2:2471317/1‑100 (MQ=255)

                                         gagCCAGTGGCCCTGCCCTTATCAGTTATCAGGCCTCAGGTTCCTGATCCGGTACCCGGGGTACCACTACGCCTTCACGCGGCGCTTCAGGATTCGgcg                                                        <  1:3363103/99‑1 (MQ=255)

                                          agCAAGTGGCCCTGCCCTTATCAGTTATCAGGCCTCAGGTTCCTGATCCGGTACCCGGGGTACCACTACGCCTTCACCCGGCGCTTCAGGATTCGGCGCt                                                      <  2:2254820/100‑1 (MQ=255)

                                           gCCAGTGGCCCTGCCCTTATCAGTTATCAGGCCTCAGGTTCCTGATCCGGTACCCGGGGTACCACTACGCCTTCACGCGGCGCTTCAGGATTCGGCGCTg                                                     >  1:1062312/1‑100 (MQ=255)

                                            ccAGTGGCCCTGCCCTTATCAGTTATCAGGCCTCAGGTTCCTGATCCGGTACCCGGGGTACCACTACGCCTTCACGCGGCGCTTCAGGATTCGGCGCTgg                                                    <  1:409069/100‑1 (MQ=255)

                                               gTGGCCCTGCCCTTATCAGTTATCAGGCCTCAGGTTCCTGATCCGGTACCCGGGGTACCACTACGCCTTCACGCGGCGCTTCAGGATTCGGCGCTGGCAg                                                 <  2:2875144/100‑1 (MQ=255)

                                                 ggCCCTGCCCTTATCAGTTATCAGGCCTCAGGTTCCTGATCCGGTACCCGGGGTACCACTACGCCTTCACGCGGCGCTTCAGGATTCGGCGCTGGCAGAt                                               <  2:428981/100‑1 (MQ=255)

                                                 ggCCCTGCCCTTATCAGTTATCAGGCCTCAGGTTCCTAATCCGGTACCCGGGGTACCACTACGCCTTCACGCGGCGCTTCAGGATTCGGCGCTGGCAGAt                                               <  2:15403/100‑1 (MQ=255)

                                                  gccctgccctTATCAGTTATCAGGCCTCAGGTTCCTGATCCGGTACCCGGGGTACCACTACGCCTTCACGCGGCGCTTCAGGATTCGGCGCTGGCAGAtt                                              <  2:392207/100‑1 (MQ=255)

                                                   ccctgccctTATCAGTTATCAGGCCTCAGGTTCCTGATCCGGTACCCGGGGTACCACTACGCCTTCACGCGGCGCTTCAGGATTCGGCGCTGGCAGATtc                                             >  2:2573520/1‑100 (MQ=255)

                                                   ccctgccctTATCAGTTATCAGGCCTCAGGTTCCTGATCCGGTACCCGGGGTACCACTACGCCTTCACGCGGCGCTACAGGATTCGGCGCTGGCAGATtc                                             <  2:1615817/100‑1 (MQ=255)

                                                    cctgccctTATCAGTTATCAGGCCTCAGGTTCCTGATCCGGTACCCGGGGTACCACTACGCCTTCACGCGGCGCTTCAGGATTCGGCGCTGGCAGATtca                                            >  1:2896346/1‑100 (MQ=255)

                                                    cctgccctTATCAGTTATCAGGCCTCAGGTTCCTGATCCGGTACCCGGGGTACCACTACGCCTTCACGCGGCGCTTCAGGATTCGGCGCTGGCAGATtca                                            >  1:2095458/1‑100 (MQ=255)

                                                      tgccctTATCAGTTATCAGGCCTCAGGTTCCTGATCCGGTACCCGGGGTACCACTACGCCTTCACGCGGCGCTTCAGGATTCGGCGCTGGCAGATtcatc                                          <  2:1904026/100‑1 (MQ=255)

                                                       gccctTATCAGTTATCAGGCCTCAGGTTCCTGATCCGGTACCCGGGGTACCACTACGCCTTCACGCGGCGCTTCAGGATTCGGCGCTGGCAGATtcatca                                         <  2:2740823/100‑1 (MQ=255)

                                                       gccctTATCAGTTATCAGGCCTCAGGTTCCTGATCCGGTACCCGGGGTACCACTACGCCTTCACGCGGCGCTTCAGGATTCGGCGCTGGCAGATtcatca                                         <  2:3390193/100‑1 (MQ=255)

                                                       gccctTATCAGTTATCAGGCCTCAGGTTCCTGATCCGGTACCCGGGGTACCACTACGCCTTCACGCGGCGCTTCAGGATTCGGCGCTGGCAGATtcatca                                         <  1:3768511/100‑1 (MQ=255)

                                                         cctTATCAGTTATCAGGCCTCAGGTTCCTGATCCGGTACCCGGGGTACCACTACGCCTTCACGCGGCGCTTCAGGATTCGGCGCTGGCAGATTCATCAAc                                       >  2:720942/1‑100 (MQ=255)

                                                          ctTATCAGTTATCAGGCCTCAGGTTCCTGATGCGGTACCCGGGGTACCACTACGCCTTCACGCGGCGCTTCAGGATTCGGCGCTGGCAGATTCATCAACt                                      >  1:2372523/1‑100 (MQ=255)

                                                          ctTATCAGTTATCAGGCCTCAGGTTCCTGATCCGGTACCCGGGGTACCACTACGCCTTCACGCGGCGCTTCAGGATTCGGCGCTGGCAGATTCATCAACt                                      <  1:1535729/100‑1 (MQ=255)

                                                            tatcagttatcagGCCTCAGGTTCCTGATCCGGTACCCGGGGTACCACTACGCCTTCACGCGGCGCTTCAGGATTCGGCGCTGGCAGATTCATCAACTTc                                    >  1:3156371/1‑100 (MQ=255)

                                                               cagttatcagGCCTCAGGTTCCTGATCCGGTACCCGGGGTACCACTACGCCTTCACGCGGCGCTTCAGGATTCGGCGCTGGCAGATTCATCAACTTCGcc                                 <  1:167894/100‑1 (MQ=255)

                                                               cagttatcagGCCTCAGGTTCCTGATCCGGTACCCGGGGTACCACTACGCCTTCACGCGGCGCTTCAGGATTCGGCGCTGGCAGATTCATCAACTTCGcc                                 <  2:1243089/100‑1 (MQ=255)

                                                               cagttatcagGCCTCAGGTTCCTGATCCGGTACCCGGGGTACCACTACGCCTTCACGCGGCGCTTCAGGATTCGGCGCTGGCAGATTCATCAACTTCGcc                                 <  2:3422513/100‑1 (MQ=255)

                                                                agttatcagGCCTCAGGTTCCTGATCCGGTACCCGGGGTACCACTACGCCTTCACGCGGCGCTTCAGGATTCGGCGCTGGCAGATTCATCAACTTCGCCa                                >  2:1467132/1‑100 (MQ=255)

                                                                   tatcagGCCTCAGGTTCCTGATCCGGTACCCGGGGTACCACTACGCCTTCACGCGGCGCTTCAGGATTCGGCGCTGGCAGATTCATCAACTTCGCCAGaa                             <  2:3351420/100‑1 (MQ=255)

                                                                     tcagGCCTCAGGTTCCTGATCCGGTACCCGGGGTACCACTACGCCTTCACGCGGCGCTTCAGGATTCGGCGCTGGCAGATTCATCAACTTCGCCAGAATg                           >  1:715589/1‑100 (MQ=255)

                                                                      cagGCCTCAGGTTCCTGATCCGGTACCCGGGGTACCACTACGCCTTCACGCGGCGCTTCAGGATTCGGCGCTGGCAGATTCATCAACTTCGCCAGAATGc                          >  2:3514737/1‑100 (MQ=255)

                                                                      cagGCCTCAGGTTCCTGATCCGGTACCCGGGGTACCACTACGCCTTCACGCGGCGCTTCAGGATTCGGCGCTGGCAGATTCATCAACTTCGCCAGAATGc                          <  1:971052/100‑1 (MQ=255)

                                                                       agGCCTCAGGTTCCTGATCCGGTACCCGGGGTACCACTACGCCTTCACGCGGCGCTTCAGGATTCGGCGCTGGCAGATTCATCAACTTCGCCAGAATGCt                         >  2:1658582/1‑100 (MQ=255)

                                                                         gCCTCAGGTTCCTGATCCGGTACCCGGGGTACCACTACGCCTTCACGCGGCGCTTCAGGATTCGGCGCTGGCAGATTCATCAACTTCGCCAGAATGCTCg                       >  1:1097656/1‑100 (MQ=255)

                                                                         gCCTCAGGTTCCTGATCCGGTACCCGGGGTACCACTACGCCTTCACGCGGCGCTTCAGGATTCGGCGCTGGCAGATTCATCAACTTCGCCAGAATGCTCg                       >  1:1446896/1‑100 (MQ=255)

                                                                          ccTCAGGTTCCTGATCCGGTACCCGGGGTACCACTACGCCTTCACGCGGCGCTTCAGGATTCGGCGCTGGCAGATTCATCAACTTCGCCAGAATGCTCGc                      >  1:132972/1‑100 (MQ=255)

                                                                          ccTCAGGTTCCTGATCCGGTACCCGGGGTACCACTACGCCTTCACGCGGCGCTTCAGGATTCGGCGCTGGCAGATTCATCAACTTCGCCAGAATGCTCGc                      <  2:1209364/100‑1 (MQ=255)

                                                                           cTCAGGTTCCTGATCCGGTACCCGGGGTACCACTACGCCTTCACGCGGCGCTTCAGGATTCGGCGCTGGCAGATTCATCAACTTCGCCAGAATGCTCGcc                     <  1:1900369/100‑1 (MQ=255)

                                                                            tCAGGTTCCTGATCCGGTACCCGGGGTACCACTACGCCTTCACGCGGCGCTTCAGGATTCGGCGCTGGCAGATTCATCAACTTCGCCAGAATGCTCGCCa                    <  1:2263831/100‑1 (MQ=255)

                                                                              aGGTTCCTGATCCGGTACCCGGGGTACCACTACGCCTTCACGCGGCGCTTCAGGATTCGGCGCTGGCAGATTCATCAACTTCGCCAGAATGCTCGCCAGt                  >  2:589144/1‑100 (MQ=255)

                                                                              aGGTTCCTGATCCGGTACCCGGGGTACCACTACGCCTTCACGCGGCGCTTCAGGATTCGGCGCTGGCAGATTCATCAACTTCGCCAGAATGCTCGCCAGt                  >  2:3691100/1‑100 (MQ=255)

                                                                               ggTTCCTGATCCGGTACCCGGGGTACCACTACGCCTTCACGCGGCGCTTCAGGATTCGGCGCTGGCAGATTCATCAACTTCGCCAGAATGCTCGCCAGtt                 >  2:3823596/1‑100 (MQ=255)

                                                                                 ttCCTGATCCGGTACCCGGGGTACCACTACGCCTTCACGCGGCGCTTCAGGATTCGGCGCTGGCAGATTCATCAACTTCGCCAGAATGCTCGCCAGTTTc               >  1:3759230/1‑100 (MQ=255)

                                                                                 ttCCTGATCCGGTACCCGGGGTACCACTACGCCTTCACGCGGCGCTTCAGGATTCGGCGCTGGCAGATTCATCAACTTCGCCAGAATGCTCGCCAGTTTc               <  2:1026649/100‑1 (MQ=255)

                                                                                 ttCCTGATCCGGTACCCGGGGTACCACTACGCCTTCACGCGGCGCTTCAGGATTCGGCGCTGGCAGATTCATCAACTTCGCCAGAATGCTCGCCAGTTTc               <  2:2152451/100‑1 (MQ=255)

                                                                                   ccTGATCCGGTACCCGGGGTACCACTACGCCTTCACGCGGCGCTTCAGGATTCGGCGCTGGCAGATTCATCAACTTCGCCAGAATGCTCGCCAGTTTCAg             <  2:456304/100‑1 (MQ=255)

                                                                                    cTGATCCGGTACCCGGGGTACCACTACGCCTTCACGCGGCGCTTCAGGATTCGGCGCTGGCGGATTCATCAACTTCGCCAGAATGCTCGCCAGTTTCAgg            <  2:381995/100‑1 (MQ=255)

                                                                                    cTGATCCGGTACCCGGGGTACCACTACGCCTTCACGCGGCGCTTCAGGATTCGGCGCTGGCAGATTCATCAACTTCGCCAGAATGCTCGCCAGTTTCAgg            >  1:3253641/1‑100 (MQ=255)

                                                                                    cTGATCCGGTACCCGGGGTACCACTACGCCTTCACGCGGCGCTTCAGGATTCGGCGCTGGCAGATTCATCAACTTCGCCAGAATGCTCGCCAGTTTCAgg            <  2:715588/100‑1 (MQ=255)

                                                                                    cTGATCCGGTACCCGGGGTACCACTACGCCTTCACGAGGCGCTTCAGGATTCGGCGCTGGCAGATTCATCAACTTCGCCAGAATGCTCGCCAGTTTCAgg            >  1:2729103/1‑100 (MQ=255)

                                                                                    cTGATCCGGTACACGGGGTACCACTACGCCTTCACGCGGCGCTTCAGGATTCGGCGCTGGCAGATTCATCAACTTCGCCAGAATGCTCGCCAGTTTCAgn            <  2:1863597/100‑2 (MQ=255)

                                                                                     tGATCCGGTACCCGGGGTACCACTACGCCTTCACGCGGCGCTTCAGGATTCGGCGCTGGCAGATTCATCAACTTCGCCAGAATGCTCGCCAGTTTCAGgc           <  1:410963/100‑1 (MQ=255)

                                                                                     tGATCCGGTACCCGGGGTACCACTACGCCTTCACGCGGCGCTTCAGGATTCGGCGCTGGCAGATTCATCAACTTCGCCAGAATGCTCGCCAGTTTCAGgc           <  1:3293605/100‑1 (MQ=255)

                                                                                      gATCCGGTACCCGGGGTACCACTACGCCTTCACGCGGCGCTTCAGGATTCGGCGCTGGCAGATTCATCAACTTCGCCAGAATGCTCGCCAGTTTCAGgcg          >  2:2167482/1‑100 (MQ=255)

                                                                                        tCCGGTACCCGGGGTACCACTACGCCTTCACGCGGCGCTTCAGGATTCGGCGCTGGCAGATTCATCAACTTCGCCAGAATGCTCGCCAGTTTCAGGCGCa        <  2:3711089/100‑1 (MQ=255)

                                                                                          cGGTACCCGGGGTACCACTACGCCTTCACGCGGCGCTTCAGGATTCGGCGCTGGCAGATTCATCAACTTCGCCAGAATGCTCGCCAGTTTCAGGCGCAtt      >  1:3527522/1‑100 (MQ=255)

                                                                                           ggTACCCGGGGTACCACTACGCCTTCACGCGGCGCTTCAGGATTCGGCGCTGGCAGATTCATCAACTTCGCCAGAATGCTCGCCAGTTTCAGGCGCAttt     >  2:2089126/1‑100 (MQ=255)

                                                                                            gTACCCGGGGTACCACTACGCCTTCACGCGGCGCTTCAGGATTCGGCGCTGGCAGATTCATCAACTTCGCCAGAATGCTCGCCAGTTTCAGGCGCATTTc    <  1:3658675/100‑1 (MQ=255)

                                                                                            gTACCCGGGGTACCACTACGCCTTCACGCGGCGCTTCAGGATTCGGCGCTGGCAGATTCATCAACTTCGCCAGAATGCTCGCCAGTTTCAGGCGCATTTc    <  1:2487921/100‑1 (MQ=255)

                                                                                            gTACCCGGGGTACCACTACGCCTTCACGCGGCGCTTCAGGATTCGGCGCTGGCAGATTCATCAACTTCGCCAGAATGCTCGCCAGTTTCAGGCGCATTTc    <  2:2284040/100‑1 (MQ=255)

                                                                                              aCCCGGGGTACCACTACGCCTTCACGCGGCGCTTCAGGATTCGGCGCTGGCAGATTCATCAACTTCGCCAGAATGCTCGCCAGTTTCAGGCGCATTTCcg  >  2:899776/1‑100 (MQ=255)

GGTATCCGCTGATTCGTTACGGTGAGAATAGCAAAAGGGCAGAGCCAGTGGCCCTGCCCTTATCAGTTATCAGGCCTCAGGTTCCTGATCCGGTACCGGGGGTACCACTACGCCTTCACGCGGCGCTTCAGGATTCGGCGCTGGCAGATTCATCAACTTCGCCAGAATGCTCGCCAGTTTCAGGCGCATTTCCG  >  NC_000913/2432943‑2433136

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`