Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	4,168,372	G→C	100%	noncoding (1/76 nt)	gltT →	tRNA‑Glu

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,168,372	0	G	C	45.6%	‑7.0 / 2.4	6	noncoding (1/76 nt)	gltT	tRNA‑Glu
Reads supporting (aligned to +/- strand):  ref base G (0/2);  new base C (0/2);  total (1/5)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.35e-01

ATAAGGCCGTTCGCTTTCTATTAATGAAAGCTCACCCTACACGAAAATATCACGCAACGCGTGATAAGCAATTTTCGTGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACAGGGGTTCGAATCCCCTAGGGGACGCCACTTGCTGGTT  >  NC_000913/4168294‑4168457                                                                               |                                                                                      atacgcccGTTCGCTTTCTATTAATGAAAGCTCACCCTACACGAAAATATCACGCAACGCGTGATAAGCAATTTTCGTGTCCCCTTCGTCTAGAGGccc                                                                   <  1:674351/93‑1 (MQ=0)    agtccGTTCGCTTTCTATTAATGAAAGCTCACTCTACACGAAAATATCACGCAACGCGTGATAAGCAATTTTCGTGTCCCCTTCGTCTAGAGGCCCa                                                                  <  1:1788722/94‑1 (MQ=0)                         tgataactgaCCCTACGCGCAACTAGCTCTCCACGCGTAATAACCCATTCCCTTCTCCCCGTCGTCTCGATCCTGAGCACACCGCCCTTTCAcggcgg                                            <  1:663382/89‑1 (MQ=0)                                      tatacGAAAATATCACGCAACGCGTGATAAGCAATTTTCGGCTCCCCTTCGGCTAGAGGCCCAGGTCACCGCGCTTTCACGGCGGTAACAGGGGTTCGaa                             <  2:3567635/97‑1 (MQ=1)                                       acactagagtatCACGCAAGCCGTGATAAGAAATTTTCGTTGCCCCTTCGTCTAGGGGCCCAGGACACCGCCCTTTCACGGCGGTAACAGGGGTTCGaa                             <  1:3686023/90‑1 (MQ=1)                                                                 tacgCAATTTTCGTATCCCCTTCGTCTAGAGGCCCTGGACACCACCCTCTCACGGCTGTAACAGGGGTTCGAATCCCCGAGGGGACGCCACTTGCTGGtg  >  1:3603482/4‑99 (MQ=1)                                                                               |                                                                                      ATAAGGCCGTTCGCTTTCTATTAATGAAAGCTCACCCTACACGAAAATATCACGCAACGCGTGATAAGCAATTTTCGTGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACAGGGGTTCGAATCCCCTAGGGGACGCCACTTGCTGGTT  >  NC_000913/4168294‑4168457

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑