Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,946,036 A→C 15.6% intergenic (‑60/+79) ruvA ← / ← yobI component of RuvABC resolvasome, regulatory subunit/uncharacterized protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,946,0360AC15.6% 278.8 / 43.7 109intergenic (‑60/+79)ruvA/yobIcomponent of RuvABC resolvasome, regulatory subunit/uncharacterized protein
Reads supporting (aligned to +/- strand):  ref base A (58/34);  new base C (12/5);  total (70/39)
Fisher's exact test for biased strand distribution p-value = 7.84e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.47e-01

Only 100 of 114 total aligned reads displayed.

GTTGTTTTTCAATGATGATGCCTCTGAGTCTGCCTATCACATGACGCTCCTGCGTAATGAATCAAAGATAATGCTGTATGATAAAAAAATGCTGGATAGATATCCAGCGAAGGATGAAGAAAACTTGCGAGGTGTCTCGATGAACTGAAAAATGGCACAGTATAATTTATTCTACAGGTTATATTGGAAGCAAA  >  NC_000913/1945937‑1946130
                                                                                                   |                                                                                              
gttgttTTTCAATGATGATGCCTCTGAGTCTGCCTATCACATGACGCTCCTGCGTAATGAATCAAAGATAATGCTGTATGATAAAAAAATGCTGgataga                                                                                                <  2:2549390/100‑1 (MQ=255)
 ttgttTTTCAATGATGATGCCTCTGAGTCTGCCTATCACATGACGCTCCTGCGTAATGAATCAAAGATAATGCTGTATGATAAAAAAATGCTGgatagat                                                                                               >  2:1603270/1‑100 (MQ=255)
   gttTTTCAATGATGATGCCTCTGAGTCTGCCTATCACATGACGCTCCTGCGTAATGAATCAAAGATAATGCTGTATGATAAAAAAATGCTGGATAGatat                                                                                             >  2:1500748/1‑100 (MQ=255)
     ttttCAATGATGATGCCTCTGAGTCTGCCTATCACATGACGCTCCTGCGTAATGAATCAAAGATAATGCTGTATGATAAAAAAATGCTGGATAGATATcc                                                                                           <  1:100797/100‑1 (MQ=255)
     ttttCAATGATGATGCCTCTGAGTCTGCCTATCACATGACGCTCCTGCGTAATGAATCAAAGATAATGCTGTATGATAAAAAAATGCTGGATAGATATcc                                                                                           >  2:2368167/1‑100 (MQ=255)
       ttCAATGATGATGCCTCTGCGTCTGCCTATCACATGACGCTCCTGCGTAATGAATCAAAGATAATGCTGTATGATAAAAAAATGCTGGATAGATATCCAg                                                                                         >  1:2535998/1‑100 (MQ=255)
       ttCAATGATGATGCCTCTGAGTCTGCCTATCACATGACGCTCCTGCGTAATGAATCAAAGATAATGCTGTATGATAAAAAAATGCTGGATAGATATCCAg                                                                                         >  1:2028740/1‑100 (MQ=255)
       ttCAATGATGATGCCTCTGAGTCTGCCTATCACATGACGCTCCTGCGTAATGAATCAAAGATAATGCTGTATGATAAAAAAATGCTGGATAGATATCCAg                                                                                         <  2:1814846/100‑1 (MQ=255)
        tCAATGATGATGCCTCTGAGTCTGCCTATCACATGACGCTCCTGCGTAATGAATCAAAGATAATGCTGTATGATAAAAAAATGCTGGATAGATATCCAGc                                                                                        <  1:2406847/100‑1 (MQ=255)
          aaTGATGATGCCTCTGAGTCTGCCTATCACATGACGCTCCTGCGTAATGAATCAAAGATAATGCTGTATGATAAAAAAATGCTGGATATCTATCCAGcat                                                                                      <  2:1745877/100‑3 (MQ=255)
          aaTGATGATGCCTCTGAGTCTGCCTATCACATGACGCTCCTGCGTAATGAATCAAAGATAATGCTGTATGATAAAAAAATGCTGGATAGATATCCAGCGa                                                                                      >  2:2349288/1‑100 (MQ=255)
          aaTGATGATGCCTCTGAGTCTGCCTATCACATGACGCTACTGCGTAATGAATCAAAGATAATGCTATAAGAAAAAAAAATGCTGGATAGATATCCAGCg                                                                                       <  1:2770165/99‑1 (MQ=255)
          aaTGAAGATGCCTCTGAGTCTGCCTATCACAAGACAATACTGCGTAATGAATCAAAGATAATAAAAAAAAAAAAAAAAATGCTGGATAGATATCCAGc                                                                                        <  1:202385/98‑1 (MQ=255)
            tgatgatgCCTCTGAGTCTGCTTATCACATGACGCTCCTGCGTAATGAATCAAAGATAATGCTGTATGATAAAAAAATGCTGGATAGATATCCAGCGAAg                                                                                    <  2:2625120/100‑1 (MQ=255)
            tgatgatgCCTCTGAGTCTGCCTATCACATGACGCTCCTGCGTAATGAATCAAAGATAATGCTGTATGATAAAAAAATGCTGGATATCTATCCAGcatt                                                                                     <  2:395988/99‑4 (MQ=255)
            tgatgatgCCTCTGAGTCTGCCTATCACATGACGCTCCTGCGTAATGAATCAAAGATAATGCTGTATGATAAAAAAATGCTGGATAGATATCCAGCGAAg                                                                                    >  2:1309261/1‑100 (MQ=255)
             gatgatgCCTCTGAGTCTGCCTATCACATGACGCTCCTGCGTAATGAATCAAAGATAATGCTGTATGATAAAAAAATGCTGGATAGATATCCAGCGAAgg                                                                                   <  2:445128/100‑1 (MQ=255)
              atgatgCCTCTGAGTCTGCCTATCACATGACGCTCCTGCGTAATGAATCAAAGATAATGCTGTATGATAAAAAAATGCTGGATAGATATCCAGCGAAGGa                                                                                  >  1:759126/1‑100 (MQ=255)
               tgatgCCTCTGAGTCTGCCTATCACATGACGCTCCTGCGTAATGAATCAAAGATAATGCTGTATGATAAAAAAATGCTGGATATCTATCCAGcatttttt                                                                                 <  2:1567356/100‑8 (MQ=255)
               tgatgCCTCTGAGTCTGCCTATCACATGACGCTCCTGCGTAATGAATCAAAGATAATGCTGTATGATAAAAAAATGCTGGATATCTATCCAGcatttttt                                                                                 <  1:1458705/100‑8 (MQ=255)
                 atgCCTCTGAGTCTGCCTATCACATGACGCTCCTGCGTAATGAATCAAAGATAATGCTGTATGATAAAAAAATGCTGGATAGATATCCAGCGAAGGATga                                                                               <  2:2007137/100‑1 (MQ=255)
                 atgCCTCTGAGTCTGCCTATCACATGACGCTCCTGCGTAATGAATCAAAGATAATGCTGTATGATAAAAAAATGCTGGATAGATATCCAGCGAAGGATga                                                                               >  2:2591144/1‑100 (MQ=255)
                  tgCCTCTGAGTCTGCCTATCACATGACGCTCCTGCGTAATGAATCAAAGATAATGCTGTATGATAAAAAAATGCTGGATATCTATCCAGcatttttttat                                                                              <  2:1264340/100‑11 (MQ=38)
                  tgCCTCTGAGTCTGCCTATCACATGACGCTCCTGCGTAATGAATCAAAGATAATGCTGTATGATAAAAAAATGCTGGATAGATATCCAGCGAAGGATgaa                                                                              >  2:847812/1‑100 (MQ=255)
                   gCCTCTGAGTATGCCTAACACATTAATCTCCTGCGAAATGAATCAAAAATAATGCTGTATGATACAAAAATGCTGGATAGATATCCAGCGAAg                                                                                    <  1:1541397/93‑1 (MQ=255)
                    ccTCTGAGTCTGCCTATCACATGACGCTCCTGCGTAATGAATCAAAGATAATGCTGTATGATAAAAAAATGCTGGATAGATATCCAGCGAAGGATgaaga                                                                            >  2:2939031/1‑100 (MQ=255)
                       ctGAGTCTGCCTATCACATGACGCTCCTGCGTAATGAATCAAAGATAATGCTGTATGATAAAAAAATGCTGGATAGATATCCAGCGAAGGATGAAGaaaa                                                                         >  1:3117668/1‑100 (MQ=255)
                        tGAGTCTGCCTATCACATGACGCTCCTGCGTAATGAATCAAAGATAATGCTGTATGATAAAAAAATGCTGGATAGATATCCAGCGAAGGATGAAGAAAAc                                                                        >  2:3166420/1‑100 (MQ=255)
                           gTCTGCCTATCACATGACGCTCCTGCGTAATGAATCAAAGATAATGCTGTATGATAAAAAAATGCTGGATAGATATCCAGCGAAGGATGAAGAAAACTTg                                                                     >  1:1272277/1‑100 (MQ=255)
                           gTCTGCCTATCACATGACGCTCCTGCGTAATGAATCAAAGATAATGCTGTATGATAAAAAAATGCTGGATAGATATCCAGCGAAGGATGAAGAAAACTTg                                                                     <  2:1464672/100‑1 (MQ=255)
                           gTCTGCCTATCACATGACGCTCCTGCGTAATGAATCAAAGATAATGCTGTATGATAAAAAAATGCTGGATAGATATCCAGCGAAGGATGAAGAAAACTTg                                                                     >  2:3122964/1‑100 (MQ=255)
                             cTGCCTATCACATGACGCTCCTGCGTAATGAATCAAAGATAATGCTGTATGATAAAAAAATGCTGGATAGATATCCAGCGAAGGATGAAGAAAACTTGCg                                                                   >  1:3024759/1‑100 (MQ=255)
                               gCCTATCACATGACGCTCCTGCGTAATGAATCAAAGATAATGCTGTATGATAAAAAAATGCTGGATAGATATCCAGCGAAGGATGAAGAAAACTTGCGAg                                                                 >  2:2797391/1‑100 (MQ=255)
                                  tATCACATGACGCTCCTGCGTAATGAATCAAAGATAATGCTGTATGATAAAAAAATGCTGGATAGATATCCAGCGAAGGATGAAGAAAACTTGCGAGgtg                                                              >  2:3052240/1‑100 (MQ=255)
                                  tATCACATGACGCTCCTGCGTAATGAATCAAAGATAATGCTGTATGATAAAAAAATGCTGGATAGATATCCAGCGAAGGATGAAGAAAAATTGAGAgg                                                                >  1:1888192/1‑98 (MQ=255)
                                     cacaTGACGCTCCTGCGTAATGAATCAAAGATAATGCTGTATGATAAAAAAATGCTGGATAGATATCCAGCGAAGGATGAAGAAAACTTGCGAGGTGtct                                                           >  2:2901311/1‑100 (MQ=255)
                                     cacaTGACGCTCCTGCGTAATGAATCAAAGATAACGCTGTATGATAAAAAAATGCTGGATAGATATCCAGCGAAGGATGAAGAAAACTTGCGAGGTGtct                                                           >  1:2394418/1‑100 (MQ=255)
                                     caaaTGACGCACCTGCGTAATGAATCAAAGATAATGCAAAAAAAAAAAAAAATGCTGGATAGATATCCAGCGAAGGATGAAGAAAACTTGCGAGGTGtct                                                           <  1:3162063/97‑1 (MQ=255)
                                      acaTGACGCTCCTGCGTAATGAATCAAAGATAATGCTGTATGATAAAAAAATGCTGGATAGATATCCAGCGAAGGATGAAGAAAACTTGCGAGGTGtctc                                                          >  2:1979509/1‑100 (MQ=255)
                                       caTGACGCTCCTGCGTAATGAATCAAAGATAATGCTGTATGATAAAAAAATGCTGGATAGATATCCAGCGAAGGATGAAGAAAACTTGCGAGGTGTCTCg                                                         >  1:1151882/1‑100 (MQ=255)
                                       caTGACGCTCCTGCGTAATGAATCAAAGATAATGCTGTATGATAAAAAAATGCTGGATAGATATCCAGCGAAGGATGAAGAAAACTTGCGAGGTGTCTCg                                                         >  1:1469103/1‑100 (MQ=255)
                                             gCTCCTGCGTAATGAATCAAAGATAATGCTGTATGATAAAAAAATGCTGGATAGATATCCAGCGAAGGATGAAGAAAACTTGCGAGGTGTCTCGATGAAc                                                   <  2:3491398/100‑1 (MQ=255)
                                              cTCCTGCGTAATGAATCAAAGATAATGCTGTATGATATAAAAATGCTGGATAGATATCCAGCGAAGGATGAAGAAAACTTGCGAGGTGTCTCGATGAACt                                                  >  1:1984094/1‑100 (MQ=255)
                                              cTCCTGCGTAATGAATCAAAGATAATGCTGTATGATAAAAAAATGCTGGCTAGATATCCAGCGAAGGATGAAGAAAACTTGCGAGGTGTCTCGATGAACt                                                  <  2:460587/100‑1 (MQ=255)
                                              cTCCTGCGTAATGAATCAAAGATAATGCTGTATGATAAAAAAATGCTGGATAGATATCCAGCGAAGGATGAAGAAAACTTGCGAGGTGTCTCGATGAACt                                                  <  1:1067079/100‑1 (MQ=255)
                                                 cTGCGTAATGAATCAAAGATAATGCTGTATGATAAAAAAATGCTGGATAGATATCCAGCGAAGGATGAAGAAAACTTGCGAGGTGTCTCGATGAACTGa                                                <  1:8429/99‑1 (MQ=255)
                                                  tGCGTAATGAATCAAAGATAATGCTGTATGATAAAAAAATGCTGGATAGATATCCAGCGAAGGATGAAGAAAACTTGCGAGGTGTCTCGATGAACTGaaa                                              <  2:1984107/100‑1 (MQ=255)
                                                   gCGTTATGAATCAAAGATAATGCTGTATGATAAAAAAATGCTGGATAGATATCCAGCGAAGGATGAAGAAAACTTGCGAGGTGTCTCGATGAACTGaaaa                                             >  1:325905/1‑100 (MQ=255)
                                                   gCGTAATGAGTCAAGGATAATGATAGAAGAAAAAAAGATGCTGGATAGATATCCAGCGAAAGATGAAAAAAACATGCGAGGTGTCTAGATTAAcag                                                 >  1:3306155/1‑94 (MQ=255)
                                                    cGTAATGAATCAAAGATAATGCTGTATGATAAAAAAATACTGGATAGATATCCAGCGAAGGATGAAGAAAACTTACGAGGTGTCTCGATGAACTGaaaaa                                            >  1:1621623/1‑100 (MQ=255)
                                                     gTAATGAATCAAAGATAATGCTGTATGATAAAAAAATGCTGGATAGATATCCAGCGAAGGATGAAGAAAACTTGCGAGGTGTCTCGATGAACTGAAAAAt                                           >  2:684200/1‑100 (MQ=255)
                                                     gTAATGAATCAAAGATAATGCTGTATGATAAAAAAATGCTGGATAGATATCCAGCGAAGGATGAAGAAAACTTGCGAGGTGTCTCGATGAACTGAAAAAt                                           <  2:958119/100‑1 (MQ=255)
                                                      tAATGAATCAAAGATAATGATGTACGAGAAAAAAATGCTGGAGAGATATCCAGCGAAGGATGAAGAAAATTTGGTATTTGTCTCGAGGGACTGAAAAATg                                          >  1:1227738/1‑100 (MQ=255)
                                                       aaTGAATCAAAGATAATGCTGTATGATAAAAAAATGCTGGATAGATATCCAGCGAAGGATGAAGAAAACTTGCGAGGTGTCTCGATGAACTGAAAAATgg                                         >  1:2898404/1‑100 (MQ=255)
                                                       aaTGAATCAAAGATAATGCTGTATGATAAAAAAATGCTGGATAGATATCCAGCGAAGGATGAAGAAAACTTGCGAGGTGTCTCGATGAACTGAAAAATg                                          >  1:2454469/1‑99 (MQ=255)
                                                       aaTGAATCAAAGATAATGCTGAATGATAAAAAAATGCTGGATAGATATCCAGCGAAGGATGAAGAAAACTTGCGAGGTGTCTCGATGAACTGAAAAATgg                                         <  1:2438873/100‑1 (MQ=255)
                                                         tGAATCAAAGATAATGCTGTATGATAAAAAAATGCTGGATAGATATCCAGCGAAGGATGAAGAAAACTTGCGAGGTGTCTCGATGAACTGAAAAATGGca                                       >  1:2009383/1‑100 (MQ=255)
                                                          gAATCAAAGATAATGCTGTATGATAAAAAAATGCTGGATAGATATCCAGCGAAGGATGAAGAAAACTTGCGAGGTGTCTCGATGAACTGAAAAATGGcac                                      <  2:3537067/100‑1 (MQ=255)
                                                           aaTCAAAGATAATGCTGTATGATAAAAAAATGCTGGATAGATATCCAGCGAAGGATGAAGAAAACTTGCGAGGTGTCTCGATGAACTGAAAAATGGcaca                                     >  1:452742/1‑100 (MQ=255)
                                                            aTCAAAGATAATGCTGTATGATAAAAAAATGCTGGATAGATATCCAGCGAAGGATGAAGAAAACTTGCGAGGTGTCTCGATGAACTGAAAAATGGCACAg                                    >  2:2936080/1‑100 (MQ=255)
                                                            aTCAAAGATAATGCTGTATGATAAAAAAATGCTGGATAGATATCCAGCGAAGGATGAAGAAAACTTGCGAGGTGTCTCGATGAACTGAAAAATGGCACAg                                    >  2:842926/1‑100 (MQ=255)
                                                               aaaGATAATGCTGTATGATAAAAAAATGCTGGATAGATATCCAGCGAAGGATGAAGAAAACTTGCGAGGTGTCTCGATGAACTGAAAAATGGCACAGtat                                 >  2:925834/1‑100 (MQ=255)
                                                               aaaGATAATGCTGTATGATAAAAAAATGCTGGATAGATATCCAGCGAAGGATGAAGAAAACTTGCGAGGTGTCTCGATGAACTGAAAAATGGCACAGtat                                 >  1:324118/1‑100 (MQ=255)
                                                                aaGATAATGCTGTATGATAAAAAAATGCTGGATAGATATCCAGCGAAGGATGAAGAAAACTTGCGAGGTGTCTCGATGAACTGAAAAATGGCACAGtata                                >  1:922341/1‑100 (MQ=255)
                                                                 aGATAATGCTGTATGATAAAAAAATGCTGGATAGATATCCAGCGAAGGATGAAGAAAACTTGCGAGGTGTCTCGATGAACTGAAAAATGGCACAGTATaa                               >  2:3284948/1‑100 (MQ=255)
                                                                 aGATAATGCTGTATGATAAAAAAATGCTGGATAGATATCCAGCGAAGGATGAAGAAAACTTGCGAGGTGTCTCGATGAACTGAAAAATGGCACAGTATaa                               >  2:488069/1‑100 (MQ=255)
                                                                    tAATGCTGAAAAAAAAAAAAATGCTGGATAGATATCCAGCGAAGGATGAAGAAAACTTGCGAGGTGTCTCGATGAACTGAAAAATGGCACAGTATAAttt                            <  1:2071578/100‑1 (MQ=255)
                                                                     aaTGCTGTATGATAAAAAAATGCTGGATAGATATCCAGCGAAGGATGAAGAAAACTTGCGAGGTGTCTCGATGAACTGAAAAATGGCACAGTATAATTTa                           >  1:1288570/1‑100 (MQ=255)
                                                                     aaTGCAGTAAGAAAAAAAAATGCTGGAAAGATATCCAACAAAGGATGAAGAAAACTTGCGAGGTGTAAAAAAAAAAAAAAAAATGGCACAGTATAAttt                            <  1:579302/99‑1 (MQ=255)
                                                                        gCTGTATGATAAAAAAATGCTGGATAGATATCCAGCGAAGGATGAAGAAAACTTGCGAGGTGTCTCGATGAACTGAAAAATGGCACAGTATAATTTATTc                        >  2:1902434/1‑100 (MQ=255)
                                                                         cTGTATGATAAAAAAATGCTGGATAGATATCCAGCGAAGGATGAAGAAAACTTGCGAGGTGTCTCGATGAACTGAAAAATGGCACAGTATAATTTATTCt                       <  2:569560/100‑1 (MQ=255)
                                                                         cTGTATGATAAAAAAATGCTGGATAGATATCCAGCGAAGGATGAAGAAAACTTGCGAGGTGTCTCGATGAACTGAAAAATGGCACAGTATAATTTATTCt                       >  2:758293/1‑100 (MQ=255)
                                                                          tGTATGATAAAAAAATGCTGAATAGATATCCAGCGAAGGATGAAGAAAACTTGCGAGGTGTCTCGATGAACTGAAAAATGGAACAGTATAATTTATTCt                       >  1:547057/1‑99 (MQ=255)
                                                                           gTATGATAAAAAAATGCTGGATAGATATCCAGCGAAGGATGAAGAAAACTTGCGAGGTGTCTCGATGAACTGAAAAATGGCACAGTATAATTTATTCTAc                     >  2:933760/1‑100 (MQ=255)
                                                                            tATGATAAAAAAATGCTGGATAGATATCCAGCGAAGGATGAAGAAAACTTGCGAGGTGTCTCGATGAACTGAAAAATGGCACAGTATAATTTATTCTACa                    <  2:387535/100‑1 (MQ=255)
                                                                             aTGATAAAAAAATGCTGGATAGATATCCAGCGAAGGATGAAGAAAACTTGCGAGGTGTCTCGATGAACTGAAAAATGGCACAGTATAATTTATTCTACAg                   <  2:1780231/100‑1 (MQ=255)
                                                                             aTGATAAAAAAATGCTGGATAGATATCCAGCGAAGGATGAAGAAAACTTGCGAGGTGTCTCGATGAACTGAAAAATGGCACAGTATAATTTATTCTACAg                   <  2:3155082/100‑1 (MQ=255)
                                                                             aTGATAAAAAAATGCTGGATAGATATCCAGCGAAGGATGAAGAAAACTTGCGAGGTGTCTCGATGAACTGAAAAATGGCACAGTATAATTTATTCTACAg                   <  2:111901/100‑1 (MQ=255)
                                                                             aTGATAAAAAAATGCTGGATAGATATCCAGCGAAGGATGAAGAAAACTTGCGAGGTGTATAAAAAAAAAAAAAAATGGCACAGTATAATTTATTCTACAg                   <  1:1206439/100‑1 (MQ=255)
                                                                                atatAAAAATTTTTGATATATATTCAGTTAATGATGAATAAAACTTGCGAGGTGTCTCGATTAACTTAAAAATGGCACAGTATAATTTATTCTACAGGt                 <  1:2912626/99‑1 (MQ=255)
                                                                                aTAAAAAAATGCTGGATAGATATCCAGCGAAGGATGAAGAAAACTTGCGAGGTGTCTCGATGAACTGAAAAATGGCACAGTATAATTTATTCTACAGGtt                >  1:1672309/1‑100 (MQ=255)
                                                                                aTAAAAAAACGCTGGATAGATATCCAGCGAAGGATGAAGAAAACTTGCGAGGTGTCTCGATGACCTGAAAAATGGCACAGTATAATCTATTCTAGAGGtt                >  1:1046410/1‑100 (MQ=255)
                                                                                 tcatccttcgCTGGATATCTATCCAGCGAAGGATGAAGAAAACTTGCGAGGTGTCTCGATGAACTGAAAAATGGCACAGTATAATTTATTCTACAGGTta               >  2:2762969/10‑100 (MQ=38)
                                                                                 tAAAAAAATGCTGGATAGATATCCAGCGAAGGATGAAGAAAACTTGCGAGGTGTCTCGATGAACTGAAAAATGGCACAGTATAATTTATTCTACAGGTta               >  2:3385364/1‑100 (MQ=255)
                                                                                 tAAAAAAATGCTGGATAGATATCCAGCGAAGGATGAAGAAAACTTGCGAGGTGTCTCGATGAACTGAAAAATGGCACAGTATAATTTATTCTACAGGTta               >  2:2015598/1‑100 (MQ=255)
                                                                                 tAAAAAAATGCTGGATAGATATACAGCGAAGGATGAAGAAAACTTGCGAGGTGTCTCGATGAAGTGAAAAATGGCACAGTATAATTTAtta                        >  2:2942779/1‑90 (MQ=255)
                                                                                  aaaaaaaTGCTGGATAGATATCCAGCGAAGGATGAAGAAAACTTGCGAGGTGTCTCGATGAACTGAAAAATGGCACAGTATAATTTATTCTACAGGTtat              <  1:868950/100‑1 (MQ=255)
                                                                                     ccttcgCTGGATATCTATCCAGCGAAGGATGAAGAAAACTTGCGAGGTGTCTCGATGAACTGAAAAATGGCACAGTATAATTTATTCTACAGGTTATAtt           >  1:2764886/6‑100 (MQ=255)
                                                                                     aaaaTGCTGGATAGATATCCAGCGAAGGATGAAGAAAACTTGCGAGGTGTCTCGATGAACTGAAAAATGGCACAGTATAATTTATTCTACAGGTTATAtt           >  2:419471/1‑100 (MQ=255)
                                                                                      cttcgCTGGATATCTATCCAGCGAAGGATGAAGAAAACTTGCGAGGTGTCTCGATGAACTGAAAAATGGCACAGTATAATTTATTCTACAGGTTATATTg          >  2:2881029/5‑100 (MQ=255)
                                                                                      cttcgCTGGATATCTATCCAGCGAAGGATGAAGAAAACTTGCGAGGTGTCTCGATGAACTGAAAAATGGCACAGTATAATTTATTCTACAGGTTATATTg          >  1:3349573/5‑100 (MQ=255)
                                                                                      cttcgCTGGATATCTATCCAGCGAAGGATGAAGAAAACTTGCGAGGTGTCTCGATGAACTGAAAAATGGCACAGTATAATTTATTCTACAGGTTATATTg          >  1:3352523/5‑100 (MQ=255)
                                                                                      aaaTGCTGGATAGATATCCAGCGAAGGATGAAGAAAACTTGCGAGGTGTCTCGATGAACTGAAAAATGGCACAGTATAATTTATTCTACAGGTTATATTg          >  2:2626471/1‑100 (MQ=255)
                                                                                        aTGCTGGATAGATATCCAGCGAAGGATGAAGAAAACTTGCGAGGTGTCTCGATGAACTGAAAAATGGCACAGTATAATTTATTCTACAGGTTATATTGGa        <  1:2182917/100‑1 (MQ=255)
                                                                                          gCTGGATATCTATCCAGCGAAGGATGAAGAAAACTTGCGAGGTGTCTCGATGAACTGAAAAATGGCACAGTATAATTTATTCTACAGGTTATATTGGAAg      >  1:1563746/1‑100 (MQ=255)
                                                                                          gCTGGATATCTATCCAGCGAAGGATGAAGAAAACTTGCGAGGTGTCTCGATGAACTGAAAAATGGCACAGTATAATTTATTCTACAGGTTATATTGGAAg      >  1:1727960/1‑100 (MQ=255)
                                                                                          gCTGGATATCTATCCAGCGAAGGATGAAGAAAACTTGCGAGGTGTCTCGATGAACTGAAAAATGGCACAGTATAATTTATTCTACAGGTTATATTGGAAg      >  1:81618/1‑100 (MQ=255)
                                                                                           cTGGATAGATATCCCGCGAAGGATGAAGAAAACTTGCGAGGTGTCTCGATGAACTGAAAAATGGCACAGTATCATTTATTCTACAGGTTATATTGGAAGc     >  1:801204/1‑100 (MQ=255)
                                                                                              gatagataTCCAGCGAAGGATGAAGCAAACTTGCGAGGTGTCTCGATGAACTGAAAAATGGCACAGTATAATTTATTCTACAGGTTATATTGGAAGCaaa  <  2:3220511/100‑1 (MQ=255)
                                                                                              gatagataTCCAGCGAAGGATGAAGAAAACTTGCGAGGTGTCTCGATGAACTGAAAAATGGCACAGTATAATTTATTCTACAGGTTATATTGGAAGCaaa  >  2:2954266/1‑100 (MQ=255)
                                                                                                   |                                                                                              
GTTGTTTTTCAATGATGATGCCTCTGAGTCTGCCTATCACATGACGCTCCTGCGTAATGAATCAAAGATAATGCTGTATGATAAAAAAATGCTGGATAGATATCCAGCGAAGGATGAAGAAAACTTGCGAGGTGTCTCGATGAACTGAAAAATGGCACAGTATAATTTATTCTACAGGTTATATTGGAAGCAAA  >  NC_000913/1945937‑1946130

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: