BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,754,639	C→A	Q42H (CAG→CAT)	ratA ←	toxic UPF0083 family protein inhibitor of 70S ribosome formation

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,754,639	0	C	A	100.0%	361.6 / NA	104	Q42H (CAG→CAT)	ratA	toxic UPF0083 family protein inhibitor of 70S ribosome formation
Reads supporting (aligned to +/- strand): ref base C (0/0); new base A (57/47); total (57/47)

Only 100 of 106 total aligned reads displayed.

CGTTTTGCTGATCCCAGCCTTAGAGACATCTACCGCCGCAGTCATCTGCCCAGGAGTGGACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAACTGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCTCCGCGCTGTAGGGTACCAGTGCGGTCCGGCTAATCTGAGGCATAACAATTT  >  NC_000913/2754543‑2754733

cGTTTTGCTGATCCCAGCCTTAGAGACATCTACCGCCGCAGTCATCTGCCCAGGAGTGGACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGa                                                                                             >  1:339665/1‑100 (MQ=255)

 gTTTTGCTGATCCCAGCCTTAGAGACATCTACCGCCGCAGTCATCTGCCCAGGAGTGGACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAg                                                                                            <  2:1450561/100‑1 (MQ=255)

   tttGCTGATCCCAGCCTTAGAGACATCTACCGCCGCAGTCATCTGCCCAGGAGTGGACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGa                                                                                          <  1:506274/100‑1 (MQ=255)

   tttGCTGATCCCAGCCTTAGAGACATCTACCGCCGCAGTCATCTGCCCAGGAGTGGACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGa                                                                                          >  2:2336785/1‑100 (MQ=255)

    ttGCTGATCCCAGCCTTAGAGACATCTACCGCCGCAGTCATCTGCCCAGGAGTGGACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGAt                                                                                         >  1:2373751/1‑100 (MQ=255)

     tGCTGATCCCAGCCTTAGAGACATCTACCGCCGCAGTCATCTGCCCAGGAGTGGACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATa                                                                                        <  1:1246652/100‑1 (MQ=255)

      gCTGATCCCAGCCTTAGAGACATCTACCGCCGCAGTCATCTGCCCAGGAGTGGACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATa                                                                                        >  1:46418/1‑99 (MQ=255)

        tGATCCCAGCCTTAGAGACATCTACCGCCGCAGTCATCTGCCCAGGAGTGGACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGa                                                                                     >  2:1483520/1‑100 (MQ=255)

         gATCCCAGCCTTAGAGACATCTACCGCCGCAGTCATCTGCCCAGGAGTGGACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGAc                                                                                    >  2:2159752/1‑100 (MQ=255)

         gATCCCAGCCTTAGAGACATCTACCGCCGCAGTCATCTGCCCAGGAGTGGACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGAc                                                                                    >  1:1379126/1‑100 (MQ=255)

          aTCCCAGCCTTAGAGACATCTACCGCCGCAGTCATCTGCCCAGGAGTGGACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACt                                                                                   >  2:1860469/1‑100 (MQ=255)

            cccAGCCTTAGAGACATCTACCGCCGCAGTCATCTGCCCAGGCGTGGACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGa                                                                                 >  2:1757238/1‑100 (MQ=255)

             ccAGCCTTAGAGACATCTACCGCCGCAGTCATCTGCCCAGGAGTGGACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGaa                                                                                <  1:491529/100‑1 (MQ=255)

                gCCTTAGAGACATCTACCGCCGCAGTCATCTGCCCAGGAGTGGACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGt                                                                             <  1:754955/100‑1 (MQ=255)

                gCCTTAGAGACATCTACCGCCGCAGTCATCTGCCCAGGAGTGGACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGt                                                                             >  1:708756/1‑100 (MQ=255)

                 ccTTAGAGACATCTACCGCCGCAGTCATCTGCCCAGGAGTGGACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTc                                                                            >  1:331647/1‑100 (MQ=255)

                  cTTAGAGACATCTACCGCCGCAGTCATCTGCCCAGGAGTGGACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCa                                                                           >  1:53354/1‑100 (MQ=255)

                   ttAGAGACATCTACCGCCGCAGTCATCTGCCCAGGAGTGGACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCAt                                                                          >  2:964926/1‑100 (MQ=255)

                    tAGAGACATCTACCGCCGCAGTCATCTGCCCAGGAGTGGACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCAtt                                                                         >  1:702955/1‑100 (MQ=255)

                     agagACATCTACCGCCGCAGTCATCTGCCCAGGAGTGGACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTc                                                                        <  1:757749/100‑1 (MQ=255)

                      gagaCATCTACCGCCGCAGTCATCTGCCCAGGAGTGGACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCa                                                                       <  1:2443676/100‑1 (MQ=255)

                      gagaCATCTACCGCCGCAGTCATCTGCCCAGGAGTGGACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCa                                                                       >  1:1201741/1‑100 (MQ=255)

                       agaCATCTACCGCCGCAGTCATCTGCCCAGGAGTGGACTCCAGAAGCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCAc                                                                      <  2:661794/100‑1 (MQ=255)

                        gaCATCTACCGCCGCAGTCATCTGCCCAGGAGTGGACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACt                                                                     <  2:744027/100‑1 (MQ=255)

                        gaCATCTACCGCCGCAGTCATCTGCCCAGGAGTGGACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACt                                                                     >  1:1321060/1‑100 (MQ=255)

                        gaCATCTACCGCCGCAGTCATCTGCCCAGGAGTGGACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACt                                                                     >  2:1045156/1‑100 (MQ=255)

                         aCATCTACCGCCGCAGTCATCTGCCCAGGAGTGGACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTa                                                                    <  1:2336809/100‑1 (MQ=255)

                          cATCTACCGCCGCAGTCATCTGCCCAGGAGTGGACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTaa                                                                   >  1:1619415/1‑100 (MQ=255)

                           aTCTACCGCCGCAGTCATCTGCCCAGGAGTGGACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAAc                                                                  <  1:2523078/100‑1 (MQ=255)

                            tCTACCGCCGCAGTCATCTGCCCAGGAGTGGACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACt                                                                 <  1:2612536/100‑1 (MQ=255)

                               aCCGCCGCAGTCATCTGCCCAGGAGTGGACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGAt                                                              >  2:1175433/1‑100 (MQ=255)

                               aCCGCCGCAGTCATCTGCCCAGGAGTGGACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGAt                                                              <  2:606416/100‑1 (MQ=255)

                               aCCGCCGCAGTCATCTGCCCAGGAGTGGACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGAt                                                              >  2:679350/1‑100 (MQ=255)

                               aCCGCCGCAGTCATCTGCCCAGGAGTGGACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGAt                                                              >  2:711478/1‑100 (MQ=255)

                                ccgccgCAGTCATCTGCCCAGGAGTGGACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATa                                                             <  1:1426243/100‑1 (MQ=255)

                                ccgccgCAGTCATCTGCCCAGGAGTGGACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGACATTCACTAACTGATa                                                             >  2:889317/1‑100 (MQ=255)

                                ccgccgCAGTCATCTGCCCAGGAGTGGACTCCAGAATCCGACTTCCGGTAAAAAAAAAAAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATa                                                             <  2:2539761/100‑1 (MQ=255)

                                  gccgcAGTCATCTGCCCAGGAGTGGACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACa                                                           <  1:1606592/100‑1 (MQ=255)

                                  gccgcAGTCATCTGCCCAGGAGTGGACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACa                                                           >  1:958064/1‑100 (MQ=255)

                                    cgcAGTCATCTGCCCAGGAGTGGACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACAtt                                                         >  2:2117217/1‑100 (MQ=255)

                                     gcAGTCATCTGCCCAGGAGTGGACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACAttt                                                        <  2:562/100‑1 (MQ=255)

                                     gcAGTCATCTGCCCAGGAGTGGACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACAttt                                                        >  1:872269/1‑100 (MQ=255)

                                       aGTCATCTGCCCAGGAGTAGACTCCAGAATCCGACTTCCGGAAAAAAAAAAAAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGc                                                      <  2:2093299/100‑1 (MQ=255)

                                        gTCATCTGCCCAGGAGTGGACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCt                                                     >  2:676893/1‑100 (MQ=255)

                                        gTCATCTGCCCAGGAGTGGACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCt                                                     >  1:1198498/1‑100 (MQ=255)

                                        gTCATCTGCCCAGGAGTGGACTCCAGAATCCGACTTCCGGTAAAAAAAAAAAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCt                                                     <  2:1379114/100‑1 (MQ=255)

                                          caactacccAGGAGTGGACACCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGACATTCACTAACTGATACATTTGc                                                      >  2:467605/7‑97 (MQ=255)

                                              tGCCCAGGAGTGGACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCTCCgcgc                                               <  1:964934/100‑1 (MQ=255)

                                                  cAGGAGTGGACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCTCCGCGCTGTa                                           >  2:1535669/1‑100 (MQ=255)

                                                    ggAGTGGACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCTCCGCGCTGTAgg                                         <  2:958056/100‑1 (MQ=255)

                                                     gAGTGGACTCCAGAATCCGACTTCCTGTACAACCCGGCATAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCTCCGCGCTGTAggg                                        <  1:2294658/100‑1 (MQ=255)

                                                     gAGTGGACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCTCCGCGCTGTAggg                                        <  2:1034966/100‑1 (MQ=255)

                                                       gTGGACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCTCCGCGCTGTAGGGTa                                      >  1:1799610/1‑100 (MQ=255)

                                                         ggACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCTCCGCGCTGTAGGGTAcc                                    <  1:266140/100‑1 (MQ=255)

                                                         ggACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCTCCGCGCTGTAGGGTAcc                                    <  1:2675540/100‑1 (MQ=255)

                                                         ggACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCTCCGCGCTGTAGGGTAcc                                    <  1:267518/100‑1 (MQ=255)

                                                         ggACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCTCCGCGCTGTAGGGTAcc                                    <  1:1860490/100‑1 (MQ=255)

                                                         ggACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCTCCGCGCTGTAGGGTAcc                                    <  1:1483534/100‑1 (MQ=255)

                                                         ggACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCTCCGCGCTGTAGGGTAcc                                    >  1:1259671/1‑100 (MQ=255)

                                                         cttccgatctaTCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCTCCGCGCTGTAGGGTAcc                                    <  1:1060203/90‑1 (MQ=255)

                                                          gACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCTCCGCGCTGTAGGGTACCa                                   >  1:1487469/1‑100 (MQ=255)

                                                            cTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCTCCGCGCTGTAGGGTACCAGt                                 >  1:1111382/1‑100 (MQ=255)

                                                            cTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCTCCGCGCTGTAGGGTACCAGt                                 >  2:1725668/1‑100 (MQ=255)

                                                              ccAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCTCCGCGCTGTAGGGTACCAGTGc                               <  2:1259661/100‑1 (MQ=255)

                                                              ccAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGAGAAGACTGGACGTCATTCACTAACTGATACATTTGCTCCGCGCTGTAGGGTACCAGTg                                >  2:793804/1‑99 (MQ=255)

                                                                aGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCTCCGCGCTGTAGGGTACCAGTGCgg                             <  1:1113653/100‑1 (MQ=255)

                                                                   aTCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCTCCGCGCTGTAGGGTACCAgatcggaag                          >  2:1060195/1‑92 (MQ=255)

                                                                   aTCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCTCCGCGCTGTAGGGTACCAGTGCGGTcc                          >  1:98815/1‑100 (MQ=255)

                                                                    tCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCTCCGCGCTGTAGGGTACCAGTGCGGTCCg                         >  2:2077758/1‑100 (MQ=255)

                                                                      caaCTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCTCCGCGCTGTAGGGTACCAGTGCGGTCCGGc                       >  1:1823397/3‑100 (MQ=255)

                                                                          taccGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCCCTAACTGATACATTTGCTCCGCGCTGTAGGGTACCAGTGCGGTCCGGTTAAt                   <  2:2527327/98‑1 (MQ=255)

                                                                           tCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCTCCGCGCTGTAGGGTACCAGTGCGGTCCGGCTAATc                  <  2:2168754/100‑1 (MQ=255)

                                                                           tCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCTCCGCGCTGTAGGGTACCAGTGCGGTCCGGCTAATc                  <  1:518936/100‑1 (MQ=255)

                                                                           tCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCTCCGCGCTGTAGGGTACCAGTGCGGTCCGGCTAATc                  <  2:1149404/100‑1 (MQ=255)

                                                                             cGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCTCCGCGCTGTAGGGTACCAGTGCGGTCCGGCTAATCTg                >  1:1380857/1‑100 (MQ=255)

                                                                              ggTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCTCCGCGCTGTAGGGTACCAGTGCGGTCCGGCTAATCTGa               >  1:2533861/1‑100 (MQ=255)

                                                                               gTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCTCCGCGCTGTAGGGTACCAGTGCGGTCCGGCTAATCTGAg              <  1:2159774/100‑1 (MQ=255)

                                                                               gTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCTCCGCGCTGTAGGGTACCAGTGCGGTCCGGCTAATCTGAg              <  2:702947/100‑1 (MQ=255)

                                                                                tACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCTCCGCGCTGTAGGGTACCAGTGCGGTCCGGCTAATCTGAgg             <  2:2554779/100‑1 (MQ=255)

                                                                                tACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCTCCGCGCTGTAGGGTACCAGTGCGGTCCGGCTAATCTGAgg             <  1:1405563/100‑1 (MQ=255)

                                                                                tACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCTCCGCGCTGTAGGGTACCAGTGCGGTCCGGCTAATCTGAgg             <  2:53354/100‑1 (MQ=255)

                                                                                tACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCTCCGCGCTGTAGGGTACCAGTGCGGTCCGGCTAATCTGAgg             <  1:2604139/100‑1 (MQ=255)

                                                                                 aCAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCTCCGCGCTGTAGGGTACCAGTGCGGTCCGGCTAATCTGAGGc            <  2:872261/100‑1 (MQ=255)

                                                                                 aCAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCTCCGCGCTGTAGGGTACCAGTGCGGTCCGGCTAATCTGAGGc            <  1:711486/100‑1 (MQ=255)

                                                                                 aCAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACATGTGCTCCGCGCTGTAGGGTACCAGTGCGGTCCGGCTAATCTGAGGc            <  2:339661/100‑1 (MQ=255)

                                                                                  cAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCTCCGCGCTGTAGGGTACCAGTGCGGTCCGGCTAATCTGAGGCa           >  2:560181/1‑100 (MQ=255)

                                                                                  cAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCTCCGCGCTGTAGGGTACCAGTGCGGTCCGGCTAATCTGAGGCa           >  2:955828/1‑100 (MQ=255)

                                                                                  cAACCAGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCTCCGCGCTGTAGGGTACCAGTGCGGTCCGGCTAATCTGAGGCa           >  2:1933468/1‑100 (MQ=255)

                                                                                    aCCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCTCCGCGCTGTAGGGTACCAGTGCGGTCCGGCTAATCTGaagatcg         >  1:975488/1‑94 (MQ=255)

                                                                                    aCCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCTCCGCGCTGTAGGGTACCAGTGCGGTCCGGCTAATCTGAGGCATa         >  2:1264433/1‑100 (MQ=255)

                                                                                    aCCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCTCCGCGCTGTAGGGTACCAGTGCGGTCCGGCTAATCTGAGGCATa         >  2:2554958/1‑100 (MQ=255)

                                                                                     cccGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCTCCGCGCTGTAGGGTACCAGTGCGGTCCGGCTAATCTGAGGCATaa        >  1:2385014/1‑100 (MQ=255)

                                                                                     cccGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCTCCGCGCTGTAGGGTACCAGTGCGGTCCGGCTAATCTGAGGCATaa        >  1:2386064/1‑100 (MQ=255)

                                                                                     cccGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCTCCGCGCTGTAGGGTACCAGTGCGGTCCGGCTAATCTGAGGCATaa        >  1:966176/1‑100 (MQ=255)

                                                                                      ccGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCTCCGCGCTGTAGGGTACCAGTGCGGTCCGGCTAATCTGAGGCATAAc       >  2:277987/1‑100 (MQ=255)

                                                                                          cAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCTCCGCGCTGTAGGGTACCAGTGCGGTCCGGCTAATCTGAGGCATAACAAtt   >  2:338598/1‑100 (MQ=255)

                                                                                          cAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCTCCGCGCTGTAGGGTACCAGTGCGGTCCGGCTAATCTGAGGCATAACAAtt   >  2:2396086/1‑100 (MQ=255)

                                                                                          cAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCTCCGCGCTGTAGGGTACCAGTGCGGTCCGGCTAATCTGAGGCATAACAAtt   >  2:1792384/1‑100 (MQ=255)

                                                                                           aaaaaaTGAGGATAAGACTGAACGTCATTTACTAACTGATACATTTGCTCCGCGCTGTAGGGTACCTGTGCGGTCCGGCTAATCTGAGGCATAACAAttt  <  2:1619401/100‑1 (MQ=255)

                                                                                           aaaaaaTGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCTCCGCGCTGTAGGGTACCAGTGCGGTCCGGCTAATCTGAGGCATAACAAttt  >  1:1906940/1‑100 (MQ=255)

CGTTTTGCTGATCCCAGCCTTAGAGACATCTACCGCCGCAGTCATCTGCCCAGGAGTGGACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAACTGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCTCCGCGCTGTAGGGTACCAGTGCGGTCCGGCTAATCTGAGGCATAACAATTT  >  NC_000913/2754543‑2754733

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`