Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 83,590 G→T 15.8% intergenic (‑61/+32) leuA ← / ← leuL 2‑isopropylmalate synthase/leu operon leader peptide

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_00091383,5900GT15.8% 423.8 / 66.8 160intergenic (‑61/+32)leuA/leuL2‑isopropylmalate synthase/leu operon leader peptide
Reads supporting (aligned to +/- strand):  ref base G (73/61);  new base T (13/12);  total (87/73)
Fisher's exact test for biased strand distribution p-value = 8.31e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.71e-01

Only 100 of 163 total aligned reads displayed.

GCGCAATGTGGTATCGAAAATAATGACTTGCTGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCCGCTCACTCGTCTACCGCGCAAAGAAGATGCGTTTAGTAGTAGTAGACCGATAA  >  NC_000913/83491‑83689
                                                                                                   |                                                                                                   
gcgcAATGTGGTATCGAAAATAATGACTTGCTGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGAGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATg                                                                                                     >  1:2034430/1‑100 (MQ=255)
 cgcAATGTGGTATCGAAAATAATGACTTGCTGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGTGCAATgg                                                                                                    <  2:2033994/100‑1 (MQ=255)
  gcAATGTGGTATCGAAAATAATGACTTGCTGGCTCATGGTTTGGGTCCTTGTCTATTTTAGAGCGCCTCGATTCGGGCATAAAAAAACCCGCGCAATGgc                                                                                                   >  1:2289641/1‑100 (MQ=255)
    aaTGTGGTATCGAAAATAATGACTTGCTGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGgcgc                                                                                                 <  1:3197362/100‑1 (MQ=255)
     aTGTGGTATCGAAAATAATGACTTGCTGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGcgcg                                                                                                <  2:1223793/100‑1 (MQ=255)
      tgtgGTATCGAAAATAATGACTTGCTGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCgg                                                                                               <  1:1781050/100‑1 (MQ=255)
        tgGTATCGAAAATAATGACTTGCTGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCCATTGCGCGGGt                                                                                             <  2:3324845/100‑1 (MQ=255)
        tgGTATCGAAAATAATGACTTGCTGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGt                                                                                             >  2:129486/1‑100 (MQ=255)
         ggTATCGAAAATAATGACTTGCTGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAGAACCCGCGCAATGGCGCGGGtt                                                                                            >  2:2607710/1‑100 (MQ=255)
         ggTATCGAAAATAATGACTTGCTGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCCATTGCGCGGGtt                                                                                            <  1:529444/100‑1 (MQ=255)
           tATCGAAAATAATGACTTGCTGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCCATTGCGCGGGtttt                                                                                          <  2:2582542/100‑1 (MQ=255)
           tATCGAAAATAATGACTTGCTGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGtttt                                                                                          <  2:1895100/100‑1 (MQ=255)
            aTCGAAAATAATGACTTGCTGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCCATTGCGCGGGttttt                                                                                         <  2:3064058/100‑1 (MQ=255)
            aTCGAAAATAATGACTTGCTGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGttttt                                                                                         >  2:410558/1‑100 (MQ=255)
            aTCGAAAATAATGACTTGCTGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGttttt                                                                                         >  2:548867/1‑100 (MQ=255)
            aTCGAAAATAATGACTTGCTGGATCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGAGGGtt                                                                                            >  1:2274901/1‑97 (MQ=255)
             tCGAAAATAATGACTTGCTGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCCATTGCGCGGGtttttt                                                                                        <  2:2122387/100‑1 (MQ=255)
              cGAAAATAATGACTTGCTGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCCATTGCGCGGGttttttt                                                                                       <  2:3705755/100‑2 (MQ=255)
              cGAAAATAATGACTTGCTGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTtttg                                                                                       <  1:2503846/100‑1 (MQ=255)
              cGAAAATAATGACTTGCTGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTtttg                                                                                       >  2:3446208/1‑100 (MQ=255)
               gAAAATAATGACTTGCTGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTAGGGCATAAAAAAACCCGCGCAATGGCGCGGGtttttt                                                                                        >  1:2273617/1‑98 (MQ=255)
               gAAAATAATGACTTGCTGGCTCATAGTTTGGGTCCTTGACTGTTTTAGAGCTGGTCGTTTCGGTCATAAAAACACCCGCGTCATTGCGCGGGtttttt                                                                                        <  1:2368420/98‑1 (MQ=255)
                 aaaTAATGACTTGCTGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCCATTGCGCGGGtttttttatg                                                                                    <  2:671694/100‑5 (MQ=38)
                  aataatGACTTGCTGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTtttgtttg                                                                                   >  2:1653606/1‑100 (MQ=255)
                  aataatGACTTGCTGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTtttgtttg                                                                                   <  1:1844566/100‑1 (MQ=255)
                  aataatGACTTGCTGGCTCATGGTTTGGGTCATTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAACACAACCCGCGCAATGGCGCGGGTTTtttgtttg                                                                                   <  1:2571537/100‑1 (MQ=255)
                      atGACTTGCTGGCTCATGGGTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTtttgtttg                                                                                   <  1:2577858/96‑1 (MQ=255)
                       tGACTTGCTGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGc                                                                              >  1:314738/1‑100 (MQ=255)
                         aCTTGCTGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGt                                                                            >  2:1022107/1‑100 (MQ=255)
                         aCTTGCTGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGt                                                                            >  1:846163/1‑100 (MQ=255)
                          cTTGCTGGCTCATGGTTTGGGTTCTTGTCTCTTTTAGACCGCCTCGCTTCGGGCATCAAAAAACCCTCGTAATGGCGCGGGTTTCTGCTTTGACTGc                                                                              >  1:1660557/1‑97 (MQ=255)
                          cTTGCTGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTg                                                                           >  2:2813434/1‑100 (MQ=255)
                             gCTGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTg                                                                        >  2:2991581/1‑100 (MQ=255)
                             gCTGGCTCATGGTTTGGGTCCTTGTCTCTCTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCCCGGGTTTCTTGTTTGACTGCGTg                                                                           >  1:308292/1‑97 (MQ=255)
                              cTGGCTGATGGTTTGTGTCCTTGTGACTTTTAGAGCGCCTCGCTTCGGGTATAAAACAATCCGCGCAACGTCTCGTGATTTTTTTTTGACCGCGtctggg                                                                       >  1:376178/1‑95 (MQ=255)
                              cTGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGc                                                                          >  1:3297152/1‑97 (MQ=255)
                              cTGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTgg                                                                       <  2:3240151/100‑1 (MQ=255)
                               tGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGc                                                                      <  2:2144725/100‑1 (MQ=255)
                               tGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGc                                                                      >  2:3558724/1‑100 (MQ=255)
                                 gCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCtt                                                                    <  2:2278991/100‑1 (MQ=255)
                                  cTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTa                                                                   >  1:1504449/1‑100 (MQ=255)
                                   tCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTaa                                                                  <  2:2666026/100‑1 (MQ=255)
                                    cATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAAt                                                                 >  2:2250709/1‑100 (MQ=255)
                                       ggTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCt                                                              <  1:1335727/100‑1 (MQ=255)
                                       ggTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCt                                                              <  2:152326/100‑1 (MQ=255)
                                          ttGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGa                                                           <  1:855346/100‑1 (MQ=255)
                                            gggTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTCATGCTGGATg                                                         >  2:2419665/1‑100 (MQ=255)
                                              gTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGcc                                                       >  1:1477775/1‑100 (MQ=255)
                                                 cTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCCGCt                                                    >  1:3482861/1‑100 (MQ=255)
                                                 cTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCCGCt                                                    >  2:1546528/1‑100 (MQ=255)
                                                    gTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCCGCTCAc                                                 >  2:2291632/1‑100 (MQ=255)
                                                    gTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCCGCTCAc                                                 >  1:3589843/1‑100 (MQ=255)
                                                      ctctTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCCGCTCACTc                                               >  1:698470/1‑100 (MQ=255)
                                                       tctTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCCGCTCACTCg                                              <  1:913460/100‑1 (MQ=255)
                                                         ttttAGAGCGCCCCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGCTTCTTGTCTGACCGCCTACTCGCTTAATGCGCGATGCCGCTCACTCg                                              >  1:1024581/1‑98 (MQ=255)
                                                             agagCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCCGCTCACTCGTCTAcc                                        <  1:765350/100‑1 (MQ=255)
                                                              gagCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCCGCTCACTCGTCTACcg                                       >  2:1463358/1‑100 (MQ=255)
                                                              gagCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCCGCTCACTCGTCTACcg                                       >  1:413039/1‑100 (MQ=255)
                                                                gcgcCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGTCGCTCACTCGTCTACcgcg                                     <  1:145591/100‑1 (MQ=255)
                                                                 cgcCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCCGCTCACTCGTCTACCgcgc                                    <  1:184167/100‑1 (MQ=255)
                                                                   ccTCGCTTCGGGCATAAAAGAACCCGCGCACGCGCGCGGGTCTTTTGTTAGACTCCGAGCTGCCTTAAAGCTGGATGCCGCTCACTCTTCTACCgcgcga                                  >  1:3777870/1‑98 (MQ=255)
                                                                   ccTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCCGCTCACTCGTCTACCGCGCaa                                  >  1:135949/1‑100 (MQ=255)
                                                                    cTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCCGCTCACTCGTCTACCGCGCaaa                                 >  2:90403/1‑100 (MQ=255)
                                                                      cGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCCGCTCACTCGTCTACCGCGCAaaga                               >  1:1359046/1‑100 (MQ=255)
                                                                        cTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCCGCTCACTCGTCTACCGCGCAaagaag                             <  2:1676068/100‑1 (MQ=255)
                                                                        cTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCCGCTCACTCGTCTACCGCGCAaagaag                             >  2:1061095/1‑100 (MQ=255)
                                                                          tCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCCGCTCACTCGTCTACCGCGCAAAGAAGAt                           >  1:828206/1‑100 (MQ=255)
                                                                           cGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCCGCTCACTCGTCTACCGCGCAAAGAAGATg                          >  2:3603131/1‑100 (MQ=255)
                                                                           cGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCCGCTCACTCGTCTACCGCGCAAAGAAGATg                          <  1:296790/100‑1 (MQ=255)
                                                                            gggCATAAAAAGACCCGCGTAGTGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCCGCTCACGCGTCTACCGCGCAAAGAAGATGc                         <  1:3687945/100‑1 (MQ=255)
                                                                             ggCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCCGCTCACTCGTCTACCGCGCAAAGAAGATGCg                        >  2:790230/1‑100 (MQ=255)
                                                                             ggCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCCGCTCACTCGTCTACCGCGCAAAGAAGATGCg                        <  2:1541720/100‑1 (MQ=255)
                                                                             ggCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCCGCTCACTCGTCTACCGCGCAAAGAAGATGCg                        <  2:300628/100‑1 (MQ=255)
                                                                               cATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCCGCTCACTCGTCTACCGCGCAAAGAAGATCCGtt                      >  1:2932133/1‑100 (MQ=255)
                                                                                aacaaaaaaCCCGCGCCATTGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCCGCTCACTCGTCTACCGCGCAAAGAAGATGCGttt                     >  2:1831977/4‑100 (MQ=255)
                                                                                aacaaaaaaCCCGCGCCATTGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCCGCTCACTCGTCTACCGCGCAAAGAAGATGCGttt                     >  1:2041777/4‑100 (MQ=255)
                                                                                aacaaaaaaCCCGCGCCATTGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCCGCTCACTCGTCTACCGCGCAAAGAAGATGCGttt                     >  2:2935288/4‑100 (MQ=38)
                                                                                aTAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTTCGTGCTGGCTTAATGCTGGATGCCGCTCACTCGTCTACCGCGCAAAGAAGATGCGttt                     <  1:1908797/100‑1 (MQ=255)
                                                                                aTAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCCGCTCACTTGTCTACCGCGCAAAGAAGATGCGttt                     <  2:3594994/100‑1 (MQ=255)
                                                                                aTAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCCGCTCACTCGTCTACCGCGCAAAGAAGATGCGttt                     <  2:757065/100‑1 (MQ=255)
                                                                                 tAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCCGCTCACTCGTCTACCGCGCAAAGAAGATGCGTTta                    >  2:2754234/1‑100 (MQ=255)
                                                                                 tAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCCGCTCACTCGTCTACCGCGCAAAGAAGATGCGTTta                    >  1:21185/1‑100 (MQ=255)
                                                                                   aaaaaaCCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCCGCTCACTCGTCTACCGCGCAAagaagagatcggaaga                  >  2:1878544/1‑90 (MQ=255)
                                                                                     aaaaCCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCCGCTCACTCGTCTACCGCGCAAAGAAGATGCGTTtagtag                <  1:1993614/100‑1 (MQ=255)
                                                                                     aaaaCCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCCGCTCACTCGTCTACCGCGCAAAGAAGATGCGTTtagtag                >  2:2915964/1‑100 (MQ=255)
                                                                                       aaCCCGCGCCATTGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCCGCTCACTCGTCTACCGCGCAAAGAAGATGCGTTtagtagta              >  2:2494559/1‑100 (MQ=255)
                                                                                       aaCCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCCGCTCACTCGTCTACCGCGCAAAGAAGATGCGTTtagtagta              <  1:2694282/100‑1 (MQ=255)
                                                                                        aCCCGCGCCATTGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCCGCTCACTCGTCTACCGCGCAAAGAAGATGCGTTtagtagtag             >  2:2061124/1‑100 (MQ=255)
                                                                                        aCCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCCGCTCACTCGTCTACCACGCAAAGAAGATGCGTTtagtagtag             <  2:145296/100‑1 (MQ=255)
                                                                                         cccGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCCGCTCACTCGTCTACCGCGCAAAGAAGATGCGTTtagtagtagt            <  1:2981621/100‑1 (MQ=255)
                                                                                         cccGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCCGCTCACTCGTCTACCGCGCAAAGAAGATGCGTTtagtagtagt            >  2:119604/1‑100 (MQ=255)
                                                                                         cccGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCCGCTCACTCGTCTACCGCGCAAAGAAGATGCGTTtagtagtagt            >  2:2088639/1‑100 (MQ=255)
                                                                                         cccGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCCGCTCACTCGTCTACCGCGCAAAGAAGATGCGTTtagtagtagt            <  2:1392478/100‑1 (MQ=255)
                                                                                          ccGCGCAATGGCGCGGGTTTTTTGTTTTTTTTCTTGCTGGCTTAATGCTGGATGCCGCTCACTCGTCTACCGCGCAAAGAAGATGCGTTtagtagtagta           >  1:2338770/1‑100 (MQ=255)
                                                                                          ccGCGCAATGGCGCGGGTTTTTTGTTTGGCTGCGTGCTGGCTTAATGCTGGATGCCGCTCACTCGTCTACCGCGCAAAGAAGATGCGTTtagtagtag             <  1:1555137/98‑1 (MQ=255)
                                                                                          ccGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCCGCTCACTCGTCTACCGCGCAAAGAAGATGCGTTtagtagtagta           >  2:2435684/1‑100 (MQ=255)
                                                                                           cgcgCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCCGCTCACTCGTCTACCGCGCAAAGAAGATGCGTTtagtagtagtag          <  2:308297/100‑1 (MQ=255)
                                                                                             cgcAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCCGCTCACTCGTCTACCGCGCAAAGAAGATGCGTTTAGTAGTAGTAGAc        <  2:2755855/100‑1 (MQ=255)
                                                                                             cgcAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCCGCTCACTCGTCTACCGCGCAAAGAAGATGCGTTTAGTAGTAGTAGAc        <  1:2916722/100‑1 (MQ=255)
                                                                                             cgcAATGGCGCGGGTTTTTTGTTTGACTGAGTGCTGGATTAATGCTGGATGCCGCTCACTCGTCTACCGCGCAAAGAATATGCGTTTAGTAGTAGTAGAc        <  1:2193411/100‑1 (MQ=255)
                                                                                                   |                                                                                                   
GCGCAATGTGGTATCGAAAATAATGACTTGCTGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCCGCTCACTCGTCTACCGCGCAAAGAAGATGCGTTTAGTAGTAGTAGACCGATAA  >  NC_000913/83491‑83689

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: