Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,832,1880AT21.6% 210.5 / 59.7 102intergenic (+22/+31)yicH/yicIputative inner membrane‑anchored periplasmic AsmA family protein/putative alpha‑glucosidase
Reads supporting (aligned to +/- strand):  ref base A (35/45);  new base T (12/10);  total (47/55)
Fisher's exact test for biased strand distribution p-value = 4.70e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.93e-01

Only 100 of 105 total aligned reads displayed.

AGCAGCAAGTGACTCAAACCATGAATGCTGGCATCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGA  >  NC_000913/3832090‑3832287
                                                                                                  |                                                                                                   
agcagcAAGTGACTCAAACCATGAATGCTGGCATCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGAc                                                                                                    >  1:77355/1‑100 (MQ=255)
  cagcaAGTGACTCAAACCATGAATGCTGGCATCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTc                                                                                                  <  1:2267739/100‑1 (MQ=255)
   agcaAGTGACTCAAACCATGAATGCTGGCATCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCa                                                                                                 <  2:2014120/100‑1 (MQ=255)
    gcaAGTGACTCAAACCATGAATGCTGGCATCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAg                                                                                                >  1:619956/1‑100 (MQ=255)
    gcaAGTGACTCAAACCATGAATGCTGGCATCGTTTCCAGCGGCGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAg                                                                                                <  2:664014/100‑1 (MQ=255)
       aGTGACTCAAACCATGAATGCTGGCATCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACg                                                                                             <  1:251762/100‑1 (MQ=255)
       aGTGACTCAAACCATGAATGCTGGCATCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACg                                                                                             <  1:946301/100‑1 (MQ=255)
         tGACTCAAACCATGAATGCTGGCATCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGTCTGAGTCGTa                                                                                           <  1:2322593/100‑1 (MQ=255)
         tGACTCAAACCATGAATGCTGGCATCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTa                                                                                           <  2:1990897/100‑1 (MQ=255)
          gACTCAAACCATGAATGCTGGCATCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTAc                                                                                          >  2:859940/1‑100 (MQ=255)
          gACTCAAACCATGAATGCTGGCATCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGCGTACGACTCAGACGtcc                                                                                          >  2:2420875/1‑98 (MQ=255)
           aCTCAAACCATGAATGCTGGCATCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTAcc                                                                                         >  2:851761/1‑100 (MQ=255)
           aCTCAAACCATGAATGCTGGCATCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTAcc                                                                                         >  1:2374797/1‑100 (MQ=255)
            cTCAAACCATGAATGCTGGCATCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGTCTGAGTCGTAccc                                                                                        <  1:1222835/100‑1 (MQ=255)
            cTCAAACCATGAATGCTGGCATCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTAccc                                                                                        >  2:2107717/1‑100 (MQ=255)
             tCAAACCATGAATGCTGGCATCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGTCTGAGTCGTACCCg                                                                                       <  1:1518854/100‑1 (MQ=255)
             tCAAACCATGAATGCTGGCATCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGTCTGAGTCGTACCCg                                                                                       <  2:2608452/100‑1 (MQ=255)
              cAAGCCATGAATGCTGGCATCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCgg                                                                                      <  2:152508/100‑1 (MQ=255)
              cAAACCATGAATGCTGGCATCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGTCTGAGTCGTACCCgg                                                                                      <  2:2289630/100‑1 (MQ=39)
              cAAACCATGAATGCTGGCATCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGTCTGAGTCGTACCAgg                                                                                      <  2:1134409/100‑1 (MQ=255)
              cAAACCATGAATGCTGGCATCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCgg                                                                                      <  2:2426151/100‑1 (MQ=255)
               aaaCCATGAATGCTGGCATCGTTTCCAGCGGTGAAGTTACATCGACGGGGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGTCTGAGTCGTACCCGGc                                                                                     <  2:1364167/100‑1 (MQ=38)
               aaaCCATGAATGCTGGCATCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGTCTGAGTCGTACCCGGc                                                                                     <  1:965163/100‑1 (MQ=39)
                aaCCATGAATGCTGGCATCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGTCTGAGTCGTACCCgg                                                                                      <  2:658704/98‑1 (MQ=39)
                aaCCATGAATGCTGGCATCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCa                                                                                    >  2:2431845/1‑100 (MQ=255)
                 aCCATGAATGCTGGCATCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCAt                                                                                   <  1:1367609/100‑1 (MQ=255)
                    aTGAATGCTGGCATCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTTGCCGGGTACGTCTGAGTCGTACCCGGcgagat                                                                                <  2:1967847/100‑6 (MQ=37)
                    aTGAATGCTGGCATCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTcc                                                                                <  1:943929/100‑1 (MQ=255)
                    aTGAATGCTGGCATCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTcc                                                                                <  1:894282/100‑1 (MQ=255)
                     tGAATGCTGGCATCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCa                                                                               <  1:856769/100‑1 (MQ=255)
                      gAATGCTGGCATCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCAt                                                                              <  1:1973576/100‑1 (MQ=255)
                      gAATGCTGGCATCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCAt                                                                              >  1:2640022/1‑100 (MQ=255)
                      gAATGCTGGCATCGTTGCCAGCGGTGAAGTTACATCGCCGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTcc                                                                                <  1:893992/98‑1 (MQ=255)
                       aaTGCTGGCATCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATc                                                                             <  1:521806/100‑1 (MQ=255)
                       aaTGCTGGCATCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATc                                                                             <  2:1757931/100‑1 (MQ=255)
                          gCTGGCATCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAAt                                                                          <  2:2519842/100‑1 (MQ=255)
                           cTGGCATCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTTCATCAATa                                                                         >  1:901024/1‑100 (MQ=255)
                           cTGGCATCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATa                                                                         >  2:144186/1‑100 (MQ=255)
                             ggCATCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGa                                                                       <  1:618064/100‑1 (MQ=255)
                             ggCATCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGa                                                                       >  2:1796659/1‑100 (MQ=255)
                               cATCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGAtt                                                                     <  1:2186926/100‑1 (MQ=255)
                               cATCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGAtt                                                                     >  1:738268/1‑100 (MQ=255)
                                aTCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTa                                                                    >  2:1326013/1‑100 (MQ=255)
                                   gTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACaa                                                                 >  1:2338565/1‑100 (MQ=255)
                                    tttCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAAc                                                                <  1:1524677/100‑1 (MQ=255)
                                      tCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGt                                                              <  2:692617/100‑1 (MQ=255)
                                       ccAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTa                                                             >  1:99391/1‑100 (MQ=255)
                                        cAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTaa                                                            >  2:1417004/1‑100 (MQ=255)
                                          gCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAAtt                                                          >  1:1693098/1‑100 (MQ=255)
                                            ggTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGt                                                        >  1:2523650/1‑100 (MQ=255)
                                                aaGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAgc                                                    <  2:204213/100‑1 (MQ=255)
                                                    tACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCa                                                 >  2:605185/1‑99 (MQ=255)
                                                      cATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTc                                              >  1:403996/1‑100 (MQ=255)
                                                       aTCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTcc                                             >  1:91111/1‑100 (MQ=255)
                                                           aCGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCACCAATAGTTTCCACCGTCATTCTCAGCGCAtttc                                             >  2:2177737/1‑94 (MQ=255)
                                                            cGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGa                                        >  2:79538/1‑100 (MQ=255)
                                                              gAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAgg                                      <  1:2431869/100‑1 (MQ=255)
                                                                   ggTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTca                                 >  2:322594/1‑100 (MQ=255)
                                                                   ggTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTca                                 >  2:320169/1‑100 (MQ=255)
                                                                      gCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTcacca                              <  1:2277257/100‑1 (MQ=255)
                                                                        ggTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCaccacc                            >  2:2045654/1‑100 (MQ=255)
                                                                        ggTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCaccacc                            <  2:2526731/100‑1 (MQ=255)
                                                                         gTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCAccacca                           >  2:2661493/1‑100 (MQ=255)
                                                                              cATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGcccc                      <  1:362568/100‑1 (MQ=255)
                                                                              cATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGcccc                      >  1:2517644/1‑100 (MQ=255)
                                                                              cATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGcccc                      <  2:2374773/100‑1 (MQ=255)
                                                                              cATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGcccc                      >  1:1694710/1‑100 (MQ=255)
                                                                               tgatggaatgCCGGGTACGTCTGAGTCGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCt                     >  1:288244/10‑100 (MQ=25)
                                                                               tgatggaatgCCGGGTACGTCTGAGTCGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCt                     >  2:1820915/10‑100 (MQ=37)
                                                                                gatggaatgCCGGGTACGTCTGAGTCGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTg                    >  1:1440447/9‑100 (MQ=37)
                                                                                   aTCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTc                 >  1:159072/1‑100 (MQ=255)
                                                                                    tctcGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAAGCGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCa                <  2:1756020/100‑1 (MQ=255)
                                                                                     ctcGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCAc               <  2:738261/100‑1 (MQ=255)
                                                                                     aatgCCGGGTACGTCTGAGTCGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCAc               >  1:699948/4‑100 (MQ=38)
                                                                                     aatgCCGGGTACGTCTGAGTCGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCAc               >  1:44246/4‑100 (MQ=38)
                                                                                      tcGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACt              <  1:1260963/100‑1 (MQ=255)
                                                                                       tgCCGGGTACGTCTGAGTCGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACtt             >  1:1390045/2‑100 (MQ=38)
                                                                                       cGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACtt             >  1:432375/1‑100 (MQ=255)
                                                                                        gCCGGGTACGTCTGAGTCGTACCCGGCATTCCCTCAATAGATTACAACGTAATTGTCAGCGCCTTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACttt            >  1:577622/1‑100 (MQ=38)
                                                                                        gCCGGGTACGTCTGAGTCGTACCCGGCATTCCATCCCTAGATTACACCGTAATTGTCAGCACATTCCCTTGAGGCTTCACCACAAGCCCCTGCTCAc               >  2:540671/1‑97 (MQ=37)
                                                                                        gCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACttt            <  1:851769/100‑1 (MQ=255)
                                                                                         ccGGGTACGTCTGAGTCGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTc           >  1:238025/1‑100 (MQ=39)
                                                                                         ccGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTc           <  1:2609375/100‑1 (MQ=255)
                                                                                          caggTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCa          <  2:2257008/98‑1 (MQ=255)
                                                                                          cGGGTACGTCTGAGTCGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCa          >  1:1570954/1‑100 (MQ=255)
                                                                                          cGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCa          <  1:2363003/100‑1 (MQ=255)
                                                                                           gggTACGTCTGAGTCGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAg         >  2:1473681/1‑100 (MQ=255)
                                                                                           gggTACGTCTGAGTCGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAg         >  2:1473920/1‑100 (MQ=255)
                                                                                           gggTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAg         <  2:2464955/100‑1 (MQ=255)
                                                                                           gggTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAg         <  1:1605979/100‑1 (MQ=255)
                                                                                            ggTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGc        >  1:2519126/1‑100 (MQ=255)
                                                                                             gTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGcc       <  1:1454641/100‑1 (MQ=255)
                                                                                             gTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGcc       <  2:1050144/100‑1 (MQ=255)
                                                                                             gTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGcc       <  1:859948/100‑1 (MQ=255)
                                                                                                cGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCTCTTTCAGCCTGc    >  1:1400622/1‑100 (MQ=255)
                                                                                                cGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGc    <  1:1594057/100‑1 (MQ=255)
                                                                                                cGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGc    <  2:1091206/100‑1 (MQ=255)
                                                                                                  aCTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGa  <  1:144187/100‑1 (MQ=255)
                                                                                                  aCTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGa  >  1:1832189/1‑100 (MQ=255)
                                                                                                  aCTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGa  >  1:2102364/1‑100 (MQ=255)
                                                                                                  |                                                                                                   
AGCAGCAAGTGACTCAAACCATGAATGCTGGCATCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGA  >  NC_000913/3832090‑3832287

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑