Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,060,261 G→T 15.3% intergenic (+31/‑186) typA → / → yihL GTP‑binding protein/putative DNA‑binding transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,060,2610GT15.3% 443.1 / 60.9 164intergenic (+31/‑186)typA/yihLGTP‑binding protein/putative DNA‑binding transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base G (65/73);  new base T (10/15);  total (75/89)
Fisher's exact test for biased strand distribution p-value = 6.63e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.06e-01

Only 100 of 166 total aligned reads displayed.

CGTATTCGTAAACGTCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGGTTTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGCTTTGTCACAATT  >  NC_000913/4060162‑4060359
                                                                                                   |                                                                                                    
ngTATTCGTAAACGTCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAg                                                                                                      >  2:3451465/2‑100 (MQ=255)
  tATTCGTAAACGTCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCg                                                                                                    >  2:2219601/1‑100 (MQ=255)
    ttCGTAAACGTCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGAt                                                                                                  <  1:3034214/100‑1 (MQ=255)
    ttCGTAAACGTCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGAt                                                                                                  <  1:1476774/100‑1 (MQ=255)
      cGTAAACGTCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATgg                                                                                                >  2:3685237/1‑100 (MQ=255)
       gTAAACGTCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGc                                                                                               <  2:345087/100‑1 (MQ=255)
       gTAAACGTCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGc                                                                                               >  2:2574562/1‑100 (MQ=255)
       gTAAACGTCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGc                                                                                               <  1:1438462/100‑1 (MQ=255)
        tAAACGTCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCa                                                                                              >  2:2119378/1‑100 (MQ=255)
         aaaCGTCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAg                                                                                             <  2:2624899/100‑1 (MQ=255)
         aaaCGTCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAg                                                                                             <  1:254784/100‑1 (MQ=255)
          aaCGTCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCATCGCTGGCAgg                                                                                            <  2:2175228/100‑1 (MQ=255)
          aaCGTCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCATCGCTGGCAgg                                                                                            <  2:2174652/100‑1 (MQ=255)
          aaCGTCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCATCGCTGGCAgg                                                                                            <  2:2151735/100‑1 (MQ=255)
          aaCGTCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCATCGCTGGCAgg                                                                                            <  2:1799082/100‑1 (MQ=255)
            cGTCACCTGACGGAAAACGATAGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTAATAAAAAACCTGCCAGCGATGGCAGCtt                                                                                         >  2:163058/1‑100 (MQ=255)
             gTCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGGttt                                                                                        <  2:2124445/100‑1 (MQ=255)
             gTCACCTGACGGAAAACGATCGTCACCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCATCGCTGGCAGGttt                                                                                        <  2:2974000/100‑1 (MQ=39)
               cACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGGttttt                                                                                      >  1:3581742/1‑100 (MQ=255)
                aCCTGACGGAAAACGATCGTAGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCATCGCTGGCAGGtttttt                                                                                    <  1:261316/100‑1 (MQ=255)
                 ccTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGGttttttt                                                                                   >  1:3350381/1‑100 (MQ=255)
                 ccTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGACAGGttttttt                                                                                   >  1:617158/1‑100 (MQ=255)
                   tGACGGAAAACGATCGTCTCCTCTCTAATCGCGCACCGACAGACGATTAATTTTGTTTTTTAGTCATAAAAAACCTGACATCGCTGGCAGGtttttta                                                                                   <  1:451365/98‑2 (MQ=25)
                    gACGGAAACCGATCGTCGCCGCGCCAAACGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCATCGCTGGCAGGttttgtatga                                                                                 <  1:1382773/100‑7 (MQ=37)
                    gACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCATCGCTGGCAGGttttttatga                                                                                <  1:54057/100‑5 (MQ=255)
                     aCGGAAAACGATTGTCGTCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGGTTTTTTTTTGc                                                                               <  1:228179/100‑1 (MQ=255)
                     aCGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGGTTTTTTTTTGc                                                                               <  1:3550267/100‑1 (MQ=255)
                     aCGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGGTTTTTTTTTGc                                                                               <  2:2593623/100‑1 (MQ=255)
                      cGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGGTTTTTTTTTGcc                                                                              >  2:3623557/1‑100 (MQ=255)
                      cGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGGTTTTTTTTTGcc                                                                              >  2:531858/1‑100 (MQ=255)
                       ggAAAACGATCGTCGCCGCGCCAACCGCGCACCGGAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGGtttttttttttgc                                                                             <  2:3245478/100‑5 (MQ=255)
                       ggAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAATACCTGCCAGCGATGGCAGGTTTTTTTTTGCCt                                                                             <  2:3267011/100‑1 (MQ=255)
                        gAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGGTTTTTTTTTGCCTg                                                                            >  2:1536767/1‑100 (MQ=255)
                         aaaaCGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGGTTTTTTTTTGCCTGt                                                                           >  2:3566473/1‑100 (MQ=255)
                          aaaCGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGGtttttttttttct                                                                             >  1:3488194/1‑93 (MQ=255)
                           aaCGATCTTCGCCGCGCCAACCGCGAACCGAAAGACTATTAATTTCGTTTTTTAGTCTTAAAAAACCTGCCAGCGATGGCAGGTTTTTTTTTGCCTGTCg                                                                         <  1:1704605/100‑1 (MQ=255)
                             cGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTCATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGGTTTTTTTTTGCCTGTCGTg                                                                       >  1:2369780/1‑100 (MQ=255)
                             cGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGGTTTTTTTTTGCCTGTCGTg                                                                       >  2:1803142/1‑100 (MQ=255)
                               atcggggccggGCCAACCGCGCATCGAAAGACGAGTAGTTGCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGGTTTTTTTTTGCCTGTCGTGAg                                                                     <  1:767756/94‑1 (MQ=255)
                               aTCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGGTTTTTTTTTGCCTGTCGTGAg                                                                     <  2:1785400/100‑1 (MQ=255)
                                tcgtcgCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGGTTTTTTTTTGCCTGTCGTGAgc                                                                    <  2:1081981/100‑1 (MQ=255)
                                 cgtcgCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGGTTTTTTTTTGCCTGTCGTg                                                                       >  1:1147203/1‑96 (MQ=255)
                                 cgtcgCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGGTTTTTTTTTGCCTGTCGTGAgcg                                                                   <  1:2088142/100‑1 (MQ=255)
                                  gtcgCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGGTTTTTTTTTGCCTGTCGTGAgcgc                                                                  <  2:2737151/100‑1 (MQ=255)
                                  gtcgCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGGTTTTTTTTTGCCTGTCGTGAgcgc                                                                  >  2:2801421/1‑100 (MQ=255)
                                    cgccgcGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGGTTTTTTTTTGCCTGTCGTGAGCGcac                                                                >  2:1724925/1‑100 (MQ=255)
                                      cgacacctaCCGCGCAACGGAAGACGATTGATTTCGTTTTTTAGTCAGAAAAAACCTGCCACCTATGGCACGTTTTTTTTTGCCTGTCCTGAGCCcac                                                                <  1:2181876/90‑1 (MQ=255)
                                       cgcgCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGGTTTTTTTTTGCCTGTCGTGAGCGCACAcc                                                             <  2:3371123/100‑1 (MQ=255)
                                       cgcgCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGGTTTTTTTTTGCCTGTCGTGAGCGCACAcc                                                             <  2:288938/100‑1 (MQ=255)
                                       cgcgCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGGTTTTTTTTTGCCTGTCGTGAGCGCACAcc                                                             <  2:1239733/100‑1 (MQ=255)
                                       cgcgCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGGTTTTTTTTTGCCTGTCGTGAGCGCACAcc                                                             <  1:999053/100‑1 (MQ=255)
                                       cgcgCCAACCGCGCACCGAAAGACGATCAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGGTTTTTTTTTGCCTGTCGTGAGCGCACAcc                                                             <  2:579002/100‑1 (MQ=255)
                                         cgcCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGGTTTTTTTTTGCCTGTCGTGAGCGCACACCaa                                                           >  2:3149002/1‑100 (MQ=255)
                                         cgcCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGGTTTTTTTTTGCCTGTCGTGAGCGCACACCaa                                                           >  2:1990884/1‑100 (MQ=255)
                                           ccACCCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGGTTTTTTTTTGCCTGTCGTGAGCGCACCCCAATg                                                         <  2:2373278/100‑1 (MQ=255)
                                             aaCCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGGTTTTTTTTTGCCTGTCGTGAGCGCACACCAATGCg                                                       <  1:185647/100‑1 (MQ=255)
                                               ccGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGGTTTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAg                                                     >  2:430282/1‑100 (MQ=255)
                                                cgcgCACCGAAAGACGATTAATTTCGTTTTTTAGTCATCAATAACCTGCCAGCGATGGCAGGTTTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAg                                                     <  1:2708589/99‑1 (MQ=255)
                                                 ccgatctgAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGGTTTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTa                                                   <  1:240854/93‑1 (MQ=255)
                                                      ccGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGGTTTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTcc                                              <  2:2126231/100‑1 (MQ=255)
                                                       caaaaGACGAACAACTTCCTTTTTTAGTCATAAAATACCTTCCATCGATTACCGTATTGTTTTTCCCTGTCGTCATCGCACACCAATTCTATTAtctc                                               <  1:2979546/96‑1 (MQ=255)
                                                       cGAAAGACGATTAATTTCGTTTTTTTGTCATAAAAAACCTGCCAGCGATGGGAGGTGTTTGTTTGCCTGTCGTGTGCGCAGACCAATGCGAGTAtc                                                 <  1:769252/96‑1 (MQ=255)
                                                       cGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGGTTTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTccc                                             >  2:309193/1‑100 (MQ=255)
                                                       cGAAAAACGATTAATTTCTTTTTTTATTCATAAAAAAACTGCCAGCGATGGCAGGTTTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTcc                                              <  1:2277544/99‑1 (MQ=255)
                                                        gAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGGTTTTTTTTTTTCTTTTGTTATCGCAAAACAATTCGAGTATCTCCCt                                            >  1:662451/1‑100 (MQ=255)
                                                        gAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGGTTTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCt                                            >  2:3124672/1‑100 (MQ=255)
                                                           aGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGGTTTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTtat                                         <  1:2134280/100‑1 (MQ=255)
                                                            gACGATTAATTTCGTTTTTTAGTCATAAAAGCCCTGCCAGCGATGGCAGGTTTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTtata                                        <  1:1385640/100‑1 (MQ=255)
                                                            gACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGGCAGCGATGGCAGGTTTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCCCCCTtata                                        <  1:1566917/100‑1 (MQ=255)
                                                            gACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGGTTTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTtata                                        <  2:2369797/100‑1 (MQ=255)
                                                            gACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGGTTTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTtata                                        <  1:42610/100‑1 (MQ=255)
                                                                   aaTTTCGTTTTTTAGTCTTAAAAAACCTGCCAGCGATGGCAGGTTTTTTTTTTTCTGTCGTAAGCGCAAACCAATGCGTGCATCTCTCTTATAAGt                                     >  1:2516816/1‑96 (MQ=255)
                                                                         gTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGGTTTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTAc                           <  1:2766136/100‑1 (MQ=255)
                                                                         gTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGGTTTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTAc                           >  2:1408804/1‑100 (MQ=255)
                                                                         gTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGGTTTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTAc                           >  1:412315/1‑100 (MQ=255)
                                                                         gTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGGTTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACg                          <  2:510613/100‑1 (MQ=255)
                                                                          ttttttAGTAATAAAAACCTTGCCAGCAATGGAAGGTTTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTa                            <  2:662458/98‑1 (MQ=255)
                                                                           tttttAGTCATAAAAAACCTGCCAGCGATGGCAGGTTTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGt                         >  2:415526/1‑100 (MQ=255)
                                                                            ttttAGTCATAAAAAACCTGCCAGCGATGGCAGGTTTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTc                        <  1:824863/100‑1 (MQ=255)
                                                                               tAGTCATAAAAAACCTGCCAGCGATGGCAGGTTTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAAt                     <  2:110944/100‑1 (MQ=255)
                                                                               tAGTCATAAAAAACCTGCCAGCGATGGCAGGTTTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAAt                     >  1:1008611/1‑100 (MQ=255)
                                                                               tAGTCATAAAAAACCTGCCAGCGATGGCAGGTTTTTTTTTGCCTGTCGTGAGCGCACACCAATGAGAGTATCTCCCTTATAAGTCTGTGGTTAAGTCAAt                     >  1:2327964/1‑100 (MQ=255)
                                                                                ggcaaaaaaaaaCCTGCCGTCGCTGGCAGGTTTTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGTGTATCTCCCTTATACGTCTCTGATTACGTCAAt                     >  1:1056549/7‑100 (MQ=21)
                                                                                aGTCATAAAAAACCTGCCAGCGATGGCAGGTTTTTCTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAAt                     <  2:1165851/100‑1 (MQ=255)
                                                                                 gTCATAAAAAACCTGCCAGCGATGGCAGGTTTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATag                   <  2:351575/100‑1 (MQ=255)
                                                                                  tCATAAAAAACCTGCCAGGCATGGCAGGGTTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATaga                  <  1:2642415/100‑1 (MQ=255)
                                                                                    aaaaaaaaCCTGCCATCGCTGGCAGGTTTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATagaga                >  2:1864917/3‑100 (MQ=255)
                                                                                    aTAAAAAACCTGCCAGCGATGGCAGGTTTTTTTTTGCCTGTCGTGAGCGCAAACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTCCGTCAATcgag                 >  1:2257983/1‑99 (MQ=255)
                                                                                     tAAAAAACCTGCCAGCGATGGCAGGTTTTTTTTTTTTTTTTTTTAGCGCACACCAATGCGAGTATTTCCCTTTTTAGTCTGTGGTTTCGTCAATag                   >  1:242052/1‑96 (MQ=255)
                                                                                     tAAAAAACCTGCCAGCGATGGCAGGTTTTTTTTTGCATGTCGTGAGAGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATaga                  >  1:1369381/1‑97 (MQ=255)
                                                                                     aaaaaaaCCTGCCATCGCTGGCAGGTTTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATagagag               >  2:3542139/2‑100 (MQ=255)
                                                                                     aaaaaaaCCTGCCATCGCTGGCAGGTTTTTTTTTGCCTGTAGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATagagag               >  2:856738/2‑100 (MQ=255)
                                                                                        aaaaCCTGCCATCGCTGGCAGGTTTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGCtt            >  2:3841479/1‑100 (MQ=255)
                                                                                        aaaaCCTGCCATCGCTGGCAGGTTTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGCtt            >  2:1988515/1‑100 (MQ=255)
                                                                                        aaaaCCTGCCAGCGATGGCAGGTTTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGCtt            <  1:1292716/100‑1 (MQ=255)
                                                                                            ccTGCCAGCGATGGCAGGTTTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGCTTTGTc        <  2:1020088/100‑1 (MQ=255)
                                                                                            ccTGCCAGCGATGGCAGGTTTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGCTTTGTc        <  2:897351/100‑1 (MQ=255)
                                                                                             cTGCCAGCGATGGCAGGTTTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGCTTTGTca       <  2:74673/100‑1 (MQ=255)
                                                                                               gCCAGCGATGGCAGGTTTTTTTTTTTTTTTTTTTTGCGCACACCAATGCGAATATCTCCCTTATAAGTGTGTGGTTAGGTCAATAGAGAGTTTTGTca       >  1:1780892/1‑98 (MQ=255)
                                                                                                  aGCGATGGCAGGTTTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGCTTTGTCACAAtt  >  2:878557/1‑100 (MQ=255)
                                                                                                   |                                                                                                    
CGTATTCGTAAACGTCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGGTTTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGCTTTGTCACAATT  >  NC_000913/4060162‑4060359

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: