BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	2,810,760	T→C	100%	intergenic (+81/‑10)	ygaH → / → mprA	putative L‑valine exporter, norvaline resistance protein/transcriptional repressor of microcin B17 synthesis and multidrug efflux

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,810,760	0	T	C	100.0%	473.4 / ‑6.7	135	intergenic (+81/‑10)	ygaH/mprA	putative L‑valine exporter, norvaline resistance protein/transcriptional repressor of microcin B17 synthesis and multidrug efflux
Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (74/59); minor base A (1/1); total (75/60)

Only 100 of 135 total aligned reads displayed.

TGGCGATTATATAACCCACAAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCTTCT  >  NC_000913/2810666‑2810855

tGGCGATTATATAACCCACAAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGGCCATAc                                                                                            <  2:3268631/100‑1 (MQ=255)

tGGCGATTATATAACCCACAAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAAGCCATAc                                                                                            >  1:3241561/1‑100 (MQ=255)

 ggCGATTATATAACCCACAAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGGCCATAcc                                                                                           >  2:588134/1‑100 (MQ=255)

 ggCGATTATATAACCCACAAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGGCCATAcc                                                                                           <  2:1618469/100‑1 (MQ=255)

 ggCGATTATATAACCCACAAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGGCCATAcc                                                                                           >  2:163649/1‑100 (MQ=255)

   cGATTATATAACCCACAAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGGCCATACCCa                                                                                         >  1:463126/1‑100 (MQ=255)

    gATTATATAACCCACAAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGGCCATACCCaa                                                                                        >  1:2234640/1‑100 (MQ=255)

       tatataACCCACAATAATCATTTTTCTAAAACAATACTTTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGGCCATACCCAAATg                                                                                     <  1:2044915/100‑1 (MQ=255)

       tatataACCCAAAAGAATCATTTTTCTAAAACAATACATTTACTTTATGAGTCACTGACGTTAATATATCGACTGAAATAAATGAGGCCATACCCAAATg                                                                                     <  1:931977/100‑1 (MQ=255)

       cccataACCCACAAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGGCCATACCCAAATg                                                                                     <  2:1559179/97‑1 (MQ=255)

        atataACCCACAAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGGCCATACCCAAATgg                                                                                    >  2:2621872/1‑100 (MQ=255)

           taACCCACAAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGGCCATACCCAAATGGATa                                                                                 >  2:2953516/1‑100 (MQ=255)

            aaCCCACAAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGGCCATACCCAAATGGATAg                                                                                >  2:758667/1‑100 (MQ=255)

            aaCCCACAAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGGCCATACCCAAATGGATAg                                                                                >  2:2798225/1‑100 (MQ=255)

             aCCCACAAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGGCCATACCCAAATGGATAGt                                                                               >  2:2364439/1‑100 (MQ=255)

               ccACAAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGGTACTGTATCTGCTGAAATTAATGAGGCCATACCCAAAGGGATAGTTc                                                                             >  2:2949951/1‑100 (MQ=255)

                cacaAGAATCATTTTTCTAAACCAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGATATTAATGAGGCCATACCCAAATGGATAGTTCg                                                                            >  1:2960183/1‑100 (MQ=255)

                cacaAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGGCCATACCCAAATGGATAGTTCg                                                                            >  1:3698960/1‑100 (MQ=255)

                 acaAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGGCCATACCCAAATGGATAGTTCGt                                                                           >  1:618300/1‑100 (MQ=255)

                  caAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGGCCATACCCAAATGGATAGTTCGtt                                                                          >  1:1377847/1‑100 (MQ=255)

                    aGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGGCCATACCCAAATGGATAGTTCGTTTa                                                                        <  2:2143932/100‑1 (MQ=255)

                       aTCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGGCCATACCCAAATGGATAGTTCGTTTACGc                                                                     <  2:1323642/100‑1 (MQ=255)

                         naTTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCATTACTATATCGGCTGAAATGAATGAGGCCATACCCAAATGGATAGTTCGTTTCCGc                                                                     >  1:2015928/2‑98 (MQ=255)

                          aTTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGGCCATACCCAAATGGATAGTTCGTTTACGCCCa                                                                  >  2:3222284/1‑100 (MQ=255)

                             tttCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGGCCATACCCAAATGGATAGTTCGTTTACGCCCATTg                                                               <  1:2314545/100‑1 (MQ=255)

                              ttCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGGCCATACCCAAATGGATAGTTCGTTTACGCCCATTGa                                                              >  1:3178568/1‑100 (MQ=255)

                              tacTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGGCCATACCCAAATGGATAGTTCGTTTACGCCCATTGa                                                              <  1:2238204/98‑1 (MQ=255)

                                cTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATGTCGGCTGAAATTAATGAGGCCATACCCAAATGGATAGTTCGTTTACGCCCATTGAAc                                                            <  1:1080493/100‑1 (MQ=255)

                                cTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGGCCATACCCAAATGGATAGTTCGTTTACGCCCATTGAAc                                                            <  2:3483344/100‑1 (MQ=255)

                                 tAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGGCCATACCCAAATGGATAGTTCGTTTACGCCCATTGAAc                                                            <  2:203913/99‑1 (MQ=255)

                                 tAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGGCCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACa                                                           >  2:528689/1‑100 (MQ=255)

                                 tAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGGCCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACa                                                           >  2:3635117/1‑100 (MQ=255)

                                 tAAAACAATACATTTACATTATTTGTTACTCTCAGTACTATATCTGATCAAATTAAGGAGTACATACCGAAATGTAAAGTTCGTTTACGACCATTGaat                                                            >  1:2426143/1‑98 (MQ=255)

                                  aaaaCAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGGCCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACaa                                                          >  2:1146828/1‑100 (MQ=255)

                                  aaaaCAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGGCCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACaa                                                          >  1:2294859/1‑100 (MQ=255)

                                  aaaaCAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGGCCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACaa                                                          >  1:368276/1‑100 (MQ=255)

                                   aaaCAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGGCCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACaaa                                                         <  2:1393446/100‑1 (MQ=255)

                                     aCAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGGCCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATg                                                       <  2:1146814/100‑1 (MQ=255)

                                     aCAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGGCCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATg                                                       <  2:1010768/100‑1 (MQ=255)

                                     aCAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGGCCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATg                                                       <  1:529148/100‑1 (MQ=255)

                                      cAATACATTTACTTTATGTGTCACTGTCGTTATTATGTCGGCTGAAATTACTGAGGCTATACCCAAATGGTTAGTTTGTTTACGCGCATTGAACGAAt                                                        <  1:158936/98‑1 (MQ=255)

                                       aaTACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGGCCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGc                                                      <  1:1123359/99‑1 (MQ=255)

                                          aCATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGGCCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTaaa                                                  <  2:2339652/100‑1 (MQ=255)

                                               tACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGGCCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTc                                             >  1:3466751/1‑100 (MQ=255)

                                                aCTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATTAGGCCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTcg                                            >  1:1911986/1‑100 (MQ=255)

                                                aCTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGGCCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTcg                                            <  1:626524/100‑1 (MQ=255)

                                                aCTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGGCCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTcg                                            <  1:1310061/100‑1 (MQ=255)

                                                aCTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGGCCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTcg                                            <  1:2508236/100‑1 (MQ=255)

                                                aCTTTATTTGTCACTATCGTTACTATATCGGCTGAAATTAATGAGGCCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTcg                                            <  1:143801/100‑1 (MQ=255)

                                                 cTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGGCCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTcgc                                           <  2:1634222/100‑1 (MQ=255)

                                                 cTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGGCCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTcgc                                           >  2:2722184/1‑100 (MQ=255)

                                                   ttATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGGCCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCgcgc                                         >  1:580503/1‑100 (MQ=255)

                                                    tATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGGCCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCgcgc                                         >  1:1664053/1‑99 (MQ=255)

                                                    tATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGGCCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGcc                                        >  2:2319672/1‑100 (MQ=255)

                                                        tGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGGCCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAgcc                                    >  1:2554077/1‑100 (MQ=255)

                                                         gTCACTGTCGTTACTATATCGGCTGAAATTAATGAGGCCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAgccg                                   <  1:2613455/100‑1 (MQ=255)

                                                         gTCACTGTCGTTACTATATCGGCTGAAATTAATGAGGCCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAgccg                                   >  1:2648028/1‑100 (MQ=255)

                                                           cACTGTCGTTACTATATCGGCTGAAATTAGTGAGGCCATACCCAAATGGATAGTTCGTTTACGCCCCTTGAACAAATGCTAAAATTTCGCGCCAgccgcc                                 <  1:2226293/100‑1 (MQ=255)

                                                            aCTGTCGTTACTATATCGGCTGAAATTAATGAGGCCATACCCAAATGGATGGTTCGTTTACGCCCATTGAACAAATGCTAAGATTTCGCGCCAgccgcc                                 <  1:18598/99‑1 (MQ=255)

                                                            aCTGTCGTTACTATATCGGCTGAAATTAATGAGGCCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCa                                <  1:1263047/100‑1 (MQ=255)

                                                              tGTCGTTACTATATCGGCTGAAATTAATGAGGCCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACg                              >  2:2859871/1‑100 (MQ=255)

                                                               gTCGTTACTATATCGGCTGAAATTAATGAGGCCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGa                             >  2:3227049/1‑100 (MQ=255)

                                                                 cGTTACTATATCGGCTGAAATTAATGAGGCCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAg                           <  2:876798/100‑1 (MQ=255)

                                                                  gTTCCTATATCGGCTGAGATTAATGAGGCCAGACCCAAATGGATAGTGCGTTTACGCCCATTGAACAAACGCTAAAAGTTCGCGCCAGCCGCCACGaa                            >  1:2779803/1‑98 (MQ=255)

                                                                  gTTACTATATCGGCTGAAATTAATGAGGCCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGa                          >  1:3060916/1‑100 (MQ=255)

                                                                  gTTACTATATCGGCTGAAATTAATGAGGCCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGa                          <  2:2560698/100‑1 (MQ=255)

                                                                  gTTACTATATCGGCTGAAATTAATGAGGCCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCACGCCAGCCGCCACGAAGa                          >  1:3139236/1‑100 (MQ=255)

                                                                   ttACTATATCGGCTGAAATTAATGAGGCCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGAt                         >  1:394364/1‑100 (MQ=255)

                                                                    tACTATATCGGCTGAAATTAATGAGGCCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGAtt                        >  2:2982493/1‑100 (MQ=255)

                                                                     aCTATATCGGCTGAAATTAATGAGGCCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGAttt                       >  1:809968/1‑100 (MQ=255)

                                                                     aCTATATCGGCTGAAATTAATGAGGCCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGAttt                       >  2:610886/1‑100 (MQ=255)

                                                                     aCTATATCGGCTGAAATTAATGAGGCCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGAttt                       >  1:3554612/1‑100 (MQ=255)

                                                                      cTATATCGGCTGAAATTAATGAGGCCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGAtttt                      <  2:1569703/100‑1 (MQ=255)

                                                                        atatCGGCTGAAATTAATGAGGCCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTcc                    <  1:504023/100‑1 (MQ=255)

                                                                        atatCGGCTGAAATTAATGAGGCCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTcc                    >  1:3376219/1‑100 (MQ=255)

                                                                        atatCGGCTGAAATTAATGAGGCCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTcc                    <  2:3271798/100‑1 (MQ=255)

                                                                        atatCGGCTGAAATTAATGAGGCCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTcc                    <  2:1784275/100‑1 (MQ=255)

                                                                        atatCGGCTGAAATTAATGAGGCCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTcc                    <  1:1913300/100‑1 (MQ=255)

                                                                         tatCGGCTGAAATTAATGAGGCCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCt                   <  2:1324200/100‑1 (MQ=255)

                                                                         tatCGGCTGAAATTAATGAGGCCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCt                   <  2:2162947/100‑1 (MQ=255)

                                                                            cGGCTGAAATTAATGAGGCCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTAt                >  1:363427/1‑100 (MQ=255)

                                                                             ggCTGAAATTAATGAGGCCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATc               <  1:3406433/100‑1 (MQ=255)

                                                                              gCTGAAATTAATGAGGCCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCa              >  1:2132631/1‑100 (MQ=255)

                                                                              gCTGAAATTAATGAGGCCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCa              >  2:3286048/1‑100 (MQ=255)

                                                                               cTGAAATTAATGAGGCCATACCCAAATGGATAGTTCGTTTAGGCCCAGTGAACAAATGCTAGAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATc               >  1:2014609/1‑98 (MQ=255)

                                                                                tGAAATTAATGAGGCCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAACTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAgg            >  2:2598613/1‑100 (MQ=255)

                                                                                 gAAATTAATGAGGCCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGga           >  1:2166959/1‑100 (MQ=255)

                                                                                 gAAATTAATGAGGCCATACCCAAATGGATAGTTCGTTTACGCCCAAAGAACAAATGCTAAAATTTCGCGCCAGCAGCCACGAAGATTTTCCTTATCAg             >  1:1731709/1‑98 (MQ=255)

                                                                                  aaaTTAATGAGGCCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGgag          >  2:3241314/1‑100 (MQ=255)

                                                                                   aaTTAATGAGGCCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGgaga         >  2:2841833/1‑100 (MQ=255)

                                                                                   aaTTAATGAGGCCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGgaga         >  2:1870475/1‑100 (MQ=255)

                                                                                      tAATGAGGCCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATcc      >  1:2326877/1‑100 (MQ=255)

                                                                                       aaTGAGGCCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATcc      >  2:490459/1‑99 (MQ=255)

                                                                                        aTGAGGCCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCtt    >  2:218636/1‑100 (MQ=255)

                                                                                         tGAGGCCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCTTc   <  1:3246710/100‑1 (MQ=255)

                                                                                          gAGGCCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCTTCt  <  1:1832791/100‑1 (MQ=255)

                                                                                          gAGGCCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCTTCt  <  2:2733654/100‑1 (MQ=255)

                                                                                          gAGGCCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCTTCt  <  1:1071395/100‑1 (MQ=255)

                                                                                          gAGGCCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCTTCt  <  2:1235908/100‑1 (MQ=255)

                                                                                           aGGACATACCCAAATGGATAGCTCGTTTACGCCCATTGAACAAATGCTAAAACTTCGCGCCAGCCGCCACGAAGATTTCCCTTATCAGGAGATCCTTCt  <  1:1306685/99‑1 (MQ=255)

TGGCGATTATATAACCCACAAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCTTCT  >  NC_000913/2810666‑2810855

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`