Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,273,229 A→T 15.3% intergenic (+27/+17) chaC → / ← ychN cation transport regulator/putative sulfur relay protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,273,2290AT15.3% 354.0 / 42.8 130intergenic (+27/+17)chaC/ychNcation transport regulator/putative sulfur relay protein
Reads supporting (aligned to +/- strand):  ref base A (52/57);  new base T (11/9);  total (64/66)
Fisher's exact test for biased strand distribution p-value = 6.30e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01

Only 100 of 132 total aligned reads displayed.

TGCTGGTATCGGTAAAAAAACTGCTGGCGGAGAATTTTCCGGATGGTGTGTTACGTCCGGGATTCGCCTGAGTAAACTTCCCGCATAGTGGGGCGTCAGACGCCCCCTCAAACATTAAAATGTGAGCACTTTATCGGCTGACAGCGTCCATTGCGCCAGTTCCACAAGAGTACCGATTTCCACCCCATCAATCAGAG  >  NC_000913/1273132‑1273328
                                                                                                 |                                                                                                   
 gCTGGTATCGGTAAAAAAACTGCTGGCGGAGAATTTTCCGGATGGTGTGTTACGTCCGGGATTCGCCTGAGTAAACTTCCCGCATAGTGGGGCGTCAGAc                                                                                                  <  1:3550787/100‑1 (MQ=255)
   tGGTATCGGTAAAAAAACTGCTGGCGGAGAATTTTCCGGATGGTGTGTTACGTCCGGGATTCGCCTGAGTAAACTTCCCGCATAGTGGGGCGTCAGACGc                                                                                                <  1:361324/100‑1 (MQ=255)
    ggTATCGGTAAAAAAACTGCTGGCGGAGAATTTTCCGGATGGTGTGTTACGTCCGGGATTCGCCTGAGTAAACTTCCCGCATAGTGGGGCGTCAGACGcc                                                                                               >  2:3338830/1‑100 (MQ=255)
      tATCGGTAAAAAAACTGCTGGCGGAGAATTTTCCGGATGGTGTGTTACGTCCGGGATTCGCCTGAGTAAACTTCCCGCATAGTGGGGCGTCTGACGcccc                                                                                             <  2:2075495/100‑1 (MQ=255)
      tATCGGTAAAAAAACTGCTGGCGGAGAATTTTCCGGATGGTGTGGTACGTCCGGGATTCGCCTGAGTAAACTTCCCGCATAGTGGGGCGTCTGACGcccc                                                                                             <  2:2393687/100‑1 (MQ=255)
       cgatctAAAAAAACTGCTGGCGGAGAATTTTCCGGATGGTGTGTTACGTCCGGGATTCGCCTGAGTAAACTTCCCGCATAGTGGGGCGTCAGACGccccc                                                                                            <  2:570340/95‑1 (MQ=255)
        tCGGTAAAAAAACTGCTGGCGGAGAATTTTCCGGATGGTGTGTTACGTCCGGGATTCGCCTGAGTAAACTTCCCGCATAGTGGGGCGTCTGACGccccac                                                                                           <  2:3664987/100‑3 (MQ=255)
        tCGGTAAAAAAACTGCTGGCGGAGAATTTTCCGGATGGTGTGTTACGTCCGGGATTCGCCTGAGTAAACTTCCCGCATAGTGGGGCGTCAGACGCCCCCt                                                                                           >  2:1113382/1‑100 (MQ=255)
          ggcaaaaaaaCAGCTTGCGGAGAATTTTCTGAATGGTGACAAACGTCCGGGATTCGCCTGAGTAAAGTTCCCGCATCGTGCGCCGTCCGCCGccccc                                                                                            >  1:3478459/4‑97 (MQ=255)
          ggTAAAAACACTGCTGGCGGAGATTTTTCCGGATGGTGTGTTACGTCCGGGATTCGCCTGAGTAAACTTCCCGCATAGTGGGGCGTCTGACGccccac                                                                                           <  1:12404/98‑3 (MQ=255)
           gTAAAAAAACTGCTGGCGGAGAATTTTCCGGATGGTGTGTTACGTCCGGGATTCGCCTGAGTAAACTTCCCGCATAGTGGGGCGTCAGACGCCCCCTCaa                                                                                        >  2:3627507/1‑100 (MQ=255)
            tAAAAGAACGGCTGCCGGATAGTTGTTCGGATGGGGTGTGACGTCGGGGATTCGCCTGAGGAAAAGTCCCGCATAGTGGGGGGTCTGACGccccacta                                                                                         <  1:3514844/98‑5 (MQ=255)
            tAAAAAAACTGCTGGCGGAGAATTTTCCGGATGGTGTGTTACGTCCGGGATTCGCCTGAGTAAACTTCCCGCATAGTGGGGCGTCAGACGCCCCCTCaaa                                                                                       >  1:155568/1‑100 (MQ=255)
             aaaaaaaCTGCTGGCGGAGAATTTTCCGGATGGTGTGTTACGTCCGGGATTCGCCTGAGTAAACTTCCCGCATAGTGGGGCGTCAGACGcccccagatcg                                                                                      >  1:570337/1‑94 (MQ=255)
             aaaaaaaCTGCTGGCGGAGAATTTTCCGGATGGTGTGTTACGTCCGGGATTCGCCTGAGTAAACTTCCCGCATAGTGGGGCGTCAGACGCCCCCTCAAAc                                                                                      <  2:3045813/100‑1 (MQ=255)
             aaaaaaaCTGCTGGCGGAGAATTTTCCGGATGGTGTGTTACGTCCGGGATTCGCCTGAGTAAACTTCCCGCATAGTGGGGCGTCAGACGCCCCCTCAAAc                                                                                      <  2:920049/100‑1 (MQ=255)
             aaaaaaaCTGCTGGCGGAGAATTTTCCGGATGGTGTGTTACGTCCGGGATTCGCCTGAGTAAACTTCCCGCATAGTGGGGCGTCAGACGCCCCCTCAAAc                                                                                      <  2:2117666/100‑1 (MQ=255)
              aaaaaaCTGCTGGCGGAGAATTTTCCGGATGGTGTGTTACGTCCGGGATTCGCCTGAGTAAACTTCCCGCATAGTGGGGCGTCTGACGccccactatgcg                                                                                     <  2:2957683/100‑9 (MQ=255)
              aaaaaaCTGCTGGCGGAGAATTTTCCGGATGGTGTGTTACGTCCGGGATTCGCCTGAGTAAACTTCCCGCATAGTGGGGCGTCAGACGCCCCCTCAAACa                                                                                     >  2:3234448/1‑100 (MQ=255)
               aaaaaCTGCTGGCGGAGAATTTTCCGGATGGTGTGTTACGTCCGGGATTCGCCTGAGTAAACTTCCCGCATAGTGGGGCGTCTGACGccccactatgcgg                                                                                    <  2:1071189/100‑10 (MQ=39)
               aaaaaCTGCTGGCGGAGAATTTTCCGGATGGTGTGTTACGTCCGGGATTCGCCTGAGTAAACTTCCCGCATAGTGGGGCGTCAGACGCCCCCTCAAACAt                                                                                    <  2:2699038/100‑1 (MQ=255)
                aaaaCTGCTGGCGGAGAATTTTCCGGATGGTGTGTTACGTCCGGGATTCGCCTGAGTAAACTTCCCGCATAGTGGGGCGTCAGACGCCCCCTCAAACAtt                                                                                   <  2:44911/100‑1 (MQ=255)
                 aaaCTGCTGGCGGAGAATTTTCCGGATGGTGTGTTACGTCCGGGATTCGCCTGAGTAAACTTCCCGCATAGTGGGGCGTCAGACGCCCCCTCAAACATTa                                                                                  <  2:2181988/100‑1 (MQ=255)
                   aCTGCTGGCGGAGAATTTTCCGGATGGTGTGTTACGTCCGGGATTCGCCTGAGTAAACTTCCCGCATAGTGGGGCGTCAGACGCCCCCTCAAACATTaaa                                                                                <  1:2526261/100‑1 (MQ=255)
                       ctgGCGGAGAATTTTCCGGATGGTGTGTTACGTCCGGGATTCGCCTGAGTAAACTTCCCGCATAGTGGGGCGTCAGACGCCCCCTCAAACATTAAAAtgt                                                                            >  1:48483/1‑100 (MQ=255)
                        tgGCGGAGGATTTTCCGGATGGTGTGTTACGTCCGGGATTCGCCTGAGTAAACTTCCCGCATAGTGGGGCGTCAGACGCCCCCTCAAACATTAAAAtgtg                                                                           <  2:63166/100‑1 (MQ=255)
                         ggCGGAGAATTTTCCGGATGGTGTGTTACGTCCGGGATTCGCCTGAGTAAACTTCCCGCATAGTGGGGCGTCAGACGCCCCCTCAAACATTAAAATGTGa                                                                          >  1:2619364/1‑100 (MQ=255)
                         ggCGGAGAATTTTCCGGATGGTGTGTTACGTCCGGGATTCGCCTGAGTAAACTTCCCGCATAGTGGGGCGTCAGACGCCCCCTCAAACATTAAAATGTGa                                                                          >  2:3406469/1‑100 (MQ=255)
                          gCGTAGAATTTTCCGGATGTTGTGTTACGTCCGGGATTCGCCTGAGTAAACTTCCCGCATAGTGTGGCGTCAGACGCCCCCTCAAACATTAAAATGTTAg                                                                         <  1:1379333/100‑1 (MQ=255)
                           cGGAGAATTTTCCGGATGGTGTGTTACGTCCGGGATTCGCCTGAGTAAACTTCCCGCATAGTGGGGCGTCAGCCGCCCCCTCAAACATTAAAATGTGAGc                                                                        >  1:2472531/1‑100 (MQ=255)
                           cGGAGAATTTTCCGGATGGTGTGTTACGTCCGGGATTCGCCTGAGTAAACTTCCCGCATAGTGGGGCGTCAGACGCCCCCTCAAACATTAAAATGTGAGc                                                                        >  2:92930/1‑100 (MQ=255)
                           cGGAGAATTTTCCGGATGGTGTGTTACGTCCGGGATTCGCCTGAGTAAACTTCCCGCATAGTGGGGCGTCAGACGCCCCCTCAAACATTAAAATGTGAGc                                                                        >  2:3098972/1‑100 (MQ=255)
                                aaTTTTCCGGATGGTGTGTTACGTCCGGGATTCGCCTGAGTAAACTTCCCGCATAGTGGGGCGTCAGACGCCCCCTCAAACATTAAAATGTGAGCACttt                                                                   <  2:3180884/100‑1 (MQ=255)
                                 aTTTTCCGGATGGTGTGTTACGTCCGGGATTCGCCTGAGTAAACTTCCCGCATAGTGGGGCGTCAGACGCCCCCTCAAACATTAAAATGTGAGCACTTTa                                                                  <  1:299456/100‑1 (MQ=255)
                                  ttttCCGGATGGTGTGTTACGTCCTGGATTCTCCTGAGTAAACGTCCCGCATAGTGGGGCGTCAGACGCCCCCTCAAACATTAAAATGTGAGCACTTTAt                                                                 >  1:3326805/1‑100 (MQ=255)
                                  ttttCCGGATGGTGTGTTACGTCCGGGATTCGCCTGAGTAAACTTCCCGCATAGTGGGGCGTCAGACGCCCCCTCAAACATTAAAATGTGAGCACTTTAt                                                                 >  1:3370828/1‑100 (MQ=255)
                                   tttCCGGATGGTGTGTTACGTCCGGGATTCGCCTGAGTAAACTTCCCGCATAGTGGGGCGTCAGACGCCCCCTCAAACATTAAAATGTGAGCACTTTATc                                                                <  1:42727/100‑1 (MQ=255)
                                     tCCGGATGGTGTGTTACGTCCGGGATTCGCCTGAGTAAACTTCCCGCATAGTGGGGCGTCAGACGCCCCCTCAAACATTAAAATGTGAGCACTTTATCgg                                                              <  1:2507498/100‑1 (MQ=255)
                                     tCCGGATGGTGTGTTACGTCCGGGATTCGCCTGAGTAAACTTCCCGCATAGTGGGGCGTCAGACGCCCCCTCAAACATTAAAATGTGAGCACTTTATCgg                                                              <  1:1030673/100‑1 (MQ=255)
                                       cGGATGGTGTGTTACGTCCGGGATTCGCCTGAGTAAACTTCCCGCATAGTGGGGCGTCAGACGCCCCCTCAAACATTAAAATGTGAGCACTTTATCGGCt                                                            >  2:3424013/1‑100 (MQ=255)
                                           tGGTGTGTTACGTCCGGGATTCGCCTGAGTAAACTTCCCGCATAGTGGGGCGTCAGACGCCCCCTCAAACATTAAAATGTGAGCACTTTATCGGCTGACa                                                        <  1:1450146/100‑1 (MQ=255)
                                             gtgtgtTACGTCCGGGATTCGCCTGAGTAAACTTCCCGCATAGTGGGGCGTCAGACGCCCCCTCAAACATTAAAATGTGAGCACTTTATCGGCTGACAGc                                                      <  2:3618656/100‑1 (MQ=255)
                                               gtgtTACGTCCGGGATTCGCCTGAGTAAACTTCCCGCATAGTGGGGCGTCAGACGCCCCCTCAAACATTAAAATGTGAGCACTTTATCGGCTGACAGCGt                                                    <  2:84064/100‑1 (MQ=255)
                                               gtgtTACGTCCGGGATTCGCCTGAGTAAACTTCCCGCATAGTGGGGCGTCAGACGCCCCCTCAAACATTAAAATGTGAGCACTTTATCGGCTGACAGCGt                                                    <  2:908551/100‑1 (MQ=255)
                                                tgtTACGTCCGGGATTCGCCTGAGTAAACTTCCCGCATAGTGGGGCGTCAGACGCCCCCTCAAACATTAAAATGTGAGCACTTTATCGGCTGACAGCGTc                                                   >  2:344927/1‑100 (MQ=255)
                                                  ttACGTCCGGGATTCGCCTGAGTAAACTTCCCGCATAGTGGGGCGTCAGACGCCCCCTCAAACATTAAAATGTGAGCACTTTATCGGCTGACAGCGTCCa                                                 <  1:1063363/100‑1 (MQ=255)
                                                   tACGTCCGGGATTCGCCTGAGTAAACTTCCCGCATAGTGGGGCGTCAGACGCCCCCTCAAACATTAAAATGTGAGCACTTTATCGGCTGACAGCGTCCAt                                                >  1:1661296/1‑100 (MQ=255)
                                                     cGTCCGGGATTCGCCTGAGTAAACTTCCCGCATAGTGGGGCGTCAGACGCCCCCTCAAACATTAAAATGTGAGCACTTTATCGGCTGACAGCGTCCATTg                                              <  1:3638038/100‑1 (MQ=255)
                                                      ntCCGGGATTCGCCTGAGTAAACTTCCCGCATAGTGGGGCGTCAGACGCCCCCTCAAACATTAAAATGTGAGCACTTTATCGGCTGACAGCGTCCATTgc                                             >  1:1877986/2‑100 (MQ=255)
                                                      gTCCGGGATTCGCCTGAGTAAACTTCCCGCATAGTGGGGCGTCAGACGCCCCCTCAAACATTAAAATGTGAGCACTTTATCGGCTGACAGCGTCCATTgc                                             >  2:2611548/1‑100 (MQ=255)
                                                       tCCGGGATTCGCCTGAGTAAACTTCCCGCATAGTGGGGCGTCAGACGCCCCCTCAAACATTAAAATGTGAGCACTTTATCGGCTGACAGCGTCCATTgcg                                            <  1:3021324/100‑1 (MQ=255)
                                                       tCCGGGATTCGCCTGAGTAAACTTCCCGCATAGTGGGGCGTCAGACGCCCCCTCAAACATTAAAATGTGAGCACTTTATCGGCTGACAGCGTCCATTgcg                                            <  1:3635390/100‑1 (MQ=255)
                                                       tCCGGGATTCGCCTGAGTAAACTTCCCGCATAGTGGGGCGTCAGACGCCCCCTCAAACATTAAAATGTGAGCACTTTATCGGCTGACAGCGTCCATTgcg                                            <  2:1818795/100‑1 (MQ=255)
                                                       tCCGGGATTCGCCTGAGTAAACTTCCCGCATAGTGGGGCGTCAGACGCCCCCTCAAACATTAAAATGTGAGCACTTTATCGGCTGACAGCGTCCATTgcg                                            <  2:2842698/100‑1 (MQ=255)
                                                        ccGGGATTCGCCTGAGTAAACTTCCCGCATAGTGGGGCGTCAGACGCCCCCTCAAACATTAAAATGTGAGCACTTTATCGGCTGACAGCGTCCATTgcgc                                           <  2:761603/100‑1 (MQ=255)
                                                         cGGGATTCGCCTGAGTAAACTTCCCGCATAGTGGGGCGTCAGACGCCCCCTCAAACATTAAAATGTGAGCACTATATCGGCTGACAGCGTCCATTGCGcc                                          <  2:2246316/100‑1 (MQ=255)
                                                          gggATTCGCCTGAGTAAACTTCCCGCATAGTGGGGCGTCAGACGCCCCCTCAAACATTAAAATGTGAGCACTTTATCGGCTGACAGCGTCCATTGCGCCa                                         <  2:1379097/100‑1 (MQ=255)
                                                            gATTCGCCTGAGTAAACTTCCCGCATAGTGGGGCGTCAGACGCCCCCTCAAACATTAAAATGTGAGCACTTTATCGGCTGACAGCGTCCATTGCGCCAGt                                       >  2:380197/1‑100 (MQ=255)
                                                              ttCGCCTGAGTAAACTTCCCGCATAGTGGGGCGTCAGACGCCCGCTCAAACATTCAAATGGGAGCACTTTATCGGCTTCCAGCGTCCATTGCGCCCGTTc                                     >  1:806122/1‑100 (MQ=255)
                                                               tCGCCTGAGTAAACTTCCCGCATAGTGGGGCGTCAGACGCCCCCTCAAACATTAAAATGTGAGCACTTTATCGGCTGACAGCGTCCATTGCGCCAGTTcc                                    <  2:2168043/100‑1 (MQ=255)
                                                                cGCCTGAGTAAACTTCCCGCATAGTGGGGCGTCAGACGCCCCCTCAAACATTAAAATGTGAGCACTTTATCGGCTGACAGCGTCCATTGCGCCAGTTCca                                   <  2:142028/100‑1 (MQ=255)
                                                                 gCCTGAGTAAACTTCCCGCATAGTGGGGCGTCAGACGCCCCCTCAAACATTAAAATGTGAGCACTTTATCGGCTGACAGCGTCCATTGCGCCAGTTCcac                                  <  2:121158/100‑1 (MQ=255)
                                                                    tGAGTAAACTTCCCGCATAGTGGGGCGTCAGACGCCCCCTCAAACATTAAAATGTGAGCACTTTATCGGCTGACAGCGTCCATTGCGCCAGTTCCACAag                               >  1:2812499/1‑100 (MQ=255)
                                                                     gAGTAAACTTCCCGCATAGTGGGGCGTCAGACGCCCCCTCAAACATTAAAATGTGAGCACTTTATCGGCTGACAGCGTCCATTGCGCCAGTTCCACAaga                              >  2:3126781/1‑100 (MQ=255)
                                                                      aGTAAACTTCCCGCATAGTGGGGCGTCAGACGCCCCCTCAAACATTAAAATGTGAGCACTTTATCGGCTGACAGCGTCCATTGCGCCAGTTCCACAagag                             >  2:1854218/1‑100 (MQ=255)
                                                                      aGTAAACTTCCCGCATAGTGGGGCGTCAGACGCCCCCTCAAACATTAAAATGTGAGCACTTTATCGGCTGACAGCGTCCATTGCGCCAGTTCCACAagag                             <  1:1290794/100‑1 (MQ=255)
                                                                       gTAAACTTCCCGCATAGTGGGGCGTCAGACGCCCCCTCAAACATTAAAATGTGAGCACTTTATCGGCTGACAGCGTCCATTGCGCCAGTTCCACAAGAGt                            >  2:2871229/1‑100 (MQ=255)
                                                                           aCTTCCCGCATAGTGGGGCGTCAGACGCCCCCTCAAACATTAAAATGTGAGCACTTTATCGGCTGACAGCGTCCATTGCGCCAGTTCCACAAGAGTACCg                        <  1:1770193/100‑1 (MQ=255)
                                                                            cTTCCCGCATAGTGGGGCGTCAGACGCCCCCTCAAACATTAAAATGTGAGCACTTTATCGGCTGACAGCGTCCATTGCGCCAGTTCCACAAGAGTACCGa                       >  1:511791/1‑100 (MQ=255)
                                                                             ttCCCGCATAGTGGGGCGTCAGACGCCCCCTCAAACATTAAAATGTGAGCACTTTATCGGCTGACAGCGTCCATTGCGCCAGTTCCACAAGAGTACCGAt                      <  2:2912277/100‑1 (MQ=255)
                                                                               aatgtttgagggggCGTCTGACGCCCCCTCAAACATTAAAATGTGAGCACTTTATCGGCTGACAGCGTCCATTGCGCCAGTTCCACAAGAGTACCGAttt                    >  2:282329/11‑100 (MQ=38)
                                                                                ccGCATAGTGGGGCGTCAGACGCCCCCTCAAACATTAAAATGTGAGCACTTTATCGGCTGACAGCGTCCATTGCGCCAGTTCCACAAGAGTACCGATTTc                   >  2:1683793/1‑100 (MQ=255)
                                                                                ccGCATAGTGGGGCGTCAGACGCCCCCTCAAACATTAAAATGTGAGCACTTTATCGGCTGACAGCGTCCATTGCGCCAGTTCCACAAGAGTACCGATTTc                   >  2:1551798/1‑100 (MQ=255)
                                                                                atgtttgagggggCGTCTGACGCCCCCTCAACCATTAAAATGTGAGCACTCTATCGGCTGACAGCGTCCATTGCTCCAGCTCCACAAGAGGATTGATTTc                   >  1:3338337/10‑100 (MQ=25)
                                                                                 tgtttgagggggCGTCTGACGCCCCCTCAAACATTAAAATGTGAGCACTTTATCGGCTGACAGCGTCCATTGCGCCAGTTCCACAAGAGTACCGATTTcc                  >  2:541328/9‑100 (MQ=255)
                                                                                 cGCATAGTGGGGCGTCAGACGCCCCCTCAAACATTAAAATGTGAGCACTTTATCGGCTGACAGCGTCCATTGCGCCAGTTCCACAAGAGTACCGATTTcc                  >  1:1889361/1‑100 (MQ=255)
                                                                                 cGCATAGTGGGGCGTCAGACGCCCCCTCAAACATTAAAATGTGAGCACTTTATCGGCTGACAGCGTCCATTGCGCCAGTTCCACAAGAGTACCGATTTcc                  >  2:3459094/1‑100 (MQ=255)
                                                                                 cGCATAGTGGGGCGTCAGACGCCCCCTCAAACATTAAAATGTGAGCACTTTATCGGCTGACAGCGTCCATTGCGCCAGTCCCACAAGAGTACCGATTTcc                  <  1:2258272/100‑1 (MQ=255)
                                                                                 cGCATAGTGGGGCGTCAGACGCCCCCTCAAACAGTAAAATGTGAGCACTTTATCGGCTGACAGCGTCCATTGCGCCAGTTCCACAAGAGTACCGATTTcc                  <  1:1095769/100‑1 (MQ=255)
                                                                                 cGCATAGTGGGGCGTCAGACGCCACCTCAAACATTAAAATGTGAGCACTTTATCGGCTGACAGCGTCCATTGCGCGAGTTCCACAAGAGTACCGATTTcc                  >  1:892478/1‑100 (MQ=255)
                                                                                  gtttgagggggCGTCTGACGCCCCCTCAAACATTAAAATGTGAGCACTTTATCGGCTGACAGCGTCCATTGCGCCAGTTCCACAAGAGTACCGATTTCCa                 >  1:60658/8‑100 (MQ=255)
                                                                                   ttggagggggCGTCTGACGCCCCCTCAAACATTAAAATGGGGGCACGTTATCGGCTGACAGCGTCCATTGAGCCAGTTCCACACGAGTCCCGATTTCCa                 >  1:2539486/7‑99 (MQ=255)
                                                                                    aTAGTGGGGCGTCAGACGCCCCCTCAAACATTAAAATGTGAGCACTTTATCGGCTGACAGCGTCCATTGCGCCAGTTCCTCAAGAGTACCGATTTCCAcc               <  1:2256981/100‑1 (MQ=255)
                                                                                    aTAGTGGGGCGTCAGACGCCCCCTCAAACATTAAAATGTGAGCACTTTATCGGCTGACAGCGTCCATTGCGCCAGTTCCACAAGAGTACCGATTTCCAcc               <  1:3320337/100‑1 (MQ=255)
                                                                                     tgagggggCGTCTGACGCCCCCTCAAACATTAAAATGTGAGCACTTTATCGGCTGACAGCGTCCATTGCGCCAGTTCCACAAGAGTACCGATTTCCAccc              >  1:1036498/5‑100 (MQ=255)
                                                                                     tgagggggCGTCTGACGCCCCCTCAAACATTAAAATGTGAGCACTTTATCGGCTGACAGCGTCCATTGCGCCAGTTCCACAAGAGTACCGATTTCCAccc              >  2:1005966/5‑100 (MQ=255)
                                                                                      gagggggCGTCTGACGCCCCCTCAAACATTAAAATGTGAGCACTTTATCGGCTGACAGCGTCCATTGCGCCAGTTCCACAAGAGTACCGATTTCCAcccc             >  2:1492223/4‑100 (MQ=255)
                                                                                      aGTGGGGCGTCAGACGCCCCCTCAAACATTAAAATGTGAGCACTTTATCGGCTGACAGCGTCCATTGCGCCAGTTCCACAAGAGTACCGATTTCCAcccc             <  2:1322995/100‑1 (MQ=255)
                                                                                       aagaggCGTCTGACGCCCCCTCAAACATTAACATGTGAGCACCTTATCGGCTGACAGCGTCCATTCCGCCCGACCCACAAGAGTACCGCTTTCCACCCCa            >  1:905826/5‑100 (MQ=255)
                                                                                        gggggCGTCTGACGCCCCCTCAAACATTAAAATGTGAGCACTTTATCGGCTGACAGTGTCCATTGCTCCAGTTCCACAAGAGTACCGATTTCCAcccc             >  1:559882/2‑98 (MQ=255)
                                                                                         ggggCGTCAGACGCCCCCTCAAACATTAAAATGTGAGCACTTTATCGGCTGACAGCGTCCATTGCGCCAGTTCCACAAGAGTACCGATTTCCACCCCatc          >  2:3483095/1‑100 (MQ=255)
                                                                                         ggggCGTCAGACGCCCCCTCAAACATTAAAATGTGAGCACTTTATCGGCTGACAGCGTCCATTGCGCCAGTTCCACAAGAGTACCGATTTCCACCCCatc          <  2:501379/100‑1 (MQ=255)
                                                                                           ggCGTCAGACGCCCCCTCAAACATTAAAATGTGAGCACTTTATCGGCTGACAGCGTCCATTGCGCCAGTTCCACAAGAGTACCGATTTCCACCCCatcaa        >  2:1236227/1‑100 (MQ=255)
                                                                                              gTCAGACGCCCCCTCAAACATTAAAATGTGAGCACTTTATCGGCTGACAGCGTCCATTGCGCCAGTTCCACAAGAGTACCGATTTCCACCCCatcaatca     >  2:1844113/1‑100 (MQ=255)
                                                                                              gTCAGACGCCCCCTCAAACATTAAAATGTGAGCACTTTATCGGCTGACAGCGTCCATTGCGCCAGTTCCACAAGAGTACCGATTTCCACCCCatcaatca     >  2:429893/1‑100 (MQ=255)
                                                                                              gTCAGACGCCCCCTCAAACATTAAAATGTGAGCACTTTATCGGCTGACAGCGTCCATTGCGCCAGTTCCACAAGAGTACCGATTTCCACCCCatcaatca     <  2:1157627/100‑1 (MQ=255)
                                                                                              gTCAGACGCCCCCTCAAACATTAAAATGTGAGCACTTTATCGGCTGACAGCGTCCATTGCGCCAGTTCCACAAGAGTACCGATTTCCACCCCatcaatca     <  1:31981/100‑1 (MQ=255)
                                                                                               tCAGACGCCCCCTCAAACATTAAAATGTGAGCACTTTATCGGCTGACAGCGTCCATTGCGCCAGTTCCACAAGAGTACCGATTTCCACCCCATCAATCag    <  1:2865545/100‑1 (MQ=255)
                                                                                                 aGACGCCCCCTCAAACATTAAAATGTGAGCACTTTATCGGCTGACAGCGTCCATTGCGCCAGTTCCACAAGAGTACCGATTTCCACCCCATCAATCagag  <  2:1921045/100‑1 (MQ=255)
                                                                                                 aGACGCCCCCTCAAACATTAAAATGTGAGCACTTTATCGGCTGACAGCGTCCATTGCGCCAGTTCCACAAGAGTACCGATTTCCACCCCATCAATCagag  >  2:1471297/1‑100 (MQ=255)
                                                                                                 |                                                                                                   
TGCTGGTATCGGTAAAAAAACTGCTGGCGGAGAATTTTCCGGATGGTGTGTTACGTCCGGGATTCGCCTGAGTAAACTTCCCGCATAGTGGGGCGTCAGACGCCCCCTCAAACATTAAAATGTGAGCACTTTATCGGCTGACAGCGTCCATTGCGCCAGTTCCACAAGAGTACCGATTTCCACCCCATCAATCAGAG  >  NC_000913/1273132‑1273328

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: