BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	1,756,553	A→T	100%	K286* (AAA→TAA)	pykF →	pyruvate kinase I

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,756,553	0	A	T	100.0%	337.4 / ‑6.7	97	K286* (AAA→TAA)	pykF	pyruvate kinase I
Reads supporting (aligned to +/- strand): ref base A (0/0); major base T (51/45); minor base G (1/0); total (52/45)

Only 100 of 101 total aligned reads displayed.

GAAGAAGTTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTGAATCCGCAAAAG  >  NC_000913/1756457‑1756649

gaagaaGTTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCtaaa                                                                                               >  1:3105393/1‑100 (MQ=255)

gaagaaGTTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCtaaa                                                                                               >  2:2438520/1‑100 (MQ=255)

gaagaaGTTATCTTCACCCAAAAGATGATGATAGAAAAATGTATCCATACACATAAAATCGAAAACACTACGACCCAGATACAAGATTCAATGATCtaaa                                                                                               >  1:1123740/1‑100 (MQ=255)

   gaagTTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCTAAAAcc                                                                                            <  2:1610907/100‑1 (MQ=255)

    aagTTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCTAAAAccc                                                                                           >  1:1032633/1‑100 (MQ=255)

    aagTTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCTAAAAccc                                                                                           >  1:1335321/1‑100 (MQ=255)

    aagTTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCTAAAAcc                                                                                            >  1:3076156/1‑99 (MQ=255)

     agTTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCTAAAACCCa                                                                                          <  2:1980160/100‑1 (MQ=255)

       ttATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCTAAAACCCACg                                                                                        <  1:1064745/100‑1 (MQ=255)

        tATCTTCGCCCAAAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGaaaaaaaaaaaacaa                                                                                       <  1:234644/100‑8 (MQ=255)

         aTCTTCGCCCAGAAGATGATGATCGACAAATGTATCCGTGCACGTAAAGTCGTTAACACTGCGACCCAGATGCTGGATTCCATGATCTAAAACCCACGcn                                                                                      <  1:1635249/100‑2 (MQ=255)

         aTCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCTAAAACCCACGcc                                                                                      <  2:650040/100‑1 (MQ=255)

          tattCGCCCTTCAGAGGATTATCGAAAAATGTATCCGTGCACGTATAGTCGTTATCACTGCGCCCCAGATGCTGCATGCCATGATCTAAAACCCACGccc                                                                                     <  1:1038010/98‑1 (MQ=255)

          tCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCTAAAACCCACGccc                                                                                     <  2:287156/100‑1 (MQ=255)

           cTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCTAAAACCCACGCCCg                                                                                    >  1:2935139/1‑100 (MQ=255)

              cGCCCAGAAGATGATGATCGAATAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCTAAAACCCACGCCCGACt                                                                                 <  1:233422/100‑1 (MQ=255)

              cGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCTAAAACCCACGCCCGACt                                                                                 <  2:1540047/100‑1 (MQ=255)

              cGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCTAAAACCCACGCCCGACt                                                                                 <  1:1484821/100‑1 (MQ=255)

               gCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATACCATGATCTAAAAACCACGCAAGACt                                                                                 >  1:2756167/1‑99 (MQ=255)

                 ccAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCTAAAACCCACGCCCGACTcgc                                                                              >  2:1845775/1‑100 (MQ=255)

                 ccAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCTAAAACCCACGCCCGACTcgc                                                                              >  2:2808124/1‑100 (MQ=255)

                 ccAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTACGACCCAGATGCTGGATTCCATGATCTAAAACCCACGCCCGACTcgc                                                                              >  1:1537895/1‑100 (MQ=255)

                  cAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCTTTATCACTGCGACCCAGATGCTGGATTCCATGATCTAAAACCCACGCCCGACTcgcg                                                                             <  1:203969/100‑1 (MQ=255)

                  cAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCTAAAACCCACGCCCGACTcgcg                                                                             >  1:1971054/1‑100 (MQ=255)

                  cAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCTAAAACCCACGCCCGACTcgcg                                                                             >  2:252670/1‑100 (MQ=255)

                    gaagaTGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCTAAAACCCACGCCCGACTCGCGCa                                                                           <  2:1819323/100‑1 (MQ=255)

                         tgatgatCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCTAAAACCCACGCCCGACTCGCGCAGAAGc                                                                      >  2:242870/1‑100 (MQ=255)

                            tgctCGAAAAATGTTTCCGTGAACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCTAAAACCCACGCCCGACTCGCGCAGAAGCCgg                                                                   <  2:799413/97‑1 (MQ=255)

                            tgatCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCTAAAACCCACGCCCGACTCGCGCAGAAGCCgg                                                                   <  1:281938/100‑1 (MQ=255)

                            tgatCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCTAAAACCCACGCCCGACTCGCGCAGAAGCCgg                                                                   >  1:3180116/1‑100 (MQ=255)

                            tgatCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCTAAAACCCACGCCCGACTCGCGCAGAAGCCgg                                                                   <  2:1296594/100‑1 (MQ=255)

                              atCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCTAAAACCCACGCCCGACTCGCGCAGAAGCCGGt                                                                  >  1:1631682/1‑99 (MQ=255)

                               tCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCTAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGa                                                                >  2:252838/1‑100 (MQ=255)

                                cGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCTAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGAc                                                               >  2:2454293/1‑100 (MQ=255)

                                 gAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCTAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACg                                                              >  2:2175260/1‑100 (MQ=255)

                                 gAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCTAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACg                                                              >  1:2064613/1‑100 (MQ=255)

                                 gAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCTAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACg                                                              >  1:3081174/1‑100 (MQ=255)

                                  aaaaaTGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCTAAAACCCACGCCCGACTCGCGCAGAAGCCGGTTACGt                                                             >  2:255813/1‑100 (MQ=255)

                                  aaaaaTGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCTAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGt                                                             <  2:2482251/100‑1 (MQ=255)

                                  aaaaaTGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCTAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGt                                                             >  1:741207/1‑100 (MQ=255)

                                   aaaaTGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCTAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGtt                                                            >  2:1083292/1‑100 (MQ=255)

                                    aaaTGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCTAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTg                                                           <  2:3118037/100‑1 (MQ=255)

                                     aaTGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCTAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTg                                                           <  2:468826/99‑1 (MQ=255)

                                      aTGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCTAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCa                                                         <  1:2371402/100‑1 (MQ=255)

                                        gTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCTAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCaaa                                                       >  1:1778768/1‑100 (MQ=255)

                                          aTCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCTAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACg                                                     >  2:440571/1‑100 (MQ=255)

                                             cGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCTAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCa                                                  >  2:3174986/1‑100 (MQ=255)

                                               tGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGGTTCCATGGTCGAAAAGCCACGCCCGACTCGCGCAGAAGCCGGGGACGTTGCAAACGCCagc                                                >  1:2849787/1‑98 (MQ=255)

                                                gCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCTAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATcc                                               >  1:2742179/1‑100 (MQ=255)

                                                 cACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCTAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCt                                              <  1:2271968/100‑1 (MQ=255)

                                                   cGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCTAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCg                                            >  2:1119477/1‑100 (MQ=255)

                                                      aaaGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCTAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACg                                         <  2:2162710/100‑1 (MQ=255)

                                                       aaGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCTAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACgg                                        <  2:1063384/100‑1 (MQ=255)

                                                       aaGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCTAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACgg                                        <  2:2510814/100‑1 (MQ=255)

                                                        aGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCTAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGt                                       >  2:1296940/1‑100 (MQ=255)

                                                         gTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCTAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTa                                      >  2:239216/1‑100 (MQ=255)

                                                          tCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCTAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTAc                                     <  2:1383995/100‑1 (MQ=255)

                                                          tCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCTAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTAc                                     <  2:1657747/100‑1 (MQ=255)

                                                            gTTATCACTGCGACCCAGATGCTGGATTCCATGATCTAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTg                                   >  2:2079782/1‑100 (MQ=255)

                                                              tATCACTGCGACCCAGATGCTGGATTCCATTATCTAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGAc                                 <  1:687190/100‑1 (MQ=255)

                                                              tATCACTGCGACCCAGATGCTGGATTCCATGATCTAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGAc                                 <  1:634196/100‑1 (MQ=255)

                                                              tATCACTGCGACCCAGATGCTGGATTCCATGATCTAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGAc                                 <  2:1113509/100‑1 (MQ=255)

                                                               aTCACTGCGACCCAGATGCTGGATTCCATGATCTAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGACg                                <  1:2044855/100‑1 (MQ=255)

                                                                 cACTGCGACCCAGATGCTGGATTCCATGATCTAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGACGCa                              >  2:3354369/1‑100 (MQ=255)

                                                                 cACTGCGACCCAGATGCTGGATTCCATGATCTAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGACGCa                              <  2:507131/100‑1 (MQ=255)

                                                                   cTGCGACCCAGATGCTGGATTCCATGATCTAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGACGCAGt                            >  2:438187/1‑100 (MQ=255)

                                                                   cTGCGACCCAGATGCTGGATTCCATGATCTAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGACGCAGt                            >  2:2803514/1‑100 (MQ=255)

                                                                    tGCGACCCAGATGCTGGATTCCATGATCTAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGACGCAGTg                           >  2:2026244/1‑100 (MQ=255)

                                                                     gCGACCCAGATGCTGGATTCCATGATCTAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGACGCAGTGa                          <  2:1459126/100‑1 (MQ=255)

                                                                       gACCCAGATGCTGGATTCCATGATCTAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATg                        <  1:1329536/100‑1 (MQ=255)

                                                                       gACCCAGATGCTGGATTCCATGATCTAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATg                        <  2:393008/100‑1 (MQ=255)

                                                                          ccAGATGCTGGATTCCATGATCTAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTg                     >  2:2402364/1‑100 (MQ=255)

                                                                           cAGATGCTGGATTCCATGATCTAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGt                    >  2:1653582/1‑100 (MQ=255)

                                                                           cAGATGCTGGATTCCATGATCTAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGt                    >  2:501933/1‑100 (MQ=255)

                                                                            aGATGCTGGATTCCATGATCTAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTc                   >  1:2184189/1‑100 (MQ=255)

                                                                            aGATGCTGGATTCCATGATCTAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTc                   <  1:1722596/100‑1 (MQ=255)

                                                                              aTGCTGGATTCGATGAGCTAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTc                   <  1:1226838/98‑1 (MQ=255)

                                                                               tGCTGGATTCCATGATCTAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCtag                <  2:2309824/100‑3 (MQ=255)

                                                                               tGCTGGATTCCATGATCTAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTgg                >  2:750464/1‑100 (MQ=255)

                                                                                gCTGGATTCCATGATCTAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGt               <  2:1987504/100‑1 (MQ=255)

                                                                                gCTGGATTCCATGATCTAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGt               >  1:356570/1‑100 (MQ=255)

                                                                                 cTGGATTCCATGATCTAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTg              <  1:1404017/100‑1 (MQ=255)

                                                                                 cTGGATTCCATGATCTAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTg              >  2:632106/1‑100 (MQ=255)

                                                                                 cTGGATTCCATGATCTAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTg              >  1:2976314/1‑100 (MQ=255)

                                                                                 cTGGATTCCATGATCTAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTg              >  2:1308328/1‑100 (MQ=255)

                                                                                 cTGGATTCCATGATCTAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTg              <  1:3200926/100‑1 (MQ=255)

                                                                                 cTGGATTCCATGATCTAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTg              >  2:1052601/1‑100 (MQ=255)

                                                                                 cTGGATTCCATGATCTAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTg              <  2:3294886/100‑1 (MQ=255)

                                                                                       tCCATGATCTAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGCGAATCCg        <  2:3040347/100‑1 (MQ=255)

                                                                                        ccATGATCTAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTGAATCCGc       >  2:3121490/1‑100 (MQ=255)

                                                                                        ccATGATCTAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTGAATCCGc       >  2:1766510/1‑100 (MQ=255)

                                                                                         cATGATCTAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTGAATCCGCa      >  2:2605151/1‑100 (MQ=255)

                                                                                          aTGATCTAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTGAATCCGCa      >  1:472531/1‑99 (MQ=255)

                                                                                          aTGACCTAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGAAGTTGCAACCGCCATCCTCGACGGTACTGCCGCACTGATGCTGCCTGGTGAGTCCGCaa     >  1:931056/1‑100 (MQ=255)

                                                                                           tGATCTAAAATCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTGAATCCGCaaa    <  1:2239402/100‑1 (MQ=255)

                                                                                           tGATCTAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTGAATCCGCaaa    <  1:1565021/100‑1 (MQ=255)

                                                                                           tGATCTAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTGAATCCGCaaa    >  2:147290/1‑100 (MQ=255)

                                                                                            gATCTAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTGAATCCGCaaaa   >  2:3386527/1‑100 (MQ=255)

                                                                                             aTCTAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGTTACTGACGCAGTGATGCTGTCTGGTGAATCCGCAAAAg  <  1:421169/100‑1 (MQ=255)

                                                                                             aTCTAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTGAATCCGCAAAAg  <  1:419672/100‑1 (MQ=255)

GAAGAAGTTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTGAATCCGCAAAAG  >  NC_000913/1756457‑1756649

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`