Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,067,320 A→T 19.4% intergenic (‑147/+20) ygfI ← / ← yggE putative DNA‑binding transcriptional regulator/oxidative stress defense protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,067,3200AT19.4% 255.7 / 65.5 119intergenic (‑147/+20)ygfI/yggEputative DNA‑binding transcriptional regulator/oxidative stress defense protein
Reads supporting (aligned to +/- strand):  ref base A (49/47);  new base T (9/14);  total (58/61)
Fisher's exact test for biased strand distribution p-value = 3.58e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.05e-01

Only 100 of 127 total aligned reads displayed.

CCTTGATGAAACCGCGAGCAAAGATAGGTGATTACGTCATGGTTTTACAGAAAATTACAGAAAAAGGAGGCAATATCGGGTAAAGGCATTAGCCCGACGAATACGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCGGGTTGTTGATCCACAGGTTCTAACTGGAAGACCACATCGACCTGATCATCAA  >  NC_000913/3067221‑3067416
                                                                                                   |                                                                                                
ccTTGATGAAACCGCGAGCAAAGATAGGTGATTACGTCATGGTTTTACAGAAAATTACAGAAAAAGGAGGCAATATCGGGTAAAGGCATTAGCCcgacga                                                                                                  >  2:2930100/1‑100 (MQ=255)
ccTTGATGAAACCGCGAGCAAAGATAGGTGATTACGTCATGGTTTTACAGAAAATTACAGAAAAAGGAGGCAATATCGGGTAAAGGCATTAGCCcgacga                                                                                                  >  2:2450771/1‑100 (MQ=255)
ccTTGATGAAACCGCGAGCAAAGATAGGTGATTACGTCATGGTTTTACAGAAAATTACAGAAAAAGGAGGCAATATCGGGTAAAGGCATTAGCCcgacga                                                                                                  <  2:1914909/100‑1 (MQ=255)
 cTTGATGAAACCGCGAGCAAAGATAGGTGATTACGTCATGGTTTTACAGAAAATTACAGAAAAAGGAGGCAATATCGGGTAAAGGCATTAGCCCGACGaa                                                                                                 >  2:2165865/1‑100 (MQ=255)
 cTTGATGAAACCGCGAGCAAAGATAGGTGATTACGTCATGGTTTTACAGAAAATTACAGAAAAAGGAGGCAATATCGGGTAAAGGCATTAGCCCGACGaa                                                                                                 <  1:1970193/100‑1 (MQ=255)
  atcttgaAACCGCGAGCAAAGATAGGTGATTACGTCATGGTTTTACAGAAAATTACAGAAAAAGGAGGCAATATCGGGTAAAGGCATTAGCCCGACGAAt                                                                                                <  1:2794414/96‑1 (MQ=255)
   tgatgaAACCGCGAGCAAAGATAGGTGATTACGTCATGGTTTTACAGAAAATTACAGAAAAAGGAGGCAATATCGGGTAAAGGCATTAGCCCGACGTAtt                                                                                               <  1:2643968/100‑2 (MQ=255)
      tgaAACCGCGAGCAAAGATAGGTGATTACGTCATGGTTTTACAGAAAATTACAGAAAAAGGAGGCAATATCGGGTAAAGGCATTAGCCCGACGAATACGt                                                                                            >  1:2046093/1‑100 (MQ=255)
         aaCCGCGAGCAAAGATAGGTGATTACGTCATGGTTTTACAGAAAATTACAGAAAAAGGAGGCAATATCGGGTAAAGGCATTAGCCCGACGAATACGTc                                                                                           >  1:1976243/1‑98 (MQ=255)
         aaCCGCGAGCAAAGATAGGTGATTACGTCATGGTTTTACAGAAAATTACAGAAAAAGGAGGCAATATCGGGTAAAGGCATTAGCCCGACGAATACGTCg                                                                                          >  1:2739446/1‑99 (MQ=255)
          aCCGCGAGCAAAGATAGGTGATTACGTCATGGTTTTACAGAAAATTACAGAAAAAGGAGGCAGTATCGGGTAAAGGCATTAGCCCGACGTATTCGTCggg                                                                                        <  2:1963904/100‑1 (MQ=255)
          aCCGCGAGCAAAGATAGGTGATTACGTCATGGTTTTACAGAAAATTACAGAAAAAGGAGGCAATATCGGGTAAAGGCATTAGCCCGACGTATTCGTCggg                                                                                        <  2:948362/100‑1 (MQ=255)
          aCCGCGAGCAAAGATAGGTGATTACGTCATGGTTTTACAGAAAATTACAGAAAAAGGAGGCAATATCGGGTAAAGGCATTAGCCCGACGTATTCGTCggg                                                                                        <  2:1272602/100‑1 (MQ=255)
           ccGCGAGCAAAGATAGGTGATTACGTCATGGTTTTACAGAAAATTACAGAAAAAGGAGGCAATATCGGGTAAAGGCATTAGCCCGACGTATTCGTCggg                                                                                        <  1:2142172/99‑1 (MQ=255)
           ccGCGAGCAAAGATAGGTGATTACGTCATGGTTTTACAGAAAATTACAGAAAAAGGAGGCAATATCGGGTAAAGGCATTAGCCCGACGTATTCGTCGGGc                                                                                       <  2:1018267/100‑1 (MQ=255)
           ccGCGAGCAAAGATAGGTGATTACGTCATGGTTTTACAGAAAATTACAGAAAAAGGAGGCAATATCGGGTAAAGGCATTAGCCCGACGTATTCGTCGGGc                                                                                       <  2:3418507/100‑1 (MQ=255)
           ccGCGAGCAAAGATAGGTGATTACGTCATGGTTTTACAGAAAATTACAGAAAAAGGAGGCAATATCGGGTAAAGGCATTAGCCCGACGAATACGTCgg                                                                                         >  1:2719860/1‑98 (MQ=255)
           ccGCGAGCAAAGATAGGTGATTACGTCATGGTTTTACAGAAAATTACAGAAAAAGGAGGCAATATCGGGTAAAGGCATTAGCCCGACGAATACGTCGGGc                                                                                       <  2:135385/100‑1 (MQ=255)
           ccGCGAGCAAAGATAGGTGATTACGTCATGGTTTTACAGAAAATTACAGAAAAAGGAGGCAATATCGGGTAAAGGCATTAGCCCGACGAATACGTCGGGc                                                                                       <  1:118343/100‑1 (MQ=255)
           ccGCGAGCAAAGATAGGTGATTACGTCATGGTTTTACAGAAAATTACAGAAAAAGGAGGCAATATCGGGGAAAGGCATTAGCCCGACGTATTCGTCGGGc                                                                                       <  1:2293162/100‑1 (MQ=255)
            cgcgAGCAAAGATAGGTGATTACGTCATGGTTTTACAGAAAATTACAGAAAAAGGAGGCAATATCGGGTAAAGGCATTAGCCCGACGTATTCGTCGGGCt                                                                                      <  1:435964/100‑1 (MQ=255)
            cgcgAGCAAAGATAGGTGATTACGTCATGGTTTTACAGAAAATTACAGAAAAAGGAGGCAATATCGGGTAAAGGCATTAGCCCGACGTATTCGTCGGGCt                                                                                      <  1:2518354/100‑1 (MQ=255)
              cgAGCAAAGATAGGTGATTACGTCATGGTTTTACAGAAAATTACAGAAAAAGGAGGCAATATCGGGTAAAGGCATTAGCCCGACGTATTCGTCGGGCTaa                                                                                    <  2:3166647/100‑2 (MQ=255)
              cgAGCAAAGATAGGTGATTACGTCATGGTTTTACAGAAAATTACAGAAAAAGGAGGCAATATCGGGTAAAGGCATTAGCCCGACGAATACGTCGGGCTAc                                                                                    <  2:2387338/100‑1 (MQ=255)
                  cAAAGATAGGTGATTACGTCATGGTTTTACAGAAAATTACAGAAAAAGGAGGCAATATCGGGTAAAGGCATTAGCCCGACGAATACGTCGGGCTACAAat                                                                                >  1:3275052/1‑100 (MQ=255)
                  cAAAGATAGGTGATTACGTCATGGTTTTACAGAAAATTACAGAAAAAGGAGGCAATATCGGGTAAAGGCATTAGCCCGACGAATACGTCGGGCTACAAat                                                                                >  1:3194162/1‑100 (MQ=255)
                  cAAAGATAGGTGATTACGTCATGGTTTTACAGAAAATTACAGAAAAAGGAGGCAATATCGGGTAAAGGCATTAGCCCGACGAATACGTCGGGCTACAAat                                                                                >  2:1228683/1‑100 (MQ=255)
                  cAAAGATAGGTGATTACGTCATGGTTTTACAGAAAATTACAGAAAAAGGAGGCAATATCGGGTAAAGGCATTAGCCCGACGAATACGTCGGGCTACAAat                                                                                >  1:1439500/1‑100 (MQ=255)
                    aggATAGGTGGTTACGTCATGGTTTTAGAGATGATTAAAGAGAACGGAGGTGATATCGGGTATAGGCATTAGCGCGAAGAATAGGTCggg                                                                                        >  1:3243647/3‑90 (MQ=255)
                    aaGATAGGTGATTACGTCATGGTTTTACAGAAAATTACAGAAAAAGGAGGCAATATCGGGTAAAGGCATTAGCCCGACGAATACGTCGGGCTACAAatat                                                                              >  2:2831406/1‑100 (MQ=255)
                     aGATAGGTGATTACGTCATGGTTTTACAGAAAATTACAGAAAAAGGAGGCAATATCGGGTAAAGGCATTAGCCCGACGAATACGTCGGGCTACAAAtatt                                                                             <  2:2895375/100‑1 (MQ=255)
                         aGGTGATTACGTCATGGTTTTACAGAAAATTACAGAAAAAGGAGGCAATATCGGGTAAAGGCATTAGCCCGACGAATACGTCGGGCTACAAATATTATtg                                                                         >  1:1324103/1‑100 (MQ=255)
                          ggTGATTACGTCATGGTTTTACAGAAAATTACAGAAAAAGGAGGCAATATCGGGTAAAGGCATTAGCCCGACGAATACGTCGGGCTACAAATATTATtgt                                                                        >  1:1440680/1‑100 (MQ=255)
                           gTGATTGCGTCATGGTTTTACAGAAAATTACAGAAAAAGGAGGCAATAGCGGGTAAAGGCATTAGCCCGACGAATACGTCGGGCTACAAATATTATtgtg                                                                       <  2:1611967/100‑1 (MQ=255)
                           gTGATTACGTCATGGTTTTACAGAAAATTACAGAAAAAGGAGGCAATATCGGGTAAAGGCATTAGCCCGACGAATACGTCGGGCTACAAATATTATtgtg                                                                       >  2:3283475/1‑100 (MQ=255)
                            tGATTACGTCATGGTTTTACAGAAAATTACAGAAAAAGGAGGCAATATCGGGTAAAGGCATTAGCCCGACGAATACGTCGGGCTACAAATATTATTGtgc                                                                      <  2:2180253/100‑1 (MQ=255)
                            tGATTACGTCATGGTTTTACAGAAAATTACAGAAAAAGGAGGCAATATCGGGTAAAGGCATTAGCCCGACGAATACGTCGGGCTACAAATATTATTGtgc                                                                      <  1:1754026/100‑1 (MQ=255)
                            tGATTACGTCATGGTTTTACAGAAAATTACAGAAAAAGGAGGCAATATCGGGTAAAGGCATTAGCCCGACGAATACGTCGGGCTACAAATATTATTGtgc                                                                      <  1:1434386/100‑1 (MQ=255)
                             gATTACGTCATGGTTTTACAGAAAATTACAGAAAAAGGAGGCAATATCGGGTAAAGGCATTAGCCCGACGAATACGTCGGGCTACAAATATTATTGtgct                                                                     >  2:2752952/1‑100 (MQ=255)
                              aTTACGTCATGGTTTTACAGAAAATTACAGAAAAAGGAGGCAATATCGGGTAAAGGCATTAGCCCGACGAATACGTCGGGCTACAAATATTATTGtgctg                                                                    >  2:1894565/1‑100 (MQ=255)
                                   gTCATGGTTTTACAGAAAATTACAGAAAAAGGAGGCAATATCGGGTAAAGGCATTAGCCCGACGAATACGTCGGGCTACAAATATTATTGTGCTGCAGgt                                                               >  2:149016/1‑100 (MQ=255)
                                    tCATGGTTTTACAGAAAATTACAGAAAAAGGAGGCAATATCGGGTAAAGGCATTAGCCCGACGAATACGTCGGGCTACAAATATTATTGTGCTGCAGgtg                                                              >  2:2633480/1‑100 (MQ=255)
                                    tCATGGTTTTAAAGAAAATTACAGAAAAAGGAGGCAATATCGGGTAAAGGCATTAGCCCGACGAATACGTCGGGCTACAAATATTATTGTGCTGCAGgtg                                                              <  1:378905/100‑1 (MQ=255)
                                       tGGTTTTACAGAAAATTACAGAAAAAGGAGGCAATATCGGGTAAAGGCATTAGCCCGACGAATACGTCGGGCTACAAATATTATTGTGCTGCAGGTGttt                                                           >  2:1653773/1‑100 (MQ=255)
                                        ggTTTTACAGAAAATTACAGAAAAAGGAGGCAATATCGGGTAAAGGCATTAGCCCGACGAATACGTCGGGCTACAAATATTATTGTGCTGCAGGTGtttt                                                          <  2:2765999/100‑1 (MQ=255)
                                         gTTTTACAGAAAATTACAGAAAAAGGAGGCAATATCGGGTAAAGGCATTAGCCCGCCGAATACGTCGGGCTACAAATATTATTGTGCTGCCGGTGTTTTa                                                         >  2:2037422/1‑100 (MQ=255)
                                         gTTTTACAGAAAATTACAGAAAAAGGAGGCAATATCGGGTAAAGGCATTAGCCCGACGAATACGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTa                                                         >  1:2146682/1‑100 (MQ=255)
                                          ttttACAGAAAATTACAGAAAAAGGAGGCAATATCGGGTAAAGGCATTAGCCCGACGAATACGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAg                                                        <  2:1294484/100‑1 (MQ=255)
                                           tttACAGAAAATTACAGAAAAAGGAGGCAATATCGGGTAAAGGCATTAGCCCGACGAATACGTCGGGTTACAAATATTATTGTGCTGCAGGTGTTTTAGc                                                       >  1:1375687/1‑100 (MQ=255)
                                            ttacagaaaattacagaaaaAGGAGGCAATATCGGGTAAAGGCATTAGCCCGACGAATACGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCg                                                      <  1:43458/100‑1 (MQ=255)
                                             tacagaaaattacagaaaaAGGAGGCAATATCGGGTAAAGGCATTAGCCCGACGAATACGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCgg                                                     <  1:1631932/100‑1 (MQ=255)
                                                agaaaattacagaaaaAGGAGGCAATATCGGGTAAAGGCATTAGCCCGACGAATACGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCGGgtt                                                  >  1:2959530/1‑100 (MQ=255)
                                                  aaaattacagaaaaAGGAGGCAATATCGGGTAAAGGCATTAGCCCGACGAATACGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCGGgttgt                                                <  2:234192/100‑1 (MQ=255)
                                                      ttacagaaaaAGGAGGCAATATCGGGTAAAGGCATTAGCCCGACGAATACGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCGGGTTGTTGAt                                            <  1:1450641/100‑1 (MQ=255)
                                                       tacagaaaaAGGAGGCAATATCGGGTAAAGGCATTCGCCCGACGAATACGTCGGGCTACAAATATTATTGTGCTGCAGGTGTCTTCGCGGGTTGTTGATc                                           <  1:1536911/100‑1 (MQ=255)
                                                        acagaaaaAGGAGGCAATATCGGGTAAAGGCATTAGCCCGACGAATACGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCGGGTTGTTGATcc                                          <  2:2870111/100‑1 (MQ=255)
                                                         cagaaaaAGGAGGCAATATCGGGTAAAGGCATTAGCACGACGAATACGTCGGGCGACAAATATTATTGTGCTGCAGGTGTTTTAGCGGGTTGTTGATCca                                         >  1:3280073/1‑100 (MQ=255)
                                                          agaaaaAGGAGGCAATATCGGGTAAAGGCATTAGCCCGCCGAATACGTCGGGCTACAAGTATTATTGTGCTGCAGGTGTTTTAGCGGGTTGTTGACCcac                                        <  1:1864427/100‑1 (MQ=255)
                                                           gaaaaAGGAGGCAATATCGGGTAAAGGCATTAGCCCGACGAATACGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCGGGTTGTTGATCcaca                                       <  2:2764786/100‑1 (MQ=255)
                                                           gaaaaAGGAGGCAATATCGGGTAAAGGCATTAGCCCGACGAATACGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCGGGTTGTTGATCcaca                                       >  2:47656/1‑100 (MQ=255)
                                                             aaaaGGAGGCAATATCGGGTAAAGGCATTAGCCCGACGAATACGTCGGGCTACAAATATTATTGTGCTGCATGTGTTTTAGCGGGTTGTTGATCtac                                        <  2:3132868/97‑1 (MQ=255)
                                                                aggaggCAATATCGGGTAAAGGCATTAGCCCGACGAATACGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCGGGTTGTTGATCCACAGGTTc                                  <  1:1402385/100‑1 (MQ=255)
                                                                 ggagtCAATATCGTTCAAAGGCATTAGCCCGACGAATACGTCGGGCTACAAATAGTATTGTGCTGCAGGTGTTTTAGCGGGTTGTTGATCCACAGGTTc                                  <  1:1995409/99‑1 (MQ=255)
                                                                   aggCAATATCGGGTAAAGGCATTAGCCCGACGAATACGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCGGGTTGTTGATCCACAGGTTCTaa                               >  2:3390875/1‑100 (MQ=255)
                                                                    ggCAATATCGGGTAAAGGCATTAGCCCGACGAATACGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCGGGTTGTTGATCCACAGGTTCTAAc                              >  2:234041/1‑100 (MQ=255)
                                                                    ggCAATATAGGGTAACGGCATTAGCCCGACGAATAGGTCGGACTACAAATAATATTGGGCGGCAGGTGCTTGAGCGGGTTGTTGATCCGCTGGTCCTa                                >  1:3120852/1‑98 (MQ=255)
                                                                     gCAATATCGGGTAAAGGCATTAGCCCGACGAATACGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCGGGTTGTTGATCCACAGGTTCTAACt                             >  2:2780649/1‑100 (MQ=255)
                                                                     gCAATATCGGGTAAAGGCATTAGCCCGACGAATACGTCGGGCTACAAATATCATTGTGCTGCAGGTGTTTTAGCGGGTTGTTGATCCACAGGTTCTAAc                              <  1:2474462/99‑1 (MQ=255)
                                                                      cAATATCGGGTAAAGGCATTAGCCCGACGAATACGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCGGGTTGTTGATCCACAGGTTCTAACTg                            >  2:326179/1‑100 (MQ=255)
                                                                       aaTATCGGGTAAAGGCATTAGCCCGACGAATACGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCGGGTTGTTGATCCACAGGTTCTAACTgg                           >  2:2104518/1‑100 (MQ=255)
                                                                        atatCGGGTAAAGGCATTAGCCCGACGAATACGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCGGGTTGTTGATCCACAGGTTCTAACTGGa                          >  1:1769735/1‑100 (MQ=255)
                                                                        atatCGGGTAAAGGCATTAGCCCGACGAATACGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCGGGTTGTTGATCCACAGGTTCTAACTGGa                          <  2:743075/100‑1 (MQ=255)
                                                                         tatCGGGTAAAGGCATTAGCCCGACGAATACGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCGGGTTGTTGATCCACAGGTTCTAACTGGaa                         <  2:168404/100‑1 (MQ=255)
                                                                          atCGGGTAAAGGCATTAGCCCGACGAATACGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCGGGTTGTTGATCCACAGGTTCTAACTGGAAg                        <  1:406225/100‑1 (MQ=255)
                                                                          atCGGGTAAAGGCATTAGCCCGACGAATACGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCGGGTTGTTGATCCACAGGTTCTAACTGGAAg                        <  2:3011102/100‑1 (MQ=255)
                                                                           tCGGGTAAAGGCATTAGCCCGACGAATACGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCGGGTTGTTGATCCACAGGTTCTAACTGGAAGa                       <  1:1750890/100‑1 (MQ=255)
                                                                           tCGGGTAAAGGCATTAGCCCGACGAATACGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCGGGTTGTTGATCCACAGGTTCTAACTGGAAGa                       <  1:1365361/100‑1 (MQ=255)
                                                                            cGGGTAAAGGCATTAGCCCGACGAATACGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCGGGTTGTTGATCCACAGGTTCTAACTGGAAGAc                      <  2:1040048/100‑1 (MQ=255)
                                                                               gTAAAGGCATTAGCCCGACGAATACGTCGGGCTAAAAATATTATTGTGCTGCAGGTGTTTTAGCGGGTTGTTGATCCACAGGTTCTAACTGGAAGACcac                   >  1:1133690/1‑100 (MQ=255)
                                                                                 aaaGGCATTAGCCCGACGAATACGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCGGGTTGTTGATCCACAGGTTCTAACTGGAAGACCACAt                 <  2:1397880/100‑1 (MQ=255)
                                                                                 aaaGGCATTAGCCCGACGAATACGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCGGGTTGTTGATCCACAGGTTCTAACTGGAAGACCACAt                 <  2:1324109/100‑1 (MQ=255)
                                                                                  aaGGCATTAGCCCGACGAATACGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCGGGTTGTTGATCCACAGGTTCTAACTGGAAGACCACATc                >  1:2918364/1‑100 (MQ=255)
                                                                                   aGGCATTAGCCCGACGAATACGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCGGGTTGTTGATCCACAGGTTCTAACTGGAAGACCACATCg               >  1:1246573/1‑100 (MQ=255)
                                                                                      ttgtAGCCCGACGTATTCGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCGGGTTGTTGATCCACAGGTTCTAACTGGAAGACCACATCGAcc            >  2:1940158/4‑100 (MQ=39)
                                                                                        ttAGCCTGACGAATACGTCGGGCTACAAATATTATTTTGCTGCAGGTGTCTTAGCGGGTTGTTGATCCACAGGTTCTAACTGGAAGACCACATCGACCtt          <  1:1111459/100‑2 (MQ=255)
                                                                                        ttAGCCCGACGAATACGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCGGGTTGTTGATCCACAGGTTCTAACTGGAAGACCACATCGACCTg          >  1:1223219/1‑100 (MQ=255)
                                                                                        gtAGCCCGACGTATTCGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCGGGTTGTTGATCCACAGGTTCTAACTGGAAGACCACATCGACCTg          >  2:658547/2‑100 (MQ=255)
                                                                                         tAGCCCGACGTATTCGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCGGGTTGTTGATCCACAGGTTCTAACTGGAAGACCACATCGACCTGa         >  2:1536921/1‑100 (MQ=255)
                                                                                          aGCCCGACGAATACGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCGGGTTGTTGATCCACAGGTTCTAACTGGAAGACCACATCGaac            >  1:3077601/1‑94 (MQ=255)
                                                                                          aGCCCGACGAATACGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCGGGTTGTTGATCCACAGGTTCTAACTGGAAGACCACATCGACCTGat        >  2:57239/1‑100 (MQ=255)
                                                                                           gCCCGACGTATTCGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCGGGTTGTTGATCCACAGGTTCTAACTGGAAGACCACATCGACCTGatc       >  2:255338/1‑100 (MQ=255)
                                                                                           gCCCGACGTATTCGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCGGGTTGTTGATCCACAGGTTCTAACTGGAAGACCACATCGACCTGatc       >  2:1618197/1‑100 (MQ=255)
                                                                                           gCCCGACGTATTCGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCGGGTTGTTGATCCACAGGTTCTAACTGGAAGACCACATCGACCTGatc       >  2:466588/1‑100 (MQ=255)
                                                                                           gCCCGACGTATTCGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCGGGTTGTTGATCCACAGGTTCTAACTGGAAGACCACATCGACCTGatc       >  2:2792764/1‑100 (MQ=255)
                                                                                           gCCCGACGAATACGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCGGGTTGTTGATCCACAGGTTCTAACTGGAAGACCACATCGACCTGatc       >  2:1201382/1‑100 (MQ=255)
                                                                                           gCCCGACGAATACGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCGGGTTGTTGATCCACAGGTTCTAACTGGAAGACCACATCGACCTGatc       <  2:3292859/100‑1 (MQ=255)
                                                                                           gCCCGACGAATACGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCGGGTTGTTGATCCACAGGTTCTAACTGGAAGACCACATCGACCTGatc       >  2:1744587/1‑100 (MQ=255)
                                                                                              cgacgaATACGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCGGGTTGTTGATCCACAGGTTCTAACTGGAAGACCACATCGACCTGatcatc    >  1:1785644/1‑100 (MQ=255)
                                                                                               gacgaATACGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCGGGTTGTTGATCCACAGGTTCTAACTGGAAGACCACATCGACCTGAtcatca   <  1:632327/100‑1 (MQ=255)
                                                                                                acgaATACGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCGGGTTGTTGATCCACAGGTTCTAACTGGAAGACCACATCGACCTGATCATCaa  <  1:769340/100‑1 (MQ=255)
                                                                                                   |                                                                                                
CCTTGATGAAACCGCGAGCAAAGATAGGTGATTACGTCATGGTTTTACAGAAAATTACAGAAAAAGGAGGCAATATCGGGTAAAGGCATTAGCCCGACGAATACGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCGGGTTGTTGATCCACAGGTTCTAACTGGAAGACCACATCGACCTGATCATCAA  >  NC_000913/3067221‑3067416

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: