Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,946,035 G→T 16.1% intergenic (‑59/+80) ruvA ← / ← yobI component of RuvABC resolvasome, regulatory subunit/uncharacterized protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,946,0350GT16.1% 300.6 / 38.6 110intergenic (‑59/+80)ruvA/yobIcomponent of RuvABC resolvasome, regulatory subunit/uncharacterized protein
Reads supporting (aligned to +/- strand):  ref base G (59/33);  new base T (12/6);  total (71/39)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.88e-01

Only 100 of 116 total aligned reads displayed.

GGTTGTTTTTCAATGATGATGCCTCTGAGTCTGCCTATCACATGACGCTCCTGCGTAATGAATCAAAGATAATGCTGTATGATAAAAAAATGCTGGATAGATATCCAGCGAAGGATGAAGAAAACTTGCGAGGTGTCTCGATGAACTGAAAAATGGCACAGTATAATTTATTCTACAGGTTATATTGGAAGCAAA  >  NC_000913/1945936‑1946130
                                                                                                   |                                                                                               
ggTTGTTTTTCAATGATGATGCCTCTGAGTCTGCCTATCACATGACGCTCCTGCGTAATGAATCAAAGATAATGCTGTATGATAAAAAAATGCTGgatag                                                                                                 >  2:100797/1‑100 (MQ=255)
ggTTGTTTTTCAATGATGATGCCTCTGAGTCTGCCTATCACATGACGCTCCTGCGTAATGAATCAAAGATAATGCTGTATGATAAAAAAATGCTGgatag                                                                                                 <  2:2864119/100‑1 (MQ=255)
 gttgttTTTCAATGATGATGCCTCTGAGTCTGCCTATCACATGACGCTCCTGCGTAATGAATCAAAGATAATGCTGTATGATAAAAAAATGCTGgataga                                                                                                <  2:2549390/100‑1 (MQ=255)
  ttgttTTTCAATGATGATGCCTCTGAGTCTGCCTATCACATGACGCTCCTGCGTAATGAATCAAAGATAATGCTGTATGATAAAAAAATGCTGgatagat                                                                                               >  2:1603270/1‑100 (MQ=255)
    gttTTTCAATGATGATGCCTCTGAGTCTGCCTATCACATGACGCTCCTGCGTAATGAATCAAAGATAATGCTGTATGATAAAAAAATGCTGGATAGatat                                                                                             >  2:1500748/1‑100 (MQ=255)
      ttttCAATGATGATGCCTCTGAGTCTGCCTATCACATGACGCTCCTGCGTAATGAATCAAAGATAATGCTGTATGATAAAAAAATGCTGGATAGATATcc                                                                                           >  2:2368167/1‑100 (MQ=255)
      ttttCAATGATGATGCCTCTGAGTCTGCCTATCACATGACGCTCCTGCGTAATGAATCAAAGATAATGCTGTATGATAAAAAAATGCTGGATAGATATcc                                                                                           <  1:100797/100‑1 (MQ=255)
        ttCAATGATGATGCCTCTGCGTCTGCCTATCACATGACGCTCCTGCGTAATGAATCAAAGATAATGCTGTATGATAAAAAAATGCTGGATAGATATCCAg                                                                                         >  1:2535998/1‑100 (MQ=255)
         tCAATGATGATGCCTCTGAGTCTGCCTATCACATGACGCTCCTGCGTAATGAATCAAAGATAATGCTGTATGATAAAAAAATGCTGGATAGATATCCAGc                                                                                        <  1:2406847/100‑1 (MQ=255)
           aaTGATGATGCCTCTGAGTCTGCCTATCACATGACGCTCCTGCGTAATGAATCAAAGATAATGCTGTATGATAAAAAAATGCTGGATATCTATCCAGcat                                                                                      <  2:1745877/100‑3 (MQ=255)
           aaTGATGATGCCTCTGAGTCTGCCTATCACATGACGCTACTGCGTAATGAATCAAAGATAATGCTATAAGAAAAAAAAATGCTGGATAGATATCCAGCg                                                                                       <  1:2770165/99‑1 (MQ=255)
           aaTGAAGATGCCTCTGAGTCTGCCTATCACAAGACAATACTGCGTAATGAATCAAAGATAATAAAAAAAAAAAAAAAAATGCTGGATAGATATCCAGc                                                                                        <  1:202385/98‑1 (MQ=255)
             tgatgatgCCTCTGAGTCTGCTTATCACATGACGCTCCTGCGTAATGAATCAAAGATAATGCTGTATGATAAAAAAATGCTGGATAGATATCCAGCGAAg                                                                                    <  2:2625120/100‑1 (MQ=255)
             tgatgatgCCTCTGAGTCTGCCTATCACATGACGCTCCTGCGTAATGAATCAAAGATAATGCTGTATGATAAAAAAATGCTGGATAGATATCCAGCGAAg                                                                                    >  2:1309261/1‑100 (MQ=255)
             tgatgatgCCTCTGAGTCTGCCTATCACATGACGCTCCTGCGTAATGAATCAAAGATAATGCTGTATGATAAAAAAATGCTGGATAGATATCCAGCGAAg                                                                                    <  2:1850504/100‑1 (MQ=255)
              gatgatgCCTCTGAGTCTGCCTATCACATGACGCTCCTGCGTAATGAATCAAAGATAATGCTGTATGATAAAAAAATGCTGGATAGATATCCAGCGAAgg                                                                                   <  2:445128/100‑1 (MQ=255)
               atgatgCCTCTGAGTCTGCCTATCACATGACGCTCCTGCGTAATGAATCAAAGATAATGCTGTATGATAAAAAAATGCTGGATAGATATCCAGCGAAGGa                                                                                  >  1:759126/1‑100 (MQ=255)
                tgatgCCTCTGAGTCTGCCTATCACATGACGCTCCTGCGTAATGAATCAAAGATAATGCTGTATGATAAAAAAATGCTGGATATCTATCCAGcatttttt                                                                                 <  2:1567356/100‑8 (MQ=255)
                tgatgCCTCTGAGTCTGCCTATCACATGACGCTCCTGCGTAATGAATCAAAGATAATGCTGTATGATAAAAAAATGCTGGATATCTATCCAGcatttttt                                                                                 <  1:1458705/100‑8 (MQ=255)
                  atgCCTCTGAGTCTGCCTATCACATGACGCTCCTGCGTAATGAATCAAAGATAATGCTGTATGATAAAAAAATGCTGGATAGATATCCAGCGAAGGATga                                                                               >  2:2591144/1‑100 (MQ=255)
                  atgCCTCTGAGTCTGCCTATCACATGACGCTCCTGCGTAATGAATCAAAGATAATGCTGTATGATAAAAAAATGCTGGATAGATATCCAGCGAAGGATga                                                                               <  2:2007137/100‑1 (MQ=255)
                   tgCCTCTGAGTCTGCCTATCACATGACGCTCCTGCGTAATGAATCAAAGATAATGCTGTATGATAAAAAAATGCTGGATATCTATCCAGcatttttttat                                                                              <  2:1264340/100‑11 (MQ=38)
                   tgCCTCTGAGTCTGCCTATCACATGACGCTCCTGCGTAATGAATCAAAGATAATGCTGTATGATAAAAAAATGCTGGATAGATATCCAGCGAAGGATgaa                                                                              >  2:847812/1‑100 (MQ=255)
                    gCCTCTGAGTATGCCTAACACATTAATCTCCTGCGAAATGAATCAAAAATAATGCTGTATGATACAAAAATGCTGGATAGATATCCAGCGAAg                                                                                    <  1:1541397/93‑1 (MQ=255)
                         tGAGTCTGCCTATCACATGACGCTCCTGCGTAATGAATCAAAGATAATGCTGTATGATAAAAAAATGCTGGATAGATATCCAGCGAAGGATGAAGAAAAc                                                                        >  2:3166420/1‑100 (MQ=255)
                          gAGTCTGCCTATCACATGACGCTCCTGCGTAATGAATCAAAGATAATGCTGTATGATAAAAAAATGCTGGATAGATATCCAGCGGAGGATGAAGAAAACt                                                                       >  2:3644695/1‑100 (MQ=255)
                            gTCTGCCTATCACATGACGCTCCTGCGTAATGAATCAAAGATAATGCTGTATGATAAAAAAATGCTGGATAGATATCCAGCGAAGGATGAAGAAAACTTg                                                                     <  2:1464672/100‑1 (MQ=255)
                            gTCTGCCTATCACATGACGCTCCTGCGTAATGAATCAAAGATAATGCTGTATGATAAAAAAATGCTGGATAGATATCCAGCGAAGGATGAAGAAAACTTg                                                                     >  1:1272277/1‑100 (MQ=255)
                            gTCTGCCTATCACATGACGCTCCTGCGTAATGAATCAAAGATAATGCTGTATGATAAAAAAATGCTGGATAGATATCCAGCGAAGGATGAAGAAAACTTg                                                                     >  2:3122964/1‑100 (MQ=255)
                              cTGCCTATCACATGACGCTCCTGCGTAATGAATCAAAGATAATGCTGTATGATAAAAAAATGCTGGATAGATATCCAGCGAAGGATGAAGAAAACTTGCg                                                                   >  1:3024759/1‑100 (MQ=255)
                                gCCTATCACATGACGCTCCTGCGTAATGAATCAAAGATAATGCTGTATGATAAAAAAATGCTGGATAGATATCCAGCGAAGGATGAAGAAAACTTGCGAg                                                                 >  2:2797391/1‑100 (MQ=255)
                                   tATCACATGACGCTCCTGCGTAATGAATCAAAGATAATGCTGTATGATAAAAAAATGCTGGATAGATATCCAGCGAAGGATGAAGAAAACTTGCGAGgtg                                                              >  2:3052240/1‑100 (MQ=255)
                                   tATCACATGACGCTCCTGCGTAATGAATCAAAGATAATGCTGTATGATAAAAAAATGCTGGATAGATATCCAGCGAAGGATGAAGAAAAATTGAGAgg                                                                >  1:1888192/1‑98 (MQ=255)
                                      cgcaTGACGCTCCAGCGTAATGAATCAAAGAGAATGCTGTATGATAAAAAAATGCTGGATAGATATCCAGCGAAGGATGAAGAAAACTTGCGAGGTGtct                                                           >  2:2492760/3‑100 (MQ=255)
                                      cacaTGACGCTCCTGCGTAATGAATCAAAGATAATGCTGTATGATAAAAAAATGCTGGATAGATATCCAGCGAAGGATGAAGAAAACTTGCGAGGTGtct                                                           >  2:2901311/1‑100 (MQ=255)
                                      cacaTGACGCTCCTGCGTAATGAATCAAAGATAACGCTGTATGATAAAAAAATGCTGGATAGATATCCAGCGAAGGATGAAGAAAACTTGCGAGGTGtct                                                           >  1:2394418/1‑100 (MQ=255)
                                      caaaTGACGCACCTGCGTAATGAATCAAAGATAATGCAAAAAAAAAAAAAAATGCTGGATAGATATCCAGCGAAGGATGAAGAAAACTTGCGAGGTGtct                                                           <  1:3162063/97‑1 (MQ=255)
                                       acaTGACGCTCCTGCGTAATGAATCAAAGATAATGCTGTATGATAAAAAAATGCTGGATAGATATCCAGCGAAGGATGAAGAAAACTTGCGAGGTGtctc                                                          >  2:1979509/1‑100 (MQ=255)
                                        caTGACGCTCCTGCGTAATGAATCAAAGATAATGCTGTATGATAAAAAAATGCTGGATAGATATCCAGCGAAGGATGAAGAAAACTTGCGAGGTGTCTCg                                                         >  1:1151882/1‑100 (MQ=255)
                                        caTGACGCTCCTGCGTAATGAATCAAAGATAATGCTGTATGATAAAAAAATGCTGGATAGATATCCAGCGAAGGATGAAGAAAACTTGCGAGGTGTCTCg                                                         >  1:1469103/1‑100 (MQ=255)
                                          tGACGCTCCTGCGTAATGAATCAAAGATAATGCTGTATGATAAAAAAATGCTGGATAGATATCCAGCGAAGGATGAAGAAAACTTGCGAGGTGTCTCGAt                                                       >  2:28819/1‑100 (MQ=255)
                                              gCTCCTGCGTAATGAATCAAAGATAATGCTGTATGATAAAAAAATGCTGGATAGATATCCAGCGAAGGATGAAGAAAACTTGCGAGGTGTCTCGATGAAc                                                   <  2:3491398/100‑1 (MQ=255)
                                               cTCCTGCGTAATGAATCAAAGATAATGCTGTATGATATAAAAATGCTGGATAGATATCCAGCGAAGGATGAAGAAAACTTGCGAGGTGTCTCGATGAACt                                                  >  1:1984094/1‑100 (MQ=255)
                                               cTCCTGCGTAATGAATCAAAGATAATGCTGTATGATAAAAAAATGCTGGCTAGATATCCAGCGAAGGATGAAGAAAACTTGCGAGGTGTCTCGATGAACt                                                  <  2:460587/100‑1 (MQ=255)
                                               cTCCTGCGTAATGAATCAAAGATAATGCTGTATGATAAAAAAATGCTGGATAGATATCCAGCGAAGGATGAAGAAAACTTGCGAGGTGTCTCGATGAACt                                                  <  1:1067079/100‑1 (MQ=255)
                                                  cTGCGTAATGAATCAAAGATAATGCTGTATGATAAAAAAATGCTGGATAGATATCCAGCGAAGGATGAAGAAAACTTGCGAGGTGTCTCGATGAACt                                                  >  1:2876657/1‑97 (MQ=255)
                                                  cTGCGTAATGAATCAAAGATAATGCTGTATGATAAAAAAATGCTGGATAGATATCCAGCGAAGGATGAAGAAAACTTGCGAGGTGTCTCGATGAACTGa                                                <  1:8429/99‑1 (MQ=255)
                                                   tGCGTAATGAATTAAAGATAATGCGGTATGATAAAAAAATGCTGGATAGATATCCAGCGAAGGAGGAAGAAAACTTGCGAGGTGTCTCGATGAACTGaaa                                              <  1:239418/100‑1 (MQ=255)
                                                   tGCGTAATGAATCAAAGATAATGCTGTATGATAAAAAAATGCTGGATAGATATCCAGCGAAGGATGAAGAAAACTTGCGAGGTGTCTCGATGAACTGaaa                                              <  2:1984107/100‑1 (MQ=255)
                                                    gCGTTATGAATCAAAGATAATGCTGTATGATAAAAAAATGCTGGATAGATATCCAGCGAAGGATGAAGAAAACTTGCGAGGTGTCTCGATGAACTGaaaa                                             >  1:325905/1‑100 (MQ=255)
                                                    gCGTAATGAGTCAAGGATAATGATAGAAGAAAAAAAGATGCTGGATAGATATCCAGCGAAAGATGAAAAAAACATGCGAGGTGTCTAGATTAAcag                                                 >  1:3306155/1‑94 (MQ=255)
                                                     cGTAATGAATCAAAGATAATGCTGTATGATAAAAAAATACTGGATAGATATCCAGCGAAGGATGAAGAAAACTTACGAGGTGTCTCGATGAACTGaaaaa                                            >  1:1621623/1‑100 (MQ=255)
                                                      gTAATGAATCAAAGATAATGCTGTATGATAAAAAAATGCTGGATAGATATCCAGCGAAGGATGAAGAAAACTTGCGAGGTGTCTCGATGAACTGAAAAAt                                           <  2:958119/100‑1 (MQ=255)
                                                       tAATGAATCAAAGATAATGATGTACGAGAAAAAAATGCTGGAGAGATATCCAGCGAAGGATGAAGAAAATTTGGTATTTGTCTCGAGGGACTGAAAAATg                                          >  1:1227738/1‑100 (MQ=255)
                                                        aaTGAATCAAAGATAATGCTGTATGATAAAAAAATGCTGGATAGATATCCAGCGAAGGATGAAGAAAACTTGCGAGGTGTCTCGATGAACTGAAAAATgg                                         >  1:2898404/1‑100 (MQ=255)
                                                        aaTGAATCAAAGATAATGCTGAATGATAAAAAAATGCTGGATAGATATCCAGCGAAGGATGAAGAAAACTTGCGAGGTGTCTCGATGAACTGAAAAATgg                                         <  1:2438873/100‑1 (MQ=255)
                                                          tGAATCAAAGATAATGCTGTATGATAAAAAAATGCTGGATAGATATCCAGCGAAGGATGAAGAAAACTTGCGAGGTGTCTCGATGAACTGAAAAATGGca                                       >  1:2009383/1‑100 (MQ=255)
                                                           gAATCAAAGATAATGCTGTATGATAAAAAAATGCTGGATAGATATCCAGCGAAGGATGAAGAAAACTTGCGAGGTGTCTCGATGAACTGAAAAATGGcac                                      <  2:3537067/100‑1 (MQ=255)
                                                            aaTCAAAGATAATGCTGTATGATAAAAAAATGCTGGATAGATATCCAGCGAAGGATGAAGAAAACTTGCGAGGTGTCTCGATGAACTGAAAAATGGcaca                                     >  1:452742/1‑100 (MQ=255)
                                                             aTCAAAGATAATGCTGTATGATAAAAAAATGCTGGATAGATATCCAGCGAAGGATGAAGAAAACTTGCGAGGTGTCTCGATGAACTGAAAAATGGCACAg                                    >  2:2936080/1‑100 (MQ=255)
                                                             aTCAAAGATAATGCTGTATGATAAAAAAATGCTGGATAGATATCCAGCGAAGGATGAAGAAAACTTGCGAGGTGTCTCGATGAACTGAAAAATGGCACAg                                    >  2:842926/1‑100 (MQ=255)
                                                                aaaGATAATGCTGTATGATAAAAAAATGCTGGATAGATATCCAGCGAAGGATGAAGAAAACTTGCGAGGTGTCTCGATGAACTGAAAAATGGCACAGtat                                 >  2:925834/1‑100 (MQ=255)
                                                                aaaGATAATGCTGTATGATAAAAAAATGCTGGATAGATATCCAGCGAAGGATGAAGAAAACTTGCGAGGTGTCTCGATGAACTGAAAAATGGCACAGtat                                 >  1:324118/1‑100 (MQ=255)
                                                                 aaGATAATGCTGTATGATAAAAAAATGCTGGATAGATATCCAGCGAAGGATGAAGAAAACTTGCGAGGTGTCTCGATGAACTGAAAAATGGCACAGtata                                >  1:922341/1‑100 (MQ=255)
                                                                  aGATAATGCTGTATGATAAAAAAATGCTGGATAGATATCCAGCGAAGGATGAAGAAAACTTGCGAGGTGTCTCGATGAACTGAAAAATGGCACAGTATaa                               >  2:488069/1‑100 (MQ=255)
                                                                  aGATAATGCTGTATGATAAAAAAATGCTGGATAGATATCCAGCGAAGGATGAAGAAAACTTGCGAGGTGTCTCGATGAACTGAAAAATGGCACAGTATaa                               >  2:3284948/1‑100 (MQ=255)
                                                                     tAATGCTGAAAAAAAAAAAAATGCTGGATAGATATCCAGCGAAGGATGAAGAAAACTTGCGAGGTGTCTCGATGAACTGAAAAATGGCACAGTATAAttt                            <  1:2071578/100‑1 (MQ=255)
                                                                      aaTGCAGTAAGAAAAAAAAATGCTGGAAAGATATCCAACAAAGGATGAAGAAAACTTGCGAGGTGTAAAAAAAAAAAAAAAAATGGCACAGTATAAttt                            <  1:579302/99‑1 (MQ=255)
                                                                         gCTGTATGATAAAAAAATGCTGGATAGATATCCAGCGAAGGATGAAGAAAACTTGCGAGGTGTCTCGATGAACTGAAAAATGGCACAGTATAATTTATTc                        >  2:1902434/1‑100 (MQ=255)
                                                                          cTGTATGATAAAAAAATGCTGGATAGATATCCAGCGAAGGATGAAGAAAACTTGCGAGGTGTCTCGATGAACTGAAAAATGGCACAGTATAATTTATTCt                       >  2:758293/1‑100 (MQ=255)
                                                                          cTGTATGATAAAAAAATGCTGGATAGATATCCAGCGAAGGATGAAGAAAACTTGCGAGGTGTCTCGATGAACTGAAAAATGGCACAGTATAATTTATTCt                       <  2:569560/100‑1 (MQ=255)
                                                                           tGTATGATAAAAAAATGCTGAATAGATATCCAGCGAAGGATGAAGAAAACTTGCGAGGTGTCTCGATGAACTGAAAAATGGAACAGTATAATTTATTCt                       >  1:547057/1‑99 (MQ=255)
                                                                            gTATGATAAAAAAATGCTGGATAGATATCCAGCGAAGGATGAAGAAAACTTGCGAGGTGTCTCGATGAACTGAAAAATGGCACAGTATAATTTATTCTAc                     >  2:642054/1‑100 (MQ=255)
                                                                            gTATGATAAAAAAATGCTGGATAGATATCCAGCGAAGGATGAAGAAAACTTGCGAGGTGTCTCGATGAACTGAAAAATGGCACAGTATAATTTATTCTAc                     >  2:933760/1‑100 (MQ=255)
                                                                             tATGATAAAAAAATGCTGGATAGATATCCAGCGAAGGATGAAGAAAACTTGCGAGGTGTCTCGATGAACTGAAAAATGGCACAGTATAATTTATTCTACa                    <  2:387535/100‑1 (MQ=255)
                                                                              aTGATAAAAAAATGCTGGATAGATATCCAGCGAAGGATGAAGAAAACTTGCGAGGTGTCTCGATGAACTGAAAAATGGCACAGTATAATTTATTCTACAg                   <  2:3155082/100‑1 (MQ=255)
                                                                              aTGATAAAAAAATGCTGGATAGATATCCAGCGAAGGATGAAGAAAACTTGCGAGGTGTCTCGATGAACTGAAAAATGGCACAGTATAATTTATTCTACAg                   <  2:1780231/100‑1 (MQ=255)
                                                                              aTGATAAAAAAATGCTGGATAGATATCCAGCGAAGGATGAAGAAAACTTGCGAGGTGTCTCGATGAACTGAAAAATGGCACAGTATAATTTATTCTACAg                   <  2:111901/100‑1 (MQ=255)
                                                                              aTGATAAAAAAATGCTGGATAGATATCCAGCGAAGGATGAAGAAAACTTGCGAGGTGTATAAAAAAAAAAAAAAATGGCACAGTATAATTTATTCTACAg                   <  1:1206439/100‑1 (MQ=255)
                                                                                 atatAAAAATTTTTGATATATATTCAGTTAATGATGAATAAAACTTGCGAGGTGTCTCGATTAACTTAAAAATGGCACAGTATAATTTATTCTACAGGt                 <  1:2912626/99‑1 (MQ=255)
                                                                                 aTAAAAAAACGCTGGATAGATATCCAGCGAAGGATGAAGAAAACTTGCGAGGTGTCTCGATGACCTGAAAAATGGCACAGTATAATCTATTCTAGAGGtt                >  1:1046410/1‑100 (MQ=255)
                                                                                  tAAAAAAATGCTGGATAGATATCCAGCGAAGGATGAAGAAAACTTGCGAGGTGTCTCGATGAACTGAAAAATGGCACAGTATAATTTATTCTACAGGTta               <  2:3142272/100‑1 (MQ=255)
                                                                                  tAAAAAAATGCTGGATAGATATCCAGCGAAGGATGAAGAAAACTTGCGAGGTGTCTCGATGAACTGAAAAATGGCACAGTATAATTTATTCTACAGGTta               >  2:3385364/1‑100 (MQ=255)
                                                                                  tAAAAAAATGCTGGATAGATATCCAGCGAAGGATGAAGAAAACTTGCGAGGTGTCTCGATGAACTGAAAAATGGCACAGTATAATTTATTCTACAGGTta               >  2:2015598/1‑100 (MQ=255)
                                                                                  tAAAAAAATGCTGGATAGATATACAGCGAAGGATGAAGAAAACTTGCGAGGTGTCTCGATGAAGTGAAAAATGGCACAGTATAATTTAtta                        >  2:2942779/1‑90 (MQ=255)
                                                                                   aaaaaaaTGCTGGATAGATATCCAGCGAAGGATGAAGAAAACTTGCGAGGTGTCTCGATGAACTGAAAAATGGCACAGTATAATTTATTCTACAGGTtat              <  1:868950/100‑1 (MQ=255)
                                                                                      ccttcgCTGGATATCTATCCAGCGAAGGATGAAGAAAACTTGCGAGGTGTCTCGATGAACTGAAAAATGGCACAGTATAATTTATTCTACAGGTTATAtt           >  1:2764886/6‑100 (MQ=255)
                                                                                      aaaaTGCTGGATAGATATCCAGCGAAGGATGAAGAAAACTTGCGAGGTGTCTCGATGAACTGAAAAATGGCACAGTATAATTTATTCTACAGGTTATAtt           >  2:419471/1‑100 (MQ=255)
                                                                                       cttcgCTGGATATCTATCCAGCGAAGGATGAAGAAAACTTGCGAGGTGTCTCGATGAACTGAAAAATGGCACAGTATAATTTATTCTACAGGTTATATTg          >  2:2881029/5‑100 (MQ=255)
                                                                                       cttcgCTGGATATCTATCCAGCGAAGGATGAAGAAAACTTGCGAGGTGTCTCGATGAACTGAAAAATGGCACAGTATAATTTATTCTACAGGTTATATTg          >  1:3349573/5‑100 (MQ=255)
                                                                                       aaaTGCTGGATAGATATCCAGCGAAGGATGAAGAAAACTTGCGAGGTGTCTCGATGAACTGAAAAATGGCACAGTATAATTTATTCTACAGGTTATATTg          >  2:2626471/1‑100 (MQ=255)
                                                                                         aTGCTGGATAGATATCCAGCGAAGGATGAAGAAAACTTGCGAGGTGTCTCGATGAACTGAAAAATGGCACAGTATAATTTATTCTACAGGTTATATTGGa        <  1:2182917/100‑1 (MQ=255)
                                                                                          cgCTGGATATCTATCCAGCGAAGGATGAAGAAAACTTGCGAGGTGTCTCGATGAACTGAAAAATGGCACAGTATAATTTATTCTACAGGTTATATTGGaa       >  1:777402/2‑100 (MQ=255)
                                                                                          cgCTGGATATCTATCCAGCGAAGGATGAAGAAAACTTGCGAGGTGTCTCGATGAACTGAAAAATGGCACAGTATAATTTATTCTACAGGTTATATTGGa        >  1:185578/2‑99 (MQ=255)
                                                                                           gCTGGATATCTATCCAGCGAAGGATGAAGATAACTTGCGAGGTGTCTCGATGAACTGAAAAATGGCACAGTATAATTTATTCTACAGGTTATATTgtcag      >  1:1406041/1‑96 (MQ=255)
                                                                                           gCTGGATATCTATCCAGCGAAGGATGAAGAAAACTTGCGAGGTGTCTCGATGAACTGAAAAATGGCACAGTATAATTTATTCTACAGGTTATATTGTAAg      >  1:3287258/1‑100 (MQ=255)
                                                                                           gCTGGATATCTATCCAGCGAAGGATGAAGAAAACTTGCGAGGTGTCTCGATGAACTGAAAAATGGCACAGTATAATTTATTCTACAGGTTATATTGGAAg      >  1:1563746/1‑100 (MQ=255)
                                                                                           gCTGGATATCTATCCAGCGAAGGATGAAGAAAACTTGCGAGGTGTCTCGATGAACTGAAAAATGGCACAGTATAATTTATTCTACAGGTTATATTGGAAg      >  1:1727960/1‑100 (MQ=255)
                                                                                            cTGGATAGATATCCCGCGAAGGATGAAGAAAACTTGCGAGGTGTCTCGATGAACTGAAAAATGGCACAGTATCATTTATTCTACAGGTTATATTGGAAGc     >  1:801204/1‑100 (MQ=255)
                                                                                               gatagataTCCAGCGAAGGATGAAGAAAACTTGCGAGGTGTCTCGATGAACTGAAAAATGGCACAGTATAATTTATTCTACAGGTTATATTGGAAGCaaa  >  2:2954266/1‑100 (MQ=255)
                                                                                                   |                                                                                               
GGTTGTTTTTCAATGATGATGCCTCTGAGTCTGCCTATCACATGACGCTCCTGCGTAATGAATCAAAGATAATGCTGTATGATAAAAAAATGCTGGATAGATATCCAGCGAAGGATGAAGAAAACTTGCGAGGTGTCTCGATGAACTGAAAAATGGCACAGTATAATTTATTCTACAGGTTATATTGGAAGCAAA  >  NC_000913/1945936‑1946130

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: