New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	= 4449524	155 (1.120)	7 (0.050)	5/168	NT	4.4%	coding (129/531 nt)	ppa	inorganic pyrophosphatase
	?	NC_000913	= 4449539	158 (1.210)					coding (114/531 nt)	ppa	inorganic pyrophosphatase
Rejected: Frequency below cutoff threshold.

ACGGAGTTGGGACCAGTACGTCAACCGGGTCACCGTCCAGAGACAGGGTGTGGTTGATGTAACCGTAGTTGCATGGATAGAACATCGCGGTGGACATGAAGCGG‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913/4449421‑4449524 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑agcggCGCACTGTTCGTTGACCGCTTCATGTCCACCGCGATGTTCTATCCATGCAACTACGGTTACATCAACCACACCCTGTCTCTGGACGGTGACCCGGTTGA  <  NC_000913/4449539‑4449441                                                                                                                                                                                                             ACGGAGTTGGGACCAGTACGTCAACCGGGTCACCGTCCAGAGACAGGGTGTGGTTGATGTAACCGTAGTTGCATGGATAGAACATCGCGGTGGACATGAA                                                                                                         <  1:3228720/100‑1  nGGAGTTGGGACCAGTACGTCAACCGGGTCACCGTCCAGAGACAGGGTGTGGTTGATGTAACCGTAGTTGCATGGATAGAACATCGCGGTGGACATGAAG                                                                                                        >  1:2931846/2‑100  CGGAGTTGGGACCAGTACGTCAACCGGGTCACCGTCCAGAGACAGGGTGTGGTTGATGTAACCGTAGTTGCATGGATAGAACATCGCGGTGGACATGAAG                                                                                                        >  2:2909436/1‑100  CGGAGTTGGGACCAGTACGTCAACCGGGTCACCGTCCAGAGACAGGGTGTGGTTGATGTAACCGTAGTTGCATGGATAGAACATCGCGGTGGACATGAAG                                                                                                        >  2:3629263/1‑100  CGGAGTTGGGACCAGTACGTCAACCGGGTCACCGTCCAGAGACAGGGTGTGGTTGATGTAACCGTAGTTGCATGGATAGAACATCGCGGTGGACATGAAG                                                                                                        >  2:1452864/1‑100   GGAGTTGGGACCAGTACGTCAACCGGGTCACCGTCCAGAGACAGGGTGTGGTTGATGTAACCGTAGTTGCATGGATAGAACATCGCGGTGGACATGAAGC                                                                                                       <  1:2191997/100‑1   GGAGTTGGGACCAGTACGTCAACCGGGTCACCGTCCAGAGACAGGGTGTGGTTGATGTAACCGTAGTTGCATGGATAGAACATCGCGGTGGACATGAAGC                                                                                                       <  2:1264665/100‑1   GGAGTTGGGACCAGTACGTCAACCGGGTCACCGTCCAGAGACAGGGTGTGGTTGATGTAACCGTAGTTGCATGGATAGAACATCGCGGTGGACATGAAGC                                                                                                       >  2:486133/1‑100   GGAGTTGGGACCAGTACGTCAACCGGGGCACCGGCCAGAGACAGGGTGTGGTTGATGTAACCGTAGTTGCATGGATAGAACATCGCGGTGGACATGAAGC                                                                                                       <  1:1127759/100‑1     AGTTGGGACCAGTACGTCAACCGGGTCACCGTCCAGAGACAGGGTGTGGTTGATGTAACCGTAGTTGCATGGATAGAACATCGCGGTGGACATGAAGCGG                                                                                                     >  2:704199/1‑100       cgatctCCAGTACGTCAACCGGGTCACCGTCCAGAGACAGGGTGTGGTTGATGTAACCGTAGTTGCATGGATAGAACATCGCGGTGGACATGAAGCGGCc                                                                                                   <  2:2597715/94‑2                             GTCACCGTCCAGAGACAGGGTGTGGTTGATGTAACCGTAGTTGCATGGATAGAACATCGCGGTGGACATGAAGCGGCGCACTGTTCGTTGACCGCTTCAT                                                                             <  1:1425468/100‑1                             GTCACCGTCCAGAGACAGGGTGTGGTTGATGTAACCGTAGTTGCATGGATAGAACATCGCGGTGGACATGAAGCGGCGCACTGTTCGTTGACCGCTTCAT                                                                             <  2:3297219/100‑1                              TCACCGTCCAGAGACAGGGTGTGGTTGATGTAACCGTAGTTGCATGGATAGAACATCGCGGTGGACATGAAGCGGCGCACTGTTCGTTGACCGCTTCATG                                                                            <  1:2419217/100‑1                               CACCGTCCAGAGACAGGGTGTGGTTGATGTAACCGTAGTTGCATGGATAGAACATCGCGGTGGACATGAAGCGGCGCACTGTTCGTTGACCGCTTCATGT                                                                           <  1:2124278/100‑1                                   GTCCAGAGACAGGGTGTGGTTGATGTAACCGGAGTTGCATGGATAGAACATCGCGGTGGACATGAAGCGGGGCACTGTTCGTTGACCGCTTCATG                                                                            <  1:3236883/95‑1                                          GACAGGGTGTGGTTGATGTAACCGTAGTTGCATGGATAGAACATCGCGGTGGACATGAAGCGGCGCACTGTTCGTTGACCGCTTCATGTCCACCGCGATG                                                                >  1:812201/1‑100                                                         ATGTAACCGTAGTTGCATGGATAGAACATCGCGGTGGACATGAAGCGGCGCACTGTTCGTTGACCGCTTCATGTCCACCGCGATGTTCTATCCATGCAAC                                                 <  2:812208/100‑1                                                                                                    AGCGGCGCACTGTTCGTTGACCGCTTCATGTCCACCGCGATGTTCTATCCATGCAACTACGGTTACATCAACCACACCCTGTCTCTGGACGGTGACCCGG      <  2:170022/100‑1                                                                                                    AGCGGCGCACTGTTCGTTGACCGCTTCATGTCCACCGCGATGTTCTATCCATGCAACTACGGTTACATCAACCACACCCTGTCTCTGGACGGTGACCCGG      <  1:2655591/100‑1                                                                                                     GCGGCGCACTGTTCGTTGACCGCTTCATGTCCACCGCGATGTTCTATCCATGCAACTACGGTTACATCAACCACACCCTGTCTCTGGACGGTGACCCGGT     <  2:1415273/100‑1                                                                                                     GCGGCGCACTGTTCGTTGACCGCTTCATGTCCACCGCGATGTTCTATCCATGCAACTACGGTTACATCAACCACACCCTGTCTCTGGACGGTGACCCGGT     <  2:1286645/100‑1                                                                                                       GGCGCACTGTTCGTTGACCGCTTCATGTCCACCGCGATGTTCTATCCATGCAACTACGGTTACATCAACCACACCCTGTCTCTGGACGGTGACCCGGTTG   >  2:467616/1‑100                                                                                                        GCGCACTGTTCGTTGACCGCTTCATGTCCACCGCGATGTTCTATCCATGCAACTACGGTTACATCAACCACACCCTGTCTCTGGACGGTGACCCGGTTGA  >  2:260192/1‑100                                                                                                                                                                                                             ACGGAGTTGGGACCAGTACGTCAACCGGGTCACCGTCCAGAGACAGGGTGTGGTTGATGTAACCGTAGTTGCATGGATAGAACATCGCGGTGGACATGAAGCGG‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913/4449421‑4449524 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑agcggCGCACTGTTCGTTGACCGCTTCATGTCCACCGCGATGTTCTATCCATGCAACTACGGTTACATCAACCACACCCTGTCTCTGGACGGTGACCCGGTTGA  <  NC_000913/4449539‑4449441

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.