Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,706,017 C→T 23.4% intergenic (‑227/+81) dppB ← / ← dppA dipeptide/heme ABC transporter permease/dipeptide/heme ABC transporter periplasmic binding protein; dipeptide chemotaxis receptor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,706,0170CT23.4% 369.2 / 96.7 185intergenic (‑227/+81)dppB/dppAdipeptide/heme ABC transporter permease/dipeptide/heme ABC transporter periplasmic binding protein; dipeptide chemotaxis receptor
Reads supporting (aligned to +/- strand):  ref base C (61/68);  new base T (38/2);  total (115/70)
Fisher's exact test for biased strand distribution p-value = 9.99e-09
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.49e-01

Only 100 of 188 total aligned reads displayed.

GCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATT‑‑‑TTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCTTTGCCATCAGTCTTGTATGGCTTTTAATTATTCGATAGAGACGTTT  >  NC_000913/3705918‑3706116
                                                                                                    |                                                                                                            
   ccACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACAtt                                                                                                           >  2:1342879/1‑100 (MQ=255)
     aCCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTgn                                                                                                         <  2:2788830/100‑2 (MQ=255)
      ccGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCa                                                                                                        >  2:1827001/1‑100 (MQ=255)
      ccGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCa                                                                                                        >  2:175748/1‑100 (MQ=255)
       cGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAg                                                                                                      >  2:1575285/1‑100 (MQ=255)
         ggTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGaa                                                                                                   >  2:1524677/1‑100 (MQ=255)
         ggTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGaa                                                                                                   >  2:1101737/1‑100 (MQ=255)
            ataCAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAAt                                                                                                  <  1:3301264/98‑1 (MQ=255)
             taCAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAAtt‑‑‑tt                                                                                          >  2:771530/1‑100 (MQ=255)
               cAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATT‑‑‑TTC‑‑g                                                                                      <  2:2497041/100‑1 (MQ=255)
               cAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATT‑‑‑TTC‑‑g                                                                                      <  1:2202870/100‑1 (MQ=255)
               cAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATT‑‑‑TTC‑‑g                                                                                      <  1:1891880/100‑1 (MQ=255)
               cAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATT‑‑‑TTC‑‑g                                                                                      <  1:771528/100‑1 (MQ=255)
               cAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATT‑‑‑TTC‑‑g                                                                                      <  1:1736690/100‑1 (MQ=255)
               cAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATT‑‑‑TTC‑‑g                                                                                      <  1:155391/100‑1 (MQ=255)
                 gATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATT‑‑‑TTC‑‑GTa                                                                                    >  2:438803/1‑100 (MQ=255)
                 gATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATT‑‑‑TTC‑‑GTa                                                                                    >  1:1176200/1‑100 (MQ=255)
                     gttgACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATT‑‑‑TTC‑‑GTAGGcc                                                                                >  1:2951213/1‑100 (MQ=255)
                     gttgACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATT‑‑‑TTC‑‑GTAGGcc                                                                                >  2:2848465/1‑100 (MQ=255)
                     gttgACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATT‑‑‑TTC‑‑GTAGGcc                                                                                >  2:3092866/1‑100 (MQ=255)
                       tgACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATT‑‑‑TTC‑‑GTAGGCCTg                                                                              >  2:1685756/1‑100 (MQ=255)
                               tGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATT‑‑‑TTC‑‑GTAGGCCTGATAAGCgg                                                                      <  1:3365549/100‑1 (MQ=255)
                                gcgcTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATT‑‑‑TTC‑‑GTAGGCCTGATAAGCGGa                                                                     >  2:1429063/1‑100 (MQ=255)
                                   cTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATT‑‑‑TTC‑‑GTAGGCCTGATAAGCGGAgcg                                                                  <  1:1406199/100‑1 (MQ=255)
                                   cTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATT‑‑‑TTC‑‑GTAGGCCTGATAAGCGGAgcg                                                                  >  1:2806088/1‑100 (MQ=255)
                                    attttgtaggCCGGATAAGGCGTTTACGCCGCATCCGGCCTACAAAATCGTGCTAATTCAATATATTGCAGAAAC‑‑‑CTT‑‑GTAGGCCTGATACGCGTAgcgc                                                                 >  1:3193611/10‑100 (MQ=17)
                                     ttGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATT‑‑‑TTC‑‑GTAGGCCTGATAAGCGGAGCGCa                                                                <  1:751435/100‑1 (MQ=255)
                                     ttGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATT‑‑‑TTC‑‑GTAGGCCTGATAAGCGGAGCGCa                                                                <  2:2872182/100‑1 (MQ=255)
                                      tttgtaggCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAATCATGCAAATCCAAAATATTGCAGGAGT‑‑‑TAT‑‑GTAGGCCTGATAAGCGTAGCGCATc                                                              >  2:3131942/8‑100 (MQ=21)
                                      tGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATT‑‑‑TTC‑‑GTAGGCCTGATAAGCGGAgcg                                                                  <  2:2036993/97‑1 (MQ=255)
                                      tGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATT‑‑‑TTC‑‑GTAGGCCTGATAAGCGGAGCGCAt                                                               >  2:1724467/1‑100 (MQ=255)
                                       ttgtaggCCGGATAAGGCGTTTACGCCGCATCTGGCCTACAAATTCTTCCAAATTCAATATATTGCAGAAAT‑‑‑CAT‑‑GTAGGCCTGATAAGCGTAGCGCATc                                                              >  2:2922567/7‑100 (MQ=18)
                                       gTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATT‑‑‑TTC‑‑GTAGGCCTGATAAGCGGAGCGCATc                                                              >  2:2016511/1‑100 (MQ=255)
                                        tgtaggCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAAACCTTGCTAATTCAATATATTGCAGGGACT‑‑‑AT‑‑GTAGGCCTGATAAGCATAGCGCATCa                                                             >  2:2954177/6‑100 (MQ=18)
                                          taggCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAAAATCTTGCCAATTCAATATATTGCAGGACC‑‑‑AT‑‑GTAGGCCTGATAAGCGTAGCGCATCAgg                                                           >  2:910925/4‑100 (MQ=18)
                                           aggCCGGATAAGGCGTTTACGCCGCATCCGGCCTACAAATTCTGCAAATTCAACAAATTGCGAATC‑‑‑CCTT‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCa                                                         >  2:223702/3‑100 (MQ=18)
                                           aggCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAAACCTTGCTAATTCAATATATTGCAGGGAC‑‑‑TAT‑‑GTAGGCCTGATAAGCATAGCGCATCAGGc                                                          >  2:831/3‑100 (MQ=18)
                                           aTGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATT‑‑‑TTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGc                                                          <  1:1201060/100‑1 (MQ=255)
                                            ngCCGGATAAGGCGTTCGCGCCGCATCCGGCCTACAAAAGTTTGCAAATTCAATAAATTGCAGAATT‑‑‑CAT‑‑GTAGGCCTGATAAGCGAAGCGCATCAGGCa                                                         >  1:437202/2‑100 (MQ=17)
                                            ggCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAAGTCGTATAAATTCAATGTATTGCAGTTTA‑‑‑TTG‑‑GTAGGCCTGATAAGCGCAGCGCATCAGGCa                                                         >  2:2904754/2‑100 (MQ=21)
                                            ggCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAAGTCGTATAAATTCAATGTATTGCAGTTTA‑‑‑TTG‑‑GTAGGCCTGATAAGCGCAGCGCATCAGGCa                                                         >  1:2454328/2‑100 (MQ=21)
                                             gtcggataaggcgTTCACGCCGCATCCGACCCACAAAGTCTTGCAAAATCAATAAATTGCAGGAAC‑‑‑GAT‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCaa                                                        >  1:236624/10‑99 (MQ=21)
                                             gCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATT‑‑‑TTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCAt                                                        >  2:60731/1‑100 (MQ=255)
                                             gCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATT‑‑‑TTC‑‑GTAGGCCTGATAAGCGGAGAGAATCAGGCAt                                                        >  2:280631/1‑100 (MQ=255)
                                             gCCGGATAAGGCGTTTACGCCGCATCCGGCCTACAAATTCTGCAAATTCAACAAATTGCGAATCC‑‑‑CTT‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCAtt                                                       >  2:721850/1‑100 (MQ=18)
                                             gCCGGATAAGGCGTTTACGCCGCATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTT‑‑GTAGGCCTGATAAGCGCAGCGCATCAGGCa                                                         >  1:1845889/1‑100 (MQ=21)
                                             gCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAAACTTTGCAAATTCAATATATTGCAATC‑‑‑TCCGTGTAGGCCTGATAAGCGTAGCGCATCAGGCaa                                                        >  2:58121/1‑99 (MQ=18)
                                              ccggataaggcgTTTACGCCGCATCCGGCCTACAAATTCTGCAAATTCAACAAATTGCGAATCC‑‑‑CTT‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCAttt                                                      >  1:2737739/9‑100 (MQ=18)
                                               cggataaggcgTTTACGCCGCATCCGGCCTACAAAAGCATGCAAATTCAATATATTGCAGAGTT‑‑‑CAC‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCagtt                                                      >  2:3009341/8‑97 (MQ=17)
                                               cGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATT‑‑‑TTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCAttt                                                      >  2:3400471/1‑100 (MQ=255)
                                                ggataaggcgTTTACGCCGCATCTGGCCTACAAATTCTTCCAAATTCAATATATTGCAGAAAT‑‑‑CAT‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCAattt                                                     >  1:115540/7‑100 (MQ=18)
                                                ggataaggcgTTTACGCCGCATCCGGCCTACAAATTCTGCAAATTCAACAAATTGCGAATCC‑‑‑CTT‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCAtttgc                                                    >  1:1966237/7‑98 (MQ=18)
                                                ggataaggcgTTTACGCCGCATCCGGCCTACAAAAGCGTGTAAATTCAATACATTGCATGGGC‑‑‑CAT‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCAattt                                                     >  1:41950/7‑100 (MQ=17)
                                                ggataaggcgTTTACGCCGCATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTT‑‑GTAGGCCTGATAAGCGCAGCGCATCAGGCaatt                                                      >  1:2025235/7‑97 (MQ=21)
                                                ggataaggcgTTCACGCCGCATCCGGCCTACAAAAATCTTGCCAATTCAATATATTGCAGGACC‑‑‑AT‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCAgttt                                                     >  1:235105/7‑100 (MQ=18)
                                                ggataaggcgTTCACGCCGCATCCGGCCTACAAAGTCGTATAAATTCAATGTATTGCAGTTTA‑‑‑TTG‑‑GTAGGCCTGATAAGCGCAGCGCATCAGGCaatct                                                     >  2:1084949/7‑96 (MQ=25)
                                                ggataaggcgTTCACGCCGCATCCGGCCTACAAAACCTTGCTAATTCAATATATTGCAGGGACT‑‑‑AT‑‑GTAGGCCTGATAAGCATAGCGCATCAGGCagctt                                                     >  2:2506337/7‑96 (MQ=21)
                                                 gataaggcgTTTACGCCGCATCCGGCCTACAAAACAGTGCAAATTCAATATATTGCAATCTT‑‑‑CTG‑‑GTAGGCCTGATAAGCATCGCGCATCAGGCaagctt                                                    >  2:982579/6‑95 (MQ=21)
                                                 gataaggcgTTCACGCCGCATCCGGCCTACAAAAACATGCAAATTCAATAGATTGCAGAGATT‑‑‑AT‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCAatttt                                                    >  2:147905/6‑100 (MQ=17)
                                                  aTGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATT‑‑‑TTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTtttg                                                   <  2:2055126/100‑1 (MQ=255)
                                                   taaggcgTTCACGCCGCATCCGGCCTACAAAAGCACGCAAACTCAATATATTGCAGAGATC‑‑‑AT‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCAatttagc                                                  >  2:3190606/4‑97 (MQ=17)
                                                   tGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATT‑‑‑TTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTtttgc                                                  >  2:2198868/1‑100 (MQ=255)
                                                    aaggcgTTTACGCCGCATCCGGCCTACAAATTCTGCAAATTCAACAAATTGCGAATCC‑‑‑CTT‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCAtttgcgccc                                                >  1:2443099/3‑94 (MQ=21)
                                                     cggcgTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATT‑‑‑TTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTtttgcct                                                >  1:532750/1‑100 (MQ=255)
                                                      ggcgTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATT‑‑‑TTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTtttgcctt                                               >  2:636271/1‑100 (MQ=255)
                                                         gTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATT‑‑‑TTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTtttgcctttgc                                            <  1:2766626/100‑1 (MQ=255)
                                                          ttAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATT‑‑‑TTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTtttgcctttgcc                                           <  2:798264/100‑1 (MQ=255)
                                                                ccTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATT‑‑‑TTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCTTTGCCATCAGt                                     <  1:1766074/100‑1 (MQ=255)
                                                                ccTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATT‑‑‑TTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCTTTGCCATCAGt                                     >  1:2741244/1‑100 (MQ=255)
                                                                ccTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATT‑‑‑TTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCTTTGCCATCAGt                                     >  2:2131044/1‑100 (MQ=255)
                                                                 cTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATT‑‑‑TTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCTTTGCCATCAGTc                                    >  2:2753732/1‑100 (MQ=255)
                                                                  ttATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATT‑‑‑TTT‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCTTTGCCATCAGTCt                                   >  2:3385853/1‑100 (MQ=255)
                                                                  ttATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATT‑‑‑TTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCTTTGCCATCAGTCt                                   <  2:1032330/100‑1 (MQ=255)
                                                                    aTCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATT‑‑‑TTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCTTTGCCATCAGTCTTg                                 <  2:809253/100‑1 (MQ=255)
                                                                      ccGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATT‑‑‑TTC‑‑GTAGGCCTGATAAGCGGATCGCATCAGGCATTTTTGCCTTTGCCATCAGTCTTGTa                               <  1:1826993/100‑1 (MQ=255)
                                                                       cGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATT‑‑‑TTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCTTTGCCATCAGTCTTGTAt                              <  1:615911/100‑1 (MQ=255)
                                                                       cGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATT‑‑‑TTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCTTTGCCATCAGTCTTGTAt                              <  2:2653136/100‑1 (MQ=255)
                                                                           cTACAAAATCGTGCAAATTCAATACATTGCAGAATT‑‑‑TTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCTTTGCCATCAGTCTTGTATGGCt                          <  2:2203091/100‑1 (MQ=255)
                                                                            tACAAAATCGTGCAAATTCAATACATTGCTGAATT‑‑‑TTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGTATTTTTGCCTTTGCCATCAGTCTTGTATGGCtt                         <  1:2061347/100‑1 (MQ=255)
                                                                            tACAAAATCGTGCAAATTCAATACATTGCAGAATT‑‑‑TTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCTTTGCCATCAGTCTTGTATGGCtt                         <  1:1332952/100‑1 (MQ=255)
                                                                             aCAAAATCGTGCAAATTCAATACATTGCAGAATT‑‑‑TTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTTCTTTTGTTATCAGTCTTGTATGGCttt                        >  1:1666071/1‑100 (MQ=25)
                                                                              cAAAATCGTGCAAATTCAATACATTGCAGAATT‑‑‑TTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCTTTGCCATCAGTCTTGTATGGCtttt                       >  1:1070762/1‑100 (MQ=255)
                                                                                 aaaTCGTTCAAATTCAATACATTGCAGAATT‑‑‑TTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCTTTGCCATCAGTCTTGTATGGCTTTTaa                     <  1:2152895/100‑1 (MQ=255)
                                                                                  aaTCGTGCAAATTCAATACATTGCAGAATT‑‑‑TTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCTTTGCCATCAGTCTTGTATGGCTTTTAat                    <  2:2365425/100‑1 (MQ=255)
                                                                                    tCGTGCAAATTCAATACATTGCAGAATT‑‑‑TTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCTTTGCCATCAGTCTTGTATGGCTTTTAatta                  <  1:355783/100‑1 (MQ=255)
                                                                                     cGTGCAAATTCAATACATTGCAGAATT‑‑‑TTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCTTTGCCATCAGTCTTGTATGGCTTTTAattat                 >  1:39255/1‑100 (MQ=255)
                                                                                      gTGCAAATTCAATACATTGCAGAATT‑‑‑TTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCTTTGCCATCAGTCTTGTATGGCTTTTAattatt                >  1:251217/1‑100 (MQ=255)
                                                                                       tGCAAATTCAATACATTGCAGAATT‑‑‑TTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCTTTGCCATCAGTCTTGTATGGCTTTTAATTATTc               <  1:1129711/100‑1 (MQ=255)
                                                                                       tGCAAATTCAATACATTGCAGAATT‑‑‑TTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCTTTGCCATCAGTCTTGTATGGCTTTTAATTATTc               <  1:2250687/100‑1 (MQ=255)
                                                                                       tGCAAATTCAATACATTGCAGAATT‑‑‑TTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCTTTGCCATCAGTCTTGTATGGCTTTTAATTATTc               >  1:1823842/1‑100 (MQ=255)
                                                                                        gCAAATTCAATACATTGCAGAATT‑‑‑TTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCTTTGCCATCAGTCTTGTATGGCTTTTAATTATTCg              <  1:1069124/100‑1 (MQ=255)
                                                                                        gCAAATTCAATACATTGCAGAATT‑‑‑TTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCTTTGCCATCAGTCTTGTATGGCTTTTAATTATTCg              <  1:2040945/100‑1 (MQ=255)
                                                                                        gCAAATTCAATACATTGCAGAATT‑‑‑TTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCTTTGCCATCAGTCTTGTATGGCTTTTAATTATTCg              <  2:1150607/100‑1 (MQ=255)
                                                                                         cAAATTCAATACATTGCAGAATT‑‑‑TTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCTTTGCCATCAGTCTTGTATGGCTTTTAATTATTCGa             <  2:3322459/100‑1 (MQ=255)
                                                                                         cAAATTCAATACATTGCAGAATT‑‑‑TTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCTTTGCCATCAGTCTTGTATGGCTTTTAATTATTCGa             >  2:2472563/1‑100 (MQ=255)
                                                                                          aaaTTCAATACATTGCAGAATT‑‑‑TTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCTTTGCCATCAGTCTTGTATGGCTTTTAATTATTCGAt            <  1:3178742/100‑1 (MQ=255)
                                                                                           aaTTCAATACATTGCAGAATT‑‑‑TTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCTTTGCCATCAGTCTTGTATGGCTTTTAATTATTCGATa           <  1:1602566/100‑1 (MQ=255)
                                                                                              tCAATACATTGCAGAATT‑‑‑TTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCTTTGCCATCAGTCTTGTATGGCTTTTAATTATTCGATagag        <  1:287265/100‑1 (MQ=255)
                                                                                               cAATACATTGCAGAATT‑‑‑TTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCTTTGCCATCAGTCTTGTATGGCTTTTAATTATTCGATAgaga       <  1:522501/100‑1 (MQ=255)
                                                                                                    cATTGCAGAATT‑‑‑TTC‑‑GTAGGCCTGATAAGCTGAGCGCATCAGGCATTTTTGCCTTTGCCATCAGTCTTGGATGGCTTTTAATTATTCGATAGAGACGtt   <  1:696498/99‑1 (MQ=255)
                                                                                                    |                                                                                                            
GCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATT‑‑‑TTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCTTTGCCATCAGTCTTGTATGGCTTTTAATTATTCGATAGAGACGTTT  >  NC_000913/3705918‑3706116

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: