Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,191,997 G→C P213P (CCC→CCG)  thiG ← thiamine biosynthesis ThiGH complex subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,191,9970GC99.1% 417.2 / NA 116P213P (CCC→CCG) thiGthiamine biosynthesis ThiGH complex subunit
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base C (67/48);  minor base A (0/1);  total (67/49)
Fisher's exact test for biased strand distribution p-value = 4.22e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 116 total aligned reads displayed.

GGTGGCATGAGCAAAATAACTGCGGCTGCCCGGTCCGGACTGACGTGCCAGTAGGCCTGCTTCTACCGCCAGACGAAATGCCTTCGCCATGTTGACGGGATCGTCCGCGACGGCAATCGCCGTATTCACTAACACCGCGTCGGCCCCCATTTCCAGCGCCTGCGCGGCATGGCTGGGAACGCCGATGCCAGCA  >  NC_000913/4191901‑4192093
                                                                                                |                                                                                                
ggtggCATGAGCAAAATAACTGCGGCTGCCCGGTCCGGACTGACGTGCCAGTAGGCCTGCTTCTACCGCCAGACGAAATGCCTTCGCCATGTTGACCGGa                                                                                               >  1:2609696/1‑100 (MQ=255)
 gtggCATGAGCAAAATAACTGCGGCTGCCCGGTCCGGACTGACGTGCCAGTAGGCCTGCTTCTACCGCCAGACGAAATGCCTTCGCCATGTTGACCGGAt                                                                                              <  1:2133097/100‑1 (MQ=255)
 gtggCATGAGCAAAATAACTGCGGCTGCCCGGTCCGGACTGACGTGCCAGTAGGCCTGCTTCTACCGCCAGACGAAATGCCTTCGCCATGTTGACCGGAt                                                                                              <  1:896570/100‑1 (MQ=255)
   ggCATGAGCAAAATAACTGCGGCTGCCCGGTCCGGACTGACGTGCCAGTAGGCCTGCTTCTACCGCCAGACGAAATGCCTTCGCCATGTTGACCGGATCg                                                                                            <  1:1575333/100‑1 (MQ=255)
   ggCATGAGCAAAATAACTGCGGCTGCCCGGTCCGGACTGACGTGCCAGTAGGCCTGCTTCTACCGCCAGACGAAATGCCTTCGCCATGTTGACCGGATCg                                                                                            >  2:187979/1‑100 (MQ=255)
     cATGAGCAAAATAACTGCGGCTGCCCGGTCCGGACTGACGTGCCAGTAGGCCTGCTTCTACCGCCAGACGAAATGCCTTCGCCATGTTGACCGGATCGTc                                                                                          <  2:304129/100‑1 (MQ=255)
      aTGAGCAAAATAACTGCGGCTGCCCGGTCCGGACTGACGTGCCAGTAGGCCTGCTTCTACCGCCAGACGAAATGCCTTCGCCATGTTGACCGGATCGTcc                                                                                         <  1:1033685/100‑1 (MQ=255)
       tGAGCAAAATAACTGCGGCTGCCCGGTCCGGACTGACGTGCCAGTAGGCCTGCTTCTACCGCCAGACGAAATGCCTTCGCCATGTTGACCGGATCGTCcg                                                                                        <  1:1180904/100‑1 (MQ=255)
        gAGCAAAATAACTGCGGCTGCCCGGTCCGGACTGACGTGCCAGTAGGCCTGCTTCTACCGCCAGACGAAATGCCTTCGCCATGTTGACCGGATCGTCcgc                                                                                       >  2:881129/1‑100 (MQ=255)
        gAGCAAAATAACTGCGGCTGCCCGGTCCGGACTGACGTGCCAGTAGGCCTGCTTCTACCGCCAGACGAAATGCCTTCGCCATGTTGACCGGATCGTCcgc                                                                                       >  2:1098605/1‑100 (MQ=255)
         aGCAAAATAACTGCGGCTGCCCGGTCCGGACTGACGTGCCAGTAGGCCTGCTTCTACCGCCAGACGAAATGCCTTCGCCATGTTGACCGGATCGTCcgcg                                                                                      <  1:258045/100‑1 (MQ=255)
           cAAAATAACTGCGGCTGCCCGGTCCGGACTGACGTGCCAGTAGGCCTGCTTCTACCGCCAGACGAAATGCCTTCGCCATGTTGACCGGATCGTCCGCGAc                                                                                    >  2:2296971/1‑100 (MQ=255)
             aaaTAACTGCGGCTGCCCGGTCCGGACTGACGTGCCAGTAGGCCTGCTTCTACCGCCAGACGAAATGCCTTCGCCATGTTGACCGGATCGTCCGCGACgg                                                                                  <  1:866045/100‑1 (MQ=255)
              aaTAACTGCGGCTGCCCGGTCCGGACTGACGTGCCAGTAGGCCTGCTTCTACCGCCAGACGAAATGCCTTCGCCATGTTGACCGGATCGTCCGCGACGGc                                                                                 <  2:2224470/100‑1 (MQ=255)
              aaTAACTGCGGCTGCCCGGTCCGGACTGACGTGCCAGTAGGCCTGCTTCTACCGCCAGACGAAATGCCTTCGCCATGTTGACCGGATCGTCCGCGACGGc                                                                                 >  2:1737574/1‑100 (MQ=255)
                 aaCTGCGGCTGCCCGGTCCGGACTGACGTGCCAGTAGGCCTGCTTCTACCGCCAGACGAAATGCCTTCGCCATGTTGACCGGATCGTCCGCGACGGCAAt                                                                              >  2:1002224/1‑100 (MQ=255)
                  ncTGCGGCTGCCCGGTCCGGACTGACGTGCCAGTAGGCCTGCTTCTACCGCCAGACGAAATGCCTTCGCCATGTTGACCGGATCGTCCGCGACGGCAATc                                                                             >  2:599977/2‑100 (MQ=255)
                  aCTGCGGCTGCCCGGTCCGGACTGACGGGCCAGTAGGCCTGCTTCTACCGCCAGACGAAATGCCTTCGCCATGTTGACCGGATCGTCCGCGACGGc                                                                                 <  2:520083/96‑1 (MQ=255)
                    tGCGGCTGCCCGGTCCGGACTGACGTGCCAGTAGGCCTGCTTCTACCGCCAGACGAAATGCCTTCGCCATGTTGACCGGATCGTCCGCGACGGCAATCg                                                                            >  2:2022117/1‑99 (MQ=255)
                    tGCGGCTGCCCGGTCCGGACTGACGTGCCAGTAGGCCTGCTTCTACCGCCAGACGAAATGCCTTCGCCATGTTGACCGGATCGTCCGCGACGGCAATCGc                                                                           >  1:2608471/1‑100 (MQ=255)
                    tGCGGCTGCCCGGTCCGGACTGACGTGCCAGTAGGCCTGCTTCTACCGCCAGACGAAATGCCTTCGCCATGTTGACCGGATCGTCCGCGACGGCAATCGc                                                                           >  1:1778301/1‑100 (MQ=255)
                     gCGGCTGCCCGGTCCGGACTGACGTGCCAGTAGGCCTGCTTCTACCGCCAGACGAAATGCCTTCGCCATGTTGACCGGATCGTCCGCGACGGCAATCGcc                                                                          >  1:1183854/1‑100 (MQ=255)
                      cGGCTGCCCGGTCCGGACTGACGTGCCAGTAGGCCTGCTTCTACCGCCAGACGAAATGCCTTCGCCATGTTGACCGGATCGTCCGCGACGGCAATCGCCg                                                                         <  1:2177182/100‑1 (MQ=255)
                      cGGCTGCCCGGTCCGGACTGACGTGCCAGTAGGCCTGCTTCTACCGCCAGACGAAATGCCTTCGCCATGTTGACCGGATCGTCCGAGACGGCAATCGCCg                                                                         <  2:1316121/100‑1 (MQ=255)
                       gggtGCCCGGTCCGGAATGACGTGCCAGTAGGCCTGCTCCTACCGCCAGACGAAATGCCTTCGCCATGTTGACCGGATCGTCCGCGACGGCAATCGCCg                                                                         <  2:892839/96‑1 (MQ=255)
                               ggTCCGGACTGACGTGCCAGTAGGCCTGCTTCTACCGCCAGACGAAATGCCTTCGCCATGTTGACCGGATCGTCCGCGACGGCAATCGCCGTATTCACTa                                                                >  1:1212974/1‑100 (MQ=255)
                               ggTCCGGACTGACGTGCCAGTAGGCCTGCTTCTACCGCCAGACGAAATGCCTTCGCCATGTTGACCGGATCGTCCGCGACGGCAATCGCCGTATTCACTa                                                                <  1:2388604/100‑1 (MQ=255)
                                   cGGACTGACGTGCCAGTAGGCCTGCTTCTACCGCCAGACGAAATGCCTTCGCCATGTTGACCGGATCGTCCGCGACGGCAATCGCCGTATTCACTAacac                                                            <  1:1546050/100‑1 (MQ=255)
                                     gACTGACGTGCCAGTAGGCCTGCTTCTACCGCCAGACGAAATGCCTTCGCCATGTTGACCGGATCGTCCGCGACGGCAATCGCCGTATTCACTAACACcg                                                          <  2:1211744/100‑1 (MQ=255)
                                     gACTGACGTGCCAGTAGGCCTGCTTCTACCGCCAGACGAAATGCCTTCGCCATGTTGACCGGATCGTCCGCGACGGCAATCGCCGTATTCACTAACACcg                                                          >  1:2475703/1‑100 (MQ=255)
                                       cTGACGTGCCAGTAGGCCTGCTTCTACCGCCAGACGAAATGCCTTCGCCATGTTGACCGGATCGTCCGCGACGGCAATCGCCGTATTCACTAACACcgcg                                                        >  1:385501/1‑100 (MQ=255)
                                        tGACGTGCCAGTAGGCCTGCTTCTACCGCCAGACGAAATGCCTTCGCCATGTTGACCGGATCGTCCGCGACGGCAATCGCCGTATTCACTAACACCGCGt                                                       >  2:2548025/1‑100 (MQ=255)
                                         gACGTGCCAGTAGGCCTGCTTCTACCGCCAGACGAAATGCCTTCGCCATGTTGACCGGATCGTCCGCGACGGCAATCGCCGTATTCACTAACACCGCGTc                                                      <  1:1050278/100‑1 (MQ=255)
                                          aCGTGCCAGTAGGCCTGCTTCTACCGCCAGACGAAATGCCTTCGCCATGTTGACCGGATCGTCCGCGACGGCAATCGCCGTATTCACTAACACCGCGTCg                                                     >  2:964667/1‑100 (MQ=255)
                                          aCGTGCCAGTAGGCCTGCTTCTACCGCCAGACGAAATGCCTTCGCCATGTTGACCGGATCGTCCGCGACGGCAATCGCCGTATTCACTAACACCGCGTCg                                                     >  2:2511555/1‑100 (MQ=255)
                                           cGTGCCAGTAGGCCTGCTTCTACCGCCAGACGAAATGCCTTCGCCATGTTGACCGGATCGTCCGCGACGGCAATCGCCGTATTAACTAACACCGCGTCgg                                                    >  2:1579435/1‑100 (MQ=255)
                                           cGTGCCAGTAGGCCTGCTTCTACCGCCAGACGAAATGCCTTCGCCATGTTGACCGGATCGTCCGCGACGGCAATCGCCGTATTAACTAACACCGCGTCgg                                                    >  2:1576031/1‑100 (MQ=255)
                                            gTGCCAGTAGGCCTGCTTCTACCGCCAGACGAAATGCCTTCGCCATGTTGACCGGATCGTCCGCGACGGCAATCGCCGTATTCACTAACACCGCGTCGGc                                                   <  1:1343854/100‑1 (MQ=255)
                                             tGCCAGTAGGCCTGCTTCTACCGCCAGACGAAATGCCTTCGCCATGTTGACCGGATCGTCCGCGACGGCAATCGCCGTATTCACTAACACCGCGTCGGcc                                                  >  2:2314914/1‑100 (MQ=255)
                                             tGCCAGTAGGCCTGCTTCTACCGCCAGACGAAATGCCTTCGCCATGTTGACCGGATCGTCCGCGACGGCAATCGCCGTATTCACTAACACCGCGTCGGcc                                                  >  1:1267917/1‑100 (MQ=255)
                                             tGCCAGTAGGCCTGCTTCTACCGCCAGACGAAATGCCTTCGCCATGTTGACCGGATCGTCCGCGACGGCAATCGCCGTATTCACTAACACCGCGTCGGcc                                                  >  1:185560/1‑100 (MQ=255)
                                             tGCCAGTAGGCCTGCTTCTACCGCCAGACGAAATGCCTTCGCCATGTTGACCGGATCGTCCGCGACGGCAATCGCCGTATTCACTAACACCGCGTCGGcc                                                  >  1:2661701/1‑100 (MQ=255)
                                              gCCAGTAGGCCTGCTTCTACCGCCAGACGAAATGCCTTCGCCATGTTGACCGGATCGTCCGCGACGGCAATCGCCGTATTCACTAACACCGCGTCGGccc                                                 >  1:2196211/1‑100 (MQ=255)
                                              gCCAGTAGGCCTGCTTCTACCGCCAGACGAAATGCCTTCGCCATGTTGACCGGATCGTCCGCGACGGCAATCGCCGTATTCACTAACACCGCGTCGGccc                                                 >  2:2266641/1‑100 (MQ=255)
                                               ccAGTAGGCCTGCTTCTACCGCCAGACGAAATGCCTTCGCCATGTTGACCGGATCGTCCGCGACGGCAATCGCCGTATTCACTAACACCGCGTCGGcccc                                                <  1:1936821/100‑1 (MQ=255)
                                                     ggCCTGCTTCTACCGCCAGACGAAATGCCTTCGCCATGTTGACCGGATCGTCCGCGACGGCAATCGCCGTATTCACTAACACCGCGTCGGCCCCCATTTc                                          <  1:2603846/100‑1 (MQ=255)
                                                     ggCCTGCTTCTACCGCCAGACGAAATGCCTTCGCCATGTTGACCGGATCGTCCGCGACGGCAATCGCCGTATTCACTAACACCGCGTCGGCCCCCATTTc                                          >  1:2145236/1‑100 (MQ=255)
                                                        cTGCTTCTACCGCCAGACGAAATGCCTTCGCCATGTTGACCGGATCGTCCGCGACGGCAATCGCCGTATTCACTAACACCGCGTCGGCCCCCATTTCCAg                                       <  2:493638/100‑1 (MQ=255)
                                                        cTGCTTCTACCGCCAGACGAAATGCCTTCGCCATGTTGACCGGATCGTCCGCGACGGCAATCGCCGTATTCACTAACACCGCGTCGGCCCCCATTTCCAg                                       <  1:1927144/100‑1 (MQ=255)
                                                          gCTTCTACCGCCAGACGAAATGCCTTCGCCATGTTGACCGGATCGTCCGCGACGGCAATCGCCGTATTCACTAACACCGCGTCGGCCCCCATTTCCAgcg                                     >  1:2624661/1‑100 (MQ=255)
                                                          gCTTCTACCGCCAGACGAAATGCCTTCGCCATGTTGACCGGATCGTCCGCGACGGCAATCGCCGTATTCACTAACACCGCGTCGGCCCCCATTTCCAgcg                                     >  2:791717/1‑100 (MQ=255)
                                                           cTTCTACCGCCAGACGAAATGCCTTCGCCATGTTGACCGGATCGTCCGCGACGGCAATCGCCGTATTCACTAACACCGCGTCGGCCCCCATTTCCAgcgc                                    >  1:1500391/1‑100 (MQ=255)
                                                            ttCTACCGCCAGACGAAATGCCTTCGCCATGTTGACCGGATCGTCCGCGACGGCAATCGCCGTATTCACTAACCCCGCGTCGGCCCCCATTTCCAGCGcc                                   >  1:2572567/1‑100 (MQ=255)
                                                            ttCTACCGCCAGACGAAATGCCTTCGCCATGTTGACCGGATCGTCCGCGACGGCAATCGCCGTATTCACTAACACCGCGTCGGCCCCCATTTCCAGCGcc                                   >  2:2403880/1‑100 (MQ=255)
                                                            ttCTACCGCCAGACGAAATGCCTTCGCCATGTTGACCGGATCGTCCGCGACGGCAATCGCCGTATTCACTAACACCGCGTCGGCCCCCATTTCCAGCGcc                                   <  1:1032520/100‑1 (MQ=255)
                                                            ttCTACCGCCAGACGAAATGCCTTCGCCATGTTGACCGGATCGTCCGCGACGGCAATCGCCGTATTCACTAACACCGCGTCGGCCCCCATTTCCAGCGcc                                   <  1:1724630/100‑1 (MQ=255)
                                                                aCCGCCAGACGAAATGCCTTCGCCATGTTGACCGGATCGTCCGCGACGGCAATCGCCGTATTCACTAACACCGCGTCGGCCCCCATTTCCAGCGCCTgc                                >  2:1353910/1‑99 (MQ=255)
                                                                  cGCCAGACGAAATGCCTTCGCCATGTTGACCGGATCGTCCGCGACGGCAATCGCCGTATTCACTAACACCGCGTCGGCCCCCATTTCCAGCGCCTGcgcg                             >  1:1672391/1‑100 (MQ=255)
                                                                  cGCCAGACGAAATGCCTTCGCCATGTTGACCGGATCGTCCGCGACGGCAATCGCCGTATTCACTAACACCGCGTCGGCCCCCATTTCCAGCGCCTGcgcg                             >  2:2201020/1‑100 (MQ=255)
                                                                  cGCCAGACGAAATGCCTTCGCCATGTTGACCGGATCGTCCGCGACGGCAATCGCCGTATTCACTAACACCGCGTCGGCCCCCATTTCCAGCGCCTGcgcg                             >  2:1530545/1‑100 (MQ=255)
                                                                  cGCCAGACGAAATGCCTTCGCCATGTTGACCGGATCGTCCGCGACGGCAATCGCCGTATTCACTAACACCGCGTCGGCCCCCATTTCCAGCGCCTGcgcg                             >  2:445233/1‑100 (MQ=255)
                                                                   gCCAGACGAAATGCCTTCGCCATGTTGACCGGATCGTCCGCGACGGCAATCGCCGTATTCACTAACACCGCGTCGGCCCCCATTTCCAGCGCCTGCGCgg                            <  1:811195/100‑1 (MQ=255)
                                                                   gCCAGACGAAATGCCTTCGCCATGTTGACCGGATCGTCCGCGACGGCAATCGCCGTATTCACTAACACCGCGTCGGCCCCCATTTCCAGCGCCTGCGCgg                            <  1:2683277/100‑1 (MQ=255)
                                                                    ccAGACGAAATGCCTTCGCCATGTTGACCGGATCGTCCGCGACGGCAATCGCCGTATTCACTAACACCGCGTCGGCCCCCATTTCCAGCGCCTGCGCGGc                           <  2:1222421/100‑1 (MQ=255)
                                                                     cAGACGAAATGCCTTCGCCATGTTGACCGGATCGTCCGCGACGGCAATCGCCGTATTCACTAACACCGCGTCGGCCCCCATTTCCAGCGCCTGCGCgg                            >  2:2528246/1‑98 (MQ=255)
                                                                     cAGACGAAATGCCTTCGCCATGTTGACCGGATCGTCCGCGACGGCAATCGCCGTATTCACTAACACCGCGTCGGCCCCCATTTCCAGCGCCTGCGCGGCa                          <  1:645695/100‑1 (MQ=255)
                                                                      aGACGAACTGACTTCGCCATGTTGACAGCATCGTCCGCGACGCCAATCGACGAACCCACTAACACCGCGTCGGCCCCCATTTCCAGCGCCTGCGCGGCa                          <  2:221808/99‑1 (MQ=255)
                                                                      aGACGAAATGCCTTCGCCATGTTGACCGGATCGTCCGCGACGGCAATCGCCGTATTCACTAACACCGCGTCGGCCCCCATTTCCAGCGCCTGCGCGGCAt                         >  1:1602808/1‑100 (MQ=255)
                                                                      aGACGAAATGCCTTCGCCATGTTGACCGGATCGTCCGCGACGGCAATCGCCGTATTCACTAACACCGCGTCGGCCCCCATTTCCAGCGCCTGCGCGGCAt                         >  1:2188734/1‑100 (MQ=255)
                                                                       gACGAAATGCCTTCGCCATGTTGACCGGATCGTCCGCGACGGCAATCGCCGTATTCACTAACACCGCGTCGGCCCCCATTTCCAGCGCCTGCGCGGCAt                         <  1:2524674/99‑1 (MQ=255)
                                                                         cGAAATGCCTTCGCCATGTTGACCGGATCGTCCGCGACGGCAATCGCCGTATTCACTAACACCGCGTCGGCCCCCATTTCCAGCGCCTGCGCGGCATGGc                      >  2:740000/1‑100 (MQ=255)
                                                                         cGAAATGCCTTCGCCATGTTGACCGGATCGTCCGCGACGGCAATCGCCGTATTCACTAACACCGCGTCGGCCCCCATTTCCAGCGCCTGCGCGGCATGGc                      >  1:1605590/1‑100 (MQ=255)
                                                                         cGAAATGCCTTCGCCATGTTGACCGGATCGTCCGCGACGGCAATCGCCGTATTCACTAACACCGCGTCGGCCCCCATTTCCAGCGCCTGCGCGGCATGGc                      >  1:2224324/1‑100 (MQ=255)
                                                                         cGAAATGCCTTCGCCATGTTGACCGGATCGTCCGCGACGGCAATCGCCGTATTCACTAACACCGCGTCGGCCCCCATTTCCAGCGCCTGCGCGGCATGGc                      >  1:2370193/1‑100 (MQ=255)
                                                                         cGAAATGCCTTCGCCATGTTGACCGGATCGTCCGCGACGGCAATCGCCGTATTCACTAACACCGCGTCGGCCCCCATTTCCAGCGCCTGCGCGGCATGGc                      <  1:333163/100‑1 (MQ=255)
                                                                           aaaTGCCTTCGCCATGTTGACCGGATCGTCCGCGACGGCAATCGCCGTATTCACTAACACCGCGTCGGCCCCCATTTCCAGCGCCTGCGCGGCATGGCTg                    <  1:903119/100‑1 (MQ=255)
                                                                           aaaTGCCTTCGCCATGTTGACCGGATCGTCCGCGACGGCAATCGCCGTATTCACTAACACCGCGTCGGCCCCCATTTCCAGCGCCTGCGCGGCATGGCTg                    >  1:351953/1‑100 (MQ=255)
                                                                            aaTGCCTTCGCCATGTTGACCGGATCGTCCGCGACGGCAATCGCCGTATTCACTAACACCGCGTCGGCCCCCATTTCCAGCGCCTGCGCGGCATGGCTgg                   <  2:1183845/100‑1 (MQ=255)
                                                                            aaTGCCTTCGCCATGTTGACCGGATCGTCCGCGACGGCAATCGCCGTATTCACTAACACCGCGTCGGCCCCCATTTCCAGCGCCTGCGCGGCATGGCTgg                   <  1:2011383/100‑1 (MQ=255)
                                                                             aTGCCTTCGCCATGTTGACCGGATCGTCCGCGACGGCAATCGCCGTATTCACTAACACCGCGTCGGCCCCCATTTCCAGCGCCTGCGCGGCATGGCTggg                  <  1:2314938/100‑1 (MQ=255)
                                                                             aTGCCTTCGCCATGTTGACCGGATCGTCCGCGACGGCAATCGCCGTATTCACTAACACCGCGTCGGCCCCCATTTCCAGCGCCTGCGCGGCATGGCTggg                  <  1:2054542/100‑1 (MQ=255)
                                                                             aTGCCTTCGCCATGTTGACCGGATCGTCCGCGACGGCAATCGCCGTATTCACTAACACCGCGTCGGCCCCCATTTCCAGCGCCTGCGCGGCATGGCTggg                  <  2:2145214/100‑1 (MQ=255)
                                                                              tGCCTTCGCCATGTTGACCGGATCGTCCGCGACGGCAATCGCCGTATTCACTAACACCGCGTCGGCCCCCATTTCCAGCGCCTGCGCGGCATGGCTGGGa                 >  1:2001823/1‑100 (MQ=255)
                                                                               gCCTTCGCCATGTTGACCGGATCGTCCGCGACGGCAATCGCCGTATTCACTAACACCGCGTCGGCCCCCATTTCCAGCGCCTGCGCGGCATGGCTGGGaa                >  2:2134900/1‑100 (MQ=255)
                                                                                 cTTCGCCATGTTGACCGGATCGTCCGCGACGGCAATCGCCGTATTCACTAACACCGCGTCGGCCCCCATTTCCAGCGCCTGCGCGGCATGGCTGGGAACg              <  2:2195125/100‑1 (MQ=255)
                                                                                 cTTCGCCATGTTGACCGGATCGTCCGCGACGGCAATCGCCGTATTCACTAACACCGCGTCGGCCCCCATTTCCAGCGCCTGCGCGGCATGGCTGGGAACg              >  1:1957201/1‑100 (MQ=255)
                                                                                 cTTCGCCATGTTGACCGGATCGTCCGCGACGGCAATCGCCGTATTCACTAACACAGCGTCGGCCCCCATTTACAGCGCCTGCGCGGCATGGCTGGGAACg              >  2:2155581/1‑100 (MQ=255)
                                                                                     gCCATGTTGACCGGATCGTCCGCGACGGCAATCGCCGTATTCACTAACACCGCGTCGGCCCCCATTTCCAGCGCCTGCGCGGCATGGCTGGGAACGCCGa          >  1:1036864/1‑100 (MQ=255)
                                                                                     gCCATGTTGACCGGATCGTCCGCGACGGCAATCGCCGTATTCACTAACACCGCGTCGGCCCCCATTTCCAGCGCCTGCGCGGCATGGCTGGGAACGCCGa          >  2:488465/1‑100 (MQ=255)
                                                                                     gCCATGTTGACCGGATCGTCCGCGACGGCAATCGCCGTATTCACTAACACCGCGTCGGCCCCCATTTCAAGCGCCTGCGCGGCATGGCTGGGAACGCCGa          <  2:861288/100‑1 (MQ=255)
                                                                                      ccATGTTGACCGGATCGTCCGCGACGGCAATCGCCGTATTCACTAACACCGCGTCGGCCCCCATTTCCAGCGCCTGCGCGGCATGGCTGGGAACGCCGAt         <  1:1013276/100‑1 (MQ=255)
                                                                                       cATGTTGACCGGATCGTCCGCGACGGCAATCGCCGTATTCACTAACACCGCGTCGGCCCCCATTTCCAGCGCCTGCGCGGCATGGCTGGGAACGCCGATg        >  1:1023239/1‑100 (MQ=255)
                                                                                        aTGTTGACCGGATCGTCCGCGACGGCAATCGCCGTATTCACTAACACCGCGTCGGCCCCCATTTCCAGCGCCTGCGCGGCATGGCTGGGAACGCCGATGc       <  1:1002232/100‑1 (MQ=255)
                                                                                         tGTTGACCGGATCGTCCGCGACGGCAATCGCCGTATTCACTAACACCGCGTCGGCCCCCATTTCCAGCGCCTGCGCGGCATGGCTGGGAACGCCGATg        <  2:2243328/98‑1 (MQ=255)
                                                                                         tGTTGACCGGATCGTCCGCGACGGCAATCGCCGTATTCACTAACACCGCGTCGGCCCCCATTTCCAGCGCCTGCGCGGCATGGCTGGGAACGCCGATGcc      <  1:787932/100‑1 (MQ=255)
                                                                                         tGTTGACCGGATCGTCCGCGACGGCAATCGCCGTATTCACTAACACCGCGTCGGCCCCCATTTCCAGCGCCTGCGCGGCATGGCTGGGAACGCCGATGcc      >  2:1604552/1‑100 (MQ=255)
                                                                                           ttGACCGGATCGTCCGCGACGGCAATCGCCGTATTCACTAACACCGCGTCGGCCCCCATTTCCAGCGCCTGCGCGGCATGGCTGGGAACGCCGATGCCAg    <  2:385496/100‑1 (MQ=255)
                                                                                           ttGACCGGATCGTCCGCGACGGCAATCGCCGTATTCACTAACACCGCGTCGGCCCCCATTTCCAGCGCCTGCGCGGCATGGCTGGGAACGCCGATGCCAg    <  1:1098614/100‑1 (MQ=255)
                                                                                             gACCGGATCGTCCGCGACGGCAATCGCCGTATTCACTAACACCGCGTCGGCCCCCATTTCCAGCGCCTGCGCGGCATGGCTGGGAACGCCGATGCCAg    <  2:2608443/98‑1 (MQ=255)
                                                                                             gACCGGATCGTCCGCGACGGCAATCGCCGTATTCACTAACACCGCGTCGGCCCCCATTTCCAGCGCCTGCGCGGCATGGCTGGGAACGCCGATGCCAGCa  >  2:516579/1‑100 (MQ=255)
                                                                                                |                                                                                                
GGTGGCATGAGCAAAATAACTGCGGCTGCCCGGTCCGGACTGACGTGCCAGTAGGCCTGCTTCTACCGCCAGACGAAATGCCTTCGCCATGTTGACGGGATCGTCCGCGACGGCAATCGCCGTATTCACTAACACCGCGTCGGCCCCCATTTCCAGCGCCTGCGCGGCATGGCTGGGAACGCCGATGCCAGCA  >  NC_000913/4191901‑4192093

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑