Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,338,928 C→A 100% L107F (TTG→TTT)  mlaE ← ABC transporter maintaining OM lipid asymmetry, inner membrane permease protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,338,9280CA100.0% 397.3 / ‑6.7 116L107F (TTG→TTT) mlaEABC transporter maintaining OM lipid asymmetry, inner membrane permease protein
Reads supporting (aligned to +/- strand):  ref base C (0/1);  major base A (49/65);  minor base G (1/0);  total (50/66)

Only 100 of 122 total aligned reads displayed.

CGGATCCACCGCCATCATCTCCATACTGGAGAGTTGCTCTGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAACAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATACCCAGACTGGTTTCCGCACTATAAGTGGTCAGAACCAGA  >  NC_000913/3338832‑3339024
                                                                                                |                                                                                                
cGGATCCACCGCCATCATCTCCATACTGGAGAGTTGCTCTGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAaaaaac                                                                                               <  2:2588611/100‑1 (MQ=255)
 ggATCCACCGCCATCATCTCCATACTGGAGAGTTGCTCTGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAaaaaaca                                                                                              >  1:3197934/1‑100 (MQ=255)
 ggATCCACCGCCATCATCTCCATACTGGAGAGTTGCTCTGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCAAGCAAAGCAaaaaaca                                                                                              <  1:2814689/100‑1 (MQ=255)
  gATCCACCGCCATCATCTCCATACTGGAGAGTTGCTCTGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAaaaaacaa                                                                                             >  1:1632100/1‑100 (MQ=255)
   aTCCACCGCCATCATCTCCATACTGGAGAGTTGCTCTGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAaaaaacaac                                                                                            <  2:1986939/100‑1 (MQ=255)
     ccACCGCCATCATCTCCNTACNGGAGAGTTGCTCTGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGNACCAGCNCGCCCGGCAAAAAACAAcgc                                                                                          <  1:2149164/100‑1 (MQ=255)
      cACCGCCATCATCTCCATACTGGAGAGTTGCTCTGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCACAAAACAAcgcg                                                                                         >  2:1440480/1‑100 (MQ=255)
      cACCGCCATCATCTCCATACTGGAGAGTTGCTCTGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAAcgcg                                                                                         >  2:2481850/1‑100 (MQ=255)
      cACCGCCATCATCTCCATACTGGAGAGTTGCTCTGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAAcgcg                                                                                         >  1:237452/1‑100 (MQ=255)
       aCCGCCATCATCTCCATACTGGAGAGTTGCTCTGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCgg                                                                                        <  2:1651615/100‑1 (MQ=255)
       aCCGCCATCATCTCCATACTGGAGAGTTGCTCTGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCgg                                                                                        <  2:2996856/100‑1 (MQ=255)
       aCCGCCATCATCTCCATACTGGAGAGTTGCTCTGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCgg                                                                                        >  2:463549/1‑100 (MQ=255)
        ccGCCATCATCTCCATACTGGAGAGTTGCTCTGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGc                                                                                       >  1:2351371/1‑100 (MQ=255)
           ccATCATCTCCATACTGGAGAGTTGCTCTGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAac                                                                                    <  2:1581197/100‑1 (MQ=255)
           ccATCATCTCCATACTGGAGAGTTGCTCTGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAac                                                                                    <  1:1149556/100‑1 (MQ=255)
            catcatCTCCATACTGGAGAGTTGCTCTGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAacc                                                                                   <  2:3096775/100‑1 (MQ=255)
            catcatCTCCATACTGGAGAGTTGCTCTGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAacc                                                                                   >  2:1058058/1‑100 (MQ=255)
             atcatcTCCATACTGGAGAGTTGCTCTGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAacca                                                                                  >  2:1866166/1‑100 (MQ=255)
               catcTCCATACTGGAGAGTTGCTCTGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAaccacc                                                                                >  1:2958758/1‑100 (MQ=255)
                 tctcCATACTGGAGAGTTGCTCTGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCgg                                                                              >  1:12422/1‑100 (MQ=255)
                 tctcCATACTGGAGAGTTGCTCTGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCgg                                                                              >  1:12223/1‑100 (MQ=255)
                  ctcCATACTGGAGAGTTGCTCTGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGc                                                                             <  1:1840675/100‑1 (MQ=255)
                   tcCATACTGGAGAGTTGCTCTGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGcc                                                                            <  1:2861147/100‑1 (MQ=255)
                   tcCATACTGGAGAGTTGCGCTGTAGCGCGCATCAGGCCGATTTCGGCGGTTAGCGCCGAACCAGCAAGAACGGCAAAAAACAACGCGGCAACCACCGGcc                                                                            <  2:1236338/100‑1 (MQ=255)
                    ccATACTGGAGAGTTGCTCTGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGccc                                                                           >  2:1615773/1‑100 (MQ=255)
                     cATACTGGAGAGTTGCTCTGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGcccc                                                                          <  1:1975775/100‑1 (MQ=255)
                     cATACTGGAGAGTTGCTCTGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGcccc                                                                          <  2:2493206/100‑1 (MQ=255)
                     cATACTGGAGAGTTGCTCTGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGcccc                                                                          >  2:2645087/1‑100 (MQ=255)
                      aTACTGGAGAGTTGCTCTGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCa                                                                         >  1:2639347/1‑100 (MQ=255)
                       tACTGGTGAGTTGCGCAGTAGCGCGCCTCAGGCTGATCTCTGCGGGTAGCGGCGAACCAGCCCGCCCGGCAAACAACAACGCGGCAACCACCGGccc                                                                           <  1:1338723/97‑1 (MQ=255)
                       tACTGGAGAGTTGCTCTGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAg                                                                        <  1:1374977/100‑1 (MQ=255)
                       tACTGGAGAGTTGCTCTGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAg                                                                        >  1:2999449/1‑100 (MQ=255)
                         cttgtgtgTTTCTCTGGAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGcc                                                                            <  2:3194838/87‑1 (MQ=255)
                         cTGGAGAGTTGCTCTGTAGCGCGCATCAGGCCGAGTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACCACGCGGCAACCACCGCcccccg                                                                        >  1:371406/1‑96 (MQ=255)
                           ggAGAGTTGCTCTGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCa                                                                    <  2:1317325/100‑1 (MQ=255)
                             agagTTGCTCTGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACg                                                                  >  2:1557240/1‑100 (MQ=255)
                             agagTTGCTCTGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACg                                                                  <  2:2510345/100‑1 (MQ=255)
                             agagTTGCTCTGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACg                                                                  <  2:3276747/100‑1 (MQ=255)
                             agagTTGCTCTGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACg                                                                  >  1:1177334/1‑100 (MQ=255)
                             agagTTGCTCTGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACg                                                                  <  1:2297650/100‑1 (MQ=255)
                               agTTGCTCTGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCa                                                                <  1:3264316/100‑1 (MQ=255)
                               agTTGCTCTGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCa                                                                <  2:1632569/100‑1 (MQ=255)
                               aattGCTCTGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCa                                                                >  2:2318097/3‑100 (MQ=255)
                                  tGCTCTGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTa                                                             <  2:3262160/100‑1 (MQ=255)
                                   gCTCTGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAg                                                            <  2:2805696/100‑1 (MQ=255)
                                    ctctGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGc                                                           <  1:1333183/100‑1 (MQ=255)
                                    ctctGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGc                                                           >  2:1766887/1‑100 (MQ=255)
                                     tctGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGTAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCg                                                          <  2:3345680/100‑1 (MQ=255)
                                       tGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGAt                                                        >  2:2163940/1‑100 (MQ=255)
                                          aGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCCATAAc                                                     >  1:2736729/1‑100 (MQ=255)
                                            cgcgcATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGc                                                   >  1:1609432/1‑100 (MQ=255)
                                              cgcATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAACACACCCGGCAAAAAATAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGcca                                                 >  2:194248/1‑100 (MQ=255)
                                               gcATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGccac                                                <  2:1969550/100‑1 (MQ=255)
                                               gcATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGccac                                                <  2:188329/100‑1 (MQ=255)
                                               gcATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGccac                                                <  2:695962/100‑1 (MQ=255)
                                                  tCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAg                                             <  2:451726/100‑1 (MQ=255)
                                                   cAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGc                                            <  2:2722774/100‑1 (MQ=255)
                                                    aGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCa                                           <  2:2736746/100‑1 (MQ=255)
                                                     ggCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCAt                                          >  2:2593857/1‑100 (MQ=255)
                                                       ccGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCGGTTCACGCAGTAGCGATAACGCCACCAGCATAc                                        >  2:1290888/1‑100 (MQ=255)
                                                         gATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCCAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATAccc                                      >  2:2409259/1‑100 (MQ=255)
                                                         gATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATAccc                                      >  2:2955093/1‑100 (MQ=255)
                                                         gATTTCTGCGGGTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCACCCACGGGCCCCAGTGCACGCAGTAGCGCTAACGCCACCAGccg                                          >  1:882113/1‑94 (MQ=255)
                                                            ttCTGCGGTTAGCGCCGAACCAGCCCGCACGGCAAAACACAACGCGGCAACCACCGGCCCCAGTTAACGCAGTAGCGATAACGCCACCAGCATACCCAg                                    <  1:743654/99‑1 (MQ=255)
                                                            ttCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATACCCAGa                                   >  1:1636880/1‑100 (MQ=255)
                                                             tCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATACCCAGAc                                  <  2:1663166/100‑1 (MQ=255)
                                                             tCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATACCCAGAc                                  <  1:3254060/100‑1 (MQ=255)
                                                              cTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATACCCAGAc                                  <  1:1679773/99‑1 (MQ=255)
                                                              cTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATACCCAGACt                                 >  1:741312/1‑100 (MQ=255)
                                                                gCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATACCCAGACTgg                               >  1:1519534/1‑100 (MQ=255)
                                                                 cGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATACCCAGACTGGt                              <  2:3123681/100‑1 (MQ=255)
                                                                 cGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATACCCAGACTGGt                              <  1:3337231/100‑1 (MQ=255)
                                                                   gTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATACCCAGACTGGttt                            >  2:829285/1‑100 (MQ=255)
                                                                    ttAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATACCCAGACTGGTTTc                           <  1:2293420/100‑1 (MQ=255)
                                                                    ttAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATACCCAGACTGGTTTc                           <  2:1456711/100‑1 (MQ=255)
                                                                     tAGCGCCGAACCAGCACGCCCGGCAAAAAACAGCGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATACCCAGACTGGTTTcc                          >  2:3245358/1‑100 (MQ=255)
                                                                     tAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCCCCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATACCCAGACTGGTTTcc                          <  1:2518905/100‑1 (MQ=255)
                                                                     tAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATACCCAGACTGGTTTcc                          <  2:1016222/100‑1 (MQ=255)
                                                                       gcgcCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATACCCAGACTGGTTTCCGc                        <  2:2233523/100‑1 (MQ=255)
                                                                       gcgcCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATACCCAGACTGGTTTCCGc                        >  2:1289781/1‑100 (MQ=255)
                                                                         gcCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATACCCAGACTGGTTTCCGCAc                      >  1:1722712/1‑100 (MQ=255)
                                                                         gcCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATACCCAGACTGGTTTCCGCAc                      >  2:9072/1‑100 (MQ=255)
                                                                         gcCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATACCCAGACTGGTTTCCGCAc                      <  2:1773306/100‑1 (MQ=255)
                                                                          ccGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATACCCAGACTGGTTTCCGCACt                     <  1:2506139/100‑1 (MQ=255)
                                                                            gAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATACCCAGACTGGTTTCCGCACtat                   >  2:2089367/1‑100 (MQ=255)
                                                                            gAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATACCCAGACTGGTTTCCGCACtat                   <  1:3210402/100‑1 (MQ=255)
                                                                            gAACCAGCACGCCCGGCAAAAAACAAAGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATACCCAGACTGGTTTCCGCACtat                   >  2:2088348/1‑100 (MQ=255)
                                                                               ccAGCACGCCCGGCAAAGAACAACGCGGCAACCACCGGCTCCAGGTCACGCCGTAGCGATAACGCCACACGCATACCCAGACTGGCTTCCCCAcacta                  >  1:1904092/1‑94 (MQ=255)
                                                                               ccAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATACCCAGACTGGTTTCCGCACTATAAg                <  2:2639363/100‑1 (MQ=255)
                                                                                cAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATACCCAGACTGGTTTCCGCACTATAAGt               <  1:3072340/100‑1 (MQ=255)
                                                                                 aGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATACCCAGACTGGTTTCCGCACTATAAGTg              >  1:1294511/1‑100 (MQ=255)
                                                                                    aCGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATACCCAGACTGGTTTCCGCACTATAAGTGGTc           <  2:1519541/100‑1 (MQ=255)
                                                                                     cGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATACCCAGACTGGTTTCCGCACTATAAGTGGTCa          >  2:1413692/1‑100 (MQ=255)
                                                                                     cGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATACCCAGACTGGTTTCCGCACTATAAGTGGTCa          <  1:1900327/100‑1 (MQ=255)
                                                                                      gCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATACCCAGACTGGTTTCCGCACTATAAGTGGTCAg         <  2:3414103/100‑1 (MQ=255)
                                                                                       cccGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCTACAAGCATAACCAGACTGGTTTCCGCACTATAAGTGGTCAGa        >  1:2397750/1‑100 (MQ=255)
                                                                                          gtcAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATACCCAGACTGGTTTCCCCACTATAAGTGGTCAGAAcc     <  1:2080594/98‑1 (MQ=255)
                                                                                          ggCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATACCCAGACTGGTTTCCGCACTATAAGTGGTCAGAAcc     <  1:1439405/100‑1 (MQ=255)
                                                                                           gCAAAAAACAACGCGGCAACCCCCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATTCCCAGACTGGTTTCCGCACTATAAGTGGTCAGAACCa    <  2:966831/100‑1 (MQ=255)
                                                                                           gCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATACCCAGACTGGTTTCCGCACTATAAGTGGTCAGAACCa    <  2:3342176/100‑1 (MQ=255)
                                                                                                |                                                                                                
CGGATCCACCGCCATCATCTCCATACTGGAGAGTTGCTCTGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAACAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATACCCAGACTGGTTTCCGCACTATAAGTGGTCAGAACCAGA  >  NC_000913/3338832‑3339024

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑