Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,081,058 C→T 15.1% V69M (GTG→ATG) ‡ sbmC ← DNA gyrase inhibitor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,081,0580CT15.1% 327.8 / 45.1 133V69M (GTG→ATG) sbmCDNA gyrase inhibitor
Reads supporting (aligned to +/- strand):  ref base C (52/61);  new base T (8/12);  total (60/73)
Fisher's exact test for biased strand distribution p-value = 8.08e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.01e-01

Only 100 of 149 total aligned reads displayed.

CTACACGAGCTACCGCCACCGCATACTGACCACCTGTAATTTCTGTCAGAATGACGCCCTCACTGTTTTCGGGAAGCGTAAAGTAACCCGGCACCGTCACGACGGTGTCGCAGCGTAATTTTTCGGCGGGTGTTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCA  >  NC_000913/2080959‑2081157
                                                                                                   |                                                                                                   
cTACACGAGCTACCGCCACCGCATACTGACCACCTGTAATTTCTGTCAGAATGACGCCCTCACTGTTTTCGGGAAGCGTAAAGTAACCCGGCACCGTCac                                                                                                     >  1:811844/1‑100 (MQ=255)
 tACACGAGCTACCGCCACCGCATACTGACCACCTGTAATTTCTGTCAGAATGACGCCCTCACTGTTTTCGGGAAGCGTAAAGTAACCCGGCACCGTCacg                                                                                                    >  1:552954/1‑100 (MQ=255)
  acacGAGCTACCGCCACCGCATACTGACCACCTGTAATTTCTGTCAGAATGACGCCCTCACTGTTTTCGGGAAGCGTAAAGTAACCCGGCACCGTCacga                                                                                                   >  1:1344907/1‑100 (MQ=255)
   cacGAGCTACCGCCACCGCATACTGACCACCTGTAATTTCTGTCAGAATGACGCCCTCACTGTTTTCGGGAAGCGTAAAGTAACCCGGCACCGTCacgac                                                                                                  <  1:1282126/100‑1 (MQ=255)
   cacGAGCTACCGCCACCGCATACTGACCACCTGTAATTTCTGTCAGAATGACGCCCTCACTGTTTTCGGGAAGCGTAAAGTAACCCGGCACCGTCacgac                                                                                                  >  2:3265994/1‑100 (MQ=255)
   cacGAGCTACCGCCACCGCATACTGACCACCTGTAATTTCTGTCAGAATGACGCCCTCACTGTTTTCGGGAAGCGTAAAGTAACCCGGCACCGTCacgac                                                                                                  >  2:2354627/1‑100 (MQ=255)
     cGAGCTACCGCCACCGCATACTGACCACCTGTAATTTCTGTCAGAATGACGCCCTCACTGTTTTCGGGAAGCGTAAAGTAACCCGGCACCGTCacgac                                                                                                  <  1:1314800/98‑1 (MQ=255)
        gCTACCGCCACCGCATACTGACCACCTGTAATTTCTGTCAGAATGACGCCCTCACTGTTTTCGGGAAGCGTAAAGTAACCCGGCACCGTCACGACGgtgt                                                                                             <  1:749652/100‑1 (MQ=255)
         cTACCGCCACCGCATACTGACCACCTGTAATTTCTGTCAGAATGACGCCCTCACTGTTTTCGGGAAGCGTAAAGTAACCCGGCACCGTCGTGACGgtgcc                                                                                            <  2:2553575/100‑3 (MQ=255)
          tACCGCCACCGCATACTGACCACCTGTAATTTCTGTCAGAATGACGCCCTCACTGTTTTCGGGAAGCGTAAAGTAACCCGGCACCGTCACGACGGTGTCg                                                                                           >  1:2688159/1‑100 (MQ=255)
          tACCGCCACCGCATAATGACCACCTGTAATTTCTGTCAGAATGACGCCCTCACTGTTTTCGGGAAGCGTAAAGTAACCCGGCACCGTCACGACGGTGTCg                                                                                           <  2:3652022/100‑1 (MQ=255)
           aCCGCCACCGCATACTGACCACCTGTAATTTCTGTCAGAATGACGCCCTCACTGTTTTCGGGAAGCGTAAAGTAACCCGGCACCGTCGTGACGGTGCCgg                                                                                          <  2:1891851/100‑2 (MQ=255)
           aCCGCCACCGCATACTGACCACCTGTAATTTCTGTCAGAATGACGCCCTCACTGTTTTCGGGAAGCGTAAAGTAACCCGGCACCGTCACGACGgtgt                                                                                             >  1:3273544/1‑97 (MQ=255)
           aCCGCCACCGCATACTGACCACCTGTAATTTCTGTCAGAATGACGCCCTCACTGTTTTCGGGAAGCGTAAAGTAACCCGGCACCGTCACGACGGTGTCGc                                                                                          <  1:3221978/100‑1 (MQ=255)
           aCCGCCACCGCATACTGACCACCTGTAATTTCTGTCAGAATGACGCCCTCACTGTTTTCGGGAAGCGTAAAGTAACCCGACACCGTCGTGACGGTGCCgg                                                                                          <  1:1537193/100‑2 (MQ=255)
           aCCGCCACCGCATACTGACCACCTGTAATTTCTGTCAGAATGACGCCCTCACTGTTTTCGGGAAGCGTAAAGTAACCCGACACCGTCGTGACGGTGCCgg                                                                                          <  1:1077577/100‑2 (MQ=255)
            ccGCCACCGCATACTGACCACCTGTAATTTCTGTCAGAATGACGCCCTCACTGTTTTCGGGAAGCGTAAAGTAACCCGGCACCGTCACGACGGTGTCGCa                                                                                         >  2:1492163/1‑100 (MQ=255)
            ccGCCACCGCATACTGACCACCTGTAATTTCTGTCAGAATGACGCCCTCACTGTTTTCGGGAAGCGTAAAGTAACCCGGCACCGTCACGACGGTGTCGCa                                                                                         >  1:3228477/1‑100 (MQ=255)
            ccGCCACCGCATACTGACCACCTGTAATTTCTGTCAGAATGACGCCCTCACTGTTTTCGGGAAGCGTAAAGTAACCCGACACCGTCGTGACGGTGCCggg                                                                                         <  2:2172241/100‑3 (MQ=255)
            ccGCCACCGCATACTGACCACCTGTAATTTCTGTCAGAATGACGCCCTCACTGTTTTCGGGAAGCGTAAAGTAACCCGACACCGTCGTGACGGTGCCggg                                                                                         <  1:410525/100‑3 (MQ=255)
             cGCCACCGCATACTGACCACCTGTAATTTCTGTCAGAATGACGCCCTCACTGTTTTCGGGAAGCGTAAAGTAACCCGGCACCGTCACGACGGTGTCGCAg                                                                                        >  2:3418598/1‑100 (MQ=255)
              gCCACCGCATACTGACCACCTGTAATTTCTGTCAGAATGACGCCCTCACTGTTTTCGGGAAGCGTAAAGTAACCCGGCACCGTCACGACGGTGTCGCAGc                                                                                       >  2:1441949/1‑100 (MQ=255)
               ccACCGCATACTGACCACCTGTAATTTCTGTCAGAATGACGCCCTCACTGTTTTCGGGAAGCGTAAAGTAACCCGGCACCGTCACGACGGTGTCGCAGCg                                                                                      >  2:932093/1‑100 (MQ=255)
               ccACCGCATACTGACCACCTGTAATTTCTGTCAGAATGACGCCCTCACTGTTTTCGGGAAGCGTAAAGTAACCCGGCACCGTCACGACGGTGTCGCAGCg                                                                                      >  2:932105/1‑100 (MQ=255)
                cACCGCATACTGACCACCTGTAATTTCTGTCAGAATGACGCCCTCACTGTTTTCGGGAAGCGTAAAGTAACCCGACACCGTCGTGACGgtgccgggttac                                                                                     <  2:3073591/100‑10 (MQ=37)
                 aCCGCATACTGACCACCTGTAATTTCTGTCAGAATGACGCCCTCACTGTTTTCGGGAAGCGTAAAGTAACCCGGCACCGTCACGACGGTGTCGCAGCGTa                                                                                    >  2:2278630/1‑100 (MQ=255)
                  ccGCATACTGACCACCTGTAATTTCTGTCAGAATGACGCCCTCACTGTTTTCGGGAAGCGTAAAGTAACCCGGCACCGTCACGACGGTGTCGCAGCGTaa                                                                                   >  1:686739/1‑100 (MQ=255)
                  ccGCATACTGACCACCTGTAATTTCTGTCAGAATGACGCCCTCACTGTTTTCGGGAAGCGTAAAGTAACCCGGCACCGTCACGACGGTGTCGCAGCGTaa                                                                                   >  2:2972966/1‑100 (MQ=255)
                   cGCATACTGACCACCTGTAATTTCTGTCAGAATGACGCGCTGACTGTTCTCGGGAAGCGTAAAGTAACCCGGCACCGTCACGACGGTGACGCAGCGTaa                                                                                   >  1:3589918/1‑99 (MQ=255)
                   cGCATACTGACCACCTGTAATTTCTGTCAGAATGACGCCCTCACTGTTTTCGGGAAGCGTAAAGTAACCCGGCACCGTCACGACGGTGTCGCAGCGTAAt                                                                                  <  2:908707/100‑1 (MQ=255)
                    gCATACTGACCACCTGTAATTTCTGTCAGAATGACGCCCTCACTGTTTTCGGGAAGCGTAAAGTAACCCGGCACCGTCACGACGGTGTCGCAGCGTAAtt                                                                                 <  2:1117475/100‑1 (MQ=255)
                    gCATACTGACCACCTGTAATTTCTGTCAGAATGACGCCCTCACTGTTTTCGGGAAGCGTAAAGTAACCCGGCACCGTCACGACGGTGTCGCAGCGTAAtt                                                                                 <  1:3380822/100‑1 (MQ=255)
                    gCATACTGACCACCTGTAATTTCTGTCAGAATGACGCCCTCACTGTTTTCGGGAAGCGTAAAGTAAACCGGCACCGTCACGACGGCGTCGCAGCGTAAt                                                                                  >  1:3548496/1‑99 (MQ=255)
                     cATACTGACCACCTGTAATTTCTGTCAGAATGACGCCCTCACTGTTTTCGGGAAGCGTAAAGTAACCCGGCACCGTCACGACGGTGTCGCAGCGTAAttt                                                                                <  2:827312/100‑1 (MQ=255)
                       tACTGACCACCTGTAATTTCTGTCAGAATGACGCCCTCACTGTTTTCGGGAAGCGTAAAGTAACCCGGCACCGTCACGACGGTGTCGCAGCGTAAttttt                                                                              <  2:3234696/100‑1 (MQ=255)
                         cTGACCACCTGTAATTTCTGTCAGAATGACGCCCTCACTGTTTTCGGGAAGCGTAAAGTAACCCGGCACCGTCACGACGGTGTCGCAGCGTAATTTTTcn                                                                            <  2:271898/100‑2 (MQ=255)
                         cTGACCACCTGTAATTTCTGTCAGAATGACGCCCTCACTGTTTTCGGGAAGCGTAAAGTAACCCGGCACCGTCACGACGGTGTCGCAGCGTAATTTTTcg                                                                            >  2:1436950/1‑100 (MQ=255)
                             ccaccTGTAATTTCTGTCAGAATGACGCCCTCACTGTTTTCGGGAAGCGTAAAGTAACCCGGCACCGTCACGACGGTGTCGCAGCGTAATTTTTcggcgg                                                                        <  2:3550010/100‑1 (MQ=255)
                              caccTGTAATTTCTGTCAGAATGACGCCCTCACTGTTTTCGGGAAGCGTAAAGTAACCCGGCACCGTCACGACGGTGTCGCAGCGTAATTTTTCGGCggg                                                                       >  1:1925152/1‑100 (MQ=255)
                                ccTGTAATTTCTGTCAGAATGACGCCCTCACTGTTTTCGGGAAGCGTAAAGTAACCCGGCACCGTCACGACGGTGTCGCAGCGTAATTTTTCGGCGGgtg                                                                     <  2:1664983/100‑1 (MQ=255)
                                ccTGTAATTTCTGTCAGAATGACGCCCTCACTGTTTTCGGGAAGCGTAAAGTAACCCGGCACCGTCACGACGGTGTCGCAGCGTAATTTTTCGGCGGgtg                                                                     >  2:3381381/1‑100 (MQ=255)
                                 cTGTAATTTCTGTCAGAATGACGCCCTCACTGTTTTCGGGAAGCGTAAAGTAACCCGGCACCGTCACGACGGTGTCGCAGCGTAATTTTTCGGCGGgtgt                                                                    >  1:2468755/1‑100 (MQ=255)
                                   gTAATTTCTGTCAGAATGACGCCCTCACTGTTTTCGGGAAGCGTAAAGTAACCCGGCACCGTCACGACGGTGTCGCAGCGTAATTTTTCGGCGGGTGttt                                                                  <  2:57398/100‑1 (MQ=255)
                                     aaTTTCTGTCAGAATGACGCCCTCACTGTTTTCGGGAAGCGTAAAGTAACCCGGCACCGTCACGACGGTGTCGCAGCGTAATTTTTCGGCGGGTGTTTCa                                                                <  1:2104972/100‑1 (MQ=255)
                                       tttCTGTCAGAATGACGCCCTCACTGTTTTCGGGAAGCGTAAAGTAACCCGGCACCGTCACGACGGTGTCGCAGCGTAATTTTTCGGCGGGTGTTTCATc                                                              >  2:419855/1‑100 (MQ=255)
                                        ttCTGTCAGAATGACGCCCTCACTGTTTTCGGGAAGCGTAAAGTAACCCGGCACCGTCACGACGGTGTCGCAGCGTAATTTTTCGGCGGGTGTTTCATCt                                                             <  2:3445390/100‑1 (MQ=255)
                                            gTCAGAATGACGCCCTCACTGTTTTCGGGAAGCGTAAAGTAACCCGGCACCGTCACGACGGTGTCGCAGCGTAATTTTTCGGCGGGTGTTTCATCTGGAt                                                         <  1:932087/100‑1 (MQ=255)
                                            gTCAGAATGACGCCCTCACTGTTTTCGGGAAGCGTAAAGTAACCCGGCACCGTCACGACGGTGTCGCAGCGTAATTTTTCGGCGGGTGTTTCATCTGGAt                                                         >  2:1803106/1‑100 (MQ=255)
                                            gTCAGAATGACGCCCTCACTGTTTTCGGGAAGCGTAAAGTAACCCGGCACCGTCACGACGGTGTCGCAGCGTAATTTTTCGGCGGGTGTTTCATCTGGAt                                                         <  1:2063960/100‑1 (MQ=255)
                                            gTCAGAATGACGCCCTCACTGTTTTCGGGAAGCGTAAAGTAACCCGGCACCGTCACGACGGTGTCGCAGCGTAATTTTTCGGCGGGTGTTTCATCTGGAt                                                         >  1:572080/1‑100 (MQ=255)
                                              cAGAATGACGCCCTCACTGTTTTCGGGAAGCGTAAAGTAACCCGGCACCGTCACGACGGTGTCGCAGCGTAATTTTTCGGCGGGTGTTTCATCTGGATTg                                                       >  2:1605548/1‑100 (MQ=255)
                                                 aaTGACTCCCTCACTGTTTTCGGGAAGCGTAAAGTAACCCGGCACCGTCACGACGGTGTCTCAGCGTAATTTTTCGGCGGGTGTTTCATCTGAATTGTCg                                                    <  1:2475866/100‑1 (MQ=255)
                                                 aaTGACGCCCTCACTGTTTTCGGGAAGCGTAAAGTAACCCGGCACCGTCACGACGGTGTCGCAGCGTAATTTTTCGGCGGGTGTTTCATCTGGATTGTCg                                                    <  1:2895614/100‑1 (MQ=255)
                                                 aaTGACGCCCTCACTGTTTTCGGGAAGCGTAAAGTAACCCGGCACCGTCACGACGGTGTCGCAGCGTAATTTTTCGGCGGGTGTTTCATCTGGATTGTCg                                                    <  1:2972945/100‑1 (MQ=255)
                                                 aaTGACGCCCTCACTGTTTTCGGGAAGCGTAAAGTAACCCGGCACCGTCACGACGGTGTAGCAGCGTAATTTTTCGGCGGGTGTTTCATCTGGATTGTcn                                                    <  1:439315/100‑2 (MQ=255)
                                                  aTGACGCCCTCACTGTTTTCGGGAAGCGTAAAGTAACCCGGCACCGTCACGACGGTGTCGCAGCGTAATTTTTCGGCGGGTGTTTCATCTGGATTGTCGt                                                   >  2:1102218/1‑100 (MQ=255)
                                                   tGACGCCCTCACTGTTTTCGGGAAGCGTAAAGTAACCCGGCACCGTCACGACGGTGTCGCAGCGTAATTTTTCGGCGGGTGTTTCATCTGGATTGTc                                                     <  1:3307024/97‑1 (MQ=255)
                                                    gACGCCCTCACTGTTTTCGGGAAGCGTAAAGTAACCCGGCACCGTCACGACGGTGTCGCAGCGTAATTTTTCGGCGGGTGTTTCATCTGGATTGTCGTaa                                                 <  1:145604/100‑1 (MQ=255)
                                                      cGCCCTCACTGTTTTCGGGAAGCGTAAAGTAACCCGGCACCGTCACGACGGTGTCGCAGCGTAATTTTTCGGCGGGTGTTTCATCTGGATTGTCGTAATa                                               <  2:3008170/100‑1 (MQ=255)
                                                       gCCCTCACTGTTTTCGGGAAGCGTAAAGTAACCCGGCACCGTCACGACGGTGTCGCAGCGTAATTTTTCGGCGGGTGTTTCATCTGGATTGTCGTAATAg                                              <  2:2456747/100‑1 (MQ=255)
                                                            cACTGTTTTCGGGAAGCGTAAAGTAACCCGGCACCGTCACGACGGTGTCGCAGCGTAATTTTTCGGCGGGTGTTTCATCTGGATTGTCGTAATAGACAGc                                         <  2:145970/100‑1 (MQ=255)
                                                             aCTGTTTTCGGGAAGCGTAAAGTAACCCGGCACCGTCACGACGGTGTCGCAGCGTAATTTTTCGGCGGGTGTTTCATCTGGATTGTCGTAATAGACAGCa                                        <  1:221571/100‑1 (MQ=255)
                                                              cTGTTTTCGGGAAGCGTAAAGTAACCCGGCACCGTCACGACGGTGTCGCAGCGTAATTTTTCGGCGGGTGTTTCATCTGGATTGTCGTAATAGACAGCaa                                       >  1:2215322/1‑100 (MQ=255)
                                                              cTGTTTTCGGGAAGCGTAAAGTAACCCGGCACCGTCACGACGGTGTCGCAGCGTAATTTTTCGGCGGGTGTTTCATCTGGATTGTCGTAATAGACAGCaa                                       >  1:267086/1‑100 (MQ=255)
                                                               tGTTTTCGGGAAGCGTAAAGTAACCCGGCACCGTCACGACGGTGTCGCAGCGTAATTTTTCGGCGGGTGTTTCATCTGGATTGTCGTAATAGACAGCa                                        >  1:770495/1‑98 (MQ=255)
                                                                gTTTTCGGGAAGCGTAAAGTAACCCGGCACCGTCACGACGGTGTCGCAGCGTAATTTTTCGGCGGGTGTTTCATCTGGATTGTCGTAATAGACAGCAAcc                                     <  2:443593/100‑1 (MQ=255)
                                                                    tCGGGAAGCGTAAAGTAACCCGGCACCGTCACGACGGTGTCGCAGCGTAATTTTTCGGCGGGTGTTTCATCTGGATTGTCGTAATAGACAGCAACCCACt                                 >  2:3005969/1‑100 (MQ=255)
                                                                      gggAAGCGTAAAGTAACCCGGCACCGTCACGACGGTGTCGCAGCGTAATTTTTCGGCGGGTGTTTCATCTGGATTGTCGTAATAGACAGCAACCCACTcc                               >  2:3082460/1‑100 (MQ=255)
                                                                         aaGCGTAAAGTAACCCGGCACCGTCACGACGGTGTCGCAGCGTAATTTTTCGGCGGGTGTTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTc                            <  1:1289711/100‑1 (MQ=255)
                                                                           gCGTAAAGTAACCCGGCACCGTCACGACGGTGTCGCAGCGTAATTTTTCGGCGGGTGTTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTcag                          <  2:2500613/100‑3 (MQ=255)
                                                                           gCGTAAAGTAACCCGGCACCGTCACGACGGTGTCGCAGCGTAATTTTTCGGCGGGTGTTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCgg                          <  2:2982613/100‑1 (MQ=255)
                                                                            cGTAAAGTAACCCGGCACCGTCACGACGGTGTCGCAGCGTAATTTTTCGGCGGGTGTTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGc                         >  2:1823021/1‑100 (MQ=255)
                                                                             gTAAAGTAACCCGGCACCGTCACGACGGTGTCGCAGCGTAATTTTTCGGCGGGTGTTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGca                        >  1:401709/1‑100 (MQ=255)
                                                                             gTAAAGTAACCCGGCACCGTCACGACGGTGTCGCAGCGTAATTTTTCGGCGGGTGTTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGca                        >  2:63013/1‑100 (MQ=255)
                                                                             gTAAAGTAACCCGGCACCGTCACGACGGTGTCGCAGCGTAATTTTTCGGCGGGTGTTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGca                        >  2:422264/1‑100 (MQ=255)
                                                                             gTAAAGTAACCCGGCACCGTCACGACGGTGTCGCAGCGTAATTTTTCGGCGGGTGTTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGca                        >  1:682339/1‑100 (MQ=255)
                                                                                aaGTAACCCGGCACCGTCACGACGGTGTCGCAGCGTAATTTTTCGGCGGGTGTTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACaa                     >  1:2046783/1‑100 (MQ=255)
                                                                                     ctgcgacACCGTCGTGACGGTGTCGCAGCGTAATTTTTCGGCGGGTGTTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAatat                  >  2:2747543/7‑98 (MQ=255)
                                                                                     ctgcgacACCGTCGTGACGGTGTCGCAGCGTAATTTTTCGGCGGGTGTTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATAttt                >  2:1211851/7‑100 (MQ=255)
                                                                                     ctgcgacACCGTCGTGACGGTGTCGCAGCGTAATTTTTCGGCGGGTGTTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATAttt                >  2:356715/7‑100 (MQ=255)
                                                                                     ctgcgacACCGTCGTGACGGTGTCGCAGCGTAATTTTTCGGCGGGTGTTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATAttt                >  2:2086907/7‑100 (MQ=255)
                                                                                       gcgacACCGTCGTGACGGTGTCGCAGCGTAATTTTTCGGCGGGTGTTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATAttttt              >  2:2056002/5‑100 (MQ=255)
                                                                                       gcgacACCGTCGTGACGGTGTCGCAGCGTAATTTTTCGGCGGGTGTTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATAttttt              >  2:1889549/5‑100 (MQ=255)
                                                                                        cGGCACCGTCACGACGGTGTCGCAGCGTAATTTTTCGGCGGGTGTTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTg             <  1:599289/100‑1 (MQ=255)
                                                                                        cGGCACCGTCACGACGGTGTCGCAGCGTAATTTTTCGGCGGGTGTTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTg             <  2:434050/100‑1 (MQ=255)
                                                                                        cGGCACCGTCACGACGGTGTCGCAGCGTAATTTTTCGGCGGGTGTTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTg             <  1:2181125/100‑1 (MQ=255)
                                                                                         ggCACCGTCACGACGGTGTCGCAGCGTAATTTTTCGGCGGGTGTTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATAtttttac            <  1:604421/100‑3 (MQ=255)
                                                                                         ggCACCGTCACGACGGTGTCGCAGCGTAATTTTTCGGCGGGTGTTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGc            <  2:211392/100‑1 (MQ=255)
                                                                                         ctcACCGTCACGACGGTGTCGCAGCGTAATTTTTCGGCGGGTGTTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTg             <  1:1318883/97‑1 (MQ=255)
                                                                                           cACCGTCACGACGGTGTCGCAGCGTACTTTTTCGGCGGGTGTTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTgaga          >  2:1318889/1‑97 (MQ=255)
                                                                                           cACCGTCACGACGGTGTCGCAGCGTAATTTTTCGGCGGGTGTTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCt           >  1:2131121/1‑99 (MQ=255)
                                                                                            aCCGTCACGACGGTGTCTCATCGTAATTTTTCGGCGGGTGTTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCTAt         <  1:3427404/100‑1 (MQ=255)
                                                                                            aCCGTAACGACGGTGTCGCCGCGTTATTTTTCGGAGGATGTTCAATCTGGATTGTCGTAATAGACAGCAACCTATTACTTCGACACaaaaaattcg             <  1:2155242/96‑9 (MQ=255)
                                                                                                tCACGACGGTGTCGCAGCGTAATTTTTCGGCGGGTGTTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTAc     <  2:1166105/100‑1 (MQ=255)
                                                                                                   cgacgTTGTCGCAGCGTAAGTTTTCGGCGGGTGTTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCca  <  2:2152723/100‑1 (MQ=255)
                                                                                                   cgacgGTGTCGCAGCGTAATTTTTCGGCGGGTGTTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTAccc   >  2:1255291/1‑99 (MQ=255)
                                                                                                   cgacgGTGTCGCAGCGTAATTTTTCGGCGGGTGTTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCca  >  1:996169/1‑100 (MQ=255)
                                                                                                   cgacgGTGTCGCAGCGTAATTTTTCGGCGGGTGTTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCca  <  1:1524314/100‑1 (MQ=255)
                                                                                                   cgacgGTGTCGCAGCGTAATTTTTCGGCGGGTGTTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCca  >  1:478356/1‑100 (MQ=255)
                                                                                                   cgacgGTGTCGCAGCGTAATTTTTCGGCGGGTGTTTCATCTGGATTGTCGTAATAGAAAGCAACCTACTCCTTCGGCACAATATTTTTGCTATCTAcc    >  1:1716860/1‑98 (MQ=255)
                                                                                                   |                                                                                                   
CTACACGAGCTACCGCCACCGCATACTGACCACCTGTAATTTCTGTCAGAATGACGCCCTCACTGTTTTCGGGAAGCGTAAAGTAACCCGGCACCGTCACGACGGTGTCGCAGCGTAATTTTTCGGCGGGTGTTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCA  >  NC_000913/2080959‑2081157

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑