BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	3,248,023	G→A	100%	W84* (TGG→TAG)	yqjA →	general envelope maintenance protein; DedA family inner membrane protein; putative multidrug efflux transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,248,023	0	G	A	100.0%	461.8 / NA	132	W84* (TGG→TAG)	yqjA	general envelope maintenance protein; DedA family inner membrane protein; putative multidrug efflux transporter
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (71/61); total (71/61)

Only 100 of 137 total aligned reads displayed.

AGTTTACTGGTATTGGTCGGCGTGTTGATTGCGAAAGGCGCGATGGGCTATCCGCAAACGATTCTGCTGCTGACCGTTGCCGCCAGCCTCGGCTGCTGGGTCAGCTATATTCAGGGGCGATGGCTGGGCAATACCCGCACCGTACAAAACTGGCTATCTCATTTACCCGCGCATTATCATCAACGCGCACACC  >  NC_000913/3247926‑3248118

aGTTTACTGGTATTGGTCGGCGTGTTGATTGCGAAAGGCGCGATGGGCTATCCGCAAACGATTCTGCTGCTGACCGTTGCCGCCAGCCTCGGCTGCTggg                                                                                               <  1:3560228/100‑1 (MQ=255)

aGTTTACTGGTATTGGTCGGCGTGTTGATTGCGAAAGGCGCGATGGGCTATCCGCAAACGATTCTGCTGCTGACCGTTGCCGCCAGCCTCGGCTGCTagg                                                                                               <  2:682727/100‑1 (MQ=255)

 gTTTACTGGTATTGGTCGGCGTGTTGATTGCGAAAGGCGCGATGGGCTATCCGCAAACGATTCTGCTGCTGACCGTTGCCGCCAGCCTCGGCTGCTAGGt                                                                                              >  2:10780/1‑100 (MQ=255)

 gTTTACTGGTATTGGTCGGCGTGTTGATTGCGAAAGGCGCGATGGGCTATCCGCAAACGATTCTGCTGCTGACCGTTGCCGCCAGCCTCGGCTGCTAGGt                                                                                              <  2:2306430/100‑1 (MQ=255)

 gTTTACTGGTATTGGTCGGCGTGTTGATTGCGAAAGGCGCGATGGGCTATCCGCAAACGATTCTGCTGCTGACCGTTGCCGCCAGCCTCGGCTGCTAGGt                                                                                              >  2:3730504/1‑100 (MQ=255)

    tACTGGTATTGGTCGGCGTGTTGATTGCGAAAGGCGCGATGGGCTATCCGCAAACGATTCTGCTGCTGACCGTTGCCGCCAGCCTCGGCTGCTAGGTCAg                                                                                           >  2:2286622/1‑100 (MQ=255)

    tACTGGTATTGGTCGGCGTGTTGATTGCGAAAGGCGCGATGGGCTATCCGCAAACGATTCTGCTGCTGACCGTTGCCGCCAGCCTCGGCTGCTAGGTCAg                                                                                           <  2:1833709/100‑1 (MQ=255)

    tACTGGTATTGGTCGGCGTGTTGATTGCGAAAGGCGCGATGGGCTATCCGCAAACGATTCTGCTGCTGACCGTTGCCGCCAGCCTCGGCTGCTAGGTCAg                                                                                           >  2:2263907/1‑100 (MQ=255)

     aCTGGTATTGGTCGGCGTGTTGATTGCGAAAGGCGCGATGGGCTATCCGCAAACGATTCTGCTGCTGACCGTTGCCGCCAGCCTCGGCTGCTAGGTCAGc                                                                                          <  2:2380964/100‑1 (MQ=255)

      cTGGTATTGGTCGGCGTGTTGATTGCGAAAGGCGCGATGGGCTATCCGCAAACGATTCTGCTGCTGACCGTTGCCGCCAGCCTCGGCTGCTAGGTCAGCt                                                                                         <  2:1398553/100‑1 (MQ=255)

      cTGGTATTGGTCGGCGTGTTGATTGCGAAAGGCGCGATGGGCTATCCGCAAACGATTCTGCTGCTGACCGTTGCCGCCAGCCTCGGCTGCTAGGTCAGCt                                                                                         >  2:493492/1‑100 (MQ=255)

       tGGTATTGGTCGGCGTGTTGATTGCGAAAGGCGCGATGGGCTATCCGCAAACGATTCTGCTGCTGACCGTTGCCGCCAGCCTCGGCTGCTAGGTCAGCta                                                                                        <  2:3377429/100‑1 (MQ=255)

       tGGTATTGGTCGGCGTGTTGATTGCGAAAGGCGCGATGGGCTATCCGCAAACGATTCTGCTGCTGACCGTTGCCGCCAGCCTCGGCTGCTAGGTCAGCta                                                                                        >  1:2362403/1‑100 (MQ=255)

        ggTATTGGTCGGCGTGTTGATTGCGAAAGGCGCGATGGGCTATCCGCAAACGATTCTGCTGCTGACCGTTGCCGCCAGCCTCGGCTGCTAGGTCAGCtat                                                                                       >  1:1203334/1‑100 (MQ=255)

         gTATTGGTCGGCGTGTTGATTGCGAAAGGCGCGATGGGCTATCCGCAAACGATTCTGCTGCTGACCGTTGCCGCCAGCCTCGGCTGCTAGGTCAGCtata                                                                                      >  1:2005317/1‑100 (MQ=255)

         gTATTGGTCGGCGTGTTGATTGCGAAAGGCGCGATGGGCTATCCGCAAACGATTCTGCTGCTGACCGTCGCCGCCAGACTCGGCTGCTAGGTCAGCtata                                                                                      >  1:2605707/1‑100 (MQ=255)

          tATTGGTCGGCGTGTTGATTGCGAAAGGCGCGATGGGCTATCCGCAAACGATTCTGCTGCTGACCGTTGCCGCCAGCCTCGGCTGCTAGGTCAGCTatat                                                                                     <  1:1586682/100‑1 (MQ=255)

             tGGTCGGCGTGTTGATTGCGAAAGGCGCGATGGGCTATCCGCAAACGATTCTGCTGCTGACCGTTGCCGCCAGCCTCGGCTGCTAGGTCAGCTATATTCa                                                                                  >  2:329237/1‑100 (MQ=255)

              ngTCGGCGTGTTGATTGCGAAAGGCGCGATGGGCTATCCGCAAACGATTCTGCTGCTGACCGTTGCCGCCAGCCTCGGCTGCTAGGTCAGCTATATTCAg                                                                                 >  2:2568559/2‑100 (MQ=255)

              ggTCGGCGTGTTGATTGCGAAAGGCGCGATGGGCTATCCGCAAACGATTCTGCTGCTGACCGTTGCCGCCAGCCTCGGCTGCTAGGTCAGCTATATTCAg                                                                                 >  2:2078760/1‑100 (MQ=255)

               gTCGGCGTGTTGATTGCGAAAGGCGCGATGGGCTATCCGCAAACGATTCTGCTGCTGACCGTTGCCGCCAGCCTCGGCTGCTAGGTCAGCTATATTCAgg                                                                                <  2:883121/100‑1 (MQ=255)

               gTCGGCGTGTTGATTGCGAAAGGCGCGATGGGCTATCCGCAAACGATTCTGCTGCTGACCGTTGCCGCCAGCCTCGGCTGCTAGGTCAGCTATATTCAgg                                                                                <  1:1202730/100‑1 (MQ=255)

                tCGGCGTGTTGATTGCGAAAGGCGCGATGGGCTATCCGCAAACGATTCTGCTGCTGACCGTTGCCGCCAGCCTCGGCTGCTAGGTCAGCTATATTCAggg                                                                               <  2:551348/100‑1 (MQ=255)

                 cGGCGTGTTGATTGCGAAAGGCGCGATGGGCTATCCGCAAACGATTCTGCTGCTGACCGTTGCCGCCAGCCTCGGCTGCTAGGTCAGCTATATTCAgggg                                                                              >  2:1710995/1‑100 (MQ=255)

                      tgtTGATTGCGAAAGGCGCGATGGGCTATCCGCAAACGATTCTGCTGCTGACCGTTGCCGCCAGCCTCGGCTGCTAGGTCAGCTATATTCAGGGGCGATg                                                                         >  1:969844/1‑100 (MQ=255)

                       gtTGATTGCGAAAGGCGCGATGGGCTATCCGCAAACGATTCTGCTGCTGACCGTTGCCGCCAGCCTCGGCTGCTAGGTCAGCTATATTCAGGGGCGATgg                                                                        >  1:2969635/1‑100 (MQ=255)

                       gtTGATTGCGAAAGGCGCGATGGGCTATCCGCAAACGATTCTGCTGCTGACCGTTGCCGCCAGCCTCGGCTGCTAGGTCAGCTATATTCAGGGGCGATgg                                                                        >  2:1167126/1‑100 (MQ=255)

                       gtTGATTGCGAAAGGCGCGATGGGCTATCCGCAAACGATTCTGCTGCTGACCGTTGCCGCCAGCCTCGGCTGCTAGGTCAGCTATATTCAGGGGCGATgg                                                                        >  1:50251/1‑100 (MQ=255)

                       gtTGATTGCGAAAGGCGCGATGGGCTATCCGCAAACGATTCTGCTGCTGACCGTTGCAGCCAGCCTCGGCTGCTAGGTCAGCTATATTCAGGGGCGATgg                                                                        >  2:1330697/1‑100 (MQ=255)

                        ttGATTGCGAAAGGCGCGATGGGCTATCCGCAAACGATTCTGCTGCTGACCGTTGCCGCCAGCCTCGGCTGCTAGGTCAGCTATATTCAGGGGCGATGGc                                                                       <  2:1195679/100‑1 (MQ=255)

                         tGATTGCGAAAGGCGCGATGGGCTATCCGCAAACGATTCTGCTGCTGACCGTTGCCGCCAGCCTCGGCTGCTAGGTCAGCTATATTCAGGGGCGATGGCt                                                                      >  2:93851/1‑100 (MQ=255)

                         tGATTGCGAAAGGCGCGATGGGCTATCCGCAAACGATTCTGCTGCTGACCGTTGCCGCCAGCCTCGGCTGCTAGGTCAGCTATATTCAGGGGCGATGGCt                                                                      >  2:1379260/1‑100 (MQ=255)

                         tGATTGCGAAAGGCGCGATGGGCTATCCGCAAACGATTCTGCTGCTGACCGTTGCCGCCAGCCTCGGCTGCTAGGTCAGCTATATTCAGGGGCGATGGCt                                                                      >  1:412563/1‑100 (MQ=255)

                         tGATTGCGAAAGGCGCGATGGGCTATCCGCAAACGATTCTGCTGCTGACCGTTGCCGCCAGCCTCGGCTGCTAGGTCAGCTATATTCAGGGGCGATGGCt                                                                      >  2:3747910/1‑100 (MQ=255)

                            ttGCGAAAGGCGCGATGGGCTATCCGCAAACGATTCTGCTGCTGACCGTTGCCGCCAGCCTCGGCTGCTAGGTCAGCTATATTCAGGGGCGATGGCTggg                                                                   <  1:1442168/100‑1 (MQ=255)

                            ttGCGAAAGGCGCGATGGGCTATCCGCAAACGATTCTGCTGCTGACCGTTGCCGCCAGCCTCGGCTGCTAGGTCAGCTATATTCAGGGGCGATGGCTggg                                                                   >  2:773228/1‑100 (MQ=255)

                            ttGCGAAAGGCGCGATGGGCTATCCGCAAACGATTCTGCTGCTGACCGTTGCCGCCAGCCTCGGCTGCTAGGTCAGCTATATTCAGGGGCGATGGCTggg                                                                   <  1:1666229/100‑1 (MQ=255)

                             tGCGAAAGGCGCGATGGGCTATCCGCAAACGATTCTGCTGCTGACCGTTGCCGCCAGCCTCGGCTGCTAGGTCAGCTATATTCAGGGGCGATGGCTGGGc                                                                  <  1:778646/100‑1 (MQ=255)

                              gCGAAAGGCGCGATGGGCTATCCGCAAACGATTCTGCTGCTGACCGTTGCCGCCAGCCTCGGCTGCTAGGTCAGCTATATTCAGGGGCGATGGCTGGGCa                                                                 >  2:3133477/1‑100 (MQ=255)

                               cGAAAGGCGCGATGGGCTATCCGCAAACGATTCTGCTGCTGACCGTTGCCGCCAGCCTCGGCTGCTAGGTCAGCTATATTCAGGGGCGATGGCTGGGCaa                                                                <  2:1849808/100‑1 (MQ=255)

                               cGAAAGGCGCGATGGGCTATCCGCAAACGATTCTGCTGCTGACCGTTGCCGCCAGCCTCGGCTGCTAGGTCAGCTATATTCAGGGGCGATGGCTGGGCaa                                                                >  2:1522254/1‑100 (MQ=255)

                               cGAAAGGCGCGATGGGCTATCCGCAAACGATTCTGCTGCTGACCGTTGCCGCCAGCCTCGGCTGCTAGGTCAGCTATATTCAGGGGCGATGGCTGGGCaa                                                                >  2:2731685/1‑100 (MQ=255)

                               cGAAAGGCGCGATGGGCTATCCGCAAACGATTCTGCTGCTGACCGTTGCCGCCAGCCTCGGCTGCTAGGTCAGCTATATTCAGGGGCGATGGCTGGGCaa                                                                >  2:665495/1‑100 (MQ=255)

                               cGAAAGGCGCGATGGGCTATCCGCAAACGATTCTGCTGCTGACCGTTGCCGCCAGCCTCGGCTGCTAGGTCAGCTATATTCAGGGGCGATGGCTGGGCaa                                                                >  1:3798056/1‑100 (MQ=255)

                                gAAAGGCGCGATGGGCTATCCGCAAACGATTCTGCTGCTGACCGTTGCCGCCAGCCTCGGCTGCTAGGTCAGCTATATTCAGGGGCGATGGCTGGGCAAt                                                               <  1:1486522/100‑1 (MQ=255)

                                  aaGGTGCGATGGGCTATCCGCAAACGATTCTGCTGCTGACCGTTGCCGCCAGCCCCGGCTGCTAGGTCAGCTGTATTCAGTCGCGATGGCCGGGCAATAc                                                             >  1:1870773/1‑100 (MQ=255)

                                    ggCGCGATGGGCTATCCGCAAACGATTCTGCTGCTGACCGTTGCCGCCAGCCTCGGCTGCTAGGTCAGCTATATTCAGGGGCGATGGCTGGGCAATAccc                                                           <  2:2806642/100‑1 (MQ=255)

                                      cgcgATGGGCTATCCGCAAACGATTCTGCTGCTGACCGTTGCCGCCAGCCTCGGCTGCTAGGTCAGCTATATTCAGGGGCGTTGGCTGGGCAATACCCGc                                                         <  1:37614/100‑1 (MQ=255)

                                      cgcgATGGGCTAACCGCAAACGATTCTCCTGCTCAACGTAGCCGCCAGCCTCGGCTCCTAGGTCAGCTATATTCCGGGGCCATCGCTGGGCAATACCCg                                                          <  1:3267297/99‑1 (MQ=255)

                                       gcgATGGGCTATCCGCAAACGATTCTGCTGCTGACCGTTGCCGCCAGCCTCGGCTGCTAGGTCAGCTATATTCAGGGGCGATGGCTGGGCAATACCCGCa                                                        >  2:2703412/1‑100 (MQ=255)

                                        cgATGGGCTATCCGCAAACGATTCTGCTGCTGACCGTTGCCGCCAGCCTCGGCTGCTAGGTCAGCTATATTCAGGGGCGATGGCTGGGCAATACCCGCAc                                                       <  1:1522241/100‑1 (MQ=255)

                                        cgATGGGCTATCCGCAAACGATTCTGCTGCTGACCGTTGCCGCCAGCCTCGGCTGCTAGGTCAGCTATATTCAGGGGCGATGGCTGGGCAATACCCGCAc                                                       <  1:2731664/100‑1 (MQ=255)

                                          aTGGGCTATCCGCAAACGATTCTGCTGCTGACCGTTGCCGCCAGCCTCGGCTGCTAGGTCAGCTATATTCAGGGGCGATGGCTGGGCAATACCCGCACCg                                                     <  1:1780793/100‑1 (MQ=255)

                                           tGGGCTATCCGCAAACGATTCTGCTGCTGACCGTTGCCGCCAGCCTCGGCTGCTAGGTCAGCTATATTCAGGGGCGATGGCTGGGCAATACCCGCACCGt                                                    >  1:2914583/1‑100 (MQ=255)

                                           tGGGCTATCCGCAAACGATTCTGCTGCTGACCGTTGCCGCCAGCCTCGGCTGCTAGGTCAGCTATATTCAGGGGCGATGGCTGGGCAATACCCGCACCGt                                                    >  2:1634997/1‑100 (MQ=255)

                                            gggCTATCCGCAAACGATTCTGCTGCTGACCGTTGCCGCCAGCCTCGGCTGCTAGGTCAGCTATATTCAGGGGCGATGGCTGGGCAATACCCGCACCGTa                                                   >  1:3186741/1‑100 (MQ=255)

                                            gggCTATCCGCAAACGATTCTGCTGCTGACCGTTGCCGCCAGCCTCGGCTGCTAGGTCAGCTATATTCAGGGGCGATGGCTGGGCAATACCCGCACCGTa                                                   >  1:1842260/1‑100 (MQ=255)

                                             ggCAATCAGCAAACGATCCTGCTGCTGACCGTTGCCGCCAGCCTCGGCTGCTAGGTCAGCTATATTCAGGGGCGATGGCTGGGCAATACCCGCACCGTa                                                   <  2:3611914/99‑1 (MQ=255)

                                              gCTATCCGCAAACGATTCTGCTGCTGACCGTTGCCGCCAGCCTCGGCTGCTAGGTCAGCTATATTCAGGGGCGATGGCTGGGCAATACCCGCACCGTACa                                                 >  2:1066825/1‑100 (MQ=255)

                                              gCTATCCGCAAACGATTCTGCTGCTGACCGTTGCCGCCAGCCTCGGCTGCTAGGTCAGCTATATTCAGGGGCGATGGCTGGGCAATACCCGCACCGTACa                                                 >  2:1810778/1‑100 (MQ=255)

                                                tATCCGCAAACGATTCTGCTGCTGACCGTTGCCGCCAGCCTCGGCTGCTAGGTCAGCTATATTCAGGGGCGATGGCTGGGCAATACCCGCACCGTACaaa                                               >  2:3636755/1‑100 (MQ=255)

                                                tATCCGCAAACGATTCTGCTGCTGACCGTTGCCGCCAGCCTCGGCTGCTAGGTCAGCTATATTCAGGGGCGATGGCTGGGCAATACCCGCACCGTACaaa                                               >  2:1961928/1‑100 (MQ=255)

                                                    cGCAAACGATTCTGCTGCTGACCGTTGCCGCCAGCCTCGGCTGCTAGGTCCGCTATATTCAGGGGCGATGGCTGGGCAATACCCGCACCGTACAAAACTg                                           <  1:236157/100‑1 (MQ=255)

                                                    cGCAAACGATTCTGCTGCTGACCGTTGCCGCCAGCCTCGGCTGCTAGGTCAGCTATATTCAGGGGCGATGGCTGGGCAATACCCGCACCGTACAAAACTg                                           >  1:3229613/1‑100 (MQ=255)

                                                    cGCAAACGATTCTGCTGCTGACCGTTGCCGCCAGCCTCGGCTGCTAGGTCAGCTATATTCAGGGGCGATGGCTGGGCAATACCCGCACCGTACAAAACTg                                           <  1:1507634/100‑1 (MQ=255)

                                                     gCAAACGATTCTGCTGCTGACCGTTGCCGCCAGCCTCGGCTGCTAGGTCAGCTATATTCAGGGGCGATGGCTGGGCAATACCCGCACCGTACAAAACTgg                                          <  1:1855285/100‑1 (MQ=255)

                                                     gCAAACGATTCTGCTGCTGACCGTTGCCGCCAGCCTCGGCTGCTAGGTCAGCTATATTCAGGGGCGATGGCTGGGCAATACCCGCACCGTACAAAACTgg                                          <  1:660560/100‑1 (MQ=255)

                                                     gCAAACGATTCTGCTGCTGACCGTTGCCGCCAGCCTCGGCTGCTAGGTCAGCTATATTCAGGGGCGATGGCTGGGCAATACCCGCACCGTACAAAACTgg                                          >  1:408017/1‑100 (MQ=255)

                                                       aaaCGATTCTGCTGCTGACCGTTGCCGCCAGCCTCGGCTGCTAGGTCAGCTATATTCAGGGGCGATGGCTGGGCAATACCCGCACCGTACAAAACTGGCt                                        <  1:3162329/100‑1 (MQ=255)

                                                        aaCGATTCTGCTGCTGACCGTTGCCGCCAGCCTCGGCTGCTAGGTCAGCTATATTCAGGGGCGATGGCTGGGCAATACCCGCACCGTACAAAACTGGCTa                                       >  2:2441416/1‑100 (MQ=255)

                                                        aaCGATTCTGCTGCTGACCGTTGCCGCCAGCCTCGGCTGCTAGGTCAGCTATATTCAGGGGCGATGGCTGGGCAATACCCGCACCGTACAAAACTGGCTa                                       <  2:1412713/100‑1 (MQ=255)

                                                        aaCGATTCTGCTGCTGACCGTTGCCGCCAGCCTCGGCTGCTAGGTCAGCTATATTCAGGGGCGATGGCTGGGCAATACCCGCACCGTACAAAACTGGCTa                                       <  2:1943511/100‑1 (MQ=255)

                                                          cGATTCTGCTGCTGACCGTTGCCGCCAGCCTCGGCTGCTAGGTCAGCTATATTCAGGGGCGATGGCTGGGCAATACCCGCACCGTACAAAACTGGCTAtc                                     >  1:2196776/1‑100 (MQ=255)

                                                           gATTCTGCTGCTGACCGTTGCCGCCAGCCTCGGCTGCTAGGTCAGCTATATTCAGGGGCGATGGCTGGGCAATACCCGCACCGTACAAAACTGGCTAtct                                    <  1:524626/100‑1 (MQ=255)

                                                           gATTCTGCTGCTGACCGTTGCCGCCAGCCTCGGCTGCTAGGTCAGCTATATTCAGGGGCGATGGCTGGGCAATACCCGCACCGTACAAAACTGGCTAtct                                    <  2:3219893/100‑1 (MQ=255)

                                                             ttCTGCTGCTGACCGTTGCCGCCAGCCTCGGCTGCTAGGTCAGCTATATTCAGGGGCGATGGCTGGGCAATACCCGCACCGTACAAAACTGGCTATCTCa                                  <  1:3848367/100‑1 (MQ=255)

                                                             ttCTGCTGCTGACCGTTGCCGCCAGCCTCGGCTGCTAGGTCAGCTATATTCAGGGGCGATGGCTGGGCAATACCCGCACCGTACAAAACTGGCTATCTCa                                  <  1:1165830/100‑1 (MQ=255)

                                                              tCTGCTGCTGACCGTTGCCGCCAGCCTCGGCTGCTAGGTCAGCTATATTCAGGGGCGATGGCTGGGCAATACCCGCACCGTACAAAACTGGCTATCTCAt                                 <  1:3558474/100‑1 (MQ=255)

                                                               ctgctgctgACCGTTGCCGCCAGCCTCGGCTGCTAGGTCAGCTATATTCAGGGGCGATGGCTGGGCAATACCCGCACCGTACAAAACTGGCTATCTCAtt                                >  2:1574109/1‑100 (MQ=255)

                                                               ctgctgctgACCGTTGCCGCCAGCCTCGGCTGCTAGGTCAGCTATATTCAGGGGCGATGGCTGGGCAATACCCGCACCGTACAAAACTGGCTATCTCAtt                                >  2:345076/1‑100 (MQ=255)

                                                                 gctgctgACCGTTGCCGCCAGCCTCGGCTGCTAGGTCAGCTATATTCAGGGGCGATGGCTGGGCAATACCCGCACCGTACAACACTGGCTATCTCATTTa                              >  1:549001/1‑100 (MQ=255)

                                                                   tgctgACCGTTGCCGCCAGCCTCGGCTGCTAGGTCAGCTATATTCAGGGGCGATGGCTGGGCAATACCCGCACCGTACAAAACTGGCTATCTCATTTAcc                            <  1:773221/100‑1 (MQ=255)

                                                                   tgctgACCGTTGCCGCCAGCCTCGGCTGCTAGGTCAGCTATATTCAGGGGCGATGGCTGGGCAATACCCGCACCGTACAAAACTGGCTATCTCATTTAcc                            <  2:1841079/100‑1 (MQ=255)

                                                                       gACCGTTGCCGCCAGCCTCGGCTGCTAGGTCAGCTATATTCAGGGGCGATGGCTGGGCAATACCCGCACCGTACAAAACTGGCTATCTCATTTACCcgcg                        >  2:1104897/1‑100 (MQ=255)

                                                                         ccGTTGCCGCCAGCCTCGGCTGCTAGGTCAGCTATATTCAGGGGCGATGGCTGGGCAATACCCGCACCGTACAAAACTGGCTATCTCATTTACCCGCGCa                      <  2:3798082/100‑1 (MQ=255)

                                                                         ccGTTGCCGCCAGCCTCGGCTGCTAGGTCAGCTATATTCAGGGGCGATGGCTGGGCAATACCCGCACCGTACAAAACTGGCTATCTCATTTACCCGCGCa                      <  2:2487000/100‑1 (MQ=255)

                                                                         ccGTTGCCGCCAGCCTCGGCTGCTAGGTCAGCTATAATAAGGGGCGATGGCTGGGCAATACCCGCACCGTACAACACTGGCTATCTCATTTACCCGCGCa                      >  1:857889/1‑100 (MQ=255)

                                                                          cGTTGCCGCCAGCCTCGGCTGCTAGGTCAGCTATATTCAGGGGCGATGGCTGGGCAATACCCGCACCGTACAAAACTGGCTATCTCATTTACCCGCGCAt                     <  1:2755244/100‑1 (MQ=255)

                                                                            ttGCCGCCAGCCTCGGCTGCTAGGTCAGCTATATTCAGGGGCGATGGCTGGGCAATACCCGCACCGTACAAAACTGGCTATCTCATTTACCCGCGCATTa                   <  1:3344095/100‑1 (MQ=255)

                                                                            ttGCCGCCAGCCTCGGCTGCTAGGTCAGCTATATTCAGGGGCGATGGCTGGGCAATACCCGCACCGTACAAAACTGGCTATCTCATTTACCCGCGCATTa                   <  2:2337621/100‑1 (MQ=255)

                                                                             tGCCGCCAGCCTCGGCTGCTAGGTCAGCTATATTCAGGGGCGATGGCTGGGCAATACCCGCACCGTACAAAACTGGCTATCTCATTTACCCGCGCATTat                  >  2:2273788/1‑100 (MQ=255)

                                                                               ccgccAGCCTCGGCTGCTAGGTCAGCTATATTCAGGGGCGATGGCTGGGCAATACCCGCACCGTACAAAACTGGCTATCTCATTTACCCGCGCATTatca                >  2:3748101/1‑100 (MQ=255)

                                                                               ccgccAGCCTCGGCTGCTAGGTCAGCTATATTCAGGGGCGATGGCTGGGCAATACCCGCACCGTACAAAACTGGCTATCTCATTTACCCGCGCATTatca                <  2:2973059/100‑1 (MQ=255)

                                                                               ccgccAGCCTCGGCTGCTAGGTCAGCTATATTCAGGGGCGATGGCTGGGCAATACCCGCACCGTACAAAACTGGCTATCTCATTTACCCGCGCATTatca                <  2:3340067/100‑1 (MQ=255)

                                                                                 gccAGCCTCGGCTGCTAGGTCAGCTATATTCAGGGGCGATGGCTGGGCAATACCCGCACCGTACAAAACTGGCTATCTCATTTACCCGCGCATTatcatc              >  1:3033919/1‑100 (MQ=255)

                                                                                     gCCTCGGCTGCTAGGTCAGCTATATTCAGGGGCGATGGCTGGGCAATACCCGCACCGTACAAAACTGGCTATCTCATTTACCCGCGCATTATCATCAAcg          >  2:3388215/1‑100 (MQ=255)

                                                                                       cTCGGCTGCTAGGTCAGCTATATTCAGGGGCGATGGCTGGGCAATACCCGCACCGTACAAAACTGGCTATCTCATTTACCCGCGCATTATCATCAAcgcg        >  1:2261544/1‑100 (MQ=255)

                                                                                          ggCTGCTAGGTCAGCTATATTCAGGGGCGATGGCTGGGCAATACCCGCACCGTACAAAACTGGCTATCTCATTTACCCGCGCATTATCATCAACGCGcac     >  2:2559180/1‑100 (MQ=255)

                                                                                          ggCTGCTAGGTCAGCTATATTCAGGGGCGATGGCTGGGCAATACCCGCACCGTACAAAACTGGCTATCTCATTTACCCGCGCATTATCATCAACGCGcac     >  1:2153900/1‑100 (MQ=255)

                                                                                             tgctaGGTCAGCTATATTCAGGGGCGATGGCTGGGCAATACCCGCACCGTACAAAACTGGCTATCTCATTTACCCGCGCATTATCATCAACGCGCACAcc  <  1:3747671/100‑1 (MQ=255)

AGTTTACTGGTATTGGTCGGCGTGTTGATTGCGAAAGGCGCGATGGGCTATCCGCAAACGATTCTGCTGCTGACCGTTGCCGCCAGCCTCGGCTGCTGGGTCAGCTATATTCAGGGGCGATGGCTGGGCAATACCCGCACCGTACAAAACTGGCTATCTCATTTACCCGCGCATTATCATCAACGCGCACACC  >  NC_000913/3247926‑3248118

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`