Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,183,345:1 +T 15.4% intergenic (+22/+468) zupT → / ← ribB zinc transporter/3,4‑dihydroxy‑2‑butanone‑4‑phosphate synthase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,183,3451.T15.4% 675.9 / 136.0 201intergenic (+22/+468)zupT/ribBzinc transporter/3,4‑dihydroxy‑2‑butanone‑4‑phosphate synthase
Reads supporting (aligned to +/- strand):  ref base . (98/72);  new base T (19/12);  total (117/84)
Fisher's exact test for biased strand distribution p-value = 8.43e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 211 total aligned reads displayed.

CCCAGCTATGGCGTACTATGTGGAATGTCAGTGATGGGATTCAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTGTTTAAGTATTTGTTCCGCGCTTCAAGC  >  NC_000913/3183246‑3183444
                                                                                                    |                                                                                                   
     cTATGGCGTACTATGTGGAATGTCAGTGATGGGATTCAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCa                                                                                                >  2:3390473/1‑100 (MQ=255)
     cTATGGCGTACTATGTGGAATGTCAGTGATGGGATTCAGTTTAGTGCTGCTACAAACGGCAGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCa                                                                                                >  1:2197949/1‑100 (MQ=255)
      tagggCGTACTATGTGGAATGTCAGTGATGGAATTCAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCAt                                                                                               <  2:474789/97‑1 (MQ=255)
      tATGGCGTACTATGTGGAATGTCAGTGATGGGATTCAGTTTAGTGCTGCTACAAACGGCGGGAATTGGGTAAAAACATCATCAGAAGTTGTCCAGCGCAt                                                                                               >  1:2604737/1‑100 (MQ=255)
            gTACTATGTGGAATGTCAGTGATGGGATTCAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACaa                                                                                         >  2:2889516/1‑100 (MQ=255)
             tACTATGTGGAATGTCAGTGATGGGATTCAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAAc                                                                                        <  2:751969/100‑1 (MQ=255)
              aCTATGTGGAATGTCAGTGATGGGATTCAGTTTAGTGTTGCTACAAACGGCGGAAATCAGAAAAAAACAACATCAGAAGTTGTCCATGCGCTGGACAAc                                                                                        <  1:2102583/99‑1 (MQ=25)
              aCTATGTGGAATGTCAGTGATGGGATTCAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCATGCGCTGGACAACt                                                                                       <  2:1052421/100‑1 (MQ=39)
                tATGTGGAATGTCAGTGATGGGATTCAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCATGCGCTGGACAACtt                                                                                      <  1:3308223/99‑1 (MQ=39)
                tATGTGGAATGTCAGTGATGGGATTCAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCATGCGCTGGACAACTTc                                                                                     <  2:185489/100‑1 (MQ=39)
                tATGTGGAATGTCAGTGATGGGATTCAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTc                                                                                     >  2:1002876/1‑100 (MQ=255)
                 aTGTGGAATGTCAGTGATGGGATTCAGTTTAGTGCTGCTACAAACGGTGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCATGCGCTGGACAACTTCt                                                                                    <  2:131924/100‑1 (MQ=38)
                 aTGTGGAATGTCAGTGATGGGATTCAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCATGCGCTGGACAACTTCt                                                                                    <  2:3280427/100‑1 (MQ=38)
                   gtgGAATTAGAGTGATGGTGGTCAGTCGCGTGCTGTTACCGACGGCGGGAATTGGTTAAAAACAACTGCAGAAGTTCTCTAGCGCATGGACAAGTTccag                                                                                  <  1:1705179/100‑4 (MQ=255)
                   gtgGAATGTCAGTGATGGGATTCAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCATGCGCTGGACAACTTCt                                                                                    <  1:2123793/98‑1 (MQ=38)
                   gtgGAATGTCAGTGATGGGATTCAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGc                                                                                  >  1:466176/1‑100 (MQ=255)
                   gtgGAATGTCAGTGATGGGATTCAGGTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAGGTTGTCCAGCGCATGGACAACTTCTGc                                                                                  <  1:1132041/100‑1 (MQ=255)
                   gtgCAATGTAAGTGATGGGATTCAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCATGCGCTGGACAACTTCTg                                                                                   <  1:2943773/99‑1 (MQ=37)
                     ggAATGTCAGTGATGGGATTCAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCATGCGCTGGACAACTTCTgatg                                                                                <  2:2987449/100‑4 (MQ=38)
                     ggAATGTCAGTGATGGGATTCAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCa                                                                                >  1:3536773/1‑100 (MQ=255)
                     ggAATGTCAGTGATGGGATTCAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCa                                                                                <  2:2052747/100‑1 (MQ=255)
                       aaTGTCAGTGATGGGATTCAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGc                                                                              <  2:260432/100‑1 (MQ=255)
                            cAGTGATGGGATTCAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCAc                                                                         >  1:195580/1‑100 (MQ=255)
                            cAGTGATGGGATTCAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCAc                                                                         >  1:1267218/1‑100 (MQ=255)
                              gTGATGGGATTCAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGa                                                                       <  2:1949777/100‑1 (MQ=255)
                               tGATGGGATTCAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGaa                                                                      <  2:3034631/100‑1 (MQ=255)
                                gATGGGATTCAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGaaa                                                                     >  2:3053694/1‑100 (MQ=255)
                                gATGGGATTCAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGaaa                                                                     >  1:2971574/1‑100 (MQ=255)
                                 aTGGGATTCAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAg                                                                    <  2:1613176/100‑1 (MQ=255)
                                 aTGGGAGTCAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAg                                                                    <  2:2399810/100‑1 (MQ=255)
                                  tGGGATTCAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGc                                                                   >  2:3143430/1‑100 (MQ=255)
                                  tGGGATTCAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGc                                                                   >  1:353016/1‑100 (MQ=255)
                                   gggATTCAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGcc                                                                  <  2:3816713/100‑1 (MQ=255)
                                    ggATTCAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCt                                                                 >  2:2464633/1‑100 (MQ=255)
                                      aTTCAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTa                                                               <  1:2734192/100‑1 (MQ=255)
                                        tcagggtAGTGCGGCGATAAACGGAGGTGTTTGGTTAAAAACAGTATCAGAAGTAGTCCACGGCATGGGCAACTTCTGCCGGCTTCACGAAAGCCTTAtt                                                             <  1:3349851/94‑1 (MQ=255)
                                          aGTTTAGTGCTGCTACAAACGGCGGGCATTGGTTAAACACAACATCAGCAGTTGTCCAGCGCATGGACAGCTTCTGGCAGCTTCACGATAGCCTTAtt                                                             <  1:3744436/98‑1 (MQ=255)
                                          aGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCt                                                           <  1:2434756/100‑1 (MQ=255)
                                            tttAGTGCTGCTACAAACGGCGGGAAGTGGTTAAAAACAACGTCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCCGCTGCACGAAAGCCTTATTCTAc                                                         <  1:3252151/100‑1 (MQ=255)
                                             ttAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTAcc                                                        <  2:3108944/100‑1 (MQ=255)
                                              tAGTGCTGCTACAAACGGCTGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGTACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTAcc                                                        <  2:3642697/99‑1 (MQ=255)
                                               aGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTTACGAAAGCCTTATTCTACCt                                                       <  1:2366040/99‑1 (MQ=255)
                                                  gctgctACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTc                                                   >  1:2413262/1‑100 (MQ=255)
                                                  gctgctACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGAACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTc                                                   >  2:2119693/1‑100 (MQ=255)
                                                    tgctACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCgg                                                 <  2:333815/100‑1 (MQ=255)
                                                     gctACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGc                                                <  2:2312662/100‑1 (MQ=255)
                                                     gctACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGc                                                >  2:409469/1‑100 (MQ=255)
                                                     gctACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGc                                                <  1:247696/100‑1 (MQ=255)
                                                     gctACAAACGGCGGGAATTGGTCAAAAACAACATCAGAAGTTGTACAGCGCATGGACACCTTCTGCCCGCTACACGAAAGCCTTATTCTACCTTTTCGGc                                                <  1:3658552/100‑1 (MQ=255)
                                                      ctACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTCATTCTACCTTTTCGGCt                                               <  1:3037598/100‑1 (MQ=255)
                                                      ctACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTACTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCt                                               >  2:50069/1‑100 (MQ=255)
                                                       tACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTg                                              >  1:1464853/1‑100 (MQ=255)
                                                        aCAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGt                                             >  1:864143/1‑100 (MQ=255)
                                                        aCAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGt                                             >  1:2139807/1‑100 (MQ=255)
                                                         cAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGtc                                            >  2:3518404/1‑100 (MQ=255)
                                                          aaaCGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGtct                                           >  1:3204119/1‑100 (MQ=255)
                                                          aaaCGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGtct                                           >  1:2765134/1‑100 (MQ=255)
                                                             cggcggGAATTGGTTAAAAGCAACATCAGAAGTTGTCCAGCGTATGGACAACTTCTGCCAGCTTCCCGAAAGCCTTATTCTACCTTTTCGGCTGtctct                                         <  1:2225820/99‑1 (MQ=255)
                                                             cggcggGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGtctctt                                        >  2:608220/1‑100 (MQ=255)
                                                                cggGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTTCCTTTTCGGCTGtctcttctc                                     <  1:3074040/100‑1 (MQ=255)
                                                                 gggAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTATACCTTTTCGGCTGtctcttctct                                    >  1:1902922/1‑100 (MQ=255)
                                                                  ggAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTtctctc                                   <  2:667166/100‑1 (MQ=255)
                                                                  ggAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTtctctc                                   >  2:220950/1‑100 (MQ=255)
                                                                   gAATTGGTTAAAAACAACATCAGAAGTTGTCGAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTGGCGGCTGTCTCTTCTCTCg                                  <  1:1586088/100‑1 (MQ=255)
                                                                   gAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCg                                  <  1:1061325/100‑1 (MQ=255)
                                                                   gAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCg                                  >  2:1454439/1‑100 (MQ=255)
                                                                         gTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACt                              >  1:2579057/1‑98 (MQ=255)
                                                                         gTTAAAAACAACATCAGAAATTGTCAAGCGAATGGACAACTTCTGCCAACTTAACGAAAGCATTATTCTACTTTTTAGGCTATCTCTTCTATTGTa                                >  1:365152/1‑96 (MQ=255)
                                                                          ttAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTGtt                           >  2:3289747/1‑100 (MQ=255)
                                                                          ttAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTGtt                           >  2:1188169/1‑100 (MQ=255)
                                                                           tAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTGttt                          >  2:84449/1‑100 (MQ=255)
                                                                           tAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTGttt                          >  1:1022466/1‑100 (MQ=255)
                                                                           tAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTGttt                          >  2:689741/1‑100 (MQ=255)
                                                                            aaaaaCAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTGTTTa                         >  1:3556350/1‑100 (MQ=255)
                                                                              aaaCAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTGTTTAAg                       <  2:3556376/100‑1 (MQ=255)
                                                                                  aacaTCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTGTTTAAGTAtt                   <  2:2991179/100‑1 (MQ=255)
                                                                                  aacaTCAGAAGTTGTCCAGCGCAGGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCGTTTCGGCTGTCTCTTCTCTCGTACTGTTTAAGTAt                    <  2:253682/99‑1 (MQ=255)
                                                                                   acaTCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTGTTTAAGTAt                    <  1:1920742/98‑1 (MQ=255)
                                                                                    caTCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTGTTTAAGTATTTg                 <  1:404459/100‑1 (MQ=255)
                                                                                    caTCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTGTTTAAGTATTTg                 >  2:1677306/1‑100 (MQ=255)
                                                                                    caTCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTGTTTAAGTATTTg                 >  2:792266/1‑100 (MQ=255)
                                                                                     aTCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTGTTTAAGTATTTGt                >  1:2449061/1‑100 (MQ=255)
                                                                                     aTCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTGTTTAAGTATTTGt                >  2:1968704/1‑100 (MQ=255)
                                                                                     aTCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTCCTCTCGTACTGTTTAAGTATTTGt                >  1:342649/1‑100 (MQ=255)
                                                                                       cAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTGTTTAAGTATTTGTTc              >  1:2876207/1‑100 (MQ=255)
                                                                                        aGAAGTTGTCCATGCGCTGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTGTTTAAGTATTTGTTcc             >  2:1357124/1‑100 (MQ=38)
                                                                                         gAAGTTTTCTAGCGCATGGATATCTTTTGTCAGCTTCACGAAAGCCTTATTCTATCTTTTCGGCTGTCTCTTCTCTCGTACTGTTTACGTATTTGtt               <  1:1678144/97‑1 (MQ=255)
                                                                                         gAAGTTGTCCATGCGCTGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTGTTTAAGTATTTGTTCcg            >  1:1410709/1‑100 (MQ=39)
                                                                                         gAAGTTGTCCATGCGCTGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTGTTTAAGTATTTGTTCcg            >  2:891348/1‑100 (MQ=39)
                                                                                         gAAGTTGTCCATGCGCTGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTGTTTAAGTATTTGTTCcg            >  1:284988/1‑100 (MQ=39)
                                                                                         gAAGTTGTCCATGCGCTGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTGTTTAAGTATTTGTTCcg            >  2:26469/1‑100 (MQ=39)
                                                                                         gAAGTTGTCCATGCGCTGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCACTCATACTGTTTAAGTATTTCtt               >  1:1458425/1‑97 (MQ=37)
                                                                                         gAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTGTTTAAGTATTTGTTCcg            >  1:1768226/1‑100 (MQ=255)
                                                                                          aaGTTGTCCATGCGCTGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTGTTTAAGTATTTGTTCcgc           >  1:70939/1‑100 (MQ=39)
                                                                                            gTTGTCCATGCGCTGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTGTTTAAGTATTTGTTCCgcgc         >  2:1211195/1‑100 (MQ=39)
                                                                                             ttGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTGTTTAAGTATTTGTTCCGCGCt        <  1:817332/100‑1 (MQ=255)
                                                                                             ttGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTGTTTAAGTATTTGTTCCGCGCt        <  2:1927584/100‑1 (MQ=255)
                                                                                                 ccAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTGTTTAAGTATTTGTTCCGCGCTTCaa    >  2:1065476/1‑100 (MQ=255)
                                                                                                  cAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTGTTTAAGTATTTGTTCCGCGCTTCAAg   >  2:2246231/1‑100 (MQ=255)
                                                                                                   aGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTGTTTAAGTATTTGTTCCGCGCTTCAAGc  <  2:2176138/100‑1 (MQ=255)
                                                                                                    |                                                                                                   
CCCAGCTATGGCGTACTATGTGGAATGTCAGTGATGGGATTCAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTGTTTAAGTATTTGTTCCGCGCTTCAAGC  >  NC_000913/3183246‑3183444

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: