Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,705,970 G→A 21.7% intergenic (‑180/+128) dppB ← / ← dppA dipeptide/heme ABC transporter permease/dipeptide/heme ABC transporter periplasmic binding protein; dipeptide chemotaxis receptor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,705,9700GA21.7% 418.2 / 102.1 184intergenic (‑180/+128)dppB/dppAdipeptide/heme ABC transporter permease/dipeptide/heme ABC transporter periplasmic binding protein; dipeptide chemotaxis receptor
Reads supporting (aligned to +/- strand):  ref base G (66/78);  new base A (35/5);  total (101/83)
Fisher's exact test for biased strand distribution p-value = 1.57e-06
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.73e-02

Only 100 of 199 total aligned reads displayed.

CAGGGAATGAAGTATTGCTGCCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCC  >  NC_000913/3705871‑3706069
                                                                                                   |                                                                                                        
cAGGGAATGAAGTATTGCTGCCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATg                                                                                                          >  2:3831259/1‑100 (MQ=255)
cAGGGAATGAAGTATTGCTGCCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATg                                                                                                          >  2:3082477/1‑100 (MQ=255)
  gggAATGAAGTATTGCTGCCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATgcg                                                                                                        >  2:1200743/1‑100 (MQ=255)
  gggAATGAAGTATTGCTGCCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATgcg                                                                                                        >  1:2746110/1‑100 (MQ=255)
  gggAATGAAGTATTGCTGCCGGATCGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATgc                                                                                                         >  1:1809143/1‑99 (MQ=255)
   ggAATGAGGTATGGCTGCCGGATGGCGCTGGAGGCAGAGGCACAGCGCCACCGGGTATACAGATGGTTGACAAAGTGCGCTTTGTTTATGCCGGATgcgg                                                                                                       <  1:3768821/100‑1 (MQ=37)
   ggAATGAAGTATTGCTGCCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATgcgg                                                                                                       >  2:3714066/1‑100 (MQ=255)
    gAATGAAGTATTGCTGCCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATgcggc                                                                                                      <  1:877258/100‑1 (MQ=255)
        gAAGTATTGCTGCCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTa                                                                                                  >  2:1041541/1‑100 (MQ=255)
         aaGTATTGCTGCCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTaa                                                                                                 >  2:2519822/1‑100 (MQ=255)
                gctgcCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTa                                                                                          <  2:909148/100‑1 (MQ=255)
                 ctgcCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTAt                                                                                         <  2:1429576/100‑1 (MQ=255)
                  tgcCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATc                                                                                        >  1:2010273/1‑100 (MQ=255)
                      ggATGGCGCTGCACGCAAAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGAGGCGGCGTTAACGCATTATCCGGc                                                                                    >  1:342366/1‑100 (MQ=255)
                       gATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGcc                                                                                   <  1:282890/100‑1 (MQ=255)
                       gATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGcc                                                                                   <  1:3470915/100‑1 (MQ=255)
                        aTGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCt                                                                                  <  1:3693956/100‑1 (MQ=255)
                        aTGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATAAGGCGTTAACGCCGCATCCGGcat                                                                                  <  2:2060781/100‑3 (MQ=37)
                           gcgcTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACa                                                                              >  1:2980986/1‑100 (MQ=255)
                             gcTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACaaa                                                                            <  2:3028758/100‑1 (MQ=255)
                               tGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAAt                                                                          >  2:3562546/1‑100 (MQ=255)
                                 cACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTAGAAAATCg                                                                        <  2:2023992/100‑1 (MQ=255)
                                 cACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCg                                                                        <  2:488648/100‑1 (MQ=255)
                                 cACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCg                                                                        <  2:937797/100‑1 (MQ=255)
                                 cACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCg                                                                        >  1:1866644/1‑100 (MQ=255)
                                   cGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTg                                                                      >  2:452989/1‑100 (MQ=255)
                                   cGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCGTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCAGGCCTACAAAATCGTg                                                                      >  2:292574/1‑100 (MQ=255)
                                    gatctggCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGc                                                                     <  1:100918/95‑1 (MQ=255)
                                    gCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGc                                                                     <  2:1625288/100‑1 (MQ=255)
                                      agagGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCaa                                                                   >  1:1928904/1‑100 (MQ=255)
                                        agGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAAt                                                                 <  2:428649/100‑1 (MQ=255)
                                         ggCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAAtt                                                                >  1:1961133/1‑100 (MQ=255)
                                          gCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGGTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTc                                                               <  1:696068/100‑1 (MQ=255)
                                           cacaGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCa                                                              >  1:1468190/1‑100 (MQ=255)
                                           cacaGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCa                                                              >  1:620944/1‑100 (MQ=255)
                                             caGTGCAACCGGGTATACAGATTGTAGACAACGTTCGCTTTGTTTGTGGCGTTTGCGGCGTTAACGCCTAATCCGGCTTACAAAATCGTGCAAATTCAAt                                                            <  1:1203186/100‑1 (MQ=17)
                                             caGCGCCACTGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGGCGTATCCCGCCTACAAAATCGTGCAAATTCaa                                                             <  1:525261/99‑1 (MQ=17)
                                             caGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAAt                                                            <  2:3623243/100‑1 (MQ=255)
                                                cgcCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACa                                                         >  1:3186095/1‑100 (MQ=255)
                                                   cACCGGGTATACAGATTGTTGACAAAATGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCTGACCTACAAGATCGTGCAAATTCCATAAAtt                                                       >  1:665051/1‑99 (MQ=17)
                                                    aCCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGc                                                     >  2:1252860/1‑100 (MQ=255)
                                                    aCCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGc                                                     <  1:2357485/100‑1 (MQ=255)
                                                    aCCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGc                                                     <  2:444333/100‑1 (MQ=255)
                                                     ccGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCa                                                    <  2:819002/100‑1 (MQ=255)
                                                     ccGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCa                                                    <  2:3728558/100‑1 (MQ=255)
                                                      cGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAg                                                   <  2:689259/100‑1 (MQ=255)
                                                      cGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAg                                                   >  2:1595785/1‑100 (MQ=255)
                                                          tataCAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAAtt                                             >  1:2698589/1‑100 (MQ=255)
                                                           ataCAGATTGTTGCACAAGTGCTCTGTGTTTATTCCGTATGCGGCGTTTACGCCGTATCCTGCCTACAAAATCGTGAAAATTCAATGCATTGCAGa                                                  <  1:3735098/96‑1 (MQ=12)
                                                             aCAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTc                                          >  1:1134631/1‑100 (MQ=255)
                                                              cAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGTATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTc                                          <  2:734669/99‑1 (MQ=255)
                                                               aGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTC‑‑Gt                                      <  1:3673704/100‑1 (MQ=255)
                                                               aGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTC‑‑Gt                                      >  1:238974/1‑100 (MQ=255)
                                                                cggcataaaCAAAGCGCACTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTC‑‑GTa                                     >  1:2533426/9‑100 (MQ=17)
                                                                 aTTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTC‑‑GTAg                                    <  1:2145517/100‑1 (MQ=255)
                                                                    gttgACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTC‑‑GTAGGcc                                 <  1:2423470/100‑1 (MQ=255)
                                                                     ttgACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTC‑‑GTAGGCCt                                <  2:2715887/100‑1 (MQ=255)
                                                                      tgACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTC‑‑GTAGGCCTg                               >  2:1555601/1‑100 (MQ=255)
                                                                      tgACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTC‑‑GTAGGCCTg                               <  2:757567/100‑1 (MQ=255)
                                                                       gACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTC‑‑GTAGGCCTGa                              >  2:765150/1‑100 (MQ=255)
                                                                        aCAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTC‑‑GTAGGCCTGAt                             >  2:1519232/1‑100 (MQ=255)
                                                                        aCAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTC‑‑GTAGGCCTGAt                             >  1:2834820/1‑100 (MQ=255)
                                                                        aCAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTACAGAATTTTC‑‑GTAGGCCTGAt                             <  2:1123638/100‑1 (MQ=255)
                                                                         cAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTC‑‑GTAGGCCTGATa                            <  1:423645/100‑1 (MQ=255)
                                                                          aaaGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTC‑‑GTAGGCCTGATaa                           <  2:1208219/100‑1 (MQ=255)
                                                                              tGCGCTTTGTTTATGCCGGAGGCGGCGTTACCGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTC‑‑GTAGGCCTGATAAGCgg                       <  2:3364961/100‑1 (MQ=255)
                                                                                 gcTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTC‑‑GTAGGCCTGATAAGCGGAgc                    <  1:1257887/100‑1 (MQ=255)
                                                                                 gcTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTC‑‑GTAGGCCTGATAAGCGGAgc                    <  2:211256/100‑1 (MQ=255)
                                                                                 gcTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTC‑‑GTAGGCCTGATAAGCGGAgc                    <  1:1041533/100‑1 (MQ=255)
                                                                                  cTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTC‑‑GTAGGCCTGATAAGCGGAgcg                   >  2:3765299/1‑100 (MQ=255)
                                                                                  cTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTC‑‑GTAGGCCTGATAAGCGGAgcg                   >  2:3190479/1‑100 (MQ=255)
                                                                                     tttgtaggCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAAACTTTGCAAATTCAATATATTGC‑‑AATCTCCGTGTAGGCCTGATAAGCGTAGCGCAt                >  2:2814988/8‑100 (MQ=18)
                                                                                     tttgtaggCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAAAACATGCAAATTCAATAGATTGCAGAGATTAT‑‑GTAGGCCTGATAAGCGTAGCGCAt                >  2:297290/8‑100 (MQ=17)
                                                                                       tttATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTC‑‑GTAGGCCTGATAAGCGGAGCGCATCa              <  2:238978/100‑1 (MQ=255)
                                                                                       tttATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTC‑‑GTAGGCCTGATAAGCGGAGCGCATCa              <  2:2686460/100‑1 (MQ=255)
                                                                                       tctaggCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAATCATGCAAATCCAAAATATTGCAGGAGTTAT‑‑GTAGGCCTGATAAGCGTAGCGCATCAg             <  1:2876362/95‑1 (MQ=21)
                                                                                        ttATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAg             >  2:3411144/1‑100 (MQ=255)
                                                                                        gtaggCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAAAATCTTGCCAATTCAATATATTGCAGGACCAT‑‑GTAGGCCTGATAAGCGTAGCGCATCAg             >  1:2946257/5‑100 (MQ=18)
                                                                                        gtaggCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAAAGCACGCAAACTCAATATATTGCAGAGATCAT‑‑GTAGGCCTGATAAGCGTAGCGCATCAg             >  2:1787920/5‑100 (MQ=17)
                                                                                        gtaggCCGGATAAGGCGTTCACGCCGCATCCGGCATACAAAAACCTGCAAATTCAATATATTGCAGGATACGT‑‑GTAGGCCTGATAAGCGTAGCGCATCAg             >  2:3044992/5‑100 (MQ=18)
                                                                                         tATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAgg            >  2:3227214/1‑100 (MQ=255)
                                                                                         tATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAgg            >  2:2254131/1‑100 (MQ=255)
                                                                                          aggCCGGATAAGGCGTTTACGCCGCATCCGGCCTACAAATTCTGCAAATTCAACAAATTGCGAATCCCTT‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCa          >  1:3093945/3‑100 (MQ=18)
                                                                                          aggCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAGTTCGTGCAAATTCAATAAATTGCAATATGAC‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGcg          >  2:3844775/3‑99 (MQ=18)
                                                                                          aggCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAAACCTTGCTAATTCAATATATTGCAGGGACTAT‑‑GTAGGCCTGATAAGCATAGCGCATCAGGc           >  1:3522269/3‑100 (MQ=18)
                                                                                          aggCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAATCATGCAAATCCAAAATATTGCAGGAGTTAT‑‑GTAGGCCTGATAAGCGTAGCGCATCAgaga          >  2:2876384/3‑97 (MQ=21)
                                                                                          aggCCGGATAAGGCGTTCACGCCGCATCCGGCATACAAAATTGTGTAAATTCAATAAATTGATCGATCCAC‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGc           >  2:3519958/3‑100 (MQ=21)
                                                                                           ggCCGGATAAGGCGTTTACGCCGCATCCGGCCTACAAAATCGTGCTAATTCAATATATTGCAGAAACCTT‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCa          >  2:3286393/2‑100 (MQ=17)
                                                                                           ggCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAAAATCTTGCCAATTCAATATATTGCAGGACCAT‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCa          >  2:52625/2‑100 (MQ=18)
                                                                                           ggCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAAAGCATGAAAATTCAATATATTGCAGGAGCTGC‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCa          >  1:951293/2‑100 (MQ=17)
                                                                                            gCCGGATAAGGCGTTTACGCCGCATCCGGCCTACAAATTCTGCAAATTCAACAAATTGCGAATCCCTT‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCAtt        >  2:1277516/1‑100 (MQ=18)
                                                                                            gCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAAAATCTTGCCAATTCAATATATTGCAGGACCAT‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCag         >  2:614621/1‑99 (MQ=18)
                                                                                            gCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAAGTCGTATAAATTCAATGTATTGCAGTTTATTG‑‑GTAGGCCTGATAAGCGCAGCGCATCAGGCaa         >  1:998545/1‑99 (MQ=21)
                                                                                             ccGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCAtt        >  2:1906813/1‑100 (MQ=255)
                                                                                              cGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCAttt       >  2:2374711/1‑100 (MQ=255)
                                                                                               ggATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCAtttt      >  1:1435319/1‑100 (MQ=255)
                                                                                                 aTGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTtttg    <  1:436968/100‑1 (MQ=255)
                                                                                                   gcggcgTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTC‑‑GTAGGCCTGATACGCGGAGCGCATCAGCCAttttttc   >  2:663766/1‑97 (MQ=255)
                                                                                                   gcggcgTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTtttgcc  >  1:2210269/1‑100 (MQ=255)
                                                                                                   gcggcgTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTtttgcc  <  1:763975/100‑1 (MQ=255)
                                                                                                   |                                                                                                        
CAGGGAATGAAGTATTGCTGCCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCC  >  NC_000913/3705871‑3706069

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: