Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,781,140	0	A	G	24.1%	158.8 / 16.1	86	intergenic (+123/‑75)	lldD/trmL	L‑lactate dehydrogenase, FMN‑linked/tRNA Leu mC34,mU34 2'‑O‑methyltransferase, SAM‑dependent
Reads supporting (aligned to +/- strand):  ref base A (40/23);  new base G (0/20);  total (41/45)
Fisher's exact test for biased strand distribution p-value = 1.34e-07
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTC‑CCTCGCCCCTCC‑GGGGAGAGGGCCGGGG‑TGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACG  >  NC_000913/3781041‑3781236                                                                                                      |                                                                                                  cccTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTC‑CCTCGCCCCTCC‑GGGgaga                                                                                                   >  2:798305/1‑100 (MQ=255)  ccTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTC‑CCTCGCCCCTCC‑GGGGagag                                                                                                  <  1:3043894/100‑1 (MQ=255)  ccTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTC‑CCTCGCCCCTCC‑GGGGagag                                                                                                  <  1:1127620/100‑1 (MQ=255)  ccTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTC‑CCTCGCCCCTCC‑GGGGagag                                                                                                  <  2:600219/100‑1 (MQ=255)  ccTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTC‑CCTCGCCCCTCC‑GGGGagag                                                                                                  >  2:1794515/1‑100 (MQ=255)  ccTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTC‑CCTCGCCCCTCC‑GGGGagag                                                                                                  >  2:2336675/1‑100 (MQ=255)   ctctCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTC‑CCTCGCCCCTCC‑GGGGAGAgg                                                                                                 >  1:969519/1‑100 (MQ=255)   ctctCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTC‑CCTCGCCCCTCC‑GGGGAGAgg                                                                                                 >  2:118298/1‑100 (MQ=255)   ctctCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTC‑CCTCGCCCCTCC‑GGGGAGAgg                                                                                                 >  2:2805960/1‑100 (MQ=255)    tctcCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTC‑CCTCGCCCCTCC‑GGGGAGAggg                                                                                                <  2:2320669/100‑1 (MQ=255)    tctcCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTC‑CCTCGCCCCTCC‑GGGGAGAggg                                                                                                <  2:1871277/100‑1 (MQ=255)       cccTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTC‑CCTCGCCCCTCC‑GGGGAGAGGGCCg                                                                                             >  2:1978694/1‑100 (MQ=255)       cccTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTC‑CCTCGCCCCTCC‑GGGGAGAGGGCCg                                                                                             >  2:611330/1‑100 (MQ=255)         cTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTC‑CCTCGCCCCTCC‑GGGGAGCGGGCCggg                                                                                           >  1:741342/1‑100 (MQ=255)          tgaGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTC‑CCTCGCCCCTCC‑GGGGAGAGGGCCgggg                                                                                          <  2:228382/98‑1 (MQ=255)          tCAGGGAGAGGGGACCTTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTC‑CCTCGCCCCTCC‑GGGGAGAGGGCCgggg                                                                                          <  1:2067165/100‑1 (MQ=255)          tCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTC‑CCTCGCCCCTCC‑GGGGAGAGGGCCgggg                                                                                          <  2:2963885/100‑1 (MQ=255)          tCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTC‑CCTCGCCCCTCC‑GGGGAGAGGGCCgggg                                                                                          <  1:1155033/100‑1 (MQ=255)              ggAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTC‑CCTCGCCCCTCC‑GGGGAGAGGGCCGGGG‑TGa                                                                                      >  1:2229574/1‑99 (MQ=255)               gagaGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTC‑CCTCGCCCCTCC‑GGGGAGAGGGCCGGGG‑TGAgg                                                                                    <  2:664225/100‑1 (MQ=255)               gagaGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTC‑CCTCGCCCCTCC‑GGGGAGAGGGCCGGGG‑TGAgg                                                                                    >  1:3407573/1‑100 (MQ=255)                agagGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTC‑CCTCGCCCCTCC‑GGGGAGAGGGCCGGGG‑TGAggg                                                                                   <  1:2087250/100‑1 (MQ=255)                 gagGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTC‑CCTCGCCCCTCC‑GGGGAGAGGGCCGGGG‑TGAgggg                                                                                  <  2:2799371/100‑1 (MQ=255)                     ggACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTC‑CCTCGCCCCTCC‑GGGGAGAGGGCCGGGG‑TGAGGGGaaaa                                                                              <  2:1049736/100‑1 (MQ=255)                     ggACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTC‑CCTCGCCCCTCC‑GGGGAGAGGGCCGGGG‑TGAGGGGaaaa                                                                              <  1:3356413/100‑1 (MQ=255)                      gACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTC‑CCTCGCCCCTCC‑GGGGAGAGGGCCGGGG‑TGAGGGGAAAAg                                                                             <  2:898882/100‑1 (MQ=255)                      gACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTC‑CCTCGCCCCTCC‑GGGGAGAGGGCCGGGG‑TGAGGGGAAAAg                                                                             <  1:1314495/100‑1 (MQ=255)                       aCCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTC‑CCTCGCCCCTCC‑GGGGAGAGGGCCGGGG‑TGAGGGGAAAAgg                                                                            <  1:2687343/100‑1 (MQ=255)                          gTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTC‑CCTCGCCCCTCC‑GGGGAGAGGGCCGGGG‑TGAGGGGAAAAGGCCg                                                                         >  2:873958/1‑100 (MQ=255)                          gTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTC‑CCTCGCCCCTCC‑GGGGAGAGGGCCGGGG‑TGAGGGGAAAAGGCCg                                                                         >  2:37306/1‑100 (MQ=255)                             cGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTC‑CCTCGCCCCTCC‑GGGGAGAGGGCCGGGG‑TGAGGGGAAAAGGCCGCAc                                                                      >  1:1463864/1‑100 (MQ=255)                                 gcTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTC‑CCTCGCCCCTCC‑GGGGAGAGGGCCGGGG‑TGAGGGGAAAAGGCCGCACTGCt                                                                  >  1:3085973/1‑100 (MQ=255)                                 gcTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTC‑CCTCGCCCCTCC‑GGGGAGAGGGCCGGGG‑TGAGGGGAAAAGGCCGCACTGCt                                                                  >  2:2945583/1‑100 (MQ=255)                                  ntgtatgtaCTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTC‑CCTCGCCCCTCC‑GGGGAGAGGGCCGGGG‑TGAGGGGAAAAGGCCGCACTGCTc                                                                 >  1:258363/2‑100 (MQ=255)                                  cTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTC‑CCTCGCCCCTCC‑GGGGAGAGGGCCGGGG‑TGAGGGGAAAAGGCCGCACTGCTc                                                                 >  1:2561186/1‑100 (MQ=255)                                    gtatgtaCTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTC‑CCTCGCCCCTCC‑GGGGAGAGGGCCGGGG‑TGAGGGGAAAAGGCCGCACTGCTCAc                                                               >  2:2162693/1‑100 (MQ=255)                                     tttgtcCTCCCTCCCCCTGAACCGCCCCCGCCCTTTTTTTTGCCC‑CCCCGCCCCCCG‑GGGGAGGGGGCCGGGG‑GGGGGGGAAAAGGCCGCACTGCTCAcc                                                              <  1:798301/98‑1 (MQ=255)                                      atgtaCTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTC‑CCTCGCCCCTCC‑GGGGAGAGGGCCGGGG‑TGAGGGGAAAAGGCCGCACTGCTCACCa                                                             >  2:1646512/1‑100 (MQ=255)                                          aCTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTC‑CCTCGCCCCTCC‑GGGGAGAGGACCGGGG‑TGAGGGGAAAAGGCCGCACTGCTCACCAAttt                                                         <  1:2885342/100‑1 (MQ=255)                                           ccccctccctCTGAACCGCCACCGCCCTGTTTTTTTCCC‑CCCCGCCCCCCC‑GGGGGGGGGCCCGGGG‑GGAGGGGAAAAGGCCGCACTGCTCACCaa                                                            <  1:3062809/94‑1 (MQ=255)                                           ccccccCACTGGGAAAGGCCCCCGCTCTTTTTTTTTCCC‑CCCCCCCCCCCG‑GGGGGGGGGGCGGGGG‑GGGGGGGAAAAGGCCGCACTGCTCAc                                                               <  2:52617/91‑1 (MQ=255)                                           cTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTC‑CCTCGCCCCTCC‑GGGGAGAGGGCCGGGG‑TGAGGGGAAAAGGCCGCACTGCTCACCAAtttt                                                        >  1:3275173/1‑100 (MQ=255)                                           cTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTC‑CCTCGCCCCTCC‑GGGGAGAGGGCCGGGG‑TGAGGGGAAAAGGCCGCACTGCTCACCAAtttt                                                        >  2:2637044/1‑100 (MQ=255)                                           cTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTC‑CCTCGCCCCTCC‑GGGGAGAGGGCCGGGG‑TGAGGGGAAAAGGCCGCACTGCTCACCAAtttt                                                        >  2:617376/1‑100 (MQ=255)                                             ccctccctCTGAAACGCCCCCGCCCTCTTTTTTTCCC‑CCTCGCCCCCCG‑GGGGAGAGGGCGGGGT‑TGGGGGGAAAAGGCCGCACTGCTCACCAATTTTCt                                                      <  1:2336658/100‑1 (MQ=255)                                             cccTCCTTCTGAAACGACACCGCACTCTTTTTTGCCC‑CCTCGCCCCCCG‑GGGGAGGGGGCGGGGG‑TGGGGGGAAAAGGCCGCACTGCTCACCAATTTTc                                                       <  1:2364840/99‑1 (MQ=255)                                             cccTCACCCCGAACCGCCCCCGCACTCTTTTTTTCCC‑CCGCGCCCCCCG‑GGGGGGGGGGCCGGGG‑GGGGGGGAAAAGGCCGCACTGCTCAc                                                               <  1:2460721/91‑1 (MQ=255)                                              cccccctctAAAACGACCCCCCACTCTTTTTTCCCC‑CCCCGCCCCCCC‑GGGGGGAGGGCGGGGG‑GGGGGGGAAAAGGCCGCACTGCTCACCAATTTTCTg                                                     <  1:201967/95‑1 (MQ=255)                                               cTCACTCTGAAACGACACCGCACTCTTTTTTTCTC‑CCTCGCCCCTCC‑GGGGAGAGGGCCGGGG‑TGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGc                                                    >  1:2028801/1‑100 (MQ=255)                                               cTCACTCTGAAACGACACCGCACTCTTTTTTTCTC‑CCTCGCCCCTCC‑GGGGAGAGGGCCGGGG‑TGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGc                                                    >  1:975399/1‑100 (MQ=255)                                                    ttagAAAAGCCACCGCACCATTTTTTTCTC‑CTTAGCCCCAAA‑GGGGAAAGGGCCGGGT‑AGAGGGAAAAAGGCAGCACTGCTCACCAATTTTCTGCTAt                                                 <  1:2950959/95‑1 (MQ=255)                                                    tctGAAACGACACCGCACTCTTTTTTTCTC‑CCTCGCCCCTCC‑GGGGAGAGGGCCGGGG‑TGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATcc                                               >  2:1614579/1‑100 (MQ=255)                                                     ctGAAACGACACCGCACTCTTTTTTTCTC‑CCTCGCCCCTCC‑GGGGAGAGGGCCGGGG‑TGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCt                                              >  2:2335781/1‑100 (MQ=255)                                                     cggAAACGCCCCCCCCCTTTTTTTTTCTC‑CCCCGCCCCGCG‑GGGGGGGGGGCGGGGG‑TGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATcc                                               <  2:1162239/97‑1 (MQ=255)                                                      tGAAACGACACCGCACTCTTTTTTTCTC‑CCTCGCCCCTCC‑GGGGAGAGGGCCGGGG‑TGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTg                                             >  2:49730/1‑100 (MQ=255)                                                        aaccGACCCCGCACTCTTTTTTTCCC‑CCTCCCCCCTCG‑GGGGAGAGGGCCGGGG‑TGGGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTg                                             <  1:2022987/95‑1 (MQ=37)                                                            gACACCGCACTCTTTTTTTCTC‑CCTCGCCCCTCC‑GGGGAGAGGGCCGGGG‑TGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCg                                       >  1:3246/1‑100 (MQ=255)                                                              ccccGCATTCTTTTTTTCTC‑CCTCGCCCCCCG‑GGGGAGGGGGCGGGGG‑TGGGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCg                                       <  1:971729/96‑1 (MQ=255)                                                               ccccacctttttttttCCC‑CCCCCCCCCCCCGGGGGGGGGGGCGGGGG‑GGAGGGAAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGccccc                                        <  2:671936/88‑1 (MQ=255)                                                               acCGCACTCTTTTTTTCTC‑CCTCGCCCCTCC‑GGGGAGAGGGCCGGGG‑TGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGcgc                                    >  1:2151895/1‑98 (MQ=255)                                                                cccccctttttttttCCC‑CCCCCCCCCCCC‑GGGGAGGGGGCCGGTG‑GGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCAc                                    <  2:2541793/91‑1 (MQ=255)                                                                 ccctctctTTTTTTCTC‑CCTCGCCCCTCC‑GGGGAGTGGCCGGGGG‑TTGGGGGAAAAGGCCGCACTGCTCACCAAGTTTCTGCTATCCTGccccc                                        <  1:2825985/90‑1 (MQ=255)                                                                 cGCACTCTTTTTTTCTC‑CCTCGCCCCTCC‑GGGGAGAGGGCCGGGG‑TGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTa                                  >  1:2837773/1‑100 (MQ=255)                                                                  gCACTCTTTTTTTCTC‑CCTCGCCCCTCC‑GGGGAGAGGGCCGGGG‑TGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTaa                                 >  2:2252348/1‑100 (MQ=255)                                                                     ctctTTTTTTCTCTCCCGGGCCCTCCGGGGGAGGGGGCGGGGG‑GGGGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAg                                <  2:975403/100‑1 (MQ=255)                                                                     ctctTTTTTTCTC‑CCTCGCCCCTCC‑GGGGAGAGGGCCGGGG‑TGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAggg                              >  2:1989083/1‑100 (MQ=255)                                                                     ctctTTTTTTCTC‑CCTCGCCCCCCC‑GGGGAGAGGGCCGGGG‑TGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAggg                              >  2:508962/1‑100 (MQ=255)                                                                       ttttttttCCC‑CTCCGCCCCCCG‑GGGGGGGGGGCGGGGG‑GGGGGGAAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAgggg                             <  2:3073719/98‑1 (MQ=255)                                                                       ctTTTTTTCTC‑CCTCGCCCCTCC‑GGGGAGAGGGCCGGGG‑TGAGGGGAAAAGGCCGCCCTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAgggg                             >  1:1915363/1‑99 (MQ=255)                                                                       ctTTTTTTCTC‑CCTCGCCCCTCC‑GGGGAGAGGGCCGGGG‑TGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAggggg                            >  1:1386193/1‑100 (MQ=255)                                                                        ttttttcCCC‑CCCCCCCCCCCG‑GGGGGGGGGGCGGGGG‑GGGGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGc                           <  2:969523/100‑1 (MQ=255)                                                                          tttttCCC‑CGTCGCCCCTGG‑GGGATGGGGCCCGGGA‑TGGGGGTAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCTTAGTGCGGGGGCAg                         <  2:1762945/100‑1 (MQ=255)                                                                          tttttCCC‑CCTCCCCCCCCG‑GGGGAGGGGGCCGGGG‑GGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAg                         <  2:297941/100‑1 (MQ=255)                                                                          ttttcCTT‑CCTCGGCCCTGC‑CGGGGGGGGGCCGGGGCTGTGGCGCAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGc                           <  2:84898/99‑1 (MQ=255)                                                                          ttttcCCC‑CCCCGCCCCGCC‑GGGGGGGGGGCGGGGG‑GGGGGGAAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAg                         <  1:2636135/100‑1 (MQ=255)                                                                             ttCTC‑CCCCCCCCCTCG‑GGGGGGGGGGCGGGGG‑GGGGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTAt                      <  2:1463870/100‑1 (MQ=255)                                                                               ctc‑CCTCGCCCCTCC‑GGGGAGAGGGCCGGGG‑TGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGc                    >  2:28520/1‑100 (MQ=255)                                                                               cgc‑ccGCGCACCTCC‑GGGGAGTGGGCGGAGG‑AAAGGAGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCACACTAAGGGGGCAGTATg                     <  1:873954/97‑1 (MQ=255)                                                                                 c‑ccTCGCCCCTCC‑GGGGAGAGGGCCGGGG‑TGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTa                  >  1:2324033/1‑100 (MQ=255)                                                                                 c‑ccTCGCCCCCCG‑GGGGGGAGGGCGGGGG‑GGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCt                   <  1:1767331/99‑1 (MQ=255)                                                                                    cTCGCCCCTCC‑GGGGAGAGGGCCGGGG‑TGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTaaa                >  2:507219/1‑100 (MQ=255)                                                                                      cGCCCCTCC‑GGGGAGAGGGCCGGGG‑TGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACa              >  2:3492935/1‑100 (MQ=255)                                                                                        ccccccG‑GGGGGGAGGGCCGGGT‑GGAGGGAAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTaaa                <  1:129342/96‑1 (MQ=255)                                                                                         cccTCC‑GGGGGGGGGGCCGGGG‑TGGGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCg           <  1:1970135/100‑1 (MQ=255)                                                                                          ccTCG‑GGGGAGGGGGCGGGGG‑GGGGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGAGGCAGTATGCTAAACATCGt          <  1:2162679/100‑1 (MQ=255)                                                                                          ccTCC‑GGGGAGAGGGCCGGGG‑TGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGt          >  2:2333824/1‑100 (MQ=255)                                                                                          ccTCC‑GGGGAGAGGGCCGGGG‑TGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGt          >  1:488150/1‑100 (MQ=255)                                                                                           cccggggggggggggCGGGGG‑GGGGGGAAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGt          <  2:3407599/95‑1 (MQ=255)                                                                                           cTCC‑GGGGAGAGGGCCGGGG‑TGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTa         >  1:93931/1‑100 (MQ=255)                                                                                            tCC‑GGGGAGAGGGCCGGGG‑TGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTAc        >  1:1597829/1‑100 (MQ=255)                                                                                             cc‑GGGGGGAGGGCCGGGG‑TGGGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTa         <  1:1646506/98‑1 (MQ=255)                                                                                              cgggggAGGGGCCGGGGG‑GGGGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACt       <  2:1711906/98‑1 (MQ=255)                                                                                                ggggggAGGGCGGGGG‑TGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACttt     <  1:399557/100‑1 (MQ=255)                                                                                                   gagaGGGCCGGGG‑TGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACg  >  2:801571/1‑100 (MQ=255)                                                                                                      |                                                                                                  CCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTC‑CCTCGCCCCTCC‑GGGGAGAGGGCCGGGG‑TGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACG  >  NC_000913/3781041‑3781236

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑